Abstract

Provided herein systems and methods for processing cryopreserved cells without an expansion phase after thawing, including transfecting cells for gene editing purposes and cryopreserving transfected cells. Systems and methods for high-throughput processing of frozen cells are also provided. The systems and methods provided can be used to modify cells, including gene editing, in cell arrays.

IPC Classes  ?

• A01N 1/126 - Physiologically active agents, e.g. antioxidants or nutrients
• A01N 1/128 - Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gelsChemically altering living parts, e.g. by cross-linking
• C12N 5/074 - Adult stem cells
• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C07F 9/655 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms

Abstract

A method for manufacturing nucleic acid molecules, including: obtaining, using a processor, a plurality of fractions from a nucleic acid synthesis procedure; obtaining, using the processor, characterization information regarding each of the plurality of fractions, the characterization information including mass spectrometry and liquid chromatography data for each of the plurality of fractions; identifying, using the processor, a subset of the plurality of fractions to combine to generate a simulated pool based on a metric; simulating, using the processor, a predicted metric for the simulated pool based on identifying the subset of the plurality of fractions to combine; and providing, using the processor, information identifying the subset of fractions to a user to combine into a combined pool based on simulating the predicted metric for the simulated pool.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Provided herein are methods for assessing a preparation of guide RNA molecules for quality, including for purity and identity.

IPC Classes  ?

• C12Q 1/6869 - Methods for sequencing
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6855 - Ligating adaptors
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Provided herein are methods for assessing a preparation of guide RNA molecules for quality, including for purity and identity.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

A reactor system is disclosed. The reactor system includes a vessel configured to contain a solid support, the vessel including: a vessel wall defining a reaction chamber, the reaction chamber having a first end and a second end opposite the first end; a piston operatively arranged at the first end and configured to translate within the reaction chamber; a force measuring device coupled to the piston and configured to measure a load on the piston; a piston driver coupled to the piston; and a processor operably coupled to the force measuring device and the piston driver, the processor configured to: measure a load on the piston using the force measuring device, and adjust a position of the piston using the piston driver based on measuring the load on the piston.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus

Abstract

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A reactor system is disclosed. The reactor system includes a vessel configured to contain a solid support, the vessel including: a vessel wall defining a reaction chamber, the reaction chamber having a first end and a second end opposite the first end; a piston operatively arranged at the first end and configured to translate within the reaction chamber; a force measuring device coupled to the piston and configured to measure a load on the piston; a piston driver coupled to the piston; and a processor operably coupled to the force measuring device and the piston driver, the processor configured to: measure a load on the piston using the force measuring device, and adjust a position of the piston using the piston driver based on measuring the load on the piston.

IPC Classes  ?

• B01J 19/18 - Stationary reactors having moving elements inside
• B01F 31/65 - Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• B01J 8/16 - Chemical or physical processes in general, conducted in the presence of fluids and solid particlesApparatus for such processes with particles being subjected to vibrations or pulsations
• B01J 14/00 - Chemical processes in general for reacting liquids with liquidsApparatus specially adapted therefor

Abstract

A cell centrifugation consumable and a method of using the cell centrifugation consumable is disclosed. The cell centrifugation consumable includes an inner fluid chamber comprising a tapered portion having a collection chamber at a narrow end of the tapered portion. The inner fluid chamber is configured to contain a cell solution. Upon centrifugation of the cell solution, a cell pellet forms within the collection chamber. The cell centrifugation consumable further includes a supernatant fluid port fluidly couplable to the collection chamber configured to allow transfer of supernatant from the collection chamber. The cell centrifugation consumable further includes a sensor detection line within the collection chamber configured so that a sensor can detect the presence of a supernatant or a cell pellet at the sensor detection line. The cell centrifugation consumable further includes a piston configured to translate the cell pellet within the collection chamber toward the sensor detection line.

IPC Classes  ?

• B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
• B04B 15/12 - Other accessories for centrifuges for drying or washing the separated solid particles
• C12M 1/28 - Inoculator or sampler being part of container
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
• B04B 11/06 - Arrangement of distributors or collectors in centrifuges

Abstract

The application relates to biological components, methods, systems, and kits for modulating immune responses. The disclosed biological components include genetically modified cells comprising an inserted exogenous sequence in a major histocompatibility complex (MHC)-associated gene. The inserted exogenous sequence encodes a peptide and the genetically modified cells express a fusion protein comprising the peptide and at least a portion of the polypeptide encoded by the MHC-associated gene to form a modified MHC complex. The genetically modified cells may present the peptide as an antigen associated with the MHC complex and may be utilized in methods for modulating T cell activity, inducing an immune response, and inducing a tolerogenic response. As such, the disclosed biological components, methods, systems, and kits may be utilized in order to treat and/or prevent a disease or disorder in a subject in need thereof and to screen and validate clinically relevant antigens.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• C07K 14/74 - Major histocompatibility complex [MHC]
• C12N 5/0781 - B cellsProgenitors thereof
• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

An electroporator circuit and electroporator device can be used to deliver an electroporation signal to an electroporation load. The electroporator circuit can include an energy source, a filter, a converter, and a controller. The output of the electroporator circuit can be controlled based on an arbitrary waveform provided as input to the controller.

IPC Classes  ?

• A61N 1/32 - Applying electric currents by contact electrodes alternating or intermittent currents
• H03K 3/02 - Generators characterised by the type of circuit or by the means used for producing pulses
• H03K 7/08 - Duration or width modulation
• A61B 18/12 - Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating by passing a current through the tissue to be heated, e.g. high-frequency current

Abstract

A membrane valve system, including: an etched substrate including a network of channels defining a plurality of valve connection points arranged in an arcuate pattern; a deformable membrane including a plurality of raised valve elements complementary to the plurality of valve connection points; and a rotary valve pin including a valve face including a plurality of valve controlling mechanisms, the plurality of valve controlling mechanisms including a pair of valve controlling mechanisms complementary to a pair of raised valve elements of the plurality of raised valve elements, the valve face being configured to depress each of the plurality of raised valve elements to maintain each in a closed position, and the pair of valve controlling mechanisms of the valve face being configured to permit the pair of raised valve elements of the plurality of raised valve elements to be in an open position.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• F16K 11/02 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

A cell centrifugation consumable and a method of using the cell centrifugation consumable is disclosed. The cell centrifugation consumable includes an inner fluid chamber configured to hold cells during centrifugation and a decanting space. The inner fluid chamber includes a supernatant fluid port that permits drawing off of supernatant from the inner fluid chamber, and a decanting port that permits a portion of the supernatant to flow into a decanting space when the cell centrifugation is tipped approximately 90°. Together, the supernatant fluid port and the decanting port allow the cell centrifugation consumable to remove the supernatant without knowing the volume of the supernatant. The cell centrifugation consumable further includes a collection chamber fluid port configured to flow additional fluid into to the cell centrifugation consumable after centrifugation, wherein the additional fluid resuspends the cell pellet.

IPC Classes  ?

• B04B 11/04 - Periodical feeding or dischargingControl arrangements therefor
• B04B 15/12 - Other accessories for centrifuges for drying or washing the separated solid particles
• C12M 1/28 - Inoculator or sampler being part of container
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
• B04B 11/06 - Arrangement of distributors or collectors in centrifuges

Abstract

Provided herein are polynucleotides and genetically modified cells comprising polynucleotides encoding a genetically modified major histocompatibility complex (MHC) associated polypeptide linked to an adapter peptide that is capable of binding to a targetable binder moiety and modified antigens. Methods of making and using the disclosed polynucleotides, cells and modified antigens are also provided.

IPC Classes  ?

• A61K 35/12 - Materials from mammalsCompositions comprising non-specified tissues or cellsCompositions comprising non-embryonic stem cellsGenetically modified cells
• A61K 35/18 - Erythrocytes
• A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• A61K 35/00 - Medicinal preparations containing materials or reaction products thereof with undetermined constitution
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

IPC Classes  ?

• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides

Abstract

A modular liquid patch panel (LPP) for providing one or more fluid pathways between a first processing module and a second processing module for culturing or processing cells, wherein: the first processing module comprises one or more liquid ports, and one or more air ports; and the second processing module comprises one or more liquid ports, and one or more air ports; and the LPP comprises one or more manifolds comprising one or more fluid pathways, a first connector that engages a liquid port on the first processing, module, and a second connector that engages a liquid port on the second processing module such that a fluid pathway is provided from the first processing module, through the LPP, and to the second processing module, and liquid is passed through the fluid pathway by introducing or removing air from the first processing module and/or the second processing module.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12M 1/32 - Inoculator or sampler multiple field or continuous type

Abstract

Various embodiments of the disclosure provide a cell processing system including: a liquid patch panel (LPP) including a flow coupler and a first flow path at least partially defined by the flow coupler. The flow coupler may include: a lumen; a hollow tube (e.g. a needle or cannula) positioned within the lumen; and a first barrier (e.g. a septum such as an engageable septum) that isolates the lumen from the ambient environment. The cell processing system may further include a container (e.g. a consumable) including a second barrier (e.g. a septum) that seals an internal volume of the container from the ambient environment. The flow coupler may be configured to move the first barrier into contact with the second barrier of the container while the hollow tube is positioned within the lumen and to move the hollow tube towards the first barrier until the hollow tube opens the first barrier, opens the second barrier, and enters into the internal volume of the container. In various embodiments of the cell processing system, the hollow tube may be presterilized.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• B01D 63/08 - Flat membrane modules
• B01D 69/06 - Flat membranes
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells

Abstract

A system for processing cells can be provided. The system can include a control board, a gas source fluidly coupled to the control board, a process board that is engageable with the control board to fluidly couple the process board to the control board, and a processing module that is engageable with the process board to fluidly couple the processing module to the process board. The gas source can be configured to drive gas into or out of the control board to move liquid including cells suspended therein through the processing module. The processing module can be configured to perform a process on the cells as the liquid including the cells pass through the cell processing module.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

An Integrated fully automated closed fluid handling system includes a set of fill-finish aseptic cartridges filled at least partially with a liquid or lyophilized and/ or dried, reagents, a microfluidic or fluidic conduit, a reusable or disposable consumable with channels and hollow tubes or syringes used for piercing a septum on the cartridges, integrating an actuator to push cartridges towards hollow tubes to pierce the septum or seal such that the liquids are transferred from the cartridges to the microfluidic or fluidic conduit, a robot arm or tweezer to transfer the cartridges into a consumable, a barcode scanner to confirm the QR code on the cartridges, a consumable handling system, a flow control system to provide automated control of various aspects of the liquid handling process. The integrated and fully automated closed fluid handling system may include a cartridge retraction mechanism to retract the cartridges.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• A61M 5/14 - Infusion devices, e.g. infusing by gravityBlood infusionAccessories therefor
• A61M 3/02 - EnemataIrrigators
• B01L 9/00 - Supporting devicesHolding devices

Goods & Services

Chemically synthesized preparations in the nature of separate genome editing components, namely, guide RNA (gRNA) for research and clinical testing purposes, other than for medical or veterinary use Documentation, namely, printed reports in the field of chemically synthesized preparations in the nature of separate genome editing components being guide RNA (gRNA) for research and clinical testing purposes

Abstract

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compartment configured for performing cell selection, wherein an inlet of the second compartment is operably coupled to an outlet of the first compartment, and wherein the second compartment further comprises at least one optically-transparent wall and an outlet that is operably coupled to an intermediate cell removal port; and c) a third compartment configured for performing cell expansion, wherein an inlet of the third compartment is operably coupled to the outlet of the second compartment.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

IPC Classes  ?

• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Abstract

The application relates to biological components, methods, systems, and kits for modulating immune responses. The disclosed biological components include genetically modified cells comprising an inserted exogenous sequence in a major histocompatibility complex (MHC)-associated gene. The inserted exogenous sequence encodes a peptide and the genetically modified cells express a fusion protein comprising the peptide and at least a portion of the polypeptide encoded by the MHC-associated gene to form a modified MHC complex. The genetically modified cells may present the peptide as an antigen associated with the MHC complex and may be utilized in methods for modulating T cell activity, inducing an immune response, and inducing a tolerogenic response. As such, the disclosed biological components, methods, systems, and kits may be utilized in order to treat and/or prevent a disease or disorder in a subject in need thereof and to screen and validate clinically relevant antigens.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies

Abstract

A system for processing cells is provided. The system can include a cell culture container, a fluid handling device, and one or more removable cell processing modules for performing one or more cell processing processes. The one or more removable cell processing modules can include a fluid handling pathway. The one or more removable cell processing modules can be fluidly connected to the cell culture container and the fluid handling device via a receptacle in which the cell processing modules may be inserted. The system can be a closed system.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means

Abstract

A system for processing cells is provided. The system can include a cell culture container, a fluid handling device, and one or more removable cell processing modules for performing one or more cell processing processes. The one or more removable cell processing modules can include a fluid handling pathway. The one or more removable cell processing modules can be fluidly connected to the cell culture container and the fluid handling device via a receptacle in which the cell processing modules may be inserted. The system can be a closed system.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 3/02 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means

Abstract

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C07F 9/655 - Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms

Goods & Services

Advertising, marketing and promotional services, namely, organization of a trade fair or an exhibition for commercial or advertising purposes. Educational services, namely, the hosting of a conference, symposium or congress on CRISPR related research and development.

Abstract

Disclosed herein are cartridges for transfecting cells and/or generating clonal populations of cells comprising: a) a first compartment configured for performing cell transfection, wherein the first compartment comprises a first inlet configured for introduction of a cell sample; b) a second compartment configured for performing cell selection, wherein an inlet of the second compartment is operably coupled to an outlet of the first compartment, and wherein the second compartment further comprises at least one optically-transparent wall and an outlet that is operably coupled to an intermediate cell removal port; and c) a third compartment configured for performing cell expansion, wherein an inlet of the third compartment is operably coupled to the outlet of the second compartment.

IPC Classes  ?

• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 5/02 - Propagation of single cells or cells in suspensionMaintenance thereofCulture media therefor
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

IPC Classes  ?

• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Goods & Services

Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; assays for research purposes; biochemical reagents used for non-medical purposes; biochemicals, namely, precursors for in vitro genetic engineering use; cells for scientific and research use; cells for scientific, laboratory or medical research; chemical reagents for non-medical purposes; chemical reagents, other than for medical or veterinary purposes; chemical test kits for molecular biology research for laboratory or research use; diagnostic preparations for scientific or research use other than for medical use; diagnostic reagents for scientific use; diagnostic reagents for scientific or research use; reagents for research purposes; reagents for scientific and research use; reagents for scientific or medical research use; assays and reagents for use in genetic research; biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; diagnostic reagents and preparations, except for medical or veterinary use; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; reagents for use in scientific apparatus for chemical or biological analysis. Reagents, assays and cells for medical use. Biochemical research and development; biotechnology research; dna analysis services for scientific research purposes; dna screening for scientific research purposes; genetic mapping for scientific purposes; genetic testing for scientific research purposes; genetic testing of laboratory animals for research purposes; scientific research; scientific research and development; consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; custom design and development of chemical reagents and biochemical assays; design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto (terms too vague in the opinion of the International Bureau – Rule 13 (2) (b) of the Regulations); research and development in the pharmaceutical and biotechnology fields; scientific research in the field of genetics and genetic engineering.

Abstract

Described herein are methods for generating and using variant panels of clonally expanded cells containing a plurality of introduced genetic variants. These clonally expanded cells can be partitioned such that each individual partition contains a single genetic variant, allowing for the assessment of the outcome of each variant without the confounding effect of background genetic variation. Further, panels of such variants can be used to evaluate nucleic acid repair strategies. Genetic variation can be introduced through the use of genome editing tools, such as CRISPR/Cas.

IPC Classes  ?

• C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving viable microorganisms
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Goods & Services

Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; Assays for research purposes; Biochemical reagents used for non-medical purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Cells for scientific and research use; Cells for scientific, laboratory or medical research; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes; Chemical test kits for molecular biology research for laboratory or research use; Diagnostic preparations for scientific or research use other than for medical use; Diagnostic reagents for scientific use; Diagnostic reagents for scientific or research use; Reagents for research purposes; Reagents for scientific and research use; Reagents for scientific or medical research use; Assays and reagents for use in genetic research; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Reagents for use in scientific apparatus for chemical or biological analysis. Reagents and cells for medical use. Biochemical research and development; Biotechnology research; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; Genetic mapping for scientific purposes; Genetic testing for scientific research purposes; Genetic testing of laboratory animals for research purposes; Scientific research; Scientific research and development; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the field of genetics and genetic engineering.

Abstract

The present invention provides improved automated systems and methods for synthesis of biopolymers including DNA and RNA. The automated systems and methods represent a number of improvements over existing systems for multiplex synthesis of biopolymers in a combinatorial fashion.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• C07H 1/00 - Processes for the preparation of sugar derivatives
• C07K 1/04 - General processes for the preparation of peptides on carriers
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C40B 50/14 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support

Goods & Services

Reagents, assays and cells for medical use; reagents, assays and cells for use in the further manufacture of pharmaceuticals, diagnostics and therapeutics.

Goods & Services

Advertising, marketing and promotional services, namely, organization of a trade fair or an exhibition for commercial or advertising purposes Educational services, namely, the hosting of a conference, symposium or congress on CRISPR related research and development

Abstract

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12P 1/20 -

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Goods & Services

(1) Clinical and diagnostic reagents and cells for medical use; reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics.

Goods & Services

(1) Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use; biochemical reagents used for non-medical purposes, namely for editing cells; biochemicals, namely, precursors for in vitro genetic engineering use; cells for scientific and research use; cells for scientific, laboratory or medical research; chemical reagents for non-medical purposes; chemical reagents, other than for medical or veterinary purposes; chemical test kits for molecular biology research for laboratory or research use; diagnostic preparations for medical-scientific research use; diagnostic reagents for medical-scientific research use; reagents for medical-scientific research use; assays and reagents for use in genetic research; biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; reagents for use in scientific apparatus for chemical or biological analysis. (2) Clinical and diagnostic reagents and cells for medical use. (1) Biochemical research and development; biotechnology research; DNA analysis services for scientific research purposes; genetic mapping for scientific purposes; scientific research in the fields of reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics, biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use, biochemical reagents used for non-medical purposes, namely for editing cells, biochemicals, namely, precursors for in vitro genetic engineering use, cells for scientific and research use, cells for scientific, laboratory or medical research, chemical reagents for non-medical purposes, chemical reagents, other than for medical or veterinary purposes, chemical test kits for molecular biology research for laboratory or research use, diagnostic preparations for medical-scientific research use, diagnostic reagents for medical-scientific research use, reagents for medical-scientific research use, assays and reagents for use in genetic research, biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes, chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid, diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology, nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, reagents for use in scientific apparatus for chemical or biological analysis, clinical and diagnostic reagents and cells for medical use; scientific research and development in the fields of in the fields of reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics, biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use, biochemical reagents used for non-medical purposes, namely for editing cells, biochemicals, namely, precursors for in vitro genetic engineering use, cells for scientific and research use; cells for scientific, laboratory or medical research, chemical reagents for non-medical purposes, chemical reagents, other than for medical or veterinary purposes, chemical test kits for molecular biology research for laboratory or research use, diagnostic preparations for medical-scientific research use, diagnostic reagents for medical-scientific research use, reagents for medical-scientific research use, assays and reagents for use in genetic research, biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes, chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid, diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology, nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, reagents for use in scientific apparatus for chemical or biological analysis, clinical and diagnostic reagents and cells for medical use; consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; custom design and development of chemical reagents and biochemical assays; design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; research and development in the pharmaceutical and biotechnology fields; scientific research in the field of genetics and genetic engineering.

Goods & Services

(1) Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use; biochemical reagents used for non-medical purposes, namely for editing cells; biochemicals, namely, precursors for in vitro genetic engineering use; cells for scientific and research use; cells for scientific, laboratory or medical research; chemical reagents for non-medical purposes; chemical reagents, other than for medical or veterinary purposes; chemical test kits for molecular biology research for laboratory or research use; diagnostic preparations for medical-scientific research use; diagnostic reagents for medical-scientific research use; reagents for medical-scientific research use; assays and reagents for use in genetic research; biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; reagents for use in scientific apparatus for chemical or biological analysis. (2) Clinical and diagnostic reagents and cells for medical use (1) Biochemical research and development; biotechnology research; DNA analysis services for scientific research purposes; genetic mapping for scientific purposes; scientific research in the fields of reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics, biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use, biochemical reagents used for non-medical purposes, namely for editing cells, biochemicals, namely, precursors for in vitro genetic engineering use, cells for scientific and research use, cells for scientific, laboratory or medical research, chemical reagents for non-medical purposes, chemical reagents, other than for medical or veterinary purposes, chemical test kits for molecular biology research for laboratory or research use, diagnostic preparations for medical-scientific research use, diagnostic reagents for medical-scientific research use, reagents for medical-scientific research use, assays and reagents for use in genetic research, biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes, chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid, diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology, nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, reagents for use in scientific apparatus for chemical or biological analysis, clinical and diagnostic reagents and cells for medical use; scientific research and development in the fields of in the fields of reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics, biological assays for use in the fields of biological and diagnostic testing for industrial, scientific and research use, biochemical reagents used for non-medical purposes, namely for editing cells, biochemicals, namely, precursors for in vitro genetic engineering use, cells for scientific and research use; cells for scientific, laboratory or medical research, chemical reagents for non-medical purposes, chemical reagents, other than for medical or veterinary purposes, chemical test kits for molecular biology research for laboratory or research use, diagnostic preparations for medical-scientific research use, diagnostic reagents for medical-scientific research use, reagents for medical-scientific research use, assays and reagents for use in genetic research, biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes, chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid, diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology, nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, reagents for use in scientific apparatus for chemical or biological analysis, clinical and diagnostic reagents and cells for medical use; consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; custom design and development of chemical reagents and biochemical assays; design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; research and development in the pharmaceutical and biotechnology fields; scientific research in the field of genetics and genetic engineering.

Abstract

Methods and systems for preparing clonal cell populations are described. In some instances the disclosed methods comprise: a) identifying and selecting a cell based on its position on a surface or in a container, where the selection is not based on whether the cell comprises an exogenous label or an expressed reporter; b) photoablating all non-selected cells on the surface or in the container; and c) growing a clonal population of the selected cell.

IPC Classes  ?

• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
• G01N 1/28 - Preparing specimens for investigation
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• G01N 21/00 - Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light

Abstract

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

IPC Classes  ?

• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C07D 311/16 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 7

Abstract

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

IPC Classes  ?

• C07D 311/16 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 7
• C12N 9/22 - Ribonucleases
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12P 19/30 - Nucleotides

Goods & Services

Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; Assays for research purposes; Biochemical reagents used for non-medical purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Cells for scientific and research use; Cells for scientific, laboratory or medical research; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes; Chemical test kits for molecular biology research for laboratory or research use; Diagnostic preparations for scientific or research use other than for medical use; Diagnostic reagents for scientific use; Diagnostic reagents for scientific or research use; Reagents for research purposes; Reagents for scientific and research use; Reagents for scientific or medical research use; Assays and reagents for use in genetic research; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Reagents for use in scientific apparatus for chemical or biological analysis Reagents and cells for medical use Biochemical research and development; Biotechnology research; DNA analysis services for scientific research purposes; Genetic mapping for scientific purposes; Scientific research; Scientific research and development; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the field of genetics and genetic engineering

Goods & Services

Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics Reagents and cells for medical use

Goods & Services

Reagents, assays and cells for scientific or research use for development or manufacture of pharmaceuticals, diagnostics and therapeutics; Assays for research purposes; Biochemical reagents used for non-medical purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Cells for scientific and research use; Cells for scientific, laboratory or medical research; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes; Chemical test kits for molecular biology research for laboratory or research use; Diagnostic preparations for scientific or research use other than for medical use; Diagnostic reagents for scientific use; Diagnostic reagents for scientific or research use; Reagents for research purposes; Reagents for scientific and research use; Reagents for scientific or medical research use; Assays and reagents for use in genetic research; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Reagents for use in scientific apparatus for chemical or biological analysis Reagents and cells for medical use Biochemical research and development; Biotechnology research; DNA analysis services for scientific research purposes; Genetic mapping for scientific purposes; Scientific research; Scientific research and development; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the field of genetics and genetic engineering

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Abstract

The present disclosure provides a method for using homology directed repair to introduce an insert into a gene in order to induce knock-out of the gene. The insert can comprise a stop codon in each reading frame of the target sequence, and optionally, a restriction endonuclease recognition sequence.

IPC Classes  ?

• A01H 1/00 - Processes for modifying genotypes
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/24 - Stationary reactors without moving elements inside
• B01J 19/02 - Apparatus characterised by being constructed of material selected for its chemically-resistant properties
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Abstract

The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

IPC Classes  ?

• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

The present disclosure provides a method for determining the probability distribution of editing outcomes, such as indel length or genotype, resulting from a targeted endonuclease mediated edit, as well as a method for determining a guide RNAs or donor polynucleotides able to produce a desired editing outcome. The editing outcomes can be cell-type specific. The methods can comprise the use of machine learning models to produce the probability distributions.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 15/90 - Stable introduction of foreign DNA into chromosome

Abstract

Provided are automated systems and methods for synthesis of biopolymers including DNA and RNA. The system comprises: a reagent supply system; a first manifold coupled to the reagent supply system; a plurality of reaction vessels coupled to the first manifold, where each reaction vessel of the plurality of reaction vessels comprises a first porous membrane, a second porous membrane, and a region disposed between the first porous membrane and second porous membrane for containing a solid support medium; a reagent control system coupled to the plurality of vessels, wherein the reagent control system is configured to selectively deliver reagent originating from the reagent supply system to a first subset of the plurality of reaction vessels; and an outlet port coupled to the first manifold; wherein the system is configured such that reagent from the reagent supply system passes through the system to the outlet port without contacting the ambient environment.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01J 14/00 - Chemical processes in general for reacting liquids with liquidsApparatus specially adapted therefor
• C08F 2/01 - Processes of polymerisation characterised by special features of the polymerisation apparatus used
• C08G 85/00 - General processes for preparing compounds provided for in this subclass
• C08G 101/00 - Manufacture of cellular products
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Abstract

The present invention provides an automated modular system and method for production of biopolymers including DNA and RNA. The system and method automates the complete production process for biopolymers. Modular equipment is provided for performing production steps with the individual modules arrange in a linear array. Each module includes a control system and can be rack mounted. One side of the array of modules provides connections for power, gas, vacuum and reagents and is accessible to technicians. On the other side of the array of modules a robotic transport system is provided for transporting materials between module interfaces. The elimination of the requirement for human intervention at multiple steps in the production process significantly decreases the costs of biopolymer production and reduces unnecessary complexity and sources of quality variation.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• G05B 19/418 - Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM]

Goods & Services

Assays and reagents for use in genetic research; assays for research purposes; biochemical reagents used for non-medical purposes; biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; biochemicals, namely, precursors for in vitro genetic engineering use; cells for scientific and research use; cells for scientific, laboratory or medical research; chemical reagents for non-medical purposes; chemical reagents for non-medical purposes; chemical reagents, other than for medical or veterinary purposes; chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; chemical test kits for molecular biology research for laboratory or research use; diagnostic preparations for scientific or research use other than for medical use; diagnostic reagents and preparations, except for medical or veterinary use; diagnostic reagents and preparations, except for medical or veterinary use; diagnostic reagents for scientific use; diagnostic reagents for scientific or research use; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; diagnostic preparations for scientific or research use other than for medical use; nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; nucleotides for scientific and research use; reagent kits comprising generic dna circle, dna primers, polymerase and buffers for use in biotechnology fields; reagents for scientific or medical research use; reagents for use in scientific apparatus for chemical or biological analysis. Biochemical research and development; biotechnology research; consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; custom design and development of chemical reagents and biochemical assays; design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; dna analysis services for scientific research purposes; dna screening for scientific research purposes; genetic mapping for scientific purposes; genetic testing for scientific research purposes; genetic testing of laboratory animals for research purposes; providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; research and development in the pharmaceutical and biotechnology fields; scientific research; scientific research and development; scientific research in the field of genetics and genetic engineering.

Goods & Services

Assays for research purposes; biochemical reagents used for non-medical purposes; biochemicals, namely, precursors for in vitro genetic engineering use; cells for scientific and research use; cells for scientific, laboratory or medical research; chemical reagents for non-medical purposes; chemical reagents, other than for medical or veterinary purposes; chemical test kits for molecular biology research for laboratory or research use; diagnostic preparations for scientific or research use other than for medical use; diagnostic reagents for scientific use; diagnostic reagents for scientific or research use; nucleotides for scientific and research use; reagents for research purposes; reagents for scientific and research use; reagents for scientific or medical research use; assays and reagents for use in genetic research; biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; diagnostic reagents and preparations, except for medical or veterinary use; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; reagents for use in scientific apparatus for chemical or biological analysis. Biochemical research and development; biotechnology research; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; genetic mapping for scientific purposes; genetic testing for scientific research purposes; genetic testing of laboratory animals for research purposes; scientific research; scientific research and development; consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; custom design and development of chemical reagents and biochemical assays; design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; research and development in the pharmaceutical and biotechnology fields; scientific research in the field of genetics and genetic engineering.

Goods & Services

(1) Assays for use in scientific research; Biochemical reagents, namely, oligonucleotides, polynucleotides, probes, primers, enzymes, buffers and solvents, used for non-medical purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Stem cells, namely induced pluripotent stem cells, primary cells and immortalized cell lines, for scientific and research use; Stem cells, namely induced pluripotent stem cells, primary cells and immortalized cell lines, for scientific, laboratory or medical research; Chemical reagents for non-medical purposes, namely scientific research; Chemical reagents, other than for medical or veterinary purposes, namely scientific research; Chemical test kits for molecular biology research for laboratory and research use; Diagnostic preparations for use other than for medical use, namely scientific research; Diagnostic reagents for scientific use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Diagnostic reagents for scientific and research use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Nucleotides for scientific and research use; Reagents for research purposes, namely scientific research, medical research, agricultural research and veterinary research purposes; Reagents for scientific and research use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Reagents for scientific and medical research use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Biochemical assays and reagents for use in genetic research ; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, namely scientific research purposes; Reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; Reagents for use in scientific apparatus to isolate cells for chemical and biological analysis (1) Biochemical research and development of reagents, assays, and services related to genomic engineering; Biotechnology research; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; Genetic mapping for scientific purposes; Genetic testing for scientific research purposes; Genetic testing of laboratory animals for research purposes; Scientific research in the life sciences field; Scientific research and development in the life sciences field; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, chemical engineering, genetic engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and customization of chemical reagents and biochemical reagents for medical, scientific, and technological applications; Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the field of genetics and genetic engineering

Goods & Services

(1) Biochemical assays and reagents for use in genetic research; Assays for use in scientific research; Biochemical reagents, namely, oligonucleotides, polynucleotides, probes, primers, enzymes, buffers and solvents used for non-medical purposes; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Stem cells, namely induced pluripotent stem cells, primary cells and immortalized cell lines for scientific and research use; Stem cells, namely induced pluripotent stem cells, primary cells and immortalized cell lines for scientific, laboratory or medical research; Chemical reagents for non-medical purposes, namely scientific research; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes, namely scientific research; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Chemical test kits for molecular biology research for laboratory and research use; Diagnostic preparations for scientific or research use other than for medical use, namely scientific research; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for scientific use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Diagnostic reagents for scientific and research use , namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Diagnostic preparations for scientific or research use other than for medical use; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes, namely scientific research purposes; Nucleotides for scientific and research use; Reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; Reagents for scientific and medical research use, namely scientific research use, medical-scientific and veterinary-scientific research use, clinical laboratory use, medical laboratory use, veterinary laboratory use, medical diagnostic use, veterinary diagnostic use, veterinary purposes, and for testing of body fluids; Reagents for use in scientific apparatus to isolated cells for chemical and biological analysis (1) Research and development of reagents, assays, and services related to genomic engineering; Biotechnology research; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, chemical engineering, genetic engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and customization of chemical reagents and biochemical reagents for medical, scientific, and technological applications; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; Genetic mapping for scientific purposes; Genetic testing for scientific research purposes; Genetic testing of laboratory animals for research purposes; Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the life sciences field; Scientific research and development in the life sciences field; Scientific research in the field of genetics and genetic engineering

Goods & Services

Assays and reagents for use in genetic research; Assays for research purposes; Biochemical reagents used for non-medical purposes; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Cells for scientific and research use; Cells for scientific, laboratory or medical research; Chemical reagents for non-medical purposes; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Chemical test kits for molecular biology research for laboratory or research use; Diagnostic preparations for scientific or research use other than for medical use; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for scientific use; Diagnostic reagents for scientific or research use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Diagnostic preparations for scientific or research use other than for medical use; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Nucleotides for scientific and research use; Reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; Reagents for scientific or medical research use; Reagents for use in scientific apparatus for chemical or biological analysis Biochemical research and development; Biotechnology research; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; Genetic mapping for scientific purposes; Genetic testing for scientific research purposes; Genetic testing of laboratory animals for research purposes; Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; Research and development in the pharmaceutical and biotechnology fields; Scientific research; Scientific research and development; Scientific research in the field of genetics and genetic engineering

Goods & Services

Assays for research purposes; Biochemical reagents used for non-medical purposes; Biochemicals, namely, precursors for in vitro genetic engineering use; Cells for scientific and research use; Cells for scientific, laboratory or medical research; Chemical reagents for non-medical purposes; Chemical reagents, other than for medical or veterinary purposes; Chemical test kits for molecular biology research for laboratory or research use; Diagnostic preparations for scientific or research use other than for medical use; Diagnostic reagents for scientific use; Diagnostic reagents for scientific or research use; Nucleotides for scientific and research use; Reagents for research purposes; Reagents for scientific and research use; Reagents for scientific or medical research use; Assays and reagents for use in genetic research; Biochemicals, namely, polynucleotides for in vitro and in vivo scientific and research purposes; Chemical solutions and preparations consisting of pre-mixed reactants and reagents for scientific and research use in connection with amplification, analysis or labeling of nucleic acid; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; Reagents for use in scientific apparatus for chemical or biological analysis Biochemical research and development; Biotechnology research; DNA analysis services for scientific research purposes; DNA screening for scientific research purposes; Genetic mapping for scientific purposes; Genetic testing for scientific research purposes; Genetic testing of laboratory animals for research purposes; Scientific research; Scientific research and development; Consulting services in the fields of biotechnology, pharmaceutical research and development and genetic science; Consulting services in the fields of biotechnology, pharmaceutical research and development, laboratory testing, diagnostics, and pharmacogenetics; Custom design and development of chemical reagents and biochemical assays; Design, engineering, research, development and testing services in the field of polymeric materials, polymer synthesis, and materials customization for medical, scientific, and technological applications; Providing reagent sample testing and diagnostic services for others in the fields of science and research related thereto; Research and development in the pharmaceutical and biotechnology fields; Scientific research in the field of genetics and genetic engineering