[Problem] To provide: orally disintegrating tablets in which the beneficial properties of erythritol are utilized; granules suitable for the production of the orally disintegrating tablets; and a method for producing the granules. [Solution] Erythritol granules for orally disintegrating tablets, each containing 180 parts by weight erythritol, 5 to 50 parts by weight inclusive of crospovidone and 2 to 15 parts by weight inclusive of low-substituted hydroxypropylcellulose and/or crystalline cellulose. According to the present invention, it becomes possible to produce orally disintegrating tablets which have both of proper tablet hardness and proper disintegration time and in which the beneficial properties of erythritol are utilized.
[Problem] To provide an agent for inhibiting the number of bacteria of the genus Fusobacterium and/or bacteria of the genus Sutterella, the agent being capable of effectively inhibiting the number of bacteria of the genus Fusobacterium and bacteria of the genus Sutterella and capable of contributing to the prevention and treatment of diseases such as acute hemorrhagic diarrhea. [Solution] An agent for inhibiting the number of bacteria of the genus Fusobacterium and/or bacteria of the genus Sutterella having 1-kestose as an active ingredient. The present invention can obtain an agent for inhibiting the number of bacteria of the genus Fusobacterium and/or bacteria of the genus Sutterella that can effectively inhibit the number of bacteria of the genus Fusobacterium and bacteria of the genus Sutterella, is highly safe, and can be compounded easily with various foods and pharmaceuticals or taken conveniently on a daily basis.
A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates
[Problem] To provide: a substance and a method both for effectively preventing the worsening of insulin resistance or for ameliorating insulin resistance; a method for treating diabetes, impaired glucose tolerance, obesity, hyperlipemia, arteriosclerosis, hypertension, a heart disease or metabolic syndrome; and a method for preventing the disease. [Solution] An agent for preventing the worsening of insulin resistance or for ameliorating insulin resistance, which contains 1-kestose as an active ingredient. According to the present invention, it becomes possible to prevent the worsening of insulin resistance or ameliorate insulin resistance in a human or animal body conveniently and effectively without little concerning about adverse side effects or safety. According to the present invention, it also becomes possible to prevent or treat diabetes, impaired glucose tolerance, obesity, hyperlipemia, arteriosclerosis, hypertension, a heart disease or metabolic syndrome by preventing the worsening of insulin resistance or ameliorating insulin resistance in a human or animal body.
A61K 31/702 - Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates
A61P 3/08 - Drugs for disorders of the metabolism for glucose homeostasis
A61P 3/10 - Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
A61P 9/00 - Drugs for disorders of the cardiovascular system
A61P 9/10 - Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
[Problem] To provide: erythritol granules which have sufficient bindability while retaining the characteristic properties of erythritol; a method for producing the erythritol granules; a method for producing tablets using the erythritol granules; and tablets. [Solution] Erythritol granules each containing hydroxypropyl cellulose or hydroxypropyl methylcellulose, said erythritol granules having such a property that, when a sample prepared by adding 1.6 mg of magnesium stearate to 160 mg of the erythritol granules is filled in a mortar having a diameter of 8 mm and then the sample is compressed at a compression rate of 10 mm/min under a pressure of 0 to 100 MPa, the average yield pressure at 30 to 100 MPa is less than 2941 MPa. According to the present invention, it becomes possible to produce erythritol granules which have sufficient bindability while retaining the characteristic properties of erythritol.
[Problem] To provide a sugar solution, in which crystal precipitation is suppressed in a low-temperature environment and which can maintain an appropriate viscosity, and a liquid sweetener and a bee feed using the sugar solution. [Solution] A sugar solution which contains sucrose, 1-kestose and glucose, wherein the content of nystose is 0-6 mass% relative to the total amount of sugars, and the content of 1-kestose is 3 parts by weight or more relative to 97 parts by weight of sucrose. According to the present invention, a sugar solution, in which crystal precipitation is suppressed in a low-temperature environment and which can maintain an appropriate viscosity, can be obtained. The sugar solution according to the present invention, which maintains excellent qualities and handling properties even in a low-temperature environment, is usable as a liquid sweetener capable of imparting an excellent flavor to foods and beverages, or a bee feed having excellent handling properties, a long shelf life and a good bee's preference.
[Problem] To provide: an agent for increasing intestinal butyric acid which is capable of effectively increasing intestinal butyric acid; a food composition for increasing intestinal butyric acid; a proliferation agent for butyric acid-producing bacteria; a food composition for proliferating butyric acid-producing bacteria; a method for increasing intestinal butyric acid; a method for proliferating butyric acid-producing bacteria; a method for treating or preventing intestinal inflammation, fatty liver, diabetes, colon cancer or obesity using the same; and use of 1-kestose for producing a drug for treating or preventing the aforesaid diseases. [Solution] An agent for increasing intestinal butyric acid and a proliferation agent for butyric acid-producing bacteria, each comprising 1-kestose as an active ingredient. According to the present invention, intestinal butyric acid in humans or animals can be easily and effectively increased with little side effects or safety concerns. According to the present invention, furthermore, intestinal inflammation, fatty liver, diabetes, colon cancer or obesity can be prevented or treated by increasing intestinal butyric acid in humans or animals.
A61K 31/702 - Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates
A61P 1/04 - Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
[Problem] To provide a substance and method that are capable of effectively inducing proliferation in bacteria of the genus Faecalibacterium, thereby contributing to the prevention and treatment of diseases such as enterocolitis. [Solution] A proliferative agent for bacteria of the genus Faecalibacterium that contains 1-kestose as an active ingredient. In accordance with the present invention, proliferation can be effectively induced in bacteria of the genus Faecalibacterium both in vitro and in vivo. In particular, it is possible to simply and effectively increase in vivo proliferation of bacteria of the genus Faecalibacterium in humans and animals, with almost no concerns regarding side effects or safety. It is also possible to prevent or treat enterocolitis by increasing in vivo proliferation of bacteria of the genus Faecalibacterium in humans and animals.
A61K 31/702 - Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics
A61P 1/04 - Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
[Problem] To provide: a salty taste enhancement method capable of effectively enhancing the salty taste of a food or beverage without compromising the taste or texture thereof; a method for manufacturing a reduced salt food or beverage; and a salty taste enhancer. [Solution] A salty taste enhancement method for a food or beverage, said method comprising a step for adding erythritol to a material of the food or beverage to enhance the salty taste thereof. The present invention is capable of effectively enhancing the salty taste of a food or beverage without compromising the taste or texture thereof.The present invention is also capable of producing a reduced salt food or beverage that gives the perception of a salty taste equivalent to that or stronger than the salty taste before reducing the amount of salt.
[Problem] To provide: a cutting fluid which enables a material being cut to give cut surfaces having improved smoothness and which is effective in diminishing chipping, facilitates the recovery of abrasive grains, and has no fear about safety for the human body; a cutting method; and a smoothness improver for cut surfaces. [Solution] A cutting fluid constituted of a sugar liquid which comprises any sugar alcohol(s) selected from among sorbitol, reduced starch syrups, and reduced maltose syrups and has a viscosity of 9.7 mPa·s or higher. According to the present invention, the cut surface can have improved smoothness and, hence, the polishing step which has conventionally been conducted after cutting can be shortened, simplified, or omitted to greatly contribute to an improvement in working efficiency. Furthermore, since chipping can be diminished, the present invention can greatly contribute to an improvement in the precision of the shape or dimensions of works or to an improvement in yield.
C10M 105/14 - Lubricating compositions characterised by the base-material being a non-macromolecular organic compound containing oxygen having hydroxy groups bound to acyclic or cycloaliphatic carbon atoms polyhydroxy
C10M 129/08 - Hydroxy compounds having hydroxy groups bound to acyclic or cycloaliphatic carbon atoms containing at least 2 hydroxy groups
C10N 30/06 - OilinessFilm-strengthAnti-wearResistance to extreme pressure
C10N 40/22 - Metal working with essential removal of material
Provided is a production method that permits industrial mass production of modified xylopolysaccharides from a biomass. A modified xylopolysaccharide that conserves the modifying group from the side chain of the xylan is obtained by using as the raw material a biomass containing xylan in plant cell walls, the xylan having at least one modifying group selected from the group comprising an acetyl group, feruloyl arabinofuranosyl group, and coumaroyl arabinofuranosyl group in a side chain, and conducting continuous hot-water treatment of a slurry including the biomass in a ratio of 10-30 mass% as the solid content under controlled conditions consisting of a temperature of 160°C or higher, a pressure of the saturated water vapor pressure at this temperature or above, and a reaction severity R0 calculated by formula (1) of 3000-7000 using a cylindrical plug flow reactor equipped internally with a flow path control mechanism for generating a plug flow.
An improved β-fructofuranosidase is provided. The improved β-fructofuranosidase may comprise an amino acid sequence having 60% or higher identity to the amino acid sequence of SEQ ID NO: 2, and may contain an amino acid mutation that replaces histidine (H) corresponding to position 395 counted from the N terminus of SEQ ID NO: 2 with arginine (R) or lysine (K), an amino acid mutation that replaces leucine (L) at position 123 counted from the N terminus of SEQ ID NO: 2 with cysteine (C), and/or an amino acid mutation that replaces phenylalanine (F) at position 473 counted from the N terminus of SEQ ID NO: 2 with tyrosine (Y).
[Problem]
To provide a production method capable of simply and efficiently producing a fructose-added carbohydrate using β-fructofuranosidase.
[Solution]
Escherichia coli and including an amino acid sequence of β-fructofuranosidase.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
C12P 21/04 - Cyclic or bridged peptides or polypeptides, e.g. bacitracin
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
[Problem] To provide an improved β-fructofuranosidase which is capable of efficiently producing kestose while inhibiting the production of nystose. [Solution] This improved β-fructofuranosidase comprises either: an amino acid sequence (a) obtained by introducing, into the amino acid sequence represented by SEQ ID NO: 2, an amino acid mutation i) in which the 85th glycine (G) from the N-terminal is substituted for a protein-constituting amino acid other than glycine (G), and/or an amino acid mutation ii) in which the 310th histidine (H) from the N-terminal is substituted for lysine (K), arginine (R), or tyrosine (Y); or an amino acid sequence (b) which exhibits β-fructofuranosidase activity, and which comprises an amino acid sequence obtained by deleting, substituting, inserting, or adding one or more amino acids in (a) other than the amino acid into which the amino acid mutation has been introduced.
[Problem] To provide an improved β-fructofuranosidase which can produce kestose while preventing the production of nystose and can decompose sucrose while preventing the decomposition of an oligosaccharide. [Solution] An improved β-fructofuranosidase which comprises an amino acid sequence (a) or (b): (a) an amino acid sequence which is produced by introducing an amino acid mutation into an amino acid sequence for a wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2, wherein the amino acid mutation can cause the substitution of a histidine residue (H) located at position-321 as counted from the N-terminal of the amino acid sequence for the wild-type β-fructofuranosidase by a protein-constituting amino acid residue other than a histidine residue (H); and (b) an amino acid sequence which is produced by deleting, substituting, inserting or adding one or several amino acid residues, each of which is different from the amino acid residue into which the amino acid mutation has been introduced, in the amino acid sequence (a), and which has a β-fructofuranosidase activity.
[Problem] To provide an improved β-fructofuranosidase which enables the production of kestose with high efficiency while reducing the amount of a by-product such as nystose produced during the production of kestose. [Solution] An improved β-fructofuranosidase comprising an amino acid sequence which is produced by introducing an amino acid mutation into an amino acid sequence for a β-fructofuranosidase having 60% or higher identity to the amino acid sequence for wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2, wherein the amino acid mutation is such a mutation that, when amino acid sequence alignment is performed, a histidine (H) residue corresponding to the position-395 as numbered from the N-terminal of the amino acid sequence for the wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2 can be replaced by an arginine (R) residue or a lysine (K) residue.
[Problem] To provide a manufacturing method by which a sugar having fructose added thereto can be manufactured simply and efficiently using β-fructofuranosidase. [Solution] A method for manufacturing a sugar having fructose added thereto, said method having a step in which a receptor substrate and a sugar including a fructose residue at the terminal are brought into contact with: Escherichia coli in which an anchor protein for expression on a cell surface and β-fructofuranosidase have been expressed as one polypeptide; and the polypeptide obtained from a composition including dead cells of the Escherichia coli in which the expression has occurred or obtained from the Escherichia coli in which the expression has occurred, said polypeptide including the amino acid sequence of β-fructofuranosidase.
Disclosed is a composition containing a specific oligosaccharide and a bivalent metal cation, which exhibits an effect on the amelioration, treatment or prevention of allergy or topical inflammatory reactions in an extremely safe and effective manner compared to synthetic oral steroid medicines in spite of a fact that the composition contains components that are generally familiar as a food or an ion occurring in living bodies and have no toxicity against living bodies when used at physiological concentrations. Specifically disclosed is a cell-cell adhesion enhancer in epithelial cells, which comprises 1-kestose and/or nystose and a bivalent metal cation as active ingredients. The enhancer can prevent the disruption of an epithelial cell-cell tight junction protein that causes the invasion of an allergen into the body, can repair the epithelial cell-cell tight junction protein or promote the formation of the epithelial cell-cell tight junction protein, whereby the epithelial cell-cell adhesion can be enhanced and the effective amelioration, treatment and prevention of allergic symptoms can be achieved.
Provided is a process by which galactomannan as a substrate can be partially hydrolyzed in a higher concentration, in fewer operation steps and in a shorter time in comparison to conventional processes, and by which a partial hydrolyzate having a molecular structure that achieves a freezing-denaturation inhibitory effect or a stabilizing effect can be efficiently produced. The process comprises a step of subjecting a galactomannan to hydrothermal reaction under such pressure and temperature conditions that neither water nor the galactomannan boils to thereby selectively cleave β-1,4 bonds of the linear backbone chain of the galactomannan and thus partially hydrolyze the galactomannan, the linear backbone chain comprising D-mannose units.
C08B 37/00 - Preparation of polysaccharides not provided for in groups Derivatives thereof
B01J 27/00 - Catalysts comprising the elements or compounds of halogens, sulfur, selenium, tellurium, phosphorus or nitrogenCatalysts comprising carbon compounds
Provided is a substance, minute amounts of which are very effective in improving the survival rate of lactobacillus being stored refrigerated or frozen. The lactobacillus survival rate improvement agent contains, as an active ingredient, a specific strongly moisture-retaining polysaccharide, i.e. galactomannan processed to reduce the molecular weight thereof (low-molecular galactomannan). By adding the lactobacillus survival rate improvement agent to a lactobacillus culture or culture medium such that the final concentration is between 0.01% and 1.0% by weight, the survival rate of lactobacillus being stored refrigerated or frozen can be improved. This also allows the provision of foods and beverages to which a lactobacillus survival rate improvement agent, containing low-molecular galactomannan as an active ingredient, has been added.
Disclosed is a method for improving the activity of a reactive oxygen species (ROS)-scavenging enzyme, which has a mechanism of action based on scientific grounds, is highly safe, is inexpensive, and is widely applicable. An organism having an ROS-scavenging enzyme (e.g., superoxide dismutase, catalase, peroxidase) is administered with one or more substances selected from the group consisting of erythritol, mannitol, sorbitol and xylitol at a dosage concentration of 0.01 to 10% to thereby induce the increase in the amount or the enhancement of the activity of the ROS-scavenging enzyme in the organism.
A highly efficient continuous hydrothermal reaction apparatus for biomass treatment that realizes separation of constituents of biomass as useful resources; and a method of controlling the apparatus. The continuous hydrothermal reaction apparatus is a continuous high-pressure hydrothermal reaction apparatus comprising a material supply unit composed of a material pulverization kneader, a slurry pump capable of controlling a material transport speed and a solvent supply pump; a tube reactor of corrosion resistant metal having its temperature controlled by a multistage temperature raising heater and isothermal heater and a water-cooling type cooler; and a material ejection unit consisting of a duplex electromagnetic shutter valve provided at its center with a pressure buffering chamber, wherein in the tube reactor, the biomass is exposed to strictly controlled high-pressure hydrothermal reaction conditions.
patents
