Provided herein is an aqueous nucleic acid purification buffer comprising a polar aprotic solvent. Also provided is use of such a purification buffer to precipitate a nucleic acid from solution to a solid support, thereby providing a nucleic acid bound solid support. A method of processing a nucleic acid is also provided, comprising exposing a sample comprising the nucleic acid to an aqueous medium comprising a polar aprotic solvent in the presence of a solid support; and precipitating the nucleic acid to the solid support, thereby providing a nucleic acid bound solid support. Further provided are kits and compositions comprising such an aqueous nucleic acid purification buffer, as well as related nucleic acid analysis apparatus, and use of said kits.
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
Particles This invention relates to magnetic clusters comprising nanocrystals of iron oxide. The magnetic clusters comprise an aromatic carboxylic acid and/or the magnetic clusters are monodisperse. The magnetic clusters may be useful in biological assays and other applications. The invention also relates to processes for preparing such magnetic clusters and methods of using the magnetic clusters, as well kits comprising the magnetic clusters.
A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.
The invention relates to polymer-interaction molecule (e.g., antibodies) conjugates and uses of such conjugates for delivery of interaction molecules to cells. The invention also relates uses polymer-interaction molecule (e.g., antibodies) conjugates for eliciting cellular responses (e.g., induction of cell proliferation).
C08F 283/04 - Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass on to polycarbonamides, polyesteramides or polyimides
6.
Methods and compositions for coupling nucleic acid to a functionalized support
This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.
This invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.6; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse coated hydrogel polymer particles.
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 228/02 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a bond to sulfur or by a heterocyclic ring containing sulfur by a bond to sulfur
C08F 230/02 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing phosphorus
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 8/32 - Introducing nitrogen atoms or nitrogen-containing groups by reaction with amines
C08F 8/34 - Introducing sulfur atoms or sulfur-containing groups
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
The invention relates to polymer-interaction molecule (e.g., antibodies) conjugates and uses of such conjugates for delivery of interaction molecules to cells. The invention also relates uses polymer-interaction molecule (e.g., antibodies) conjugates for eliciting cellular responses (e.g., induction of cell proliferation).
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
A61K 47/60 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
A61K 47/68 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
A61K 47/69 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Sub-micron magnetic particles, consisting of,
superparamagnetic iron oxide for use in medical and
scientific research. Sub-micron magnetic particles, consisting of,
superparamagnetic iron oxide for therapeutic use, as well as
for diagnosis for medical purposes for in vitro use.
11.
SELECTIVE EXPANSION OF DIFFERENT SUBPOPULATIONS OF T CELLS BY THE ALTERATION OF CELL SURFACING SIGNALS AND SIGNAL RATIO
This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.
A magnetic particle separation system includes a magnetic field generating device having an upper surface with a receiving area formed thereon; and a magnetic field generating element disposed beneath the upper surface, the magnetic field generating element being configured to produce a magnetic field above the upper surface. A container assembly is disposed on the upper surface and includes: a flexible container having an outer wall with an interior surface that at least partially bounds an internal compartment, the outer wall having a front side and an opposing back side with the internal compartment disposed therebetween; a fluid inlet extending through the outer wall at the front side; a fluid outlet extending through the outer wall at the front side; and a first partition projecting into the internal compartment from the front side between the fluid inlet and the fluid outlet.
B03C 1/30 - Combinations with other devices, not otherwise provided for
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
in vitro transcriptionin vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.
LIFE TECHNOLOGIES HOLDINGS PTE LIMITED (Singapore)
Inventor
Luhr, Morten
Gordon, Michael
Sia, Ming Tiong
Chong, Kok Shyong
Soh, Woon Liang Terence
Lim, Seng Leong
Schroeder, Ida Caroline
Alfsnes, Katrine
Lim, Terry Jianhui
Bosnes, Marie
Neurauter, Axl Alois
Pedersen, Ketil Winther
Wilbur, Autumn Day
Zimmerman, Sean
Abstract
A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.
This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.
C08F 265/10 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of amides or imides
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 8/34 - Introducing sulfur atoms or sulfur-containing groups
C08F 8/32 - Introducing nitrogen atoms or nitrogen-containing groups by reaction with amines
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.
C08F 265/10 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of amides or imides
Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25− T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.
oct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse magnetic hydrogel polymer particles and monodisperse coated polymer particles.
C08L 33/26 - Homopolymers or copolymers of acrylamide or methacrylamide
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 228/02 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a bond to sulfur or by a heterocyclic ring containing sulfur by a bond to sulfur
C08F 230/02 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing phosphorus
G01N 33/567 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent utilising isolate of tissue or organ as binding agent
C08B 37/00 - Preparation of polysaccharides not provided for in groups Derivatives thereof
G01N 33/532 - Production of labelled immunochemicals
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
G01N 33/554 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
C07D 403/12 - Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a chain containing hetero atoms as chain links
This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
23.
Hydrophilic polymeric particles and methods for making and using same
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 8/34 - Introducing sulfur atoms or sulfur-containing groups
C08F 8/32 - Introducing nitrogen atoms or nitrogen-containing groups by reaction with amines
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
26.
Methods and compositions for nucleic acid isolation
The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.
Methods of separating magnetic particles from a fluid include introducing a fluid mixture that includes a liquid media, a biological component, and magnetic particles into an internal compartment of a container through an inlet, allowing at least a portion of the mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet on the opposite side of the partition, and applying a magnetic field to the mixture in the internal compartment so that the magnetic particles are retained within the container or internal compartment thereof by the magnetic field. The partition is formed by permanently or reversibly securing upper and lower container walls together, such as by welding or pressing, between the inlet and the outlet such that the media and biological component must flow around the partition and through the magnetic field to exit the internal compartment through the outlet.
A61M 1/36 - Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
28.
Polymer substrates formed from carboxy functional acrylamide
A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non-crosslinked oligomers of poly N,N-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.
C08F 265/10 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of amides or imides
This invention relates to monodisperse magnetic hydrogel polymer particles comprising a magnetic material and a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse magnetic hydrogel polymer particles and monodisperse coated polymer particles.
C08F 265/10 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of amides or imides
C08L 33/26 - Homopolymers or copolymers of acrylamide or methacrylamide
This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide- oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.
The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves the use of an organic solvent with sufficient density so that centrifugation of the sample is not needed to achieve phase separation.
This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (CV) % of the particles, when measured by CPS disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. This invention also relates to monodisperse polymer particles for use as seed particles in the Ugelstad process.
B32B 5/16 - Layered products characterised by the non-homogeneity or physical structure of a layer characterised by features of a layer formed of particles, e.g. chips, chopped fibres, powder
B32B 9/00 - Layered products essentially comprising a particular substance not covered by groups
C08F 4/46 - MetalsMetal hydridesMetallo-organic compoundsUse thereof as catalyst precursors selected from light metals, zinc, cadmium, mercury, copper, silver, gold, boron, gallium, indium, thallium, rare earths, or actinides selected from alkali metals
Methods of separating magnetic particles from a fluid include introducing a fluid mixture that includes a liquid media, a biological component, and magnetic particles into an internal compartment of a container through an inlet, allowing at least a portion of the mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet on the opposite side of the partition, and applying a magnetic field to the mixture in the internal compartment so that the magnetic particles are retained within the container or internal compartment thereof by the magnetic field. The partition is formed by permanently or reversibly securing upper and lower container walls together, such as by welding or pressing, between the inlet and the outlet such that the media and biological component must flow around the partition and through the magnetic field to exit the internal compartment through the outlet.
This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.
This invention relates, inter alia, to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Chemical reagents for use in laboratory analyses and cell preparation; diagnostic preparations for scientific use; chemical preparations for analytical use in scientific laboratories; immunoglobulin preparations for analytical use in scientific laboratories; all for sale separately or in kit form. Chemical reagents for use in medical laboratory analyses, cell preparation, and in vitro testing; diagnostic preparations for medical use; chemical preparations for diagnostic use in medical laboratories; diagnostic preparation, namely, immunoglobulin preparations for medical use.
This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non- crosslinked oligomers of poly Ν,Ν-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.
C08F 2/38 - Polymerisation using regulators, e.g. chain terminating agents
C08F 265/10 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of amides or imides
39.
Polymer substrates formed from carboxy functional acrylamide
A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
40.
Conjugation of carboxyl functional hydrophilic beads
A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.
C07F 7/10 - Compounds having one or more C—Si linkages containing nitrogen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 236/20 - Copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds unconjugated
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12M 1/42 - Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
47.
HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
48.
HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08J 9/20 - Making expandable particles by suspension polymerisation in the presence of the blowing agent
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of monomer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Microspheres made from plastics, namely magnetizable polymer beads for use in medical and scientific research. Microspheres made from plastic, namely magnetizable polymer beads for therapeutic use, as well as for diagnosis for medical purposes for in vitro use.
A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.
C07F 7/10 - Compounds having one or more C—Si linkages containing nitrogen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 236/20 - Copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds unconjugated
This invention relates to a sample holder for isolating magnetically labelled particles from a non-magnetic medium in a plurality of samples. The holder comprises a magnetic base for applying a magnetic force to the magnetically labelled particles and a body which is mountable on the base and demountable therefrom. The body comprises an array of sample holding portions and a magnetizable member which is magnetically urged towards the magnetic base when the body is seated on the base, whereby the body is urged to remain seated on the base. The invention also relates to use of the sample holder to separate magnetic particles from a non-magnetic medium and methods of performing such a separation.
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.
C07F 7/10 - Compounds having one or more C—Si linkages containing nitrogen
C08L 9/00 - Compositions of homopolymers or copolymers of conjugated diene hydrocarbons
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 236/20 - Copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds unconjugated
A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 236/20 - Copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds unconjugated
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
62.
SAMPLE HOLDER WITH MAGNETIC BASE AND MAGNETISABLE BODY
This invention relates to a sample holder for isolating magnetically labelled particles from a non-magnetic medium in a plurality of samples. The holder comprises a magnetic base for applying a magnetic force to the magnetically labelled particles and a body which is mountable on the base and demountable therefrom. The body comprises an array of sample holding portions and a magnetisable member which is magnetically urged towards the magnetic base when the body is seated on the base, whereby the body is urged to remain seated on the base. The invention also relates to use of the sample holder to separate magnetic particles from a non-magnetic medium and methods of performing such a separation.
A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
C08F 230/08 - Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 8/34 - Introducing sulfur atoms or sulfur-containing groups
C07F 7/18 - Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
C08F 130/08 - Homopolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing a metal containing silicon
C08F 2/20 - Suspension polymerisation with the aid of macromolecular dispersing agents
C08F 2/26 - Emulsion polymerisation with the aid of emulsifying agents anionic
C08F 220/58 - Amides containing oxygen in addition to the carbonamido oxygen
Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25− T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.
A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.
The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3). The disclosure further relates to a kit of parts and a method for isolating magnetically labelled particles from a non-magnetic medium using the magnetic separation device.
The disclosure relates to a magnetic separation rack for isolating magnetically labeled particles from a non-magnetic medium comprising a body portion (1) and a foot portion (8). The body portion comprises an array of sample vessel retaining portions (2) and plurality of magnetizing portions (3). Each sample vessel retaining portion comprises at least one visible portion such that when a sample vessel is mounted in a sample vessel retaining portion at least one portion of the sample vessel is visible to a user. The magnetizing portions are arranged within the body portion (1) such that at least two magnetizing portions (3) are circumferentially spaced about each sample vessel retaining portion (2). The foot portion is pivotally coupled to the body portion such that the body portion is operatively tiltable with respect to the foot portion such that each sample vessel retaining portion may retain a sample vessel mounted therein in a tilted position with respect to the vertical. The disclosure further relates to a method of isolating magnetically labeled particles from a non-magnetic medium using the said magnetic separation rack.
Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.
This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1µm, wherein the particles are solid or porous, and wherein the coefficient of variation (CV)% of the particles, when measured by CPS disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. This invention also relates to monodisperse polymer particles for use as seed particles in the Ugelstad process.
The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.
A process for the preparation of coated polymer particles containing superparamagnetic crystals, said process comprising reacting surface-functionalized, superparamagnetic crystal-containing polymer particles of diameter less than 0.5 μm with at least one polyisocyanate and at least one diol.
The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided.
C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
The disclosure relates to a magnetic separation rack for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) and a foot portion (8). The body portion comprises an array of sample vessel retaining portions (2) and plurality of magnetising portions (3). Each sample vessel retaining portion comprises at least one visible portion such that when a sample vessel is mounted in a sample vessel retaining portion at least one portion of the sample vessel is visible to a user. The magnetising portions are arranged within the body portion (1) such that at least two magnetising portions (3) are circumferentially spaced about each sample vessel retaining portion (2). The foot portion is pivotally coupled to the body portion such that the body portion is operatively tiltable with respect to the foot portion such that each sample vessel retaining portion may retain a sample vessel mounted therein in a tilted position with respect to the vertical. The disclosure further relates to a method of isolating magnetically labelled particles from a non-magnetic medium using the said magnetic separation rack.
The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3). The disclosure further relates to a kit of parts and a method for isolating magnetically labelled particles from a non-magnetic medium using the magnetic separation device.
The present disclosure relates to a magnetising portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetising portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetising portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.
Methods of reversal of the binding between a biotin compound and a biotin- binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. The strong interaction between streptavidin or avidin-biotin is made much weaker by using a combination of modified streptavidin or avidin and modified biotin like desthiobiotin or a derivative thereof like DSB-X Biotin. A protein, such as an antibody may be biotinylated with the modified biotin. When this protein is isolated by binding the modified biotin to the modified streptavidin or avidin bound to an solid surface, it may be released under very gently and very rapid conditions by addition of free biotin. In contrast to proteins obtained by the prior art release methods the protein obtained using the previously available release methods, the proteins obtained using the methods disclosed herein will maintain their native conformation. Uses of the methods in various procedures including cell detachment procedures and techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also described.
C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C07K 14/465 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from birds
C07K 14/36 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from ActinomycesPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Streptomyces (G)
82.
PARTICLES AND THEIR USE IN A METHOD FOR ISOLATING NUCLEIC ACID OR A METHOD FOR ISOLATING PHOSPHOPROTEINS
Monodisperse polymer microparticles comprising polystyrene or polyacrylate, wherein said particles have a coating formed of at least one transition metal oxide or porous polymer microparticles comprising polystyrene or polyacrylate, wherein said particles have a coating formed of at least one transition metal oxide. The use of such particles in a method for isolation of phosphoproteins from a sample containing phosphoproteins or for isolating nucleic acid from a sample containing nucleic acid is also described
C08F 257/02 - Macromolecular compounds obtained by polymerising monomers on to polymers of aromatic monomers as defined in group on to polymers of styrene or alkyl-substituted styrenes
C08F 265/04 - Macromolecular compounds obtained by polymerising monomers on to polymers of unsaturated monocarboxylic acids or derivatives thereof as defined in group on to polymers of esters
Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B 40/14 - Libraries containing macromolecular compounds and not covered by groups
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
C08F 287/00 - Macromolecular compounds obtained by polymerising monomers on to block polymers
C08L 53/00 - Compositions of block copolymers containing at least one sequence of a polymer obtained by reactions only involving carbon-to-carbon unsaturated bondsCompositions of derivatives of such polymers
C09D 153/00 - Coating compositions based on block copolymers containing at least one sequence of a polymer obtained by reactions only involving carbon-to-carbon unsaturated bondsCoating compositions based on derivatives of such polymers
Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.
C08F 293/00 - Macromolecular compounds obtained by polymerisation on to a macromolecule having groups capable of inducing the formation of new polymer chains bound exclusively at one or both ends of the starting macromolecule
85.
METHODS AND MATERIALS FOR THE GENERATION OF REGULATORY T CELLS
THE NORWEGIAN RADIUM HOSPITAL RESEARCH FOUNDATION (Norway)
Inventor
Aarvak, Tanja
Rasmussen, Anne Marie
Kvalheim, Gunnar
Piedras, Walter Gabriell Borelli
Brunsvig, Anne
Abstract
Methods are disclosed for the generation of immunosuppressive regulatory T cells. The methods can include contacting a population of CD4+CD25- T cells with a T cell receptor (TCR)/CD3 activator, a TCR co-stimulator activator, and rapamycin. Kits for the generation of immunosuppressive regulatory T cells, methods of use, and cell populations are also disclosed.
A magnetic separator unit for the selective separation of at least one component from a fluid mixture is described. The unit can include at least one mandrel-shaped magnet unit and tubing wrapped around it. Fluid containing magnetisable particles can be passed through the tubing to allow attraction of the particles to the magnet unit.
G01N 33/537 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
C02F 1/48 - Treatment of water, waste water, or sewage with magnetic or electric fields
B01D 35/06 - Filters making use of electricity or magnetism
B03C 1/02 - Magnetic separation acting directly on the substance being separated
87.
Process for the preparation of coated polymer particles
A process for the preparation of coated polymer particles containing superparamagnetic crystals, said process comprising reacting porous, surface-functionalized, superparamagnetic crystal-containing polymer particles of diameter 0.5 to 1.8 μm with at least one polyisocyanate and at least one diol or at least one epoxide.
A61B 5/055 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fieldsMeasuring using microwaves or radio waves involving electronic [EMR] or nuclear [NMR] magnetic resonance, e.g. magnetic resonance imaging
C12N 11/08 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
B05D 7/00 - Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials
B05D 3/02 - Pretreatment of surfaces to which liquids or other fluent materials are to be appliedAfter-treatment of applied coatings, e.g. intermediate treating of an applied coating preparatory to subsequent applications of liquids or other fluent materials by baking
B32B 19/00 - Layered products essentially comprising natural mineral fibres or particles, e.g. asbestos, mica
B32B 5/16 - Layered products characterised by the non-homogeneity or physical structure of a layer characterised by features of a layer formed of particles, e.g. chips, chopped fibres, powder
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
(1) Diagnostic and analytical preparations for use in medicine and science; chemical and biochemical reagents, substances and preparations for medical and bioscientific use, laboratory analysis, molecular biology and for other biosciences, all for research purposes; diagnostic and analytical preparations for medical purposes; diagnostic and analytical preparations for use with assay kits; assay kits for diagnostic and analytical purposes; assay kits for diagnostic and analytical purposes incorporating glass chips; assay kits for the scientific purposes; assay kits for scientific purposes incorporating glass chips; parts and fittings for the aforesaid; assay kits for medical purposes; assay kits for medical purposes incorporating glass chips; parts and fittings for the aforesaid.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
Diagnostic and analytical preparations for use in science; diagnostic and analytical preparations comprising magnetic or non-magnetic microparticles for use science; chemical products for scientific, analytical, diagnostic, pharmaceutical, medical, veterinary and hygienic purposes; chemical and biochemical reagents, substances and preparations for medical and bioscientific use, laboratory analysis, molecular biology and for other biosciences, all for research purposes; chemical and biochemical reagents, substances and preparations comprising magnetic or non-magnetic microparticles for medical and bioscientific use, laboratory analysis, molecular biology and for other biosciences, all for research purposes; preparations of polymer particles for use in medicine and science. Diagnostic and analytical preparations for use in medicine; diagnostic and analytical preparations comprising magnetic or non-magnetic microparticles for use in medicine; diagnostic preparations for medical purposes; diagnostic preparations for medical purposes for in vitro application to living matter and cells; therapeutic compositions for in vitro application to living matter and cells; diagnostic preparations for medical purposes comprising magnetic or non- magnetic microparticles for in-vitro application to living matter and cells; therapeutic compositions comprising magnetic or non-magnetic microparticles for in-vitro application to living matter and cells; preparations of polymer particles for diagnostic, pharmaceutical, medical, veterinary or hygienic purposes.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
09 - Scientific and electric apparatus and instruments
Goods & Services
Diagnostic and analytical preparations for use in medicine and science; chemical and biochemical reagents, substances and preparations for medical and bioscientific use, laboratory analysis, molecular biology and for other biosciences, all for research purposes. Diagnostic preparations for medical purposes; therapeutic compositions for in vitro application to living matter and cells. Magnetic particle concentrators to be used with magnetic particle based biomedical separations; parts and fittings for all the aforesaid goods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
chemical reagents for use in laboratory analyses and cell preparation; diagnostic preparations for scientific use; chemical preparations containing beads for analytical use in scientific laboratories; immunoglobulin preparations for analytical use in scientific laboratories; all for sale separately or in kit form [ chemical reagents for use in medical laboratory analyses, cell preparation, and in vitro testing; diagostic preparations for medical use; chemical preparations containing beads for diagnotic use in medical laboratories; diagnotic preparation; namely, immunoglobulin preparations for medical use ]
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
(1) Chemical reagents; chemical preparations containing beads and for use in laboratory analyses, cell preparation; immunoglobulin preparations; all for sale separately or in kit form.
(2) Chemical preparations containing beads for use in in vitro medical testing and diagnosis; all for sale separately or in kit form.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
Goods & Services
MICROSPHERES MADE FROM PLASTICS, NAMELY MAGNETIZABLE POLYMER BEADS FOR USE IN MEDICAL AND SCIENTIFIC RESEARCH MICROSPHERES MADE FROM PLASTIC, namely, MAGNETIZABLE POLYMER BEADS FOR THERAPEUTIC USE, AS WELL AS FOR DIAGNOSIS FOR MEDICAL PURPOSES FOR IN VITRO USE
