Multinuclear complexes and methods for preparing them are provided. The discrete multinuclear complexes include a one or more transition metals and a radioisotope having the same coordination geometry as the transition metal. A bridging ligand is coordinated to the transition metal and the radioisotope to link the transition metal and the radioisotope and pendent ligands are coordinated to each of the transition metal and the radioisotope to stabilise the complex. The multinuclear complexes may include a radioisotope or radioelement that can be detected by medical equipment and may find use in therapy and/or the diagnosis of disease in patients.
The present invention relates to a process for the microbial in-chain hydroxylation of C12 to C16 fatty acids, alcohols and alkanes at position ω-7, the process including the use of a microorganism expressing a cytochrome P450 monooxygenase CYP505E3 or related fungal cytochrome P450 monooxygenases sharing at least 70% amino acid identity in the production of a hydroxylated product or secondary product. The present invention further relates to a process for the preparation of lactones, esters and polymers by hydroxylation of the corresponding fatty acids, fatty alcohols and alkane precursors by a recombinant cytochrome P450 monooxygenase CYP505E3 or related fungal cytochrome P450 monooxygenases sharing at least 70% amino acid identity.
Multinuclear complexes and methods for preparing them are provided. The discrete multinuclear complexes include a one or more transition metals and a radioisotope having the same coordination geometry as the transition metal. A bridging ligand is coordinated to the transition metal and the radioisotope to link the transition metal and the radioisotope and pendent ligands are coordinated to each of the transition metal and the radioisotope to stabilise the complex. The multinuclear complexes may include a radioisotope or radioelement that can be detected by medical equipment and may find use in therapy and/or the diagnosis of disease in patients.
3) dispersed alkaline substrate (BDAS) for use in the remediation or at least partial remediation of mine drainage (MD) and/or environmental media contaminated with a source of MD. The invention utilizes chemical, biological and combined treatment systems remove high concentrations of sulfates, hardness, heavy metals and N-compounds, that may exist in the MD as well as high concentrations of alkalinity produced during the remediation process. The invention further extends to a process for treating MD and/or environmental media contaminated with MD and to an apparatus for use in this process.
The present invention relates to a process for the microbial in-chain hydroxylation of C12 to C16 fatty acids, alchohols and alkanes at position ω-7, the process including the use of a microorganism expressing a cytrochrome P450 monooxygenase CYP505E3 or related fungal cytochrome P450 monooxygenases sharing at least 70 % amino acid identity in the production of a hydroxylated product or secondary product. The present invention further relates to a process for the preparation of lactones, esters and polymers by hydroxylation of the corresponding fatty acids, fatty alcohols and alkane precursors by a recombinant cytochrome P450 monooxygenase CYP505E3 or related fungal cytochrome P450 monooxygenases sharing at least 70 % amino acid identity.
Yarrowia lipolytica yeast cell, the expression construct having at least one expression cassette, the expression cassette including an acid extracellular protease secretion signal sequence and flanking zeta sequence recombination sites.
This invention relates to method of detoxification and stabilization of implantable or transplantable biological material of human or animal origin, the method including the following steps: treatment of the material with an antibiotic solution; treatment of the material in a solution containing an organic acid surfactant bile acid; treatment of the material in a solution to remove the organic acid surfactant bile acid; and treatment of the material in a primary alcohol such as ethanol. The solution containing an organic acid surfactant bile acid contains a synergistic triple combination of the organic acid surfactant bile acid, an anionic surfactant, and a non-ionic surfactant.
The present invention relates to a treatment system having a barium carbonate (BaCO3) dispersed alkaline substrate (BDAS) for use in the remediation or at least partial remediation of mine drainage (MD) and/or environmental media contaminated with a source of MD. The invention utilizes chemical, biological and combined treatment systems remove high concentrations of sulfates, hardness, heavy metals and N-compounds, that may exist in the MD as well as high concentrations of alkalinity produced during the remediation process. The invention further extends to a process for treating MD and/or environmental media contaminated with MD and to an apparatus for use in this process.
B09C 1/02 - Extraction using liquids, e.g. washing, leaching
B09C 1/10 - Reclamation of contaminated soil microbiologically or by using enzymes
B09C 1/08 - Reclamation of contaminated soil chemically
A62D 3/02 - Processes for making harmful chemical substances harmless, or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
C02F 3/00 - Biological treatment of water, waste water, or sewage
C02F 1/00 - Treatment of water, waste water, or sewage
C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used
THIS invention relates to a mammographic tomography test object (hereinafter referred to as a phantom) and more particularly, but not exclusively, to a mammographic tomography test phantom for use in the verification of image reconstruction positions in digital breast tomosynthesis. The phantom includes a body made from an approximately tissue equivalent x-ray attenuating material; an upper spacer located on top of the body, and a lower spacer located below the body, the spacers being made from a substantially non- compressible material; and a plurality of x-ray opaque granules located at predetermined positions within the body.
Thermus scotoductus strain SA-01 that is responsible for the reduction of uranium (VI), in a source of uranium (VI), to uranium (IV), wherein the polypeptide comprises the amino acid sequence of SEQ ID No: 1.
The present invention relates to newly discovered, novel strains of thermophilic Geobacillus bacteria isolated from the Northam Platinum Mine (NPM) in South Africa. The present invention further relates to the isolation and characterization of novel thermostable enzymes responsible for the bioreduction of Pt (IV) to elemental Pt. The present invention also extends to the use of the isolated enzymes in the bioreduction of Pt (IV) to elemental Pt and in the microbial transformation of Pt (IV) to platinum nanoparticles. The invention further extends to a process for the bioremediation, or at least partial remediation, of a site contaminated with a source of Pt (IV).
A high voltage indicator (10) comprising an indicator head (16) in which is housed circuitry comprising a charging system and a detection system (22), wherein the charging system is embedded in a networked polymer (60) and the detection system (52) is embedded in a semiconducting composite (62) thereby to limit current leakages and allow for the proper distribution of an electrical field in the indicator head when brought in close proximity of a high voltage line (14), thus resulting in stable operation of the indicator.
The preparation of C-3 coupled biflavonoids and C-3 coupled biflavonoid analogs occurs from flavan-3-ones that are contacted with a compound having a nucleophilic aromatic moiety, in the presence of a Lewis acid where an intermediate compound is formed with a C-3 hydroxy group. A flavan-3-ol can be converted to a flavan-3-one as required. The intermediate compound is dehydrated to a flavene with a C-3-C-4 double bond. The flavene compound undergoes hydroboration-oxidation hydration to introduce a C-4 hydroxy group that can be oxidized to an oxo group or can be dihydroxylation to introduce hydroxy groups at the C-4 and C-3 carbons and dehydrated to a biflavonoid or biflavonoid analog having an oxo group at its C-4 carbon and substituted by the selected nucleophilic aromatic moiety on its C-3 carbon.
C07D 311/28 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
C07D 311/26 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
C07D 407/10 - Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing aromatic rings
15.
Isolation of a protein responsible for uranium (VI) reduction
Thermus scotoductus strain SA-01 that is responsible for the reduction of uranium (VI), in a source of uranium (VI), to uranium (IV), wherein the polypeptide comprises the amino acid sequence of SEQ ID No: 1.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
A method and apparatus are provided for conducting a microbiological process on a bulk material in which a quantity of the bulk material is loaded onto a waterproof lining forming part of a bio cell with a heat transfer arrangement below the quantity of bulk material or within its volume, or both. The moisture content of the bulk material is controlled by periodic or intermittent distribution of water into the bulk material in order to promote microbiological activity within the bulk material by means of microbes that may be either naturally occurring within the bulk material or may be selected and introduced into the bulk material according to a desired result. A leachate recovery installation collects leachate draining from the bulk material. The temperature within the bulk material is monitored and the temperature controlled in order to elevate or decrease the temperature thereof to approach a target temperature associated with enhanced microbial activity of microbes present within the bulk material. The apparatus includes a controller having an electronic micro-processor with the controller having inputs for association with a temperature detector and a moisture detector. The preferred apparatus includes solar powered thermal and electrical energy units.
This invention relates to B-ring aminoalkyl substituted chalcones and analogues thereof. It also relates to processes for the synthesis of such compounds and to the use of such compounds as medicaments, in particular but not exclusively for the treatment of cancer and malaria.
C07C 225/22 - Compounds containing amino groups and doubly-bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly-bound oxygen atoms not being part of a —CHO group, e.g. amino ketones having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton
18.
Method for the preparation of C-4 coupled flavonoids, proanthocyanidins and analogues thereof
The invention relates to a novel process for the preparation of C-4 coupled flavonoids, proanthocyanidins and analogues thereof. According to a specific application of the invention, there is provided a method for the preparation of proanthocyanidins and proanthocyanidin analogues.
C07D 311/62 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulfur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
This invention relates to a method of producing a fermented foodstuff including the steps of providing a primary growth medium; providing a microorganism starter culture in the pre-incubation phase; inoculating the primary growth medium with the starter culture; allowing the starter culture to proliferate to the linear growth phase to form an inoculant; providing a secondary bulk growth medium; inoculating the secondary bulk growth medium with the inoculant whilst in the linear growth phase; and allowing the inoculant to proliferate to ferment the secondary bulk medium into the fermented foodstuff. The applicant has found that the method according to the invention reduces the production time of yogurt with at least 20%.
A23C 9/127 - Fermented milk preparationsTreatment using microorganisms or enzymes using microorganisms of the genus lactobacteriaceae and other microorganisms or enzymes, e.g. kefir, koumiss
A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes
The present invention provides multi-phase dietary supplement products in which specific detrimental dietary supplement component combinations are absent and, in the preferred form of which, specific beneficial dietary supplement component combinations are present in multiple dosage formulations. The invention also provides for a method for supplementing the diet of a patient in need of diet supplementation comprising the steps of administering at least two dosage form formulations of the multi-stage dietary supplement product at spaced time intervals to the person.
The present invention relates to the isolation and characterization of a protein responsible for the reduction of uranium (Vl) to uranium (IV) The present invention extends to the use of the isolated protein in the reduction of uranium (Vl) to uranium (IV) and further extends to a process for the bioremediation, or at least partial remediation, of a site contaminated with a source of U (Vl) According to a first aspect thereof, the present invention provides an isolated polypeptide derived from Thermus scotoductus strain SA-01 that is responsible for the reduction of uranium (Vl), in a source of uranium (Vl), to uranium (IV)1 wherein the polypeptide comprises the amino acid sequence of SEQ ID No: 1.
The invention relates to methods for the preparation of an optically inactive and optically active compounds which are selected from the group consisting of C-3 coupled biflavonoids and C-3 coupled biflavonoid analogues from a starting material or intermediate which are respectively selected from the group consisting of optically inactive or optically active flavan-3-ols and optically active flavan-3-ones, the method comprising the steps of (a) providing an optically inactive or active compound having a fIavan-3-ol structure or a compound which is a flavan-3-one, (b) if a compound having a flavan-3-ol structure with a hydroxy group on the C-3 carbon is selected as starting material, converting the hydroxy group on the C-3 carbon of the compound having the flavan-3-ol structure to an oxo group to form a flavan-3-one of that compound, (c) providing a compound having a nucleophilic aromatic moiety, which compound is selected from the group of compounds having a nucleophilic aromatic moiety and which have flavonoid base structures and compounds having a nucleophilic aromatic moiety and which do not have a flavonoid base structure, (d) contacting the flavan-3-one provided by step (a) or obtained by step (b) with the compound containing the nucleophilic aromatic moiety in the presence of a Lewis acid; (e) forming a first intermediate compound wherein the oxo group on the C-3 carbon is converted to a hydroxy group by virtue of nucleophilic addition when the compound containing the nucleophilic aromatic moiety is contacted to the C-3 carbon of the flavan-3-one, (f) subjecting the first intermediate compound to dehydration so as to introduce a double bond between the C-3 carbon and C-4 carbon of the intermediate compound with the concomitant removal of the hydroxy group from the C-3 carbon to form an optically active flavene compound which is substituted by the nucleophilic aromatic moiety on the C-3 carbon, (g) optionally subjecting the resultant flavene compound to hydroboration-oxidation hydration thereby removing said double bond between the C-3 carbon and the C-4 carbon with the concomitant introduction of a hydroxy group at the C-4 carbon to form a second intermediate compound, (h) further optionally oxidizing the second intermediate compound of step (g) thereby converting the hydroxy group at the said C-4 carbon to an oxo group, thereby forming a biflavonoid or biflavonoid analogue which is substituted by the selected nucleophilic aromatic moiety on the C-3 carbon, (ι) further optionally, and alternatively to step (h), subjecting the resultant flavene compound of step (f) to OsC4 dihydroxylation thereby removing said double bond between the C-3 carbon and C-4 carbon with the concomitant introduction of a hydroxy group at the C-4 carbon and a hydroxy group at the C-3 carbon to form a third intermediate compound, and (j) subjecting the third intermediate compound to dehydration whereby the hydroxy group at the C-3 carbon is removed and a double bond is introduced between the C-3 carbon and C-4 carbon thereby forming an enol product and allowing such enol product to rearrange spontaneously to form a biflavonoid or biflavonoid analogue having an oxo group at its C-4 carbon and which is substituted by the selected nucleophilic aromatic moiety on its C-3 carbon.
C07D 311/28 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
23.
INHIBITORY ANTIBODY FRAGMENTS TO HUMAN TISSUE FACTOR
The present invention relates to a novel antibody fragment that inhibits the action of human Tissue Factor thus inhibiting blood coagulation The present antibody fragment may be obtainable by selection by means of phage display procedure or by way of recombinant techniques and can be used in a variety of methods for diagnosing, treating and/or preventing diseases or disorders
The invention relates to a novel process for the preparation of C-4 coupled flavonoids, proanthocyanidins and analogues thereof. According to a specific application of the invention, there is provided a method for the preparation of proanthocyanidins and proanthocyanidin analogues.
C07D 311/28 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
patents
