Systems and methods for imaging and tracking fibrin formation via interaction of a test sample with a clotting agent or for imaging and tracking fibrin removal by an anti-clotting agent are described.In certain embodiments, the systems (200) comprise a planar reflective substrate (222, 224) comprising one or more capture agents and/or one or more fibrin reference regions; a mount for holding the substrate; an illumination light source (201) for directing illumination light toward a top surface of the substrate with fibrin (226)formed thereon; an image detector (232, 234) aligned with respect to the mount for detecting a portion of the illumination light that is scattered by the fibrin, and/or reflected by the reflective substrate, thereby obtaining a label-free image of fibrin formation or fibrin removal; a processor of a computing device (240); and a memory having instructions stored thereon, wherein the instructions, when executed by the processor, cause the processor to: receive and/or access data corresponding to the one or more label free images, and use the one or more label-free images to determine one or more measures of fibrin formation or fibrin removal.
Various embodiments of fluidic devices and methods of the present teaching can provide precision on-device loading of fluidic samples, and merging, mixing, and splitting of the fluidic samples, in illustrative embodiments as droplets, using pressures that can be provided by standard laboratory liquid handling equipment. Various embodiments of fluidic devices of the present teachings can provide on-device manipulation of accurate and precise fluidic volumes at the picoliter to nanoliter scale for each steps from fluidic sample loading to fluidic sample splitting. Various embodiments of fluidic elements of the present teachings, for example, but not limited by, various embodiments of fluidic traps of the present teachings, can have a constrained and measurable geometry, allowing for accurate and precise tuning of each fluidic sample volume throughout the on-device liquid handling process.
Described herein are fluidic devices and fluidic device assemblies, including microfluidic devices and cartridges comprising the same, that may be used to make particles or protein precipitates, or to monitor precipitate formation. The fluidic devices may include channels that connect a reaction well to an inlet port and an outlet port, and a fluidic constriction channel that may be configured to help retain fluids in the reaction well and/or promote mixing within the reaction well. In some variations, fluidic devices may be interconnected into fluidic assemblies that may be used in continuous process methods.
B01J 8/18 - Chemical or physical processes in general, conducted in the presence of fluids and solid particlesApparatus for such processes with fluidised particles
G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials
4.
Fluidic devices with reaction wells and constriction channels and uses thereof
The present disclosure provides fluidic devices and fluidic device assemblies, including microfluidic devices and cartridges comprising the same, that in illustrative embodiments, can be used to make particles or protein precipitates, or to monitor precipitate formation. The fluidic devices typically include channels that connect a reaction well to an inlet port and an outlet port, and a fluidic constriction channel that is configured to help retain fluids in the reaction well and/or promote mixing within the reaction well. In some aspect, fluidic devices are interconnected into fluidic assemblies that can be used in continuous process methods.
09 - Scientific and electric apparatus and instruments
Goods & Services
Laboratory equipment comprising computer hardware, downloadable software, recorded software, and flat panel display screens for use in measuring the viscosity and shear rates of fluids containing biologics, gene therapies, nanoparticles, formulations and other aqueous drug products
A spectral reflectance imaging device for detecting nanoparticle exosome biomarker targets includes an illumination source that illuminates a substrate with a plurality of separate wavelengths of incoherent light. The substrate includes an oxide layer and a binding agent to selectively bind nanoparticle exosome biomarker targets to the substrate. An imaging device bindings the light reflected from or transmitted through the substrate and an image processing system detects the nanoparticle exosome biomarker targets a function of the change in reflective properties of the substrate.
G01N 21/25 - ColourSpectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
G01N 21/45 - RefractivityPhase-affecting properties, e.g. optical path length using interferometric methodsRefractivityPhase-affecting properties, e.g. optical path length using Schlieren methods
G01N 21/77 - Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer
7.
SYSTEMS AND METHODS FOR VESICLE CARGO LABELING AND DETECTION
09 - Scientific and electric apparatus and instruments
Goods & Services
Biologic analysis and sample preparation and analysis equipment comprising computer hardware, downloadable software, and flat panel display screens for biologic formulation, buffer exchange and sample concentration for use in protein research and development, antibody research and development, viral vector research and development, vaccine research and development, biologic formulation, biologic development, and biologic production
9.
MICROFLUIDIC VISCOMETER AND ASSEMBLY, AND METHODS USING THE SAME
Provided herein are microfluidic viscometer assemblies and methods using the same, that include a microfluidic cartridge having microfluidic circuits that have channels adapted for viscosity determination without the need of a control fluid or oil. The viscometer assemblies also include an image recording system and a pressure control unit. In some embodiments, a temperature control unit is included as well. During methods using the viscometers provided herein, microfluidic cartridges can be loaded and removed from a viscometer, and disposed of.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
G01N 11/06 - Investigating flow properties of materials, e.g. viscosity or plasticityAnalysing materials by determining flow properties by measuring flow of the material through a restricted passage, e.g. tube, aperture by timing the outflow of a known quantity
10.
COMPOSITIONS, SYSTEMS, AND METHODS FOR ENHANCED LABEL-FREE AND FLUORESCENCE - BASED DETECTION OF NANOPARTICLES
Presented herein are compositions, systems, and methods related to optical substrates that simultaneous (1) enhance a fluorescence signal emitted by a fluorophore and (2) enhance “contrast” signal that comprises scattered signal intensity over substrate reflectivity at a non-fluorescent wavelength. In certain embodiments, the optical substrate comprises a thin, transparent, dielectric layer. In alternative embodiments, the optical substrate comprises a stack of thin, transparent dielectric layers, for example, that is designed for both specific scattering enhancement and fluorescence enhancement.
Described is a microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors. The well-plate includes a plurality of fluidic traps, a plurality of hydrophilic capillary constriction channels and a plurality of bypass channels. Each of the plurality of bypass channels is associated with one of the plurality of fluidic traps, each of the plurality of hydrophilic capillary constriction channels is associated with one of the plurality of fluidic traps, and each of the plurality of fluidic traps is associated with one of the plurality of bypass channels and one of the plurality of hydrophilic capillary constriction channels. The well-plate further includes an inlet, an outlet, and a main channel with a plurality of portions that connects the inlet to the plurality of fluidic traps, associated hydrophilic capillary constriction channels and associated bypass channels, and the outlet.
Various embodiments of fluidic devices and methods of the present teaching can provide precision on-device loading of fluidic samples, and merging, mixing, and splitting of the fluidic samples, in illustrative embodiments as droplets, using pressures that can be provided by standard laboratory liquid handling equipment. Various embodiments of fluidic devices of the present teachings can provide on-device manipulation of accurate and precise fluidic volumes at the picoliter to nanoliter scale for each steps from fluidic sample loading to fluidic sample splitting. Various embodiments of fluidic elements of the present teachings, for example, but not limited by, various embodiments of fluidic traps of the present teachings, can have a constrained and measurable geometry, allowing for accurate and precise tuning of each fluidic sample volume throughout the on-device liquid handling process.
Systems and methods for exchanging buffer solutions are disclosed. In accordance with some embodiments, the methods and systems for buffer exchange may be automated and/or the methods and systems may include mixing during filtering operations.
This disclosure provides fluidic devices and methods for performing a bioassay, for example bioassays performed on zebrafish. The disclosure provides various fluidic devices for performing a bioassay that include a sample chamber in fluid communication with an air valve; and a bioassay channel that can include a first bioassay region, for example for studying zebrafish in early stages of development and a second bioassay region, for studying zebrafish in later stages of development. The first bioassay region and second bioassay region can be defined using pillars, such as a first and second array of pillars. The fluidic device can have additional structures that are provided herein. Also provided herein are sample loading manifold devices for loading zebrafish embryos into fluidic devices and reagent delivery manifold devices for delivering reagents to fluidic devices. Furthermore, methods using any or all of the devices are provided.
The invention relates to a liquid cell (1) for the microscopic image capture and Raman spectroscopic material analysis of a particle suspension in a reflected light microscope, having at least the following components: a measuring chamber (2) which has a base (3), a measuring window (5) opposite the base (3), and a seal (6), wherein the base (3) has a planar design at least in one region of the support of the seal (6), and the base (3) has a reflective surface (4) which is provided such that Raman excitation light incident through the measuring window (5) is reflected on the reflective surface (4) in a directed manner such that the background signal in a Raman measurement is reduced and the Raman signal of a particle in a suspension is increased. The invention further relates to a microscope which has such a liquid cell.
The present disclosure provides fluidic devices that in some embodiments have passive air control valves and in some instances, high resistance air valve constriction channels, or channel dimensions and compositions that provide effective capillary pressure ratios, that can be used to fill reaction wells and/or manipulate fluids in reaction wells. Also provided are fluidic systems containing fluidic devices adjoined to one another, methods for operating the fluidic devices, and methods for manipulating fluids using the fluidic devices. Methods for use of the fluidic devices in performing immunoassays, nucleic acid detection, other assay systems, including but not limited to point of care applications are also provided.
Parallel reactor systems for synthesizing materials are disclosed. The reactor systems may include at least two reaction vessels and may be suitable for synthesizing materials produced from corrosive reagents, for example, Ziegler-Natta catalysts. Antechambers may be provided above the reaction vessels to help purge vapors produced by the corrosive reagents. Methods for preparing materials by use of such parallel reactor systems are also disclosed.
Sample plates and methods for exchanging buffer solutions are disclosed herein. The sample plates and methods may be used with automated buffer exchange systems where high pressures, for example, pressures of at least about 30 psig, are applied across a filtering membrane. Methods for manufacturing the sample plates are further disclosed.
Sample plates and methods for exchanging buffer solutions are disclosed herein. The sample plates and methods may be used with automated buffer exchange systems where high pressures, for example, pressures of at least about 30 psig, are applied across a filtering membrane. Methods for manufacturing the sample plates are further disclosed.
09 - Scientific and electric apparatus and instruments
Goods & Services
Protein analysis and sample preparation equipment comprised of computer, hardware, software and display screens for protein formulation, buffer exchange and sample concentration for use in, protein research, antibody research, formulation development, biologic development, and biologic production
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific equipment, devices, and tools, namely, computer software and computer database platforms for scientific research, testing, and data generation to enable users to design, execute, analyze, and report experimental results
09 - Scientific and electric apparatus and instruments
Goods & Services
Protein, nucleic acid and particle analysis equipment comprised of computer, computer hardware, software, and display screens for protein and nucleic acid detection and quantitative analysis for use in genomics research, ligand binding research, protein research, antibody research, biologic development, and biologic production; Protein and particle analysis equipment comprised of computer, computer hardware, software, and display screens for particle and protein detection and quantitative analysis for use in particle analysis, protein research, antibody research, biologic development, and biologic production
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific equipment, devices, and tools, namely, computer software and computer database platforms for scientific research, testing, and data generation to enable users to design, execute, analyze, and report experimental results
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific equipment, devices, and tools, namely, computer, software, hardware, display screens and computer database platforms for scientific research, testing, and data generation to enable users to design, execute, analyze, and report experimental results.
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific measuring equipment and apparatus, namely, microscopes and spectroscopes, for particle identification and analysis for use in life science and pharmaceutical research, development, and quality control; computer software for particle identification and analysis for use in life science and pharmaceutical research, development, and quality control
09 - Scientific and electric apparatus and instruments
Goods & Services
Scientific measuring equipment and apparatus, namely, microscopes and spectroscopes, for particle identification and analysis and silicone detection for use in life science and pharmaceutical research, development, and quality control; computer software for particle identification and analysis for use in life science and pharmaceutical research, development, and quality control
27.
SYSTEMS, APPARATUSES, AND METHODS FOR ASSESSMENT OF LONG TERM STABILITY OF SAMPLES
A method includes receiving a sample. The method also includes applying a denaturing agent to a first portion of the sample, and detecting, in response to the application of the denaturing agent, a first measure from the first portion of the sample. The method also includes modifying the temperature of a second portion of the sample and detecting, in response to the modifying the temperature of the second portion of the sample, a second measure from the second portion of the sample. The method also includes computing thermodynamic information for the sample based on the indication of the first measure, and computing kinetic information for the sample based on the indication of the second measure. The method also includes computing, based on the thermodynamic information and the kinetic information, an indication of temporal stability of the protein component of the sample.
Disclosed herein is a system, particularly a microscope system, for confocal Raman-spectroscopic measurements. The system is configured to detect minute sample amounts of microbes in a sample chamber with a lid, wherein the system is configured such that a comparably large working distance between the objective lens and the sample is sustained such that a measurement can be performed through the lid of the sample chamber. This allows for cultivating and measuring the sample in the same container.
Systems and methods for exchanging buffer solutions are disclosed. In accordance with some embodiments, the methods and systems for buffer exchange may be automated and/or the methods and systems may include mixing during filtering operations.
09 - Scientific and electric apparatus and instruments
Goods & Services
Protein analysis equipment comprised of computer, hardware, software and display screens for protein detection and quantitative analysis for use in particle analysis, protein research, antibody research, biologic development, and biologic production
31.
Methods for sampling from non-atmospheric vessels in a parallel reactor system
Methods for sampling reactor contents in parallel reactor systems are disclosed. The methods may be used to sample reactor contents in non-atmospheric (e.g., pressurized) reaction vessels.
The present invention relates to, among other things, methods and systems for recognizing and characterizing protein aggregation processes at the earliest possible time and use of such new methods and systems for (1) the identification and selection of protein formulations that minimize aggregation and extend long-term stability and (2) the identification of protein variants with the lowest tendency to aggregate.
Systems and methods for exchanging buffer solutions are disclosed. In accordance with some embodiments, the methods and systems for buffer exchange may be automated and/or the methods and systems may include mixing during filtering operations.
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
A system and method for creating a buffer solution having a desired pH value is disclosed. The method uses two known buffer solutions, each with predetermined pH values, and determines a mathematical relationship which defines the amount of each known buffer solution needed to create the buffer solution with the desired pH. This method can then be used to create one or more denaturation graphs, which demonstrate the stability of a protein at a given pH level.
Parallel reactor systems for synthesizing materials are disclosed. The reactor systems may be suitable for synthesizing materials produced from corrosive reagents. Methods for preparing materials by use of such parallel reactor systems are also disclosed.
09 - Scientific and electric apparatus and instruments
Goods & Services
Biologic, protein, and particle analysis equipment comprised of computer, hardware, software, and display screens for protein detection and quantitative analysis for use in ligand binding research, protein research, antibody research, biologic development, and biologic production; biologic, protein, and particle analysis equipment comprised of computer, hardware, software, and display screens for particle and protein detection and quantitative analysis for use in particle analysis, protein research, antibody research, biologic development, and biologic production
09 - Scientific and electric apparatus and instruments
Goods & Services
Biologic, protein, and particle analysis equipment comprised of computer, hardware, software, and display screens for protein detection and quantitative analysis for use in ligand binding research, protein research, antibody research, biologic development, and biologic production; biologic, protein, and particle analysis equipment comprised of computer, hardware, software, and display screens for particle and protein detection and quantitative analysis for use in particle analysis, protein research, antibody research, biologic development, and biologic production
38.
DNA and/or RNA determination from UV-VIS spectrophotometer data
A method for analyzing UV-VIS spectrophotometer data of a sample comprising at DNA and/or RNA is described. The method comprises receiving UV-VIS spectrophotometer data, fitting the UV-VIS spectrometer data taking into account at least one spectrum representative for a base pair being any of more of adenine-thymine (AT) or guanine-cytosine (GC) or adenine-uracil, and deriving from the fitting a quantification of an amount of DNA and/or RNA.
G01N 21/33 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
39.
Liquid dispensing for high-throughput experimentation
Methods, systems, and apparatus, including computer program products and implementing techniques for mixing liquid components. Quantities of two or more liquid components are transferred from pressurized source reservoirs to one or more destination locations by means of a fluid outlet that includes a dispense valve, and the two or more liquid components are mixed in the destination locations to create a plurality of fluid mixtures. Two or more dispensing technologies can be combined to provide for increased efficiency in the dispensing of high volume liquid components. The amounts of liquid components being dispensed can be monitored during the dispensing to provide feedback control of the dispensing.
A unit is provided comprising an array (2) of sample containers (1), said containers, being connected together and arranged in a planar configuration, each container having multiple optically transparent windows arranged such that the sample contained therein can be interrogated using simultaneous multiple optical analytical techniques, the array of containers being configured so as to allow optical access to the windows of each container in the array. Also provided is an apparatus comprising such a unit, a system comprising a combination of such an apparatus and unit and a method of analyzing multiple samples by introducing each individual sample into an individual container of such an apparatus, illuminating the samples and detecting and analyzing light emerging therefrom.
A system and method for determining the dissociation constant for a particular ligand is disclosed. In accordance with certain embodiments, the method creates a chemical denaturation curve of a protein in the absence of the ligand. A particular point is selected from this curve, such as the point at which 90% of the protein is unfolded. The molarity of chemical denaturant is determined for this selected point. A one point test is then performed for the protein with a predetermined concentration of the particular ligand. The fraction of protein which is unfolded at this point is then used to determine the dissociation constant for the ligand. This constant is used to quickly determine whether a particular ligard is well suited to be considered a potential drug candidate against that protein target.
A system for automatically creating a denaturation curve is disclosed. In accordance with certain embodiments, a movement system including a unit having a plurality of cannulas is used. The cannulas are in fluid communication with a fluid system, which allows the cannulas to draw in and dispense fluid. A measurement system is included which draws fluid from a well into a detector to determine a characteristic of the fluid. A controller is used to control these systems and also to create a denaturation graph from the measured characteristics. In another embodiment, a plurality of formulations may be created using the system.
The invention is directed to a dispensing unit suitable to precisely dispense material in powdery and/or granular form. The dispensing device comprises an gate valve opening sealingly closed by a blend. A control unit, which is interconnected to a scaling device, controls the opening of the gate valve opening by the position of the blend. The precise amount of the material is adjusted in relation to the signal of the scaling device.
Methods, systems, and apparatus, including computer program products, implementing techniques for mixing liquid components. Quantities of two or more liquid components are transferred from pressurized source reservoirs to one or more destination locations by means of a fluid outlet that includes a dispense valve, and the two or more liquid components are mixed in the destination locations to create a plurality of fluid mixtures. Two or more dispensing technologies can be combined to provide for increased efficiency in the dispensing of high volume liquid components. The amounts of liquid components being dispensed can be monitored during the dispensing to provide feedback control of the dispensing.
Described is a parallel batch reactor for effecting chemical reactions. The parallel batch reactor includes a plurality of reactor vessels for receiving components of a reaction, an inlet port for receiving pressurized fluid, and a plurality of valves configured to transfer fluid from the inlet port to the reactor vessels and fluidically isolate one or more of the reactor vessels from at least one of the other reactor vessels. The reactor further includes a pressure monitoring system comprising an array of pressure sensors configured to sense pressure in the reactor vessels. Each of the pressure sensors is aligned with one of the plurality of reactor vessels and located external to the reactor vessels and fluid passageways in fluid communication with the reactor vessels.
A robotic system with a common software and hardware platform for integration of a variety of modular components is provided that can be set up for a given application and reconfigured to address changing needs. Modules attach to the robotic platform with standard interfaces in order to maximize the platform's flexibility. Robotic arms also have interchangeability such as variable pitched Z-racks, powder handling or other technologies. The standard interfaces allow third party developers to develop and integrate their own custom modules. One module included with the robotic platform in accord with this invention is a module that both weighs and images a sample.
G06F 19/00 - Digital computing or data processing equipment or methods, specially adapted for specific applications (specially adapted for specific functions G06F 17/00;data processing systems or methods specially adapted for administrative, commercial, financial, managerial, supervisory or forecasting purposes G06Q;healthcare informatics G16H)
G05B 19/04 - Programme control other than numerical control, i.e. in sequence controllers or logic controllers
Apparatus and methods for screening a library of materials. Materials are provided in a plurality of locations on a substrate. The locations include first regions that are sufficiently transparent to a first form of radiation to permit analysis of the portion of the sample material supported in the first region using a first analytical technique, and second regions that are sufficiently transparent to a second form of radiation to permit analysis of a portion of the sample supported in the second region using a second analytical technique, but are insufficiently transparent to the first form of radiation to permit analysis of the portion of the sample material supported in the second region using the first analytical technique. Sample materials are screened at one or more sample locations of the substrate using the first analytical technique in the first region and the second analytical technique in the second region.
09 - Scientific and electric apparatus and instruments
Goods & Services
COMPUTER SOFTWARE FOR COMPILING AND CATALOGING RESEARCH OR DEVELOPMENT EXPERIMENT DESIGN, DATA FILES AND RESULTS, FOR ASSEMBLING DATA, COLLECTIONS OF MATERIALS, AND FORMULATIONS FOR USE IN SCIENTIFIC AND ENGINEERING RESEARCH, AND FOR GENERATING DATA FILES DESCRIBING OR DEFINING COLLECTIONS OF MATERIALS OR THE PREPARATION OF MATERIALS FOR USE IN SCIENTIFIC AND ENGINEERING RESEARCH
49.
SAMPLE PLATES FOR BUFFER EXCHANGE AND METHODS OF MANUFACTURE
Sample plates and methods for exchanging buffer solutions are disclosed herein. The sample plates and methods may be used with automated buffer exchange systems where high pressures, for example, pressures of at least about 30 psig, are applied across a filtering membrane. Methods for manufacturing the sample plates are further disclosed.
Systems and methods for exchanging buffer solutions are disclosed. In accordance with some embodiments, the methods and systems for buffer exchange may be automated and/or the methods and systems may include mixing during filtering operations.
G01N 1/38 - Diluting, dispersing or mixing samples
G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor
G01N 35/02 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
