A tubing assembly includes first and second tubing segments, and a pressure cup portion formed between the first and second tubing segments. The pressure cup portion has a first open lateral side, and a second closed lateral side including a flat membrane member configured to engage a diaphragm of a pressure transmitter to transmit a pressure of a fluid disposed within the pressure cup portion to said diaphragm. The flat membrane member is integrally formed with the pressure cup portion. A mold for forming the tubing assembly, and a pressure sensing system includes the tubing assembly, a housing portion, and a pressure sensor receivable within the housing to position a diaphragm of the pressure sensor directly adjacent to the flat membrane member of the tubing assembly.
B29C 45/00 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor
F16L 41/00 - Branching pipesJoining pipes to walls
F16L 41/02 - Branch units, e.g. made in one piece, welded, riveted
G01L 19/00 - Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
A tubing assembly includes first and second tubing segments, and a pressure cup portion formed between the first and second tubing segments. The pressure cup portion has a first open lateral side, and a second closed lateral side including a flat membrane member configured to engage a diaphragm of a pressure transmitter to transmit a pressure of a fluid disposed within the pressure cup portion to said diaphragm. The flat membrane member is integrally formed with the pressure cup portion. A mold for forming the tubing assembly, and a pressure sensing system includes the tubing assembly, a housing portion, and a pressure sensor receivable within the housing to position a diaphragm of the pressure sensor directly adjacent to the flat membrane member of the tubing assembly.
G01L 19/00 - Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
3.
UNIVERSAL MODULAR PRESSURE SENSORS AND FITTINGS THEREFOR
A system for monitoring pressure in a bioprocessing system includes a binder connector or industrial connector including a plurality of electrically conductive pins disposed therethrough. A sensor cap has an opening for receiving a distal portion of the binder connector. A sensor spacer has an upper end engageable with a surface of the sensor cap. The sensor spacer has an opening for receiving wire leads coupled to the electrically conductive pina. A sensor has a printed circuit board (PCB) disposed thereon. The PCB includes a plurality of wire solder points for electrically coupling to the wire leads. A sensor insert having a sealing portion receives the sensor therein. A sensor housing has a seat portion for receiving the sealing portion thereon and an upper flange portion for coupling to the sensor cap.
G01L 19/00 - Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
The disclosure is directed to pneumatic actuator assemblies for reduced dead leg. An assembly includes a valve body (104), and a first actuator (101) and a second actuator (102) each coupled to the valve body by a bonnet assembly (112). The bonnet assembly includes a bonnet body (125) having a first end (126) opposite a second end (128), wherein the first end is connected with the valve body, and a nut (130) threadedly connected to the second end of the bonnet body. The bonnet assembly further includes an actuator bonnet (134), wherein the nut partially surrounds the actuator bonnet, and wherein the actuator bonnet is rotatable about a lengthwise axis (LA1, LA2) extending through a center of a stem (124) of a piston (118).
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 27/00 - Construction of housingsUse of materials therefor
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
F16K 31/122 - Operating meansReleasing devices actuated by fluid the fluid acting on a piston
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
F16K 11/22 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members with an actuating member for each valve, e.g. interconnected to form multiple-way valves
The disclosure is directed to pneumatic actuator assemblies for reduced dead leg. In one approach, an assembly may include a valve body, and a first actuator and a second actuator each coupled to the valve body by a bonnet assembly. The bonnet assembly may include a bonnet body having a first end opposite a second end, wherein the first end is connected with the valve body, and a nut threadedly connected to the second end of the bonnet body. The bonnet assembly may further include an actuator bonnet, wherein the nut partially surrounds the actuator bonnet, and wherein the actuator bonnet is rotatable about a lengthwise axis extending through a center of a stem of a piston.
F16K 31/122 - Operating meansReleasing devices actuated by fluid the fluid acting on a piston
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
09 - Scientific and electric apparatus and instruments
Goods & Services
Software used in connection with measuring and analyzing chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use.
The present invention relates to Fc binding proteins comprising one or more Fc binding domains wherein at least one domain comprises of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-6 or 21. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
C07K 14/31 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof
The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.
B01D 65/08 - Prevention of membrane fouling or of concentration polarisation
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.
A pinch valve including a main body having a jaw defining a tube cradle for holding a tube, a clamp pivotably coupled to the jaw and rotatable between an open position and a closed position, the clamp having a shank extending therefrom, wherein when the clamp is in the open position the shank extends into the tube cradle such that when the tube is placed into the tube cradle the tube engages the shank and rotates the clamp toward the closed position, and wherein when the clamp is rotated from the closed position to the open position the shank pushes the tube at least partially out of the tube cradle, and a linear actuator coupled to a rear of the main body and including a plunger that can be controllably driven into the tube cradle for compressing the tube against the clamp to restrict fluid flow through the tube.
A61M 39/28 - Clamping means for squeezing flexible tubes, e.g. roller clamps
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
09 - Scientific and electric apparatus and instruments
Goods & Services
Downloadable software used in connection programing and controlling automated production systems used in the pharmaceutical and biopharmaceutical industry to measure and analyze chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use; embedded software used in connection programing and controlling automated production systems used in the pharmaceutical and biopharmaceutical industry to measure and analyze chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants for scientific and industrial research and use
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
B01D 29/66 - Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
Viral vectors for gene therapy can be produced using transfection in cell culture systems. The present disclosures describe cell culture systems that include cell retention devices and other components so that transfection occurs at high values of viable cell density (VCD). The present disclosures also include methods of operating cell culture systems at high values of viable cell density for optimized production of viral vectors.
The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.
A bioprocessing system includes a process vessel, a plurality of filter housings, a feed pump for moving fluid between the process vessel and the filtration assembly, and a retentate valve within a retentate line. The retentate valve includes a fluid passageway to selectively direct movement of the fluid to at least one of the plurality of filter housings. A permeate valve is provided in a permeate line between the filter housings and a permeate pump. A pressure sensor is provided in the permeate line, and a controller is coupled to the pressure sensor, the retentate valve, and the permeate valve. The controller actuates the retentate and permeate valves to direct flow between the process vessel one or more of the filter housings, and to direct permeate flow from the filter housings to the permeate pump based on information received from the flow sensor and the pressure sensor.
09 - Scientific and electric apparatus and instruments
Goods & Services
Chemical analysis instruments, none of them for medical use, for the analysis of biochemical processes namely for the compositional analysis of liquids employed in bioprocess development and production using enzymatic sensors, electrochemical sensors or a combination thereof; computing devices, data-loggers, data acquisition devices, data storage devices, programmable logic control devices, networking devices and associated embedded software for data management and data analysis in the field of chemical analysis process instrumentation and control, such data management and analysis for operating chemical analytical instruments and communicating results and control signals with other network systems.
21.
GENDERLESS CLAMP AND CLAMP HALF FOR SECURING HYGIENIC FITTINGS
A genderless clamp includes a first clamp portion and a second clamp portion. The first clamp portion has a first end and a second end. The second clamp portion has a first end and a second end. For each clamp portion, the first end has ramped barbs and the second end also has ramped barbs. The first clamp portion mates with the second clamp portion around a junction through which liquid is to flow.
Presented herein are methods and systems for monitoring and/or control of production units used at various stages in development and manufacture of biological products. In particular, in certain embodiments, bio-production monitoring and control technologies described herein utilize mid-infrared (mid-IR) analyzers capable of obtaining mid-IR spectral data from aqueous samples in substantially real-time. Technologies described herein may leverage this mid-IR spectral data to measure sample quality metrics, such as protein content, titer, secondary structure, aggregation, etc. as well as viral and/or nucleic acid properties. Sample quality metrics may, accordingly, be measured in substantially real-time and/or on a continuous basis to assess production quality for, e.g., protein therapeutics and gene-therapy agents, and may be used by control systems for adjustment and/or control of process parameters, thereby improving, among other things, product quality.
The present disclosure relates to diaphragm pumps with one or more filters, e.g., tangential flow filters, membranes, and ultrafiltration membranes, for various applications, including, e.g., bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same. In an aspect, a pump for alternating tangential flow filtration may include a diaphragm. A housing may contain the diaphragm. The housing may have a first end open to a retentate channel of a first membrane. A magnetic member may be coupled to the diaphragm. An electromagnetic field generator may be disposed about the magnetic member.
F04B 43/02 - Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
F04B 45/04 - Pumps or pumping installations having flexible working members and specially adapted for elastic fluids having plate-like flexible members, e.g. diaphragms
A valve assembly with a multidirectional flow path includes a valve body that includes respective hinged portions defining respective passageways extending through the valve body when in a closed state. A flexible conduit or tubing having a loop portion and a plurality of branches extending from the loop portion is at least partially positioned within the valve body. Portions of the loop that extend beyond the valve body are encapsulated by respective rigid jackets. Multiple valves are situated on the valve body and are used to selectively pinch the loop portion in different pinching configurations to direct fluid to or from the particular branches. The valve assembly may be integrated into a manufacturing or other production process with optional additional jackets used to encapsulate the flexible conduit branches.
F16K 11/22 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit operated by separate actuating members with an actuating member for each valve, e.g. interconnected to form multiple-way valves
B29C 45/14 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
B29C 65/00 - Joining of preformed partsApparatus therefor
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
F16K 27/00 - Construction of housingsUse of materials therefor
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
A fluid filtration system includes a fluid storage vessel such as a bioreactor, one or more filters housings, and at least one pump coupled between the fluid storage vessel and the filter housing(s). A plurality of valves are used to control flow between the pump and the filter housing. The at least one pump is configured to move fluid from the fluid storage vessel through the filter housing(s), and the valves are configured for selectively directing fluid received from the at least one pump to the first or second end of the filter housing(s). A plunger pump can be used to selectively move flow between first and second ends of a filter housing.
The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C07K 14/31 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
28.
ENHANCED BIOPROCESSING FILM FOR SINGLE USE BIOCONTAINERS
A multilayer polymer film comprising an inner biomaterial contact layer and an outer layer, the outer layer comprising a polymer selected from a polyester elastomer copolymer, polyethylene terephthalate (PET), a nylon, polyethylene, polypropylene, a thermoplastic elastomer, and copolymers and mixtures thereof, wherein the melting temperature of the inner contact layer and the outer layer differ by 30-120 °C.
A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01J 3/10 - Arrangements of light sources specially adapted for spectrometry or colorimetry
30.
RECOVERY OF PERFUSION BLEED PRODUCT VIA ALTERNATING TANGENTIAL FLOW FILTRATION IN A SEDIMENTATION REACTOR
A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.
A bleed recovery system for use in a bioreactor system is disclosed. The bleed recovery system being configured to recover product of interest from the bleed material of the bioreactor system. In one embodiment, the bioreactor system includes a bleed recovery system for removing cell cultures from the bioreactor. The bleed recovery system includes a bleed pump coupled to the bioreactor so that cell bleed material can be removed from the bioreactor, a bleed vessel for receiving the cell bleed material, a cell retention device coupled to the bleed vessel, a harvest pump coupled to the cell retention device to deposit the product of interest into a second harvest tank, and a bleed pump connected to the bleed vessel to deposit the spent bleed material into a waste tank. Thus arranged, some or all of the product of interest can be separated from the spent medium.
A system may include a light source, to generate a probe signal, comprising incident radiation; an optical probe, to direct the incident radiation through a fluid sample; a motor, to move the optical probe along a probe axis, to change a path length of the incident radiation through the fluid sample; and a detector, to receive the incident radiation as attenuated radiation after passing through the fluid sample. The system may include a control system, arranged to: initiate an absorbance measurement by directing movement of the optical probe along the probe axis; direct the light source to emit the incident radiation; and automatically adjust at least one measurement parameter of a set of measurement parameters for the sample measurement, based upon a slope parameter m, wherein m is derived from a rate of change in an intensity of the attenuated radiation with a change in the path length.
G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
34.
COMPACT HIGH RESOLUTION MONOCHROMATIC LIGHT SOURCE FOR FLUID SAMPLE CONCENTRATION MEASUREMENT
A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/85 - Investigating moving fluids or granular solids
G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
G01J 3/10 - Arrangements of light sources specially adapted for spectrometry or colorimetry
G01N 21/25 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
36.
MULTI-MONOCHROMATIC LIGHT SOURCE SYSTEM FOR SLOPE SPECTROSCOPY
An apparatus may include a light emitting diode (LED) assembly, comprising a plurality of LEDs that output radiation at a plurality of different wavelengths, respectively. The LED assembly may be arranged to output a composite probe signal at a plurality of instances, wherein the composite probe signal comprises a plurality of probe signals, generated from the plurality of LEDs, respectively. The apparatus may also include a measurement module that includes an optic probe to direct the composite probe signal through a fluid sample, while moving between a plurality of probe positions at the plurality of instances. The measurement module may include a detector, disposed to detect, at the plurality of instances, a transmitted intensity of the composite probe signal at the plurality of different wavelengths after the composite probe signal passes through the fluid sample.
G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
G01N 21/25 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/85 - Investigating moving fluids or granular solids
38.
VARIABLE PATH LENGTH ABSORPTION SPECTROMETER HAVING AUTOMATED CONTINUOUS SLOPE MEASUREMENT
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/85 - Investigating moving fluids or granular solids
39.
MULTIMONOCHROMATIC LIGHT SOURCE SYSTEM FOR SLOPE SPECTROSCOPY
An apparatus may include a light emitting diode (LED) assembly, comprising a plurality of LEDs that output radiation at a plurality of different wavelengths, respectively. The LED assembly may be arranged to output a composite probe signal at a plurality of instances, wherein the composite probe signal comprises a plurality of probe signals, generated from the plurality of LEDs, respectively. The apparatus may also include a measurement module that includes an optic probe to direct the composite probe signal through a fluid sample, while moving between a plurality of probe positions at the plurality of instances. The measurement module may include a detector, disposed to detect, at the plurality of instances, a transmitted intensity of the composite probe signal at the plurality of different wavelengths after the composite probe signal passes through the fluid sample.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/85 - Investigating moving fluids or granular solids
40.
Encapsulated system for pressurized fluid processes
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
F16L 57/00 - Protection of pipes or objects of similar shape against external or internal damage or wear
F16L 57/02 - Protection of pipes or objects of similar shape against external or internal damage or wear against cracking or buckling
If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
The present invention relates to Fc binding proteins comprising one or more domains with Cysteine in the C-terminal helical region. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.
A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
G01N 15/06 - Investigating concentration of particle suspensions
45.
FILTRATION UNITS AND ASSEMBLY, AND METHODS OF MAKING AND USING SAME
A self-contained filtration assembly with a self-contained filtration cassette therein. The cassette may be formed from one or more layers membranes and spacers (spacers, screens, or combinations thereof) all having the same general shape. The cassette may have a feed inlet path and a retentate outlet path extending through the layers of the cassette within the perimeter of the cassette, and a permeate outlet path between the cassette exterior and the interior of the cassette housing. The housing may have a generally circular or elliptical cross-section. A clamping assembly may hold together the components of the housing, and may be elongated in a direction along the flow path of the feedstream through the cassette. Various port-defining zones defined in the layers of the cassette may be sealed in a manner facilitating assembly of the cassette as well as isolation of the flow paths therein.
A self-contained filtration assembly with a self-contained filtration cassette therein. The cassette may be formed from one or more layers membranes and spacers (spacers, screens, or combinations thereof) all having the same general shape. The cassette may have a feed inlet path and a retentate outlet path extending through the layers of the cassette within the perimeter of the cassette, and a permeate outlet path between the cassette exterior and the interior of the cassette housing. The housing may have a generally circular or elliptical cross-section. A clamping assembly may hold together the components of the housing, and may be elongated in a direction along the flow path of the feedstream through the cassette. Various port-defining zones defined in the layers of the cassette may be sealed in a manner facilitating assembly of the cassette as well as isolation of the flow paths therein.
A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.
A method of improving volumetric productivity from a cell culture includes filtering a cell culture through an ultrafilter or a microfilter operating in tangential flow filtration mode or alternating flow filtration mode, and filtering the cell culture concurrently or intermittently through a tangential flow depth filtration system to remove cellular debris and/or to harvest cell product. A system for filtering biological materials includes a primary filtration system, and a secondary filtration system, where the secondary filtration system comprises a tangential flow depth filtration filter.
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
Vessel configured to facilitate concentration of a product in a solution. The vessel may include outlet ports to deliver the solution to a processing system (such as a filtration system), and inlet ports. The inlet ports may be at progressively lower levels for return of fluid thereto reduced in volume as a result of filtration thereof. A smaller volume sump may be provided below a main tank of the vessel to facilitate concentration of the solution. The sump may include ridges facilitating collection of the product of interest from the solution, and/or reinforcing the sump walls, and optionally forming a vortex breaker. A spray ball may be provided to spray materials, such as buffer solution, into the vessel. The spray ball may direct materials to facilitate retrieval of product from the vessel. One or more components of the vessel may be advantageously formed of disposable material for single use thereof.
Vessel configured to facilitate concentration of a product in a solution. The vessel may include outlet ports to deliver the solution to a processing system (such as a filtration system), and inlet ports. The inlet ports may be at progressively lower levels for return of fluid thereto reduced in volume as a result of filtration thereof. A smaller volume sump may be provided below a main tank of the vessel to facilitate concentration of the solution. The sump may include ridges facilitating collection of the product of interest from the solution, and/or reinforcing the sump walls, and optionally forming a vortex breaker. A spray ball may be provided to spray materials, such as buffer solution, into the vessel. The spray ball may direct materials to facilitate retrieval of product from the vessel. One or more components of the vessel may be advantageously formed of disposable material for single use thereof.
B01F 27/85 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with two or more stirrers on separate shafts
52.
TUBING COUPLER MOLDINGS AND SYSTEMS, AND ASSOCIATED METHODS
A tubing system with a coupler join positioned between and joining tubing segments. The coupler join is formed to reduce dead legs and hold up volumes within, and/or to reduce materials used to form, and/or to simplify the manufacturing process of the tubing system. The coupler join may be sized, shaped, configured, and/or dimensioned to join tubing segments, such as pre-formed tubing segments, in a streamlined configuration, such as to substantially correspond with the outer and inner diameters of the tubing segments. The tubing system and coupler join may be formed with a mold configured to form a coupler join securely coupling spaced apart ends of tubing segments to be joined together in a configuration which reduces dead legs and hold up volumes within the resulting tubing system.
B29C 45/14 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
B29C 65/70 - Joining of preformed partsApparatus therefor by moulding
B29C 65/00 - Joining of preformed partsApparatus therefor
A tubing system with a coupler join positioned between and joining tubing segments. The coupler join is formed to reduce dead legs and hold up volumes within, and/or to reduce materials used to form, and/or to simplify the manufacturing process of the tubing system. The coupler join may be sized, shaped, configured, and/or dimensioned to join tubing segments, such as pre-formed tubing segments, in a streamlined configuration, such as to substantially correspond with the outer and inner diameters of the tubing segments. The tubing system and coupler join may be formed with a mold configured to form a coupler join securely coupling spaced apart ends of tubing segments to be joined together in a configuration which reduces dead legs and hold up volumes within the resulting tubing system.
B29C 45/14 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory consumables kits for use with cell culture media analyzers comprised primarily of microfluidic chips for cell culture analysis, laboratory microplates, and covers for laboratory microplates, and also consisting of background electrolytes for cell culture analysis, and diluent for cell culture analysis
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
Laboratory consumables kits for use with cell culture media analyzers comprised primarily of chemicals in the nature of standard solutions used in analytical chemistry
A filter screening tool, system, and method allowing high throughput screening of filters (such as hollow fiber filters), such as for use in bioprocessing workflows. Feed material may be fed through different filter modules to assess, measure, analyze, determine, evaluate, teste, compare, screen, etc., properties or characteristics, etc., of filters within the filter modules. Each filter module may house a different type (e.g., material or configuration) of filter so that different filters may be compared and screened, such as for a selected use.
The present disclosure is related to bioprocessing devices, systems, and methods, and particularly to sanitary connections for bioprocessing. The devices, systems, and methods described herein can relate to the sanitary connection of flow fittings, such as reducers, with considerations for ease of assembly and sealing. In some embodiments, the system may include a flow reducer, a ferrule and a gasket. The flow reducer may include a gasket channel, an overmold channel within the gasket channel, and one or more cavities within the overmold channel. The gasket may be overmolded within the gasket channel and extending within the one or more cavities.
F16L 25/14 - Joints for pipes of different diameters or cross-section
F16L 21/03 - Joints with sleeve or socket with elastic sealing rings between pipe and sleeve or between pipe and socket, e.g. with rolling or other prefabricated profiled rings placed in the socket before connection
09 - Scientific and electric apparatus and instruments
Goods & Services
Detection and identification instruments using light sources and light detectors for detecting, identifying, and quantifying chemical and biological substances not for medical purposes; Scientific apparatus, namely, spectrometers and parts, fittings, probes, fiberoptic cables thereof.
60.
ALTERNATING TANGENTIAL FLOW BIOREACTOR WITH HOLLOW FIBER SYSTEM AND METHOD OF USE
Embodiments of the present disclosure relate generally to systems and methods for perfusion cell culture involving alternating fluid flows between first and second flexible vessels. For example, a hollow fiber filter module may be attached to first and second culture vessels which each include inner and outer vessels. A pressure source may cause a pressure differential between the outer vessels, which may cause a responsive fluid flow between the inner vessels across a hollow fiber filtration unit.
A pressure gauge and/or relief valve (100) which may be used in a single-use fluid system, such as a sterilizable fluid system. The pressure gauge and/or pressure relief valve may be formed of a disposable and/or sterilizable material, such as a non-metallic (e.g., polymeric) material. The material of the pressure gauge and/or pressure relief valve may be compatible with the material of a fluid-containing structure of the fluid system, such as to be bonded (e.g., welded) therewith. The fluid-containing structure may be retrofitted to integrate the pressure gauge and/or pressure relief valve therewith. The pressure gauge and/or pressure relief valve may have a simple configuration, such as a housing (120) with a movable element (130) therein. The movable element moves with respect to the housing to indicate pressure and/or to relieve pressure from within the housing and/or fluid-containing structure.
F16K 17/04 - Safety valvesEqualising valves opening on surplus pressure on one sideSafety valvesEqualising valves closing on insufficient pressure on one side spring-loaded
F16K 37/00 - Special means in or on valves or other cut-off apparatus for indicating or recording operation thereof, or for enabling an alarm to be given
A61M 5/168 - Means for controlling media flow to the body or for metering media to the body, e.g. drip meters, counters
G01L 19/06 - Means for preventing overload or deleterious influence of the measured medium on the measuring device or vice versa
62.
Pressure gauge relief valve for sterilizable fluid system
A pressure gauge and/or relief valve which may be used in a single-use fluid system, such as a sterilizable fluid system. The pressure gauge and/or pressure relief valve may be configured to measure and to relieve fluid within a fluid-containing structure, the pressure gauge relief valve including a housing having a first end, a second end, and an inner surface defining a passage between the first end and the second end, a movable element movable within the passage, wherein the housing first end is configured to be coupled with a fluid-containing structure, the housing second end is spaced from the housing first end; and the movable element is movable with respect to the housing to indicate pressure therein and to allow fluid to pass between the movable element and the inner surface of the passage at a selected pressure.
G01L 19/00 - Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
G01L 19/06 - Means for preventing overload or deleterious influence of the measured medium on the measuring device or vice versa
F16K 17/04 - Safety valvesEqualising valves opening on surplus pressure on one sideSafety valvesEqualising valves closing on insufficient pressure on one side spring-loaded
A fluid filtration assembly includes a filter housing having first and second ends and a connector for fluid communication with a fluid storage vessel. A filter element is disposable within the filter housing, and first and second pumps are coupled at the first and second ends of the filter housing. A controller may coordinate the operation of the first and second pumps to induce alternating tangential flow of fluid between the filter housing and the first and second pumps. At least one of the first and second pumps is a diaphragm pump or a plunger pump. The fluid storage vessel can be a bioreactor.
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
An encapsulated valve system includes a first housing portion having a first facing surface, the first facing surface comprising a plurality of branch pathways formed as a recess within the first facing surface. The valve system further includes a second housing portion having a second facing surface, the second facing surface comprising a plurality of branch pathways formed as a recess within the second facing surface. A disposable conduit is configured to be interposed between the first and second housing portions and disposed within the recess of the first facing surface and the recess of the second facing surface. The disposable conduit is thus sandwiched between the first and second facing surfaces. A plurality of pinch valve actuators are mounted on one or both of the first housing portion and the second housing portion, the plurality of pinch valve actuators configured to pinch the disposable conduit at selective branch pathways.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
F16K 27/00 - Construction of housingsUse of materials therefor
F16K 7/00 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage
F16K 31/06 - Operating meansReleasing devices electricOperating meansReleasing devices magnetic using a magnet
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 11/02 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit
F16K 11/10 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit
F16K 3/30 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing Details
66.
Encapsulated system for pressurized fluid processes
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16L 57/00 - Protection of pipes or objects of similar shape against external or internal damage or wear
F16L 57/02 - Protection of pipes or objects of similar shape against external or internal damage or wear against cracking or buckling
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
67.
Plunger pumping arrangement for a hollow fiber filter
A fluid filtration assembly includes a filter housing having a first end for fluid connection with a fluid storage vessel. A filter cartridge is disposable within the filter housing, and a plunger pump is coupled at a second end of the filter housing. The plunger pump includes a housing having a rigid portion and a flexible portion. The flexible portion has a plunger-engaging portion for coupling to the plunger of an actuator. The flexible portion selectively movable with respect to the rigid portion via the actuator. The filter cartridge can be a hollow fiber filter. The plunger pump can be configured to induce alternating tangential flow in at least a portion of the assembly. The fluid filtration assembly can be provided as a disposable single-use arrangement.
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
F04B 43/00 - Machines, pumps, or pumping installations having flexible working members
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific instruments, namely, electronic monitors comprised of software and hardware for measuring and analyzing in real time chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants.
09 - Scientific and electric apparatus and instruments
Goods & Services
(1) Scientific instruments, namely, electronic monitors comprised of software and hardware for measuring and analyzing in real time chemical and biological substances for the presence, absence, and/or quantity of target chemicals, biologics, and/or contaminants
C12N 15/64 - General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
B01D 61/00 - Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltrationApparatus, accessories or auxiliary operations specially adapted therefor
B01D 29/60 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor integrally combined with devices for controlling the filtration
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/00 - Apparatus for enzymology or microbiology
73.
Filtration system and method for synchronizing permeate and retentate flow
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
B01D 29/66 - Regenerating the filter material in the filter by flushing, e.g. counter-current air-bumps
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
B01D 29/00 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor
B01D 29/11 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor with bag, cage, hose, tube, sleeve or like filtering elements
A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.
The present disclosure relates to hollow fiber tangential flow filter units, including hollow fiber tangential flow depth filter units, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.
A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.
F04B 49/20 - Control of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for in, or of interest apart from, groups by changing the driving speed
F04B 43/00 - Machines, pumps, or pumping installations having flexible working members
Fluid management systems for handling pressurized fluid connect various subsystems or subunits without the need to use reinforced tubing. The system utilizes one or more segments of unreinforced conduit that are encapsulated at various points along a length of the segment with one or more rigid encapsulating members. The unreinforced conduit may be made a disposable element while the rigid encapsulating members may be re-used. In one aspect, the encapsulating member may include a two-part valve body that surrounds and encapsulates a portion of the unreinforced conduit. In another aspect, the encapsulating member may include a two-part jacket that surrounds and encapsulates a portion of the unreinforced conduit. The two-part valve bodies and two-part jackets may be joined at various points within the system as part of the overall flow system.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16L 57/02 - Protection of pipes or objects of similar shape against external or internal damage or wear against cracking or buckling
F16K 27/02 - Construction of housingsUse of materials therefor of lift valves
F16L 57/00 - Protection of pipes or objects of similar shape against external or internal damage or wear
80.
Methods and systems for removing pressure and air from chromatography columns
Methods and systems for removing gases and/or pressure formed during the sterilization, e.g., the gamma irradiation, of prepacked chromatography systems (column plus attached tube and valve set) are described. The methods include purging the gas and/or pressure through specially designed tube and valve sets without breaching the sterility of the prepacked sterile chromatography system. The systems include a sterile or aseptic pre-packed chromatography column including a column having an inlet and an outlet, a tubing and valve set attached to the inlet and the outlet, and a pump configured to pump sterile or aseptic liquid from the fluid source along the tubing and valve set, into the column tube inlet and out of the column tube outlet along a first flow path, thereby removing any entrapped gas and/or pressure from the chamber.
B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
A method for promoting biological activity uses a filter system to increase cell production of a fed batch bioreactor. The filter system cycles bioreactor fluid through a hollow fiber tangential flow filter which separates metabolic wastes (as well as proteins) from cells produced in bioreactor and returned to fed batch bioreactor, improving cell production in the fed batch bioreactor. The filter system includes a disposable pump and filter, and a reusable control system. The pump is a low shear gamma stable pump gently cycling bioreactor fluid through the filter with minimal damage to the cells produced in the bioreactor. The pumphead and hollow fiber tangential flow filter are disposable. The pump motor is part of the control system and is reusable. The pumphead and filter are provided as an assembled and pre-sterilized unit allowing simple and quick attachment to the fed batch bioreactor, and simple and quick disposal.
09 - Scientific and electric apparatus and instruments
Goods & Services
Analytical instruments not for medical use for the compositional analysis of liquids employed in bioprocess development and production using chemical sensors; downloadable computer software for operating the foregoing analytical instruments and communicating results and control signals with other systems; computer hardware with embedded software for operating the foregoing analytical instruments and communicating results and control signals with other systems
83.
Method of actuation of an alternating tangential flow diaphragm pump
A control unit includes a first connector configured to connect a proportional pressure regulator to a positive pressure supply and a second connector configured to connect the proportional pressure regulator to a negative pressure supply. The control unit further includes at least one sensor configured to detect an amount of air flow (volume per unit of time), positive or negative, within an air flow line connected to an output of the proportional pressure regulator, and a third connector configured to connect the air flow line to an air side of a diaphragm. Additionally, the control unit includes a controller programmed to control at least an opening and closing function of the proportional pressure regulator to attain a desired amount of air flow (volume per unit of time), positive or negative, within the air flow line.
F04B 49/20 - Control of, or safety measures for, machines, pumps, or pumping installations, not otherwise provided for in, or of interest apart from, groups by changing the driving speed
The present disclosure relates to spectroscopy with light emitting components including, e.g., UV and/or visible wavelength light, for various applications, including, e.g., chromatography, and more particularly, for a sampling device that facilitates spectroscopic measurements with a variable pathlength and methods for such a device. In an aspect, a device for measuring light absorbance of a sample may include a fluid conduit comprising a first portion, a midportion substantially perpendicular to the first portion, and a second portion substantially perpendicular to the midportion. A first probe may be within the midportion and substantially parallel with the midportion. The first probe may comprise a distal end. A light source may be operably coupled to the first probe. A detector may be aligned with the distal end of the first probe substantially perpendicular to the first probe at a pathlength from the distal end of the first probe.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
A micro alternating tangential flow (microATF) perfusion filter includes a hollow cylinder having a proximal end and a distal end. The proximal end is connected in series to a permeate chamber, followed by a retentate chamber. The proximal end either (i) terminates in or at the permeate chamber, or (ii) terminates in or at the retentate chamber. The portion of the proximal end within the permeate chamber, in a case of (ii), possesses at least one opening allowing fluid communication between an inside of the hollow cylinder and the permeate chamber. The microATF perfusion filter further includes an inlet, positioned over the retentate chamber, for communication with a source of positive or negative pressure, and an outlet, positioned in a wall of the permeate chamber, which can be connected to a check valve, which, in turn, can be connected to a hydrophobic fluid vent filter.
09 - Scientific and electric apparatus and instruments
Goods & Services
Detection and identification instruments using light sources and light detectors for detecting, identifying, and quantifying chemical and biological substances not for medical purposes; Scientific apparatus, namely, spectrometers and parts, fittings, probes, fiberoptic cables thereof
87.
Tangential flow depth filtration systems and methods of filtration using same
The present disclosure relates to hollow fiber tangential flow filters, including hollow fiber tangential flow depth filters, for various applications, including bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same.
Devices, systems, and methods described herein relate to determining a concentration of a species of interest in a sample by using a spectrometer. For example, a concentration of a species of interest may be determined by passing a first feed of a sample with a species of interest through a flow-through variable pathlength spectrophotometer and reading a first absorbance value. A change in the concentration of the species of interest may be effected in the sample, and a second feed of the sample may be passed through a flow through variable pathlength spectrophotometer. A second absorbance value may be read. The difference between the first absorbance value and the second absorbance value may be used to determine the concentration of the species of interest.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
e.g.e.g., bioprocessing and pharmaceutical applications, systems employing such filters, and methods of filtration using the same. In an aspect, a pump for alternating tangential flow filtration may include a diaphragm. A housing may contain the diaphragm. The housing may have a first end open to a retentate channel of a first membrane. A magnetic member may be coupled to the diaphragm. An electromagnetic field generator may be disposed about the magnetic member.
F04B 43/02 - Machines, pumps, or pumping installations having flexible working members having plate-like flexible members, e.g. diaphragms
F04B 43/00 - Machines, pumps, or pumping installations having flexible working members
F04B 45/04 - Pumps or pumping installations having flexible working members and specially adapted for elastic fluids having plate-like flexible members, e.g. diaphragms
A61M 1/16 - Dialysis systemsArtificial kidneysBlood oxygenators with membranes
A61M 1/00 - Suction or pumping devices for medical purposesDevices for carrying-off, for treatment of, or for carrying-over, body-liquidsDrainage systems
90.
VENTING OR DEGASSING OF FILTER DEVICES AND FILTRATION SYSTEMS
Devices, systems, and methods for continually degassing / venting a feedstream within a filtration system. The filtration system may include a filter device utilizing hollow fiber filters to filter the feedstream. Additional venting hollow fiber filters vent gas from the filtration system to outside the filtration system. The venting hollow fiber filters may have ends which expose the interior lumens thereof to outside the filter unit to vent gases which pass through the walls of the hollow fiber filters. Degassed permeate thus flows through the filtering hollow fiber filters.
B01D 36/00 - Filter circuits or combinations of filters with other separating devices
B01D 53/22 - Separation of gases or vapoursRecovering vapours of volatile solvents from gasesChemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases or aerosols by diffusion
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific instruments, namely, electronic analyzers for testing and analyzing chemical and biological substances in real time for the presence, absence, or quantity of target chemicals, biologics, and contaminants
The present disclosure relates generally to alternating tangential flow (ATF) perfusion pumping methods, including novel methods for generating positive and negative pressure at the point of use, and more particularly, to apparatuses, systems and methods for use of the same.
B01D 29/90 - Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups Filtering elements therefor having feed or discharge devices for feeding
An encapsulated valve system includes a first housing portion having a first facing surface, the first facing surface comprising a plurality of branch pathways formed as a recess within the first facing surface. The valve system further includes a second housing portion having a second facing surface, the second facing surface comprising a plurality of branch pathways formed as a recess within the second facing surface. A disposable conduit is configured to be interposed between the first and second housing portions and disposed within the recess of the first facing surface and the recess of the second facing surface. The disposable conduit is thus sandwiched between the first and second facing surfaces. A plurality of pinch valve actuators are mounted on one or both of the first housing portion and the second housing portion, the plurality of pinch valve actuators configured to pinch the disposable conduit at selective branch pathways.
F16K 7/04 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force
F16K 3/30 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing Details
F16K 7/00 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage
F16K 7/06 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of a screw-spindle, cam, or other mechanical means
F16K 7/07 - Diaphragm cut-off apparatus, e.g. with a member deformed, but not moved bodily, to close the passage with tubular diaphragm constrictable by external radial force by means of fluid pressure
F16K 11/02 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit
F16K 11/10 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with two or more closure members not moving as a unit
F16K 27/00 - Construction of housingsUse of materials therefor
F16K 31/06 - Operating meansReleasing devices electricOperating meansReleasing devices magnetic using a magnet
95.
METHOD OF USING TRACK ETCHED MEMBRANES FOR THE FILTRATION OF BIOLOGICAL FLUIDS
C12M 1/00 - Apparatus for enzymology or microbiology
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
B32B 7/12 - Interconnection of layers using interposed adhesives or interposed materials with bonding properties
B32B 27/08 - Layered products essentially comprising synthetic resin as the main or only constituent of a layer next to another layer of a specific substance of synthetic resin of a different kind
F16L 13/11 - Adhesive or cemented joints using materials which fill the space between parts of a joint before hardening
The present disclosure provides apparatuses and methods that integrate sensors into bioprocessing systems and, advantageously, do not require significant re-engineering of these systems. The disclosure describes an assembly for alternating tangential flow filtration. The assembly also includes a sensor inserted into the first hemisphere of the pump housing via the port. In various embodiments, the port comprises a tri-clover connector, and the sensor is optionally inserted into the port through a plug sized to substantially occlude the port and to place the sensor in contact with a fluid in the pump housing.
C12M 1/00 - Apparatus for enzymology or microbiology
F04B 45/04 - Pumps or pumping installations having flexible working members and specially adapted for elastic fluids having plate-like flexible members, e.g. diaphragms
F04B 53/16 - CasingsCylindersCylinder liners or headsFluid connections
The disclosure provides tangential flow depth filtration (TFDF) systems which exhibit improved filter fluxes and process capacities and reduced fouling characteristics. The TFDF systems of the disclosure optionally utilize tubular depth filters (TDF). Methods are provided which include the passage of a non-laminar flow through at least a portion of a length of a TDF in a TFDF system.
A dual path heat exchanger integrated as a single unit able to process fluid stream through a continuous, single-use high temperature short time process. The heat exchanger contains both a heating section and a cooling section in the same unit. In one embodiment, the heating and cooling sections (which may be formed separately for other uses) are formed as a plate and frame structure with a thermally conductive thin film or foil forming the physical barrier between the process stream flow path and the thermal medium (heating or cooling) flow path. The film/foil renders the heat exchanger suitable for single-use and/or to be disposable. A manifold, which also may be formed as a single unit, may be used to transfer fluid flow between the sections of the heat exchanger, and/or to transfer fluid into and out of the system formed by the heat exchanger and manifold.
F28F 3/08 - Elements constructed for building-up into stacks, e.g. capable of being taken apart for cleaning
F28D 9/00 - Heat-exchange apparatus having stationary plate-like or laminated conduit assemblies for both heat-exchange media, the media being in contact with different sides of a conduit wall
F28F 3/02 - Elements or assemblies thereof with means for increasing heat-transfer area, e.g. with fins, with recesses, with corrugations
99.
HIGH-TEMPERATURE SHORT-TIME TREATMENT DEVICE, SYSTEM, AND METHOD
A dual path heat exchanger integrated as a single unit able to process fluid stream through a continuous, single-use high temperature short time process. The heat exchanger contains both a heating section and a cooling section in the same unit. In one embodiment, the heating and cooling sections (which may be formed separately for other uses) are formed as a plate and frame structure with a thermally conductive thin film or foil forming the physical barrier between the process stream flow path and the thermal medium (heating or cooling) flow path. The film/foil renders the heat exchanger suitable for single-use and/or to be disposable. A manifold, which also may be formed as a single unit, may be used to transfer fluid flow between the sections of the heat exchanger, and/or to transfer fluid into and out of the system formed by the heat exchanger and manifold.
F28D 9/00 - Heat-exchange apparatus having stationary plate-like or laminated conduit assemblies for both heat-exchange media, the media being in contact with different sides of a conduit wall
A system for determining a characteristic of a sample includes a light source for directing light into an input of a spectrometer. The spectrometer splits the received light into light outputs each having a different wavelength. An active wavelength selection module (AWSM) includes an optical receiving component (ORC). An actuator is coupled to the spectrometer and/or the ORC to adjust a relative position between the spectrometer and the AWSM so that light is receivable by the ORC from a selected one of the plurality of light outputs. The ORC is configured to direct the received light to a sample. A collector is positioned to collect a portion of light that passes through the sample, and to deliver the collected light to an analysis module. The analysis module is configured to determine a quantity of light transmitted through the sample and to correlate transmitted light with a characteristic of the sample.
