The present disclosure relates to simple and efficient method for capture and bioconversion of carbon dioxide or biological reduction of carbon dioxide to high value products. Particularly, the present disclosure relates to methods for co-utilization of methane and carbon dioxide using methanotrophic bacteria with or without an optional microbe. The disclosure also relates to corresponding recombinant microbes, method of preparation of recombinant microbes, and systems thereof.
The present disclosure discloses a system (100) for fermentation based protein production. The system (100) includes a fermenter (1) to convert gaseous raw material into biomass broth. Further, the system includes a concentrator (2) fluidly connected to the fermenter (1). The concentrator separates water content from a concentrated cell slurry of the biomass broth received from the fermenter (1). The separated water content from the biomass broth in the concentrator (2) is channelized back into the fermenter (1). A dryer (4) is fluidly connected to the concentrator, to remove liquid content from the concentrated cell slurry to form a dried biomass, where exhaust gases from the fermenter is adaptably processed to dry the concentrated cell slurry in the dryer. Furthermore, the system includes a finishing unit (5) which is connected downstream of the dryer (4), adapted to receive and process the dried biomass into packaged protein product.
The present disclosure provides a method of purifying or preparing microbial protein concentrate from microbial cell mass. More particularly, the present disclosure provides a method of preparing a microbial protein concentrate, comprising: subjecting a microbial cell mass to a temperature of about 100°C to 210°C and a pressure of about 0.1 bar to 20 bar to obtain a treated microbial cell mass; and separating protein from the treated microbial cell mass to obtain microbial protein concentrate with reduced nucleic acid content and color & odor removed. Additionally, the microbial protein concentrate obtained by the present method has high crude protein content and improved functional properties.
Whole cell based biostimulant compositions and methods for improving agricultural productivity the compositions including: a microbial consortium having gammaproteobacterial methanotroph. As a result of these methanotrophs, the biostimulant composition enables plant performance improvement, utilization of methane, and facilitates improved nitrogen fixation in plants. The composition also helps in reducing the need of external chemical fertilizers for plant growth, development, performance and/or survival.
The present disclosure relates to protein hydrolysate based biostimulant composition derived from methanotrophic bacteria, and methods for enhancing agricultural productivity. In particular, the compositions disclosed herein comprise a protein-derived component in an amount of about 30% or less with respect to weight of the composition, wherein said protein-derived component is obtained from a methanotrophic bacterium. The present biostimulant composition finds applications in methods for improving plant performance along with methods for reducing the need of external chemical fertilizer-based inputs for plant growth.
The instant disclosure is in the field of biosciences, more particularly towards molecular and industrial biotechnology. The present disclosure relates to recombinant methanotrophic bacteria capable of synthesizing indigo from methane comprising a gene encoding enzyme for increasing concentration of indole and a gene encoding enzyme for converting the indole to indoxyl. The present disclosure also relates to a method of developing the recombinant methanotrophic bacteria, and a method of indigo biosynthesis by the recombinant methanotrophic bacteria in presence of a methane source.
The present disclosure relates to growth media composition. The disclosure further relates to a method of producing the biomass at higher concentration by employing a growth media composition. The disclosure further relates to a method of producing value added product employing growth media composition. The growth media composition of the present disclosure is homogenous in nature and is self-sterilized. The present disclosure provides for enhanced productivity of the biomass and the value added products, respectively employing gaseous substrate.
The disclosure relates to whole cell based biostimulant compositions and methods for improving agricultural productivity. In particular, the compositions disclosed herein comprise a microbial consortium having gammaproteobacterial methanotroph. As a result of these methanotrophs, the biostimulant composition enables plant performance improvement, utilization of methane, and facilitates improved nitrogen fixation in plants. The composition also helps in reducing the need of external chemical fertilizers for plant growth, development, performance and/or survival.
The present disclosure relates to protein hydrolysate based biostimulant composition derived from methanotrophic bacteria, and methods for enhancing agricultural productivity. In particular, the compositions disclosed herein comprise a protein-derived component in an amount of about 30% or less with respect to weight of the composition, wherein said protein-derived component is obtained from a methanotrophic bacterium. The present biostimulant composition finds applications in methods for improving plant performance along with methods for reducing the need of external chemical fertilizer-based inputs for plant growth.
The disclosure relates to whole cell based biostimulant compositions and methods for improving agricultural productivity. In particular, the compositions disclosed herein comprise a microbial consortium having gammaproteobacterial methanotroph. As a result of these methanotrophs, the biostimulant composition enables plant performance improvement, utilization of methane, and facilitates improved nitrogen fixation in plants. The composition also helps in reducing the need of external chemical fertilizers for plant growth, development, performance and/or survival.
The present disclosure relates to protein hydrolysate based biostimulant composition derived from methanotrophic bacteria, and methods for enhancing agricultural productivity. In particular, the compositions disclosed herein comprise a protein-derived component in an amount of about 30% or less with respect to weight of the composition, wherein said protein-derived component is obtained from a methanotrophic bacterium. The present biostimulant composition finds applications in methods for improving plant performance along with methods for reducing the need of external chemical fertilizer-based inputs for plant growth.
The instant disclosure is in the field of biosciences, more particularly towards molecular and industrial biotechnology. The present disclosure relates to recombinant methanotrophic bacteria capable of synthesizing indigo from methane, a method of developing said recombinant methanotrophic bacteria, and a method of indigo biosynthesis by the recombinant methanotrophic bacteria in presence of a methane source.
The present disclosure relates to growth media composition. The disclosure further relates to a method of producing the biomass at higher concentration by employing a growth media composition. The disclosure further relates to a method of producing value added product employing growth media composition. The growth media composition of the present disclosure is homogenous in nature and is self-sterilized. The present disclosure provides for enhanced productivity of the biomass and the value added products, respectively employing gaseous substrate.
The present disclosure is related to a process comprising steps of fermentation, separation and purification that can be carried out in a centralized facility with large scale fermentation or in a decentralized facility with smaller scales of fermentation to make value added products from substrate not limiting to gaseous substrates effectively. The fermentation involves fermentative production of a marketable product using reactors or fermentors which are optimized for influential parameters such as gaseous substrate, gas hold up, mass transfer coefficient, etc. The separation step involves an effective way of separating desired product from fermentation broth by employing filtration, precipitation or adsorption techniques thereby enabling easy transportation of the product in case of a decentralized facility. The final stage of the process, either in centralized or decentralized facility, includes the elution of retained product and further purification of the same using downstream processing techniques.
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
C12P 7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
The present disclosure is related to a process comprising steps of fermentation, separation and purification that can be carried out in a centralized facility with large scale fermentation or in a decentralized facility with smaller scales of fermentation to make value added products from substrate not limiting to gaseous substrates effectively. The fermentation involves fermentative production of a marketable product using reactors or fermentors which are optimized for influential parameters such as gaseous substrate, gas hold up, mass transfer coefficient, etc. The separation step involves an effective way of separating desired product from fermentation broth by employing filtration, precipitation or adsorption techniques thereby enabling easy transportation of the product in case of a decentralized facility. The final stage of the process, either in centralized or decentralized facility, includes the elution of retained product and further purification of the same using downstream processing techniques.
C12M 1/04 - Apparatus for enzymology or microbiology with gas introduction means
C12P 1/04 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using bacteria
The present disclosure provides for production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium. In one embodiment, the recombinant methanotrophic bacterium includes exogenous nucleic acid(s) or gene(s) encoding for specified enzymes. In a further embodiment, succinic acid producing capacity of the recombinant methanotrophic bacterium is increased above the basal level by overexpression or/and downregulation of selected gene(s). In another embodiment, a process of producing succinic acid using the recombinant methanotrophic bacterium is disclosed. The present invention successfully solves the problems in converting organic waste to a useful chemical thereby providing an environment-friendly and commercially viable solution for waste management.
The present disclosure provides for production of lactic acid from organic waste or biogas or methane using recombinant methanotrophic bacteria. In one embodiment, the recombinant methanotrophic bacterium includes a heterologous nucleic acid encoding for lactate dehydrogenase (ldh) enzyme. In a further embodiment, the capacity of the recombinant methanotrophic bacterium for producing lactic acid over basal level is increased by overexpression or/and down-regulation or deletion of specified genes. In another embodiment, a process for producing lactic acid from organic waste using the recombinant methanotrophic bacterium is disclosed. The present disclosure provides a cradle to cradle environment-friendly and commercially viable solution for managing organic waste.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
The present disclosure provides for production of lactic acid from organic waste or biogas or methane using recombinant methanotrophic bacteria. In one embodiment, the recombinant methanotrophic bacterium includes a heterologous nucleic acid encoding for lactate dehydrogenase (Idh) enzyme. In a further embodiment, the capacity of the recombinant methanotrophic bacterium for producing lactic acid over basal level is increased by overexpression or/and down-regulation or deletion of specified genes. In another embodiment, a process for producing lactic acid from organic waste using the recombinant methanotrophic bacterium is disclosed. The present disclosure provides a cradle to cradle environment- friendly and commercially viable solution for managing organic waste.
The present disclosure provides for production of succinic acid from organic waste or biogas or methane using recombinant methanotrophic bacterium. In one embodiment, the recombinant methanotrophic bacterium includes exogenous nucleic acid(s) or gene(s) encoding for specified enzymes. In a further embodiment, succinic acid producing capacity of the recombinant methanotrophic bacterium is increased above the basal level by overexpression or/and down-regulation of selected gene(s). In another embodiment, a process of producing succinic acid using the recombinant methanotrophic bacterium is disclosed. The present invention successfully solves the problems in converting organic waste to a useful chemical thereby providing an environment-friendly and commercially viable solution for waste management.
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