Abstract

A clinical sample testing system includes a portable apparatus for transport and incubation of a clinical sample in a culture vessel. The apparatus includes a sealable container having a thermally insulated compartment for receiving the vessel and a heater for heating the clinical sample to a temperature suitable for pre-culturing it. The system further includes a microorganism detection device which receives the vessel and cultures the clinical sample. The device includes a) a test aliquot extraction device for removing a test aliquot from the vessel and/or from a second culture vessel sampled therefrom, and a DNA testing device for separating DNA from the test aliquot and performing nucleic acid tests on the DNA to identify the microorganism and detecting genetic antimicrobial resistance markers therein and/or b) an antimicrobial susceptibility test device for performing an antimicrobial susceptibility test on the cultured clinical sample or portion thereof by monitoring microbial growth.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• A01N 1/02 - Preservation of living parts
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

An apparatus is for moving a sample holder on a platform from a loading position where the sample holder can be removed from the platform to a locked position where the sample holder is securely held. The sample holder is configured to rest on wheels within a recessed portion on the platform. The apparatus is configured such that: (i) movement of the platform from the loading position to the locked position causes a vertical clamp to lower down on top of the sample holder, and a horizontal clamp to be pressed to an outer periphery of the sample holder; and (ii) movement of the platform from the locked position to the loading position causes the vertical clamp to rise above the sample holder, and the horizontal clamp to be moved away from the outer periphery of the sample holder.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• G01N 21/07 - Centrifugal type cuvettes
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes other than for medical use; nucleic acid amplification reagents other than for medical use; nucleic acid sequencing programs other than for medical use; nucleic acid sequences, other than for medical or veterinary purposes; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; diagnostic preparations, other than for medical or veterinary purposes Diagnostic preparations for veterinary purposes; medical diagnostic reagents; clinical diagnostic reagents for medical or veterinary purposes; diagnostic preparations for medical purposes Scientific apparatus for detecting, identifying and analysing pathogens and infectious agents, and for microbial identification and antibiotic resistance marker mapping, all the aforesaid, for laboratory or medical research purposes; Scientific instruments for microbial identification and antibiotic resistance marker mapping; laboratory instruments for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes, for the detection and identification of pathogens for laboratory or research purposes; software for use in protein and nucleic acid analysis, micro-organism detection and identification, and in vitro diagnostics; downloadable computer operating system software; detection apparatus, namely, medical laboratory research apparatus for detecting pathogens or infectious agents; diagnostic apparatus, namely, fluorescence detectors; measuring instruments for measuring and quantifying DNA sequences; measuring apparatus for measuring and quantifying DNA sequences; testing apparatus for diagnostic purposes other than medical and veterinary use, namely, apparatus for testing antibiotic susceptibility of pathogens Medical diagnostic apparatus for the detection of abnormal prion protein; medical apparatus, namely, testing apparatus for analyzing bacteria in biological samples for medical use; medical diagnostic instruments for the detection and identification of pathogens or infectious agents; medical diagnostic apparatus for the detection and identification of pathogens or infectious agents; testing instruments for medical diagnostic purposes, for testing blood and other bodily fluids and biological samples; testing apparatus for testing blood and other bodily fluids and biological samples for medical and veterinary use Scientific analysis in the field of molecular biology; scientific research relating to molecular sciences; technological research relating to protein and nucleic acid analysis, microorganism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; technological scientific studies relating to protein and nucleic acid analysis, micro- organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; technological scientific consultancy relating to protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; scientific and scientific technological services, namely, protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, analysing and testing the antibiotic susceptibility of pathogens, microbial identification, and antibiotic resistance marker mapping; scientific consultancy and research services in the fields of science, engineering and information technology; provision of scientific information and data relating to scientific and technological research and development; research and development services in the field of bacteriology, cytology, antibody technology, chemistry, immunology and gene expression systems; scientific research; scientific research consultancy; scientific analysis in the field of molecular biology; scientific research services; scientific laboratory services; scientific genetic testing for scientific research purposes; scientific technological advisory services related to molecular biology and molecular sciences; compilation of scientific information; preparation of scientific reports for scientific analysis purposes; scientific research and development; conducting of scientific studies in the field of medicine; conducting of scientific experiments relating to pharmaceuticals; scientific research relating to bacteriology; scientific research relating to biology; scientific research in the field of genetics and genetic engineering; design and development of diagnostic apparatus; research and development in the field of diagnostic preparations; biotechnology testing relating to DNA for scientific research purposes; microbiological testing for scientific research purposes; bacteriological research and testing for scientific research purposes; analytical services relating to the testing of the bacterial content of liquids for scientific research purposes; medical testing for research purposes Rental of medical equipment; consultancy services in the fields of medical and orthopaedic surgery; consultancy services in the fields of medical diagnostic testing, monitoring and reporting services, namely, protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, detection, identification and analysis of pathogens and infectious agents, antibiotic susceptibility of pathogens, microbial identification, and antibiotic resistance marker mapping, all for the diagnostic treatment of patients; Medical diagnostic testing, monitoring and reporting services; surgical diagnostic services being medical diagnostic testing, monitoring and reporting services; medical testing for diagnostic and treatment purposes, medical services for the testing of blood; medical services for the testing of sera; medical and healthcare services in the fields of DNA, genetics and genetic testing; medical testing services relating to the diagnosis and treatment of disease

Abstract

A method for performing microscopy-based imaging of samples includes: loading a sample holder onto a support configured to receive the sample holder; moving the sample holder in a first direction, from a starting position on a first strip of the sample holder, to move the sample holder relative to an imaging line of a line camera, to capture an image of the first strip of the sample holder; monitoring a focal plane using an autofocus system as the sample holder is moved in the first direction; in response to a determination by the autofocus system, moving an objective lens along an optical axis to adjust the focal plane; and moving the sample holder in a second direction, to align the imaging line of the line camera with a position on a second strip of the sample holder.

IPC Classes  ?

• G02B 21/00 - Microscopes
• G02B 21/24 - Base structure
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G02B 21/26 - StagesAdjusting means therefor

Abstract

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.

IPC Classes  ?

• C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12M 1/26 - Inoculator or sampler
• G01N 1/40 - Concentrating samples

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus, not for medical purposes; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment for medical purposes; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes.

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids for laboratory use; nucleic acid for laboratory use, namely, for use in nucleic acid amplification processes not for medical use; nucleic acid amplification reagents for scientific and research use; nucleic acid sequencing programs being nucleic acid sequences other than for medical and veterinary purposes; nucleic acid sequences, other than for medical or veterinary purposes; chemical substances for analyses in laboratories, other than for medical or veterinary purposes, namely, chemicals for use in multiplying nucleic acid amounts; diagnostic kits consisting primarily of diagnostic reagents for scientific use being reagents for testing for selected bacteria, and also comprising specimen receptors being scientific research specimen collection devices in the nature of tubes and flasks for the collection of blood, sterile body fluids, and urine; diagnostic preparations for scientific purposes; chemical products for diagnostic use, namely, chemical preparations for analyses in laboratories, other than medical or veterinary purposes; chemical diagnostic reagents for scientific use; diagnostic preparations, other than for medical or veterinary purposes Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations for medical purposes; medical diagnostic reagents; clinical diagnostic reagents for medical purposes; diagnostic preparations for medical purposes Scientific apparatus, namely, incubators being laboratory equipment, spectrophotometers for measuring relative DNA, RNA and protein, stirring devices for scientific and laboratory use; magnetic agitators for laboratory use, and light detectors for detecting and identifying chemical and biological substances not for medical purposes; probes for scientific purposes; scientific apparatus and instruments, namely, incubators being laboratory equipment, spectrophotometers for measuring relative DNA, RNA and protein, magnetic agitators for laboratory use; scientific, optical, measuring, signaling and checking apparatus and instruments, namely, incubators being laboratory equipment, spectrophotometers for measuring DNA, RNA and protein, magnetic agitators for laboratory use; scientific research and laboratory apparatus, namely, incubators being laboratory equipment, spectrophotometers for measuring DNA, RNA and protein, magnetic agitators for laboratory use, testing apparatus for diagnostic purposes other than medical, namely, apparatus for detection and identification of pathogens; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments, namely, incubators being laboratory equipment, magnetic agitators for laboratory use, laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes, namely, diagnostic apparatus for testing food, diagnostic apparatus for the detection of pathogens for laboratory or research use; downloadable and recorded software for use in connection with the aforementioned scientific, research, laboratory apparatus and instruments, namely, incubators, spectrophotometers, stirring devices, agitators and light detectors for analyzing and interpreting signals from said apparatus; computer systems comprised of computers, computer peripherals, and recorded computer operating programs; infrared detection apparatus, not for medical purposes Medical diagnostic apparatus for testing being the detection of abnormal prion protein; medical diagnostic apparatus for testing being analyzing of bacteria in biological samples; medical diagnostic instruments for analyzing of bacteria in biological samples; tools for medical diagnostics, namely, diagnostic apparatus for analyzing of bacteria in biological samples; diagnostic apparatus for medical purposes for testing being analyzing of bacteria in biological samples; diagnostic, examination, and monitoring equipment for medical purposes, namely, for testing being the detection of abnormal prion protein and analyzing of bacteria in biological samples; testing instruments for medical diagnostic purposes, namely, for the detection of abnormal prion protein and analyzing of bacteria in biological samples; testing probes for medical diagnostic purposes; testing apparatus for medical purposes, namely, for analyzing of bacteria in biological samples

Abstract

A portable apparatus and method for transport and incubation of a medical sample in a blood culture flask includes a sealable container having a thermally insulated compartment for receiving the blood culture flask and a heater for heating the medical sample to a temperature suitable for pre-culturing of the sample. An agitator is provided for agitating the sample in the blood culture flask.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• A01N 1/02 - Preservation of living parts
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes.

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes.

Abstract

A method of image-based analysis of multiple samples includes using a sample holder having multiple locations of interest and multiple focal structures that are each associated, one or more, with the multiple locations of interest, wherein the multiple samples are dispersed across the multiple locations of interest and obtaining image areas of the multiple locations of interest. Multiple digital image areas are thus obtained for use in an analysis of the multiple samples with each of the image areas including at least one of the locations of interest and at least one of the focal structures. An image processing algorithm is used to analyse each of the digital image areas and check if the focal structure indicates that the image area is in clear focus. An indication is provided and/or remedial action is taken if the image processing algorithm indicates that any digital image areas are out of focus.

IPC Classes  ?

• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G06T 7/00 - Image analysis
• G06T 7/70 - Determining position or orientation of objects or cameras
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G06T 5/50 - Image enhancement or restoration using two or more images, e.g. averaging or subtraction
• H04N 5/232 - Devices for controlling television cameras, e.g. remote control

Abstract

An apparatus is for moving a sample holder on a platform from a loading position where the sample holder can be removed from the platform to a locked position where the sample holder is securely held. The sample holder is configured to rest on wheels within a recessed portion on the platform. The apparatus is configured such that: (i) movement of the platform from the loading position to the locked position causes a vertical clamp to lower down on top of the sample holder, and a horizontal clamp to be pressed to an outer periphery of the sample holder; and (ii) movement of the platform from the locked position to the loading position causes the vertical clamp to rise above the sample holder, and the horizontal clamp to be moved away from the outer periphery of the sample holder.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• G01N 21/07 - Centrifugal type cuvettes
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Abstract

An analysis instrument comprising a cartridge bay 20 for receiving a sample preparation cartridge 1, wherein the cartridge bay comprises: a first controllable heater 26 for heating a sample; a first temperature sensor for monitoring the temperature of the first controllable heater; and a second temperature sensor 28 for monitoring the temperature of the sample.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

A sample holder (10) comprises a sample chamber (33), a gas reservoir (32) and an upper layer (20) covering over the sample chamber (33) and gas reservoir (32), wherein a bottom surface of the upper layer (20) comprises a microstructure array (23) which overlies at least a portion of a top periphery of the sample chamber (33), and wherein the microstructure array (23) is in communication with a gas path which extends to the gas reservoir (32), to allow gas exchange between the sample chamber (33) and the gas reservoir (32).

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• F28F 3/12 - Elements constructed in the shape of a hollow panel, e.g. with channels
• B01L 9/00 - Supporting devicesHolding devices
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12Q 1/18 - Testing for antimicrobial activity of a material
• G01N 21/03 - Cuvette constructions

Abstract

The present invention provides a method of preparing a suspension of intact microorganisms from a sample containing microorganisms and mammalian cells, comprising contacting the sample with a buffer solution with a pH of at least pH 6 and less than pH 9, a detergent and one or more proteases to allow lysis of the mammalian cells; filtering the mixture through a filter suitable for retaining microorganisms to remove the lysed mammalian cells; resuspending the microorganisms retained by the filter in a liquid to provide a suspension comprising the recovered intact microorganisms; and determining the concentration of microorganisms in the suspension by: i. heating and/or contacting an aliquot of the suspension with an alcohol; ii. optionally diluting one or more aliquots of the suspension to provide one or more diluted aliquots before, during and/or after step (i); iii. contacting at least a portion of an aliquot of step (i) or (ii) with a single fluorescent stain capable of binding to DNA; iv. imaging the mixture of step (iii) at the emission wavelength of the fluorescent stain and determining an image analysis value for the number of objects corresponding to microorganisms in the imaged mixture; and v. comparing the image analysis value to a pre-determined calibration curve, thereby to determine the concentration of microorganisms in the suspension.

IPC Classes  ?

• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.

IPC Classes  ?

• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12M 1/26 - Inoculator or sampler
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).

IPC Classes  ?

• G02B 21/00 - Microscopes
• G02B 21/24 - Base structure
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G02B 21/26 - StagesAdjusting means therefor

Abstract

A sample holder (10) comprises: an upper layer (20); a lower layer (40); a middle layer (30) between the upper and lower layers; and a sample chamber (33) formed by a through-hole in the middle layer (30), covered at its upper extent by a portion of the bottom surface of the upper layer (20), and at its lower extent by a portion of the top surface of the lower layer (40), wherein at least part of the bottom surface of the upper layer (20) overlapping a portion of a top periphery of the sample chamber (33) comprises a hydrophobic surface, wherein the hydrophobic surface is sufficiently hydrophobic that a contact angle of a water droplet on the hydrophobic surface would exceed 110°.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12Q 1/18 - Testing for antimicrobial activity of a material
• G01N 21/03 - Cuvette constructions

Abstract

A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor

Abstract

The present invention relates to a method of determining the concentration of intact microorganisms in a sample comprising optionally diluting an aliquot of the sample to provide a diluted aliquot at a dilution value; contacting at least a portion of an aliquot or of a diluted aliquot of the sample with first and second stains capable of binding to DNA, wherein the first stain is a fluorescent stain, is cell-permeable, and has a first emission wavelength, and the second stain is cell-impermeable, and is capable of acting as an acceptor molecule in a FRET pair with the first stain acting as a donor molecule; imaging the aliquot-stain mixture at the first emission wavelength and determining an image analysis value for the number of objects corresponding to intact microorganisms in the imaged mixture; and comparing the image analysis value for said aliquot to a pre-determined calibration curve, as well as an apparatus, a consumable and a kit therefor.

IPC Classes  ?

• C12Q 1/06 - Quantitative determination
• G01N 1/38 - Diluting, dispersing or mixing samples
• G01N 1/31 - Apparatus therefor
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/26 - Inoculator or sampler
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• C12N 1/06 - Lysis of microorganisms
• G01N 21/64 - FluorescencePhosphorescence

Abstract

Multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence are described. The target sequence is either part of an analyte or indicative of the presence of an analyte in the sample. Each padlock probe includes an analyte-specific reporter sequence, and either a restriction cleavage site located 3′ of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction. Where the padlock probe includes a restriction cleavage site, cleavage at the restriction cleavage site occurs 3′ of the analyte-specific reporter sequence. Where the padlock probe includes a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5′ of the analyte-specific reporter sequence. Panels of probes and kits for the same are also described.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

A method for analysis of a digital image in order to determine a value relating to an amount of a substance shown in the image includes using a threshold to identify all pixels of the digital image above or below a certain intensity value in order to produce a first binary image. Texture filtering is used to identify regions of high texture in the digital image to produce a second binary image in which all regions of high texture are pixels with a first state. The first and second binary images are overlayed to produce a combined binary image. A value relating to the amount of the substance is determined based on the area of the combined binary image with pixels having the first state.

IPC Classes  ?

• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G06T 7/00 - Image analysis
• G02B 21/24 - Base structure

Abstract

A sample holder 100 for use in a device for image-based analysis of multiple samples, the sample holder comprising: an optically flat surface 10; multiple locations of interest 16, 18 dispersed across the optically flat surface 10 that each, in use, correspond to locations of a sample of the multiple samples; and multiple focal structures 12 associated with the multiple locations of interest 16, 18; wherein the focal structures 12 each comprise at least one pyramid shaped indentation 30 in the optically flat surface 10.

IPC Classes  ?

• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G02B 27/40 - Optical focusing aids
• G01N 21/64 - FluorescencePhosphorescence
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

An apparatus for moving a sample holder (110) on a platform (652) from a loading position, where the sample holder (110) can be removed from the platform (652), to a locked position, where the sample holder (110) is securely held, wherein the sample holder (110) rests on wheels (690a) within a recessed portion (651) on the platform (652), wherein the apparatus is configured such that movement from the loading position to the locked position causes a vertical clamping means (653) to lower down on top of the sample holder (110), and a horizontal clamping (657) means to be pressed to the outer periphery of the sample holder (110), and such that that movement from the locked position to the loading position causes the vertical clamping means (653) to raise above the sample holder (110), and the horizontal clamping means (657) to be moved away from the outer periphery of the sample holder (110).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• B01L 9/00 - Supporting devicesHolding devices
• G01N 21/07 - Centrifugal type cuvettes
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/18 - Testing for antimicrobial activity of a material
• G01N 1/30 - StainingImpregnating
• G01N 1/40 - Concentrating samples
• G01N 21/64 - FluorescencePhosphorescence

Abstract

An analysis instrument comprising a cartridge bay (20) for receiving a sample preparation cartridge, wherein the cartridge bay comprises: a first controllable heater (26) for heating a sample; a first temperature sensor for monitoring the temperature of the first controllable heater; and a second temperature sensor for monitoring the temperature of the sample.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

An apparatus for moving a sample holder (110) on a platform (652) from a loading position, where the sample holder (110) can be removed from the platform (652), to a locked position, where the sample holder (110) is securely held, wherein the sample holder (110) rests on wheels (690a) within a recessed portion (651) on the platform (652), wherein the apparatus is configured such that movement from the loading position to the locked position causes a vertical clamping means (653) to lower down on top of the sample holder (110), and a horizontal clamping (657) means to be pressed to the outer periphery of the sample holder (110), and such that that movement from the locked position to the loading position causes the vertical clamping means (653) to raise above the sample holder (110), and the horizontal clamping means (657) to be moved away from the outer periphery of the sample holder (110).

IPC Classes  ?

• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• B01L 9/00 - Supporting devicesHolding devices
• G01N 21/07 - Centrifugal type cuvettes
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/18 - Testing for antimicrobial activity of a material
• G01N 1/30 - StainingImpregnating
• G01N 1/40 - Concentrating samples
• G01N 15/06 - Investigating concentration of particle suspensions
• G01N 21/64 - FluorescencePhosphorescence

Abstract

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.

IPC Classes  ?

• C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12M 1/26 - Inoculator or sampler
• G01N 1/40 - Concentrating samples

Abstract

The present invention provides a method of preparing a suspension of intact microorganisms from a sample containing microorganisms and mammalian cells,comprising contacting the sample with a buffer solution with a pH of at least pH 6 and less than pH 9, a detergent and one or more proteases to allow lysis of the mammalian cells; filtering the mixture through a filter suitable for retaining microorganisms to remove the lysed mammalian cells; resuspending the microorganisms retained by the filter in a liquid to provide a suspension comprising the recovered intact microorganisms; and determining the concentration of microorganisms in the suspension by:i.heating and/or contacting an aliquot of the suspension with an alcohol;ii.optionally diluting one or more aliquots of the suspension to provide one or more diluted aliquots before, during and/or after step (i);iii.contacting at least a portion of an aliquot of step (i) or (ii) with a single fluorescent stain capable of binding to DNA; iv.imaging the mixture of step (iii) at the emission wavelength of the fluorescent stain and determining an image analysis value for the number of objects corresponding to microorganisms in the imaged mixture; and v.comparing the image analysis value to a pre- determined calibration curve, thereby to determine the concentration of microorganisms in the suspension.

IPC Classes  ?

• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• G16H 10/00 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data

Abstract

A sample holder (10) comprises a sample chamber (33), a gas reservoir (32) and an upper layer (20) covering over the sample chamber (33) and gas reservoir (32), wherein a bottom surface of the upper layer (20) comprises a microstructure array (23) which overlies at least a portion of a top periphery of the sample chamber (33), and wherein the microstructure array (23) is in communication with a gas path which extends to the gas reservoir (32), to allow gas exchange between the sample chamber (33) and the gas reservoir (32).

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

A sample holder (10) comprises: an upper layer (20); a lower layer (40); a middle layer (30) between the upper and lower layers; and a sample chamber (33) formed by a through-hole in the middle layer (30), covered at its upper extent by a portion of the bottom surface of the upper layer (20), and at its lower extent by a portion of the top surface of the lower layer (40), wherein at least part of the bottom surface of the upper layer (20) overlapping a portion of a top periphery of the sample chamber (33) comprises a hydrophobic surface, wherein the hydrophobic surface is sufficiently hydrophobic that a contact angle of a water droplet on the hydrophobic surface would exceed 110°.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

A method for performing microscopy-based imaging of samples comprises: loading a sample holder (100) onto a support (50) configured to receive the sample holder (100); moving the sample holder (100) in a first direction, from a starting position on a first strip of the sample holder (100), to move the sample holder (100) relative to an imaging line of a line camera (10), to capture an image of the first strip of the sample holder (100); monitoring a focal plane using an autofocus system (15) as the sample holder (100) is moved in the first direction; in response to a signal from the autofocus system (15), moving an objective lens (25) along the optical axis to adjust the focal plane; and moving the sample holder (100) in a second direction, to align the imaging line of the line camera (10) with a position on a second strip of the sample holder (100).

IPC Classes  ?

• G02B 21/00 - Microscopes
• G02B 21/24 - Base structure
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• H04N 1/053 - Detection, control or error compensation of scanning velocity or position in main scanning direction, e.g. synchronisation of line start or picture elements in a line
• H04N 1/19 - Scanning arrangements using multi-element arrays
• H04N 1/193 - Simultaneously scanning picture elements on one main scanning line using electrically scanned linear arrays
• H04N 1/195 - Scanning arrangements using multi-element arrays the array comprising a two-dimensional array
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B29C 45/26 - Moulds

Abstract

A method for detecting and characterising a microorganism in a clinical sample includes introducing the clinical sample to a first culture vessel and simultaneously transporting and incubating the clinical sample in a thermally insulated compartment of a sealable container. A test aliquot is removed from the sample and the remainder of the sample is further cultured. DNA is removed from the test aliquot and nucleic acid tests are performed on it to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. One or more nucleic acid probes or primers are used to perform the nucleic acid tests. It is detected whether or not the probes or primers have hybridised to DNA or the primers have been extended. If a microorganism is identified, then an antimicrobial susceptibility test is performed on the cultured clinical sample.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• A01N 1/02 - Preservation of living parts
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

The present invention relates to a method of determining the concentration of intact microorganisms in a sample comprising optionally diluting an aliquot of the sample to provide a diluted aliquot at a dilution value; contacting at least a portion of an aliquot or of a diluted aliquot of the sample with first and second stains capable of binding to DNA, wherein the first stain is a fluorescent stain, is cell- permeable, and has a first emission wavelength, and the second stain is cell- impermeable, and is capable of acting as an acceptor molecule in a FRET pair with the first stain acting as a donor molecule; imaging the aliquot-stain mixture at the first emission wavelength and determining an image analysis value for the number of objects corresponding to intact microorganisms in the imaged mixture; and comparing the image analysis value for said aliquot to a pre-determined calibration curve, as well as an apparatus, a consumable and a kit therefor.

IPC Classes  ?

• C12Q 1/06 - Quantitative determination
• G01N 33/542 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
• G01N 21/64 - FluorescencePhosphorescence

Abstract

A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.

IPC Classes  ?

• G01N 1/00 - SamplingPreparing specimens for investigation
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G02B 27/40 - Optical focusing aids
• G01N 21/64 - FluorescencePhosphorescence
• B29L 31/00 - Other particular articles
• B29C 45/26 - Moulds

Abstract

A method for determining the antimicrobial susceptibility of a microorganism in a clinical sample includes removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units and isolating the microbial cells. The cells are transferred into a suitable culture medium for microbial growth and an AST assay is performed using the isolated microbes. The concentration of microbial cells in the microbial cells used to set up the AST assay is measured before measuring the degree of microbial growth in different conditions of the AST assay. Devices for determining the anti-microbial susceptibility of a microorganism in a clinical sample are also disclosed.

IPC Classes  ?

• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12M 1/26 - Inoculator or sampler
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

The present invention relates to multiplexed methods of detecting an analyte in a sample using two or more padlock probes each specific to a different target sequence, the target sequence being either part of an analyte or indicative of the presence of an analyte in the sample, wherein each padlock probe comprises an analyte-specific reporter sequence, and either a restriction cleavage site located 3' of the analyte-specific reporter sequence, and/or a first amplification primer binding site for an amplification reaction, wherein where the padlock probe comprises a restriction cleavage site, cleavage at said restriction cleavage site occurs 3' of the analyte-specific reporter sequence, and where the padlock probe comprises a first amplification primer binding site for the further amplification reaction, it does not contain a second amplification primer binding site 5' of the analyte-specific reporter sequence, and panels of probes and kits for the same.

IPC Classes  ?

• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates
• C12Q 1/6823 - Release of bound markers

Abstract

A portable apparatus for transport and incubation of a medical sample in a blood culture flask includes a sealable container having a thermally insulated compartment for receiving the blood culture flask and a heater for heating the medical sample to a temperature suitable for pre-culturing of the sample. An agitator is provided for agitating the sample in the blood culture flask.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/00 - Apparatus for enzymology or microbiology
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• A01N 1/02 - Preservation of living parts
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor

Abstract

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d)recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.

IPC Classes  ?

• C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism
• C12N 1/20 - BacteriaCulture media therefor

Abstract

A sample holder 100 for use in a device for image-based analysis of multiple samples, the sample holder comprising: an optically flat surface 10; multiple locations of interest 16, 18 dispersed across the optically flat surface 10 that each, in use, correspond to locations of a sample of the multiple samples; and multiple focal structures 12 associated with the multiple locations of interest 16, 18; wherein the focal structures 12 each comprise at least one pyramid shaped indentation 30 in the optically flat surface 10.

IPC Classes  ?

• G02B 21/24 - Base structure
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G02B 27/40 - Optical focusing aids

Abstract

A method of image-based analysis of multiple samples comprises: using a sample holder 100 having multiple locations of interest 16, 18 and multiple focal structures 12 that are each associated one or more with the multiple locations of interest 16, 18, wherein the multiple samples are dispersed across the multiple locations of interest 16, 18; obtaining image areas of the multiple locations of interest 16, 18, thereby obtaining multiple digital image areas for use in analysis of the multiple samples with each of the image areas including at least one of the locations of interest 16, 18 and at least one of the focal structures 12; using an image processing algorithm to analyse each of the digital image areas and check if the focal structure 12 indicates that the image area is in clear focus; and providing an indication and/or taking remedial action if the image processing algorithm indicates that any digital image areas are out of focus.

IPC Classes  ?

• G02B 27/32 - Fiducial marks or measuring scales within the optical system
• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G02B 7/36 - Systems for automatic generation of focusing signals using image sharpness techniques

Abstract

A method for analysis of a digital image 12 in order to determine a value relating to the amount of a substance shown in the image, wherein in the digital image the presence of the substance can be identified based on the intensity of pixels of the digital image, the method comprising: using a threshold 14 to identify all pixels of the digital image 12 above or below a certain intensity value in order to produce a first binary image in which regions of high concentration of the substance are pixels with a first state of first and second states of the pixels of the first binary image, for example pixels representable as black pixels; using texture filtering 16 to identify regions of high texture in the digital image 12 and produce a second binary image in which all regions of high texture are pixels with a first state of first and second states of the pixels of the second binary image, for example pixels representable as black pixels; overlaying the first and second binary images to produce a combined binary image 18, wherein the pixels with the first state in one or both of the first and second binary images are pixels with a first state of first and second states of the pixels of the combined binary image, for example pixels representable as black in the combined binary image 18, and the pixels with the second state in both of the first and second binary images are pixels with a second state of first and second states of the pixels of the combined binary image 18, for example pixels representable as white pixels in the combined binary image; and determining the value relating to the amount of the substance based on the area of the combined binary image with pixels having the first state.

IPC Classes  ?

• G06T 7/00 - Image analysis

Abstract

A method for detecting and characterising a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel and then simultaneously transporting and incubating the clinical sample in a thermally insulated compartment of a sealable container. A test aliquot is removed from the sample and the remainder of the sample is cultured further. The method further includes removing DNA from said test aliquot and performing nucleic acid tests on said DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in said microorganism. The nucleic acid tests are performed using: i) one or more nucleic acid probes and/or primers for microbial identification, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence which is identificatory of a given microorganism; and ii) one or more nucleic acid probes and/or primers for antimicrobial resistance marker detection, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence representing a genetic antimicrobial resistance marker. It is detected whether or not said probes and/or primers have hybridised to said DNA and/or said primers have been extended (e.g. an amplification reaction has taken place) and if a microorganism is identified in step then an antimicrobial susceptibility test is performed on said cultured clinical sample.

IPC Classes  ?

• A01N 1/02 - Preservation of living parts
• B65D 81/38 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for scientific diagnostic use; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes [ Diagnostic preparations for medical purposes; medical diagnostic reagents; clinical diagnostic reagents for medical use; diagnostic preparations for medical purposes ] Scientific, optical, measuring, signaling and checking apparatus and instruments, namely, microscopes for microbe detection and susceptibility determination, identification, analyzing and testing; scientific research and laboratory apparatus, namely, microscopes for microbe detection and susceptibility determination, identification, analyzing and testing; laboratory instruments, namely, microscopes for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes, namely, microscopes for microbe detection and susceptibility determination, identification, analyzing and testing; software for analyzing pathogen and susceptibility detection, identification, analyzing and testing; computer systems primarily comprised of computers and computer hardware and also including computer software for analyzing laboratory experiment data; detection apparatus, namely medical laboratory research instruments, namely, microscopes used to test for microbe detection and susceptibility determination; measuring apparatus and instruments, namely pathogen growth measuring instruments; testing apparatus for diagnostic purposes for testing food, other than medical Apparatus for detection and analysis of microbes in biological samples for medical use; medical diagnostic instruments for testing for microbes in human and veterinary body fluids; tools for medical diagnostics for testing for infectious diseases and antimicrobial susceptibility; diagnostic apparatus for medical purposes for use in diagnosing infectious diseases; diagnostic, examination, and monitoring equipment, all being for medical use, for antimicrobial susceptibility testing; testing instruments for detecting, identification and analysis of microbes for medical purposes; none of the aforesaid being for use in the design or creation of personalized joint replacement technologies

Goods & Services

(Based on 44(e)) Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids for laboratory, scientific or research use; nucleic acid for use in nucleic acid amplification processes other than for medical and veterinary use; nucleic acid amplification reagents other than for medical and veterinary use; nucleic acid sequencing programs other than for medical and veterinary use; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts other than medical and veterinary use; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for scientific diagnostic use; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes (Based on 44(e)) diagnostic preparations for medical and veterinary purposes; medical diagnostic reagents; clinical diagnostic reagents for medical and veterinary purposes; diagnostic preparations for medical purposes (Based on 44(e)) Scientific, optical, measuring, and checking apparatus and instruments for microbe detection, identification and susceptibility determination, identification, analyzing and testing, namely, fluidics and time-lapse imaging apparatus not for medical purposes; scientific research and laboratory apparatus for microbe detection and susceptibility determination, identification, analyzing and testing, namely, for fluidics and time-lapse imaging; laboratory instruments, namely, fluidics and time-lapse imaging devices for the detection of pathogens in a biological sample for research use; diagnostic apparatus, not for medical purposes, for microbe detection and susceptibility determination, identification, analyzing and testing for laboratory or research use; software for analyzing pathogen and susceptibility detection, identification, analyzing and testing for microbe detection and susceptibility determination; measuring apparatus and instruments, namely, pathogen growth measuring instruments not for medical purposes (Based on 44(e)) Apparatus for detection and analysis of microbes in biological samples for medical use; medical diagnostic instruments for testing for microbes in human and veterinary body fluids; diagnostic apparatus, for medical diagnostics for testing for infectious diseases and antimicrobial susceptibility; diagnostic apparatus for medical purposes for use in diagnosing infectious diseases; diagnostic, examination, and monitoring equipment which operates via time-lapse imaging for antimicrobial susceptibility testing all being for medical purposes; diagnostic testing apparatus for detecting, identification and analysis of microbes for medical purposes; none of the aforesaid being for use in the design or creation of personalized joint replacement technologies or for interfacing with living tissue, namely, stimulating, acquiring, recording, analyzing and using signals from the brain, nervous system and related structures

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes; none of the aforesaid being for use in the design or creation of personalised joint replacement technologies.

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes;  All the aforementioned for use in connection with an antibiotic susceptibility testing product. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes; none of the aforesaid being for use in the design or creation of personalised joint replacement technologies.

Abstract

A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids for laboratory use; nucleic acid for use in nucleic acid amplification processes other than for medical and veterinary use; nucleic acid amplification reagents other than for medical and veterinary use; nucleic acid sequencing programs other than for medical and veterinary use; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts other than medical and veterinary use; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for scientific diagnostic use; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations for veterinary purposes; medical diagnostic reagents; clinical diagnostic reagents for medical or veterinary purposes; diagnostic preparations for medical purposes Scientific apparatus for detecting, identifying and analyzing pathogens and infectious agents, and for microbial identification and antibiotic resistance marker mapping, all the aforesaid, for laboratory or medical research purposes; Scientific instruments for microbial identification and antibiotic resistance marker mapping; laboratory instruments for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes, for the detection and identification of pathogens for laboratory or research purposes; software for use in protein and nucleic acid analysis, micro-organism detection and identification, and in vitro diagnostics; computer operating systems; detection apparatus, namely, medical laboratory research apparatus for detecting pathogens or infectious agents; diagnostic apparatus, namely, fluorescence detectors; measuring instruments for measuring and quantifying DNA sequences; measuring apparatus for measuring and quantifying DNA sequences; testing apparatus for diagnostic purposes other than medical and veterinary use, namely, apparatus for testing antibiotic susceptibility of pathogens Diagnostic apparatus for the detection of abnormal prion protein for medical use for the detection of cancer; medical apparatus, namely, testing apparatus for analyzing bacteria in biological samples for medical use; medical diagnostic instruments for the detection and identification of pathogens or infectious agents; diagnostic apparatus for the detection and identification of pathogens or infectious agents for medical use; testing instruments for medical diagnostic purposes, for testing blood and other bodily fluids and biological samples; testing apparatus for testing blood and other bodily fluids and biological samples for medical and veterinary use Scientific analysis in the field of molecular biology; scientific research relating to molecular sciences; technological scientific research relating to protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; technological scientific studies relating to protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; technological scientific consultancy relating to protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, the antibiotic susceptibility of pathogens, microbial identification and antibiotic resistance marker mapping; scientific and scientific technological services, namely, protein and nucleic acid analysis, micro-organism detection and identification, in vitro diagnostics, the detection, identification and analysis of pathogens and infectious agents, analyzing and testing the antibiotic susceptibility of pathogens, microbial identification, and antibiotic resistance marker mapping; scientific consultancy and research services in the fields of science, engineering and information technology; provision of scientific information and data relating to scientific and technological research and development; research and development services in the field of bacteriology, cytology, antibody technology, chemistry, immunology and gene expression systems; scientific research; scientific consultancy relating to dietary supplements; scientific analysis in the field of molecular biology; scientific research services; scientific laboratory services; scientific genetic testing for scientific research purposes; scientific advisory services relating to carbon offsetting; compilation of scientific information; preparation of scientific reports for scientific analysis purposes; scientific research and development; conducting of scientific studies in the field of medicine; providing laboratory research services featuring the conducting of scientific experiments relating to pharmaceuticals; scientific research relating to bacteriology; scientific research relating to biology; scientific research in the field of genetics and genetic engineering; design and development of diagnostic apparatus; research and development in the field of diagnostic preparations; biotechnology testing relating to DNA, for scientific research purposes; microbiological testing for scientific research purposes; bacteriological research and testing for scientific research purposes; analytical services relating to the testing of the bacterial content of liquids for scientific research purposes; medical testing for research purposes Rental of apparatus and installations in the field of medical technology, namely, rental of medical equipment; consultancy services in the fields of medical and orthopedic surgery; medical diagnostic testing, monitoring and reporting services; surgical diagnostic services in the nature of exploratory surgery services for use in diagnosing medical illness and conditions; medical testing for diagnostic and treatment purposes, medical services for the testing of blood; medical services for the testing of sera; medical and healthcare services in the fields of DNA, genetics and genetic testing; medical testing services relating to the diagnosis and treatment of disease

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes. Analysis in the field of molecular biology; research relating to molecular sciences; technological research; technological studies; technological consultancy; technological services; scientific and technological services; consultancy services relating to technological research; scientific and technological services and research; scientific and technological services and research and development relating thereto; scientific and technological services and research and design services related thereto; consultancy and research services in the fields of science, engineering and information technology; provision of information and data relating to scientific and technological research and development; research and development services in the field of bacteriology, cytology, antibody technology, chemistry, immunology and gene expression systems; scientific research; scientific consultancy; scientific analysis; research (scientific -); scientific research services; laboratory (scientific -) services; scientific testing services; scientific advisory services; scientific laboratory services; compilation of scientific information; preparation of scientific reports; scientific research and development; conducting of scientific studies; conducting of scientific experiments; scientific research relating to bacteriology; scientific research relating to biology; scientific research in the field of genetics and genetic engineering; design and development of diagnostic apparatus; research and development in the field of diagnostic preparations; biotechnology testing; microbiological testing; bacteriological research and testing; analytical services relating to the testing of the bacterial content of liquids; professional consultancy in the field of medical technology. Rental of apparatus and installations in the field of medical technology; professional consultancy in the field of medical surgery and orthopaedics; medical diagnostic services; medical and surgical diagnostic services; medical diagnostic services [testing and analysis]; medical testing; services for the testing of blood; services for the testing of sera; medical and health services relating to dna, genetics and genetic testing; medical testing services relating to the diagnosis and treatment of disease.

Abstract

The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.

IPC Classes  ?

• C12Q 1/18 - Testing for antimicrobial activity of a material

Abstract

A portable apparatus for transport and incubation of a medical sample in a blood culture flask (2), comprises: a sealable container (1) having a thermally insulated compartment (3) for receiving the blood culture flask (2); a heater (11) for heating the medical sample to a temperature suitable for pre-culturing of the sample; and an agitator (16) for mechanically agitating the blood culture flask (2).

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation
• A01N 1/02 - Preservation of living parts
• B65D 81/38 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
• G01N 1/38 - Diluting, dispersing or mixing samples
• G01N 1/42 - Low-temperature sample treatment, e.g. cryofixation

Abstract

A portable apparatus for transport and incubation of a medical sample in a blood culture flask (2), comprises: a sealable container (1) having a thermally insulated compartment (3) for receiving the blood culture flask (2); a heater (11) for heating the medical sample to a temperature suitable for pre-culturing of the sample; and an agitator (16) for mechanically agitating the blood culture flask (2).

IPC Classes  ?

• A01N 1/02 - Preservation of living parts
• B65D 81/38 - Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation

Abstract

A method for performing a rolling circle amplification (RCA) reaction includes at least two rounds of RCA. The method includes providing a concatemeric first RCA product having a multiplicity of monomer repeats, each repeat representing a complementary copy of a first RCA template, wherein the nucleic acid molecule to be detected and/or analyzed, or its compliment, is contained in the first RCA template. The first RCA product is cleaved into monomer units which are reduced in size compared to the monomer repeat of the first RCA template. Monomer units resulting from the cleavage are circularized to form second RCA templates, which are smaller than the first RCA template. A second RCA reaction is performed on the second RCA template and a primer for the second RCA to form a second RCA product. The second RCA product, or a monomer unit derived from it, is detected or analyzed.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/682 - Signal amplification
• C12Q 1/6853 - Nucleic acid amplification reactions using modified primers or templates

Abstract

The present invention provides a method for detecting and characterising a microorganism in aclinical sample, said method comprising: a) introducing a clinical sample to a first culture vessel containing culture medium; b (i) optionally preculturing said clinical sample in said first culture vessel; (ii) optionally removing a portion of the clinical sample/medium mixture or, if precultured, the clinical sample culture from said first culture vessel, and introducing said portion to a second culture vessel containing culture medium, and optionally preculturing said portion in said second culture vessel; c) removing a test aliquot from said first and/or second culture vessel, and culturing or continuing to culture said clinical sample and/or portion in said first and/or second culture vessel; d) separating DNA from said test aliquot; e) performing nucleic acid tests on said DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in said microorganism, wherein said nucleic acid tests are performed using: i) one or more nucleic acid probes and/or primers for microbial identification, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence which is identificatory of a given microorganism; and ii) one or more nucleic acid probes and/or primers for antimicrobial resistance marker detection, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence representing a genetic antimicrobial resistance marker; and it is detected whether or not said probes and/or primers have hybridised to said DNA and/or said primers have been extended (e.g. an amplification reaction has taken place); and f) if a microorganism is identified in step (e), performing an antimicrobial susceptibility test on said cultured clinical sample and/or portion from step (c), wherein microbial growth in said antimicrobial susceptibility test is monitored by assessing growth or markers for growth, and wherein the type and concentration of antimicrobial agents used in said antimicrobial susceptibility test is determined by the identity of the microorganism and antimicrobial resistance markers detected in step (e), and optionally continuing to culture said clinical sample and/or portion in said first and/or second culture vessel or g) if no microorganism strain is identified in step (e), further culturing said clinical sample and/or portion to enable further microbial identification and antimicrobial susceptibility tests to be performed to identify the microorganism and determine its antimicrobial resistance profile.A device for performing the method of the invention is also provided.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor

Abstract

The present invention provides a method for detecting and characterising a microorganism in aclinical sample, said method comprising: a) introducing a clinical sample to a first culture vessel containing culture medium; b (i) optionally preculturing said clinical sample in said first culture vessel; (ii) optionally removing a portion of the clinical sample/medium mixture or, if precultured, the clinical sample culture from said first culture vessel, and introducing said portion to a second culture vessel containing culture medium, and optionally preculturing said portion in said second culture vessel; c) removing a test aliquot from said first and/or second culture vessel, and culturing or continuing to culture said clinical sample and/or portion in said first and/or second culture vessel; d) separating DNA from said test aliquot; e) performing nucleic acid tests on said DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in said microorganism, wherein said nucleic acid tests are performed using: i) one or more nucleic acid probes and/or primers for microbial identification, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence which is identificatory of a given microorganism; and ii) one or more nucleic acid probes and/or primers for antimicrobial resistance marker detection, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence representing a genetic antimicrobial resistance marker; and it is detected whether or not said probes and/or primers have hybridised to said DNA and/or said primers have been extended (e.g. an amplification reaction has taken place); and f) if a microorganism is identified in step (e), performing an antimicrobial susceptibility test on said cultured clinical sample and/or portion from step (c), wherein microbial growth in said antimicrobial susceptibility test is monitored by assessing growth or markers for growth, and wherein the type and concentration of antimicrobial agents used in said antimicrobial susceptibility test is determined by the identity of the microorganism and antimicrobial resistance markers detected in step (e), and optionally continuing to culture said clinical sample and/or portion in said first and/or second culture vessel or g) if no microorganism strain is identified in step (e), further culturing said clinical sample and/or portion to enable further microbial identification and antimicrobial susceptibility tests to be performed to identify the microorganism and determine its antimicrobial resistance profile.A device for performing the method of the invention is also provided.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6813 - Hybridisation assays
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G02B 21/24 - Base structure
• G02B 21/34 - Microscope slides, e.g. mounting specimens on microscope slides
• G02B 27/40 - Optical focusing aids
• G01N 21/64 - FluorescencePhosphorescence
• B29L 31/00 - Other particular articles
• B29C 45/26 - Moulds

Abstract

A substrate for use in manufacture of a production master plate for production of a detection disc for carrying samples in an apparatus for detection of microscopic objects in a fluid, the substrate having a channel and separate focus structure, wherein the focus structure is a groove.

IPC Classes  ?

• G01N 21/01 - Arrangements or apparatus for facilitating the optical investigation
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• G01N 33/483 - Physical analysis of biological material
• G06M 11/00 - Counting of objects distributed at random, e.g. on a surface

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signalling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes; none of the aforesaid being for use in the design or creation of personalised joint replacement technologies.

Abstract

The present invention is directed to a method for performing a rolling circle amplification (RCA) reaction comprising at least two rounds of RCA, said method comprising: a) providing a concatemeric first RCA product comprising a multiplicity of monomer repeats, each repeat representing a complementary copy of a first RCA template; b) cleaving the first RCA product into monomer units, wherein the monomer unit is reduced in size as compared to the monomer repeat of the first RCA product; c) circularising monomer units resulting from said cleavage to form second RCA templates wherein the second RCA template is smaller than the first RCA template; d) performing a second RCA reaction using said second RCA template of (c) and a primer for said second RCA, to form a second RCA product; wherein any one or more of steps (a) to (d) may be performed sequentially or in combination. The method may be used in the detection and/or analysis of a nucleic acid molecule, including in particular in the detection of an analyte.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes. Analysis in the field of molecular biology; research relating to molecular sciences; technological research; technological studies; technological consultancy; technological services; scientific and technological services; consultancy services relating to technological research; scientific and technological services and research; scientific and technological services and research and development relating thereto; scientific and technological services and research and design services related thereto; consultancy and research services in the fields of science, engineering and information technology; provision of information and data relating to scientific and technological research and development; research and development services in the field of bacteriology, cytology, antibody technology, chemistry, immunology and gene expression systems; scientific research; scientific consultancy; scientific analysis; research (scientific -); scientific research services; laboratory (scientific -) services; scientific testing services; scientific advisory services; scientific laboratory services; compilation of scientific information; preparation of scientific reports; scientific research and development; conducting of scientific studies; conducting of scientific experiments; scientific research relating to bacteriology; scientific research relating to biology; scientific research in the field of genetics and genetic engineering; design and development of diagnostic apparatus; research and development in the field of diagnostic preparations; biotechnology testing; microbiological testing; bacteriological research and testing; analytical services relating to the testing of the bacterial content of liquids. Rental of apparatus and installations in the field of medical technology; professional consultancy in the field of medical technology, medical surgery and orthopaedics; professional consultancy in the field of medical technology, medical surgery and orthopaedics; medical diagnostic services; medical and surgical diagnostic services; medical diagnostic services [testing and analysis]; medical testing; services for the testing of blood; services for the testing of sera; medical and health services relating to dna, genetics and genetic testing; medical testing services relating to the diagnosis and treatment of disease.

Goods & Services

Diagnostic preparations for scientific or research use; diagnostic reagents for scientific or research use; biochemicals, namely, monoclonal antibodies for in vitro scientific or research use; reagents for use in scientific apparatus for chemical or biological analysis; chemical preparations for scientific purposes, other than for medical or veterinary use; diagnostic preparations for scientific or research use other than for medical use; nucleic acids; nucleic acid for use in nucleic acid amplification processes [other than for medical use]; nucleic acid amplification reagents [other than for medical use]; nucleic acid sequencing programs [other than for medical use]; nucleic acid sequences, other than for medical or veterinary purposes; chemicals for use in multiplying nucleic acid amounts [other than medical and veterinary]; diagnostic kits comprising specimen receptors and reagents for testing for selected bacteria; diagnostic preparations for scientific purposes; chemical products for diagnostic use [scientific]; chemical diagnostic reagents for industrial use; diagnostic preparations, other than for medical or veterinary purposes. Nucleic acid sequences for medical and veterinary purposes; diagnostic preparations; medical diagnostic reagents; clinical diagnostic reagents; diagnostic preparations for medical purposes. Scientific apparatus; probes for scientific purposes; scientific apparatus and instruments; scientific, optical, measuring, signalling and checking apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; scientific, nautical, surveying, photographic, cinematographic, optical, weighing, measuring, signaling, checking, life-saving and teaching apparatus and instruments; laboratory instrument for the detection of pathogens and toxins in a biological sample for research use; diagnostic apparatus, not for medical purposes; software; computer systems; detection apparatus; measuring instruments; measuring apparatus; testing apparatus for diagnostic purposes [other than medical]. Diagnostic apparatus for the detection of abnormal prion protein; apparatus for analysing bacteria in biological samples [for medical use]; medical diagnostic instruments; tools for medical diagnostics; diagnostic apparatus for medical purposes; diagnostic, examination, and monitoring equipment; testing instruments for medical diagnostic purposes; testing probes for medical diagnostic purposes; testing apparatus for medical purposes. Analysis in the field of molecular biology; research relating to molecular sciences; technological research; technological studies; technological consultancy; technological services; scientific and technological services; consultancy services relating to technological research; scientific and technological services and research; scientific and technological services and research and development relating thereto; scientific and technological services and research and design services related thereto; consultancy and research services in the fields of science, engineering and information technology; provision of information and data relating to scientific and technological research and development; research and development services in the field of bacteriology, cytology, antibody technology, chemistry, immunology and gene expression systems; scientific research; scientific consultancy; scientific analysis; research (scientific -); scientific research services; laboratory (scientific -) services; scientific testing services; scientific advisory services; scientific laboratory services; compilation of scientific information; preparation of scientific reports; scientific research and development; conducting of scientific studies; conducting of scientific experiments; scientific research relating to bacteriology; scientific research relating to biology; scientific research in the field of genetics and genetic engineering; design and development of diagnostic apparatus; research and development in the field of diagnostic preparations; biotechnology testing; microbiological testing; bacteriological research and testing; analytical services relating to the testing of the bacterial content of liquids. Rental of apparatus and installations in the field of medical technology; professional consultancy in the field of medical technology, medical surgery and orthopaedics; professional consultancy in the field of medical technology, medical surgery and orthopaedics; medical diagnostic services; medical and surgical diagnostic services; medical diagnostic services [testing and analysis]; medical testing; services for the testing of blood; services for the testing of sera; medical and health services relating to dna, genetics and genetic testing; medical testing services relating to the diagnosis and treatment of disease.