Provided in the present invention is a method for modifying a solid-phase support. The solid-phase support is suitable for adhering to cells. The method comprises: bringing a solid-phase support into contact with a polylysine–protein factor mixed solution, wherein the polylysine–protein factor mixed solution comprises polylysine and a protein factor, and the protein factor comprises at least one of an extracellular matrix protein and a cellular integrin. In the method, by modifying the solid-phase support, a large number of cells can be captured by the solid-phase support, and the cells captured by the solid-phase support have clear outlines, such that the cell capture efficiency of the solid-phase support is improved.
Provided are a fluid path and a use method therefor, a fluid path system and a use method therefor, and a sequencer. The fluid path comprises a first surface and a second surface; the first surface at least comprises an exposed chip surface; the first surface and the second surface are arranged at a preset distance; a preset position of the second surface is provided with a reagent fluid; and the second surface can move in a preset direction, and drive the reagent fluid at the preset position to be at least injected into a gap between the chip surface and the second surface. Movement of the second surface drives the reagent fluid to move, thus avoiding using pressure to drive the reagent fluid; the chip surface is in a static state, such that the average flow rate of the fluid path is half of the movement speed of the second surface, and the thickness of the fluid entering the gap will be at least twice the original thickness, thereby reducing the reagent thickness of fluid intake; and the fluid path is an open fluid path, and the first surface and the second surface do not need to be fixed by adhesive bonding, thus overcoming the technical challenge of sealing fluid paths under the condition of small gaps.
B05C 9/00 - Apparatus or plant for applying liquid or other fluent material to surfaces by means not covered by groups , or in which the means of applying the liquid or other fluent material is not important
3.
DUAL-LUCIFERASE REPORTER GENE DETECTION SYSTEM, FLUORESCENCE DETECTION KIT, AND METHOD FOR DETECTING LUMINESCENT SIGNAL OF LUCIFERASE
Provided are a dual-luciferase reporter gene detection system and use thereof. The detection system includes Gaussia luciferase, Pleuromamma xiphias luciferase, and a substrate. The substrate is coelenterazine or a derivative thereof. Compared with other dual-luciferase reporter gene detection systems, the present dual-luciferase reporter gene detection system produces stronger signals and requires simple reaction conditions.
Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).
Provided are a method for performing position labeling of nucleic acid molecules, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.
Provided are a construction method and a sequencing method for a single-cell transcriptome sequencing library and a test kit for preparing a single-cell transcriptome library. In the method, a single-cell suspension is subjected to in-situ reverse transcription in a cell or a cell nucleus, the cell or the cell nucleus, together with a magnetic bead, is overloaded into a droplet for a droplet PCR reaction, and then the magnetic bead is recovered and subjected to secondary amplification, fragmentation and library construction, such that the single-cell transcriptome sequencing library is obtained. According to the method, overloading of the cell or cell nucleus subjected to the in-situ reverse transcription together with the pretreated magnetic bead is achieved, an ultra-high throughput of 100,000 cells in a single experiment is obtained, and quite high accuracy is exhibited.
A method for sequencing polynucleotides by using nanopores. The present invention relates to, in particular, a method for sequencing polynucleotides by means of covalently binding a polynucleotide binding protein and a nanopore.
The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same. The nucleic acid molecule contains at the end a modified nucleotide capable of blocking a motor protein, and the modified nucleotide is as shown in Formula I or Formula I′:
The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same. The nucleic acid molecule contains at the end a modified nucleotide capable of blocking a motor protein, and the modified nucleotide is as shown in Formula I or Formula I′:
The present application relates to transcriptome sequencing and biomolecule space information detection. Specifically, the present application relates to a method for positioning and labeling a nucleic acid molecule, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.
Provided in the present disclosure is a method for sequencing a double-stranded target polynucleotide. The method can keep ATP at a relatively constant concentration during sequencing, so that the sequencing rate can be better kept stable and unchanged. Further provided in the present disclosure is a kit for sequencing a double-stranded target polynucleotide, the kit including a transmembrane pore in the membrane, a helicase, an ATP-generating enzyme and an ATP-generating substrate.
C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
C12Q 1/50 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase involving creatine phosphokinase
11.
MEMBRANE FORMING DEVICE AND MEMBRANE FORMING METHOD
A membrane forming device and a membrane forming method are provided. The membrane forming device includes: a base (10); a main body (20) arranged to the base (10) and the main body (20) having a recess; and an opening member (30) arranged inside a recess port on an end of the recess away from the base (10), and a hollow portion (32) of the opening member (30) being configured to form a amphipathic molecular membrane (80), wherein a cross-sectional area of the hollow portion (32) of the opening member (30) is smaller than that of the accommodating chamber (21).
Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.
The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.
C12N 15/70 - Vectors or expression systems specially adapted for E. coli
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
A mutant and the uses thereof. Compared with the amino acid sequence shown in SEQ ID NO: 2, the mutant has at least one of the following mutation sites: the 24th, 26th, 27th, 29th, 30th, 31st, 32nd, 33rd, 36th, 37th, 40th, 66th, 79th, 84th, 88th, 102nd, 103rd, 104th, 110th, 123rd, 124th, 138th, 152nd, 163rd, 167th, 170th, 174th, 175th, 178th, 182nd and 183rd sites.
C12Q 1/66 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving luciferase
15.
Method for Improving Sequencing Resolution, and Sequencing Apparatus and System
Disclosed are a method for increasing a sequencing resolution, and a sequencing device and system. The method includes carrying out irradiation and excitation on a sample to be tested containing a fluorescent dye with at least one type of excitation light to generate a fluorescence signal, where a maximum wavelength in the excitation light is smaller than a wavelength of red light; and the transmitting the generated fluorescence signal through an optical path, and collecting and imaging the signal.
Provided are a method for positioning and labeling a nucleic acid molecule, and a method for constructing a nucleic acid molecule library for single-cell transcriptome sequencing, which relate to the technical fields of single-cell transcriptome sequencing and biomolecular space information detection. Further provided are a nucleic acid molecule library constructed by using the method, and a kit for implementing the method.
The present invention relates to the field of biotechnology, in particular to a DNA polymerase mutant and the use thereof. Compared with a wild type, the mutant provided in the present invention has a significantly improved DNA polymerization activity, significantly improved affinity to DNA and amplification uniformity; and has good effects in the amplification of multiplex PCR, high-GC templates and complex templates. A hot-start-version DNA polymerase is further prepared by means of using antibodies or chemical modifications, wherein the yield of the DNA polymerase is significantly improved, while the primer dimer is significantly decreased. The prepared mutant hot-start DNA polymerase can be applied to PCR amplification of multiplex PCR, high-GC templates and complex templates.
A DNA library sequence for increasing the number of chip probes and an application thereof. The DNA library sequence comprises two or more position information labeling sequences and three or more fixed sequences, wherein the position information labeling sequences and the fixed sequences are arranged at intervals. The DNA library sequence increases the number of probes of a chip used for spatiotemporal omics, and then a capture number of transcript genes is increased. The present invention has a very high application prospect.
Described herein are high coverage single tube Long Fragment Read (stLFR) technology which uses performs stLFR on target DNA fragments that have already been amplified before they are co-barcoded, which provides higher amount of DNA for sequencing and increases sequencing coverage. In some embodiments, the high coverage stLFR described in this application uses two rounds of stLFR. In some embodiments, the target DNA fragments are transposed with transposons having particular positional barcodes that can be used to order sequence reads.
The present application provides a detection chip assembly tool, a liquid injection apparatus and method, an electronic device, and a medium. The tool is mounted on a vacuumizing assembly, and comprises: a base mounted on a detection chip. The base is provided with a reaction cavity covering the detection chip and a fluid channel communicated with the reaction cavity, and is further provided with a liquid inlet groove structure communicated with the fluid channel and having a buffer solution injected. When the detection chip is filled with the buffer solution, the vacuumizing assembly is started to discharge air in the detection chip assembly tool, in response to the vacuumizing assembly vacuumizing, pressure relief is performed, and the buffer solution in the liquid inlet groove structure flows into the reaction cavity along the fluid channel under the action of negative pressure so as to fill the detection chip.
A microfluidic device and a microfluid detection device, comprising a body (100) and a liquid channel (201) provided within the body (100); a fluid input port (103) communicated with the liquid channel (201) is also provided on the body (100), and an arrangement position of the fluid input port (103) does not include a sensing area (301). The microfluidic device and the microfluidic detection device are easy to operate, and can effectively solve the problem of introducing bubbles due to directly opening a liquid injection hole on the sensing area (301).
A method for generating a DNA storage encoding/decoding rule, and a method for DNA storage encoding/decoding. The method for generating the DNA storage encoding/decoding rule includes: setting a sliding window for the DNA storage encoding/decoding rule; screening out, from a full set of sequences, a set of qualified sequences complying with a limiting condition; connecting the sequences in the set of qualified sequences by means of a directed graph; deleting, in the directed graph, nodes of which the number of out-degree is less than a set threshold for the number of out-degree; deleting excess out-degree of each node in the directed graph; and acquiring an algorithm chart, which includes the DNA storage encoding/decoding rule.
Provided are a thermostable B-family DNA polymerase mutant and an application thereof. The thermostable B-family DNA polymerase mutant is provided to mutate amino acid residues in at least two of three sites of group A, i.e., 408, 409, and 410 in an amino acid sequence of thermostable B-family DNA polymerases to obtain proteins having DNA polymerase activity. A-group sites (408/409/410), B-group sites (451/485), C-group sites (389/383/384), D-group sites (589/67/680), E-group sites (491/493/494/497), and F-group sites (474/478/486/480/484) of several B-family DNA polymerases are analyzed to design the mutant.
Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.
This application provides a microwell structure, a preparation method and a chip, which belongs to the field of biotechnology. The microwell structure provided in this application comprises a base and support structure layers having through holes and being provided over the base; the support structure layers and the through holes form a cavity with the base; from top to bottom, the support structure layers comprise at least two layers, and the diameter of the through hole in the support structure layer far away from the base is smaller than that of the through hole in the support structure layer provided over the base. With this structure, since the diameter of the through hole in the support structure layer at the top is smaller, it can achieve a better fixing effect on the membrane, thereby increasing the stability of the membrane; the through hole in the support structure layer over the base has a relatively large diameter and communicates with the through hole with a smaller diameter in the support structure layer at the top, thereby obtaining a structure similar to a hole with a smaller opening diameter but a larger volume, which fundamentally solves the stability problem of the membrane and problems of the applicability of a common membrane, the cost and the like, caused by the developing a suitable complex artificial membrane material.
Provided are a detection structure and method, a detection chip, and a sensing device. The detection structure (200) includes a sensing device (100) at least including a detection chip (000), a fluid tank (101), and a carrier plate (102), and a detector (201) configured to capture and analyze a signal generated in the sensing device (100). The fluid tank (101) is disposed at the carrier plate (102) and forms a cavity (103) with the carrier plate (102). The detection chip (000) is located in the cavity (103) and at least includes a substrate (001), a first electrode (002) and a first circuit (003) that are disposed at the substrate. The first electrode (002) is connected to a second electrode (004) through the first circuit (003) to form an electrical circuit.
A method for determining an effect relationship between various substances and cells, including: providing a first droplet, a second droplet, a third droplet, and a microwell array chip, a plurality of microwell combinations being provided on the microwell array chip, fusing the first droplet and the second droplet on the microwell array chip, and performing cell culture; adding the third droplet into the microwell array chip, and fusing the third droplet with the first fused droplet subjected to the cell culture, to obtain a second fused droplet, performing demulsification, library construction, and sequencing, and determining an effect relationship between various substances and cells on the basis of the sequencing result.
An illumination chip includes an illumination array structure and an illumination well. The illumination array structure includes a substrate and multiple illumination units periodically distributed on the substrate. The illumination well is disposed on a surface, extending along the multiple illumination units, of the illumination array structure, where the illumination well is divided into multiple placement units which are configured to place samples, and each placement unit (841) is disposed above a corresponding illumination unit. Illumination units of the multiple illumination units are configured to generate, in a case where the illumination units of the multiple illumination units are illuminated by a light source, surface plasmon structured light to excite fluorescent dyes of samples in corresponding placement units of the multiple placement units, and generate a fluorescence signal.
Provided is a method for obtaining a double-stranded sequence by single-stranded rolling circle amplification, comprising: 1) performing rolling circle amplification reaction on single-stranded circular DNA by means of a first primer to obtain an amplified sequence, the first primer being complementary to a partial region of the single-stranded circular DNA, and the single-stranded circular DNA having a break mechanism that can cause the single-stranded circular DNA to ring-open; 2) ring-opening the single-stranded circular DNA by means of the break mechanism to obtain single-stranded linear DNA; and 3) using the single-stranded linear DNA as a second primer and using the amplified sequence obtained in step 1) as a template to perform amplification reaction to obtain an amplified double-stranded sequence.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
C12Q 1/527 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving lyase
C40B 50/06 - Biochemical methods, e.g. using enzymes or whole viable microorganisms
30.
PREPARATION OF MONODISPERSE POLYMER BEADS WITH ROOM TEMPERATURE INITIATION METHODS
Techniques are described for preparing monodisperse polymer beads, such as using monosized seed particles, where the process of growing, polymerizing, or swelling the beads during preparation occurs at low, ambient, or room temperature. A variety of schemes are disclosed for growing, polymerizing, or swelling that avoid thermal initiation-based swelling. For example, photo polymerization, metal-free atom transfer radical polymerization, and redox polymerization schemes are disclosed. Additional features may be implemented in the monodisperse polymer beads, such as controlling bead density, controlling porous character, and inclusion of various chemical functionalities, including protected functionalities that can be activated or de-protected after preparation of the monodisperse polymer beads, such as during subsequent use.
C08F 257/02 - Macromolecular compounds obtained by polymerising monomers on to polymers of aromatic monomers as defined in group on to polymers of styrene or alkyl-substituted styrenes
31.
Magnetic bead-based detection method, storage medium, and detection device
Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).
G06K 7/08 - Methods or arrangements for sensing record carriers by means detecting the change of an electrostatic or magnetic field, e.g. by detecting change of capacitance between electrodes
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
The present invention relates to the field of nucleic acid sequencing. Specifically, provided in the present invention is a nucleotide analogue for sequencing. The nucleotide analogue carries a linker on the base or the ribose ring 3′-OH, and can be used for NGS sequencing. The present invention further relates to a kit containing the nucleotide analogue, and a sequencing method based on the nucleotide analogue.
A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
34.
Method for Constructing RNA Sequencing Library, Sequencing Method and Kit
Provided in the present application is a method for constructing an RNA sequencing library, a sequencing method and a kit. The construction method comprises: acquiring a single-stranded cDNA, which is a reverse transcription product of mRNA, the 3′-terminal of the single-stranded cDNA including a cDNA tag sequence; cyclizing the single-stranded cDNA to obtain a single-stranded cyclized cDNA; amplifying the single-stranded cyclized cDNA with a primer combination, which is formed by a random primer or a gene-specific primer and a cDNA tag primer, so as to obtain an amplified fragment, the cDNA tag primer being at least a part of the cDNA tag sequence; and performing fragmentation for library construction on the amplified fragment, so as to obtain an RNA sequencing library.
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/25 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving enzymes not classifiable in groups
C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
C07K 16/44 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere
C12Q 1/6804 - Nucleic acid analysis using immunogens
Described is a free DNA-based disease prediction model and a construction method therefor and an application thereof. The construction method includes the steps of: 1) obtaining sequencing data of free DNA samples of diseased individuals and control individuals, the number of the diseased individuals and the number of the control individuals being both multiple; 2) selecting, according to the coverage of the sequencing data of the free DNA samples of the diseased individuals and the control individuals on a genome, a gene set having a difference in the coverage of a transcription initiation site region between the diseased individuals and the control individuals; and 3) for genes in the gene set, using the coverage of the sequencing data on the gene transcription initiation site region as an input prediction model for training so as to establishing a disease prediction model.
C12Q 1/6886 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
G16B 25/10 - Gene or protein expression profilingExpression-ratio estimation or normalisation
A method for analyzing cell epigenomics from multiple dimensions. The method comprises the following steps: by using ChiTag transposase and conventional Tn5 transposase in cells, respectively embedding different linker sequences, and achieving common analysis of information of a chromatin open region and information of a specific protein binding sequence on a cellular level. The method has important application prospects in aspects such as study of development and/or disease related cell population heterogeneity, drawing of a cell map, analysis of tumor cells having different clinical characteristics, and clinical study of evolution and/or metastasis of tumor cells.
The present invention relates to the field of biotechnology. A method and system of detecting fetal chromosomal abnormalities are disclosed. The method comprises: (1) obtaining sequencing data of cell-free nucleic acid fragments and clinical phenotypic feature data from a pregnant woman to be detected, wherein the sequencing data comprise a plurality of read segments, and the clinical phenotypic feature data of the pregnant woman form a phenotypic feature vector of the pregnant woman, (2) performing window division on at least part of a chromosome sequence of a reference genome to obtain sliding windows, counting the read segments falling within the sliding windows, and generating a sequence feature matrix of the chromosome sequence; (3) inputting the sequence feature matrix into a trained machine learning model to extract a sequence feature vector of the chromosome sequence; and (4) combining the sequence feature vector and the phenotypic feature vector of the pregnant woman to form a combined feature vector, and inputting the combined feature vector into a classification detection model to obtain the fetal chromosomal abnormality state of the pregnant woman to be detected.
G16B 20/20 - Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
The present disclosure relates to an image processing method, apparatus and system. The image processing method comprises: with regard to the current cycle and each of at least one reference cycle adjacent to the current cycle, acquiring a plurality of substrate images of a plurality of fluorescence channels of a sequencing substrate in each cycle, wherein the current cycle and the at least one reference cycle are a plurality of consecutive biochemical reactions and imaging cycles that occur in sequence, each fluorescence channel corresponds to one substrate image, and a pixel value of the substrate image of each fluorescence channel represents a fluorescence intensity value under each fluorescence channel; acquiring a fluorescence intensity value in a pixel neighborhood at the same position on each substrate image corresponding to each cycle; and according to fluorescence intensity values in pixel neighborhoods at the same position corresponding to the current cycle and the at least one reference cycle, identifying a base at a base position corresponding to the current cycle. By means of the present disclosure, the accuracy of image recognition can be improved.
The present disclosure relates to an optical computing method and system, and a controller and a storage medium. The optical computing system comprises: an analog-to-digital converter array, which is configured to convert into a digital signal a current that is generated when a polymer is translocated with respect to nanopores; and an optical computing chip, which is configured to identify the arrangement of polymer units in the polymer according to the digital signal. The optical computing chip which has better computing power than a GPU in the present disclosure is applied to nanopore-based polymer sequencing, thereby optimizing the computing speed of the optical computing chip in "identifying polymer units in a valid signal" when a plurality of nanopores generate data in parallel.
A microbead used in biological detection is provided. The microbead includes a first coating layer, a magnetic layer, and a second coating layer. The first coating layer includes a first top surface and a first side surface connecting the first top surface. The magnetic layer is formed on the first top surface, and the magnetic layer includes a second top surface away from the first top surface and a second side surface connecting the second top surface. The second coating layer includes a top wall and a periphery. The top wall is connected to the second top surface, and the periphery is connected to the top wall, the second side surface, and the first side surface, so the first coating layer and the second coating layer coat the magnetic layer inside the microbead. A preparation method of the microbead is also provided. The utilization rate of the microbead is improved.
Disclosed herein are methods and compositions for corresponding positions on an array with differently labeled affinity reagents immobilized at the positions. A first and a second affinity reagents are immobilized on at least some of the first and second positions, respectively. The first affinity reagent and the second affinity reagent are associated with a first luciferase polypeptide and a second luciferase polypeptide, respectively. The first luciferase polypeptide does not cross react with the second substrate and the second luciferase polypeptide does not cross react with the first substrate. The method further comprises contacting the array with the first substrate, which reacts with the first luciferase polypeptide and detecting the first luminescent signal and detecting the second luminescent signal at positions, thereby corresponding positions on an array with the first affinity reagent or the second affinity reagent immobilized at the positions.
A method for activating an adaptive immune response by adding allogeneic dendritic cells (DCs) and/or viral antigen peptides to conventional DC vaccines to expand the DC vaccine antigen spectrum with the aid of exogenous DC effect, thereby enhancing the anti-tumor effect of the DC vaccine.
Provided is a synthesis apparatus for synthesizing biomacromolecules. The synthesis apparatus includes a feeding apparatus, a recognition apparatus, a reaction apparatus, a sorting device, and a transfer device. The feeding apparatus includes a transporting assembly that can simultaneously transporting biochips. The recognition apparatus recognizes an identification of each biochip on the transporting assembly and feed back an identification information to a control device. The reaction apparatus includes various reaction vessels for performing synthesis reactions on the biochips. The sorting device includes various first sorting assemblies located on a side of the transporting assembly and correspond to the reaction vessels. The first sorting assemblies sort the chips on the transporting assembly to the reaction vessels corresponding to the current synthesis reactions when controlled by the control device. The transfer device transfers the biochips in the reaction vessels to the feeding device. Further provided are a synthesizer and a synthesis method.
A flow cell includes a first cover; a second cover facing the first cover, and at least two spacers provided side by side between the first cover and the second cover. The first cover, the second cover, and two adjacent spacers cooperatively form a flow path, surface of the first cover near the second cover piece forms a detection surface. A first opening is provided at an end of the flow path, which can inject a first sample into the flow path. The first sample is adsorbed on the detection surface. A second opening is provided at another end of the flow path, which can inject microdroplets into the flow path. The microdroplets include a second sample. A liquid inlet and outlet device applying flow cell and a sample analysis system are further provided.
Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.
b) includes an image sensor (113). The illumination light source (111) is used to emit illumination light to irradiate the microbeads (15), causing the irradiated microbeads (15) to be imaged on the image sensor. The excitation light source (112) is used to emit excitation light to excite the microbeads (15) to generate specific signals. The image sensor (113) is used to collect the images of the microbeads (15) and the specific signals to generate images. The imaging system (11) does not require a lens system. The present disclosure improves a detection efficiency of the microbeads (15).
G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
G03H 1/04 - Processes or apparatus for producing holograms
G03H 1/00 - Holographic processes or apparatus using light, infrared, or ultraviolet waves for obtaining holograms or for obtaining an image from themDetails peculiar thereto
49.
Biological sample image collection device and gene sequencer
A biological sample image collection device (100), comprising support (30) and an optical imaging assembly (50), also comprises: a plurality of movable platforms (40) for placing biological samples (20), wherein the plurality of movable platforms (40) are arranged on the support (30) in an array; the plurality of movable platforms (40) can move relative to the support (30); and forces acting on the support (30) during the movement of the movable platforms can cancel each other out, so as to avoid vibrations affecting the support (30) and the biological samples (20) are canceled. The optical imaging assembly (50) collects images of the biological samples (20) on the movable platforms (40) when the plurality of movable platforms (40) move, relative to the center of the array, in the same direction and at the same speed. Further provided is a gene sequencer including the biological sample image collection device (100).
Disclosed is an antibody capable of specifically recognizing PCSK9 or an antigen-binding fragment thereof. The antibody comprises heavy chain variable region CDR1, CDR2, and CDR3 sequences shown in SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3 or shown in amino acid sequences having at least 95% identity to SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3. The antibody can specifically bind to PCSK9 and inhibit the activity of PCSK9.
An integrated circuit for controlling a sensor chip capable of sensing various materials includes a plurality of amplifier clusters, a plurality of analog multiplexers, and at least one analog-to-digital converter coupled the analog multiplexers and configured to generate digital code values representative of electrical signals. Each of the amplifier clusters include four amplifiers, each amplifier has a first input coupled to a sensor of the sensor chip, and a second input coupled to a programmable voltage reference. Each one of the analog multiplexers is coupled to one of the amplifier clusters and configured to selectively pass through an electrical signal to the at least one analog-to-digital converter.
Provided is a sequencing chip. The sequencing chip includes: a chip main body, nucleic acids, and a phosphonic acid polymer film. The chip main body includes a plurality of chip particles arranged in a same layer, the chip particles are obtained by cutting a chip matrix along cutting lines of a wafer layer, and the chip matrix includes: the wafer layer having the cutting lines uniformly distributed thereon; a first silicon oxide layer made of silicon oxide and formed on an upper surface of the wafer layer; and a transition metal oxide layer made of a transition metal oxide and formed on an upper surface of the first silicon oxide layer. The nucleic acids are fixed on the transition metal oxide layer; and the phosphonic acid polymer film is made of a polyphosphonic acid polymer and formed on an upper surface of the transition metal oxide layer.
A method for information encoding and decoding, and method for information storage and interpretation are provided. The information encoding method includes: first binary information and second binary information as well as a first encoding rule and a second encoding rule are obtained; a first output candidate symbol corresponding to a current input of the first binary information is obtained and a second output candidate symbol corresponding to a current input of the second binary information is obtained, and an intersection of the first output candidate symbol and the second output candidate symbol is taken as an output corresponding to a current input; and an output symbol corresponding to each binary bit of the first binary information and the second binary information is sequentially determined through the first encoding rule and the second encoding rule, as to obtain an encoding sequence formed by a plurality of the output symbols.
Provided in the present disclosure are a method for determining fetal nucleic acid concentration and a fetal genotyping method. According to the embodiments of the present disclosure, the method for determining cell-free fetal nucleic acid concentration includes: (1) acquiring sequencing data of a first nucleic acid sample of a pregnant woman and a reference genome sequence, the first nucleic acid sample of the pregnant woman containing cell-free fetal nucleic acids, and the sequencing data being composed of a plurality of sequencing reads; (2) selecting a predetermined region on the reference genome sequence and determining, based on the sequencing data of the first nucleic acid sample of the pregnant woman, mutation information in the predetermined region; and (3) determining the concentration of cell-free fetal nucleic acids corresponding to the predetermined region based on the mutation information in the predetermined region.
Provided is a MART-1 (27-35) epitope-specific T cell receptor, comprising an α chain and a β chain. The α chain comprises three complementary determining regions, respective sequences thereof being positions 61-66, positions 84-89, and positions 124-136 of SEQ ID No. 3. The β chain comprises three complementary determining regions, respective amino acid sequences thereof being positions 46-50, positions 68-73, and positions 112-125 of SEQ ID No. 4. A T cell expressing the TCR can effectively recognize a MART-1 (27-35) epitope polypeptide supported on a T2 cell and secrete IFN-γ, thereby demonstrating the functionality of the receptor. Use of the TCR with a relevant drug target allows for effective drug development.
Techniques are described for preparing monodisperse polymer beads, such as using monosized seed particles, where the process of growing, polymerizing, or swelling the beads during preparation occurs at low, ambient, or room temperature. A variety of schemes are disclosed for growing, polymerizing, or swelling that avoid thermal initiation-based swelling. For example, photo polymerization, metal-free atom transfer radical polymerization, and redox polymerization schemes are disclosed. Additional features may be implemented in the monodisperse polymer beads, such as controlling bead density, controlling porous character, and inclusion of various chemical functionalities, including protected functionalities that can be activated or de-protected after preparation of the monodisperse polymer beads, such as during subsequent use.
Provided are a polypeptide and nucleic acid for encoding the polypeptide, a nucleic-acid construct, an expression vector, and a host cell containing the nucleic acid, an antigen-presenting cell presenting the polypeptide on the surface of the cell, and immune effector cell thereof, a pharmaceutical composition containing the polypeptide, a vaccine containing the nucleic acid, the nucleic acid construct, the expression vector, the host cell, the antigen-presenting cell, and the immune effector cell, and an antibody recognizing the polypeptide. Also provided is a therapeutic method using the polypeptide, the nucleic acid, the pharmaceutical composition, the vaccine, and the antibody. Also provided are a diagnosis method and diagnosis apparatus for detecting the described polypeptide. Also provided is an application of the polypeptide in preparing a vaccine, a tumor diagnosis kit, or a pharmaceutical composition, and an application of the polypeptide or the nucleic acid as a test target in tumor diagnosis.
Provided are a method for constructing a library based on an RNA sample and uses thereof. The method includes: step 1 of subjecting the RNA sample to a reverse transcription reaction to obtain DNA-RNA hybrid strands; step 2 of performing reaction of the DNA-RNA hybrid strands with an endoribonuclease, a first DNA polymerase, a second DNA polymerase, and dATPs to obtain a double-stranded DNA added with dA-tail, where the first DNA polymerase has a 5′-3′ exonuclease activity and a 3′-5′ exonuclease activity, and the second DNA polymerase has no 3′-5′ exonuclease activity; step 3 of ligating the double-stranded DNA added with dA-tail and a sequencing adaptor to obtain a ligated product; and step 4 of subjecting the ligated product to PCR amplification to obtain a sequencing library.
A modified nucleoside or nucleotide, the 3'-OH of the modified nucleoside or nucleotide being reversibly blocked; meanwhile, the present invention also relates to a kit comprising the nucleoside or nucleotide, and a sequencing method based on the nucleoside or nucleotide.
C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
61.
SNP MARKERS OF DRUG REDUCED SUSCEPTIBILITY RELATED EVOLUTIONARY BRANCHES OF CLOSTRIDIUM DIFFICILE, METHOD FOR IDENTIFYING STRAIN CATEGORY, AND USE THEREOF
Provided are SNP markers of drug reduced susceptibility related evolutionary branches of Clostridium difficile, a method for identifying the category of a Clostridium difficile strain, and use thereof. The SNP markers are specific markers of three categories of the Clostridium difficile clade2 (mainly hypervirulent ribotype 027), allowing for rapid and accurate identification of the evolutionary branches of Clostridium difficile strains that are resistant to a variety of therapeutic drugs and related drugs. Accurate categorization of the drug reduced susceptibility related evolutionary branches not only provides evidence for the evolutionary traceability of drug-resistant pathogens, but also offers effective and actionable guidance on clinical drug usage.
C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
62.
METHODS AND COMPOSITIONS FOR BIOLUMINESCENCE-BASED SEQUENCING
Disclosed herein are methods and compositions for corresponding positions on an array with differently labeled affinity reagents immobilized at the positions. A first affinity reagent is immobilized at least some of the first positions and a second affinity reagent is immobilized at least some of the second positions. The first affinity reagent is associated with a first luciferase polypeptide and the second affinity reagent is associated with a second luciferase polypeptide. The first luciferase polypeptide does not cross react with the second substrate and the second luciferase polypeptide does not cross react with the first substrate. The method further comprises contacting the array with the first substrate, which reacts with the first luciferase polypeptide and detecting the first luminescent signal at positions, at which the first affinity reagent is immobilized and contacting the array with the second substrate and detecting the second luminescent signal at positions, at which the second affinity reagent is immobilized, thereby corresponding positions on an array with the first affinity reagent or the second affinity reagent immobilized at the positions.
The present disclosure provides a fluorescence image registration method, the method includes: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring a position of a minimum value of a sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels; if the fluorescence image meets the preset standard, correcting positions of the defective pixels according to positions of the non-defective pixels; acquiring a position of a center of gravity of image points of fluorescent molecules according to a center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points.
A microbead with a code engraved on an outside of the microbead. The microbead includes a central region and an edge region surrounding the central region. An outer contour of the edge region before and after engraving the code is non-circular. The edge region includes a plurality of coding positions. The code of the microbead is engraved on the plurality of coding positions. Each bit of the code corresponds to each of the plurality of coding positions. The present disclosure increases the utilization rate of the microbead.
Megamonas funiformis has the function of preventing and/or treating metabolic diseases (such as obesity, diabetes, atherosclerosis-related diseases, cardiovascular disease and hyperuricemia).
A61P 9/10 - Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
A nucleic acid synthesis device and a nucleic acid purification device, uses thereof, and a nucleic acid synthesis method and a nucleic acid purification method. The nucleic acid synthesis device includes a solid support, and the solid support includes a controlled pore glass (CPG), the CPG is an unmodified and bare CPG, a surface of the CPG has a hydroxyl group, and the hydroxyl group is attachable, though a covalent bond, to a phosphoramidite-protected nucleotide monomer or multimer for synthesis of nucleic acid. The nucleic acid synthesis device of the present disclosure can be used for not only synthesis of an oligonucleotide primer, but also for purification of enzymatic digestion and PCR product by using the oligonucleotide primer immobilized on the CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.
A nanopore sequencing method, including: in a nanopore sequencing device, performing rolling circle amplification by using a single-strand ring or double-strand ring of nucleic acid as a template to produce an amplified single strand; under the action of an electric field, introducing the amplified single strand into nanopores of the nanopore sequencing device, so that the amplified single strand passes through the nanopores and generates an electrical signal for each base. By determining electrical signal differences caused by different bases, a base sequence on a template nucleic acid is measured.
Provided are a chip matrix, a sequencing chip, and a manufacturing method thereof. The chip matrix includes: a wafer layer (111), the wafer layer (111) having cutting lines that are evenly distributed thereon; a first silicon oxide layer (112), the first silicon oxide layer (112) being made of silicon oxide and formed on an upper surface of the wafer layer (111); a transition metal oxide layer (113), the transition metal oxide layer (113) being made of transition metal oxide and formed on an upper surface of the first silicon oxide layer (112). The chip matrix has characteristics such as resistances against high temperature, high humidity and other harsh environments. Meanwhile, by changing pH, surfactant and other components of a solution containing sequences to be sequenced, a surface functional region of the chip matrix can specifically adsorb a sequence to be sequenced.
H01L 21/02 - Manufacture or treatment of semiconductor devices or of parts thereof
H01L 21/78 - Manufacture or treatment of devices consisting of a plurality of solid state components or integrated circuits formed in, or on, a common substrate with subsequent division of the substrate into plural individual devices
70.
Nucleic acid sequencing method and nucleic acid sequencing kit
Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.
Anaerofustis stercorihominis in the prevention and/or treatment of metabolic diseases such as obesity, diabetes, atherosclerosis-related diseases, and cardiovascular diseases.
Provided are a Phi29 DNA polymerase mutant with improved thermal stability and an use thereof in sequencing. The phi29 DNA polymerase mutant is represented by A) or B) below: the DNA polymerase mutant represented by the A) is a protein having DNA polymerase activity that is obtained by modifying at least one amino acid residue(s) in the following six sites in a phi29 DNA polymerase amino acid sequence: the 97-th, 123-th, 217-th, 224-th, 515-th, and 474-th sites; the DNA polymerase mutant represented by the B) is a protein having DNA polymerase activity that is derived from the A) by adding a tag sequence to a terminal of the amino acid sequence of the protein represented by the A).
An integrated circuit for controlling a sensor chip capable of sensing various materials includes a plurality of amplifier clusters, a plurality of analog multiplexers, and at least one analog-to-digital converter coupled the analog multiplexers and configured to generate digital code values representative of electrical signals. Each of the amplifier clusters include four amplifiers, each amplifier has a first input coupled to a sensor of the sensor chip, and a second input coupled to a programmable voltage reference. Each one of the analog multiplexers is coupled to one of the amplifier clusters and configured to selectively pass through an electrical signal to the at least one analog-to-digital converter.
The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.
Provided is a recombinant KOD polymerase, which is the following A) or B): the polymerase shown in A) is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 18 positions in a wild-type KOD DNA polymerase amino acid sequence: 675th, 385th, 710th, 674th, 735th, 736th, 606th, 709th, 347th, 349th, 590th, 676th, 389th, 589th, 680th, 384th, 496th and 383rd; the polymerase described by B) is a protein having DNA polymerase activity that is derived from A) by adding a tag sequence to an end of the amino acid sequence of the protein shown in A).
A separation and collection device for cells and biomolecules includes an annular tube and several magnetic beads configured to identify and bind with different cells or molecules in the biological sample. The annular tube defines a through hole for the biological sample to exit. Sizes of different groups of magnetic beads are different. An aperture size of a through hole is changeable such that the plurality of groups of magnetic beads can flow through the through hole in a certain order. A testing system including the separation and collection device is further disclosed.
Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.
A method for constructing a library of cell-free DNAs in body fluids, comprising directly acting a transposase or an endonuclease on a body fluid sample, fragmenting the cell-free DNAs within, and performing amplification to obtain a library. Also provided is a test kit using the present method for prenatal diagnosis or early detection of cancer.
The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/6876 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
C07K 16/44 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere
C12Q 1/6804 - Nucleic acid analysis using immunogens
A61K 47/00 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additivesTargeting or modifying agents chemically bound to the active ingredient
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
80.
Anti-BCMA single-chain antibody scFv and preparation method and application thereof
Provided are an anti-BCMA single-chain antibody scFv and a preparation method and application thereof. The preparation method is capable of artificially synthesizing a BCMA-targeted phage display library by combining site-directed mutagenesis and random mutagenesis technologies; and acquiring 3 new-type BCMA antigen-targeted scFv strains to serve as BCMA-targeted antibodies by combining a phage display technology.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
81.
RAPID MULTIPLEXING CRISPR-BASED DIAGNOSTICS USING MICROFLUIDIC COMPARTMENTALIZATION
The invention provides a method for detecting the presence of a plurality of different target nucleic acids, comprising the following steps: distributing a portion of a composition comprising nucleic acid into each of a plurality of microfluidic compartments, introducing the Cas endonuclease reaction solution into individual compartments, and detecting the signal changes in the compartments.
A method for constructing a sequencing library, a sequencing library and a sequencing method. The construction method includes: cyclizing a linear nucleic acid molecule to form a circular nucleic acid molecule, performing rolling circle amplifying to obtain a multi-copy a long-fragment nucleic acid molecule, and then synthesizing a complementary strand to obtain a double-stranded long-fragment nucleic acid molecule; mixing and incubating with a transposition complex to form a long fragment nucleic acid molecule carrying the transposition complex, and mixing and incubating with a solid-phase carrier having a molecular barcode sequence to link the molecular barcode sequence to a transposon sequence on the transposition complex; releasing a transposase from the long-fragment nucleic acid molecule, and breaking the long fragment nucleic acid molecule into short-fragment nucleic acid molecules connected with the transposon sequence and molecular barcode sequence; performing polymerase chain amplification on the short nucleic acid molecule to obtain a sequencing library.
C07H 15/04 - Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of a saccharide radical
C07H 19/10 - Pyrimidine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
C07H 19/20 - Purine radicals with the saccharide radical being esterified by phosphoric or polyphosphoric acids
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Provided are compositions and methods directed to chemically synthesizing oligonucleotides using an array system that contains multiple subset arrays, in which each subset array comprises primers. Each subset array can be selectively immersed in coupling solutions to extend the primers.
Butyribacter intestini in the treatment and prevention of obesity-related diseases. Also provided is a composition for the treatment and prevention of obesity and obesity-related diseases.
A gene chip includes a chip carrier, a plurality of DNA nanoballs assembled on the chip carrier, and a polymer film formed on the chip carrier and wrapping the DNA nanoballs. The polymer film includes at least one of a film of a positively charged polymer, a film of a positively charged polymer which is modified, a film of a zwitterionic polymer, and a composite polymer film. The composite polymer film is formed by a layer-by-layer self-assembly process of a positively charged polymer and a negatively charged polymer. The gene chip has good sequencing quality and different functions can be achieved by coating with different polymers, such as the chip surface rapidly drying out and surface non-specific adsorption. A method of preparing a gene chip is further disclosed.
Provided are compositions and methods directed to using a short template and a polymerase or ligase to synthesize an oligonucleotide. The template is designed such that only one nucleotide is added to the primer in each round of synthesis.
C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
C40B 50/18 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support using a particular method of attachment to the solid support
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
A method for generating a digital identity is provided, including: extracting a first preset number of short tandem repeat STRs and relevant information of each STR from whole genome data; generating a single STR digital code corresponding to each STR according to the relevant information of each STR, to obtain a plurality of single STR digital codes; performing sequence transformation on each single STR digital code with a preset rule, and generating a target STR digital code according to the single STR digital code after the sequence transformation; generating summary information of the target STR digital code, and determining the summary information as summary information of the STR to which the target STR digital code belongs; and determining the summary information of the STR as the generated digital identity.
Methods and compositions for preparing a nucleic acid sequencing library are described including (a)transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.
C40B 50/16 - Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creationParticular methods of cleavage from the solid support involving encoding steps
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C40B 20/04 - Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
91.
INFORMATION ENCODING METHOD AND APPARATUS, INFORMATION DECODING METHOD AND APPARATUS, STORAGE MEDIUM, AND INFORMATION STORAGE AND INTERPRETATION METHOD
An information encoding method and apparatus, an information decoding method and apparatus, a storage medium, and an information storage and interpretation method. The information encoding method comprises: obtaining first binary information and second binary information as well as a first encoding rule and a second encoding rule, the first encoding rule being used for encoding the first binary information, and the second encoding rule being used for encoding the second binary information (S201); obtaining first output candidate symbols corresponding to the current input of the first binary information according to the first encoding rule, obtaining second output candidate symbols corresponding to the current input of the second binary information according to the second encoding rule, and taking an intersection of the first output candidate symbols and the second output candidate symbols as an output corresponding to the current input (S202); and sequentially determining an output symbol corresponding to each binary bit of the first binary information and the second binary information by means of the first encoding rule and the second encoding rule to obtain an encoding sequence composed of multiple output symbols (S203). According to the method, two pieces of input binary information are converted and integrated as one piece of information, the information capacity limit is reached, and the information encoding density is high.
Butyribacter intestini has the function of preventing and/or treating inflammation-related diseases (for example, inflammatory bowel disease, such as ulcerative colitis, gastritis, and common enteritis; and rheumatoid arthritis).
Embodiments include methods and apparatuses for separating beads from a droplet main body (46a) on a microfluidics actuator (40) by applying a magnetic field (49) to a droplet (46) disposed at a first location, the droplet (46) including one or more magnetically responsive beads; and moving the magnetic field (49) to separate the one or more magnetically responsive beads from a main body (46a) of the droplet (46). Embodiments also include methods and apparatuses for introducing one or more beads into a droplet main body (46a) by applying a magnetic field (49) to one or more magnetically responsive beads and moving the magnetic field (49) to introduce the one or more magnetically responsive beads into a droplet (46) disposed on a first location, wherein the droplet (46) includes a fluid.
A microfluidic device includes first and second substrate structures. The first substrate structure has a first substrate surface configured to receive one or more droplets. A plurality of electrodes configured to apply an electric field to the droplets. The second substrate structure has a second substrate surface facing the first substrate surface and spaced apart from the first substrate surface to form a fluid channel. The microfluidic device has a first heating element adjacent to the first substrate structure and disposed on an opposite side of the first substrate surface, and a second heating element adjacent to the second substrate structure and disposed on an opposite side of the second substrate surface. The microfluidic device further includes one or more temperature sensors disposed adjacent to the fluid channel between the first substrate structure and the second substrate structure.
Acridinium ester-containing compounds are provided herein. Also provided herein are chemiluminescence-based one-color sequencing methods and methods of using the acridinium ester-containing compounds in chemiluminescence-based one-color sequencing.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C07H 19/00 - Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radicalNucleosidesMononucleotidesAnhydro derivatives thereof
A method and device for displacing fluid from a reagent cartridge (310) into a microfluidic device (320) and for loading the fluid into the reagent cartridge (310). The reagent cartridge (310) may include a cartridge body and a pipette array with pipette tips (315) to engage inlets of a microfluidics or other cartridge, wherein the pipette tips (315) correspond in position to the plurality of inlets (325) of the microfluidic device (320). Fluid may be loaded into or displaced from the microfluidic device (320) by a system of plungers (615). The reagent cartridge may alternatively include blisters (925) having fluid reservoirs and dispensing tips (930), each dispensing tip (930) including a pathway (927) that is fluidly coupled to a blister (925). The fluid may be displaced from or loaded into the blister (925) via the dispensing tip (930). A deformable seal (910) may be overlaid on the blisters (925) to seal the volumes of fluid within the blisters (925), and may be deformed to displace the fluid.
EYE, EAR, NOSE, AND THROAT HOSPITAL OF FUDAN UNIVERSITY (China)
Inventor
Liu, Xiaoqing
Hong, Jiaxu
Ni, Yong
Li, Shuangshuang
Wang, Lili
He, Wei
Guo, Youwen
Liu, Yuxuan
Liu, Yong
Wang, Wei
Xu, Ruiqi
Cheng, Jingyi
Tian, Lijia
Chen, Wenbin
Xu, Xun
Abstract
The present disclosure proposes a modeling method and apparatus for diagnosing an ophthalmic disease based on artificial intelligence, and a storage medium. The modeling method includes: establishing a data collection of ophthalmic images and a data collection of non-image ophthalmic disease diagnosis questionnaires; training a first neural network model by employing the data collection of the ophthalmic images to obtain a first classification model; training a second classification model by employing the data collection of non-image ophthalmic disease diagnosis questionnaires; and merging the first classification model and the second classification model to obtain a target classification network model, in which, a test result outputted by the target classification network model is used as a diagnosis result of the ophthalmic disease.
G16H 50/20 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
G16H 10/20 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data for electronic clinical trials or questionnaires
A61B 3/12 - Objective types, i.e. instruments for examining the eyes independent of the patients perceptions or reactions for looking at the eye fundus, e.g. ophthalmoscopes
A61B 3/14 - Arrangements specially adapted for eye photography
G06F 18/214 - Generating training patternsBootstrap methods, e.g. bagging or boosting
G06F 18/21 - Design or setup of recognition systems or techniquesExtraction of features in feature spaceBlind source separation
G06V 10/764 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
G06V 10/80 - Fusion, i.e. combining data from various sources at the sensor level, preprocessing level, feature extraction level or classification level
G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts
Megamonas funiformis has the effects for preventing and/or treating inflammation-related diseases, such as inflammatory bowel diseases (such as ulcerative enteritis, gastritis, and general enteritis) and cardiovascular diseases.
The present disclosure relates to an encoding/decoding method, an encoder/decoder, and a storage method and device, which relates to the technical field of data storage. The encoding method comprises: determining a first bit of the encoded sequence based on a first bit of the first binary code sequence, a first bit of the second binary code sequence, and a reference symbol, the reference symbol being any one of the four different kinds of symbols; determining a current bit of the encoded sequence based on a current bit of the first binary code sequence, a current bit of the second binary code sequence, and a previous bit of the encoded sequence, the current bit of the encoded sequence being a bit other than the first bit of the encoded sequence.
Bacteroides cellulosilyticus in preparing a preparation for preventing and/or treating lipid metabolism related diseases, such as atherosclerosis related diseases, cardiovascular diseases and obesity.
A23K 50/50 - Feeding-stuffs specially adapted for particular animals for rodents
A23K 10/18 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics
A61P 9/10 - Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
patents
