Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and glycerol, as dissolved in a physiological solution.
A01N 1/126 - Physiologically active agents, e.g. antioxidants or nutrients
A01N 1/128 - Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gelsChemically altering living parts, e.g. by cross-linking
A01N 1/162 - Temperature processes, e.g. following predefined temperature changes over time
2.
ADHESION PREVENTING MATERIAL FOR OPHTHALMIC APPLICATIONS, AND METHOD FOR PRODUCING SAME
In specific embodiments, provided is a material for preventing adhesion of membranes in an ophthalmic surgery for treatment of glaucoma or the like without using mitomycin-C or the like. Particularly provided is such material, which is easy to handle and has no risk of histolysis or infections. In a production method of a preferred embodiment, prepared are: aldehyde-modified dextran (first reactant) having weight-average molecular weight of 10,000-5,000,000 and introduced aldehyde group-per-anhydrous glucose unit (mol/AGU) of 0.4-0.7; and succinic anhydride-added poly-L-lysine (second reactant) having weight-average molecular weight of 1000-100,000 and having residual amino group ratio of 80-99%; then, they are mixed together so that aldehyde group-to-amino group molar ratio becomes 0.9-1.1 and are reacted in presence of water so that polymer concentration becomes 8-20%; and a hydrogel produced by the reaction, or a suspension before gelation, is freeze-dried to produce a porous sheet having a thickness of 0.1-0.8 mm.
Provided are a cold storage method and a cold storage liquid that are suitable for non-freezing refrigerated storage of human or animal stem cells such as iPS cells or embryos in a non-frozen state. According to an embodiment, the non-freezing refrigerated storage liquid is: a mixture liquid (HTMα, HTMx2c13), in which MEM-alpha (Minimum Essential Medium Eagle (MEM) Alpha Modification) is mixed with a UW solution (UW_sln; BELZER UW (registered trademark) COLD STORAGE SOLUTION), or a modified UW solution (polymer component being changed to PVA), are mixed in a ratio of about 1/1 to 1/2; or a storage liquid having a composition equivalent to the mixture liquid, especially in respect of concentrations of potassium ions and sodium ions. And, arousal period(s) of maintaining a temperature of 30° C. to 37° C. is inserted at each of two to five days, into a non-freezing refrigerated storage period, which is made at 2° C. to 8° C. for 2 to 10 days or 4 to 20 days.
Provided are a cold storage solution that is suitable for the cold storage of human or animal stem cells such as iPS cells or embryos in a non-frozen state, and a cold storage method. In a cold storage solution according to one specific embodiment, use is made of, as a base, a solution [HTM(-)] prepared by mixing a minimum essential medium eagle (MEM) alpha modification (MEM-alpha) with a UW solution [UW_sln; BELZER UW (registered trademark) COLD STORAGE SOLUTION] or a modified UW solution (in which the polymer component is changed to PVA) at a ratio of about 1/1 to 1/2, or a cold storage solution having an equivalent composition thereto, in particular, the potassium ion and sodium ion concentrations. It is particularly preferable to add thereto 10-300 μM of a first additive that is at least one selected from among deferoxamine (DFO), deferasirox (DFX), deferiprone (DFP) and ferrostatin-1 (FST), 0.5-8 mM of a second additive that is trolox or vitamin E or a water-soluble analogue/derivative thereof, and 10-300 μM of sorbitol.
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
Medical adhesives; preparations for preventing adhesion for
medical purposes. Medical apparatus and instruments for use in surgery;
medical apparatus and instruments for surgical tissue
adhesives; medical apparatus and instruments for preventing
surgical adhesions; surgical adhesion barriers for medical
use.
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
Medical adhesives in the nature of a mixture of two powder chemical preparations, forming gels upon hydration on tissue, for use as hemostats, sealants, adhesion prevention barriers, and wound dressing associated with endoscopic examinations, sold in powder form; Medical adhesives in the nature of two liquid chemical preparations materials, forming gels upon mixing the two liquid materials on tissue, for use as hemostats, sealants, adhesion prevention barriers, and wound dressing associated with endoscopic examinations, sold in liquid form Medical apparatus and instruments for use in surgery for tissue adhesives, sealants, adhesive prevent barriers, hemostats and wound dressing during endoscopic examinations; medical apparatus and instruments for applying surgical tissue adhesives for use in surgery; medical apparatus and instruments for preventing surgical adhesions for use in surgery; surgical adhesion barriers for medical use being medical apparatus and instruments for use in surgery
A sheet-form gastrointestinal anastomosis reinforcing material that is wrapped around the entire circumference of a gastrointestinal anastomosed site from the outside and affixed by adding physiological saline solution from the outside, wherein: the gastrointestinal anastomosis reinforcing material is formed from a biodegradable base sheet having an interconnecting porous structure, and an adhesive resin layer formed to be fixed thereon; and the adhesive resin layer is formed from a first reactant composed of an aldehydated glycan, and a second reactant composed of partially carboxylated polylysine, the adhesive resin layer including granular structures derived from a powder of the first reactant, and a connecting layer that is derived from the second reactant and that interconnects the granular structures over the entire surface of a sheet-form tissue adhesive reinforcing material and fixes the granular structures to the base sheet.
A non-freezing refrigerated storage liquid for stem cells such as iPS cells, according to an embodiment, comprises: potassium ion species in a range of 30 to 80 mmoL/L; sodium ion species in a range of 30 to 80 mmoL/L, wherein ion-based molar ratio of sodium ion species to potassium ion species (ratio ofNa+ to K+) is in a range of 0.5 to 1.3; trolox or its analog at a concentration of 1 to 8 mM; adenine or a salt or derivative thereof at a concentration of 0.1 to 4.2 mM; all of or all but one, two or three of essential amino acids, total content of which is 50 to 200 mg; and non-essential amino acids, total content of which is 50 to 200 mg/L.
In a specific embodiment, the present invention provides a material for preventing the adhesion of membranes in an ophthalmic surgery for the treatment of glaucoma or the like without using mitomycin-C or the like. In particular, the present invention provides a material as mentioned above, which is easy to handle and has no risk of the melting of tissues or infections. In a production method according to a preferred embodiment, aldehyde-modified dextran (a first reaction agent) having a weight average molecular weight of 10,000 to 5,000,000 and an introduced aldehyde group amount per anhydrous glucose unit (mol/AGU) of 0.4 to 0.7 and succinic anhydride-added poly-L-lysine (a second reaction agent) having a weight average molecular weight of 1000 to 100,000 and having a residual amination ratio of 80 to 99% are prepared, and then these substances are mixed together in such a manner that the (aldehyde group)/(amino group) reaction molar ration becomes 0.9 to 1.1 and are reacted in the presence of water in such a manner that the polymer concentration becomes 8 to 20%, and a hydrogel produced by the reaction or a suspension obtained before gelatinization is freeze-dried to produce a porous sheet having a thickness of 0.1 to 0.8 mm.
Provided are a cryopreservation liquid for bovine reproductive cells such as bovine sperms and a cryopreservation method thereof. Adopted is a cryopreservation liquid comprising: 0.3 to 0.9 w/w % of an amphoteric polyelectrolyte (an antifreeze polyamino acid), which is ε-poly-L-lysine having a number average molecular weight of 1,000 to 20,000 wherein 50 to 99 mol % of amino groups of the ε-poly-L-lysine are blocked as carboxylated by having been reacted with the succinic anhydride; and 2 to 4 w/w % of glycerol, as dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises steps of: primary diluting, in which bovine semen is diluted to 2.5 to 10 times with a physiological solution and kept at 2 to 8° C.; and secondary diluting, in which the bovine semen is diluted to 5 to 20 times while being kept at 2 to 8° C., by adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to a suspension obtained in the primary diluting.
A01N 1/126 - Physiologically active agents, e.g. antioxidants or nutrients
A01N 1/128 - Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gelsChemically altering living parts, e.g. by cross-linking
A01N 1/162 - Temperature processes, e.g. following predefined temperature changes over time
11.
TWO-REACTANT SHEET-SHAPED ADHESIVE/REINFORCEMENT FOR TISSUES
A sheet-shaped tissue adhesive/reinforcement includes a base sheet having biodegradability and a communicative porous structure, and an adhesive resin layer fixed and formed on the base sheet. The adhesive resin layer includes a first reactant made of an aldehyded glycan and a second reactant made of partially carboxylated polylysine, and has a molar ratio of 1 as a ratio of an aldehyde group of the first reactant to an amine group of the second reactant. The adhesive resin layer has a structure of granules derived from powder of the first reactant, and a connecting layer derived from the second reactant. The connecting layer connects the granules to each other and fixes each of the granules onto the base sheet, throughout the sheet-shaped tissue adhesive/reinforcement.
A61L 24/04 - Surgical adhesives or cementsAdhesives for colostomy devices containing macromolecular materials
A61L 24/00 - Surgical adhesives or cementsAdhesives for colostomy devices
A61K 38/36 - Blood coagulation or fibrinolysis factors
B05D 7/24 - Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials for applying particular liquids or other fluent materials
12.
Powder spray device and medical adhesive excellent in self-decomposability and adhesiveness
A powder spray device includes a funnel member, a first three-way joint, an air-current supply tube, a discharge tube, a vibration motor, a bypass air-current tube, and a switching mechanism. The first three-way joint has a first opening connected to an outlet at a lower end of the funnel member. The air-current supply tube and discharge tube are respectively connected to second and third openings of the first three-way joint. The vibration motor is fixed onto an outer surface of a funnel body of the funnel member. The bypass air-current tube branches off from the air-current supply tube and is connected to the discharge tube. The switching mechanism switches from and to standby state, in which compressed gas is sent only through the bypass air-current tube, to and from spray-coating state, in which compressed gas is sent out through the air-current supply tube, and also through the bypass air-current tube.
The present invention provides a sheet-form tissue-reinforcing material and a method for producing the same, wherein the sheet-form tissue-reinforcing material is configured as follows in order to achieve an excellent tissue-reinforcing effect and excellent handling properties without needing to use fibrinogen or clotting performance derived from a patient. This sheet-form tissue-reinforcing material includes a base-material sheet that is biodegradable and has a communicated porous structure, and an adhesive agent resin layer formed by being secured on the base-material sheet. The adhesive agent resin layer includes a first reactant including aldehydated glycan, and a second reactant including partial carboxylated polylysine, and has: a granular structure derived from a powder of the first reactant; and a connection layer derived from the second reactant, the connection layer being anchored to the base-material sheet and being mutually connected to the granular structure. A mixed powder of a two-reactant adhesive agent comprising the powder of the first reactant and a powder of the second reactant is used in order to produce the sheet-form tissue-reinforcing material. For example, the mixed powder is dispersed in ethanol having a water content of less than 1%, and a dispersion liquid thereof is applied to the base-material sheet and then dried.
The present invention is a cell transfer agent comprising a composite particle coated by a sugar chain polymer wherein the composite particle consisting of an apatite comprising phosphate, carbonic acid, and calcium.
Provided are a vehicle body assembling method and a vehicle body assembling apparatus which allow a simple configuration in the vicinity of the connecting portion between an upper jig and a lower jig and allow an increase in the efficiency of assembling work (welding work). A vehicle body assembling apparatus is equipped with a jig for supporting vehicle body components in a preassembled position, the jig comprising an upper jig and a lower jig which are connected to each other in at least two places. Each of the connection places is provided with a connecting means for fixing a three-dimensional coordinate position while allowing uniaxial turning. The vehicle body assembling apparatus is also equipped with a conveying means for conveying the upper jig which supports the vehicle body components, and reduces the load applied to the lower jig from the upper jig when connecting the upper jig to the lower jig.
A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In embodiments, ε-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In embodiments for foods or pharmaceuticals, the ε-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.
C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
A23L 3/375 - Freezing; Subsequent thawing; Cooling with addition of chemicals with direct contact between the food and the chemical, e.g. liquid N2, at cryogenic temperature
A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
17.
Composition for cryopreservation of cells and tissues
A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In embodiments, ε-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In embodiments for foods or pharmaceuticals, the ε-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.
A61K 47/34 - Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
A23L 3/375 - Freezing; Subsequent thawing; Cooling with addition of chemicals with direct contact between the food and the chemical, e.g. liquid N2, at cryogenic temperature
Medical-use two-part adhesive comprising; first part comprised of an aqueous solution or a powder of aldehyde-groups-introduced alpha-glucan; and second part comprised of an aqueous solution or a powder of amino-groups-containing polymer that is formed of a polymer chain of amino-group-containing units and has a molecular weight in a range of 1000 to 20,000; as well as medical-use hydrogel resin obtained by curing the adhesive. A mixture of the first and second parts, at a time of mixing them to form the hydrogel has pH in a range of 5.0 to 8.0. In a preferred embodiment, the amino-groups-containing polymer is epsilon-poly-L-lysine produced by microorganism or by enzyme.
[PROBLEMS] To provide a self-degradable adhesive for medical use which ensures sufficient adhesion even at a site with oozing of a large amount of blood or another body fluid, in particular, ensures adhesion without any problem even in the case where such a site is located vertically. To provide an adhesive for medical use which is excellent in the ability to cover a pinhole. [MEANS FOR SOLVING PROBLEMS] An aldehyde-groups-introduced α-glucan powder having a weight-average molecular weight of from 1,000 to 200,000 is employed as a first reactant, while an aqueous solution containing an amino groups-containing polymer that is composed of a polymer chain of amino group-containing units or a powder thereof is employed as a second reactaint. The weight-average molecular weight of the above-described amino groups-containing polymer is from 1,000 to 20,000. When the first and second reactants as described above are mixed together, the resultant mixture has a pH value of from 5.0 to 8.0.
