Abstract

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

IPC Classes  ?

• C12N 11/082 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C12P 21/06 - Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
• C12N 9/76 - TrypsinChymotrypsin

Abstract

Novel chromatographic materials for chromatographic separations, columns, kits, and methods for preparation and separations with a superficially porous material comprising a substantially nonporous core and one or more layers of a porous shell material surrounding the core. The material of the invention is comprised of superficially porous particles and a narrow particle size distribution. The material of the invention is comprised of a superficially porous monolith, the substantially nonporous core material is silica and the porous shell material is an inorganic/organic hybrid material.

IPC Classes  ?

• B01J 20/283 - Porous sorbents based on silica
• B01J 20/285 - Porous sorbents based on polymers
• B01J 20/32 - Impregnating or coating

Abstract

Disclosed herein are methods of mapping a sequence of an RNA molecule comprising the steps of hybridizing a protecting primer with a portion the RNA molecule to form RNA/primer hybrid, combining the RNA molecule, the primer, and a digestion assay, digesting the RNA molecule hybridized with the primer in the digestion assay, and analyzing the two or more RNA fragments using liquid chromatography-mass spectrometry to determine the sequence of nucleotides in the RNA molecule. Also disclosed herein are digestion assays for selectively cleaving an RNA molecule into two or more RNA fragments.

IPC Classes  ?

• C12Q 1/6872 - Methods for sequencing involving mass spectrometry
• C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6816 - Hybridisation assays characterised by the detection means

Abstract

A tray status indicator system comprises a tray comprising a plurality of wells. Each well in the plurality of wells is configured to receive a sample or reference material. A light emitting diode (LED) arrangement is below the plurality of wells. Each LED in the LED arrangement is below a well of the plurality of wells. The system also comprises an instrument control system that generates and outputs control signals to the LED arrangement to illuminate the each LED in the LED arrangement at a predetermined color of a plurality of different colors that indicate a state of a sample or reference in the well.

IPC Classes  ?

• G01N 1/36 - Embedding or analogous mounting of samples
• F21V 9/40 - Elements for modifying spectral properties, polarisation or intensity of the light emitted, e.g. filters with provision for controlling spectral properties, e.g. colour, or intensity
• F21Y 115/10 - Light-emitting diodes [LED]

Abstract

A tray status indicator system comprises a tray comprising a plurality of wells. Each well in the plurality of wells is configured to receive a sample or reference material. A light emitting diode (LED) arrangement is below the plurality of wells. Each LED in the LED arrangement is below a well of the plurality of wells. The system also comprises an instrument control system that generates and outputs control signals to the LED arrangement to illuminate the each LED in the LED arrangement at a predetermined color of a plurality of different colors that indicate a state of a sample or reference in the well.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

The present disclosure is directed to nonporous polymer particles having an average particle size of 1 to 10 microns and being functionalized with an enzyme, such as trypsin. The enzyme-immobilized particles, and immobilized enzyme reactors thereof, can be used in methods for on-line protein digestion.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C12N 9/76 - TrypsinChymotrypsin
• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12N 11/084 - Polymers containing vinyl alcohol units

Abstract

The present disclosure relates to chemically modified protease enzymes, Lys-C and Lys-N, that have enhanced autolysis resistance. Also disclosed herein are methods of using such protease enzymes for improving detection of target analyte proteins in an analytical assay.

IPC Classes  ?

• C12N 9/52 - Proteinases derived from bacteria
• C12Q 1/37 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase involving peptidase or proteinase

Abstract

The present disclosure relates to chemically modified protease enzymes, Lys-C and Lys- N, that have enhanced autolysis resistance. Also disclosed herein are methods of using such protease enzymes for improving detection of target analyte proteins in an analytical assay.

IPC Classes  ?

• C12N 9/52 - Proteinases derived from bacteria
• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides
• C12N 9/58 - Proteinases derived from fungi

Abstract

An apparatus for performing electrical and rheological measurements of a powder sample includes an upper geometry including a first electrode, a lower geometry including a bottom surface and a second electrode, a sidewall forming a perimeter located between the upper geometry and the bottom surface of the lower geometry, a sample gap located between the upper geometry and the lower geometry and within the perimeter of the sidewall, the powder sample locatable in the sample gap such that the first electrode and the second electrode are in contact with the powder sample, an electrical measurement system operably connected to each of the first electrode and the second electrode configured to measure electrical properties of the powder sample during a rheological test, and a mechanical measurement system configured to measure mechanical properties of the powder sample located in the sample gap during the rheological test.

IPC Classes  ?

• G01N 27/04 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane
• G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance

Abstract

A method of automated sample preparation for thermal analysis includes picking up, with an automated gripping system of an autosampler, a pan located in a pan well of a sample tray; and performing an automated sample preparation procedure including at least one of: providing the pan to a balance module by the automated gripping system, wherein the balance module is configured to perform weight measurement on the pan; and providing the pan to a crimping module by the automated gripping system after a sample is loaded into the pan, wherein the crimping module is configured to seal the pan with an automated crimping process.

IPC Classes  ?

• G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation

Abstract

An apparatus for performing electrical and rheological measurements of a powder sample includes an upper geometry including a first electrode, a lower geometry including a bottom surface and a second electrode, a sidewall forming a perimeter located between the upper geometry and the bottom surface of the lower geometry, a sample gap located between the upper geometry and the lower geometry and within the perimeter of the sidewall, the powder sample locatable in the sample gap such that the first electrode and the second electrode are in contact with the powder sample, an electrical measurement system operably connected to each of the first electrode and the second electrode configured to measure electrical properties of the powder sample during a rheological test, and a mechanical measurement system configured to measure mechanical properties of the powder sample located in the sample gap during the rheological test.

IPC Classes  ?

• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane

Abstract

A method of automated sample preparation for thermal analysis includes picking up, with an automated gripping system of an autosampler, a pan located in a pan well of a sample tray; and performing an automated sample preparation procedure including at least one of: providing the pan to a balance module by the automated gripping system, wherein the balance module is configured to perform weight measurement on the pan; and providing the pan to a crimping module by the automated gripping system after a sample is loaded into the pan, wherein the crimping module is configured to seal the pan with an automated crimping process.

IPC Classes  ?

• G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
• G01N 35/04 - Details of the conveyor system

Abstract

A sample tray for thermal analysis includes a tray body including a plurality of wells, wherein each well is configured to receive a sample pan and a sample lid. The sample pan is configured to receive a sample for thermal analysis, wherein the sample lid is configured to be crimped to the sample pan to create a seal between the sample lid and the sample pan, and the tray body is configured to be received into an automated sample preparation system to prepare samples for thermal analysis. A method of manufacturing a sample tray is also disclosed.

IPC Classes  ?

• B01L 9/06 - Test-tube standsTest-tube holders
• G01K 17/00 - Measuring quantity of heat
• G01N 5/04 - Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
• G01N 25/00 - Investigating or analysing materials by the use of thermal means

Abstract

A battery material containment device comprises a lid; a housing case that is constructed and arranged to hold a battery material during a thermal analysis operation, the housing case including a threaded region for receiving and removably coupling with the lid to form a gas-tight seal and securing the battery material in the housing case; at least one vent for providing a gas flow path for the release of gasses generated by the battery material in the housing case during the thermal analysis operation; and a heat flow pathway between the battery material and a sensor that is maintained during the thermal analysis operation.

IPC Classes  ?

• G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation

Abstract

A battery material containment device comprises a lid; a housing case that is constructed and arranged to hold a battery material during a thermal analysis operation, the housing case including a threaded region for receiving and removably coupling with the lid to form a gas-tight seal and securing the battery material in the housing case; at least one vent for providing a gas flow path for the release of gasses generated by the battery material in the housing case during the thermal analysis operation; and a heat flow pathway between the battery material and a sensor that is maintained during the thermal analysis operation.

IPC Classes  ?

• H01M 10/42 - Methods or arrangements for servicing or maintenance of secondary cells or secondary half-cells
• H01M 10/48 - Accumulators combined with arrangements for measuring, testing or indicating the condition of cells, e.g. the level or density of the electrolyte

Goods & Services

Chromatography columns for laboratory use; chromatography columns for use in a laboratory in connection with liquid chromatography and mass spectrometry instruments; liquid chromatography apparatus and mass spectrometry apparatus incorporating cartridges, vials, and plates; analytical equipment and instruments, namely, liquid chromatography and mass spectrometry apparatuses for laboratory use, for performing biological and chemical separations, purification, screening and identification for medical laboratory, clinical laboratory, and scientific laboratory use and replacement parts therefor; chromatography apparatus for laboratory use and replacement parts therefor; spectrometry apparatus for laboratory use and replacement parts therefor.

Goods & Services

Chemicals for liquid chromatography, namely chemical reagents, filler and columns. Chromatography columns; Chromatography columns for use in connection with liquid chromatography and mass spectrometry instruments.

Goods & Services

Robotic imaging apparatus for analyzing and characterizing particulates; Robotic imaging apparatus for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; Robotic imaging apparatus for particle analysis in protein, cell, and gene therapy formulations; Robotic imaging apparatus for quality control and stability testing of pharmaceutical and biopharmaceutical products; Robotic imaging apparatus for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; Laboratory imaging instruments for analyzing and characterizing particulates; Laboratory imaging instruments for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; Laboratory imaging instruments for particle analysis in protein, cell, and gene therapy formulations; Laboratory imaging instruments for quality control and stability testing of pharmaceutical and biopharmaceutical products; Laboratory imaging instruments for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; Downloadable computer operating software for laboratory imaging instruments and robotic imaging apparatus; none of the foregoing including a spectrometer.

Goods & Services

Robotic imaging apparatus for analyzing and characterizing particulates; robotic imaging apparatus for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; robotic imaging apparatus for particle analysis in protein, cell, and gene therapy formulations; robotic imaging apparatus for quality control and stability testing of pharmaceutical and biopharmaceutical products; robotic imaging apparatus for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; laboratory imaging instruments for analyzing and characterizing particulates; laboratory imaging instruments for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; laboratory imaging instruments for particle analysis in protein, cell, and gene therapy formulations; laboratory imaging instruments for quality control and stability testing of pharmaceutical and biopharmaceutical products; laboratory imaging instruments for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; downloadable computer operating software for laboratory imaging instruments and robotic imaging apparatus; downloadable computer software for processing digital images; downloadable computer software for analyzing particulates; downloadable computer software for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; downloadable computer software for particle analysis in protein, cell, and gene therapy formulations; downloadable computer software for quality control and stability testing of pharmaceutical and biopharmaceutical products; downloadable computer software for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability.

Goods & Services

Chromatography columns; Chromatography columns for use in connection with liquid chromatography and mass spectrometry instruments

Goods & Services

Robotic imaging apparatus for analyzing and characterizing particulates; Robotic imaging apparatus for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; Robotic imaging apparatus for particle analysis in protein, cell, and gene therapy formulations; Robotic imaging apparatus for quality control and stability testing of pharmaceutical and biopharmaceutical products; Robotic imaging apparatus for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; Laboratory imaging instruments for analyzing and characterizing particulates; Laboratory imaging instruments for analyzing cells, proteins, antibodies, DNA, RNA, viral vectors, capsids, lipid nanoparticles, exosomes, and therapeutic applications thereof; Laboratory imaging instruments for particle analysis in protein, cell, and gene therapy formulations; Laboratory imaging instruments for quality control and stability testing of pharmaceutical and biopharmaceutical products; Laboratory imaging instruments for detecting, measuring, and characterizing particulate contamination, viral vector aggregation, viral vector stability, viral vector formulations, excipient degradation, and cell viability; Downloadable computer operating software for laboratory imaging instruments and robotic imaging apparatus; none of the foregoing including a spectrometer

Goods & Services

Recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; recorded or downloadable software for laboratory analytical method development, testing, and validation management; recorded or downloadable software for managing laboratory workflow; recorded or downloadable software for purification of samples in chromatography and spectrometry applications; recorded or downloadable software for automating chromatography and mass spectrometry operations; recorded or downloadable software for laboratory and sample management; recorded or downloadable software for chromatography and spectrometry instruments; recorded or downloadable software for an application programming interface (API) for laboratory instruments; recorded or downloadable software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; recorded or downloadable software for quality assurance and quality control (QA/QC) of chemical compositions; recorded or downloadable software for controlling data integrity for compliance with government and industry regulation; recorded or downloadable software for environmental regulation compliance testing; recorded or downloadable software for managing compliance with government and industry regulations; recorded or downloadable software for electronic record keeping for quality assurance and quality control (QA/QC) testing; recorded or downloadable software for laboratory instrument management, reagent management, column management, and sample management; analytical equipment and instruments, not for medical use, for performing biological and chemical separation, purification, screening and identification, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor for laboratory use; chromatography instruments apparatus for laboratory use; chromatography instruments for use in connection with mass spectrometry instruments for laboratory use; chromatography instruments for use in connection with laboratory analytical software; chromatographic systems comprising of pumps, detectors, injectors, autosamplers, gradient controllers, mixing chambers, columns, recorders and fraction collectors for laboratory use; laboratory instruments for processing samples with liquid chromatography columns; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing. Software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; software as a service, or providing online software for laboratory analytical method development, testing, and validation management; software as a service, or providing online software for managing laboratory workflow; software as a service, or providing online software for automating chromatography and mass spectrometry operations; software as a service, or providing online software for laboratory and sample management; software as a service, or providing online software for chromatography and spectrometry instruments; software as a service, or providing online software for an application programming interface (API) for laboratory instruments; software as a service, or providing online software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; software as a service, or providing online software for quality assurance and quality control (QA/QC) of chemical compositions; software as a service, or providing online software for controlling data integrity for compliance with government and industry regulation; software as a service, or providing online software for environmental regulation compliance testing; software as a service, or providing online software for managing compliance with government and industry regulations; software as a service, or providing online software for electronic record keeping for quality assurance and quality control (QA/QC) testing; software as a service, or providing online software for laboratory instrument management, reagent management, column management, and sample management; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; providing temporary use of non-downloadable cloud-based software for collecting, manipulating, analyzing, and storing data for laboratory use, laboratory sample management, and for laboratory analytical method development, testing, and validation; consultation services relating to computer software; consultation about the maintenance and updating of computer software; providing technical advice relating to the operation of computer software for pharmaceutical regulation compliance, quality control, and testing; consulting services in the field of compliance with government and industry regulated laboratory testing requirements; consulting services in the field of quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; consulting services in the field of quality assurance and quality control (QA/QC) of chemical compositions; consulting services in the field of technical validation and verification of laboratory instruments; software as a service, or providing online software for purification of samples in chromatography and spectrometry applications.

Abstract

Disclosed is a liquid chromatography system and mixer for use therein that includes a first split connected to an inlet, the first split branching the flow of fluid from the inlet into a first path and a second path; a second split connected to an outlet of the first path, the second split branching the first path into a third path and a fourth path; and a third split connected to an outlet of the second path, the third split branching the second path into a fifth path and a sixth path. The first path and the second path are offset by a first predetermined volume, the third path and the fourth path are offset by a second predetermined volume, and the fifth path and the sixth path are also offset by the second predetermined volume.

IPC Classes  ?

• G01N 30/06 - Preparation
• G01N 30/02 - Column chromatography
• G01N 30/16 - Injection
• G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed

Abstract

Disclosed herein are methods of mapping a sequence of an RNA molecule comprising the steps of hybridizing a protecting primer with a portion the RNA molecule to form RNA/primer hybrid, combining the RNA molecule, the primer, and a digestion assay, digesting the RNA molecule hybridized with the primer in the digestion assay, and analyzing the two or more RNA fragments using liquid chromatography–mass spectrometry to determine the sequence of nucleotides in the RNA molecule. Also disclosed herein are digestion assays for selectively cleaving an RNA molecule into two or more RNA fragments.

IPC Classes  ?

• C12Q 1/6816 - Hybridisation assays characterised by the detection means

Goods & Services

Chemical test kits for indicating the presence or absence of toxins; chemical and biochemical reagents for non-medical purposes for indicating the presence or absence of toxins; chemically treated non-medical test strips for indicating the presence or absence of toxins; reagents for laboratory use; reagents for scientific and research use; analytical standard solutions and reagents for scientific and research use; analytical standard solutions and reagents for testing soil for the purpose of monitoring contaminants; chromatography chemicals; sample preparation kits for scientific and research purposes, comprising biological standards, namely, monoclonal antibodies, and chemical reagents for biological analysis; chemical reagents for preparing samples for chromatography and mass spectrometry for non-medical, laboratory use; chemicals, namely, buffers and standard solutions used in analytical chemistry; buffers in the nature of chemicals used in analytical chemistry; chemically-treated non-medical test strips and test-strip holders specifically adapted for, and sold with, the aforementioned test strips for the detection of mycotoxins by antibody affinity reactions in grain, feed, and food products; assay kits comprising an affinity chromatography column and chemical reagents for testing for mycotoxin contamination in grain, feed, and food products; environmental quality control fluid, gas, and soil samples for calculating standards for testing and verifying the quality of soil and air. Analytical apparatus and instruments for laboratory use and their parts, fittings, and accessories therefor; analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification; laboratory instruments, namely, chromatography and spectrometry instruments for laboratory use; material testing laboratory instruments; laboratory apparatus and instruments for solid phase extraction; positive pressure manifolds for chromatography instruments; laboratory analytical instruments for detecting and measuring visual and ultraviolet light; laboratory analytical instruments for detecting and measuring fluorescence; laboratory analytical instruments, namely, ultraviolet and visual light sensors; laboratory analytical instruments, namely, photodiode array sensors; laboratory analytical instruments, namely, evaporative light scattering detectors; laboratory analytical instruments, namely, refractive index detectors; analytical instruments featuring light-guiding flow cell for performing and analyzing biological and chemical samples, not for medical use; wheeled carts specially adapted for laboratory instruments; laboratory instruments for organizing, storing, and processing laboratory samples in a temperature controlled environment; fluid temperature control system for laboratory use and scientific purposes; laboratory instrument for controlling sample temperature; laboratory instrument for controlling sample temperature for use in connection with laboratory automation systems; laboratory instruments for processing samples with liquid chromatography columns; laboratory instruments for heating and cooling laboratory samples and chromatography columns; chromatography columns for laboratory use featuring radio frequency identification (RFID) transponders; radio frequency identification (RFID) transponders and readers for use with chromatography columns; radio frequency radio frequency identification (RFID) transponders, tags, labels, and stickers sold as components of a chromatography column; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing; spectral detectors for use in connection with mass spectrometers and liquid chromatographs not for medical use; detectors for detecting and quantifying ultraviolet and visible spectral exposure for laboratory use; florescence detectors for analysis and detection of samples containing chemiluminescent properties for laboratory use; laboratory robots; laboratory robots used for the purpose of handling laboratory pipettes; laboratory pipettes; automated laboratory pipettes; electronic laboratory pipettes; automated pipetting instrument for laboratory use for use in connection with liquid chromatography or mass spectrometry; pipette racks for laboratory use; pipette tips for laboratory use; pipette accessories, namely, magnetic beads used for laboratory use for DNA and RNA extraction within pipette tips; laboratory equipment and apparatus, namely, magnetic beads for laboratory use for use in purifying genomic, plasmid and mitochondrial DNA; accessories for analytical apparatus and instruments for scientific and laboratory research purposes, namely, peltier plates in the nature of electrical cooling and heating plates for cooling and heating laboratory samples; laboratory equipment, namely, orbital shakers for laboratory use; automated laboratory sample extraction instruments comprised of vacuum control valves and alignment posts; laboratory equipment and apparatus, namely, column tube grippers and microplate grippers for transfer of centrifuge tubes and microplates; laboratory equipment and supplies, namely, microfluidic chips for analyzing RNA and DNA; magnetic blocks for holding, organizing, agitating, stirring, heating, cooling, and storing consumables for laboratory use; laboratory equipment and supplies, namely, glass bottles, tanks, tubes, vials, and caps for tubes for laboratory use; laboratory equipment and supplies, namely, plastic tubes and bottles for laboratory use; plates for collecting and analyzing samples, namely, optimization plates, elution plates, photoredox plates, and well plates having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific or laboratory research use; non-medical immunostaining racks for scientific or laboratory purposes; disposable reusable dispenser syringes for laboratory sample use, not for medical use; laboratory instrument for measuring and locating the liquid levels of consumables for laboratory use in the nature of liquid level sensors; computer hardware for laboratory, scientific, and clinical use for use in managing laboratory operations and analyzing laboratory samples; electronic measurement systems, namely light scattering photometers, differential refractometers, online particle fractionators for use with light scattering photometers, sonication (non-chemical) optical flow cell cleaners; laboratory apparatus and instruments, namely multi-angel light scattering instruments; laboratory apparatus and instruments, namely dynamic light scattering instruments; laboratory apparatus and instruments, namely electrophoretic light scattering instruments; laboratory apparatus and instruments, namely field-flow fractionation instruments; laboratory analytical instruments for measuring and characterizing molecular molar mass, molecular size, molecular charge, molecular interaction, molecular shape and confirmation, and molecular conjugation and bonding; computer laboratory workstations comprised of downloadable and recorded software for scientific, clinical, and diagnostic laboratory use, namely, for use in managing laboratory operations, analyzing laboratory samples, and automating laboratory experiments; downloadable or recorded application programming interface (API) software; downloadable or recorded software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; downloadable or recorded software for analyzing samples for compliance with environmental regulations; downloadable or recorded software for analyzing samples for toxins or contaminants; downloadable or recorded software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; downloadable or recorded software for automating laboratory instruments; downloadable or recorded software for automating pipetting; downloadable or recorded software for calibrating pipettes; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; downloadable or recorded software for controlling chromatography and spectrometry instruments; downloadable or recorded software for controlling laboratory instruments; downloadable or recorded software for creating and executing laboratory methods and protocols; downloadable or recorded software for data analysis for laboratory instruments and laboratory data; downloadable or recorded software for facilitating laboratory compliance with government regulation; downloadable or recorded software for identifying and analyzing the components of a sample; downloadable or recorded software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; downloadable or recorded software for laboratory and laboratory sample management; downloadable or recorded software for laboratory informatics; downloadable or recorded software for laboratory robot control; downloadable or recorded software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; downloadable or recorded software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; downloadable or recorded software for operating laboratory instruments; downloadable or recorded software for purification of samples in chromatography and spectrometry applications; downloadable or recorded software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; downloadable or recorded software for validating pipette performance; downloadable or recorded software for validating processes in chromatography and mass spectrometry laboratories; downloadable or recorded software for validating processes in laboratories; analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification and structural parts and structural fittings therefor, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor, gas chromatography apparatus for laboratory use and structural parts and structural fittings therefor, ION mobility chromatography apparatus for laboratory use and structural parts and structural fittings therefor, mass spectrometry apparatus and instruments for laboratory use and structural parts and structural fittings therefor; analytical apparatus and instruments, namely, detectors for indicating the presence or absence of toxins in the fields of agriculture and food processing and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, magnetic suspension balances and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, mechanical testing instruments, rubber testing instruments and structural parts and structural fittings therefor; scientific analytical apparatus and instruments in the nature of magnetic suspension balances for isothermal measuring equilibrium and kinetics of ad-or absorption processes and for thermogravimetric measurements at high temperatures and high pressures, gravimetric analysis, thermogravimetric analysis or sorption analysis and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use for gas and vapor dosing, mixing, and pressure control and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, electrosurgical instruments used with analytical instruments to identify the presence or absence of toxins in sample tissues, electrosurgical smoke evaluation devices, devices for identification of chemicals in electrosurgical smoke for non-medical diagnostic and analytical purposes; fluorometers and structural parts and structural fittings therefor; lateral flow readers and structural parts and structural fittings therefor; rheometers and structural parts and structural fittings therefor; calorimeters and structural parts and structural fittings therefor; dilatometers and structural parts and structural fittings therefor; laboratory chemical reactors; diagnostic apparatus for testing food; diagnostic apparatus for testing agricultural products; diagnostic apparatus for the detection and identification of microbes and pathogens for laboratory or research use; diagnostic apparatus for testing for illegal drugs, diagnostic apparatus for forensic toxicology; chromatography columns for laboratory use; laboratory consumables, namely, vials, containers, sample trays, sample plates, sample containers, tubing, cartridges, pipettes; solid phase extraction cartridges and plates for laboratory use; affinity columns, comprising monoclonal antibodies, for purifying samples for subsequent liquid-chromatography and mass-spectrometry testing for mycotoxin contamination in grain, feed, and food products; computer operating software for analytical apparatus and instruments; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the materials; computer operating software for bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; analytical apparatus and instruments for laboratory use, namely, flash diffusivity analyzers and parts thereof; mechanical testing instruments, for testing the mechanical or material properties of test samples; mass spectrometer and parts thereof; analytical apparatus and instruments for analyzing mechanical or material properties of material samples for scientific research purposes, namely, thermal analyzers, thermal conductivity meters; laboratory instruments, namely, specimen testing instruments used to apply cyclic and static loads to a specimen integrated with a controller used for fatigue testing and material testing of the specimen subjected to loads; rheometers for measuring the viscosity and viscoelasticity of fluids, and parts and therefor; laboratory chemical reactors and parts thereof; laboratory instruments, namely, autosamplers; laboratory instruments, namely, pressure cells in the nature of vessels that can withstand high internal pressures; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; accessories for use with analytical apparatus and instruments for scientific research purposes, namely, grips and platens, stent and graft testers, cylinders, equipment for cutting specimens, and temperature control units; accessories for analytical apparatus and instruments for scientific research purposes, namely, peltier plate systems in the nature of electrical heating and cooling plates, plate pressure cells in the nature of vessels that can withstand high internal pressures, sensors for sensing the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression, electrically heated cylinders in the nature of test chambers, relative humidity sensors, optical plates, modular microscope accessory in the nature of microscopes, environmental test chambers, namely, temperature simulation equipment, small angle light scattering diodes, interfacial rheology accessories, namely, accessories for analyzing viscoelasticity, tribo-rheometry accessories, namely, accessories for analyzing friction, magneto-rheology accessories, namely, accessories for analyzing properties of materials subject to magnetic fields, electro-rheology accessories, namely, accessories for analyzing properties of materials subject to electric currents, UV curing accessories, namely, accessories for analyzing properties of materials subject to ultraviolet radiation, dieletric measurement accessories, namely, accessories for analyzing properties of materials subject to an oscillating electrical field, diaelectric thermal analysis accessories, namely, accessories for analyzing properties of materials subject to controlled temperatures and an oscillating electrical field, immobilization cells, namely, test chambers for characterizing the drying, retention, and immobilization kinetics of paints, coatings, and slurries; starch pasting cells, namely, test chambers for analyzing starches, dynamic mechanical analysis accessories, namely, accessories for analyzing material properties of solid materials, refrigerated cooling systems, namely, analytical instrument accessories for controlling sample temperature, laboratory instruments, namely, autosamplers and automated sample processing apparatus and carousels, forced convection oven accessories, namely, analytical instrument accessories for controlling sample temperature, orthogonal superposition accessories, namely, accessories for analyzing nonlinear viscoelasticity, 2-dimensional small amplitude oscillatory shear accessories, namely, accessories for analyzing nonlinear viscoelasticity, extensional viscosity fixture accessories, namely, accessories for analyzing extensional viscosity and stress, cone and partitioned plate accessories, namely, accessories for analyzing elastic materials, interfacial rheology accessories, degassing stations, namely, instruments for removing gasses from samples, clamps for use with analytical instruments, evacuated ampoules made of glass, metal, or plastic for use in testing and analyzing samples for non-medical purposes, sample measuring system accessories, namely, accessories for measuring the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression. Promoting the exchange of information and resources within the laboratory research community to achieve advances in the field of the separation sciences; promoting the exchange of information and resources within the laboratory research community to achieve advances in the fields of chemistry, biochemistry, pharmaceuticals, environmental science, material science, and material characterization; business consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, forensic toxicology, food science, environmental science, material science, and material characterization; online, catalog, and mail order services featuring computer operating software for analytical apparatus and instruments, analyzing and manipulating data; online, catalog, and mail order services featuring computer operating software data acquisition, processing, and reporting. Providing facilities for scientific research in the nature of a laboratory for the separation sciences; providing facilities for scientific research in the nature of a laboratory featuring instruments for chromatography, mass spectrometry, and material science; consulting services in the field of laboratory research for chemistry, biochemistry, pharmaceutical science, material science, and material characterization; consulting services in the field of laboratory science; technical consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; scientific research and development; laboratory study and research in the fields of chemistry, biochemistry, pharmaceutical science, material science, and material characterization; scientific laboratory research services in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; research and development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization; advisory services relating to the fields of development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization advisory services related to material testing; updating, repair, and maintenance of laboratory software; technical support services, namely, providing information, consultation and diagnostic services in the fields of electronic and optical components, computer software, and scientific, electronic test, measurement, biological and chemical analytical, and optical equipment; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; software as a service (SaaS) services featuring software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment software as a service (SaaS) services featuring software for analyzing samples for compliance with environmental regulations; software as a service (SaaS) services featuring software for analyzing samples for toxins or contaminants; software as a service (SaaS) services featuring software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; software as a service (SaaS) services featuring software for automating laboratory instruments; software as a service (SaaS) services featuring software for automating pipetting; software as a service (SaaS) services featuring software for calibrating pipettes; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; software as a service (SaaS) services featuring software for controlling chromatography and spectrometry instruments; software as a service (SaaS) services featuring software for controlling laboratory instruments; software as a service (SaaS) services featuring software for creating and executing laboratory methods and protocols; software as a service (SaaS) services featuring software for data analysis for laboratory instruments and laboratory data; software as a service (SaaS) services featuring software for facilitating laboratory compliance with government regulation; software as a service (SaaS) services featuring software for identifying and analyzing the components of a sample; software as a service (SaaS) services featuring software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; software as a service (SaaS) services featuring software for laboratory and laboratory sample management; software as a service (SaaS) services featuring software for laboratory informatics; software as a service (SaaS) services featuring software for laboratory robot control; software as a service (SaaS) services featuring software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; software as a service (SaaS) services featuring software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; software as a service (SaaS) services featuring software for operating laboratory instruments; software as a service (SaaS) services featuring software for purification of samples in chromatography and spectrometry applications; software as a service (SaaS) services featuring software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; software as a service (SaaS) services featuring software for validating pipette performance; software as a service (SaaS) services featuring software for validating processes in chromatography and mass spectrometry laboratories; software as a service (SaaS) services featuring software for validating processes in laboratories; providing online, non-downloadable software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; providing online, non-downloadable software for analyzing samples for compliance with environmental regulations; providing online, non-downloadable software for analyzing samples for toxins or contaminants; providing online, non-downloadable software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; providing online, non-downloadable software for automating laboratory instruments; providing online, non-downloadable software for automating pipetting; providing online, non-downloadable software for calibrating pipettes; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; providing online, non-downloadable software for controlling chromatography and spectrometry instruments; providing online, non-downloadable software for controlling laboratory instruments; providing online, non-downloadable software for creating and executing laboratory methods and protocols; providing online, non-downloadable software for data analysis for laboratory instruments and laboratory data; providing online, non-downloadable software for facilitating laboratory compliance with government regulation; providing online, non-downloadable software for identifying and analyzing the components of a sample; providing online, non-downloadable software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; providing online, non-downloadable software for laboratory and laboratory sample management; providing online, non-downloadable software for laboratory informatics; providing online, non-downloadable software for laboratory robot control; providing online, non-downloadable software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; providing online, non-downloadable software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; providing online, non-downloadable software for operating laboratory instruments; providing online, non-downloadable software for purification of samples in chromatography and spectrometry applications; providing online, non-downloadable software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; providing online, non-downloadable software for validating pipette performance; providing online, non-downloadable software for validating processes in chromatography and mass spectrometry laboratories; providing online, non-downloadable software for validating processes in laboratories; design and development of analytical apparatus and instruments and parts therefor for scientific and research use; design and development of chemistry consumables for laboratory use; design and development of computer software for analytical apparatus and instruments or scientific and research use; scientific consulting services in the field of analytical chemistry; scientific consulting services in the field of analytical chemistry in the nature of product qualification and validation, implementation project management of scientific research for chemical analyses, computer software testing for chemical analyses, laboratory analytics, and informatics for chemical analyses; scientific consulting in the field of analytical chemistry, namely, development of practical applications and usage of laboratory apparatus and instruments for use in research in analytical chemistry; cloud computing featuring software and software as a service featuring software for use with analytical apparatus and instruments in bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds, analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; maintenance and repair services for database systems and computer operating software.

Abstract

The present disclosure is directed to methods of performing affinity chromatography. The injection elution methods disclosed herein afford rapid and robust elution of a target analyte from an affinity chromatography column, therefore improving workflow efficiency. Further, the single injection elution methods result in highly concentrated samples, mitigating the need for sample concentration or manipulation.

IPC Classes  ?

• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups

Abstract

The present disclosure is directed to methods of performing direct affinity-size exclusion chromatography, wherein there is a direct elution of the sample from the affinity chromatography column to the SEC column. The methods described herein allow for rapid and robust purification of target analytes from heterogeneous samples, and mitigate the need for complicated valve switching, buffer exchange, or other sample manipulation.

IPC Classes  ?

• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns

Abstract

The present disclosure is directed to methods of performing direct affinity-size exclusion chromatography, wherein there is a direct elution of the sample from the affinity chromatography column to the SEC column. The methods described herein allow for rapid and robust purification of target analytes from heterogeneous samples, and mitigate the need for complicated valve switching, buffer exchange, or other sample manipulation.

IPC Classes  ?

• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• B01J 20/286 - Phases chemically bonded to a substrate, e.g. to silica or to polymers
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• G01N 30/02 - Column chromatography
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier

Abstract

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A device for removal of excess material from a test sample includes a test geometry comprising a lower geometry and an upper geometry, wherein at least one of the upper geometry and the lower geometry is configured to move vertically with respect to the other of the upper geometry and the lower geometry and a trimming device. The trimming device includes a first arm extending to a first set of blades including a first blade and a second blade, a second arm extending to a second set of blades including a third blade and a fourth blade, and linear track system. Movement of the trimming device along the linear track system first engages the first blade and the third blade with an exterior of at least one of the upper geometry and the lower geometry, and then engages the second blade and the fourth blade with the exterior of the at least one of the upper geometry and the lower geometry.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation

Abstract

Time sensitive biopharmaceutical processing updates can be implemented using lateral flow devices to test on-site during manufacturing. Lateral flow testing for an analyte such as, for example, impurities (e.g., host cell proteins), during manufacturing of a biopharmaceutical drug product is completed within twenty-five minutes of collecting a sample from the manufacturing line. That is, all sample preparation, such as adding reagent or diluting the sample, contacting the prepared sample to a lateral flow device, and detecting presence or absence of the analyte is completed within twenty-five minutes (e.g., 20 minutes, 18 minutes, 15 minutes) from collection such that processing adjustments during manufacturing can be implemented.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The present disclosure is directed to methods of performing affinity chromatography. The injection elution methods disclosed herein afford rapid and robust elution of a target analyte from an affinity chromatography column, therefore improving workflow efficiency. Further, the single injection elution methods result in highly concentrated samples, mitigating the need for sample concentration or manipulation.

IPC Classes  ?

• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01J 20/286 - Phases chemically bonded to a substrate, e.g. to silica or to polymers
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography

Abstract

A device for removal of excess material from a test sample includes a test geometry comprising a lower geometry and an upper geometry, wherein at least one of the upper geometry and the lower geometry is configured to move vertically with respect to the other of the upper geometry and the lower geometry and a trimming device. The trimming device includes a first arm extending to a first set of blades including a first blade and a second blade, a second arm extending to a second set of blades including a third blade and a fourth blade, and linear track system. Movement of the trimming device along the linear track system first engages the first blade and the third blade with an exterior of at least one of the upper geometry and the lower geometry, and then engages the second blade and the fourth blade with the exterior of the at least one of the upper geometry and the lower geometry.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation
• G01N 1/34 - PurifyingCleaning
• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane

Goods & Services

Kits comprising reagents not for medical use, chemically-treated test strips and holders for test strips for the detection of mycotoxins in grain, feed, and food products; Assay kits for detection of chemicals or toxins; Assay kits for testing for mycotoxin contamination in grain, feed, and food products; Assay kits for testing of chemicals or identifying toxins for laboratory use; Affinity columns, comprising monoclonal antibodies, for purifying samples for subsequent liquid-chromatography and mass-spectrometry testing for mycotoxin contamination in grain, feed, and food products; Chemical test kits for indicating the presence or absence of toxins for laboratory or research use; Test strips for indicating the presence or absence of toxins; Kits comprising monoclonal antibodies, buffers and reagents for use in the detection of chemicals or toxins for non-medical use; Prepacked affinity column and reagents for detecting chemicals and toxins; Chemical reagents Electronic detectors for indicating the presence or absence of toxins in the fields of agriculture and food processing; Measuring devices, namely, fluorometers; Lateral flow readers; Chromatography columns for laboratory use; Chromatography columns for detecting toxins not for medical use; Chromatography columns for preparing samples for subsequent chromatography and mass-spectrometry testing; Chromatography columns for subsequent chromatography and mass-spectrometry testing; Laboratory apparatus for detecting toxins and chemicals not for medical use; Electronic detectors for indicating the presence or absence of toxins in the fields of agriculture, animal feed, grains, foods and beverage, botanicals, and consumer products Providing scientific and laboratory information regarding toxin and chemical testing; Providing scientific and laboratory information regarding compliance with food, beverage, and agricultural testing regulations; Providing online non-downloadable software for facilitating compliance with government regulation; Providing access to an online database featuring laboratory information regarding toxin testing regulatory standards

Abstract

Exemplary embodiments eliminate the need for column ovens in serial column chromatography arrangements and systems by using insulated sleeves. The insulated sleeves may encase individual chromatography columns or clusters of chromatography columns. The use of the insulated sleeves allows the chromatography columns to be positioned in close proximity to each other. This may decrease the overall size of a serial column chromatography arrangement and may reduce costs by not requiring the column ovens.

IPC Classes  ?

• G01N 30/46 - Flow patterns using more than one column
• G01N 30/02 - Column chromatography
• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed

Abstract

The present disclosure is directed to methods for characterization of a sample by size exclusion chromatography (SEC), the sample including intact lipid nanoparticles (LNPs). The method generally includes loading the sample on a chromatographic column having an SEC packing material disposed therein, flowing a mobile phase through the SEC packing material to elute the intact LNPs, and detecting the eluted intact LNPs. The mobile phase includes an aqueous buffer and a branched poly-α-d-glucoside.

IPC Classes  ?

• G01N 30/16 - Injection
• G01N 30/02 - Column chromatography
• G01N 30/74 - Optical detectors

Abstract

The present disclosure pertains to compositions comprising a non-porous particle core coated with a hydrophilic polymer with surface-grafted polyionic chains. In some aspects, the present disclosure pertains to chromatographic separation devices that comprise such compositions. In some further aspects, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such compositions and (b) flowing a mobile phase through the column.

IPC Classes  ?

• B01J 41/14 - Macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
• B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
• B01J 41/05 - Processes using organic exchangers in the strongly basic form
• B01J 47/02 - Column or bed processes

Abstract

The present disclosure is directed to methods for characterization of a sample by size exclusion chromatography (SEC), the sample including intact lipid nanoparticles (LNPs). The method generally includes loading the sample on a chromatographic column having an SEC packing material disposed therein, flowing a mobile phase through the SEC packing material to elute the intact LNPs, and detecting the eluted intact LNPs. The mobile phase includes an aqueous buffer and a branched poly-α-d-glucoside.

IPC Classes  ?

• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• G01N 30/62 - Detectors specially adapted therefor

Abstract

Exemplary embodiments may provide a pneumatic transfer system for transferring fluid samples from a bioreactor to sample processing system without the need for manual transfer of the fluid samples. The exemplary embodiments may transfer fluid samples distances of at least 1 foot with the pneumatic transfer system without substantial loss of the fluid samples. Exemplary embodiments may recover at least 50% of the mass of the fluid samples at the destination. Exemplary embodiments may choose the pressure applied by one or more pumps so as to be sufficient enough so as to move the fluid samples at a reasonable rate without causing fragmentation of the fluid samples that results in non-negligible portions of the fluid samples sticking to the inner walls of the conduit in small droplets.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/26 - Inoculator or sampler

Abstract

Exemplary embodiments may provide a pneumatic transfer system for transferring fluid samples from a bioreactor to sample processing system without the need for manual transfer of the fluid samples. The exemplary embodiments may transfer fluid samples distances of at least 1 foot with the pneumatic transfer system without substantial loss of the fluid samples. Exemplary embodiments may recover at least 50% of the mass of the fluid samples at the destination. Exemplary embodiments may choose the pressure applied by one or more pumps so as to be sufficient enough so as to move the fluid samples at a reasonable rate without causing fragmentation of the fluid samples that results in non-negligible portions of the fluid samples sticking to the inner walls of the conduit in small droplets.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Goods & Services

Recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; recorded or downloadable software for laboratory analytical method development, testing, and validation management; recorded or downloadable software for managing laboratory workflow; recorded or downloadable software for purification of samples in chromatography and spectrometry applications; recorded or downloadable software for automating chromatography and mass spectrometry operations; recorded or downloadable software for laboratory and sample management; recorded or downloadable software for chromatography and spectrometry instruments; recorded or downloadable software for an application programming interface (API) for laboratory instruments; recorded or downloadable software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; recorded or downloadable software for quality assurance and quality control (QA/QC) of chemical compositions; recorded or downloadable software for controlling data integrity for compliance with government and industry regulation; recorded or downloadable software for environmental regulation compliance testing; recorded or downloadable software for managing compliance with government and industry regulations; recorded or downloadable software for electronic record keeping for quality assurance and quality control (QA/QC) testing; recorded or downloadable software for laboratory instrument management, reagent management, column management, and sample management; analytical equipment and instruments, not for medical use, for performing biological and chemical separation, purification, screening and identification, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor for laboratory use; chromatography instruments apparatus for laboratory use; chromatography instruments for use in connection with mass spectrometry instruments for laboratory use; chromatography instruments for use in connection with laboratory analytical software; chromatographic systems comprising of pumps, detectors, injectors, autosamplers, gradient controllers, mixing chambers, columns, recorders and fraction collectors for laboratory use; laboratory instruments for processing samples with liquid chromatography columns; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing. Software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; software as a service, or providing online software for laboratory analytical method development, testing, and validation management; software as a service, or providing online software for managing laboratory workflow; software as a service, or providing online software for automating chromatography and mass spectrometry operations; software as a service, or providing online software for laboratory and sample management; software as a service, or providing online software for chromatography and spectrometry instruments; software as a service, or providing online software for an application programming interface (API) for laboratory instruments; software as a service, or providing online software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; software as a service, or providing online software for quality assurance and quality control (QA/QC) of chemical compositions; software as a service, or providing online software for controlling data integrity for compliance with government and industry regulation; software as a service, or providing online software for environmental regulation compliance testing; software as a service, or providing online software for managing compliance with government and industry regulations; software as a service, or providing online software for electronic record keeping for quality assurance and quality control (QA/QC) testing; software as a service, or providing online software for laboratory instrument management, reagent management, column management, and sample management; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; providing temporary use of non-downloadable cloud-based software for collecting, manipulating, analyzing, and storing data for laboratory use, laboratory sample management, and for laboratory analytical method development, testing, and validation; consultation services relating to computer software; consultation about the maintenance and updating of computer software; providing technical advice relating to the operation of computer software for pharmaceutical regulation compliance, quality control, and testing; technical consulting services in the field of compliance with government and industry regulated laboratory testing requirements; consulting services in the field of quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; consulting services in the field of quality assurance and quality control (QA/QC) of chemical compositions; consulting services in the field of technical validation and verification of laboratory instruments; software as a service, or providing online software for purification of samples in chromatography and spectrometry applications.

Goods & Services

(1) Recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; recorded or downloadable software for laboratory analytical method development, testing, and validation management; recorded or downloadable software for managing laboratory workflow; recorded or downloadable software for purification of samples in chromatography and spectrometry applications; recorded or downloadable software for automating chromatography and mass spectrometry operations; recorded or downloadable software for laboratory and sample management; recorded or downloadable software for chromatography and spectrometry instruments; recorded or downloadable software for an application programming interface (API) for laboratory instruments; recorded or downloadable software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; recorded or downloadable software for quality assurance and quality control (QA/QC) of chemical compositions; recorded or downloadable software for controlling data integrity for compliance with government and industry regulation; recorded or downloadable software for environmental regulation compliance testing; recorded or downloadable software for managing compliance with government and industry regulations; recorded or downloadable software for electronic record keeping for quality assurance and quality control (QA/QC) testing; recorded or downloadable software for laboratory instrument management, reagent management, column management, and sample management; analytical equipment and instruments, not for medical use, for performing biological and chemical separation, purification, screening and identification, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor for laboratory use; chromatography instruments apparatus for laboratory use; chromatography instruments for use in connection with mass spectrometry instruments for laboratory use; chromatography instruments for use in connection with laboratory analytical software; chromatographic systems comprising of pumps, detectors, injectors, autosamplers, gradient controllers, mixing chambers, columns, recorders and fraction collectors for laboratory use; laboratory instruments for processing samples with liquid chromatography columns; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing. (1) Software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; software as a service, or providing online software for laboratory analytical method development, testing, and validation management; software as a service, or providing online software for managing laboratory workflow; software as a service, or providing online software for automating chromatography and mass spectrometry operations; software as a service, or providing online software for laboratory and sample management; software as a service, or providing online software for chromatography and spectrometry instruments; software as a service, or providing online software for an application programming interface (API) for laboratory instruments; software as a service, or providing online software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; software as a service, or providing online software for quality assurance and quality control (QA/QC) of chemical compositions; software as a service, or providing online software for controlling data integrity for compliance with government and industry regulation; software as a service, or providing online software for environmental regulation compliance testing; software as a service, or providing online software for managing compliance with government and industry regulations; software as a service, or providing online software for electronic record keeping for quality assurance and quality control (QA/QC) testing; software as a service, or providing online software for laboratory instrument management, reagent management, column management, and sample management; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; providing temporary use of non-downloadable cloud-based software for collecting, manipulating, analyzing, and storing data for laboratory use, laboratory sample management, and for laboratory analytical method development, testing, and validation; consultation services relating to computer software; consultation about the maintenance and updating of computer software; providing technical advice relating to the operation of computer software for pharmaceutical regulation compliance, quality control, and testing; consulting services in the field of compliance with government and industry regulated laboratory testing requirements; consulting services in the field of quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; consulting services in the field of quality assurance and quality control (QA/QC) of chemical compositions; consulting services in the field of technical validation and verification of laboratory instruments; software as a service, or providing online software for purification of samples in chromatography and spectrometry applications.

Abstract

Disclosed herein are methods for characterizing lipid nanoparticles (LNPs) using size-exclusion chromatography. The disclosed methods utilize a denaturing mobile phase (i.e., denaturing SEC) to afford robust disruption of LNPs and to permit the direct injection of LNPs onto a size-exclusion chromatography column. Accordingly, said methods allow for the characterization of formulated LNPs without pre-treatment or other modifications.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/74 - Optical detectors
• G01N 33/92 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving lipids, e.g. cholesterol

Abstract

The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a multilayer coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The multilayer coating is a vapor deposited and can include an outer layer formed of an inorganic-organic hybrid material.

IPC Classes  ?

• G01N 30/60 - Construction of the column
• C23C 16/02 - Pretreatment of the material to be coated
• C23C 16/04 - Coating on selected surface areas, e.g. using masks
• C23C 16/26 - Deposition of carbon only
• G01N 30/02 - Column chromatography

Abstract

i.ei.e., denaturing SEC) to afford robust disruption of LNPs and to permit the direct injection of LNPs onto a size-exclusion chromatography column. Accordingly, said methods allow for the characterization of formulated LNPs without pre-treatment or other modifications.

IPC Classes  ?

• A61K 9/1272 - Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers
• A61K 31/7088 - Compounds having three or more nucleosides or nucleotides
• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• C12N 15/88 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using liposome vesicle
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
• G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• B01D 15/12 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed

Goods & Services

Downloadable computer software.

Abstract

The present disclosure is directed to methods for characterization of a sample by size exclusion chromatography (SEC), the sample including intact lipid nanoparticles (LNPs). The method generally includes loading the sample on a chromatographic column having an SEC packing material disposed therein, flowing a mobile phase through the SEC packing material to elute the intact LNPs, and detecting the eluted intact LNPs. The mobile phase includes an organic solvent, an aqueous buffer, and a non-ionic surfactant.

IPC Classes  ?

• G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/56 - Packing methods or coating methods
• G01N 30/74 - Optical detectors

Abstract

The present disclosure is directed to methods for characterization of a sample by size exclusion chromatography (SEC), the sample including intact lipid nanoparticles (LNPs). The method generally includes loading the sample on a chromatographic column having an SEC packing material disposed therein, flowing a mobile phase through the SEC packing material to elute the intact LNPs, and detecting the eluted intact LNPs. The mobile phase includes an organic solvent, an aqueous buffer, and a non-ionic surfactant.

IPC Classes  ?

• A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
• G01N 30/74 - Optical detectors
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

Disclosed herein are methods for the separation of nucleic acids using slalom chromatography. The provided methods afford robust separation of nucleic acid species, including DNA, RNA, and mixtures thereof. Further, the methods are suitable for the separation of similar nucleic acid species that differ by topological conformations. Separation of nucleic acid species using the disclosed chromatographic methods can be achieved in under 10 minutes (e.g., between 1 and 4 minutes) while maintaining high levels of efficiency and peak resolution.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A thermal enclosure for a differential scanning calorimeter (DSC) instrument comprises a plurality of first thermal shields, each first thermal shield constructed and arranged for positioning about a DSC unit of a plurality of DSC units coupled to a temperature control plate; and a second thermal shield constructed and arranged for positioning about the plurality of DSC units and the plurality of first thermal shields.

IPC Classes  ?

• G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
• G01K 17/00 - Measuring quantity of heat

Abstract

Disclosed herein are methods for the separation of nucleic acids using slalom chromatography. The provided methods afford robust separation of nucleic acid species, including DNA, RNA, and mixtures thereof. Further, the methods are suitable for the separation of similar nucleic acid species that differ by topological conformations. Separation of nucleic acid species using the disclosed chromatographic methods can be achieved in under 10 minutes (e.g., between 1 and 4 minutes) while maintaining high levels of efficiency and peak resolution.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed
• G01N 30/74 - Optical detectors
• G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups

Abstract

A thermal enclosure for a differential scanning calorimeter (DSC) instrument comprises a plurality of first thermal shields, each first thermal shield constructed and arranged for positioning about a DSC unit of a plurality of DSC units coupled to a temperature control plate; and a second thermal shield constructed and arranged for positioning about the plurality of DSC units and the plurality of first thermal shields.

IPC Classes  ?

• G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
• G01K 17/00 - Measuring quantity of heat

Abstract

A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 μM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.

IPC Classes  ?

• C12N 15/115 - Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith
• C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
• G01N 33/561 - Immunoelectrophoresis
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The present technology provides a description of a system and method for purifying glycans. The system includes a polypeptide capture device fluidically coupled to a glycan capture and labeling device. The polypeptide device includes an affinity capture agent that can capture glycoproteins. The glycan capture and labeling device includes glycan affinity capture agents (e.g., lectin proteins) that can capture specific glycans released from glycoproteins in the polypeptide capture device. The captured glycans are subsequently tagged with a dye. The tagged glycans are removed from the lectins and passed into an analytical device.

IPC Classes  ?

• C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography

Abstract

The present technology provides a description of a system and method for purifying glycans. The system includes a polypeptide capture device fluidically coupled to a glycan capture and labeling device. The polypeptide device includes an affinity capture agent that can capture glycoproteins. The glycan capture and labeling device includes glycan affinity capture agents (e.g., lectin proteins) that can capture specific glycans released from glycoproteins in the polypeptide capture device. The captured glycans are subsequently tagged with a dye. The tagged glycans are removed from the lectins and passed into an analytical device.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/577 - ImmunoassayBiospecific binding assayMaterials therefor involving monoclonal antibodies

Abstract

The present invention provides novel methods for the chromatographic analysis of glycans using high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier and a labeling reagent which is capable of providing amphipathic and strongly base labeling moieties to a sample to be analyzed.

IPC Classes  ?

• C08B 37/00 - Preparation of polysaccharides not provided for in groups Derivatives thereof
• B01D 15/32 - Bonded phase chromatography, e.g. with normal bonded phase, reversed phase or hydrophobic interaction
• B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Goods & Services

(1) Chemical test kits for indicating the presence or absence of toxins; chemical and biochemical reagents for non-medical purposes for indicating the presence or absence of toxins; chemically treated non-medical test strips for indicating the presence or absence of toxins; reagents for laboratory use; reagents for scientific and research use; analytical standard solutions and reagents for scientific and research use; analytical standard solutions and reagents for testing soil for the purpose of monitoring contaminants; chromatography chemicals; sample preparation kits for scientific and research purposes, comprising biological standards, namely, monoclonal antibodies, and chemical reagents for biological analysis; chemical reagents for preparing samples for chromatography and mass spectrometry for non-medical, laboratory use; chemicals, namely, buffers and standard solutions used in analytical chemistry; buffers in the nature of chemicals used in analytical chemistry; chemically-treated non-medical test strips and test-strip holders specifically adapted for, and sold with, the aforementioned test strips for the detection of mycotoxins by antibody affinity reactions in grain, feed, and food products; assay kits comprising an affinity chromatography column and chemical reagents for testing for mycotoxin contamination in grain, feed, and food products; environmental quality control fluid, gas, and soil samples for calculating standards for testing and verifying the quality of soil and air. (2) Analytical apparatus and instruments for laboratory use and their parts, fittings, and accessories therefor; analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification; laboratory instruments, namely, chromatography and spectrometry instruments for laboratory use; material testing laboratory instruments; laboratory apparatus and instruments for solid phase extraction; positive pressure manifolds for chromatography instruments; laboratory analytical instruments for detecting and measuring visual and ultraviolet light; laboratory analytical instruments for detecting and measuring fluorescence; laboratory analytical instruments, namely, ultraviolet and visual light sensors; laboratory analytical instruments, namely, photodiode array sensors; laboratory analytical instruments, namely, evaporative light scattering detectors; laboratory analytical instruments, namely, refractive index detectors; analytical instruments featuring light-guiding flow cell for performing and analyzing biological and chemical samples, not for medical use; wheeled carts specially adapted for laboratory instruments; laboratory instruments for organizing, storing, and processing laboratory samples in a temperature controlled environment; fluid temperature control system for laboratory use and scientific purposes; laboratory instrument for controlling sample temperature; laboratory instrument for controlling sample temperature for use in connection with laboratory automation systems; laboratory instruments for processing samples with liquid chromatography columns; laboratory instruments for heating and cooling laboratory samples and chromatography columns; chromatography columns for laboratory use featuring radio frequency identification (RFID) transponders; radio frequency identification (RFID) transponders and readers for use with chromatography columns; radio frequency radio frequency identification (RFID) transponders, tags, labels, and stickers sold as components of a chromatography column; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing; spectral detectors for use in connection with mass spectrometers and liquid chromatographs not for medical use; detectors for detecting and quantifying ultraviolet and visible spectral exposure for laboratory use; florescence detectors for analysis and detection of samples containing chemiluminescent properties for laboratory use; laboratory robots; laboratory robots used for the purpose of handling laboratory pipettes; laboratory pipettes; automated laboratory pipettes; electronic laboratory pipettes; automated pipetting instrument for laboratory use for use in connection with liquid chromatography or mass spectrometry; pipette racks for laboratory use; pipette tips for laboratory use; pipette accessories, namely, magnetic beads used for laboratory use for DNA and RNA extraction within pipette tips; laboratory equipment and apparatus, namely, magnetic beads for laboratory use for use in purifying genomic, plasmid and mitochondrial DNA; accessories for analytical apparatus and instruments for scientific and laboratory research purposes, namely, peltier plates in the nature of electrical cooling and heating plates for cooling and heating laboratory samples; laboratory equipment, namely, orbital shakers for laboratory use; automated laboratory sample extraction instruments comprised of vacuum control valves and alignment posts; laboratory equipment and apparatus, namely, column tube grippers and microplate grippers for transfer of centrifuge tubes and microplates; laboratory equipment and supplies, namely, microfluidic chips for analyzing RNA and DNA; magnetic blocks for holding, organizing, agitating, stirring, heating, cooling, and storing consumables for laboratory use; laboratory equipment and supplies, namely, glass bottles, tanks, tubes, vials, and caps for tubes for laboratory use; laboratory equipment and supplies, namely, plastic tubes and bottles for laboratory use; plates for collecting and analyzing samples, namely, optimization plates, elution plates, photoredox plates, and well plates having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific or laboratory research use; non-medical immunostaining racks for scientific or laboratory purposes; disposable reusable dispenser syringes for laboratory sample use, not for medical use; laboratory instrument for measuring and locating the liquid levels of consumables for laboratory use in the nature of liquid level sensors; computer hardware for laboratory, scientific, and clinical use for use in managing laboratory operations and analyzing laboratory samples; electronic measurement systems, namely light scattering photometers, differential refractometers, online particle fractionators for use with light scattering photometers, sonication (non-chemical) optical flow cell cleaners; laboratory apparatus and instruments, namely multi-angel light scattering instruments; laboratory apparatus and instruments, namely dynamic light scattering instruments; laboratory apparatus and instruments, namely electrophoretic light scattering instruments; laboratory apparatus and instruments, namely field-flow fractionation instruments; laboratory analytical instruments for measuring and characterizing molecular molar mass, molecular size, molecular charge, molecular interaction, molecular shape and confirmation, and molecular conjugation and bonding; computer laboratory workstations comprised of downloadable and recorded software for scientific, clinical, and diagnostic laboratory use, namely, for use in managing laboratory operations, analyzing laboratory samples, and automating laboratory experiments; downloadable or recorded application programming interface (API) software; downloadable or recorded software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; downloadable or recorded software for analyzing samples for compliance with environmental regulations; downloadable or recorded software for analyzing samples for toxins or contaminants; downloadable or recorded software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; downloadable or recorded software for automating laboratory instruments; downloadable or recorded software for automating pipetting; downloadable or recorded software for calibrating pipettes; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; downloadable or recorded software for controlling chromatography and spectrometry instruments; downloadable or recorded software for controlling laboratory instruments; downloadable or recorded software for creating and executing laboratory methods and protocols; downloadable or recorded software for data analysis for laboratory instruments and laboratory data; downloadable or recorded software for facilitating laboratory compliance with government regulation; downloadable or recorded software for identifying and analyzing the components of a sample; downloadable or recorded software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; downloadable or recorded software for laboratory and laboratory sample management; downloadable or recorded software for laboratory informatics; downloadable or recorded software for laboratory robot control; downloadable or recorded software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; downloadable or recorded software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; downloadable or recorded software for operating laboratory instruments; downloadable or recorded software for purification of samples in chromatography and spectrometry applications; downloadable or recorded software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; downloadable or recorded software for validating pipette performance; downloadable or recorded software for validating processes in chromatography and mass spectrometry laboratories; downloadable or recorded software for validating processes in laboratories; analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification and structural parts and structural fittings therefor, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor, gas chromatography apparatus for laboratory use and structural parts and structural fittings therefor, ION mobility chromatography apparatus for laboratory use and structural parts and structural fittings therefor, mass spectrometry apparatus and instruments for laboratory use and structural parts and structural fittings therefor; analytical apparatus and instruments, namely, detectors for indicating the presence or absence of toxins in the fields of agriculture and food processing and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, magnetic suspension balances and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, mechanical testing instruments, rubber testing instruments and structural parts and structural fittings therefor; scientific analytical apparatus and instruments in the nature of magnetic suspension balances for isothermal measuring equilibrium and kinetics of ad-or absorption processes and for thermogravimetric measurements at high temperatures and high pressures, gravimetric analysis, thermogravimetric analysis or sorption analysis and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use for gas and vapor dosing, mixing, and pressure control and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, electrosurgical instruments used with analytical instruments to identify the presence or absence of toxins in sample tissues, electrosurgical smoke evaluation devices, devices for identification of chemicals in electrosurgical smoke for non-medical diagnostic and analytical purposes; fluorometers and structural parts and structural fittings therefor; lateral flow readers and structural parts and structural fittings therefor; rheometers and structural parts and structural fittings therefor; calorimeters and structural parts and structural fittings therefor; dilatometers and structural parts and structural fittings therefor; laboratory chemical reactors; diagnostic apparatus for testing food; diagnostic apparatus for testing agricultural products; diagnostic apparatus for the detection and identification of microbes and pathogens for laboratory or research use; diagnostic apparatus for testing for illegal drugs, diagnostic apparatus for forensic toxicology; chromatography columns for laboratory use; laboratory consumables, namely, vials, containers, sample trays, sample plates, sample containers, tubing, cartridges, pipettes; solid phase extraction cartridges and plates for laboratory use; affinity columns, comprising monoclonal antibodies, for purifying samples for subsequent liquid-chromatography and mass-spectrometry testing for mycotoxin contamination in grain, feed, and food products; computer operating software for analytical apparatus and instruments; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the materials; computer operating software for bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; analytical apparatus and instruments for laboratory use, namely, flash diffusivity analyzers and parts thereof; mechanical testing instruments, for testing the mechanical or material properties of test samples; mass spectrometer and parts thereof; analytical apparatus and instruments for analyzing mechanical or material properties of material samples for scientific research purposes, namely, thermal analyzers, thermal conductivity meters; laboratory instruments, namely, specimen testing instruments used to apply cyclic and static loads to a specimen integrated with a controller used for fatigue testing and material testing of the specimen subjected to loads; rheometers for measuring the viscosity and viscoelasticity of fluids, and parts and therefor; laboratory chemical reactors and parts thereof; laboratory instruments, namely, autosamplers; laboratory instruments, namely, pressure cells in the nature of vessels that can withstand high internal pressures; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; accessories for use with analytical apparatus and instruments for scientific research purposes, namely, grips and platens, stent and graft testers, cylinders, equipment for cutting specimens, and temperature control units; accessories for analytical apparatus and instruments for scientific research purposes, namely, peltier plate systems in the nature of electrical heating and cooling plates, plate pressure cells in the nature of vessels that can withstand high internal pressures, sensors for sensing the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression, electrically heated cylinders in the nature of test chambers, relative humidity sensors, optical plates, modular microscope accessory in the nature of microscopes, environmental test chambers, namely, temperature simulation equipment, small angle light scattering diodes, interfacial rheology accessories, namely, accessories for analyzing viscoelasticity, tribo-rheometry accessories, namely, accessories for analyzing friction, magneto-rheology accessories, namely, accessories for analyzing properties of materials subject to magnetic fields, electro-rheology accessories, namely, accessories for analyzing properties of materials subject to electric currents, UV curing accessories, namely, accessories for analyzing properties of materials subject to ultraviolet radiation, dieletric measurement accessories, namely, accessories for analyzing properties of materials subject to an oscillating electrical field, diaelectric thermal analysis accessories, namely, accessories for analyzing properties of materials subject to controlled temperatures and an oscillating electrical field, immobilization cells, namely, test chambers for characterizing the drying, retention, and immobilization kinetics of paints, coatings, and slurries; starch pasting cells, namely, test chambers for analyzing starches, dynamic mechanical analysis accessories, namely, accessories for analyzing material properties of solid materials, refrigerated cooling systems, namely, analytical instrument accessories for controlling sample temperature, laboratory instruments, namely, autosamplers and automated sample processing apparatus and carousels, forced convection oven accessories, namely, analytical instrument accessories for controlling sample temperature, orthogonal superposition accessories, namely, accessories for analyzing nonlinear viscoelasticity, 2-dimensional small amplitude oscillatory shear accessories, namely, accessories for analyzing nonlinear viscoelasticity, extensional viscosity fixture accessories, namely, accessories for analyzing extensional viscosity and stress, cone and partitioned plate accessories, namely, accessories for analyzing elastic materials, interfacial rheology accessories, degassing stations, namely, instruments for removing gasses from samples, clamps for use with analytical instruments, evacuated ampoules made of glass, metal, or plastic for use in testing and analyzing samples for non-medical purposes, sample measuring system accessories, namely, accessories for measuring the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression. (1) Promoting the exchange of information and resources within the laboratory research community to achieve advances in the field of the separation sciences; promoting the exchange of information and resources within the laboratory research community to achieve advances in the fields of chemistry, biochemistry, pharmaceuticals, environmental science, material science, and material characterization; business consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, forensic toxicology, food science, environmental science, material science, and material characterization; online, catalog, and mail order services featuring computer operating software for analytical apparatus and instruments, analyzing and manipulating data; online, catalog, and mail order services featuring computer operating software data acquisition, processing, and reporting. (2) Providing facilities for scientific research in the nature of a laboratory for the separation sciences; providing facilities for scientific research in the nature of a laboratory featuring instruments for chromatography, mass spectrometry, and material science; consulting services in the field of laboratory research for chemistry, biochemistry, pharmaceutical science, material science, and material characterization; consulting services in the field of laboratory science; technical consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; scientific research and development; laboratory study and research in the fields of chemistry, biochemistry, pharmaceutical science, material science, and material characterization; scientific laboratory research services in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; research and development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization; advisory services relating to the fields of development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization advisory services related to material testing; updating, repair, and maintenance of laboratory software; technical support services, namely, providing information, consultation and diagnostic services in the fields of electronic and optical components, computer software, and scientific, electronic test, measurement, biological and chemical analytical, and optical equipment; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; software as a service (SaaS) services featuring software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment software as a service (SaaS) services featuring software for analyzing samples for compliance with environmental regulations; software as a service (SaaS) services featuring software for analyzing samples for toxins or contaminants; software as a service (SaaS) services featuring software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; software as a service (SaaS) services featuring software for automating laboratory instruments; software as a service (SaaS) services featuring software for automating pipetting; software as a service (SaaS) services featuring software for calibrating pipettes; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; software as a service (SaaS) services featuring software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; software as a service (SaaS) services featuring software for controlling chromatography and spectrometry instruments; software as a service (SaaS) services featuring software for controlling laboratory instruments; software as a service (SaaS) services featuring software for creating and executing laboratory methods and protocols; software as a service (SaaS) services featuring software for data analysis for laboratory instruments and laboratory data; software as a service (SaaS) services featuring software for facilitating laboratory compliance with government regulation; software as a service (SaaS) services featuring software for identifying and analyzing the components of a sample; software as a service (SaaS) services featuring software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; software as a service (SaaS) services featuring software for laboratory and laboratory sample management; software as a service (SaaS) services featuring software for laboratory informatics; software as a service (SaaS) services featuring software for laboratory robot control; software as a service (SaaS) services featuring software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; software as a service (SaaS) services featuring software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; software as a service (SaaS) services featuring software for operating laboratory instruments; software as a service (SaaS) services featuring software for purification of samples in chromatography and spectrometry applications; software as a service (SaaS) services featuring software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; software as a service (SaaS) services featuring software for validating pipette performance; software as a service (SaaS) services featuring software for validating processes in chromatography and mass spectrometry laboratories; software as a service (SaaS) services featuring software for validating processes in laboratories; providing online, non-downloadable software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; providing online, non-downloadable software for analyzing samples for compliance with environmental regulations; providing online, non-downloadable software for analyzing samples for toxins or contaminants; providing online, non-downloadable software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; providing online, non-downloadable software for automating laboratory instruments; providing online, non-downloadable software for automating pipetting; providing online, non-downloadable software for calibrating pipettes; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; providing online, non-downloadable software for controlling chromatography and spectrometry instruments; providing online, non-downloadable software for controlling laboratory instruments; providing online, non-downloadable software for creating and executing laboratory methods and protocols; providing online, non-downloadable software for data analysis for laboratory instruments and laboratory data; providing online, non-downloadable software for facilitating laboratory compliance with government regulation; providing online, non-downloadable software for identifying and analyzing the components of a sample; providing online, non-downloadable software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; providing online, non-downloadable software for laboratory and laboratory sample management; providing online, non-downloadable software for laboratory informatics; providing online, non-downloadable software for laboratory robot control; providing online, non-downloadable software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; providing online, non-downloadable software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; providing online, non-downloadable software for operating laboratory instruments; providing online, non-downloadable software for purification of samples in chromatography and spectrometry applications; providing online, non-downloadable software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; providing online, non-downloadable software for validating pipette performance; providing online, non-downloadable software for validating processes in chromatography and mass spectrometry laboratories; providing online, non-downloadable software for validating processes in laboratories; design and development of analytical apparatus and instruments and parts therefor for scientific and research use; design and development of chemistry consumables for laboratory use; design and development of computer software for analytical apparatus and instruments or scientific and research use; scientific consulting services in the field of analytical chemistry; scientific consulting services in the field of analytical chemistry in the nature of product qualification and validation, implementation project management of scientific research for chemical analyses, computer software testing for chemical analyses, laboratory analytics, and informatics for chemical analyses; scientific consulting in the field of analytical chemistry, namely, development of practical applications and usage of laboratory apparatus and instruments for use in research in analytical chemistry; cloud computing featuring software and software as a service featuring software for use with analytical apparatus and instruments in bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds, analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in ITS environment; maintenance and repair services for database systems and computer operating software.

Goods & Services

Recorded or downloadable software for collecting, analyzing, storing, and reporting chromatography instrument utilization data; recorded or downloadable software for laboratory asset management, laboratory resource management, laboratory workflow management and analysis, analysis of utilization of laboratory instruments; recorded or downloadable software for validating laboratory processes.

Abstract

The exemplary embodiments provide chromatography column positioning assemblies that can ensure that the distance between face seals or other sealing surfaces/mechanisms at the respective ends of a liquid chromatography column is a desired distance (i.e., the length of the liquid chromatography column). The exemplary embodiments can adjust the separation between the face seals to accommodate different length liquid chromatography columns. For example, a chromatography column positioning assembly of an exemplary embodiment can set the distance between face seals to accommodate a 25 mm column, a 50 mm column or a 100 mm column.

IPC Classes  ?

• G01N 30/60 - Construction of the column
• B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
• G01N 30/46 - Flow patterns using more than one column

Abstract

The present disclosure pertains to compositions comprising a non-porous particle core coated with a hydrophilic polymer with surface-grafted polyionic chains. In some aspects, the present disclosure pertains to chromatographic separation devices that comprise such compositions. In some further aspects, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such compositions and (b) flowing a mobile phase through the column.

IPC Classes  ?

• B01J 41/05 - Processes using organic exchangers in the strongly basic form
• B01J 41/07 - Processes using organic exchangers in the weakly basic form
• B01J 41/20 - Anion exchangers for chromatographic processes

Goods & Services

Recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; recorded or downloadable software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; recorded or downloadable software for laboratory analytical method development, testing, and validation management; recorded or downloadable software for managing laboratory workflow; recorded or downloadable software for purification of samples in chromatography and spectrometry applications; recorded or downloadable software for automating chromatography and mass spectrometry operations; recorded or downloadable software for laboratory and sample management; recorded or downloadable software for chromatography and spectrometry instruments; recorded or downloadable software for an application programming interface (API) for laboratory instruments; recorded or downloadable software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; recorded or downloadable software for quality assurance and quality control (QA/QC) of chemical compositions; recorded or downloadable software for controlling data integrity for compliance with government and industry regulation; recorded or downloadable software for environmental regulation compliance testing; recorded or downloadable software for managing compliance with government and industry regulations; recorded or downloadable software for electronic record keeping for quality assurance and quality control (QA/QC) testing; recorded or downloadable software for laboratory instrument management, reagent management, column management, and sample management; analytical equipment and instruments, not for medical use, for performing biological and chemical separation, purification, screening and identification, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor for laboratory use; chromatography instruments apparatus for laboratory use; chromatography instruments for use in connection with mass spectrometry instruments for laboratory use; chromatography instruments for use in connection with laboratory analytical software; chromatographic systems comprising of pumps, detectors, injectors, autosamplers, gradient controllers, mixing chambers, columns, recorders and fraction collectors for laboratory use; laboratory instruments for processing samples with liquid chromatography columns; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing Software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data in chromatography and mass spectrometry applications; software as a service, or providing online software for collecting, manipulating, analyzing, storing, and reporting data for laboratory use; software as a service, or providing online software for laboratory analytical method development, testing, and validation management; software as a service, or providing online software for managing laboratory workflow; software as a service, or providing online software for automating chromatography and mass spectrometry operations; software as a service, or providing online software for laboratory and sample management; software as a service, or providing online software for chromatography and spectrometry instruments; software as a service, or providing online software for an application programming interface (API) for laboratory instruments; software as a service, or providing online software for quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; software as a service, or providing online software for quality assurance and quality control (QA/QC) of chemical compositions; software as a service, or providing online software for controlling data integrity for compliance with government and industry regulation; software as a service, or providing online software for environmental regulation compliance testing; software as a service, or providing online software for managing compliance with government and industry regulations; software as a service, or providing online software for electronic record keeping for quality assurance and quality control (QA/QC) testing; software as a service, or providing online software for laboratory instrument management, reagent management, column management, and sample management; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; providing temporary use of non-downloadable cloud-based software for collecting, manipulating, analyzing, and storing data for laboratory use, laboratory sample management, and for laboratory analytical method development, testing, and validation; consultation services relating to computer software; consultation about the maintenance and updating of computer software; providing technical advice relating to the operation of computer software for pharmaceutical regulation compliance, quality control, and testing; consulting services in the field of compliance with government and industry regulated laboratory testing requirements; consulting services in the field of quality assurance and quality control (QA/QC) of pharmaceuticals, biopharmaceuticals, drugs, medicines, supplements, nutraceuticals, foods, beverages, clinical diagnostic products, cosmetics, personal care products, electronics, and medical devices; consulting services in the field of quality assurance and quality control (QA/QC) of chemical compositions; consulting services in the field of technical validation and verification of laboratory instruments; software as a service, or providing online software for purification of samples in chromatography and spectrometry applications

Abstract

A chromatography column assembly includes a standard column length of at least 100 mm and a plurality of columns connected in series. Each of the plurality of columns add up in length to the standard column length. Further disclosed are liquid chromatography systems including the described chromatography assemblies, and chromatographic methods deploying the described chromatographic assemblies.

IPC Classes  ?

• G01N 30/02 - Column chromatography
• G01N 30/56 - Packing methods or coating methods
• G01N 30/46 - Flow patterns using more than one column
• G01N 30/60 - Construction of the column

Abstract

An apparatus and method for locating and identifying an object comprises attaching an RFID tag around a cylindrical object. The RFID tag comprises a flexible circuit constructed and arranged for conformably positioning about a cylindrical object and a plurality of antenna loops serially positioned about the flexible circuit. The antenna loops are overlapped with each other so that the flexible circuit receives an RF signal at a strength for transmission to the flexible circuit at any orientation of the RFID tag about the cylindrical object. The RF signal is received by at least one of the plurality of overlapping antenna loops. The source receives from the RFID tag a reply signal comprising identification data corresponding to the RFID tag. An identity and location are determined for the cylindrical object in response to the reply signal.

IPC Classes  ?

• G06K 19/077 - Constructional details, e.g. mounting of circuits in the carrier
• G01S 13/74 - Systems using reradiation of radio waves, e.g. secondary radar systemsAnalogous systems
• H01Q 1/22 - SupportsMounting means by structural association with other equipment or articles

Abstract

The present disclosure describes an apparatus for performing electrical and rheology measurements of a material sample, comprising: an upper geometry; and a lower geometry. The lower geometry is stationary and the upper geometry rotates relative to the lower geometry. The lower geometry includes: a first electrode; a second electrode; and an electrical insulator for electrically isolating the first electrode from the second electrode. A thermally conductive metal plate is below the electrical insulator. A layer of low thermal impedance conductive material and a layer of electrically insulating material are between the electrical insulator and the thermally conductive metal plate.

IPC Classes  ?

• G01N 3/06 - Special adaptations of indicating or recording means
• G01N 3/22 - Investigating strength properties of solid materials by application of mechanical stress by applying steady torsional forces

Abstract

A chromatography column assembly includes a standard column length of at least 100 mm and a plurality of columns connected in series. Each of the plurality of columns add up in length to the standard column length. Further disclosed are liquid chromatography systems including the described chromatography assemblies, and chromatographic methods deploying the described chromatographic assemblies.

IPC Classes  ?

• G01N 30/60 - Construction of the column
• G01N 30/46 - Flow patterns using more than one column

Abstract

The present disclosure describes an apparatus for performing electrical and rheology measurements of a material sample, comprising: an upper geometry; and a lower geometry. The lower geometry is stationary and the upper geometry rotates relative to the lower geometry. The lower geometry includes: a first electrode; a second electrode; and an electrical insulator for electrically isolating the first electrode from the second electrode. A thermally conductive metal plate is below the electrical insulator. A layer of low thermal impedance conductive material and a layer of electrically insulating material are between the electrical insulator and the thermally conductive metal plate.

IPC Classes  ?

• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane
• G01N 11/16 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by measuring damping effect upon oscillatory body

Goods & Services

(1) Recorded or downloadable software for collecting, analyzing, storing, and reporting chromatography instrument utilization data; recorded or downloadable software for laboratory asset management, laboratory resource management, laboratory workflow management and analysis, analysis of utilization of laboratory instruments; recorded or downloadable software for validating laboratory processes.

Abstract

A valve for a liquid chromatography system comprises a stator; a rotor that rotates about a vertical axis relative to the stator, the rotor comprising a rotor body and a rotary shaft interface, the rotary shaft interface comprising a cavity along the vertical axis and an indentation at a bottom region of the cavity along the vertical axis; a platen in communication with a bottom surface of the rotor body, the platen having a conical feature along the vertical axis; and a ball bearing having a top portion positioned in the conical feature of the platen and a bottom portion positioned in the indentation of the rotary shaft interface allowing for pivoting of the platen relative to the vertical axis and for aligning a top surface of the rotor body to be parallel with a surface of the stator.

IPC Classes  ?

• G01N 30/20 - Injection using a sampling valve
• F16K 3/04 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with flat sealing facesPackings therefor with pivoted closure members
• G01N 30/02 - Column chromatography

Abstract

The present technology is directed to methods of preparing affinity chromatographic columns using streptavidin-conjugated particles and biotinylated affinity agents (i.e., biotinylated oligonucleotides, biotinylated antibodies or biotinylated antigen-binding fragments thereof). The methods of the present technology can be utilized to perform affinity capture assays in a high-throughput, efficient manner. Further, the affinity chromatographic columns disclosed herein are customizable due to the ability to utilize any biotinylated affinity agent. The methods herein utilize an on-line loading (i.e., loading the column with biotinylated affinity agent via a liquid chromatography system).

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/74 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving hormones

Abstract

A valve (200) for a liquid chromatography system comprises a stator (204); a rotor that rotates about a vertical axis (A) relative to the stator, the rotor comprising a rotor body (203) and a rotary shaft interface (211), the rotary shaft interface comprising a cavity (226) along the vertical axis and an indentation (221) at a bottom region of the cavity along the vertical axis; a platen (220) in communication with a bottom surface of the rotor body, the platen having a conical feature (227) along the vertical axis; and a ball bearing (222) having a top portion positioned in the conical feature of the platen and a bottom portion positioned in the indentation of the rotary shaft interface allowing for pivoting of the platen relative to the vertical axis and for aligning a top surface (207) of the rotor body to be parallel with a surface (209) of the stator.

IPC Classes  ?

• F16K 3/08 - Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with flat sealing facesPackings therefor with pivoted closure members in the form of closure plates arranged between supply and discharge passages with circular closure plates rotatable around their centres
• F16K 11/074 - Multiple-way valves, e.g. mixing valvesPipe fittings incorporating such valvesArrangement of valves and flow lines specially adapted for mixing fluid with all movable sealing faces moving as one unit comprising only sliding valves with pivoted closure members with flat sealing faces
• F16K 3/314 - Forms or constructions of slidesAttachment of the slide to the spindle
• G01N 30/20 - Injection using a sampling valve
• F16K 25/00 - Details relating to contact between valve members and seats

Abstract

The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C09D 143/04 - Homopolymers or copolymers of monomers containing silicon
• C23C 16/56 - After-treatment
• G01N 30/60 - Construction of the column

Abstract

A system and method for performing staggered separations are described. The system includes a plurality of solvent pumps; a plurality of proportioning valves each being in fluid communication with one of the solvent pumps, each proportioning valve having an inlet to receive a solvent from the respective one of the solvent pumps and having a plurality of outlets, wherein the proportioning valves are configured to control a flow of the solvent from each of the outlets; and a plurality of separation devices each in fluid communication with one of the outlets. Each of the separation devices receives a solvent mixture having a composition defined by the flows of the solvents from each of the respective outlets.

IPC Classes  ?

• G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
• G01N 30/02 - Column chromatography
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient

Abstract

A system and method for performing staggered separations are described. The system includes a plurality of solvent pumps; a plurality of proportioning valves each being in fluid communication with one of the solvent pumps, each proportioning valve having an inlet to receive a solvent from the respective one of the solvent pumps and having a plurality of outlets, wherein the proportioning valves are configured to control a flow of the solvent from each of the outlets; and a plurality of separation devices each in fluid communication with one of the outlets. Each of the separation devices receives a solvent mixture having a composition defined by the flows of the solvents from each of the respective outlets.

IPC Classes  ?

• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/46 - Flow patterns using more than one column
• G01N 30/32 - Control of physical parameters of the fluid carrier of pressure or speed

Abstract

Novel reagents comprising MS active, fluorescent compounds having an activated functionality for reaction with aldehydes and useful in labeling biomolecules such as glycans and methods of making the same are taught and described.

IPC Classes  ?

• G01N 33/533 - Production of labelled immunochemicals with fluorescent label
• C07D 215/48 - Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen

Abstract

The present disclosure is directed to stationary phase materials for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified silica particle stationary phase materials, which are optionally also methoxy-terminated polyethylene glycol surface modified.

IPC Classes  ?

• B01J 20/289 - Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
• B01J 20/10 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
• B01J 20/26 - Synthetic macromolecular compounds
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties

Abstract

A sample vial adapter for use in liquid chromatography includes a body structure extending between a top end and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body such that the channel at least partially encircles the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including an extension reducing a width of the channel opening, at least one of the extension and the body structure configured to elastically deform during the process of receiving the sample vial.

IPC Classes  ?

• G01N 30/18 - Injection using a septum or microsyringe
• B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus

Abstract

A sample vial adapter for use in liquid chromatography includes a body structure extending between a top end and a bottom end and defining a channel for receiving a sample vial having a cylindrical sample vial body such that the channel at least partially encircles the received sample vial, the body structure including a channel opening configured to receive the sample vial, the body structure including an extension reducing a width of the channel opening, at least one of the extension and the body structure configured to elastically deform during the process of receiving the sample vial.

IPC Classes  ?

• G01N 30/24 - Automatic injection systems

Abstract

Liquid chromatography systems described herein are smaller in size and with a reduced extra-column volume due to direct connection of a liquid chromatography column and/or detector to an injector valve and due to eliminating the need for a column oven by relying on heating of the liquid chromatography column and detector by a preheater. The size of the liquid chromatography systems are enough that the liquid chromatography systems may be deployed adjacent to automated sample preparation robotics, adjacent to a process stream, or adjacent to the inlet of a mass spectrometer. In addition, the extra-column volume of the liquid chromatography systems may be reduced by eliminating many of the connection tubes and by situating components of the liquid chromatography system in closer proximity.

IPC Classes  ?

• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• G01N 30/20 - Injection using a sampling valve
• G01N 30/46 - Flow patterns using more than one column
• G01N 30/72 - Mass spectrometers

Abstract

The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles.

IPC Classes  ?

• G01N 30/02 - Column chromatography
• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• B01J 20/282 - Porous sorbents
• B01J 20/286 - Phases chemically bonded to a substrate, e.g. to silica or to polymers
• G01N 21/53 - Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke

Abstract

The present disclosure is directed to hybrid-bonded siliceous materials. The materials of the present disclosure exhibit enhanced base stability and reduced baseline noise. As such, the materials disclosed herein are suitable for use across a range of chromatography applications, including size-exclusion chromatography and reversed-phase chromatography.

IPC Classes  ?

• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• B01D 15/20 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
• B01J 20/283 - Porous sorbents based on silica
• B01J 20/285 - Porous sorbents based on polymers
• B01J 20/286 - Phases chemically bonded to a substrate, e.g. to silica or to polymers
• B01J 20/30 - Processes for preparing, regenerating or reactivating
• B01J 20/32 - Impregnating or coating

Abstract

Disclosed herein are methods for the multiplexed characterization of analytes using dye-tagged affinity molecules. The DTAMs, useful in conjunction with liquid chromatography systems, allow for the parallel quantitation and characterization of attributes of one or more target analytes from complex solutions.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 27/622 - Ion mobility spectrometry
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The present disclosure is directed to hybrid-bonded siliceous materials. The materials of the present disclosure exhibit enhanced base stability and reduced baseline noise. As such, the materials disclosed herein are suitable for use across a range of chromatography applications, including size-exclusion chromatography and reversed-phase chromatography.

IPC Classes  ?

• B01J 20/289 - Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
• B01J 20/22 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising organic material
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• G01N 30/16 - Injection
• G01N 30/74 - Optical detectors

Abstract

Disclosed herein are methods for the multiplexed characterization of analytes using dye-tagged affinity molecules. The DTAMs, useful in conjunction with liquid chromatography systems, allow for the parallel quantitation and characterization of attributes of one or more target analytes from complex solutions.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A frit and a chromatography column that includes the frit are described. The frit is formed as a disk having a porosity, a radius and a thickness. The disk has a variation in porosity, thickness, or both porosity and thickness along a radial direction. A flow of a liquid through an outer region of the disk is advanced at an outlet end of the disk relative to a flow of the liquid through an inner region of the disk. The frit is configured to be disposed at one end of a column bed of a liquid chromatography column where the advance in the flow in the outer region relative to the flow in the inner region is predetermined to at least partially compensate for a radial variation in the linear velocity of the flow through the column bed.

IPC Classes  ?

• G01N 30/60 - Construction of the column
• G01N 30/26 - Conditioning of the fluid carrierFlow patterns
• G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups

Abstract

A frit and a chromatography column that includes the frit are described. The frit is formed as a disk having a porosity, a radius and a thickness. The disk has a variation in porosity, thickness, or both porosity and thickness along a radial direction. A flow of a liquid through an outer region of the disk is advanced at an outlet end of the disk relative to a flow of the liquid through an inner region of the disk. The frit is configured to be disposed at one end of a column bed of a liquid chromatography column where the advance in the flow in the outer region relative to the flow in the inner region is predetermined to at least partially compensate for a radial variation in the linear velocity of the flow through the column bed.

IPC Classes  ?

• G01N 30/60 - Construction of the column
• G01N 30/02 - Column chromatography

Abstract

Described herein are systems and methods used for washing needle assemblies with multiple needles.

IPC Classes  ?

• G01N 1/34 - PurifyingCleaning
• G01N 1/14 - Suction devices, e.g. pumpsEjector devices
• B01L 3/02 - BurettesPipettes
• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices

Goods & Services

Chemical test kits for indicating the presence or absence of toxins; chemical and biochemical reagents for non-medical purposes for indicating the presence or absence of toxins; chemically treated non-medical test strips for indicating the presence or absence of toxins; reagents for laboratory use; reagents for scientific and research use; analytical standard solutions and reagents for scientific and research use; analytical standard solutions and reagents for testing soil for the purpose of monitoring contaminants; chromatography chemicals; sample preparation kits for scientific and research purposes, comprising biological standards, namely, monoclonal antibodies, and chemical reagents for biological analysis; chemical reagents for preparing samples for chromatography and mass spectrometry for non-medical, laboratory use; chemicals, namely, buffers and standard solutions used in analytical chemistry; buffers in the nature of chemicals used in analytical chemistry; chemically-treated non-medical test strips and test-strip holders specifically adapted for, and sold with, the aforementioned test strips for the detection of mycotoxins by antibody affinity reactions in grain, feed, and food products; assay kits comprising an affinity chromatography column and chemical reagents for testing for mycotoxin contamination in grain, feed, and food products; environmental quality control fluid, gas, and soil samples for calculating standards for testing and verifying the quality of soil and air A house mark for a full line of analytical apparatus and instruments for laboratory use and their parts, fittings, and accessories therefor; A housemark for analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification; A housemark for laboratory instruments, namely, chromatography and spectrometry instruments for laboratory use; A housemark for a full line of material testing laboratory instruments; Laboratory apparatus and instruments for solid phase extraction; positive pressure manifolds for chromatography instruments; laboratory analytical instruments for detecting and measuring visual and ultraviolet light; laboratory analytical instruments for detecting and measuring fluorescence; laboratory analytical instruments, namely, ultraviolet and visual light sensors; laboratory analytical instruments, namely, photodiode array sensors; laboratory analytical instruments, namely, evaporative light scattering detectors; laboratory analytical instruments, namely, refractive index detectors; analytical instruments featuring light-guiding flow cell for performing and analyzing biological and chemical samples, not for medical use; wheeled carts specially adapted for laboratory instruments; laboratory instruments for organizing, storing, and processing laboratory samples in a temperature controlled environment; fluid temperature control system for laboratory use and scientific purposes; laboratory instrument for controlling sample temperature; laboratory instrument for controlling sample temperature for use in connection with laboratory automation systems; laboratory instruments for processing samples with liquid chromatography columns; laboratory instruments for heating and cooling laboratory samples and chromatography columns; chromatography columns for laboratory use featuring radio frequency identification (RFID) transponders; radio frequency identification (RFID) transponders and readers for use with chromatography columns; radio frequency radio frequency identification (RFID) transponders, tags, labels, and stickers sold as components of a chromatography column; analytical laboratory instruments for automating sample analyses, diluting samples, tracking and tracing results of sample processing; spectral detectors for use in connection with mass spectrometers and liquid chromatographs not for medical use; detectors for detecting and quantifying ultraviolet and visible spectral exposure for laboratory use; florescence detectors for analysis and detection of samples containing chemiluminescent properties for laboratory use; laboratory robots; laboratory robots used for the purpose of handling laboratory pipettes; laboratory pipettes; automated laboratory pipettes; electronic laboratory pipettes; automated pipetting instrument for laboratory use for use in connection with liquid chromatography or mass spectrometry; pipette racks for laboratory use; pipette tips for laboratory use; pipette accessories, namely, magnetic beads used for laboratory use for DNA and RNA extraction within pipette tips; laboratory equipment and apparatus, namely, magnetic beads for laboratory use for use in purifying genomic, plasmid and mitochondrial DNA; accessories for analytical apparatus and instruments for scientific and laboratory research purposes, namely, Peltier plates in the nature of electrical cooling and heating plates for cooling and heating laboratory samples; laboratory equipment, namely, orbital shakers for laboratory use; automated laboratory sample extraction instruments comprised of vacuum control valves and alignment posts; laboratory equipment and apparatus, namely, column tube grippers and microplate grippers for transfer of centrifuge tubes and microplates; laboratory equipment and supplies, namely, microfluidic chips for analyzing RNA and DNA; magnetic blocks for holding, organizing, agitating, stirring, heating, cooling, and storing consumables for laboratory use; laboratory equipment and supplies, namely, glass bottles, tanks, tubes, vials, and caps for tubes for laboratory use; laboratory equipment and supplies, namely, plastic tubes and bottles for laboratory use; plates for collecting and analyzing samples, namely, optimization plates, elution plates, photoredox plates, and well plates having multi-well arrays that can be used in chemical analysis, biological analysis or patterning for scientific or laboratory research use; non-medical immunostaining racks for scientific or laboratory purposes; disposable reusable dispenser syringes for laboratory sample use, not for medical use; laboratory instrument for measuring and locating the liquid levels of consumables for laboratory use in the nature of liquid level sensors; computer hardware for laboratory, scientific, and clinical use for use in managing laboratory operations and analyzing laboratory samples; electronic measurement systems, namely light scattering photometers, differential refractometers, online particle fractionators for use with light scattering photometers, sonication (non-chemical) optical flow cell cleaners; laboratory apparatus and instruments, namely multi-angel light scattering instruments; Laboratory apparatus and instruments, namely dynamic light scattering instruments; Laboratory apparatus and instruments, namely electrophoretic light scattering instruments; Laboratory apparatus and instruments, namely field-flow fractionation instruments; Laboratory analytical instruments for measuring and characterizing molecular molar mass, molecular size, molecular charge, molecular interaction, molecular shape and confirmation, and molecular conjugation and bonding; computer laboratory workstations comprised of downloadable and recorded software for scientific, clinical, and diagnostic laboratory use, namely, for use in managing laboratory operations, analyzing laboratory samples, and automating laboratory experiments; downloadable or recorded application programming interface (API) software; downloadable or recorded software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment; downloadable or recorded software for analyzing samples for compliance with environmental regulations; downloadable or recorded software for analyzing samples for toxins or contaminants; downloadable or recorded software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; downloadable or recorded software for automating laboratory instruments; downloadable or recorded software for automating pipetting; downloadable or recorded software for calibrating pipettes; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; downloadable or recorded software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; downloadable or recorded software for controlling chromatography and spectrometry instruments; downloadable or recorded software for controlling laboratory instruments; downloadable or recorded software for creating and executing laboratory methods and protocols; downloadable or recorded software for data analysis for laboratory instruments and laboratory data; downloadable or recorded software for facilitating laboratory compliance with government regulation; downloadable or recorded software for identifying and analyzing the components of a sample; downloadable or recorded software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; downloadable or recorded software for laboratory and laboratory sample management; downloadable or recorded software for laboratory informatics; downloadable or recorded software for laboratory robot control; downloadable or recorded software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; downloadable or recorded software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; downloadable or recorded software for operating laboratory instruments; downloadable or recorded software for purification of samples in chromatography and spectrometry applications; downloadable or recorded software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; downloadable or recorded software for validating pipette performance; downloadable or recorded software for validating processes in chromatography and mass spectrometry laboratories; downloadable or recorded software for validating processes in laboratories; analytical apparatus and instruments for performing biological testing, physical testing, biological and chemical separation, purification, screening and identification and structural parts and structural fittings therefor, namely, liquid chromatography apparatus and instruments for laboratory use and structural parts and structural fittings therefor, gas chromatography apparatus for laboratory use and structural parts and structural fittings therefor, ion mobility chromatography apparatus for laboratory use and structural parts and structural fittings therefor, mass spectrometry apparatus and instruments for laboratory use and structural parts and structural fittings therefor; analytical apparatus and instruments, namely, detectors for indicating the presence or absence of toxins in the fields of agriculture and food processing and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, magnetic suspension balances and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, mechanical testing instruments, rubber testing instruments and structural parts and structural fittings therefor; scientific analytical apparatus and instruments in the nature of magnetic suspension balances for isothermal measuring equilibrium and kinetics of ad- or absorption processes and for thermogravimetric measurements at high temperatures and high pressures, gravimetric analysis, thermogravimetric analysis or sorption analysis and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use for gas and vapor dosing, mixing, and pressure control and structural parts and structural fittings therefor; analytical apparatus and instruments for laboratory use, namely, electrosurgical instruments used with analytical instruments to identify the presence or absence of toxins in sample tissues, electrosurgical smoke evaluation devices, devices for identification of chemicals in electrosurgical smoke for non-medical diagnostic and analytical purposes; fluorometers and structural parts and structural fittings therefor; lateral flow readers and structural parts and structural fittings therefor; rheometers and structural parts and structural fittings therefor; calorimeters and structural parts and structural fittings therefor; dilatometers and structural parts and structural fittings therefor; laboratory chemical reactors; diagnostic apparatus for testing food; diagnostic apparatus for testing agricultural products; diagnostic apparatus for the detection and identification of microbes and pathogens for laboratory or research use; diagnostic apparatus for testing for illegal drugs, diagnostic apparatus for forensic toxicology; chromatography columns for laboratory use; laboratory consumables, namely, vials, containers, sample trays, sample plates, sample containers, tubing, cartridges, pipettes; solid phase extraction cartridges and plates for laboratory use; affinity columns, comprising monoclonal antibodies, for purifying samples for subsequent liquid-chromatography and mass-spectrometry testing for mycotoxin contamination in grain, feed, and food products; computer operating software for analytical apparatus and instruments; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the materials; computer operating software for bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment; analytical apparatus and instruments for laboratory use, namely, flash diffusivity analyzers and parts thereof; mechanical testing instruments, for testing the mechanical or material properties of test samples; mass spectrometer and parts thereof; analytical apparatus and instruments for analyzing mechanical or material properties of material samples for scientific research purposes, namely, thermal analyzers, thermal conductivity meters; laboratory instruments, namely, specimen testing instruments used to apply cyclic and static loads to a specimen integrated with a controller used for fatigue testing and material testing of the specimen subjected to loads; rheometers for measuring the viscosity and viscoelasticity of fluids, and parts and therefor; laboratory chemical reactors and parts thereof; laboratory instruments, namely, autosamplers; laboratory instruments, namely, pressure cells in the nature of vessels that can withstand high internal pressures; computer operating software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment; accessories for use with analytical apparatus and instruments for scientific research purposes, namely, grips and platens, stent and graft testers, cylinders, equipment for cutting specimens, and temperature control units; accessories for analytical apparatus and instruments for scientific research purposes, namely, peltier plate systems in the nature of electrical heating and cooling plates, plate pressure cells in the nature of vessels that can withstand high internal pressures, sensors for sensing the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression, electrically heated cylinders in the nature of test chambers, relative humidity sensors, optical plates, modular microscope accessory in the nature of microscopes, environmental test chambers, namely, temperature simulation equipment, small angle light scattering diodes, interfacial rheology accessories, namely, accessories for analyzing viscoelasticity, tribo-rheometry accessories, namely, accessories for analyzing friction, magneto-rheology accessories, namely, accessories for analyzing properties of materials subject to magnetic fields, electro-rheology accessories, namely, accessories for analyzing properties of materials subject to electric currents, UV curing accessories, namely, accessories for analyzing properties of materials subject to ultraviolet radiation, dieletric measurement accessories, namely, accessories for analyzing properties of materials subject to an oscillating electrical field, diaelectric thermal analysis accessories, namely, accessories for analyzing properties of materials subject to controlled temperatures and an oscillating electrical field, immobilization cells, namely, test chambers for characterizing the drying, retention, and immobilization kinetics of paints, coatings, and slurries; starch pasting cells, namely, test chambers for analyzing starches, dynamic mechanical analysis accessories, namely, accessories for analyzing material properties of solid materials, refrigerated cooling systems, namely, analytical instrument accessories for controlling sample temperature, laboratory instruments, namely, autosamplers and automated sample processing apparatus and carousels, forced convection oven accessories, namely, analytical instrument accessories for controlling sample temperature, orthogonal superposition accessories, namely, accessories for analyzing nonlinear viscoelasticity, 2-dimensional small amplitude oscillatory shear accessories, namely, accessories for analyzing nonlinear viscoelasticity, extensional viscosity fixture accessories, namely, accessories for analyzing extensional viscosity and stress, cone and partitioned plate accessories, namely, accessories for analyzing elastic materials, interfacial rheology accessories, degassing stations, namely, instruments for removing gasses from samples, clamps for use with analytical instruments, evacuated ampoules made of glass, metal, or plastic for use in testing and analyzing samples for non-medical purposes, sample measuring system accessories, namely, accessories for measuring the mechanical and material properties of samples, temperature, pressure, motion, distances, force, torque, viscosity, elasticity, material strain, tension, deflection, compression Promoting the exchange of information and resources within the laboratory research community to achieve advances in the field of the separation sciences; Promoting the exchange of information and resources within the laboratory research community to achieve advances in the fields of chemistry, biochemistry, pharmaceuticals, environmental science, material science, and material characterization; business consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, forensic toxicology, food science, environmental science, material science, and material characterization; Providing facilities for business meetings; online, catalog, and mail order services featuring analytical apparatus and instruments for scientific and research use; online, catalog, and mail order services featuring computer operating software for analytical apparatus and instruments, analyzing and manipulating data; online, catalog, and mail order services featuring computer operating software data acquisition, processing, and reporting Providing facilities for scientific research in the nature of a laboratory for the separation sciences; providing facilities for scientific research in the nature of a laboratory featuring instruments for chromatography, mass spectrometry, and material science; consulting services in the field of laboratory research for chemistry, biochemistry, pharmaceutical science, material science, and material characterization; consulting services in the field of laboratory science; technical consulting services in best practices for laboratory research in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; Scientific research and development; laboratory study and research in the fields of chemistry, biochemistry, pharmaceutical science, material science, and material characterization; scientific laboratory research services in the field of chemistry, biochemistry pharmaceutical science, material science and material characterization; research and development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization; advisory services relating to the fields of development of laboratory instruments in the field of chemistry, biochemistry pharmaceutical science, material science, and material characterization advisory services related to material testing; Updating, repair, and maintenance of laboratory software; Technical support services, namely, providing information, consultation and diagnostic services in the fields of electronic and optical components, computer software, and scientific, electronic test, measurement, biological and chemical analytical, and optical equipment; services for updating and maintenance of laboratory instrument and laboratory management computer software; technical support services, namely, troubleshooting of laboratory computer software problems; technical support services, namely, operational qualification services for scientific analytical apparatus and instruments, specifically, technical verification and validation of laboratory equipment; providing technical advice relating to use, operation, and function of scientific analytical apparatus and instruments; software as a service (SAAS) services featuring software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment software as a service (SAAS) services featuring software for analyzing samples for compliance with environmental regulations; software as a service (SAAS) services featuring software for analyzing samples for toxins or contaminants; software as a service (SAAS) services featuring software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; software as a service (SAAS) services featuring software for automating laboratory instruments; software as a service (SAAS) services featuring software for automating pipetting; software as a service (SAAS) services featuring software for calibrating pipettes; software as a service (SAAS) services featuring software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; software as a service (SAAS) services featuring software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; software as a service (SAAS) services featuring software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; software as a service (SAAS) services featuring software for controlling chromatography and spectrometry instruments; software as a service (SAAS) services featuring software for controlling laboratory instruments; software as a service (SAAS) services featuring software for creating and executing laboratory methods and protocols; software as a service (SAAS) services featuring software for data analysis for laboratory instruments and laboratory data; software as a service (SAAS) services featuring software for facilitating laboratory compliance with government regulation; software as a service (SAAS) services featuring software for identifying and analyzing the components of a sample; software as a service (SAAS) services featuring software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; software as a service (SAAS) services featuring software for laboratory and laboratory sample management; software as a service (SAAS) services featuring software for laboratory informatics; software as a service (SAAS) services featuring software for laboratory robot control; software as a service (SAAS) services featuring software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; software as a service (SAAS) services featuring software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; software as a service (SAAS) services featuring software for operating laboratory instruments; software as a service (SAAS) services featuring software for purification of samples in chromatography and spectrometry applications; software as a service (SAAS) services featuring software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; software as a service (SAAS) services featuring software for validating pipette performance; software as a service (SAAS) services featuring software for validating processes in chromatography and mass spectrometry laboratories; software as a service (SAAS) services featuring software for validating processes in laboratories; providing online, non-downloadable software for analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment; providing online, non-downloadable software for analyzing samples for compliance with environmental regulations; providing online, non-downloadable software for analyzing samples for toxins or contaminants; providing online, non-downloadable software for automating chromatography and mass spectrometry operations in a chromatography and mass spectrometry apparatus; providing online, non-downloadable software for automating laboratory instruments; providing online, non-downloadable software for automating pipetting; providing online, non-downloadable software for calibrating pipettes; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data for use in scientific analysis; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting chromatography and mass spectrometry data; providing online, non-downloadable software for collecting, analyzing, organizing, storing, and reporting data from analytical equipment in the chemical, pharmaceutical, life sciences, environmental, clinical diagnostic, food science and safety, and forensic toxicology fields; providing online, non-downloadable software for controlling chromatography and spectrometry instruments; providing online, non-downloadable software for controlling laboratory instruments; providing online, non-downloadable software for creating and executing laboratory methods and protocols; providing online, non-downloadable software for data analysis for laboratory instruments and laboratory data; providing online, non-downloadable software for facilitating laboratory compliance with government regulation; providing online, non-downloadable software for identifying and analyzing the components of a sample; providing online, non-downloadable software for laboratory analytical method development, testing, and validation management, namely, for creating, testing, and validating methods for using analytical instruments for laboratory use; providing online, non-downloadable software for laboratory and laboratory sample management; providing online, non-downloadable software for laboratory informatics; providing online, non-downloadable software for laboratory robot control; providing online, non-downloadable software for laboratory, scientific, and clinical use, namely, computer software for use in managing laboratory operations and analyzing laboratory samples; providing online, non-downloadable software for managing laboratory workflow, namely, software for controlling, organizing, and tracking laboratory testing projects, laboratory test results, laboratory staff utilization, and reagent and sample inventories; providing online, non-downloadable software for operating laboratory instruments; providing online, non-downloadable software for purification of samples in chromatography and spectrometry applications; providing online, non-downloadable software for use in connection with light scattering and refractive index detection, data acquisition applications, and liquid chromatographic separations; providing online, non-downloadable software for validating pipette performance; providing online, non-downloadable software for validating processes in chromatography and mass spectrometry laboratories; providing online, non-downloadable software for validating processes in laboratories; design and development of analytical apparatus and instruments and parts therefor for scientific and research use; design and development of chemistry consumables for laboratory use; design and development of computer software for analytical apparatus and instruments or scientific and research use; scientific consulting services in the field of analytical chemistry; scientific consulting services in the field of analytical chemistry in the nature of product qualification and validation, implementation project management of scientific research for chemical analyses, computer software testing for chemical analyses, laboratory analytics, and informatics for chemical analyses; scientific consulting in the field of analytical chemistry, namely, development of practical applications and usage of laboratory apparatus and instruments for use in research in analytical chemistry; cloud computing featuring software and software as a service featuring software for use with analytical apparatus and instruments in bioinformatics, laboratory data analysis, analysis and visualization of data from liquid chromatography and mass spectrometry instruments, discovery, identification, and analysis of chemical compounds, analyzing materials by measuring changes in the physical or chemical properties of the material as the material is subjected to controlled changes in its environment; maintenance and repair services for database systems and computer operating software

Abstract

The present technology provides a method of eluting an analyte of interest from an immunoaffinity column. The methods of the present technology provide a simplified method for disassociating a bound analyte from the immunoaffinity column and allows for direct downstream processing of the eluate.

IPC Classes  ?

• G01N 1/40 - Concentrating samples
• G01N 30/02 - Column chromatography
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Goods & Services

Recorded or downloadable software for collecting, analyzing, storing, and reporting chromatography instrument utilization data; Recorded or downloadable software for laboratory asset management, laboratory resource management, laboratory workflow management and analysis, analysis of utilization of laboratory instruments; Recorded or downloadable software for validating laboratory processes

Abstract

The present technology provides a method of eluting an analyte of interest from an immunoaffinity column. The methods of the present technology provide a simplified method for disassociating a bound analyte from the immunoaffinity column and allows for direct downstream processing of the eluate.

IPC Classes  ?

• G01N 30/54 - Temperature
• G01N 30/02 - Column chromatography
• G01N 30/30 - Control of physical parameters of the fluid carrier of temperature
• G01N 33/02 - Food

Abstract

The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

IPC Classes  ?

• G01N 30/72 - Mass spectrometers
• C23C 14/04 - Coating on selected surface areas, e.g. using masks
• C23C 14/12 - Organic material
• G01N 30/02 - Column chromatography

Abstract

An apparatus for performing electrical and rheology measurements of a material sample, comprises a first plate; a second plate; a rotatable drive shaft extending from a motor to rotate the first plate relative to the second plate; and a sample gap between the first plate and the second plate. The second plate includes first and second electrodes that receive a voltage and form an electric field at the sample gap.

IPC Classes  ?

• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane
• G01N 27/04 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
• G01N 27/06 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid

Abstract

Described is an apparatus for measuring rheological and electrical properties of a sample. The apparatus includes a first geometry comprising a first and a second stationary element each being electrically conductive and having a surface disposed to face the surface of the other stationary element across a gap. The first and second stationary elements are electrically isolated from each other. The apparatus further includes a second geometry comprising a rotatable element disposed in the gap and configured to rotate between the first and second stationary elements. The rotatable element is electrically conductive such that a first electric field is generated between the first stationary element and the rotatable element and a second electric field is generated between the rotatable element and the second stationary element in response to application of a voltage difference applied across the first and second stationary elements.

IPC Classes  ?

• G01N 11/14 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by moving a body within the material by using rotary bodies, e.g. vane

Abstract

The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

IPC Classes  ?

• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01D 15/12 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
• B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
• B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 3/02 - BurettesPipettes

Abstract

The present technology is directed to methods of improved nucleic acid-based ion-exchange chromatography. In particular, the methods utilize a modification of a sample, and in some examples a modification of mobile phase carrying the sample to the ion-exchange column. In a method of the technology, a bracketed sample is loaded into an injector needle apparatus and injected into an ion-exchange column. The bracketed sample is loaded by (a) adding a first-plug solvent comprising a first salt and having a basic pH; (b) adding a sample plug; and (c) adding a second-plug solvent comprising a second salt and having a basic pH. In certain examples, the bracketed sample is delivered to the column using a mobile phase gradient consisting of first solvent and second solvent and wherein 0% of the second solvent is excluded from the gradient.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present technology provides a description of a flow column dampener device capable of decreasing the MALS noise from solvent contaminants and transient ripples induced by the HPLC pump prior to the evaluation and characterization of the biophysical properties of macromolecules. This dampener typically includes a particle bed column that can trap and remove the noise-inducing soluble particulates from mobile phase, while simultaneously dampening the noise induced by pump-induced ripples. The types of sorbents, sorbent particle size range and column size formats of the device provides significant reduction in MALS noise prior to the evaluation. This device enables confident characterization of cell and gene therapy products with accurate measurements of the physical properties of the macromolecule analytes such as cell and gene therapy products.

IPC Classes  ?

• B01D 15/34 - Size-selective separation, e.g. size-exclusion chromatographyGel filtrationPermeation
• G01N 21/47 - Scattering, i.e. diffuse reflection

Abstract

In various aspects, the present disclosure pertains to sorbents for isolating at least one target protein from a liquid sample, the sorbents comprising a solid support comprising attached at least one attached high affinity reagent with an affinity for the at least one target protein. Other aspects of the present disclosure include kit that contain such sorbents and methods of treating samples using the same.

IPC Classes  ?

• C07K 1/30 - ExtractionSeparationPurification by precipitation
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

The present technology is directed to a method and kit for extracting oligonucleotides using solid phase extraction. Oligonucleotides are extracted from a biological sample by using a protease-assisted sample pretreatment step and a subsequent weak anion exchange solid phase extraction process.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 9/64 - Proteinases derived from animal tissue, e.g. rennin