An automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/‘order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. The sample inoculation assembly includes a module that permits a slide to be advanced out of the inoculation module to an operator for expedited processing which module also includes a printer for applying a machine readable code to the slide to associate the slide with the sample to be inoculated onto the slide. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior. Friction pads situated upon the interior surface of each vertical element are brought into contact with the exterior of the container, thereby gripping it. This gripping action holds the container with sufficient friction to permit the removal or attachment of a screw cap. Further embodiments of the invention include a mechanically biased platform supporting the channel and the pivoting levers. This base is biased and positioned to permit the channel and the pivoting lever assembly to be translated downward against the force biasing the platform and translate through the body of the container restraint. This further advancement of container, the channel and the lever assembly cause the pivoting levers to assume fully engaged gripping positions, and brings the vertical elements of the levers (and flexible friction pads upon them) into full upright positions. In this position the friction pads apply a maximum static friction force to the exterior of the container.
An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.
A blood metering device for determining an accurate target fill volume of blood in a collection vessel is disclosed herein. The blood metering device includes a housing having an inlet and an outlet, a blood flow conduit defined in the housing for providing a continuous conduit from the inlet to the outlet, and a valve disposed in the blood flow conduit. A valve operation is controlled by a valve actuator for moving the valve from the open position to the closed position. The valve actuator is responsive to a measured gas pressure in a collection vessel in fluid communication with the outlet of the housing where the valve is moved to the closed position when the measured gas pressure is approximately equal to a target gas pressure. A method for determining an accurate target fill volume of blood in a collection vessel is also disclosed herein.
Disclosed herein are method, system, and computer program product embodiments for calibrating an actuator. For example, the method may include: controlling an electronically commutated motor to drive an actuator member of the actuator away from a mechanical stop until a reference sensor is triggered by the actuator member; determining a position of the electronically commutated motor at which the actuator member of the actuator triggered the reference sensor; controlling the electronically commutated motor to repeatedly reciprocate the actuator member varying search distances from the position toward the mechanical stop and back to triggering the reference sensor until two reciprocated search distances from the position, which are at least a portion of a commutation step of the electronically commutated motor apart, are detected; and determining, based on the two reciprocated search distances, a distance for accurately positioning the actuator member relative to the mechanical stop.
An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
A system that may automatically and rapidly process blood culture bottles. Such processing includes obtaining an image of a label placed on the cylindrical surface of the blood culture bottle. The system may also determine an amount of blood sample that has been added to the blood culture bottle by comparing a sample level to pre-placed fiducial. The distance between the liquid meniscus in the blood culture bottle and fiducial may be used to determine blood volume. The imaging apparatus may also detect internal conditions of the blood culture bottle such as the presence of foam or the presence of culture media in a neck portion of the blood culture bottle. The image of the label is obtained from one or more images captured by a camera or scanner. The information on the label may be read automatically and therefore the blood culture bottles do not have to be processed manually, increasing throughput of the apparatus.
G06V 10/14 - Optical characteristics of the device performing the acquisition or on the illumination arrangements
G06V 10/22 - Image preprocessing by selection of a specific region containing or referencing a pattern; Locating or processing of specific regions to guide the detection or recognition
G06V 10/25 - Determination of region of interest [ROI] or a volume of interest [VOI]
8.
METHOD AND SYSTEM FOR AUTOMATED MICROBIAL COLONY COUNTING FROM STREAKED SAMPLE ON PLATED MEDIA
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
A system for capturing an image of a plated culture dish. The system includes an imaging device having a camera with a telecentric lens adapted to capture an image of the plated culture dish, a minor adapted to ensure that a label on the side of the plated culture dish is captured in an image of the plated culture dish that is captured by the imaging device. The system further includes at least one light system for illuminating the plated culture dish for image capture. The mirror is placed adjacent to the side of the plated culture dish on which the label is placed and at least a portion of the minor extends beneath a bottom portion of the plated culture dish at the side of the plated culture dish.
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
An automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/'order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. The sample inoculation assembly includes a module that permits a slide to be advanced out of the inoculation module to an operator for expedited processing which module also includes a printer for applying a machine readable code to the slide to associate the slide with the sample to be inoculated onto the slide. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.
The present disclosure describes automated systems and methods for handling objects, such as culture plates or dishes. For example, in one embodiment, the present disclosure describes an automated stacker and de-stacker comprising a clamping mechanism, a lift pad, a pair of pins, and a cabinet. A stack of culture plates may be stored in the cabinet. During a stacking operation, the pair of pins may be raised to stop a culture plate traveling along a conveyor track. Once stopped, the culture plate may be raised above the conveyor track by the lift pad and clamped by the clamping mechanism. During a de-stacking operation, the clamping mechanism may be opened, and the culture plate may be lowered onto the conveyor track by the lift pad.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
A blood metering device for determining an accurate target fill volume of blood in a collection vessel is disclosed herein. The blood metering device includes a housing having an inlet and an outlet, a blood flow conduit defined in the housing for providing a continuous conduit from the inlet to the outlet, and a valve disposed in the blood flow conduit. A valve operation is controlled by a valve actuator for moving the valve from the open position to the closed position. The valve actuator is responsive to a measured gas pressure in a collection vessel in fluid communication with the outlet of the housing where the valve is moved to the closed position when the measured gas pressure is approximately equal to a target gas pressure. A method for determining an accurate target fill volume of blood in a collection vessel is also disclosed herein.
A61B 5/153 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes
A61B 5/154 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes using pre-evacuated means
A61B 5/157 - Devices for taking samples of blood characterised by integrated means for measuring characteristics of blood
A blood collection system for determining an accurate blood fill volume in a collection vessel is disclosed herein. The blood collection system includes a flow component and a blood metering device connected to the flow component. The flow component comprises a flow channel having a T-junction. The blood metering device includes a control unit for operating the blood metering device, a barrel configured to align with a neck of a. collection vessel for receiving the neck therewithin. and an adapter including a luer connector at a first end portion thereof for coupling with the control unit and a second end portion thereof for coupling with the barrel. A pressure sensor is disposed in a channel adjacent to and in fluid communication with the T-junction. Methods of determining an accurate blood fill volume in a collection vessel are also disclosed herein.
A61B 90/00 - Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups , e.g. for luxation treatment or for protecting wound edges
A blood metering device for determining an accurate target fill volume of blood in a collection vessel is disclosed herein. The blood metering device includes a housing having an inlet and an outlet, a blood flow conduit defined in the housing for providing a continuous conduit from the inlet to the outlet, and a valve disposed in the blood flow conduit. A valve operation is controlled by a valve actuator for moving the valve from the open position to the closed position. The valve actuator is responsive to a measured gas pressure in a collection vessel in fluid communication with the outlet of the housing where the valve is moved to the closed position when the measured gas pressure is approximately equal to a target gas pressure. A method for determining an accurate target fill volume of blood in a collection vessel is also disclosed herein.
A61B 5/153 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes
A61B 5/154 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes using pre-evacuated means
A61B 5/157 - Devices for taking samples of blood characterised by integrated means for measuring characteristics of blood
A blood collection system for determining an accurate blood fill volume in a collection vessel is disclosed herein. The blood collection system includes a flow component and a blood metering device connected to the flow component. The flow component comprises a flow channel having a T-junction. The blood metering device includes a control unit for operating the blood metering device, a barrel configured to align with a neck of a. collection vessel for receiving the neck therewithin. and an adapter including a luer connector at a first end portion thereof for coupling with the control unit and a second end portion thereof for coupling with the barrel. A pressure sensor is disposed in a channel adjacent to and in fluid communication with the T-junction. Methods of determining an accurate blood fill volume in a collection vessel are also disclosed herein.
A61B 90/00 - Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups , e.g. for luxation treatment or for protecting wound edges
17.
Multiple carousel cartridge-based dispensing system and method
An improved system and method for the selection and dispensing of reagents onto a target testing medium in an automated laboratory environment. The system utilizes multiple carousels rotatably-mounted upon a central turntable. The position of the central turntable, as well as the rotation of each carousel is controlled by a microprocessor-based control system. Multiple dispensers, each capable of storing and dispensing a particular reagent, are mounted about the circumference of each carousel. This configuration provides much higher reagent density than previously available. The testing medium is positioned at one or more loading stations. The control system directs the central turntable to a position from which a selected one of the carousels can be rotated so as to position a selected dispenser above the testing medium. The selected dispenser is then actuated to release a predetermined reagent onto the testing medium. The system can be configured so that multiple reagents can be dispensed, each from an associated dispenser, onto a single or multiple testing mediums. Each of these actuated dispensers being positioned over the targeted testing medium(s) positioned at one or more loading stations.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
G06T 7/90 - Determination of colour characteristics
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G06T 3/00 - Geometric image transformation in the plane of the image
An apparatus that configures a culture plate for precisely assigning coordinates to a selected colony by assigning fiducials to the culture plate using simple mechanical techniques. The fiducials correspond to the plate center and the center of a barcode label applied to the side of the culture plate by the apparatus. The apparatus then deploys mechanisms to apply coordinates to colonies identified by the user relative to the fiducials. One such mechanism is a webcam pointed at the culture plate that allows a technician to mark the colonies on a display using a computer mouse or equivalent cursor. Another mechanism deploys a laser pointer directed at the colony wherein the apparatus assigns coordinates to the location of the colony onto which the laser pointer is directed. In a third mechanism, the user views the surface of the culture plate through a viewfinder and manually registers coordinates when the view finder cross hairs are over a target colony. The selected colonies are assigned precise coordinates with reference to the two fiducials. Software is provided such that pixels in the image of the culture plate correspond to the coordinates of the culture dish relative to the fiducials.
A system that may automatically and rapidly process blood culture bottles. Such processing includes obtaining an image of a label placed on the cylindrical surface of the blood culture bottle. The system may also determine an amount of blood sample that has been added to the blood culture bottle by comparing a sample level to pre-placed fiducial. The distance between the liquid meniscus in the blood culture bottle and fiducial may be used to determine blood volume. The imaging apparatus may also detect internal conditions of the blood culture bottle such as the presence of foam or the presence of culture media in a neck portion of the blood culture bottle. The image of the label is obtained from one or more images captured by a camera or scanner. The information on the label may be read automatically and therefore the blood culture bottles do not have to be processed manually, increasing throughput of the apparatus.
C12M 1/24 - Apparatus for enzymology or microbiology tube or bottle type
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G01N 21/84 - Systems specially adapted for particular applications
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
21.
DISPOSABLE DEVICE FOR VENTING A SEALED CONTAINER AND ALIQUOTING THEREFROM
A multi-port disposable device that can both vent a sealed culture vessel and draw a sample therefrom into a collection vessel. The multi-port disposable device has a first port configured to receive the top portion of a culture vessel and attach thereto. The multi-port disposable device has a second port configured to receive a sample collection vessel. The multi-port disposable device has first and second needles. The first needle has a cannula configured to penetrate a septum or cap of the culture vessel. Optionally, the cannula terminates in a layer of foam that carries a microbial agent such that any vapor that is transmitted from the culture vessel through the cannula when venting the sealed culture vessel is absorbed by the foam. Optionally, the ports of the disposable device are configured as sleeves. The sleeve of the first port receives a portion of the culture vessel and the sleeve of the second port receives the collection vessel. The second needle has a cannula that penetrates both the septum or cap of the culture vessel and the septum or cap of the collection vessel, when the culture vessel and the collection vessel are brought into the device, thereby providing for fluid communication between the culture vessel and the collection vessel. The second cannula provides the flow pathway for delivering sample from the culture vessel to the collection vessel.
Measurement system which can be used at patients bedside to monitor the amount of blood drawn from the patient. The system uses disposable sensor and electronics to measure accurately and in real time the volume of the blood drawn from the patient using a paddlewheel sensor wherein the rotation of the paddle wheel is correlated with the volume of the blood that is drawn from the patient and collected.
A61B 5/154 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes using pre-evacuated means
A system that may automatically and rapidly process blood culture bottles. Such processing includes obtaining an image of a label placed on the cylindrical surface of the blood culture bottle. The system may also determine an amount of blood sample that has been added to the blood culture bottle by comparing a sample level to pre-placed fiducial. The distance between the liquid meniscus in the blood culture bottle and fiducial may be used to determine blood volume. The imaging apparatus may also detect internal conditions of the blood culture bottle such as the presence of foam or the presence of culture media in a neck portion of the blood culture bottle. The image of the label is obtained from one or more images captured by a camera or scanner. The information on the label may be read automatically and therefore the blood culture bottles do not have to be processed manually, increasing throughput of the apparatus.
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 21/84 - Systems specially adapted for particular applications
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/24 - Apparatus for enzymology or microbiology tube or bottle type
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
24.
Methods and systems for automated assessment of antibiotic sensitivity
An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
A system for capturing an image of a plated culture dish. The system includes an imaging device having a camera with a telecentric lens adapted to capture an image of the plated culture dish, a mirror adapted to ensure that a label on the side of the plated culture dish is captured in an image of the plated culture dish that is captured by the imaging device. The system further includes at least one light system for illuminating the plated culture dish for image capture. The mirror is placed adjacent to the side of the plated culture dish on which the label is placed and at least a portion of the mirror extends beneath a bottom portion of the plated culture dish at the side of the plated culture dish.
A system for capturing an image of a plated culture dish. The system includes an imaging device having a camera with a telecentric lens adapted to capture an image of the plated culture dish, a mirror adapted to ensure that a label on the side of the plated culture dish is captured in an image of the plated culture dish that is captured by the imaging device. The system further includes at least one light system for illuminating the plated culture dish for image capture. The mirror is placed adjacent to the side of the plated culture dish on which the label is placed and at least a portion of the mirror extends beneath a bottom portion of the plated culture dish at the side of the plated culture dish.
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
A holder for consumables for use in an analyzer is disclosed. The holder has one or more receptacles for receiving a stack of consumables. The consumables are wrapped in packaging and are placed in the holder as a stack of packaged consumables. The receptacles have a height sufficient to receive the stack of consumable. The receptacles have an opening miming along the side of the receptacle for the entire portion of the height of the receptacle that receives the stack of consumables. The receptacles have a proximal end and a distal end. The proximal end is the end of the receptacles into which the stack of consumables is place and from which the consumables are removed. The distal portion of the receptacles support the stack of consumables in the receptacles.
B65B 69/00 - Unpacking of articles or materials, not otherwise provided for
B65B 7/28 - Closing semi-rigid or rigid containers or receptacles not deformed by, or not taking-up shape of, contents, e.g. boxes or cartons by applying separate preformed closures, e.g. lids, covers
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
29.
Multiple carousel cartridge-based dispensing system and method
An improved system and method for the selection and dispensing of reagents onto a target testing medium in an automated laboratory environment. The system utilizes multiple carousels rotatably-mounted upon a central turntable. The position of the central turntable, as well as the rotation of each carousel is controlled by a microprocessor-based control system. Multiple dispensers, each capable of storing and dispensing a particular reagent, are mounted about the circumference of each carousel. This configuration provides much higher reagent density than previously available. The testing medium is positioned at one or more loading stations. The control system directs the central turntable to a position from which a selected one of the carousels can be rotated so as to position a selected dispenser above the testing medium. The selected dispenser is then actuated to release a predetermined reagent onto the testing medium. The system can be configured so that multiple reagents can be dispensed, each from an associated dispenser, onto a single or multiple testing mediums. Each of these actuated dispensers being positioned over the targeted testing medium(s) positioned at one or more loading stations.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior. Friction pads situated upon the interior surface of each vertical element are brought into contact with the exterior of the container, thereby gripping it. This gripping action holds the container with sufficient friction to permit the removal or attachment of a screw cap. Further embodiments of the invention include a mechanically biased platform supporting the channel and the pivoting levers. This base is biased and positioned to permit the channel and the pivoting lever assembly to be translated downward against the force biasing the platform and translate through the body of the container restraint. This further advancement of container, the channel and the lever assembly cause the pivoting levers to assume fully engaged gripping positions, and brings the vertical elements of the levers (and flexible friction pads upon them) into full upright positions. In this position the friction pads apply a maximum static friction force to the exterior of the container.
An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.
The present disclosure describes automated systems and methods for handling objects, such as culture plates or dishes. For example, in one embodiment, the present disclosure describes an automated stacker and de-stacker comprising a clamping mechanism, a lift pad, a pair of pins, and a cabinet. A stack of culture plates may be stored in the cabinet. During a stacking operation, the pair of pins may be raised to stop a culture plate traveling along a conveyor track. Once stopped, the culture plate may be raised above the conveyor track by the lift pad and clamped by the clamping mechanism. During a de-stacking operation, the clamping mechanism may be opened, and the culture plate may be lowered onto the conveyor track by the lift pad.
The present disclosure describes automated systems and methods for handling objects, such as culture plates or dishes. For example, in one embodiment, the present disclosure describes an automated stacker and de-stacker comprising a clamping mechanism, a lift pad, a pair of pins, and a cabinet. A stack of culture plates may be stored in the cabinet. During a stacking operation, the pair of pins may be raised to stop a culture plate traveling along a conveyor track. Once stopped, the culture plate may be raised above the conveyor track by the lift pad and clamped by the clamping mechanism. During a de-stacking operation, the clamping mechanism may be opened, and the culture plate may be lowered onto the conveyor track by the lift pad.
The present disclosure describes systems and methods for centering a circular object, such as a petri dish, between a plurality of pins. For example, in one embodiment, a method comprises placing a circular object on a rotatable platform surrounded by three moveable pins. In order to roughly center the circular object, the method further comprises moving, for a first time, all of the pins toward the circular object until at least two out of the three pins are touching the circular object. In order to more accurately center the circular object, the method further comprises: moving all of the pins away from the circular object so that it can be rotated without substantial inference; rotating the platform approximately 60 degrees, wherein rotating the platform causes the circular object to also rotate by approximately 60 degrees; and moving, for a second time, all of the pins toward the circular object.
The present invention describes an integrated incubator and image capture module that regulates the incubator atmosphere and obtains high-resolution digital images of sample specimens. The incubator has a cabinet type enclosure that enables the provision of a controlled environment to the contents of the incubator by having at least three ports on one face of the cabinet for the passage of sample containers. Additionally, an image capture module is located immediately adjacent to the incubator. In this regard, using at least three separate access/egress points for the sample containers streamlines operation of the system and enhances preservation of the incubator environment. Furthermore, locating the image capture module directly adjacent to the incubator reduces the amount of time a sample container is exposed to the external environment, thereby reducing the extent to which samples are exposed to potential contaminants and reducing the exchange of the lab and ambient atmospheres.
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
37.
SYSTEM AND METHOD FOR THE AUTOMATED PREPARATION OF BIOLOGICAL SAMPLES
The present invention describes an automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
38.
Method for direct inoculation of a broth from a source suspension
An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.
A system and method for gripping, torqueing and releasing an element so as to cap and/or decap a container, such as those typically utilized to house specimens in laboratory environments. The system is driven by a single bi-directional motor linked to a coupler assembly via a rotating threaded shaft. The coupler assembly is configured to engage with an element, such as a cap or container, via mechanically-biased splines that are actuated without any complex linkages, or operative connection to the motor or other powered components. The system employs an ejector nut and an ejector, both of which are concentrically positioned about the threaded shaft. The ejector nut translates along the shaft as a function of the shaft's rotation, so as to permit the retraction of the ejector when an element is engaged in the coupler assembly or cause the ejector to extend into the coupler assembly to disengage the element. The direction and rotation of the motor is controlled by a system coupled to sensors positioned within the system. Such control system may include one or more processors, component interfaces, and data storage/memory. The sensors may include multiple optical, magnetic or mechanical means for monitoring one or more of the positions of the ejector nut and ejector along the threaded shaft, and/or the rotational position of the coupler assembly.
B67B 7/15 - Hand- or power-operated devices for opening closed containers for removing tightly-fitting lids or covers, e.g. of shoe-polish tins, by gripping and rotating finger grapple type
B67B 7/18 - Hand- or power-operated devices for opening closed containers for removing threaded caps
G01B 11/26 - Measuring arrangements characterised by the use of optical techniques for testing the alignment of axes
G01D 5/20 - Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying inductance, e.g. by a movable armature
40.
AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS
A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provide for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
41.
Automated selection of microorganisms and identification using MALDI
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
Measurement system which can be used at patient's bedside to monitor the amount of blood drawn from the patient. The system uses disposable sensor and electronics to measure accurately and in real time the volume of the blood drawn from the patient using a paddlewheel sensor wherein the rotation of the paddle wheel is correlated with the volume of the blood that is drawn from the patient and collected.
A61B 5/153 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes
A61B 5/154 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes using pre-evacuated means
43.
DISPOSABLE DEVICE FOR VENTING A SEALED CONTAINER AND ALIQUOTING THEREFROM
A multi-port disposable device that can both vent a sealed culture vessel and draw a sample therefrom into a collection vessel. The multi-port disposable device has a first port configured to receive the top portion of a culture vessel and attach thereto. The multi-port disposable device has a second port configured to receive a sample collection vessel. The multi-port disposable device has first and second needles. The first needle has a cannula configured to penetrate a septum or cap of the culture vessel. Optionally, the cannula terminates in a layer of foam that carries a microbial agent such that any vapor that is transmitted from the culture vessel through the cannula when venting the sealed culture vessel is absorbed by the foam. Optionally, the ports of the disposable device are configured as sleeves. The sleeve of the first port receives a portion of the culture vessel and the sleeve of the second port receives the collection vessel. The second needle has a cannula that penetrates both the septum or cap of the culture vessel and the septum or cap of the collection vessel, when the culture vessel and the collection vessel are brought into the device, thereby providing for fluid communication between the culture vessel and the collection vessel. The second cannula provides the flow pathway for delivering sample from the culture vessel to the collection vessel.
Measurement system which can be used at patient's bedside to monitor the amount of blood drawn from the patient. The system uses disposable sensor and electronics to measure accurately and in real time the volume of the blood drawn from the patient using a paddlewheel sensor wherein the rotation of the paddle wheel is correlated with the volume of the blood that is drawn from the patient and collected.
A61B 5/153 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes
A61B 5/154 - Devices for taking samples of blood specially adapted for taking samples of venous or arterial blood, e.g. by syringes using pre-evacuated means
45.
DISPOSABLE DEVICE FOR VENTING A SEALED CONTAINER AND ALIQUOTING THEREFROM
A multi-port disposable device that can both vent a sealed culture vessel and draw a sample therefrom into a collection vessel. The multi-port disposable device has a first port configured to receive the top portion of a culture vessel and attach thereto. The multi-port disposable device has a second port configured to receive a sample collection vessel. The multi-port disposable device has first and second needles. The first needle has a cannula configured to penetrate a septum or cap of the culture vessel. Optionally, the cannula terminates in a layer of foam that carries a microbial agent such that any vapor that is transmitted from the culture vessel through the cannula when venting the sealed culture vessel is absorbed by the foam. Optionally, the ports of the disposable device are configured as sleeves. The sleeve of the first port receives a portion of the culture vessel and the sleeve of the second port receives the collection vessel. The second needle has a cannula that penetrates both the septum or cap of the culture vessel and the septum or cap of the collection vessel, when the culture vessel and the collection vessel are brought into the device, thereby providing for fluid communication between the culture vessel and the collection vessel. The second cannula provides the flow pathway for delivering sample from the culture vessel to the collection vessel.
An apparatus that configures a culture plate for precisely assigning coordinates to a selected colony by assigning fiducials to the culture plate using simple mechanical techniques. The fiducials correspond to the plate center and the center of a barcode label applied to the side of the culture plate by the apparatus. The apparatus then deploys mechanisms to apply coordinates to colonies identified by the user relative to the fiducials. One such mechanism is a webcam pointed at the culture plate that allows a technician to mark the colonies on a display using a computer mouse or equivalent cursor. Another mechanism deploys a laser pointer directed at the colony wherein the apparatus assigns coordinates to the location of the colony onto which the laser pointer is directed. In a third mechanism, the user views the surface of the culture plate through a viewfinder and manually registers coordinates when the view finder cross hairs are over a target colony. The selected colonies are assigned precise coordinates with reference to the two fiducials. Software is provided such that pixels in the image of the culture plate correspond to the coordinates of the culture dish relative to the fiducials.
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
47.
METHOD AND SYSTEM FOR LOCATING A BACTERIAL COLONY ON A CULTURE PLATE
An apparatus that configures a culture plate for precisely assigning coordinates to a selected colony by assigning fiducials to the culture plate using simple mechanical techniques. The fiducials correspond to the plate center and the center of a barcode label applied to the side of the culture plate by the apparatus. The apparatus then deploys mechanisms to apply coordinates to colonies identified by the user relative to the fiducials. One such mechanism is a webcam pointed at the culture plate that allows a technician to mark the colonies on a display using a computer mouse or equivalent cursor. Another mechanism deploys a laser pointer directed at the colony wherein the apparatus assigns coordinates to the location of the colony onto which the laser pointer is directed. In a third mechanism, the user views the surface of the culture plate through a viewfinder and manually registers coordinates when the view finder cross hairs are over a target colony. The selected colonies are assigned precise coordinates with reference to the two fiducials. Software is provided such that pixels in the image of the culture plate correspond to the coordinates of the culture dish relative to the fiducials.
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
48.
Methods and systems for automated assessment of antibiotic sensitivity
An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times (e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s) and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s) of the second image that differ from the simulated image.
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.
An imaging method for earliest microbial growth detection. The method uses images to determined colony biomass, and the colony biomass determines when the colony can be picked for analysis for identification or antibiotic susceptibility testing. If the sample source is not a pure sample source additional incubation may be required to permit an increase in biomass of the colonies prior to pick.
An improved system and method for the selection and dispensing of reagents onto a target testing medium in an automated laboratory environment. The system utilizes multiple carousels rotatably -mounted upon a central turntable. The position of the central turntable, as well as the rotation of each carousel is controlled by a microprocessor-based control system. Multiple dispensers, each capable of storing and dispensing a particular reagent, are mounted about the circumference of each carousel. This configuration provides much higher reagent density than previously available. The testing medium is positioned at one or more loading stations. The control system directs the central turntable to a position from which a selected one of the carousels can be rotated so as to position a selected dispenser above the testing medium. The selected dispenser is then actuated to release a predetermined reagent onto the testing medium. The system can be configured so that multiple reagents can be dispensed, each from an associated dispenser, onto a single or multiple testing mediums. Each of these actuated dispensers being positioned over the targeted testing medium(s) positioned at one or more loading stations.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
53.
MULTIPLE CAROUSEL CARTRIDGE-BASED DISPENSING SYSTEM AND METHOD
An improved system and method for the selection and dispensing of reagents onto a target testing medium in an automated laboratory environment. The system utilizes multiple carousels rotatably -mounted upon a central turntable. The position of the central turntable, as well as the rotation of each carousel is controlled by a microprocessor-based control system. Multiple dispensers, each capable of storing and dispensing a particular reagent, are mounted about the circumference of each carousel. This configuration provides much higher reagent density than previously available. The testing medium is positioned at one or more loading stations. The control system directs the central turntable to a position from which a selected one of the carousels can be rotated so as to position a selected dispenser above the testing medium. The selected dispenser is then actuated to release a predetermined reagent onto the testing medium. The system can be configured so that multiple reagents can be dispensed, each from an associated dispenser, onto a single or multiple testing mediums. Each of these actuated dispensers being positioned over the targeted testing medium(s) positioned at one or more loading stations.
G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
An apparatus for conveying a plurality of articles includes a transport belt, a bumper stopper, and a rotator. A motor moves the transport belt which is adapted to rotate the rotator as the belt conveys the plurality of articles. The stopper can move between first and second positions relative to the transport belt wherein the bumper stopper guides one article toward the rotator when the bumper stopper is in the first position. The bumper stopper allows the article to be conveyed to another location when the bumper stopper is in the second position. The transport belt can continue to convey other articles while the one article is temporarily held in place by the bumper stopper. The rotator is configured to rotate the article conveyed by the transport belt. The article can remain in contact with the transport belt while the rotator is rotating the article.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior. Friction pads situated upon the interior surface of each vertical element are brought into contact with the exterior of the container, thereby gripping it. This gripping action holds the container with sufficient friction to permit the removal or attachment of a screw cap. Further embodiments of the invention include a mechanically biased platform supporting the channel and the pivoting levers. This base is biased and positioned to permit the channel and the pivoting lever assembly to be translated downward against the force biasing the platform and translate through the body of the container restraint. This further advancement of container, the channel and the lever assembly cause the pivoting levers to assume fully engaged gripping positions, and brings the vertical elements of the levers (and flexible friction pads upon them) into full upright positions. In this position the friction pads apply a maximum static friction force to the exterior of the container.
A system and method for a self-actuating, mechanically-biased container restraint. The system requires no computer-aided control or timing, nor is any external power source needed, other than the force exerted as a container is inserted into the restraint. The system relies upon an assembly including mechanically-biased pivoting levers, each of which has a horizontal element and a vertical element. All actuation occurs as the base of an inserted container comes into contact with the upper surface of the horizontal elements of multiple pivoted levers positioned at the base of a channel adapted to serve as a guide for the inserted tube. The levers are biased in this elevated position by mechanical means, such as a spring. As the inserted tube presses the horizontal members downward, the top portions of the vertical members are pivoted inward toward the container's exterior. Friction pads situated upon the interior surface of each vertical element are brought into contact with the exterior of the container, thereby gripping it. This gripping action holds the container with sufficient friction to permit the removal or attachment of a screw cap. Further embodiments of the invention include a mechanically biased platform supporting the channel and the pivoting levers. This base is biased and positioned to permit the channel and the pivoting lever assembly to be translated downward against the force biasing the platform and translate through the body of the container restraint. This further advancement of container, the channel and the lever assembly cause the pivoting levers to assume fully engaged gripping positions, and brings the vertical elements of the levers (and flexible friction pads upon them) into full upright positions. In this position the friction pads apply a maximum static friction force to the exterior of the container.
A holder for consumables for use in an analyzer is disclosed. The holder has one or more receptacles for receiving a stack of consumables. The consumables are wrapped in packaging and are placed in the holder as a stack of packaged consumables. The receptacles have a height sufficient to receive the stack of consumable. The receptacles have an opening running along the side of the receptacle for the entire portion of the height of the receptacle that receives the stack of consumables. The receptacles have a proximal end and a distal end. The proximal end is the end of the receptacles into which the stack of consumables is place and from which the consumables are removed. The distal portion of the receptacles support the stack of consumables in the receptacles.
B65B 7/28 - Closing semi-rigid or rigid containers or receptacles not deformed by, or not taking-up shape of, contents, e.g. boxes or cartons by applying separate preformed closures, e.g. lids, covers
B65B 69/00 - Unpacking of articles or materials, not otherwise provided for
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
58.
A DEVICE FOR HOLDING ARTICLES TO REMOVE THE PACKAGING THEREFROM
A holder for consumables for use in an analyzer is disclosed. The holder has one or more receptacles for receiving a stack of consumables. The consumables are wrapped in packaging and are placed in the holder as a stack of packaged consumables. The receptacles have a height sufficient to receive the stack of consumable. The receptacles have an opening running along the side of the receptacle for the entire portion of the height of the receptacle that receives the stack of consumables. The receptacles have a proximal end and a distal end. The proximal end is the end of the receptacles into which the stack of consumables is place and from which the consumables are removed. The distal portion of the receptacles support the stack of consumables in the receptacles.
B65B 7/28 - Closing semi-rigid or rigid containers or receptacles not deformed by, or not taking-up shape of, contents, e.g. boxes or cartons by applying separate preformed closures, e.g. lids, covers
B65B 69/00 - Unpacking of articles or materials, not otherwise provided for
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
G06T 7/90 - Determination of colour characteristics
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G06K 9/62 - Methods or arrangements for recognition using electronic means
G06T 3/00 - Geometric image transformation in the plane of the image
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
The present disclosure describes systems and methods for centering a circular object, such as a petri dish, between a plurality of pins. For example, in one embodiment, a method comprises placing a circular object on a rotatable platform surrounded by three moveable pins. In order to roughly center the circular object, the method further comprises moving, for a first time,all of the pins toward the circular object until at least two out of the three pins are touching the circular object. In order to more accurately center the circular object, the method further comprises: moving all of the pins away from the circular object so that it can be rotated without substantial inference; rotating the platform approximately 60 degrees, wherein rotating the platform causes the circular object to also rotate by approximately 60 degrees; and moving, for a second time,all of the pins toward the circular object.
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
63.
SYSTEMS AND METHODS FOR CENTERING A CIRCULAR OBJECT
The present disclosure describes systems and methods for centering a circular object, such as a petri dish, between a plurality of pins. For example, in one embodiment, a method comprises placing a circular object on a rotatable platform surrounded by three moveable pins. In order to roughly center the circular object, the method further comprises moving, for a first time,all of the pins toward the circular object until at least two out of the three pins are touching the circular object. In order to more accurately center the circular object, the method further comprises: moving all of the pins away from the circular object so that it can be rotated without substantial inference; rotating the platform approximately 60 degrees, wherein rotating the platform causes the circular object to also rotate by approximately 60 degrees; and moving, for a second time,all of the pins toward the circular object.
C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
64.
METHOD FOR DIRECT INOCULATION OF A BROTH FROM A SOURCE SUSPENSION
An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.
An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.
A system and method for gripping, torqueing and releasing an element so as to cap and/or decap a container, such as those typically utilized to house specimens in laboratory environments. The system is driven by a single bi-directional motor linked to a coupler assembly via a rotating threaded shaft. The coupler assembly is configured to engage with an element, such as a cap or container, via mechanically-biased splines that are actuated without any complex linkages, or operative connection to the motor or other powered components. The system employs an ejector nut and an ejector, both of which are concentrically positioned about the threaded shaft. The ejector nut translates along the shaft as a function of the shaft's rotation, so as to permit the retraction of the ejector when an element is engaged in the coupler assembly or cause the ejector to extend into the coupler assembly to disengage the element. The direction and rotation of the motor is controlled by a system coupled to sensors positioned within the system. Such control system may include one or more processors, component interfaces, and data storage/memory. The sensors may include multiple optical, magnetic or mechanical means for monitoring one or more of the positions of the ejector nut and ejector along the threaded shaft, and/or the rotational position of the coupler assembly.
B67B 3/26 - Applications of control, warning, or safety devices in capping machinery
B67B 7/18 - Hand- or power-operated devices for opening closed containers for removing threaded caps
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
A system and method for gripping, torqueing and releasing an element so as to cap and/or decap a container, such as those typically utilized to house specimens in laboratory environments. The system is driven by a single bi-directional motor linked to a coupler assembly via a rotating threaded shaft. The coupler assembly is configured to engage with an element, such as a cap or container, via mechanically-biased splines that are actuated without any complex linkages, or operative connection to the motor or other powered components. The system employs an ejector nut and an ejector, both of which are concentrically positioned about the threaded shaft. The ejector nut translates along the shaft as a function of the shaft's rotation, so as to permit the retraction of the ejector when an element is engaged in the coupler assembly or cause the ejector to extend into the coupler assembly to disengage the element. The direction and rotation of the motor is controlled by a system coupled to sensors positioned within the system. Such control system may include one or more processors, component interfaces, and data storage/memory. The sensors may include multiple optical, magnetic or mechanical means for monitoring one or more of the positions of the ejector nut and ejector along the threaded shaft, and/or the rotational position of the coupler assembly.
B67B 3/26 - Applications of control, warning, or safety devices in capping machinery
B67B 7/18 - Hand- or power-operated devices for opening closed containers for removing threaded caps
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
68.
System and method for image acquisition using supervised high quality imaging
An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.
H04N 5/235 - Circuitry for compensating for variation in the brightness of the object
G01N 21/25 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 33/483 - Physical analysis of biological material
H04N 5/217 - Circuitry for suppressing or minimising disturbance, e.g. moire or halo in picture signal generation
69.
METHODS AND SYSTEMS FOR AUTOMATED ASSESSMENT OF ANTIBIOTIC SENSITIVITY
An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times(e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s)and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s)of the second image that differ from the simulated image.
C12Q 1/18 - Testing for antimicrobial activity of a material
G16B 5/00 - ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
G16B 45/00 - ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
70.
METHODS AND SYSTEMS FOR AUTOMATED ASSESSMENT OF ANTIBIOTIC SENSITIVITY
An imaging system and method provides automated microbial growth detection for antibiotic sensitivity testing. A processing system having an image sensor for capturing images of an inoculated culture plate having antibiotic disks disposed on the culture media captures images of the plate at separate times(e.g., first and second images). The system generates pixel characteristic data for pixels of the second image from a comparison of the first image and second image. The pixel characteristic data may be indicative of plate growth. The system may access growth modeling data concerning the antibiotic disk(s)and generate simulated image data with a growth model function. The growth model function uses the growth modeling data. The simulated image data simulates growth on the plate relative to the disk(s). The system compares the simulated image and the pixel characteristic data to identify pixel region(s)of the second image that differ from the simulated image.
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
This invention pertains to the general field of microbiology, and more specifically to transfer, inoculation and/or streaking of micro-organisms, e.g. for the purpose of obtaining individual colonies. Provided is a method for streaking a microbial sample onto a solid carrier, comprising the steps of:
a) contacting at least one ferromagnetic particle with a solid carrier, followed or preceded by providing the particle with at least part of said sample, and
b) applying a magnetic field gradient to allow for magnetically controlled motion of said particle on said surface, such that at least part of the sample is streaked onto the solid carrier. Also provided is an apparatus for carrying out such method in an (semi-)automated fashion.
A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
C12Q 1/04 - Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
76.
Method and system for automated microbial colony counting from streaked sample on plated media
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
A separating system (1) comprises a housing (60) sized to receive a stack of articles having at least one first article (12) stacked on top of a second article (10). The system (1) also includes a lifter (20) movable into a contact position adjacent a surface (13) on the at least one first article (12). The lifter (20) is adapted to apply a lifting force to the surface (13) to lift at least a portion of the first article (12) above the contact position. An extractor (40) moves the second article (10) away from the stack of articles after the at least one first article (12) has been lifted above the contact position by the lifter (20). The housing (60) prevents the at least one first article (12) from moving with the second article (10) when the second article (10) is moved by the extractor (40) and guides the stack of articles downward after the second article (10) is moved away.
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
G06T 7/90 - Determination of colour characteristics
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 33/569 - Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G06K 9/62 - Methods or arrangements for recognition using electronic means
G06T 3/00 - Geometric image transformation in the plane of the image
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
An apparatus for conveying a plurality of articles (10) includes a transport belt (6), a bumper stopper (14), and a rotator (12). A motor moves the transport belt which is adapted to rotate the rotator as the belt conveys the plurality of articles. The stopper can move between first and second positions relative to the transport belt wherein the bumper stopper guides one article toward the rotator when the bumper stopper is in the first position. The bumper stopper allows the article to be conveyed to another location when the bumper stopper is in the second position. The transport belt can continue to convey other articles while the one article is temporarily held in place by the bumper stopper. The rotator is configured to rotate the article conveyed by the transport belt. The article can remain in contact with the transport belt while the rotator is rotating the article.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.
G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
G01N 21/17 - Systems in which incident light is modified in accordance with the properties of the material investigated
H04N 5/235 - Circuitry for compensating for variation in the brightness of the object
G01N 21/25 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/27 - Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection
G01N 33/483 - Physical analysis of biological material
H04N 5/217 - Circuitry for suppressing or minimising disturbance, e.g. moire or halo in picture signal generation
86.
AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS
A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
88.
AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS
A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
A separating system (1) comprises a housing (60) sized to receive a stack of articles having at least one first article (12) stacked on top of a second article (10). The system (1) also includes a lifter (20) movable into a contact position adjacent a surface (13) on the at least one first article (12). The lifter (20) is adapted to apply a lifting force to the surface (13) to lift at least a portion of the first article (12) above the contact position. An extractor (40) moves the second article (10) away from the stack of articles after the at least one first article (12) has been lifted above the contact position by the lifter (20). The housing (60) prevents the at least one first article (12) from moving with the second article (10) when the second article (10) is moved by the extractor (40) and guides the stack of articles downward after the second article (10) is moved away.
An imaging system and method for microbial growth detection, counting or identification. One colony may be contrasted in an image that is not optimal for another type of colony. The system and method provides contrast from all available material through space (spatial differences), time (differences appearing over time for a given capture condition) and color space transformation using image input information over time to assess whether microbial growth has occurred for a given sample.
The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample; incubating the inoculated culture media; following incubation, obtaining a first image of the inoculated media at a first time; after further incubation, obtaining a second image of the inoculated media at a second time; aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.
92.
METHOD AND SYSTEM FOR AUTOMATICALLY COUNTING MICROBIAL COLONIES
The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.
The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.
An imaging system and method for microbial growth detection using differences appearing over time to assess whether microbial growth has occurred for a given sample.
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
An apparatus for conveying a plurality of articles (10) includes a transport belt (6), a bumper stopper (14), and a rotator (12). A motor moves the transport belt which is adapted to rotate the rotator as the belt conveys the plurality of articles. The stopper can move between first and second positions relative to the transport belt wherein the bumper stopper guides one article toward the rotator when the bumper stopper is in the first position. The bumper stopper allows the article to be conveyed to another location when the bumper stopper is in the second position. The transport belt can continue to convey other articles while the one article is temporarily held in place by the bumper stopper. The rotator is configured to rotate the article conveyed by the transport belt. The article can remain in contact with the transport belt while the rotator is rotating the article.
C12M 1/00 - Apparatus for enzymology or microbiology
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
An apparatus for conveying a plurality of articles (10) includes a transport belt (6), a bumper stopper (14), and a rotator (12). A motor moves the transport belt which is adapted to rotate the rotator as the belt conveys the plurality of articles. The stopper can move between first and second positions relative to the transport belt wherein the bumper stopper guides one article toward the rotator when the bumper stopper is in the first position. The bumper stopper allows the article to be conveyed to another location when the bumper stopper is in the second position. The transport belt can continue to convey other articles while the one article is temporarily held in place by the bumper stopper. The rotator is configured to rotate the article conveyed by the transport belt. The article can remain in contact with the transport belt while the rotator is rotating the article.
G01N 35/04 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations - Details of the conveyor system
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups ; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
