Abstract

Provided are a method for preparing a ribonucleoside or a derivative thereof, and a biological enzyme preparation and an application thereof. The method comprises the following steps: in the presence of a biological enzyme preparation, respectively, successively, or simultaneously subjecting one or more substrates to an enzymatic reaction, wherein a substrate comprises one or more ribonucleotides having an oxidized nitrogen-containing aromatic heterocyclic group, or a salt thereof, and a glucogenic amino acid or a salt thereof. The method can be simultaneously applied to various enzymatic reactions taking ribonucleoside and derivatives thereof as raw materials and coenzymes, thereby becoming a lower-cost high-efficiency enzymatic technical platform.

IPC Classes  ?

• C12P 19/40 - Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
• C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/88 - Lyases (4.)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
• C12N 9/90 - Isomerases (5.)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

Provided herein are an oral care additive, an oral care composition, a method of preparing the same, an oral care kit and use thereof. The main ingredient of the oral care additive contains one or more of nicotinic acid, nicotinamide or derivatives thereof to supplement and increase the level of β-nicotinamide adenine dinucleotide in the oral cavity, and various vitamin and coenzyme may be included as well to complement the function of the main ingredient. The additives and the application methods of the present invention retain the functionality of the existing oral care products while adding new efficacy, thereby providing a more comprehensive oral health management experience for the user.

IPC Classes  ?

• A61K 8/67 - Vitamins
• A23G 4/12 - Chewing gum characterised by the composition containing microorganisms or enzymesChewing gum characterised by the composition containing paramedical or dietetical agents, e.g. vitamins
• A61K 8/04 - DispersionsEmulsions
• A61K 8/66 - Enzymes
• A61Q 11/00 - Preparations for care of the teeth, of the oral cavity or of dentures, e.g. dentifrices or toothpastesMouth rinses

Abstract

A membranous immobilized cell, polypeptide, oligopeptide or protein and a preparation method thereof are provided. The method includes the following steps: 1) providing un-film-form chitosan, where the chitosan is un-pre-crosslinked or pre-crosslinked; 2) providing a mixture of the un-film-form chitosan and cell, polypeptide, oligopeptide or protein, and in the mixture, the un-film-form chitosan is in a dissolved state; 3) mixing the mixture with a crosslinking reagent to obtain a co-crosslinked product of the chitosan and the cell, polypeptide, oligopeptide or protein; and 4) drying the co-crosslinked product to obtain membranous immobilized cell, polypeptide, oligopeptide or protein. When un-pre-crosslinked chitosan is used in the step 1), the method further includes comprises the step 5) mixing the membranous immobilized cell, polypeptide, oligopeptide or protein with phosphate salt, so that chitosan molecules therein are crosslinked with each other.

IPC Classes  ?

• C12N 11/10 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/92 - Glucose isomerase

Abstract

An oral care additive, an oral care composition, a preparation method therefor, an oral care kit, and an application of the oral care additive. The main component of the oral care additive comprises one or more nicotinic acid, nicotinamide or derivatives thereof to supplement and enhance the level of β-nicotinamide adenine dinucleotide in the oral cavity; and the component of the combination may also comprise both various vitamins and coenzymes to assist the functions of the main component. According to the additive and an application method therefor, the functions of an existing oral care product are reserved, and a brand-new effect is added, thereby promoting a user to have more comprehensive oral health management experience.

IPC Classes  ?

• A61P 1/02 - Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
• A61Q 11/00 - Preparations for care of the teeth, of the oral cavity or of dentures, e.g. dentifrices or toothpastesMouth rinses
• A61K 8/67 - Vitamins
• A61K 8/49 - Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
• A23G 4/12 - Chewing gum characterised by the composition containing microorganisms or enzymesChewing gum characterised by the composition containing paramedical or dietetical agents, e.g. vitamins
• A23G 4/06 - Chewing gum characterised by the composition

Abstract

Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

IPC Classes  ?

• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 11/02 - Enzymes or microbial cells immobilised on or in an organic carrier
• B01J 19/22 - Stationary reactors having moving elements inside in the form of endless belts
• C07K 1/04 - General processes for the preparation of peptides on carriers
• C07K 17/06 - Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/26 - Inoculator or sampler

Abstract

Provided are a method and a detection tool for detecting a physiologically active substance. The method for detecting the physiologically active substance comprises: catalyzing a physiologically active substance in a sample by using a biological enzyme to generate a color change trigger, so as to obtain and display a detection result. The method for detecting the physiologically active substance and the use thereof break through the limitation in normal detection of a medical instrument needing to be used, and the method is convenient and easy to implement.

IPC Classes  ?

• G01N 21/78 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour

Abstract

Provided is a method for preparing a mononucleotide of nicotinic acid or a derivative thereof, comprising: carrying out, in the presence of a phosphate donor, biological enzyme catalyzed reaction on a reaction substrate containing a hexose source and nicotinic acid or the derivative thereof to generate the mononucleotide of nicotinic acid or the derivative thereof; and using the mononucleotide as an intermediate to prepare a biological products such as nucleoside of the nicotinic acid or the derivative thereof.

IPC Classes  ?

• C12P 19/30 - Nucleotides

Abstract

Provided are a method for preparing β-nicotinamide mononucleotide, and an enzyme composition and an application thereof. The method comprises the following steps: 1) providing an extract of a microorganism, wherein the extract of the microorganism comprises oxidized nicotinamide adenine dinucleotide; and 2) mixing nicotinamide adenine dinucleotide diphosphatase with the extract of the microorganism, and enabling nicotinamide adenine dinucleotide diphosphatase to react with oxidized nicotinamide adenine dinucleotide to generate β-nicotinamide mononucleotide and AMP, wherein the amino acid sequence of nicotinamide adenine dinucleotide diphosphatase is represented by SEQ ID NO.1. The method can improve the productivity, can also achieve green production, and creates considerable economic outcomes on a whole.

IPC Classes  ?

• C12P 19/30 - Nucleotides

Abstract

A method for preparing nucleoside of nicotinic acid or a derivative thereof, nicotinate adenine dinucleotide, and nicotinic acid mononucleotide, an enzyme composition, and an application. The method for preparing nucleoside of nicotinic acid or a derivative thereof comprises: using 5'-nucleotidase to react with mononucleotide or a salt thereof of nicotinic acid or a derivative thereof as a substrate, wherein the amino acid sequence of the 5'-nucleotidase is as shown in SEQ ID NO. 1. The method is safer and more reliable than a chemical synthesis process, and since use of an organic solvent can be avoided, the method is more environment-friendly. Nicotinamide nucleoside can be more efficiently produced to cope with increasing demands.

IPC Classes  ?

• C12P 19/38 - Nucleosides
• C12P 19/30 - Nucleotides
• C12P 19/36 - Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)

Abstract

Provided are an enzymatic reaction composition, a method for increasing the amount of adenosine triphosphate (ATP) in an enzymatic reaction, and a method for synthesizing amino acids or derivatives thereof, polypeptides, enzymes or proteins by using ATP. In the method, a first enzyme or enzyme group for producing adenosine monophosphate (AMP) is added during the enzymatic reaction so as to additionally increase the amount of ATP.

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

Provided are a membrane-shaped immobilized cell, a polypeptide, an oligopeptide or a protein, and a preparation method. The preparation method comprises: 1) providing chitosan without membrane formation; 2) providing a mixed solution of the chitosan without membrane formation and a cell, polypeptide, oligopeptide or protein; 3) mixing a cross-linking agent with the mixed solution; and 4) drying a co-crosslinked product to obtain a membrane-shaped immobilized cell, polypeptide, oligopeptide or protein, wherein, when chitosan without pre-crosslinking is used in step 1), the method further comprises 5) mixing the membrane-shaped immobilized cell, polypeptide, oligopeptide or protein with a phosphate to cross-link chitosan molecules therein.

IPC Classes  ?

• C12N 11/02 - Enzymes or microbial cells immobilised on or in an organic carrier

Abstract

An anti-ageing composition, a drug and a healthcare product comprising same, and an application therefor, the active ingredients therein being nicotinamide mononucleotide and S-adenosylmethionine salt, and the molar ratio thereof being 1: (0.2-3.0).

IPC Classes  ?

• A61K 31/706 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
• A61K 31/7076 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
• A61P 39/00 - General protective or antinoxious agents
• A61P 3/00 - Drugs for disorders of the metabolism
• A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives

Abstract

Disclosed are a hair care composition and a preparation method therefor. The hair care composition comprises a nicotinamide mononucleotide or a salt thereof or a hydrate thereof, and a basic ingredient for stabilizing the nicotinamide mononucleotide or the salt thereof or the hydrate thereof. The ingredients of the hair care composition can improve the stability of the nicotinamide mononucleotide in liquids, can take solving the aging and growth problems of hair and body hair and the stability of the nicotinamide mononucleotide in liquids in a new direction, and can bring about diversified breakthroughs in the application of the nicotinamide mononucleotide.

IPC Classes  ?

• A61K 8/60 - SugarsDerivatives thereof
• A61Q 7/00 - Preparations for affecting hair growth
• A61Q 5/00 - Preparations for care of the hair
• A61Q 19/08 - Anti-ageing preparations

Abstract

Provided are an enzymatic reaction composition, a method for increasing the amount of adenosine triphosphate (ATP) in an enzymatic reaction, and a method for using ATP to synthesize amino acids or derivatives, polypeptides, enzymes or proteins thereof. In the method, a first enzyme or group of enzymes for producing adenosine monophosphate (AMP) is added during an enzymatic reaction so as to newly increase the amount of ATP.

IPC Classes  ?

• C12P 19/32 - Nucleotides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same-ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Goods & Services

Dental lacquer; Depuratives for the body; Dietary food supplements; Dietary supplements for animals; Dietetic foods adapted for medical purposes; Enzyme dietary supplements; Enzyme food supplements; Enzyme preparations for medical purposes; Nutritional supplements; Vitamin preparations

Abstract

This invention provides deacetoxycephalosporin C hydroxylase mutants, their encoding DNA sequences, the methods to utilize them and their application. The deacetoxycephalosporin C hydroxylase mutants are characterized by at least one amino acid mutation at residue position selected from Glycine at position 29, Alanine at position 40, Glycine at position 41, Arginine at position 182 and Threonine at position 272, based on the amino acid sequence shown in SEQ ID NO.:2 as reference sequence, and wherein the deacetoxycephalosporin C hydroxylase mutant has at least 10% increase in activity and at least 150% increase in thermostability compared to wild-type deacetoxycephalosporin C hydroxylase. The deacetoxycephalosporin C hydroxylase mutants in this invention have increased activity and thermostability, allowing them to be more commercially and industrially viable.

IPC Classes  ?

• C12P 35/02 - Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
• C12N 15/52 - Genes encoding for enzymes or proenzymes

Abstract

Provided herein are penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application. The penicillin expandase mutants using SEQ ID NO.: 2 in the Sequence Listing as a reference sequence, have at least one amino acid mutation at residue positions corresponding to threonine at position 42 and glutamine at position 126, wherein, amino acid at position 42 is substituted by any other natural amino acid except threonine, amino acid at position 126 is substituted by any other natural amino acid except glutamine. The penicillin expandase mutants of present invention have increased its thermostability and catalytic activity; it is more suitable for commercial and industrial applications.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12P 35/00 - Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
• C12P 37/00 - Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin

Abstract

A cephalosporin hydroxylase mutant, encoding DNA of the mutant, a method for using the mutant and an application thereof. An amino acid sequence of the cephalosporin hydroxylase mutant uses SEQ ID NO:2 as a reference sequence in a sequence listing, and has a mutation located in at least one of position 29 glycine, position 40 alanine, position 41 glycine, position 182 arginine and position 272 threonine; compared to wild-type cephalosporin hydroxylase, the cephalosporin hydroxylase mutant has an enzymatic activity at least 10% higher and a heat resistance at least 150% higher.

IPC Classes  ?

• C12N 15/53 - Oxidoreductases (1)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12P 35/02 - Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position

Abstract

Provided herein are a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

IPC Classes  ?

• C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12N 11/02 - Enzymes or microbial cells immobilised on or in an organic carrier
• B01J 19/22 - Stationary reactors having moving elements inside in the form of endless belts
• C07K 1/04 - General processes for the preparation of peptides on carriers
• C07K 17/06 - Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/26 - Inoculator or sampler

Abstract

Provided is a penicillin expandase mutant, wherein same takes SEQ ID NO: 2 of the sequence listing as the reference sequence, and has the mutations of at least one of threonine at position 42 and glutamine at position 126. Also provided are the DNA encoding the mutant, and the reagent kit containing the mutant and the use thereof. The penicillin expandase mutant has an improved catalytic activity and heat resisting property.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/53 - Oxidoreductases (1)
• C12P 35/00 - Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Abstract

The invention provides a device and a method for preparation of immobilized proteins, enzymes or cells on a carrier to achieve the industrial batch production of the immobilized proteins, enzymes or cells.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

Provided are a composite carrier for immobilization of a protein, polypeptide or oligopeptide, a preparation method and an application thereof. The composite carrier is a porous material which comprises: (1) a porous organic foam material containing open pores; and (2) a crosslinked product having aldehyde groups and immobilized on the surface of the walls of one or more pores of the porous organic foam material, wherein the aldehyde groups are able to react with the protein, polypeptide or oligopeptide, and the crosslinked product are formed by chitosan via a crosslinking reaction with a polyaldehyde compound.

IPC Classes  ?

• C12N 11/08 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
• B01D 15/08 - Selective adsorption, e.g. chromatography
• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C07K 17/06 - Peptides being immobilised on, or in, an organic carrier attached to the carrier via a bridging agent
• C07K 17/08 - Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer
• B01J 20/26 - Synthetic macromolecular compounds

Abstract

Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/92 - Glucose isomerase
• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin

Abstract

The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes.

IPC Classes  ?

• C12N 11/00 - Carrier-bound or immobilised enzymesCarrier-bound or immobilised microbial cellsPreparation thereof
• C12N 11/08 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions

Abstract

Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity obtained by using recombinant techniques. These mutants comprise at least one amino acid variation at position 87, position 139, position 182, position 187, position 217, position 260, position 276, or position 299, and can be used in the conversion of hemicellulose to ethanol.

IPC Classes  ?

• C12P 7/06 - Ethanol, i.e. non-beverage
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/90 - Isomerases (5.)
• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.

IPC Classes  ?

• C12P 19/40 - Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
• C12N 9/10 - Transferases (2.)
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase

Abstract

A solid catalyst reactive arrangement comprises a reactive vessel and a solid catalyst within the vessel, wherein the solid catalyst has a first end-face, a second end-face and a periphery extending between the two end-faces and being enwrapped with a waterproofing membrane. A running method of the arrangement comprises injecting reactive fluids into the reactive vessel, wherein the fluids enter into the solid catalyst via its first end-face, are confined by the waterproofing membrane and flow toward the second end-face of the catalyst while reacting with the catalyst, then the resulting solution effuses from the second end-face of the catalyst. The arrangement and the running method enable to assemble and disassemble the solid catalyst easily, avoid the short circuit due to the effusion of reactive fluids from the periphery and the deformation/shrinkage of the catalyst during running, then improve reactive efficiency and running efficiency.

IPC Classes  ?

• B01J 8/02 - Chemical or physical processes in general, conducted in the presence of fluids and solid particlesApparatus for such processes with stationary particles, e.g. in fixed beds
• C12N 11/00 - Carrier-bound or immobilised enzymesCarrier-bound or immobilised microbial cellsPreparation thereof

Abstract

Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/92 - Glucose isomerase
• C12N 1/20 - BacteriaCulture media therefor
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

S-adenosylmethionine synthetase mutants with high heat-resistant and catalytic activity derived from Methanococcus jannaschii modified by gene mutation technique, characterized in that they have mutations in one or more amino acids chosen from amino acid residues corresponding to position 102, 93, 230 and 357 of Seq Id No.2 annexed to the specification. Using adenosine triphosphate (ATP) and methionine as substrates, they have at least 70% catalytic activity higher than the parent.

IPC Classes  ?

• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/10 - Transferases (2.)
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/54 - Transferases (2)

Abstract

A method for preparing high concentration fructose-containing high fructose syrup. The method comprises: using fructose-rich syrup having fructose content of more than 42% and solid content of 70-75% by weight as the raw material, adding crystal seeds and cooling to crystallize, separating the sediment by centrifuging or squeezing, thereby obtaining high fructose syrup having high concentration fructose in which fructose content is increased to 58-68.6%. The method of the invention is simple and easy to operation, has high yield, can obtain high fructose content, and doesn't need fructose-rich syrup having too high fructose content as the raw material.

IPC Classes  ?

• C13K 11/00 - Fructose
• C13K 1/10 - Crystallisation
• C13K 3/00 - Invert sugarSeparation of glucose or fructose from invert sugar

Abstract

A method for simultaneously preparing high fructose syrup having fructose content of 55% and 42% by weight. The method comprises : using fructose-rich syrup having fructose content of at least 48-52% and solid content of 70-75% by weight as the raw material, adding crystal seeds and cooling to crystallize, separating the sediment by centrifuging or squeezing, thereby obtaining a high fructose content syrup product in which fructose content is increased to 58-68.6%, then blending the product with said fructose-rich syrup raw material to form high fructose syrup having fructose content of 55%, simultaneously blending the sediment thus obtained with said raw material or said product to form high fructose syrup having fructose content of 42%.

IPC Classes  ?

• C13K 11/00 - Fructose
• C13K 1/10 - Crystallisation
• C13K 3/00 - Invert sugarSeparation of glucose or fructose from invert sugar

Abstract

A porous organic foam material used as the carrier of immobilized enzyme/cell and a method for making the related immobilized enzyme/cells. Enzyme protein/immobilized cells is/are fixed on the porous organic foam material by flocculating and crosslinking, the specific surface area of the product produced as above is bigger, the specific activity is higher, and the product can be made into plenty of kinds of shapes.

IPC Classes  ?

• C12N 11/06 - Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
• C12N 11/08 - Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
• C12N 9/92 - Glucose isomerase
• C12R 1/19 - Escherichia coli

Abstract

A series of glucose isomerase mutants derived from Thermoanaerobacterium saccharolyticum, which are modified by gene mutation technology. These mutants have mutations at residues 139, 182, 187, 299, which are mutated into Phe, Ala, Ser, Gln respectively, and also have at least one mutation selected from the positions 87,217,260 and 276. The substrate of these mutants is D-glucose. Compared with the wild type, they have improved catalytic activity of glucose isomerase. They can be used directly to prepare the syrup with high content of high fructose syrup (at least 55 %).

IPC Classes  ?

• C12N 9/92 - Glucose isomerase
• C12N 15/61 - Isomerases (5)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

Use of a series of glucose isomerase mutants derived from Thermoanaerobacterium saccharolyticum with high catalytic activity, which are modified by gene mutation technology. These mutants can be used to convert hemicellulose into ethanol. They have at least one mutation selected from the positions 87,139, 182, 187, 217,260,276 and 299.

IPC Classes  ?

• C12N 9/92 - Glucose isomerase
• C12N 15/61 - Isomerases (5)
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 19/24 - Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose

Abstract

A DNA sequence is for a recombinant D-amino acid oxidase with catalytic activity on cephalosporin C that is not less than 25% higher than that of its parent.

IPC Classes  ?

• C12N 15/53 - Oxidoreductases (1)
• C12P 35/00 - Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
• C07K 14/39 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts
• C12R 1/645 - Fungi
• C07D 501/06 - Acylation of 7-aminocephalosporanic acid

Abstract

The present invention discloses Escherichia coli expression vector, as well as a method for steadily, high-efficiently expressing a required target protein in Escherichia coli using the expression vector. The expression vector is constructed from vector pRSET-A, wherein ampicillin resistance gene in said vector pRSET-A is replaced by kanamycin resistance gene. The expression vector can be used to high-efficiently express enzyme required for the production of 7-aminocephalosporanic acid, meanwhile it does not produce &bgr;-lactamase by which the productivity of 7-aminocephalosporanic acid is reduced. The present invention also discloses two expression vectors, i.e., one expression vector pHS-GHA whose gene product is D-amino acid oxidase mutant GHA, and another expression vector pT7-kan-ACY whose gene product is glutaryl-7-aminocephalosporanic acid acylase of Pseudomonas sp. SE83.

IPC Classes  ?

• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12N 15/68 - Stabilisation of the vector
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/14 - Hydrolases (3.)
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12P 35/06 - Cephalosporin CDerivatives thereof

Abstract

The present invention discloses a two-steps enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, Wherein D-amino acid oxidase used is D-amino acid oxidase mutant of purified yeast Trigonopsis Variabilis, which having a specific activity more than 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, &bgr; -lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%.

IPC Classes  ?

• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/14 - Hydrolases (3.)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12P 35/06 - Cephalosporin CDerivatives thereof