The present invention relates to a method of fractionating a sample comprising proteins, polypeptides and/or peptides, said method comprising (a) performing a first change of physicochemical conditions of said sample, wherein said first change establishes precipitating conditions for said proteins, polypeptides and/or peptides, but does not establish denaturing conditions for said proteins, polypeptides and/or peptides; and (b) performing one or both of the following (i) and (ii): (i) adding solid particulate matter to said sample; and (ii) performing said method in a vessel with a rough surface; wherein steps (a) and (b) can be effected concomitantly or in any order and yield a first fraction of proteins, polypeptides and/or peptides as a first precipitate on said particulate matter and/or on said inhomogeneous surface, and a second fraction of proteins, polypeptides and/or peptides remaining in a supernatant; and (c) subjecting said first precipitate to a second change of physicochemical conditions which are capable of solubilizing a part of said first precipitate, thereby obtaining a third fraction remaining precipitated and a fourth fraction in solution.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in industry and science; chemicals for use
in science; chemical substances for analyses in
laboratories, other than for medical or veterinary purposes;
industrial chemicals; chemical reagents for use in genetic
research. Research apparatus and instruments; scientific research and
laboratory apparatus, educational apparatus and simulators;
laboratory apparatus for scientific research; apparatus and
instruments for research use; apparatus and instruments for
scientific research in laboratories; laboratory devices for
detecting the presence of biological compounds; laboratory
devices for detecting the presence of chemical compounds;
diagnostic apparatus for research laboratory use. Rental of measuring apparatus; rental of laboratory
apparatus and instruments; rental of scientific equipment;
rental of laboratory robots for laboratory testing; rental
of laboratory robots for laboratory research and analysis;
scientific laboratory services; scientific and technological
services; scientific and industrial analysis and research
services; blood analysis services for scientific research;
providing scientific research services.
The present invention relates to a method of reducing the dynamic range of biomolecules in a biological fluid, said method comprising bringing said biological fluid into contact with fibers; wherein biomolecules as bound to said fibers are characterized in that said dynamic range is reduced as compared to said biological fluid.
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
B01J 20/10 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
09 - Scientific and electric apparatus and instruments
Goods & Services
Research apparatus and instruments; scientific research and laboratory apparatus, educational apparatus and simulators; laboratory apparatus for scientific research; apparatus and instruments for research use; apparatus and instruments for scientific research in laboratories; laboratory devices for detecting the presence of biological compounds; laboratory devices for detecting the presence of chemical compounds
The present invention relates to a method of operating a device, said device comprising an array of vessels, one or more coils in sufficient proximity of at least two of said vessels such that an electric current flowing through said coil(s) exposes the interior of said vessels to a magnetic field, said at least two vessels each containing at least one first permanent magnet, and a power source connected to said coil(s), said method comprising: (a) delivering a fluctuating or oscillating electric current to said coil(s) to trigger movement of the first permanent magnets; and (b) intermittently applying a magnetic pulse sufficient to render first permanent magnets in nearby vessels not magnetically aligned with each other.
The present invention relates to a method of processing or fractionating a sample comprising proteins, polypeptides and/or peptides, said method comprising (a) changing the physicochemical conditions of said sample; and (b) performing one or both of the following (i) and (ii): (i) adding solid particulate matter to said sample; and (ii) performing said method in a vessel with a rough surface; wherein steps (a) and (b) can be effected concomitantly or in any order; and wherein said processing or fractionating yields one or more first fractions of proteins, polypeptides and/or peptides as a precipitate on said particulate matter and/or said inhomogeneous surface, and a second fraction of proteins, polypeptides and/or peptides remaining in a supernatant.
The present invention relates to a method of fractionating a sample comprising proteins, polypeptides and/or peptides, said method comprising (a) performing a first change of physicochemical conditions of said sample, wherein said first change establishes precipitating conditions for said proteins, polypeptides and/or peptides, but does not establish denaturing conditions for said proteins, polypeptides and/or peptides; and (b) performing one or both of the following (i) and (ii): (i) adding solid particulate matter to said sample; and (ii) performing said method in a vessel with a rough surface; wherein steps (a) and (b) can be effected concomitantly or in any order and yield a first fraction of proteins, polypeptides and/or peptides as a first precipitate on said particulate matter and/or on said inhomogeneous surface, and a second fraction of proteins, polypeptides and/or peptides remaining in a supernatant; and (c) subjecting said first precipitate to a second change of physicochemical conditions which are capable of solubilizing a part of said first precipitate, thereby obtaining a third fraction remaining precipitated and a fourth fraction in solution.
This invention relates to a method of dispersing magnetic particles, said method comprising or consisting of: (a) in a vessel, combining at least one permanent magnet and said magnetic particles in a liquid phase; and (b) triggering a fluctuating or oscillating motion of said permanent magnet using a magnetic field; thereby dispersing said particles.
This invention provides a method of cleaving at least one covalent bond in at least one molecule, wherein said method comprises (a) colliding at least one magnetic body with said at least one molecule; and/or (b) triggering collision of at least one nonmagnetic particle with said at least one molecule by moving said at least one magnetic body. In embodiments, the molecule is in suspension or solution and (a) the solution or suspension is a liquid sample such as a buffer or a bodily fluid, and the molecule is a nucleic acid; (b) the solution or suspension is drinking water or pool water, and the molecule is a macromolecular contamination; (c) the solution or suspension is sewage, and the molecule is a biological macromolecule; (d) the solution or suspension is food or beverage, and the molecule is gluten; (e) the solution or suspension comprises a protein, polypeptide and/or peptide, proteinaceous raw material for growth media and/or proteinaceous raw material for a personal care product such as a shampoo, a conditioner or soap, and the molecule is said or a protein, polypeptide and/or peptide; (f) the solution or suspension comprises a macromolecular toxin, and the molecule is said macromolecular toxin; or (g) the solution or suspension comprises undesired enzymatic activity, and the molecule is the protein exhibiting said enzymatic activity.
The present invention provides a method of preparing a sample for an analytic procedure, said sample comprising at least one protein, polypeptide or peptide molecule, and said method comprising fragmenting said molecule using at least one moving magnetic body.
The present invention relates to a method of fractionating a variety of analytes bound to a matrix, said method comprising: (a) eluting said analytes with a plurality of eluents, wherein a first elution step is effected with an elution buffer comprising a first numbered eluent, and a subsequent (n-th) elution step is effected with an elution buffer comprising an n-th numbered eluent, wherein said n-th numbered eluent has a lower dipole moment and/or a higher hydrophobicity than said first numbered eluent.
The present invention relates to a solid, magnetized or magnetizable composition comprising (i) a core consisting of or comprising a ferromagnetic or ferrimagnetic material; and (ii) at least one surface moiety selected from (1) a binding moiety; (2) a biomolecule; and (3) a chemically reactive moiety; wherein said moiety is covalently bound to (a) said core; or (b) a layer which is directly or indirectly attached to said core. The invention furthermore provides methods of producing such compositions and uses thereof.
H01F 1/00 - Magnets or magnetic bodies characterised by the magnetic materials thereforSelection of materials for their magnetic properties
H01F 1/06 - Magnets or magnetic bodies characterised by the magnetic materials thereforSelection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials metals or alloys in the form of particles, e.g. powder
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
15.
MEANS AND METHODS OF OPERATING DEVICES WITH MULTIPLE MAGNETS
The present invention relates to a method of operating a device, said device comprising an array of vessels, one or more coils in sufficient proximity of at least two of said vessels such that an electric current flowing through said coil(s) exposes the interior of said vessels to a magnetic field, said at least two vessels each containing at least one first permanent magnet, and a power source connected to said coil(s), said method comprising: (a) delivering a fluctuating or oscillating electric current to said coil(s) to trigger movement of the first permanent magnets; and (b) intermittently applying a magnetic pulse sufficient to render first permanent magnets in nearby vessels not magnetically aligned with each other.
The present invention relates to a method of processing or fractionating a sample comprising proteins, polypeptides and/or peptides, said method comprising (a) changing the physicochemical conditions of said sample; and (b) performing one or both of the following (i) and (ii): (i) adding solid particulate matter to said sample; and (ii) performing said method in a vessel with a rough surface; wherein steps (a) and (b) can be effected concomitantly or in any order; and wherein said processing or fractionating yields one or more first fractions of proteins, polypeptides and/or peptides as a precipitate on said particulate matter and/or said inhomogeneous surface, and a second fraction of proteins, polypeptides and/or peptides remaining in a supernatant.
This invention relates to a method of dispersing magnetic particles, said method comprising or consisting of: (a) in a vessel, combining at least one permanent magnet and said magnetic particles in a liquid phase; and (b) triggering a fluctuating or oscillating motion of said permanent magnet using a magnetic field; thereby dispersing said particles.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical compositions and materials for use in science;
reagents for use in scientific apparatus for chemical or
biological analysis; diagnostic reagents and preparations,
except for medical or veterinary use; biochemical
preparations for scientific purposes; chemical compositions
sold as kits for use in laboratory analyses. Scientific apparatus and instruments; laboratory apparatus
and instruments; laboratory centrifuges; laboratory
pipettes; pipette racks for laboratory use; laboratory
robots; test tubes; laboratory swabs [laboratory
instruments]; flasks for laboratory use; laboratory filters;
apparatus for preparing laboratory samples of substances for
later analysis; laboratory instruments sold as kits for use
in laboratory apparatus. Rental of laboratory apparatus and instruments.
The present invention provides a method of preparing a sample for an analytic procedure, said sample comprising at least one protein, polypeptide or peptide molecule, and said method comprising fragmenting said molecule using at least one moving magnetic body.
This invention provides a method of cleaving at least one covalent bond in at least one molecule, wherein said method comprises (a) colliding at least one magnetic body with said at least one molecule; and/or (b) triggering collision of at least one non- magnetic particle with said at least one molecule by moving said at least one magnetic body. In embodiments, the molecule is in suspension or solution and (a) the solution or suspension is a liquid sample such as a buffer or a bodily fluid, and the molecule is a nucleic acid; (b) the solution or suspension is drinking water or pool water, and the molecule is a macromolecular contamination; (c) the solution or suspension is sewage, and the molecule is a biological macromolecule; (d) the solution or suspension is food or beverage, and the molecule is gluten; (e) the solution or suspension comprises a protein, polypeptide and/or peptide, proteinaceous raw material for growth media and/or proteinaceous raw material for a personal care product such as a shampoo, a conditioner or soap, and the molecule is said or a protein, polypeptide and/or peptide; (f) the solution or suspension comprises a macromolecular toxin, and the molecule is said macromolecular toxin; or (g) the solution or suspension comprises undesired enzymatic activity, and the molecule is the protein exhibiting said enzymatic activity.
The present invention relates to a method of fractionating a variety of analytes bound to a matrix, said method comprising: (a) eluting said analytes with a plurality of eluents, wherein a first elution step is effected with an elution buffer comprising a first numbered eluent, and a subsequent (n-th) elution step is effected with an elution buffer comprising an n-th numbered eluent, wherein said n-th numbered eluent has a lower dipole moment and/or a higher hydrophobicity than said first numbered eluent.
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemicals for use in science for the purpose of tissue sample preparation using paramagnetic particles; reagents for use in scientific apparatus for chemical or biological analysis for the purpose of tissue sample preparation using paramagnetic particles; diagnostic reagents and preparations, except for medical or veterinary use for the purpose of tissue sample preparation using paramagnetic particles; biochemical preparations for scientific purposes for the purpose of tissue sample preparation using paramagnetic particles; chemical compositions sold as kits for use in laboratory analyses for the purpose of tissue sample preparation using paramagnetic particles Scientific apparatus and instruments, namely, homogenizers, sample carriers, sample tubes, processing plates, sealing plates, carrier racks; laboratory apparatus and instruments, namely, homogenizers, carrier plates, sample tubes, carrier racks, processing plates and sealing plates; laboratory centrifuges; laboratory pipettes; pipette racks for laboratory use; laboratory robots; test tubes; laboratory swabs being laboratory instruments; flasks for laboratory use; laboratory filters; apparatus for preparing laboratory samples of substances for later analysis; laboratory instruments, namely, carrier racks, processing plates, sealing plates, sealing rollers, bar magnets, sample racks sold as kits for use in laboratory apparatus Rental of laboratory apparatus and instruments
The present invention relates to a device comprising or consisting of: (a) a vessel; (b) a magnet inside said vessel; (c) means to trigger movement of said magnet with acoustic to ultrasonic frequency, preferably ultrasonic frequencies.
The present invention relates to a device comprising or consisting of: (a) a vessel; (b) a magnet inside said vessel; (c) means to trigger movement of said magnet with acoustic to ultrasonic frequency, preferably ultrasonic frequencies.
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Scientific apparatus, instruments and equipment for research, examination and experimentation purposes, namely, apparatus, instruments and equipment for sample preparation in the field of protein research, mass spectrometry-based research, mass spectrometry based proteomics; automated laboratory equipment for diagnostic procedure, namely, computer hardware, diagnostic scanners, equipment for sample preparation in the field of protein research, mass spectrometry-based research, mass spectrometry based proteomics; laboratory apparatus, measuring apparatus and instruments for the use in medical and veterinary diagnostic processes, namely, liquid handling robot for sample preparation; automatic laboratory robotic workstations and components and accessories therefore for sample preparation; laboratory equipment, namely, spin columns for laboratory use; spin laboratory equipment, namely, column adapter rings for laboratory use; laboratory equipment, namely, reagent bottles sold empty for laboratory use; laboratory equipment, namely, reagent bottle racks for laboratory use; laboratory equipment, namely, rotors for laboratory centrifuges; laboratory equipment, namely, rotor adapters for laboratory centrifuges; laboratory equipment, namely, rotor adapter holders for laboratory centrifuges; laboratory equipment, namely, laboratory centrifuges, laboratory microcentrifuges and microinjectors; laboratory equipment, namely, centrifuge buckets for laboratory centrifuges; sensors, namely, optical sensors and ultrasonic sensors; laboratory apparatus, equipment and supplies, namely, spin-columns, spin-column based solid-phase extraction consumables; pipetting systems, namely, laboratory pipettes; laboratory equipment, namely, mechanical, electronic and automatic pipettes and dispensers for laboratory use; disposable laboratory articles, namely, spin-columns, spin-column based solid-phase extraction consumables, adapters, buffers, buffer tubes, pipette tips; computer programs, recorded, for diagnostic purposes for sample preparation, mass spectrometry based research, mass spectrometry based protein research, mass spectrometry based proteomics; downloadable computer software programs for diagnostic purposes for sample preparation, mass spectrometry based research, mass spectrometry based protein research, mass spectrometry based proteomics; laboratory mixing apparatus, namely, gas and liquid mixers for laboratory use; all the aforesaid goods not in relation to the field of simulation and animation Measuring apparatus and instruments, namely, sample preparation device for medical diagnostic uses; Measuring apparatus and instruments, namely, hematology analyzers for medical diagnostic uses, for use in medical and veterinary diagnostic processes for sample preparation, the modification and manipulation of proteins and polypeptides, and for the marking, separating, isolating, cleaning, duplicating, sequencing and analysis of biopolymers
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
42 - Scientific, technological and industrial services, research and design
Goods & Services
Scientific apparatus, instruments and equipment for research, examination and experimentation purposes; automated laboratory equipment for diagnostic procedures; laboratory apparatus, measuring apparatus and instruments for the use in medical and veterinary diagnostic processes; automatic laboratory robotic workstations and components and accessories therefore; spin columns for laboratory use; spin column adapter rings for laboratory use; reagent bottles sold empty for laboratory use; reagent bottle racks for laboratory use; rotors for laboratory centrifuges; rotor adapters for laboratory centrifuges; rotor adapter holders for laboratory centrifuges; centrifuges, microcentrifuges and microinjectors; centrifuge buckets for laboratory centrifuges; sensors, namely, optical sensors and ultrasonic sensors; laboratory apparatus, equipment and supplies; pipetting systems, namely, laboratory pipettes; mechanical, electronic and automatic pipettes and dispensers for laboratory use; disposable laboratory articles; computer programs, recorded, for diagnostic purposes; downloadable computer software programs for diagnostic purposes; mixing apparatus, namely, gas and liquid mixers for laboratory use; all the aforesaid goods included in class 9 for use only in the fields of chemistry, genetics, biology and biochemistry and not in relation to the field of simulation and animation. Measuring apparatus and instruments, namely, sample preparation device for medical diagnostic uses, hematology analyzers for medical diagnostic uses, for the use in medical and veterinary diagnostic processes for sample preparation, the modification and manipulation of proteins and polypeptides, and for the marking, separating, isolating, cleaning, duplicating, sequencing and analysis of biopolymers. Services related to scientific research and development, namely services in the field of research, development and diagnostics in the field of chemistry, genetics, biology and biochemistry services as well as diagnostic services in the field of DNA, RNA and protein detection and analysis, services in the field of forensics as well as design for others in the field of chemistry, genetics, biology and biochemistry, all as far as contained in this class; rental of science and technology equipment for use only in the fields of chemistry, genetics, biology and biochemistry; leasing of science and technology equipment for use only in the fields of chemistry, genetics, biology and biochemistry.
27.
HYDROPHOBICITY-BASED FLOW PREVENTION IN SAMPLE PREPARATION
The present invention relates to a cartridge comprising or consisting of, from top to bottom, the following elements: (a) an inlet; (b) a top volume; (c) at least one optional layer made of a first material; (d) adjacent to (b) or, if present, to (c), at least one layer of chromatographic material; (e) adjacent to (d) at least one layer made of first material; and (f) an outlet; wherein said first material is hydrophobic and porous.
B01D 15/22 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
42 - Scientific, technological and industrial services, research and design
Goods & Services
Diagnostic reagents, other than for medical use for sale in
kit form; chemical diagnostic reagents for industrial use;
chemicals for use in industry and science; chemicals for use
in the pharmaceutical industry; chemical reagents for use in
diagnostic tests, other than for medical or veterinary use;
diagnostic preparations for scientific purposes; diagnostic
preparations, other than for medical or veterinary purposes;
diagnostic preparations for scientific purposes; diagnostic
reagents for in-vitro use [other than medical]; diagnostic
reagents, other than for medical laboratory use; diagnostic
preparations for scientific purposes; diagnostic substances,
other than for medical use; diagnostic reagents for in vitro
use in biochemistry, clinical chemistry and microbiology;
reagents for use in scientific apparatus for chemical or
biological analysis. Chemico-pharmaceutical preparations; diagnostic chemical
reagents for medical use; diagnostic preparations;
diagnostic agents for medical use; diagnostic agents for
pharmaceutical use; reagents for analytical purposes [for
medical purposes]; reagents for in-vitro laboratory use [for
medical purposes]. Biochemistry research services; biological research;
provision of information relating to scientific research;
medical research; pharmaceutical research; scientific
research services; scientific and technological services.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
42 - Scientific, technological and industrial services, research and design
Goods & Services
Diagnostic reagents and preparations, except for medical or veterinary use, sold in kit form; chemicals for use in industry and science; chemical additives for use in the manufacture of pharmaceuticals; chemical reagents for use in diagnostic tests, other than for medical or veterinary use; diagnostic preparations for scientific purposes; diagnostic preparations, other than for medical or veterinary purposes; diagnostic preparations for scientific purposes; diagnostic reagents for in-vitro use for non-medical purposes, namely, for use in biochemistry, clinical chemistry and microbiology; diagnostic reagents for scientific laboratory use; diagnostic preparations for scientific purposes; diagnostic preparations other than for medical or veterinary purposes; diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; reagents for use in scientific apparatus for chemical or biological analysis Diagnostic chemical reagents for medical use; diagnostic preparations for medical or veterinary purposes; diagnostic agents for medical use; diagnostic agents for pharmaceutical use; reagents for medical diagnostic purposes; reagents for in-vitro laboratory use for medical purposes Biochemistry research services; biological research; provision of information relating to scientific research; medical research; pharmaceutical research; scientific research services; scientific and technological services, namely, research and development of new products for others in the field of biological research
The present invention relates to a method of fractionating a variety of analytes bound to a matrix, said method comprising: (a) eluting said analytes with a plurality of eluents, wherein a first elution step is effected with an elution buffer comprising a first numbered eluent, and a subsequent (n-th) elution step is effected with an elution buffer comprising an n-th numbered eluent, wherein said n-th numbered eluent has a lower dipole moment and/or a higher hydrophobicity than said first numbered eluent.
This invention provides a method of cleaving at least one covalent bond in at least one molecule, wherein said method comprises (a) colliding at least one magnetic body with said at least one molecule; and/or (b) triggering collision of at least one non- magnetic particle with said at least one molecule by moving said at least one magnetic body. In embodiments, the molecule is in suspension or solution and (a) the solution or suspension is a liquid sample such as a buffer or a bodily fluid, and the molecule is a nucleic acid; (b) the solution or suspension is drinking water or pool water, and the molecule is a macromolecular contamination; (c) the solution or suspension is sewage, and the molecule is a biological macromolecule; (d) the solution or suspension is food or beverage, and the molecule is gluten; (e) the solution or suspension comprises a protein, polypeptide and/or peptide, proteinaceous raw material for growth media and/or proteinaceous raw material for a personal care product such as a shampoo, a conditioner or soap, and the molecule is said or a protein, polypeptide and/or peptide; (f) the solution or suspension comprises a macromolecular toxin, and the molecule is said macromolecular toxin; or (g) the solution or suspension comprises undesired enzymatic activity, and the molecule is the protein exhibiting said enzymatic activity.
This invention relates to a method of dispersing magnetic particles, said method comprising or consisting of: (a) in a vessel, combining at least one permanent magnet and said magnetic particles in a liquid phase; and (b) triggering a fluctuating or oscillating motion of said permanent magnet using a magnetic field; thereby dispersing said particles.
The present invention relates to a method of processing or fractionating a sample comprising proteins, polypeptides and/or peptides, said method comprising (a) changing the physicochemical conditions of said sample; and (b) performing one or both of the following (i) and (ii): (i) adding solid particulate matter to said sample; and (ii) performing said method in a vessel with a rough surface; wherein steps (a) and (b) can be effected concomitantly or in any order; and wherein said processing or fractionating yields one or more first fractions of proteins, polypeptides and/or peptides as a precipitate on said particulate matter and/or said inhomogeneous surface, and a second fraction of proteins, polypeptides and/or peptides remaining in a supernatant.
The present invention relates to a device comprising or consisting of: (a) a vessel; (b) a magnet inside said vessel; (c) means to trigger movement of said magnet with acoustic to ultrasonic frequency, preferably ultrasonic frequencies.
The present invention provides a method of preparing a sample for an analytic procedure, said sample comprising at least one protein, polypeptide or peptide molecule, and said method comprising fragmenting said molecule using at least one moving magnetic body.
