Abstract

Provided are a cutinase mutant and the use thereof in the efficient degradation of PET. The amino acid sequence of the cutinase mutant is as shown in SEQ ID NO.2, where phenylalanine at position 239 of the amino acid sequence of the wild-type cutinase as shown in SEQ ID NO.1 is replaced with alanine. The cutinase mutant constructed in the present invention is expressed in Escherichia coli. Compared with the wild-type enzyme, the activity of the mutant enzyme shows increased stability, and improved catalytic efficiency, such that the yield of the degradation product of PET plastics (polyethylene terephthalate, PET) is increased by 42.6 times. The modified cutinase mutant improves the PET degradation efficiency.

IPC Classes  ?

• C12N 9/18 - Carboxylic ester hydrolases
• C12N 15/55 - Hydrolases (3)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• B09B 3/60 - Biochemical treatment, e.g. by using enzymes
• C12R 1/19 - Escherichia coli
• B09B 101/75 - Plastic waste

Abstract

358339358339358339358339339 site is of other types, the molecular marker combination is sensitive to Enterocytozoon hepatopenaei.

IPC Classes  ?

• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• C12Q 1/6858 - Allele-specific amplification
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

A novel disturbance-free marine sediment sampling tool, comprising an outer cylinder (2), an inner cylinder (1), a turnover lever assembly (3) and a petal assembly (4). When the sampling tool is moving downwards, the petal assembly (4) is clamped by utilizing an upward acting force of water flow and sediments on the turnover lever assembly (3), so as to keep the petal assembly (4) in an open state during the whole sampling process. When the sampling is completed and the sampling tool starts to be lifted upwards, the petal assembly (4) is pried by utilizing a downward acting force of the sediments on the turnover lever assembly (3), so as to close the petal assembly (4). The sampling tool utilizes the natural resistance of water flow and sediments, etc., and by means of the linkage of the turnover lever assembly (3) and the petal assembly (4), the problem of disturbance and resistance generated by the petal assembly (4) on sediment sampling is effectively solved, and the sampling efficiency is improved while leakage of the sediment sample is effectively blocked.

IPC Classes  ?

• G01N 1/08 - Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit

Abstract

A self-cleaning and anti-adhesion apparatus for marine instruments includes an installation frame. A first end of the installation frame is fixed with an installation base, and a second end of the installation frame is movable with a screw rod. The upper end of the installation base is movable with a first rotation base. A wire netting ball used for holding a sensor is installed above the first rotation base. The wire netting ball is fixedly connected with the installation base. The top end of the screw rod is fixedly connected with a first end of the flexible connector, and a second end of the flexible connector is fixedly connected with a floating bladder. The screw rod is screwed with an inner wheel of an internal meshing ratchet mechanism, and an outer wheel of the internal meshing ratchet mechanism is fixed inside the mounting ring.

IPC Classes  ?

• B08B 1/04 - Cleaning by methods involving the use of tools, brushes, or analogous members using rotary operative members
• B08B 1/00 - Cleaning by methods involving the use of tools
• B63B 59/08 - Cleaning devices for hulls of underwater surfaces while afloat

Abstract

A method and apparatus for inverting a crustal structure of a passive continental margin on the basis of subsidence in a stratigraphic record. The method comprises seven steps. In comparison with the prior art, without the need to additionally provide gravity or refraction seismic data and simply by means of reflection information of a multichannel seism to a stratum, a sedimentary basement and the Moho, the crustal average density, speed and thickness can be quickly and efficiently calculated within a large range to establish a crustal attribute and a depth structure. An effective means is provided for obtaining space variation information of a crustal structure of a passive continental margin that lacks data, and an important basis is thus provided for understanding the formation, basin formation and reservoir formation mechanisms of the passive continental margin.

IPC Classes  ?

• G01V 1/30 - Analysis

Abstract

A deep-sea in-situ long-term experimental platform having a sediment sampling function, which relates to the field of deep sea apparatuses. The platform enables, by means of a multi-stage release mechanism, buoyancy to match gravity to complete an entire sampling recovery process of sediment, which only requires small power units to be sequentially triggered to complete intubation and sampling without a powerful power source to provide kinetic energy for vertical movement. The structure of the deep-sea in-situ long-term experimental platform is simplified, and moreover, the weight is reduced, the cost is reduced, and the reliability is further improved.

IPC Classes  ?

• G01N 1/12 - DippersDredgers
• B63B 35/00 - Vessels or similar floating structures specially adapted for specific purposes and not otherwise provided for

Abstract

Disclosed in the present invention is an intelligent solution injection device for a deep-sea sediment microorganism culture experiment. The intelligent solution injection device is mainly composed of an insertion device, a controllable slow-release solution injection system, an interstitial water displacement and monitoring system, and a deep-sea peristaltic pump. The insertion device can insert a solution injection needle tube and an interstitial water collection tube into a designated sediment position by means of gravity or other driving devices; the controllable slow-release solution injection system is used for receiving an instruction to inject a certain amount of culture solution into sediment through the solution injection needle tube; and the interstitial water displacement and monitoring system is used for collecting sediment interstitial water and monitoring environmental parameters to develop a solution injection policy. The present invention can simulate a diffusion process of matter seeping out of a deep-sea cold seep, and can provide a powerful tool for researchers to conduct long-term in-situ experiments of deep-sea sediment microorganisms.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

Disclosed in the present application are a novel double-sided solid culture dish and use thereof, which relate to the fields of microculture, etc. The culture dish comprises an upper cover and a lower cover, a straight cylinder dish wall being arranged between the upper cover and the lower cover, and the upper cover, the lower cover and the straight cylinder dish wall forming a snap-fit structure; and a gauze type permeable membrane base, a grid-shaped fixing structure being arranged on the gauze type permeable membrane base, and "T"-shaped column structures being vertically arranged on joints or grid lines of the grid-shaped fixing structure. Culture dishes are formed between the gauze type permeable membrane base and the upper cover, and between the gauze type permeable membrane base and the lower cover respectively, and a solidified culture medium is made into the stable double-sided solid culture dish since the "T"-shaped column structures on the gauze type permeable membrane base and a circle of boss structure perpendicular to the wall of the straight cylinder dish wall jointly support a solid culture medium to be not separated from the gauze type permeable membrane base. According to the culture dish, double-sided preparation and the use of the solid culture medium and information exchange and interaction of double-sided microorganisms are achieved, such that related researches of non-contact interaction between microorganisms or interaction between the microorganisms and an environment become simple, convenient, rapid and efficient.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/22 - Petri dishes
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

A self-cleaning anti-adhesion apparatus for a marine instrument, comprising a mounting frame (1), wherein one end of the mounting frame (1) is fixedly provided with a mounting seat (2), the other end of the mounting frame (1) is movably provided with a screw rod (3), and the upper end of the mounting seat (2) is movably provided with a first rotating seat (4); a metal mesh ball (5) used for accommodating a sensor is mounted above the first rotating seat (4), and the metal mesh ball (5) is fixedly connected to the mounting seat (2); the top end of the screw rod (3) is fixedly connected to one end of a flexible connecting piece (6), and the other end of the flexible connecting piece (6) is fixedly connected to a floating bag (7); the screw rod (3) is in threaded connection with an inner wheel of an internally engaged ratchet mechanism (8), an outer wheel of the internally engaged ratchet mechanism (8) is movably mounted on the inner side of a mounting ring (9), and the mounting ring (9) is fixedly connected to the mounting frame (1); the outer wheel of the internally engaged ratchet mechanism (8) is fixedly connected to a second rotating seat (10); a rotating ring (11) is fixedly arranged between the first rotating seat (4) and the second rotating seat (10), and the inner wall of the rotating ring (11) is provided with a brush (12); the rotating ring (11) can be driven by the upper buoyancy of natural wave energy to clean the outer wall of the metal mesh ball (5) without other energy sources, which ensures that the sensor mounted in the metal mesh ball (5) is not obstructed.

IPC Classes  ?

• G01D 11/00 - Component parts of measuring arrangements not specially adapted for a specific variable
• G01D 3/08 - Measuring arrangements with provision for the special purposes referred to in the subgroups of this group with provision for safeguarding the apparatus, e.g. against abnormal operation, against breakdown
• B08B 1/04 - Cleaning by methods involving the use of tools, brushes, or analogous members using rotary operative members

Abstract

Disclosed in the present invention is a natural antimicrobial peptide P-AMP108 isolated from oyster plasma, wherein the natural antimicrobial peptide P-AMP108 has a relatively good ability of clearing gram-positive bacteria such as Propionibacterium acnes, has a high biosafety, and can effectively treat acne caused by Propionibacterium acnes.

IPC Classes  ?

• C07K 7/08 - Linear peptides containing only normal peptide links having 12 to 20 amino acids
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• A61K 38/10 - Peptides having 12 to 20 amino acids
• A61P 17/10 - Anti-acne agents
• A61P 31/04 - Antibacterial agents

Abstract

A grounding segment for a segmented grounding electric device, including a plurality of segment single bodies disposed in a matched manner, wherein a curved concave surface is formed in an outer side surface of each segment single body; a chute is formed in the curved concave surface; an interior of the chute is in sliding connection with an elastic member; two ends of the elastic member are respectively connected to sliders; the sliders are in sliding connection with the interior of the chute; each slider is movably connected to a curved fixing member matching a grounding conductor; the curved fixing member is made of a conductive material applicable to grounding conductors of different sizes; and a locking apparatus is connected between the plurality of segment single bodies. A user conveniently transports and mounts the grounding segment to reduce a land excavation area. Universality of the grounding segment is improved.

IPC Classes  ?

• H01R 4/48 - Clamped connectionsSpring connections using a spring, clip or other resilient member
• H01R 4/64 - Connections between or with conductive parts having primarily a non-electric function, e.g. frame, casing, rail

Abstract

An efficient spawning induction method for Holothuria leucospilota. The method comprises the steps of selecting a parent Holothuria leucospilota, raising the parent Holothuria leucospilota, stimulating same in a shaded and dry environment, stimulating same by exposing in flowing water, injecting same with an oxytocic, placing the parent Holothuria leucospilota in a barrel to await delivery, the parent Holothuria leucospilota spawning, etc.

IPC Classes  ?

• A01K 61/30 - Culture of aquatic animals of sponges, sea urchins or sea cucumbers

Abstract

Disclosed are a Litopenaeus vannamei vibrio-resistance related EST-STR marker, and specific primers thereof and a detection method therefor. A constructed transcriptome library is utilized to develop and obtain six Litopenaeus vannamei EST-STR markers with high polymorphism, numbered Lv-Vp001, Lv-Vp011, Lv-Vp012, Lv-Vp023, Lv-Vp029 and Lv-Vp041, respectively, and specific primers thereof are respectively designed. The Litopenaeus vannamei EST-SSR marker and the primers can be used for genetic diversity research on Litopenaeus vannamei and auxiliary breeding of good varieties.

IPC Classes  ?

• C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

The present invention relates to a grounding node used for a nodal grounding electrical device, comprising a plurality of sets of node units arranged fitted to each other; the outer side of each node unit is provided with an arc-shaped concave surface; the arc-shaped concave surface is provided with a slide groove; the inside of the slide groove is slidingly connected to an elastic member; each of the two ends of the elastic member is connected to a slide block; each of the slide blocks is slidably connected to the inside of the slide groove; each of the slide blocks is movably connected to an arc-shaped fixing member used for fitting with a grounding conductor; the arc-shaped fixing member is made of a conductive material that can adapt to different sizes of grounding conductors; a locking apparatus is connected between the node units. By means of the described technical solution, it is convenient for a user to transport and install a grounding node, reducing the area of land excavation; at the same time, the versatility of the grounding node is improved.

IPC Classes  ?

• H01R 4/66 - Connections with the terrestrial mass, e.g. earth plate, earth pin

Abstract

An environment-friendly composite basalt fiber reef base grid suitable for coral reef substrate restoration and a restoration method, comprising a square grid woven by gel-free basalt fibers. Pure aluminum wires are embedded in each bundle of gel-free basalt fiber; sleeves made of basalt fiber cloth are fixedly connected to four edges of the grid, and a sleeve made of basalt fiber cloth is also provided in the middle of the grid; fixed connection holes are formed in four corners of the grid. The environment-friendly composite basalt fiber reef base grid has been applied to ecological restoration engineering of coastal reefs and islands in the tropical zone of the South China Sea. The accumulated restoration area reaches 4000 m 2, and a good restoration demonstration effect is obtained.

IPC Classes  ?

• A01K 61/00 - Culture of aquatic animals

Abstract

33, 10-15% zeolite powder, 19-44.5% bran powder and 0.5-1.0% trehalose. The sinking-type Rhodosporidium sphaerocarpum microbial preparation contains Rhodosporidium sphaerocarpum ZDFY1801 fermentation paste, said Rhodosporidium sphaerocarpum ZDFY1801 having multiple effects such as efficiently degrading ammonia in water for culturing, fining water for culturing, inhibiting pathogenic Vibrio proliferation in culturing ponds, and promoting cultured animal growth, and being able to applied in aquaculture. When used, the Rhodosporidium sphaerocarpum preparation, which is formed into flat pieces, need only be thrown by hand into the required positions in an aquaculture pond, and the preparation can automatically rapidly sink to the bottom of the pond, and gradually release and begin working.

IPC Classes  ?

• C12N 1/16 - YeastsCulture media therefor
• C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used
• A23K 10/18 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
• C12R 1/645 - Fungi

Abstract

A stable measurement installation support for magnetotellurics magnetobars, comprising insertion rods. The insertion rods are inserted into dug holes or into the ground so as to fix the position of a bottom panel. After the position of the bottom panel is fixed, a level is used to measure whether a horizontal panel is horizontal. If not horizontal, a rotation panel is rotated to adjust four corners of the horizontal panel until the horizontal panel is horizontal, thereby ensuring the normal measurement of the magnetobars. After the horizontal panel is adjusted to be horizontal, a vertical magnetobar or a horizontal magnetobar can be selected at will to be installed and inserted into the ground by means of the insertion rod, thereby stably fixing the magnetobar into a corresponding position without causing shaking. Provided inside of the installation support is a horizontal magnetobar installation seat, and a vertical magnetobar installation seat for installing and fixing a vertical magnetobar and for installing and fixing a horizontal magnetobar. The present invention is highly stable, easy to use, can be installed with high precision, and can effectively enhance work efficiency.

IPC Classes  ?

• G01V 3/08 - Electric or magnetic prospecting or detectingMeasuring magnetic field characteristics of the earth, e.g. declination or deviation operating with magnetic or electric fields produced or modified by objects or geological structures or by detecting devices

Abstract

Provided are specific primers, a method and a related kit for rapid stichopus monotuberculatus sex determination. Specifically, on the basis of a stichopus monotuberculatus XY sex determination mechanism, the present invention screens stichopus monotuberculatus male specific molecular markers, and uses the specific molecular markers as a basis for designing primers Sm-F/Sm-R which may be used for stichopus monotuberculatus sex determination, thereby establishing a method for rapid stichopus monotuberculatus sex determination.

IPC Classes  ?

• C12Q 1/6879 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

An air-sea real-time observation buoy system employing Beidou and Iridium satellite communication, comprising an air-sea observation sensor unit (2000), a data acquisition and communication control unit (3000), an anchoring unit (5000), a buoy platform (1000), a power supply unit (4000), and a shore station data processing center (6000). The data acquisition and communication control unit (3000) is connected to the air-sea observation sensor unit (2000), and is used to acquire and receive data measured by the air-sea observation sensor unit (2000), and transmit the acquired data to the shore station data processing center (6000) by means of combined data transmission communication performed by Beidou satellites and Iridium satellites. Beidou satellite communication is used to transmit data having high security requirements and low data transmission rate requirements. Iridium satellites are used to transmit data having low security requirements and high data transmission rate requirements.

IPC Classes  ?

• B63B 22/00 - Buoys
• B63B 22/04 - Anchoring arrangements
• H02J 7/35 - Parallel operation in networks using both storage and other DC sources, e.g. providing buffering with light sensitive cells
• G01D 21/02 - Measuring two or more variables by means not covered by a single other subclass

Abstract

A method for identifying individual female sea cucumbers having ovaries in development for Holothuria scabra, comprising: extracting pseudocoelom fluid of a Holothuria scabra; performing centrifugal separation to separate out vitellin; performing SDS-PAGE electrophoresis and dyeing; on the basis of the electrophoresis result, distinguishing female sea cucumbers having ovaries in development, male sea cucumbers, and Holothuria scabras having undeveloped reproductive glands. Samples of individual female sea cucumbers having ovaries in development exhibit characteristic bands of 200 to 250kD, and samples of both individuals having undeveloped reproductive glands and individual male sea cucumbers exhibit no characteristic bands. Individual female sea cucumbers having ovaries in development can be identified by extracting pseudocoelom fluid samples while not harming Holothuria scabras, providing application values to the selection of parent sea cucumbers during artificial seedling development.

IPC Classes  ?

• A01K 61/30 - Culture of aquatic animals of sponges, sea urchins or sea cucumbers
• A01K 61/90 - Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C07K 14/775 - Apolipopeptides
• C07K 1/14 - ExtractionSeparationPurification

Abstract

Provided is an artificial ecological reef made of a composite concrete material for the ecological restoration of coral reefs. The artificial ecological reef comprises a concrete reef body, wherein iron fibers are at least distributed on a surface of the concrete reef body. According to the artificial ecological reef of the composite concrete material, a surface is provided with the uniformly distributed high-density and high-carbon cast iron fibers, wherein same are increased by 25% to 30% compared with that in a common concrete reef. The concrete reef has rich three-dimensional structures and may provide more spaces for coral larvae to adhere thereto, and this may improve the adhesion rate of the coral larvae. Moreover, since the carbon content of the high-carbon cast iron fibers is high, iron molecules and carbon molecules in high-iron fibers are likely to form the metal of a galvanic cell, such that an oxidation reaction takes place actively, and the resulting biochemical reaction can promote the calcification growth of hermatypic corals.

IPC Classes  ?

• A01K 61/70 - Artificial fishing banks or reefs

Abstract

An environmentally friendly artificial mimic coral reef body widely applied to the construction of reefs at a coral ecological point and to the reconstruction of three-dimensional structures in a reef area in coral reef ecosystem restoration. For engineering repair of coral reef ecosystems of open sea island reefs, lagoons and offshore waters, an artificial mimic reef body highly similar to natural coral reefs in form and function is constructed through simulation design. This mimic reef body is built using a unique environmentally friendly raw material with an irregular form and has many structural features such as large internal gaps, a rough reef body surface, a large surface area and high spatial heterogeneity, and has advantages such as high hardness, being non-polluting and having a high biological attachment rate. This mimic reef body can provide a growth substrate for critical reef-creating organisms for the coral reef ecosystem such as coral, giant clam and reef-creating seaweed and provide a growth, habitat and reproduction space for other reef protection biological groups.

IPC Classes  ?

• A01K 61/77 - Artificial fishing banks or reefs of monolithic form, e.g. blocks

Abstract

An ansamycin all-carbon cycle polyketide-type antibiotic and uses thereof in preparing antimicrobial medications or anti-tumor medications. Verrucosispora sp. SCSIO 07399 preserved on 22 October 2018 at Guangdong Microbial Culture Collection Center (GDMCC) having the address of: Guangdong Institute of Microbiology, 100 Xianlie Middle Road, Building 59, 5th floor, Guangzhou, and having the preservation number of GDMCC No. 60466. The fermentation culture of Verrucosispora sp. SCSIO 07399 can make new compounds kendomycins B to D, as shown in formulas (I) to (III). Said three compounds have markable antimicrobial and anti-tumor activities.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C07D 493/08 - Bridged systems
• C07D 495/18 - Bridged systems
• A61P 31/04 - Antibacterial agents
• A61P 35/00 - Antineoplastic agents
• C12R 1/01 - Bacteria or actinomycetales

Abstract

An LC/MS and MS automatic switching high-throughput continuous sample feeding apparatus, comprising a column switch valve (1) and a three-way valve (2), a sample feed port of the column switch valve (1) being connected to an automatic sample feeder (3) of a liquid chromatograph, one sample outlet of the column switch valve (1) being connected to a heating flow channel inlet of a first column oven (4) of the liquid chromatograph, a heating flow channel outlet of the first column oven (4) being connected to an inlet of a chromatography column (7), an outlet of the chromatography column (7) being connected to a first sample feed port of the three-way valve (2), another sample outlet of the column switch valve (1) being connected to a heating flow channel inlet of a second column oven (5) of the liquid chromatograph, a heating flow channel outlet of the second column oven (5) being connected to a second sample feed port of the three-way valve (2), a sample outlet of the three-way valve (2) being connected to a sample feed port of a mass spectrometry system (6), the column switch valve (1) being electrically connected to an LC/MS instrument control system. The present invention eliminates complicated manual direct sample feeding operations or frequent chromatography column and two-way replacement procedures, implements LC/MS mode and MS mode detection under a same collection command, and significantly increases operator working efficiency and mass spectrometer utilisation.

IPC Classes  ?

• G01N 30/02 - Column chromatography

Abstract

An apparatus and method for measuring the reflectivity of seabed sediments (12). The apparatus comprises a spectral probe (1), a first whiteboard (2), a second whiteboard (3), a range finder (4) and a rotating shaft (5), wherein the reflectivity of the first whiteboard (2) and the second whiteboard (3) is known, and the first whiteboard (2) and the second whiteboard (3) are connected to the rotating shaft (5) with the first whiteboard and the second whiteboard having spacing along the axial direction of the rotating shaft (5) and being staggered from each other along the radial direction of the rotating shaft (5); the spectral probe (1) is used for acquiring spectral data of the first whiteboard (2), the second whiteboard (3) and seabed sediments (12); the range finder (4) is used for acquiring data of spacing between the first whiteboard (2) and the seabed sediments (12); and the rotating shaft (5) is used for driving the first whiteboard (2) and the second whiteboard (3) to rotate, so that the first whiteboard (2) and the second whiteboard (3) are located in front of the spectral probe (1) in sequence. According to the present invention, the reflectivity of the seabed sediments (12) can be measured by means of only one spectral probe (1), so that the health state of seaweed and coral can be monitored, the production and running costs are low, and the problem of measurement deviation caused by different spectral probes (1) is solved to some extent.

IPC Classes  ?

• G01N 21/55 - Specular reflectivity
• G01N 21/15 - Preventing contamination of the components of the optical system or obstruction of the light path

Abstract

1111 strain obtained by means of space-breeding, the algae strain having the features of a fast growth rate, being capable of simultaneously accumulating high contents of phycocyanin, spirulina polysaccharide and β-carotene, having high outdoor adaptability, and so on. The present invention may be applied to the production of high-quality spirulina powder, the production of phycocyanin, the production of spirulina polysaccharide and spirulina oil rich in β-carotene and like areas.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C07K 14/795 - Porphyrin- or corrin-ring-containing peptides
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12R 1/01 - Bacteria or actinomycetales

Abstract

Sagittula stellataBacillusBacillus sp. SCSIO 43505 microbial agent.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/01 - Bacteria or actinomycetales
• C12R 1/07 - Bacillus

Abstract

Disclosed are an SNP marker related to the low-salt tolerance characteristic of Litopenaeus vannamei, an amplification primer therefor, and an application thereof. A primer is designed on the basis of the mRNA sequence (GenBank: KF765670.1) of the Na,K-ATPase α subunit of Litopenaeus vannamei obtained from NCBI, and the genomic DNA of low-salt tolerant and low-salt intolerant Litopenaeus vannamei are used as templates respectively for PCR amplification to obtain PCR products with a length of 533 bp respectively. The sequencing results are compared and analyzed. A total of 11 SNP sites are detected in this sequence. An SNP marker related to low-salt tolerance is identified by correlative analysis of the genotypes of these templates at each SNP site.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/55 - Hydrolases (3)

Abstract

A preparation method for a mesoporous iron disulfide/silica microsphere composite solid-phase iron source, and an application thereof, using a sol-gel process on a natural pyrite surface to form a mesoporous SiO2 microsphere structure and prepare a mesoporous FeS2/SiO2 microsphere composite solid-phase iron source, and combining same with H2O2 to form a new Fenton oxidation system, for use in removing ciprofloxacin from bodies of water. The mesoporous FeS2/SiO2 microsphere compound solid-phase iron source can both effectively control an iron release rate of a mineral, lowering loss of a mineral iron source itself and operating costs caused by high use of H2O2, and also avoid problems of secondary environmental pollution caused by high release of Fe3+. In addition, the present invention can also increase a number of active sites of contact between H2O2 and pollutants, further promoting full performance of a catalytic reaction and a ciprofloxacin degradation reaction in the system, finally realizing continuous and efficient ciprofloxacin removal.

IPC Classes  ?

• C01G 49/12 - Sulfides
• C02F 1/72 - Treatment of water, waste water, or sewage by oxidation
• C02F 101/30 - Organic compounds

Abstract

The present invention provides a Litopenaeus vannamei osmotic-pressure adjustment-related functional gene EST-SSR label and specific primer thereof and method for detection.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

A system for testing thermophysical properties of rock under a high pressure condition, comprising two pressure resistant tanks (1, 2), a high-pressure pump (3), a temperature monitoring module (8), and a confining pressure monitoring module (9). The high-pressure pump (3) is connected to a first cavity (11) by means of a first connection pipe (5) on which a first bleeder valve (51) and a first pressure sensor (52) are mounted; a rock sample (4) is mounted in a second cavity (21); temperature sensors (61, 62, 63) are respectively mounted on the center and the outer surface of the rock sample (4) and in the second cavity (21); the first cavity (11) is connected to the second cavity (21) by means of a second connection pipe (7) on which a second bleeder valve (71) and a second pressure sensor (72) are mounted. Also disclosed is a method for testing thermophysical properties of rock under a high pressure condition. Without the use of an electrical heating heat source, the system and method can implement instantaneous loading of the rock sample (4) by quickly opening a second bleeder valve (71), and thermophysical property parameters of the rock sample (4) under a high pressure condition are obtained by means of an established finite element numerical inversion model and in combination with a global optimization method.

IPC Classes  ?

• G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity

Abstract

A system and method for testing an adiabatic stress derivative of temperature of underwater rock. The system comprises a first pressure resistant tank (1), a second pressure resistant tank (2), and a third pressure resistant tank (3) which are placed in seawater. A data acquisition unit (11) is mounted in the first pressure resistant tank (1); a rock sample (4) is mounted in a first cavity of the second pressure resistant tank (2); temperature sensors (61, 62, 63) are respectively provided on the outer surface and the center of the rock sample (4) and in the first cavity; a pressure sensor (7) is further provided in the first cavity; output ends of the temperature sensors (61, 62, 63) and an output end of the pressure sensor (7) are all connected to input ends of the data acquisition unit (11); a first bleeder valve (21) in communication with the first cavity is mounted on the second pressure resistant tank (2); a second bleeder valve (22) in communication with the first cavity and a second cavity (31) is further mounted between the second pressure resistant tank (2) and the third pressure resistant tank (3). By monitoring in real time and recording changes of the confining pressure in the second pressure resistant tank (2) and the core temperature of the rock sample (4), the adiabatic stress derivative of temperature of underwater rock can be obtained.

IPC Classes  ?

• G01N 3/08 - Investigating strength properties of solid materials by application of mechanical stress by applying steady tensile or compressive forces
• G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
• G01D 21/02 - Measuring two or more variables by means not covered by a single other subclass

Abstract

A system for testing thermophysical properties of rock under a high pressure condition in deep sea, comprising two pressure resistant tanks (1, 2). A data acquisition unit (3) is mounted in the first pressure resistant tank (1); a cavity of the second pressure resistant tank (2) is filled with seawater and a rock sample (4) is mounted in the cavity; a first temperature sensor (61) and a second temperature sensor (62) are respectively mounted on the center and the outer surface of the rock sample (4); a third temperature sensor (63) and a pressure sensor (7) are mounted in the cavity; output ends of the first temperature sensor (61), the second temperature sensor (62), the third temperature sensor (63), and the pressure sensor (7) are all connected to input ends of the data acquisition unit (3) by means of watertight cables; a bleeder valve (21) in communication with the cavity is mounted on the second pressure resistant tank (2). Also disclosed is a method for testing thermophysical properties of rock under a high pressure condition in deep sea. By means of the method, without the use of an electrical heating heat source and a booster pump, instantaneous loading of a rock sample can be achieved by quickly opening a bleeder valve, and thermophysical properties of the rock sample under a high pressure condition are obtained by means of an established finite element numerical inversion model.

IPC Classes  ?

• G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity

Abstract

A system for testing the temperature response coefficient of rock adiabatic stress changes, comprising two pressure-resistant tanks (A, B) fully filled with silicone oil and rock sample assemblies placed in the pressure-resistant tanks (A, B). The bottoms of the two pressure-resistant tanks (A, B) are connected via an oil pipe, each pressure-resistant tank (A, B) is externally connected with a pressurizing pump via an oil inlet pipe (4), a pressure relief pipe is provided at the top of each pressure-resistant tank (A, B), each of the oil pipe, the oil inlet pipes (4) and the pressure relief pipes (5) is provided with a discharge valve, each of the oil inlet pipes (4) is provided with a pressure sensor (7), each of the rock samples is encapsulated in a rubber sleeve (6) soaked in silicone oil, and the surface and the centre of each rock sample are each provided with a temperature sensor (8). The system is simple and reasonable in structure, and can truly realize the adiabatic pressurization or depressurization of the rock samples, so that changes in the confining pressure of the pressure-resistant tanks (A, B) and the temperature of the rock samples are monitored in real time, so as to accurately acquire the temperature response coefficient of rock adiabatic stress changes.

IPC Classes  ?

• G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity

Abstract

A rock stratum stress variation-temperature response monitoring device, comprising a stress-strain sensor (10) arranged at the bottom of a rock stratum drill hole (40) and filled with expanding cement (50), for detecting the stress variation of the rock stratum; a temperature response amplification component arranged above the stress-strain sensor (10) in the drill hole (40) and also filled with expanding cement (50), for detecting the temperature variation amount induced by the stress variation and amplifying the temperature variation amount; and a power supply control and data collection module (30) arranged outside the drill hole (40), for supplying power to the stress-strain sensor (10) and the temperature response amplification component and collecting the stress variation and the amplified temperature variation amount. Silica gel or rubber with a higher stress-temperature response coefficient is adopted to encapsulate temperature sensors (22), so that the monitored rock stratum drill hole stress-temperature response effect is effectively amplified, and the resolution and sensitivity of the stress and temperature variation monitoring can be greatly improved; moreover, the encapsulation process for the temperature sensors (22) is very simple, the manufacturing is easy and the cost is low.

IPC Classes  ?

• G01L 1/22 - Measuring force or stress, in general by measuring variations in ohmic resistance of solid materials or of electrically-conductive fluidsMeasuring force or stress, in general by making use of electrokinetic cells, i.e. liquid-containing cells wherein an electrical potential is produced or varied upon the application of stress using resistance strain gauges
• G01K 1/08 - Protective devices, e.g. casings

Abstract

The present invention discloses use of an enzyme pair consisting of an aminotransferase and an isomerase in the catalytic formation of L-allo-Ile, the aminotransferase is DsaD represented by SEQ ID NO.7 or MfnO represented by SEQ ID NO.5, the isomerase is DsaE represented by SEQ ID NO.8 or MfnH represented by SEQ ID NO.6. The present invention explains biosynthetic mechanism of L-allo-Ile on enzymatic level, and is of important significance for the preparation of L-allo-Ile by using an enzymatic method and for the diagnosis and treatment of maple syrup urine disease.

IPC Classes  ?

• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine
• C12N 1/20 - BacteriaCulture media therefor
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12R 1/465 - Streptomyces

Abstract

Provided are a vibrio universal suicide vector for gene knockout, a construction method thereof and a use thereof in the vibrio gene knockout. The suicide vector pLP12 is a circular vector comprising a P BAD promoter, a repressor protein gene araC, an RP4 transfer initiation site, a chloramphenicol resistance gene, an R6K replication initiation site, a multiple cloning site region, and a lethal gene vmi480, wherein the multiple cloning site region comprises at least two AhdI enzyme cleavage sites. The suicide vector pLP12 is digested with AhdI to form a linearized suicide vector pLP12T.

IPC Classes  ?

• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/03 - Bacteria
• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/63 - Vibrio

Abstract

A breeding method for obtaining heterosis for a lined seahorse (Hippocampus erectus) comprises the following steps: S1 selecting parents from lined seahorse groups having greatly different genetic backgrounds; S2 intensively nurturing the parents before birth; S3 pairing parents from geographically different lined seahorse groups for breeding according to a complete diallel cross; S4 attentively caring for pregnant seahorses; S5 respectively collecting all hatched larval fishes (filial generations) of each breeding pair within one week; S6 nurturing the filial generations; and S7 comparing survival rates and growth rates of the filial generations. By hybridizing lined seahorses from geographically different groups to obtain hybrids, and effectively using the heterosis, compared with non-hybridized second generations, the survival rates and growth rates of the filial generations are increased significantly, thereby increasing genetic diversity of lined seahorses and facilitating selection of advantageous breeds thereof.

IPC Classes  ?

• A01K 61/00 - Culture of aquatic animals

Abstract

A device capable of realizing data communication with a seafloor heat flow measurement unit comprises a placement support, and a portable signal transmission device; the placement support comprises a supporting unit (1) and a placement unit; the placement unit comprises at least one set of measurement unit sockets, each set of measurement unit sockets comprising a pair of parallel long strip-shaped electrode holders (2), one electrode holder (2) being provided with at least two positive terminals (21), and the other electrode holder (2) being provided with negative terminals (22) in one-to-one correspondence with the number and the positions of the positive terminals (21); and the portable signal transmission device is used for carrying out parameter setting before operation and data reading and storage after operation on the seafloor heat flow measurement unit, and two lines led out from the portable signal transmission device are connected to two electrodes of the measurement unit sockets according to a conventional method. Also provided is a communication method for the seafloor heat flow measurement unit based on the device. Compared with the original method, a large amount of time is saved, and the whole detection experiment efficiency is remarkably increased.

IPC Classes  ?

• G08C 19/00 - Electric signal transmission systems

Abstract

The invention discloses a new strepsesquitriol A, a preparation method thereof and an application thereof. Strepsesquitriol A, a structure as shown in Formula (I), is a compound having a new skeleton and strongly inhibiting the formation of LPS-induced TNFα but showing no cytotoxic activity, so it can be used for the preparation of anti-inflammatory drug or act as a precursor of anti-inflammatory drugs, for the treatment of multiple inflammations. Therefore, the invention provides a new candidate compound for the development of anti-inflammatory drugs, and is of great significance for developing Chinese marine drug resources.

IPC Classes  ?

• C07C 35/37 - Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a ring other than a six-membered aromatic ring polycyclic, at least one hydroxy group bound to a condensed ring system with a hydroxy group on a condensed ring system having three rings
• C12P 7/02 - Preparation of oxygen-containing organic compounds containing a hydroxy group
• C12R 1/465 - Streptomyces
• C12P 5/00 - Preparation of hydrocarbons
• C12P 15/00 - Preparation of compounds containing at least three condensed carbocyclic rings

Abstract

A subsea heat flow long-term observation probe based on an underwater robot platform (300) comprises: a supporting probe rod (10) a plurality of self capacitive temperature measuring units (20). The plurality of self capacitive temperature measuring units (20) is distributed and fixed on the supporting probe rod (10) at an equal interval and in a spiral form, to form a distributed multi-point temperature measuring structure, thereby implementing long-term observation on temperature fluctuation of settlings (200) at different depths under the sea. Each self capacitive temperature measuring unit (20) comprises a housing (25), a battery (24), a temperature measuring circuit board (23), a sensor packaging probe (22), and a temperature sensor (21). The battery (24) and the temperature sensing circuit board (23) are both mounted in the housing (25), the sensor packaging probe (22) is fixed to one end of the housing (25), and the temperature sensor (21) is mounted in the sensor packaging probe (22) and is electrically connected to the temperature measuring circuit board (23). The temperature sensor (21) is in tight contact with the settlings (200) under the sea. Meanwhile, the spiral manner in which the self capacitive temperature measuring units (20) are mounted ensures that each temperature sensor (21) may be in contact with undisturbed settlings (200), and ensures to the greatest extent that the temperature of the settlings (200) is measured rapidly and accurately.

IPC Classes  ?

• G01V 9/00 - Prospecting or detecting by methods not provided for in groups
• G01K 13/02 - Thermometers specially adapted for specific purposes for measuring temperature of moving fluids or granular materials capable of flow

Abstract

An in-situ and on-line acoustic measuring system for natural gas flux at the hydrocarbon seeps at a seafloor includes a seepage tent and a flow measuring channel. The flow measuring channel includes a lower bubble breaking channel, an ultrasonic transducer measuring channel and an upper bubble breaking channel. The lower bubble breaking channel communicates with the seepage tent provided with bubble breaking grids. Lower and upper bubble breaking devices, arranged in a bubble rising direction, are respectively mounted in the lower and upper bubble breaking channels. One side of the ultrasonic transducer measuring channel is fixedly connected with an acoustic wave demultiplexer, and the other side is fixedly connected with flat receiving transducers receiving transmitting acoustic waves generated by an acoustic wave branching unit. Acoustic wave probes, also used for receiving the transmitting acoustic waves generated by the acoustic wave demultiplexer, are arranged in the ultrasonic transducer measuring channel.

IPC Classes  ?

• E21B 47/001 - Survey of boreholes or wells for underwater installations
• E21B 47/10 - Locating fluid leaks, intrusions or movements
• G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters
• E21B 47/00 - Survey of boreholes or wells
• E21B 47/01 - Devices for supporting measuring instruments on drill bits, pipes, rods or wirelinesProtecting measuring instruments in boreholes against heat, shock, pressure or the like
• G01V 9/00 - Prospecting or detecting by methods not provided for in groups

Abstract

A calculation method for a seafloor in-situ thermal conductivity and a detection device using the same, which relate to the technical field of seafloor in-situ heat flow detection. A gesture detection module detects a gesture and the movement condition of a probe at a seafloor, and then a main control module controls a heat pulse control module to excite a heat pulse, so as to perform electric heating on a heating wire of the probe at the front end of a measurement unit; the main control module controls a temperature collection module to collect temperature data of a submarine sediment, and then the temperature data is output to a PC machine; and a finite element numerical model is established by means of the PC machine, and parameter inversion is performed by using a grid encryption search method. The gesture detection module determines seafloor arrival conditions and performs automatic heat pulse control; an in-situ environment temperature and the in-situ thermal conductivity of a submarine sediment are acquired, so that the offshore operation safety and seafloor detection efficiency can be greatly improved; and by establishing an in-situ thermal conductivity numerical inversion method, the limitation of an analytical solution of a simplified model depended by the in-situ thermal conductivity solution is eliminated.

IPC Classes  ?

• G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
• G06F 19/00 - Digital computing or data processing equipment or methods, specially adapted for specific applications (specially adapted for specific functions G06F 17/00;data processing systems or methods specially adapted for administrative, commercial, financial, managerial, supervisory or forecasting purposes G06Q;healthcare informatics G16H)

Abstract

A breeding method for a rapid growth strain of Hippocampus kelloggi. The breeding method comprises: (1) selecting a high-quality Hippocampus kelloggi parent as a breeding base population; (2) intensively culturing before reproduction; (3), performing paired reproduction and culturing bred fry; (4), setting different selection fractions at three critical periods and gradually breeding; (5), pairing selected adult fishes; during pairing, eliminating individuals with poor activity, body injury or pouch or gonadal dysgenesis; afterwards, controlling the Hippocampus kelloggi of each generation to breed at the selection fraction of 10%; and (6), repeating step (2) to step (5) for at least four times to obtain the high-quality Hippocampus kelloggi strain with greatly-improved body weight and body length and stable growth. By means of the breeding method, high-quality and high-yield new strains can be bred, and the problems that the fry of the current Hippocampus kelloggiare are slow in growth, are irregular in size and the like are solved from the germplasm.

IPC Classes  ?

• A01K 61/00 - Culture of aquatic animals

Abstract

A self-floating seafloor heat flow long-term observation station comprises a recycling unit (1), a discarding unit (2) and a cable cutting mechanism (3). The recycling unit (1) is provided with a recycling support, the recycling support is internally provided with two acoustic release transponders (13), the bottoms of the acoustic release transponders (13) are provided with hooks capable of being closed (131), and the recycling support is provided with floating balls (14) in a carrying mode. The discarding unit (2) is provided with a discarding support, and a heat flow probe (24) is fixedly connected below the discarding support. The recycling unit (1) and the discarding unit (2) are fixed together through steel wire ropes (4) of which the two ends are connected with the hooks capable of being closed and arranged at the bottoms of the acoustic release transponders (13). The cable cutting mechanism (3) is fixed to the bottom of the recycling support of the recycling unit (1) and is connected with the steel wire ropes (4) through the movable hooks. Cables (0) start off from the discarding unit (2), then enter the cable cutting mechanism (3), and then penetrate out of the cable cutting mechanism (3) and are connected with the floating balls (14) of the recycling unit (1). The cable cutting mechanism (3) is started to cut off the cables through change of the steel wire ropes (4) from tension to loosening. By means of the base station, the cables can be automatically cut off and/or unplugged, the separation of the recycling unit from the discarding unit is easily achieved, and the base station is suitable for seafloor long-term heat flow observation.

IPC Classes  ?

• G01V 9/00 - Prospecting or detecting by methods not provided for in groups

Abstract

An impact pin-type cable severing mechanism consisting of a blade casing, a cable press plate, an ejection control casing, and a movable hook. Provided within the blade casing are a blade and a powerful compression spring below the blade. The ejection control casing is connected with the movable hook. A trigger piece and a blade latch are provided within the ejection control casing. In the impact pin-type cable severing mechanism, when the hook is taut, the blade latch within the ejection control casing is utilized to lock the blade, thus allowing the compression spring to remain in a compressed state, and when the hook is slack, the trigger piece is impacted by a rebound force to drive the blade latch into separating from the blade, and the compression spring is released to eject the blade to the cable press plate to sever a cable. Also disclosed is a use method for employing the cable severing mechanism in a seafloor observatory. The impact pin-type cable severing mechanism of the present invention is capable of effectively severing a cable in equipment when a relevant instruction is received, thus implementing equipment separation.

IPC Classes  ?

• B21F 11/00 - Cutting wire

Abstract

A dual engaging position-type cable severing mechanism consisting primarily of a blade casing (1), a cable press plate (2), and a movable hook (3). Provided within the blade casing are a blade (12), a compression spring (13) below the blade, and an ejection control unit for controlling the compression spring. In the cable severing mechanism, when the movable hook is taut, the compression spring is controlled by the ejection control unit in the blade casing to remain in a compressed state, and when the movable hook is slack, the compression spring is released by the ejection control unit in the blade casing to eject the blade to the cable press plate to sever a cable (4). A method for using the cable severing mechanism in a seafloor observatory. The cable severing mechanism is capable of effectively severing a cable in equipment when a relevant instruction is received, thus implementing equipment separation. The cable severing mechanism allows simple and effective severance of a cable.

IPC Classes  ?

• B21F 11/00 - Cutting wire

Abstract

Provided herein is a bee venom composition with effects of protecting and beautifying lips, comprising cosmetic matrix components for use on lips and bee venom. The bee venom is prepared by using the following method: crude bee venom is dissolved with water, before ultrafiltrated using an ultrafiltration membrane with the molecular weight cutoff 10000 Da, and then the resulting filtrate is freeze-dried to obtain the bee venom.

IPC Classes  ?

• A61Q 19/00 - Preparations for care of the skin
• A61Q 1/00 - Make-up preparationsBody powdersPreparations for removing make-up
• A61Q 1/04 - Preparations containing skin colorants, e.g. pigments for lips
• A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin

Abstract

An in-situ ultrasonic measurement system for seepage flow of seafloor cold spring natural gas. The system comprises a seepage tent cover (2) and a flow measurement channel (1). The flow measurement channel (1) comprises a lower bubble breaking channel (11), an ultrasonic sensor measurement channel (12) and an upper bubble breaking channel (13) that are arranged from bottom to top in sequence and are communicated with each other. The lower bubble breaking channel (11) is communicated with the seepage tent cover (2) provided with bubble breaking grids (3). A lower bubble breaking device (4) and an upper bubble breaking device (5) that are arranged in the bubble rising direction are respectively mounted in the lower bubble breaking channel (11) and the upper bubble breaking channel (13). One side of the ultrasonic sensor measurement channel (12) is fixedly connected to an acoustic wave branching unit (7), and the other side is fixedly connected to a flat receiving transducer (8) used for receiving transmitting acoustic waves generated by the acoustic wave branching unit (7). An acoustic wave probe (9) also used for receiving the transmitting acoustic waves generated by the acoustic wave branching unit (7) is arranged in the ultrasonic sensor measurement channel (12). The ultrasonic measurement system can be used for performing long-term on-line measurement on in-situ seepage flow of seafloor cold spring natural gas in the environments of different water depths.

IPC Classes  ?

• G01F 1/66 - Measuring the volume flow or mass flow of fluid or fluent solid material wherein the fluid passes through a meter in a continuous flow by measuring frequency, phase shift or propagation time of electromagnetic or other waves, e.g. using ultrasonic flowmeters

Abstract

Provided is a Litopenaeus vannamei sodium/calcium exchanger LvNCX gene and coded protein thereof. The nucleotide sequence of said Litopenaeus vannamei sodium/calcium exchanger LvNCX gene is as shown in SEQ ID NO.1, and the amino acid sequence of the sodium/calcium exchanger LvNCX protein coded thereby is as shown in SEQ ID NO. 2.

IPC Classes  ?

• C12N 15/12 - Genes encoding animal proteins
• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans

Abstract

A streptomycete sesquiterpene triol A, a preparation method therefor and uses thereof. The streptomycete sesquiterpene triol A is separated from a ferment culture of Streptomyces sp.SCSIO10355. The streptomycete sesquiterpene triol A has a high effect on inhibiting LPS-induced TNFα and has no cytotoxic activity, and therefor, the streptomycete sesquiterpene triol A can be used for preparing an anti-inflammatory drug.

IPC Classes  ?

• C07C 35/44 - Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a ring other than a six-membered aromatic ring polycyclic, at least one hydroxy group bound to a condensed ring system with a hydroxy group on a condensed ring system having more than three rings
• C12P 7/02 - Preparation of oxygen-containing organic compounds containing a hydroxy group
• A61K 31/047 - Hydroxy compounds, e.g. alcoholsSalts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
• A61P 29/00 - Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agentsNon-steroidal antiinflammatory drugs [NSAID]

Abstract

Disclosed are a biosynthesis gene cluster of marinacarbolines (MCBs) and a use thereof. The nucleotide sequence of the gene cluster is as shown by positions 1-4222 of SEQ ID NO. 1, and comprises three genes: a gene mcbA responsible for the synthesis of the amide linkage of the MCBs, a gene mcbB responsible for the Pictet-Spengler reaction of MCBs, and a gene mcbC responsible for the decarboxylation of the MCBs.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin

Abstract

A bee venom composition with effects of protecting and beautifying a lip comprises a cosmetic matrix component for use on a lip and comprises bee venom. The bee venom is prepared by using the following method: taking out raw bee venom, dissolving the raw bee venom by using water, performing ultra filtration by using an ultra filtration membrane with the molecular weight cutoff 10000Da, and freezing and drying the filter liquor, so as to obtain the bee venom.

IPC Classes  ?

• A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
• A61K 8/97 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from algae, fungi, lichens or plantsCosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from derivatives thereof
• A61K 8/67 - Vitamins
• A61K 8/92 - Oils, fats or waxesDerivatives thereof, e.g. hydrogenation products
• A61Q 1/04 - Preparations containing skin colorants, e.g. pigments for lips

Abstract

Disclosed is a bee venom composition with a sun-proof effect. The bee venom composition comprises a sun-proof cosmetic substrate component, and further comprises bee venom. According to the present invention, the bee venom is applied in sun-proof cosmetics for a first time, can effectively prevent ultraviolet ray from hurting skin, relieve skin inflammation caused by sunburn, and enable the skin to keep humidification, provide nutrient for the skin, prompt the rehabilitation of hurt skin, and improve the self-protecting capability of the skin. In addition, according to the invention, the bee venom, algal polysaccharides and Vc phosphate magnesium are compounded, thereby remarkably improving effects of the bee venom compound such as sun resistance, inflammation diminishment and repair after being exposed to the sun. The bee venom composition with the sun-proof effect comprises cream, emulsion, water aqua, gelatum, and gel, and is suitable for user groups with various types of skin.

IPC Classes  ?

• A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
• A61K 8/73 - Polysaccharides
• A61Q 17/00 - Barrier preparationsPreparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings

Abstract

A bee venom composition with an acne removing effect comprises a cosmetic matrix component and bee venom. The bee venom is prepared by using the following method: taking out raw bee venom, dissolving the raw bee venom by using water, performing ultra filtration by using an ultra filtration membrane with the molecular weight cutoff 10000Da, and freezing and drying the filter liquor, so as to obtain the bee venom.

IPC Classes  ?

• A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
• A61K 8/73 - Polysaccharides
• A61K 8/36 - Carboxylic acidsSalts or anhydrides thereof
• A61Q 19/00 - Preparations for care of the skin

Abstract

A bee venom composition with a wrinkle removing effect comprises a wrinkle removing cosmetic matrix component and comprises bee venom.

IPC Classes  ?

• A61K 8/73 - Polysaccharides
• A61K 8/98 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution of animal origin
• A61K 8/65 - CollagenGelatinKeratinDerivatives or degradation products thereof
• A61Q 19/08 - Anti-ageing preparations

Abstract

Provided in the present invention are antibiotic Tetrathiazomycin A and the preparation method thereof and the use in the preparation of an antitumour drug. The structural formula of Tetrathiazomycin A is shown as formula (I). The present invention isolated a novel compound Tetrathiazomycin A having an antitumour activity from a fermentation culture of Marinactinospora thermotolerans SCSIO 00652 of the genus Actinomyces, providing a new way of preparing compound Tetrathiazomycin A, and the compound Tetrathiazomycin A of the present invention has a better antitumour activity, so it can be used in an antitumour drug. The present invention also provides a leading compound for the development of antitumour drugs.

IPC Classes  ?

• C07D 277/30 - Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
• C12P 17/16 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing two or more hetero rings
• A61K 31/426 - 1,3-Thiazoles
• A61P 35/00 - Antineoplastic agents
• C12R 1/04 - Actinomyces

Abstract

Disclosed are a marine bacteria cold-adapted protease and an encoded gene and an application thereof. An amino acid sequence of the cold-adapted protease in the present invention is shown in SEQ ID NO.2. A gene of the cold-adapted protease is encoded, and a nucleotide sequence thereof is shown in SEQ ID NO.1. The present invention provides a new cold-adapted protease gene hspa1 for encoding a gene of a cold-adapted protease HSPA2 and a cold-adapted protease HSPA2 encoded by the same. The cold-adapted protease HSPA2 has excellent cold adapted performance and has wide use in protein hydrolysis.

IPC Classes  ?

• C12N 9/52 - Proteinases derived from bacteria
• C12N 15/57 - Hydrolases (3) acting on peptide bonds (3.4)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
• C12R 1/07 - Bacillus

Abstract

Provided is a streptomyces (Streptomyces sp.) strain SCSIO 03032, preserved in China Center for Type Culture Collection on July 18, 2011 with the preservation number CCTCC NO: M 2011258. The streptomyces (Streptomyces sp.) strain SCSIO 03032 can be used to produce compounds Spiro-Indimycin A, B, C and D with antineoplastic activity; the compounds not only have a rare spiral structure, but also have selectivity and highly-efficient activity for tumour cells. Further provided are a preparation method and an application of the compounds Spiro-Indimycin A, B, C and D.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C07D 487/20 - Spiro-condensed systems
• A61K 31/407 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with heterocyclic ring systems, e.g. ketorolac, physostigmine
• A61P 35/02 - Antineoplastic agents specific for leukemia
• A61P 35/00 - Antineoplastic agents
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C12R 1/465 - Streptomyces

Abstract

Provided are a marine streptomyces sp., a compound of pyranosesquiterpenes and a preparation method and the use thereof. Streptomyces sp. SCSIO 01689 was deposited in the China Center for Type Culture Collection (CCTCC) on July 18, 2011, with the deposit number CCTCC NO: M 2011257. Streptomyces sp. can produce a new compound of pyranosesquiterpenes with an anti-Escherichia coli activity and an anti-Artemia sp. activity, and can produce Cyclo(D)-Pro-(D)-Ile, Cyclo(D)-Pro-(D)-Leu and Cyclo(D)-trans-4-OH-Pro-(D)-Phe with an anti-Vibrio anguillarum activity and an anti-Artemia sp. activity; therefore, Streptomyces sp. provides a new approach for preparing Cyclo(D)-Pro-(D)-Ile, Cyclo(D)-Pro-(D)-Leu and Cyclo(D)-trans-4-OH-Pro-(D)-Phe. The compound of pyranosesquiterpenes can be used for preparing anti-Escherichia coli drugs and anti-Artemia sp. drugs, and can also be used as the precursory compound of a flavor agent for the preparation of the flavor agent.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C07D 309/20 - Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hydrogen atoms and substituted hydrocarbon radicals directly attached to ring carbon atoms
• C12P 17/06 - Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
• A61K 31/351 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom not condensed with another ring
• A61P 31/04 - Antibacterial agents
• A01N 43/16 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atom with one hetero atom six-membered rings with oxygen as the ring hetero atom
• A01P 7/00 - Arthropodicides
• C12R 1/465 - Streptomyces

Abstract

Disclosed in the present invention are three cycloheptapeptide compounds; the compounds are isolated from the fermentation products of a marine fungus Acremonium persicinum SCSIO 115, of which the structure is shown in formula (I), wherein R1=H, R2=H in compound 1, R1=OH, R2=H in compound 2 and R1=OH, R2=Me in compound 3. The compounds have an obviously inhibiting effect on the glioma cell strain (SF268), the breast cancer cell strain (MCF-7) and the human large cell non-cancer cell strain (NCI-H460) so can be used for the preparation of anti-tumour drugs and the treatment of tumours.

IPC Classes  ?

• C07K 7/64 - Cyclic peptides containing only normal peptide links
• A61K 38/12 - Cyclic peptides
• A61P 35/00 - Antineoplastic agents

Abstract

Disclosed are a Pseudonocardia sp. and a method for preparing deoxynyboquinone by the same. Pseudonocardia sp. SCSIO 01299 was deposited on July 18, 2011, at the China Center for Type Culture Collection (CCTCC), address: Wuhan University, Wuhan, China, with the deposit number CCTCC NO: M 2011255. Pseudonocardia sp. SCSIO 01299 is able to produce an antibiotic deoxynyboquinone, so Pseudonocardia sp. SCSIO 01299 can be used to prepare deoxynyboquinone, thereby providing a new way for production of the antibiotic deoxynyboquinone having the anti-tumor activity.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C12R 1/01 - Bacteria or actinomycetales

Abstract

A Bacillus barbaricus strain SCSIO 02429(CCTCC NO: M2010213) derived from sea is provided. Also provided is a method for preparing small squid peptide, which comprises adding Bacillus barbaricus strain SCSIO 02429 to fermentation medium inducing enzyme production to ferment and obtain crude enzyme solution used to hydrolyze, then crushing squid livers into pulp, adding the crude enzyme solution into the pulp to enzymatically digest it and obtain crude protein hydrolysate the glycosyl side chains of which are destroyed, adding bromelain to enzymatically digest, keeping the hydrolystae obtained on standing to separate crude fat from water layer, removing fat layer and drying the remains to obtain small squid peptides. The yield rate of small squid peptides by the method of the present application is 40-46%, and the yield rate of amino acids is 16-22%. The small squid peptides can be used to decrease mortality of young maricultural animals and improve growth rate. The small squid peptides also can be used to replace the traditional protein sources in feed such as fish meal, and can be used as functional protein sources and additives in maricultural formulated feed.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
• C12R 1/07 - Bacillus

Abstract

Provided are a peptide extracted from squid viscera and the preparation method thereof, characterized in that the peptide comprises the amino acids fragment -ILGGSDPKHYTG-, the relative molecular mass of the peptide is in the range of 1400-5800, and the peptide is obtained by the following steps: enzymolyzing the viscera of Todarodes pacificus, filtrating with an ultrafiltration membrane with molecular mass cutoff of 18000 Da, isolating and purifying with gel chromatography, thus obtaining a peptide comprising the amino acids fragment -ILGGSDPKHYTG-, then adding potassium sorbate, stirring uniformly and drying. Also provided is a composition consisting of said peptide 6-8%, soybean meal powder 50-60%, fish powder 3-6%, corn meal powder 27-33% and starch for granulation 2-5%. The composition may be used as protein source of feed for marine aquaculture, especially protein source of feed for marine aquatic fish.

IPC Classes  ?

• C07K 2/00 - Peptides of undefined number of amino acidsDerivatives thereof
• C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
• A23K 1/10 - from meat, fish, or bones; from kitchen waste

Abstract

A functional nutriment with effects of promoting lactation and recovery for postpartum women containing marine proteins, papaya and calcium preparation. The functional nutriment also contains Seaweed powder, jujube powder, olive oil, casein phosphopeptide and active probiotics. It's dosage form is powder, effervescent table or suspension. Preparation methods of said functional nutriment are also provided.

IPC Classes  ?

• A61K 35/56 - Materials from animals other than mammals
• A61K 36/185 - Magnoliopsida (dicotyledons)
• A61K 33/06 - Aluminium, calcium or magnesiumCompounds thereof
• A61P 15/14 - Drugs for genital or sexual disordersContraceptives for lactation disorders, e.g. galactorrhoea