Abstract

The present invention relates to a method for the in vitro diagnosis of stroke and transient ischemic attack (TIA) in an individual, comprising the following steps: (a) measuring the level of proBNP(1 -108), or of fragments of proBNP(1 -108) comprising a RAPRSP sequence (SEQ ID NO: 1 ), in a biological sample of the individual; (b) comparing the measured level with a cut-off value; (c) determining therefrom whether a stroke or a TIA has occurred in the individual.

IPC Classes  ?

• C07K 14/58 - Atrial natriuretic factor complexAtriopeptinAtrial natriuretic peptide [ANP]CardionatrinCardiodilatin
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to a method for the in vitro diagnosis of stroke and transient ischemic attack (TIA) in an individual, comprising the following steps: (a) measuring the level of proBNP(1 -108), or of fragments of proBNP(1 -108) comprising a RAPRSP sequence (SEQ ID NO: 1 ), in a biological sample of the individual; (b) comparing the measured level with a cut-off value; (c) determining therefrom whether a stroke or a TIA has occurred in the individual.

IPC Classes  ?

• C07K 14/58 - Atrial natriuretic factor complexAtriopeptinAtrial natriuretic peptide [ANP]CardionatrinCardiodilatin
• G01N 33/74 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving hormones

Abstract

The invention relates to a method for measuring the ratio of nucleic acids of a target sequence in a sample of interest. The method comprises a plurality of steps, in particular: submitting the sample of interest to an amplification processing in the presence of at least one nucleic-acid marker specific to said target sequence; measuring a physical value representative of the variation in the marker; calculating a parameter Fo representative of the physical value of the nucleic acid marker before any amplification cycle; estimating the initial nucleic-acid population of the target sequence using a conversion rule that comprises contextual parameters and is applied to said parameter Fo. The contextual parameters are pre-recorded reference parameters which are substantially independent from at least a portion of the experimental conditions. The invention also relates to an apparatus including units adapted for carrying a method for measuring the ratio of nucleic acids of a target sequence in a sample of interest.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G06F 19/00 - Digital computing or data processing equipment or methods, specially adapted for specific applications (specially adapted for specific functions G06F 17/00;data processing systems or methods specially adapted for administrative, commercial, financial, managerial, supervisory or forecasting purposes G06Q;healthcare informatics G16H)

Abstract

The present invention relates to a polypeptide carrying a human BNP(I -32) epitope according to Formula (I): a1-R1-X1-FGRKMDR-X2-R2-a2 as well as ligands specific of the FGRKMDR epitope.

IPC Classes  ?

• C07K 14/58 - Atrial natriuretic factor complexAtriopeptinAtrial natriuretic peptide [ANP]CardionatrinCardiodilatin
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.

IPC Classes  ?

• G01N 33/80 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving blood groups or blood types
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/537 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The invention relates to a method for the in vitro diagnostic detection of an infection with a microorganism, comprising placing a biological sample, in a single assay receptacle, in the presence of particles, each carrying at least one specific detectable physical parameter, and belonging to at least two different groups, one of the groups carrying an anti-IgM capture antibody and the other group carrying a capture antigen derived from said microorganism.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/576 - ImmunoassayBiospecific binding assayMaterials therefor for hepatitis

Abstract

The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.

IPC Classes  ?

• G01N 33/80 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving blood groups or blood types
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/537 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present invention relates to a double-stranded probe intended for the fluorescent detection of at least one single-stranded or double-stranded target nucleic acid, comprising: - a first strand of formula X1-(L1)a-S1-S'1-(L'1)b-Y1 intended for the detection of a first strand of the target nucleic acid which comprises a sequence of formula T1-T1; - a second strand of formula X2-(L2)c-S2-S'2-(L'2)d-Y2 intended for the detection of a second strand of the target nucleic acid, if present, said second strand of the target nucleic acid comprising a sequence of formula T2-T2; wherein two of X-1, X2, Y1, and Y2 represent a fluorescent donor, while the two others represent a fluorescent acceptor, and X1 and Y2 can not both represent a fluorescent donor.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The invention relates to the detection of Salmonella by nucleic acid amplification. The invention provides primer and probe oligonucleotides that can be used in multiplex to detect Salmonella in real-time amplification. The oligonucleotides of the invention detect all group I serovars, and have an increased Salmonella detection range: they enable to cover the seven Salmonella groups. They also have an increased sensitivity, without loss in specificity.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The present invention relates to a method for the prognosis of a vascular event or the diagnosis of ACS in a patient suspected of being at risk for a vascular event or condition, said patient presenting: - no elevation of the ST segment as seen on an electrocardiogram, and/or - a normal level of at least one necrosis marker, wherein the presence and/or levels of at least two different biochemical markers are measured in a biological sample of said patient, whereby the probability that the patient will experience a vascular event or the vascular-related condition is deduced from the measured presence and/or levels of the biochemical markers such as MPO, sCD40L, IL-6, PaPP-A, CRP, D-dimer, troponin, and proBNP.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The invention relates to the detection of three different bacterial species which are responsible for sexually-transmitted diseases, i.e., Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG). The invention more particularly relates to the detection of these three species in real-time PCR, in multiplex PCR and in real-time multiplex PCR. The invention provides reference templates sequences, which are especially adapted to the design of primers and probes, which can be used together in the same tube to detect CT and/or MG and/or NG by real-time multiplex amplification.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The present invention relates to amplification primers and detection probes, which are useful for the detection of human papillomaviruses (HPV), and more particularly of HPV, which can be oncogenic for the mucosal epithelia. The amplification and detection systems provided by the present invention are group-targeted systems, namely A5-, A6-, A7-, and A9-targeted systems. The amplification and detection systems of the invention allow for an amplification of HPV in multiplex as well as for a real-time detection, whereby at least the thirteen HR HPV can be detected in a single-tube assay. The invention further allows for a reliable quantitation of HPV viral loads in real-time multiplex amplification.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

The invention concerns a method for identifying the genotype in position 171 of the sheep PrP as well as a method for selecting a sheep for reproduction. The invention also concerns kits for implementing said methods. The inventive identifying method includes steps of treating an ovine body fluid sample to be tested containing the PrP with a denaturing and reducing solution, immobilizing, optionally by a ligand, the denatured and reduced PrP on a solid phase, contacting the denatured, reduced and immobilized PrP with at least one detecting antibody, and detecting the possible presence of said at least one detecting antibody, one of the ligand or of said at least one detecting antibody specifically binding to the PrP having a particular allelic state in position 171. The invention is applicable in particular in the medical field, more particularly veterinary and sanitary medicine.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The present invention relates to the use of both RD9 and IS6110 as nucleic acid targets, for the specific and sensitive detection of a mycobacterium of the Mycobacterium tuberculosis complex (MtbC), and/or for the specific and sensitive discrimination of Mycobacterium tuberculosis and Mycobacterium canetti on one hand, from the other strains of said MtbC on the other hand. Advantageously, said MtbC detection and said species discrimination can be made in a single amplification run (multiplex PCR). The means of the invention are advantageous tools for the diagnosis of tuberculosis.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

The reaction vessel support comprises at least one support plate (46, 48) for accommodating one or more reaction vessels and is mounted so that it rotates about a rotation axis (R). According to one aspect of the invention, the support comprises at least one controlling element (94, 96) having releasable coupling means (100) with a moving actuating element (40) of an actuator (10), whereby permitting the controlling element (94, 96) to be displaced with the aid of the actuator (10), the controlling element (94, 96) and the support plate (46, 38) being connected to one another so that a displacement of the controlling element (94, 96) by the actuator (10) is capable of causing the support plate (46, 84) to rotate about the rotation axis (R) in at least one direction of rotation. The device is for use, particularly in microtitration plate supports comprising wells arranged in a matrix.

IPC Classes  ?

• G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations