Abstract

Provided are a method and a living cell freezing system for freezing an unfrozen specimen comprising living cells in such a manner as to keep the cells alive. The method comprises: causing an unfrozen specimen to reach a frozen state by placing the unfrozen specimen, that includes a freezing solution containing a freezing protection agent and living cells immersed in the freezing solution, in an electrical field, generated by an electrode to which an alternating current with a frequency of 10-200 Hz is applied, so that said specimen is cooled while conduction electrical current flows. The electrical field is continuously generated without any interruption for a duration that the specimen goes from an unfrozen state to a frozen state. Also provided is a method for forming spheroids, the method comprising: freezing living cells having a spheroid-forming function by the foregoing freezing method; thawing the frozen living cells; and cultivating the thawed living cells to form spheroids.

IPC Classes  ?

• C12N 1/04 - Preserving or maintaining viable microorganisms
• C12N 5/07 - Animal cells or tissues
• C12N 5/0735 - Embryonic stem cellsEmbryonic germ cells
• F25B 21/00 - Machines, plants or systems, using electric or magnetic effects

Abstract

Provided are a live cell freezing system and a method for freezing live cells that includes cooling an unfrozen sample that contains a freezing solution containing a cryoprotectant and live cells that are immersed in the freezing solution while allowing a conduction current to flow by placing the sample in an electric field generated from an electrode to which an alternating current voltage has been applied to freeze the unfrozen sample. Also provide is a method for forming spheroids that includes freezing live cells having spheroid formation ability by the freezing method, thawing the frozen live cells, and culturing the thawed live cells to form spheroids.

IPC Classes  ?

• C12N 1/04 - Preserving or maintaining viable microorganisms
• C12N 5/07 - Animal cells or tissues
• C12N 5/0735 - Embryonic stem cellsEmbryonic germ cells
• F25B 21/00 - Machines, plants or systems, using electric or magnetic effects

Abstract

This defrosting device comprises two or more blowers in which the rotation speed of blades can be controlled, a heater arranged at the location of an airflow produced by the blowers, and a casing that accommodates the blowers and the heater. A frozen food product arranged near the casing is defrosted through heat exchange with air warmed by the heat of the heater.

IPC Classes  ?

• A23L 3/365 - Thawing subsequent to freezing
• F25D 23/12 - Arrangements of compartments additional to cooling compartmentsCombinations of refrigerators with other equipment, e.g. stove

Abstract

The purpose of the present invention is to provide a method for preserving the freshness of an edible meat over a long period of time, said method comprising sufficiently discharging blood from body at a bleeding step in meat processing.　A method for preserving the freshness of an edible meat, said method comprising perfusing the blood vessels of a bird or animal with a fluid to thereby discharge the blood from the blood vessels and then preserving the edible meat obtained from the bird or animal at a non-freezing temperature of 10°C or lower.

IPC Classes  ?

• A22C 17/00 - Other devices for processing meat or bones
• A22C 21/00 - Processing poultry
• A23B 4/00 - Preservation of meat, sausages, fish or fish products
• A23B 4/06 - FreezingSubsequent thawingCooling

Abstract

A method of thawing an object (21) in a frozen state placed in an interior of a container (10), in which an AC voltage which is 10V or higher and 5 kV or lower is applied to the object (21) in the frozen state to flow a current which is 1 μA or higher and 1000 mA or lower, through the object (21) in the frozen state, includes the steps of: partially thawing the object (21) in the frozen state in such a manner that a temperature in the interior of the container (10) is controlled to cause the object (21) in the frozen state to be partially thawed while applying the AC voltage to the object (21) in the frozen state; and stopping application of the AC voltage to the object (21) and preserving the object (21) while maintaining a state in which the object (21) partially thawed.

IPC Classes  ?

• A23L 3/365 - Thawing subsequent to freezing
• A23B 4/07 - Thawing subsequent to freezing

Abstract

　This thawing method for frozen goods (21) is a method for thawing frozen goods (21) within a container (10), through the application of AC voltage of 10 V to 5 kV, inclusive, to the frozen goods (21), in such a way that an electrical current of 1 μA to 1,000 mA, inclusive, flows to the frozen goods (21), the method being provided with a semi-thawing step for controlling the temperature inside the container (10) while applying AC voltage to the frozen goods (21), bringing the frozen goods (21) to a semi-thawed state, and a holding step for halting application of AC voltage to the frozen goods (21), and holding the frozen goods (21) while maintaining a semi-thawed state.

IPC Classes  ?

• A23L 3/365 - Thawing subsequent to freezing