Abstract

An atomic sensing method, the method including providing a polarization converter; emitting a linearly polarized polychromatic laser beam to the polarization converter; converting, by the polarization converter, the linearly polarized polychromatic laser beam into a circularly-polarized laser beam and a linearly-polarized laser beam; combining the circularly-polarized laser beam and the linearly-polarized laser beam thereby yielding a multi-polarization polychromatic laser beam; transmitting the multi-polarization polychromatic laser beam to an atomic vapor cell comprising alkali metal atoms, polarizing the multi-polarization polychromatic laser beam into two laser beams, and detecting the two laser beams by two photodetectors, respectively.

IPC Classes  ?

• G01J 4/00 - Measuring polarisation of light
• G01J 4/04 - Polarimeters using electric detection means
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising
• G01R 33/032 - Measuring direction or magnitude of magnetic fields or magnetic flux using magneto-optic devices, e.g. Faraday

Abstract

Provided are a method and a system for preparing a recombinant adeno-associated virus (rAAV), and a recombinant bacmid. The method comprises: (1) respectively preparing a shuttle plasmid and a corresponding recombinant bacmid containing baculovirus genome; (2) integrating a rAAV core expression element carrying a heterologous functional gene segment with other expression cassettes of functional protein components necessary for rAAV production to obtain a recombinant bacmid containing the recombinant baculovirus genomes that produce rAAV; and (3) transfecting the recombinant bacmid into a host cell line for cultivation. The system comprises a shuttle plasmid and a corresponding recombinant bacmid containing baculovirus genome. The recombinant bacmid comprises at least one expression cassette for a functional protein component required to produce rAAV.

IPC Classes  ?

• C12N 15/866 - Baculoviral vectors
• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material

Abstract

A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.

IPC Classes  ?

• C12N 15/864 - Parvoviral vectors
• C12N 15/866 - Baculoviral vectors
• C12N 15/35 - Parvoviridae, e.g. feline panleukopenia virus, human parvovirus
• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
• C12N 15/86 - Viral vectors

Abstract

” to provide magnetic fields for the two capillary atomic cells working in the same temperature environment; a polarizing plane changes after interaction between a weak signal light and atoms.

IPC Classes  ?

• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising
• G02F 1/09 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the intensity, phase, polarisation or colour based on magneto-optical elements, e.g. exhibiting Faraday effect
• G02F 3/00 - Optical logic elementsOptical bistable devices
• G02B 27/30 - Collimators
• H04B 10/70 - Photonic quantum communication

Abstract

An inert gas nucleus channel device comprising a magnetic resonance control system, a radio frequency generator, an RF amplifier and AD converter, a frequency synthesizer, a first mixer, a second power amplifier, a second T/R switch, an inert gas nucleus coil, a second pre-amplifier and a second mixer. A magnetic resonance imaging method is also disclosed. The method controls an increase or decrease in a frequency of a pulse or echo signal excited by a radio-frequency signal, implementing magnetic resonance imaging using hyperpolarized inert gases (such as xenon, helium, and krypton), making it possible to apply MRI to lungs and brains using the hyperpolarized inert gases, and expanding the probing range of MRI. The inert gas nucleus channel device is simple in structure and easy to operate and upgrades a hydrogen nucleus magnetic resonance imaging instrument to become a multi-nuclei MRI system using the hyperpolarized inert gases.

IPC Classes  ?

• G01R 33/54 - Signal processing systems, e.g. using pulse sequences
• G01R 33/56 - Image enhancement or correction, e.g. subtraction or averaging techniques
• G01R 33/36 - Electrical details, e.g. matching or coupling of the coil to the receiver
• G01R 33/28 - Details of apparatus provided for in groups
• G01R 33/385 - Systems for generation, homogenisation or stabilisation of the main or gradient magnetic field using gradient magnetic field coils

Abstract

Disclosed is an optical-fibre atomic light-filtering apparatus comprising an optical-fibre coupling focusing collimating mirror (1), a first polarizing optical fibre (2), a first permanent magnetic ring (3), a pure iron frame shaped like the Chinese character "曰", a heat preservation box (5), a first capillary atomic cell (6), an armoured twisted-pair heating wire (7), a second permanent magnetic ring (8), a second polarizing optical fibre (9), a thermostat (10), a cable (11), a third permanent magnetic ring (12), a temperature sensor (13), a second capillary atomic cell (14), a fourth permanent magnetic ring (15), a third polarizing optical fibre (16) and a photoelectric detector (17). The two pairs of permanent magnetic rings (3, 8, 12, 15) are matched with the pure iron frame (4) shaped like the Chinese character "曰" so as to provide magnetic fields for the two capillary atomic cells (6, 14) working in the same temperature environment; a polarizing plane changes after interaction between a weak signal light (L2) and atoms; and meanwhile, the polarizing weak light signals (L2) are selected at a low attenuation rate and backlight (L1) noise is eliminated at a high extinction ratio by means of the three polarizing optical fibres (2, 9, 16). The apparatus is simple in structure, easy to operate, integrated and miniaturized, and is applicable to the extraction and measurement of the weak signal light (L2) in a free space.

IPC Classes  ?

• G02F 1/09 - Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulatingNon-linear optics for the control of the intensity, phase, polarisation or colour based on magneto-optical elements, e.g. exhibiting Faraday effect
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising

Abstract

An electron-nuclear double resonance resonator, having a loop-gap resonator and an elongated lead; the loop-gap resonator comprises a plurality of arc-shaped conductive plates, and the elongated lead connects the arc-shaped conductive plates into a radio-frequency coil; the loop-gap resonator resonates at an electron resonance frequency, and the radio-frequency coil resonates at a nuclear resonance frequency; with the structure of the loop-gap resonator, the separation between an electric field and a magnetic field can be accelerated to ensure the maximization of the ratio of the magnetic field to the electric field inside a resonant resonator; and with the elongated lead, the impact of the lead to a resonance frequency and the mode of the loop-gap resonator is prevented as much as possible, and meanwhile the conductive plates of the loop-gap resonator can be connected into the radio-frequency coil.

IPC Classes  ?

• H01P 7/00 - Resonators of the waveguide type
• G01R 33/343 - Constructional details, e.g. resonators of slotted-tube or loop-gap type
• G01R 33/34 - Constructional details, e.g. resonators
• G01R 33/60 - Arrangements or instruments for measuring magnetic variables involving magnetic resonance using electron paramagnetic resonance
• G01R 33/62 - Arrangements or instruments for measuring magnetic variables involving magnetic resonance using double resonance
• G01R 33/36 - Electrical details, e.g. matching or coupling of the coil to the receiver
• G01R 33/28 - Details of apparatus provided for in groups

Abstract

A magnetic resonance imaging device having an inert gas nucleus channel and a magnetic resonance imaging method. The device comprises a magnetic resonance imaging system (1), a radio frequency generator (2), a first power amplifier (3), a first T/R switch (4), a hydrogen nucleus coil (5), a first pre-amplifier (6), a RF amplifier and AD converter (7), a frequency synthesizer (8), a first mixer (9), a second power amplifier (10), a second T/R switch (11), an inert gas nucleus coil (12), a second pre-amplifier (13), and a second mixer (14). The method controls an increase or decrease in a frequency of a pulse or echo signal excited by a radio frequency signal, implementing magnetic resonance imaging using hyperpolarized inert gases (such as xenon, helium, and krypton), making it possible to apply MRI to lungs and brains using the hyperpolarized inert gases, expanding the probing range of MRI, and upgrading hydrogen nucleus MRI equipment to become a multi-nuclei MRI system using the hyperpolarized inert gases.

IPC Classes  ?

• G01R 33/32 - Excitation or detection systems, e.g. using radiofrequency signals

Abstract

Disclosed is a method for preparing a recombinant adeno-associated virus (rAAV). The preparation method comprises the following steps: (1) constructing a gene of interest into a recombinant baculovirus having integrated into a genome thereof elements necessary for preparing the rAAV, comprising a rep gene and a cap gene of an adeno-associated virus and an ITR core expression element carrying the gene of interest; (2) infecting a host insect larva with the recombinant baculovirus obtained in step (1) to allow the host insect larva to produce a large amount of the rAAV in vivo; and (3) lysing the host insect larva obtained in step (2) and extracting and purifying the rAAV.

IPC Classes  ?

• C12N 15/866 - Baculoviral vectors
• C12R 1/93 - Animal viruses

Abstract

A method of preparing a recombinant adeno-associated virus and a recombinant bacilliform virus are provided, and the method comprises the following steps: (1) infecting a host packaging cell line with a corresponding recombinant bacilliform virus constructed with an ITR core expression component and a Cap gene or Rep gene of a recombinant adeno-associated virus; (2) performing amplification culture on the host packaging cell line infected with the recombinant bacilliform virus, so as to produce a recombinant adeno-associated virus; and (3) separating and purifying the recombinant adeno-associated virus obtained in step (2).

IPC Classes  ?

• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
• C12N 15/866 - Baculoviral vectors

Abstract

Provided are a recombinant baculovirus and application thereof. The recombinant baculovirus integrates Rep genes, Cap genes and ITR core expression elements of adeno-associated viruses, and is applicable to preparation of recombinant adeno-associated virus vectors for gene therapy.

IPC Classes  ?

• C12N 15/86 - Viral vectors
• C12N 15/866 - Baculoviral vectors
• C12N 15/33 - Genes encoding viral proteins
• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12R 1/93 - Animal viruses

Abstract

An electronic-nuclear double-resonance resonator comprises a fracture cavity and elongate wire (102), wherein the fracture cavity is composed of a plurality of arc-shaped guide plates (101a, 101b, 101c, 101d), and the arc-shaped conductors (101a, 101b, 101c, 101d) are connected into a radio frequency coil by means of the elongate wire (102); the fracture cavity is resonant at an electronic resonant frequency, and the radio frequency coil is resonant at an atomic nucleus resonant frequency; using a fracture cavity structure can prompt the separation of an electromagnetic field and ensure the maximization of a specific value of an internal magnetic field of a resonant cavity and an electric field; and a resonant frequency and mode of the fracture cavity will not be influenced as far as possible by using the elongate wire (102), while the conductors of the fracture cavity can be connected into the radio frequency coil. In the electronic-nuclear double-resonance resonator, the fracture cavity and the radio frequency coil are ingeniously combined into one piece by means of the elongate wire (102), so that the efficiency of two resonant modes is ensured, and the structure of the electronic-nuclear double-resonance resonator is simplified at the same time.

IPC Classes  ?

• G01R 33/343 - Constructional details, e.g. resonators of slotted-tube or loop-gap type

Abstract

A physical unit of a chip-scale nuclear magnetic resonance (NMR) gyroscope, the physical unit including: a vertical cavity surface emitting laser (VCSEL), a silicon sheet including a recess, a glass sheet, an atomic vapor chamber, a first right angle prism, a quarter-wave plate, a polarizing beam splitter, and photodetectors. The recess includes sides including reflecting mirrors. The glass sheet is disposed on the silicon sheet. The recess of the silicon sheet is in a structure of an inverted square frustum, and the reflecting mirrors are disposed on sides of the recess. The atomic vapor chamber is an enclosed region formed between the recess and the glass sheet. The atomic vapor chamber is filled with alkali metal atoms, one or a plurality of inert gas atoms, and one or a plurality of buffer gases.

IPC Classes  ?

• G01C 19/62 - Electronic or nuclear magnetic resonance gyrometers with optical pumping

Abstract

Disclosed in the present invention are a combined preparation and use thereof for preparing a non-small cell lung cancer drug, wherein a EGFR tyrosine kinase inhibitor and a preparation for enhancing the concentration of glutathione (GSH) in lung cancer cells are administrated simultaneously or sequentially. The present invention has found, through systematic study, that GSH plays an important role in the tolerance to drugs such as EGFR tyrosine kinase inhibitors in non-small cell lung cancers with EGFR T790M mutation, and then uses a method for improving the GSH concentration within lung cells to make resistant cells resensitized to the treatment of drugs such as EGFR tyrosine kinase inhibitors. Cell experiments and animal experiments have both proved that the method is safe and effective and can effectively kill cancer cells and inhibit the proliferation of cancer cells.

IPC Classes  ?

• A61K 45/06 - Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• A61K 31/192 - Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid
• A61K 31/194 - Carboxylic acids, e.g. valproic acid having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
• A61P 35/00 - Antineoplastic agents
• A61P 11/00 - Drugs for disorders of the respiratory system

Abstract

A device for producing a coherent bi-color light source, including: an array substrate, a first laser tube driven by a first direct current signal, a second laser tube driven by a modulation signal coupled by a microwave signal and a second DC signal, a half wave plate, a birefringent crystal, a first quarter wave plate, a partially reflecting plane mirror, and a second quarter wave plate. The first laser tube and the second laser tube are fixed on the array substrate. The half wave plate, the birefringent crystal, the first quarter wave plate, the partially reflecting plane mirror, and the second quarter wave plate are disposed in sequence in an emission direction of a laser beam emitted by the first laser tube. The second laser tube is disposed opposite to the birefringent crystal.

IPC Classes  ?

• H01S 3/08 - Construction or shape of optical resonators or components thereof
• H01S 3/106 - Controlling the intensity, frequency, phase, polarisation or direction of the emitted radiation, e.g. switching, gating, modulating or demodulating by controlling devices placed within the cavity
• H01S 3/10 - Controlling the intensity, frequency, phase, polarisation or direction of the emitted radiation, e.g. switching, gating, modulating or demodulating

Abstract

A physical system for a chip-scale coherent population trapping (CPT) atomic clock. The physical system includes: a vertical-cavity surface-emitting laser (VCSEL) device, a first polarizing beam splitter, a first λ/4 wave plate, a chip of an atomic vapor cell, a second λ/4 wave plate, a reflection device, a lens, a second polarizing beam splitter, and a photo detector. The first polarizing beam splitter, the first λ/4 wave plate, the chip of the atomic vapor cell, the second λ/4 wave plate, and the reflection device are disposed in sequence. The lens, the second polarizing beam splitter, and the photo detector are disposed in sequence.

IPC Classes  ?

• G04F 5/14 - Apparatus for producing preselected time intervals for use as timing standards using atomic clocks
• H03L 7/26 - Automatic control of frequency or phaseSynchronisation using energy levels of molecules, atoms, or subatomic particles as a frequency reference
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising
• H01S 1/06 - Masers, i.e. devices using stimulated emission of electromagnetic radiation in the microwave range gaseous

Abstract

A compact dual-channel atomic light filter, consisting of a light shielding box (101), a magnetic shielding box (102), two atomic foam chambers (203, 303), two magnets (103, 104), two temperature controlled furnaces (204, 304), a two-way temperature controller (105), four polarization beam splitters (201, 206, 301, 306), and four polarizers (202, 205, 302, 305). Using the organic integration of a dual-clear-aperture magnet, the two-way temperature controller and the polarization beam splitters, the present invention integrates two sets of independent atomic light filters into a compact dual-channel atomic light filter, improves the stability and consistency of the dual-channel atomic light filter, reduces the size, weight and power consumption of the whole system, and provides an effective means for the long-term stable operation of the atomic light filter.

IPC Classes  ?

• G02B 5/20 - Filters
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising

Abstract

A coherent bi-color light source device comprises an array substrate (10), a first laser tube (11) driven by a direct current signal (I1) and a second laser tube (12) driven by a modulation signal (I2) obtained by coupling a microwave signal and a direct current signal. The first laser tube (11) and the second laser tube (12) are fixed on the array substrate (10). A half-wave plate (13), a birefringent crystal (14), a first quarter-wave plate (15), a plane reflector (16) and a second quarter-wave plate (17) are arranged in sequence along an emergence direction of a laser beam emitted by the first laser tube (11). The second laser tube is opposite to the birefringent crystal (14). The coherent bi-color light source device can generate a coherent bi-color light source with linear polarization directions vertical to each other and having high purity, thereby improving quality of a coherent population trapping signal, and further improving stability of an output frequency of a coherent population trapping atomic clock. Also, the coherent bi-color light source device has a simple structure, small volume and low manufacturing cost. A coherent bi-color light generation method is further provided.

IPC Classes  ?

• G04F 5/14 - Apparatus for producing preselected time intervals for use as timing standards using atomic clocks
• H03L 7/26 - Automatic control of frequency or phaseSynchronisation using energy levels of molecules, atoms, or subatomic particles as a frequency reference
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising

Abstract

A physical system device for a chip CPT atomic clock; linearly polarized circular divergent light (12a) emitted by a vertical-cavity surface-emitting laser (VCSEL) device sequentially passes through a first polarization beam splitter (6a), a first λ/4 wave plate (7a), an atomic vapor cavity chip (8) and a second λ/4 wave plate (7b), is reflected by a reflecting device to pass through a lens (10), and is then reflected by a second polarization beam splitter (6b) to sequentially pass through the first λ/4 wave plate (7a), the atomic vapor cavity chip (8) and the second λ/4 wave plate (7b), and is then reflected by the reflecting device to sequentially pass through the lens (10) and the second polarization beam splitter (6b), and is transmitted to a photoelectric detector (11) and converted into a current for output. The device can concentrate atoms at "0-0 energy level", thus enhancing a CPT resonance signal, and improving the signal-to-noise ratio and contrast of the signal; and moreover, integrating a light source and a detector on the same silicon wafer, thus miniaturizing the device.

IPC Classes  ?

• G04F 5/14 - Apparatus for producing preselected time intervals for use as timing standards using atomic clocks
• H03L 7/26 - Automatic control of frequency or phaseSynchronisation using energy levels of molecules, atoms, or subatomic particles as a frequency reference
• G02B 27/28 - Optical systems or apparatus not provided for by any of the groups , for polarising

Abstract

A method and a device for implementing a Ramsey-CPT atomic clock by microwave On-Off modulation VCSEL are disclosed. The method includes the following steps: A, coupling direct current and microwave to a laser tube, scanning the direct current to obtain a plurality of absorption peaks and locking the direct current; B, switching on and off the microwave to realize equivalent laser pulse, and scanning the microwave to obtain Ramsey-CPT stripes; and C, locking the microwave to obtain stable frequency output. The device is characterized in that: a current source is connected with a Bias-Tee, a microwave source is connected with the Bias-Tee through a microwave switch, the Bias-Tee is connected to a laser generating device, the output laser passes through a physical system and then enters a laser detection device, and control equipment is connected with the current source, the microwave source, the microwave switch and the laser detection device respectively. The method and the device enable laser-atom periodic interaction through microwave On-Off, thereby providing a more predominant frequency discrimination curve compared with the traditional CPT scheme, and implementing more stable atomic clock. In addition, compared with the Ramsey-CPT atomic clock by using the AOM with larger volume and higher power consumption, the device has a simple structure, adopts a unique method, is easy for microminiaturization, and overcomes the key technology of the chip Ramsey-CPT atomic clock.

IPC Classes  ?

• H03L 7/26 - Automatic control of frequency or phaseSynchronisation using energy levels of molecules, atoms, or subatomic particles as a frequency reference

Abstract

−5 can be obtained, error rate generated by background light noise can be reduced by magnitude order of 2-3. Further, the distance for carrying out free-space quantum communication can be increased. Moreover, the free-space quantum cryptography communication device can normally operate in a good manner under background light noise with sunlight or moonlight.

IPC Classes  ?

• H04K 1/00 - Secret communication