Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.
The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
C07K 16/42 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against immunoglobulins (anti-idiotypic antibodies)
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.
A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.
An improved method for the preparation of tetrameric antibody complexes directly on the surface of target entities in a sample is described. In particular, this method involves linking, in the sample, a first target entity with a second target entity in a sample using antibodies with specificity for the first and second target entities.
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/80 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving blood groups or blood types
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C07K 16/44 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere
C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C07K 16/42 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against immunoglobulins (anti-idiotypic antibodies)
An apparatus and method for magnetic separation are provided. The apparatus and method can be used to separate magnetically linked targets from unlinked targets in a suspension. The apparatus has a two-dimensional magnetic array that includes a plurality of one-dimensional magnetic arrays that each define a Halbach array. The one-dimensional arrays are positioned adjacent to one another, and the magnetic fields defined by the plurality of one-dimensional arrays are oriented in the same direction across the array surface. A housing can support the plurality of magnetic elements of the two-dimensional magnetic array. The housing defines a container receiving surface on which a container is receivable adjacent the surface of the magnetic array. A container with a plurality of magnetically linked targets and unlinked targets may be positioned in proximity to the array surface to separate the magnetically linked targets from unlinked targets in the suspension.
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
An improved method for the preparation of tetrameric antibody complexes directly on the surface of target entities in a sample is described. In particular, this method involves linking, in the sample, a first target entity with a second target entity in a sample using antibodies with specificity for the first and second target entities.
C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
A61K 31/197 - Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
A61K 31/198 - Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
A61K 31/221 - Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin with compounds having an amino group, e.g. acetylcholine, acetylcarnitine
A61K 31/385 - Heterocyclic compounds having sulfur as a ring hetero atom having two or more sulfur atoms in the same ring
This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
10.
Soluble antibody complexes for T cell or NK cell activation and expansion
This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.
This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
The present disclosure provides a method of determining the frequency and proliferative potential of progenitor cells in a starting cell preparation comprising (a) culturing the cell preparation for 5-10 days in a culture medium comprising FLT-3 Ligand (FL) and Thrombopoietin (TPO); (b) detecting and enumerating the colonies; and (c) assessing the frequency and proliferative potential of progenitor cells in the starting cell preparation based on the number of colonies in step (b). The number of colonies in step (b) correlates to the number of colonies in a standard 14-day colony-forming cell assay. The method is particularly useful for predicting and evaluating the suitability of grafts for transplantation.
G01N 33/567 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent utilising isolate of tissue or organ as binding agent
A cell culture device comprises a well. A plurality of microwells are within the well, and a first common fluid volume is within the well above the microwells. A set of sub-microwells are within each microwell, and a second common fluid volume is within each microwell above the set of sub-microwells.
The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B05D 1/00 - Processes for applying liquids or other fluent materials
B05D 1/18 - Processes for applying liquids or other fluent materials performed by dipping
B05D 5/08 - Processes for applying liquids or other fluent materials to surfaces to obtain special surface effects, finishes or structures to obtain an anti-friction or anti-adhesive surface
C09D 183/08 - Polysiloxanes containing silicon bound to organic groups containing atoms other than carbon, hydrogen, and oxygen
C08G 77/24 - Polysiloxanes containing silicon bound to organic groups containing atoms other than carbon, hydrogen, and oxygen halogen-containing groups
16.
METHOD FOR SEPARATING TARGET ENTITIES FROM A SAMPLE USING A COMPOSITION OF MONO-SPECIFIC TETRAMERIC ANTIBODY COMPLEXES COUPLED TO A SURFACE
An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
B01D 37/02 - Precoating the filtering elements or materialAddition of filter aids to the liquid being filtered
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
C12N 5/0787 - Granulocytes, e.g. basophils, eosinophils, neutrophils or mast cells
B01D 37/02 - Precoating the filtering elements or materialAddition of filter aids to the liquid being filtered
C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
18.
Method for separating target entities from a sample using a composition of mono-specific tetrameric antibody complexes coupled to a surface
An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12N 5/078 - Cells from blood or from the immune system
C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
22.
COMPOSITIONS AND METHODS FOR OBTAINING ENRICHED MESENCHYMAL STEM CELL CULTURES
The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
C09K 3/18 - Materials not provided for elsewhere for application to surface to minimize adherence of ice, mist or water theretoThawing or antifreeze materials for application to surfaces
24.
Cell culture vessels for meniscus reduction with aqueous solutions
A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.
This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.
The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
A filter apparatus comprises a filter having first and second opposed surfaces. A first reservoir is positioned adjacent with the first surface and in communication with the first surface, and a first inlet-outlet port is in communication with the first reservoir and spaced from the first surface. A second reservoir is positioned adjacent the second surface, and in communication with the second surface, and a second inlet-outlet port is in communication with the second reservoir and spaced from the second surface. A filter plate system comprises a reservoir plate. The reservoir plate comprises at least one reservoir well. The filter plate system further comprises a strainer plate. The strainer plate comprises at least one strainer well, which is removably receivable in the reservoir well. The strainer well comprises at least one mesh wall portion.
A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.
C12N 1/02 - Separating microorganisms from their culture media
C40B 30/10 - Methods of screening libraries by measuring physical properties, e.g. mass
H01F 1/11 - Magnets or magnetic bodies characterised by the magnetic materials thereforSelection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles
B82Y 5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
B82Y 25/00 - Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
C12N 11/10 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
C08L 1/00 - Compositions of cellulose, modified cellulose, or cellulose derivatives
C08L 3/00 - Compositions of starch, amylose or amylopectin or of their derivatives or degradation products
C08L 5/00 - Compositions of polysaccharides or of their derivatives not provided for in group or
The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture medium having an osmolality less than 340 mOsm/kg. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media for use in inducing the three germ layers.
The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture medium having an osmolality less than 340 mOsm/kg. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media for use in inducing the three germ layers.
A filter apparatus comprises a filter having first and second opposed surfaces. A first reservoir is positioned adjacent with the first surface and in communication with the first surface, and a first inlet-outlet port is in communication with the first reservoir and spaced from the first surface. A second reservoir is positioned adjacent the second surface, and in communication with the second surface, and a second inlet-outlet port is in communication with the second reservoir and spaced from the second surface. A filter plate system comprises a reservoir plate. The reservoir plate comprises at least one reservoir well. The filter plate system further comprises a strainer plate. The strainer plate comprises at least one strainer well, which is removably receivable in the reservoir well. The strainer well comprises at least one mesh wall portion.
A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contacting other pipette tips in the pipette tip holder.
A magnetic particle comprises a polysaccharide maxtrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.
H01F 1/113 - Magnets or magnetic bodies characterised by the magnetic materials thereforSelection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles in a bonding agent
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
41.
METHOD OF REDUCING CURVATURE IN A MENISCUS OF LIQUID MEDIUM
The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
A pipette tip holder is provided for holding a plurality of pipette tips in an automated pipette machine. The pipette tip holder includes a container that defines a cavity. There is at least one vertical divider in the cavity that divides the cavity into a plurality of segregated compartments. The pipette tip holder further includes a tip support member. The tip support member is connected to the container. The tip support member defines a plurality of apertures. The apertures are sized to receive and suspend said pipette tips in the cavity. Each aperture is generally aligned with one of said compartments. The at least one vertical divider is sized and positioned to inhibit contact between bottom portions of adjacent pipette tips.
f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD31 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD31 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
patents
