Magnetic cells plus a magnet are used to treat ED and other bodily lesions, whereby the magnetic cells are held in at or near the location of the lesion with a magnetic field for a period of time until efficacy is established. The cells can be any cell type, including stem cells, autologous cells, recombinant cells, combinations thereof and the like.
A61L 15/36 - Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing microorganisms
Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same.
Specialized culture plates for imaging cells in a quick, high throughput manner are provided. Ideally the wells of the culture plate have triangular, square, or V-shaped wells or cell sorting walls having a plurality of vertices, and more complicated variations thereof are also possible. The plates are tilted or rotated to collect the cells at the vertex or vertices of the wells, optionally vibrated to speed the collection, then the vibration and tilt or rotation removed for some period of time, whereon the cells are imaged through the flat transparent bottom of the plate.
Methods of using magnetic display cells to replace secondary antibodies and magnetic beads in any cell manipulation methods. Cells displaying ligands for target cells are magnetized, and then used to bind the target cells. The complex can then be collected in a magnetic field, and thereby manipulated according to the application needs.
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
G01R 33/12 - Measuring magnetic properties of articles or specimens of solids or fluids
H01F 1/06 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
H01F 1/08 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder pressed, sintered, or bound together
H01F 1/113 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles in a bonding agent
H01F 1/44 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
6.
USE OF MAGNETIC CELLS TO MANIPULATE NON-MAGNETIC CELLS
Methods of using magnetic display cells to replace secondary antibodies and magnetic beads in any cell manipulation methods. Cells displaying ligands for target cells are magnetized, and then used to bind the target cells. The complex can then be collected in a magnetic field, and thereby manipulated according to the application needs.
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
G01N 27/00 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
Specialized culture plates for imaging cells in a quick, high throughput manner are provided. Ideally the wells of the culture plate have triangular, square, or V-shaped wells or cell sorting walls having a plurality of vertices, and more complicated variations thereof are also possible. The plates are tilted or rotated to collect the cells at the vertex or vertices of the wells, optionally vibrated to speed the collection, then the vibration and tilt or rotation removed for some period of time, whereon the cells are imaged through the flat transparent bottom of the plate.
Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.
Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats.
Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.
A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
B03C 1/015 - Pretreatment specially adapted for magnetic separation by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation
B03C 1/30 - Combinations with other devices, not otherwise provided for
B82Y 25/00 - Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/544 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
G01R 33/12 - Measuring magnetic properties of articles or specimens of solids or fluids
H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
H01F 1/08 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder pressed, sintered, or bound together
H01F 1/113 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles in a bonding agent
H01F 1/44 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
C40B 30/04 - Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G01N 33/566 - Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagent
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
H01F 1/44 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
H01F 1/08 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder pressed, sintered, or bound together
H01F 1/113 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles in a bonding agent
H01F 1/06 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
14.
Materials for magnetizing cells and magnetic manipulation
A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.
C12Q 1/02 - Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
C12N 13/00 - Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
C12N 11/00 - Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
G01R 33/12 - Measuring magnetic properties of articles or specimens of solids or fluids
B03C 1/015 - Pretreatment specially adapted for magnetic separation by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation
B03C 1/30 - Combinations with other devices, not otherwise provided for
B82Y 25/00 - Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
G01N 33/543 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/544 - Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
H01F 1/08 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder pressed, sintered, or bound together
H01F 1/113 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites in the form of particles in a bonding agent
H01F 1/44 - Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of magnetic liquids, e.g. ferrofluids
C12Q 1/24 - Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
C12N 1/02 - Separating microorganisms from their culture media
B82B 3/00 - Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
[ Active chemical ingredients for use in the manufacture of anti-cancer drugs; Antioxidants and proteins used in the manufacture of cosmetics, beverages, food products and food supplements; Artificial plant-cultivation soil; Biochemical reagents commonly known as probes, for detecting and analyzing molecules in protein or nucleotide arrays; ] Biological Tissue, namely, a set of matched frozen and fixed human biological specimens derived from the division of one original specimen for use in scientific and medical research; Biological tissue, namely, blood, stem cells, umbilical cords and placentas for scientific and medical research use; Biological tissue, namely, SHED stem cells (stem cells from human exfoliated deciduous teeth), dental pulp and human maxiofacial bone elements for scientific use and research in cellular healing therapies; Biomedical compounds, namely, peptide substrates used in analyzing and detecting certain toxins for clinical or medical laboratory use; Biomedical compounds, namely, peptide substrates used in analyzing and detecting certain toxins for laboratory or research use; [ Biotechnology formed genes, microbes and enzymes for use in the production of agricultural seeds to enhance plants' growth therefrom; Carbon nano particles, namely, carbon nano particles that photoluminesce; Cells for scientific, laboratory or medical research; Chemical additives for use as cleansing, deodorizing and refreshing ingredients in the manufacture of mouth sprays, dental cements and medicines for dental purposes; Chemical additives for use in the manufacture of pharmaceuticals, cosmetics; Chemical gel for use as an ice substitute; Chemical reagents for non-medical purposes; Chemically activated adhesive label for sensing the presence of oxygen in intravenous medical and pharmaceutical containers; Chemically treated non-medical test strips for calibration of laboratory test equipment; Chemicals for use in biotechnological manufacturing processes; Chemicals for use in biotechnological product development; Chemicals for use in the purification of proteins for in vitro use; ] Cultures of microorganisms other than for medical and veterinary use; Diagnostic kits consisting primarily of monoclonal antibodies, buffers, and reagents to monitor toxicity of drugs; Diagnostic preparations for scientific or research use; Diagnostic preparations for clinical or medical laboratory use; Diagnostic preparations for scientific use; Diagnostic preparations other than for medical or veterinary purposes; Diagnostic preparations used in science; Diagnostic reagents and preparations, except for medical or veterinary use; Diagnostic reagents for clinical or medical laboratory use; Diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; [ Electrophoresis gels other than for medical or veterinary purposes; Enzyme substrates; Enzymes derived from biotechnological processes for use in industry; Enzymes for use in protein hydrolysis; Food supplements for agricultural products and other plants; Growing media for plants; Immunohistochemistry detection kits consisting of mouse secondary reagents, DAB chromogens, DAB buffers, peroxidase blocking reagent, and hematoxylin, for anatomic pathology purposes in the medical diagnostics industry; Kit containing pre-packed columns, chemicals, pre-made buffer concentrates, syringes and instructional manual for the purification of proteins for in vitro use; Laboratory chemicals, namely, an antibody reagent used for the detection of antigens in cell and ] tissue analysis for in vitro diagnostic use [ ; Magnetic fluid for industrial purposes; Medical grade polyurethanes for use in the manufacture of medical tubing, films, sprays, and membranes; Nucleic acid isolation and purification kit consisting primarily of reagents and magnetic beads for scientific research purposes; Nucleic acid sequences and chemical reagents for other than medical and veterinary purposes; Nutritive additive to enhance the biological activity of water, soil, seeds and plants for purposes of fertilization and bioremediation of pollutants; Plant and herb extracts for use in the manufacture of cosmetics; Plant extracts for use in the manufacture of creams, lotions and cosmetic products; Plant extracts, namely, phytochemicals, used in the manufacture of medical products, creams, lotions, and cosmetic products; Plant food; Plant growth regulating preparations; Plant growth regulators for agricultural use; Plant hormones (phytohormones); Plant nutrition preparations; Polymer base compositions used in the manufacture of commercial, industrial and domestic goods; Polymer beads for use in manufacturing; Polymers and polymeric additives for use in the manufacture of industrial products, consumer and household products, sealants, and automotive fluids; Polymers and polymeric additives for use in the manufacture of pharmaceutical preparations, medical devices, plastics, cosmetics, personal care products, coatings, adhesives, and lubricants; Polymers and polymeric additives for use in the manufacture of pharmaceutical preparations, plastics, cosmetics, personal care products, coatings, adhesives, and lubricants; Preparations for fortifying plants; Protein arrays and nucleotide arrays for scientific and medical research; Reagent for chemical analyses; Reagent kits comprising generic DNA circle, DNA primers, polymerase and buffers for use in biotechnology fields; Reagents and substrates, namely, chemical compounds for use in patterning at nano scale or near nano scale; Reagents for research purposes; Reagents for scientific or medical research use; Reagents for testing the sterility of medical equipment; Reagents for testing the sterility of pharmaceuticals and injectable solutions; Reagents for use in scientific apparatus for chemical or biological analysis; Reagents used for analytical moisture determinations; Research reagents for industrial use, namely, for food testing; Silica gel; Stem cells for research or scientific purposes; Stem cells for research purposes; Substances for regulating plant growth; Synthetic amorphous silica gel for further processing in connection with the manufacture of finished goods; Testing kits containing peptide substrates used in analyzing and detecting certain toxins for clinical or medical laboratory use; Testing kits containing peptide substrates used in analyzing and detecting certain toxins for laboratory or research use ]
