Suggested are preparations, comprising or consisting of (a) C-methyl flavonoids, a salt of C-methyl flavonoids and/or an extract containing C-methyl flavonoids, and (b) at least one inorganic salt or mineral substance.
The present invention relates to a method for engineering a protein in a host cell, comprising the following steps: identifying a protein of interest and introducing the coding sequence of the protein of interest into the genome of the host cell; screening for hotspots for amino acid mutations in the protein of interest; generating a set of specific guide RNAs and libraries of homologous recombination template that generate mutations at the desired sites within the protein coding region; introducing the guide RNA and the library of homologous recombination template into the host cell, thereby producing mutated protein coding regions; screening to select for cells that express the protein of interest with desired activity and/or property from the mutated protein coding regions, thereby providing an engineered protein.
A23G 3/50 - Sweetmeats, confectionery or marzipanProcesses for the preparation thereof characterised by shape, structure or physical form, e.g. products with supported structure
A23G 4/06 - Chewing gum characterised by the composition
1. Suggested is the use of compounds according to Formula (I) and/or Formula (II) as sweetener and/or sweetness enhancer and/or masking agent for the unpleasant taste of another sweetener.
A23G 3/50 - Sweetmeats, confectionery or marzipanProcesses for the preparation thereof characterised by shape, structure or physical form, e.g. products with supported structure
A23G 4/06 - Chewing gum characterised by the composition
Suggested is the use of at least one, preferably two, three, four or more compounds according to Formula (I) wherein R1 = H or OH R2 = H or Apiose R3 = H or Rhamnose as sweetener and/or sweetness enhancer and/or masking agent for the unpleasant taste of another sweetener.
A23G 3/50 - Sweetmeats, confectionery or marzipanProcesses for the preparation thereof characterised by shape, structure or physical form, e.g. products with supported structure
A23G 4/06 - Chewing gum characterised by the composition
The invention relates to a seasoning mixture that can be obtained by: a) providing living fungal cells of a species of the subphylum Ustilaginomycotina or a species of the phylum Ascomycota in a fermentation medium; (b) adding a secondary feed flow as a substrate into the fermentation medium; (c) producing a fermented substrate by incubating the mixture obtained in step (b); (d) extracting the seasoning mixture by separating the fungal cells from the fermented substrate obtained in step (c); and (e) optionally drying the seasoning mixture from step (d).
Suggested is a method for recovering precious metals from secondary resources comprising or consisting of the following steps: (a) providing a source of solid waste material comprising precious metals in an amount of at least 0.0001% b.w.; (b) bringing said waste material into contact with heterotrophic micro-organisms capable of producing and releasing hydrocyanic acid; (c) adding a solvent or an aqueous nutrient solution capable of serving as a nutrient source for said micro-organisms to the mixture; (d) depleting said waste materials from the precious metals contained therein by complexation of the metals with said hydrocyanic acid released by said micro-organisms; (e) separating the depleted solid waste material from the liquid containing the metal-cyano complexes; (f) recovering the precious metals from their cyano-complexes in known manner.
Suggested are preparations, comprising or consisting of (a) C-methyl flavonoids, a salt of C-methyl flavonoids and/or an extract containing C-methyl flavonoids, and (b) at least one inorganic salt or mineral substance.
The present invention relates to a method for engineering a protein in a host cell, comprising the following steps: identifying a protein of interest and introducing the coding sequence of the protein of interest into the genome of the host cell; screening for hotspots for amino acid mutations in the protein of interest; generating a set of specific guide RNAs and libraries of homologous recombination template that generate mutations at the desired sites within the protein coding region; introducing the guide RNA and the library of homologous recombination template into the host cell, thereby producing mutated protein coding regions; screening to select for cells that express the protein of interest with desired activity and/or property from the mutated protein coding regions, thereby providing an engineered protein.
Suggested is a process for recovering precious metals from secondary sources encompassing the following steps: (a) providing a secondary source selected from the group consisting of flow ashes, incineration ashes or their concentrates; (b) subjecting said source of step (a) to a sieving process to obtain a fraction comprising particles having a diameter ranging from about 0.05 to about 1 mm; and (c) subjecting the fraction obtained in step (b) to any process capable of recovering said precious metals from the waste residue
The invention relates to a species of genus Pseudomonas identified as Pseudomonas BR11571, termed Candidatus Pseudomonas metallosolvens, having Accession Deposit Number DSM 32538.
Suggested is a method for recovering precious metals from secondary resources comprising or consisting of the following steps: (a) providing a source of solid waste material comprising precious metals in an amount of at least 0.0001 % b.w.; (b) bringing said waste material into contact with heterotrophic micro-organisms capable of producing and releasing hydrocyanic acid; (c) adding a solvent or an aqueous nutrient solution capable of serving as a nutrient source for said micro-organisms to the mixture; (d) depleting said waste materials from the precious metals contained therein by complexation of the metals with said hydrocyanic acid released by said micro-organisms; (e) separating the depleted solid waste material from the liquid containing the metal-cyano complexes; (f) recovering the precious metals from their cyano-complexes in known manner.
Suggested is a method for engineering and/or editing the genome of prokaryotes encompassing the following steps: (i) providing a culture of prokaryotic cells, (ii) preparing a vector comprising an expression system encompassing at least one programmable DNA-binding and-cleaving protein, (iii) introducing said vector into said prokaryotic cells to target a specific DNA sequence in the genome of said prokaryotic cells.
Suggested is a method for enhancing the expression of taste related receptor genes encompassing the following steps:(i) providing a culture of mammalian cells, the genome of said cells comprising at least one sweet receptor domain; (ii) designing at least one type of single-guide RNA (sgRNA), the 10 to 30 nt guide sequence of said sgRNA being complementary to stretches within the non-coding and/or putative regulatory region upstream of the translation start codon of at least one sweet receptor gene; (iii) preparing a vector comprising an expression cassette encompassing at least one optionally modified CRISPR-Cas9, preferably CRISPR-dCas9VP64, and at least one optionally modified sg-RNA optionally containing aptamer structures for binding activator proteins; (iv) transfecting said culture of mammalian cells with said vector to target the genome for the presence of a DNA sequence that is complementary to the 10 to 30 nt guide sequence of said sgRNA; and (v) measuring the transcriptional enhancement of the sweet receptor mRNA by quantitative RT-PCR.
The present invention relates to a method of producing yeast mutants, to yeast mutants and the use thereof. In order to provide yeasts which, at a given sugar content, produce a low ethanol content and a relatively high glycerol content in ethanolic fermentation and which are simultaneously obtainable rapidly, it is proposed in accordance with the invention that at least one yeast strain be contacted in a first mutagenesis step with a first mutagen and in a second mutagenesis step with a second mutagen, the first and second mutagens being different from one another and being selected from the following groups: nucleotide-alkylating agent, nucleotide-deaminating agent and UV radiation, and a first selection step being executed between the first and second mutagenesis steps and a second selection step being executed after the second mutagenesis step, in which the mutants that originate from the prior mutagenesis step in each case are exposed to a selection factor selected from the following groups: (a) hypertonic medium and (b) alcohol dehydrogenase inhibitor.
The present invention relates to an anti-dandruff composition comprising 1-acetoxychavicol acetate (I). Furthermore, the present invention relates to a method for preparing said anti-dandruff composition and the use of said anti-dandruff composition for treating or preventing Malassezia induced dandruff formation. Moreover, the present invention relates to a method for preparing an Alpinia galanga extract and an extract obtainable or obtained by said method as well as the use of this extract for treating Malassezia induced dandruff formation comprising applying an anti-dandruff composition comprising said extract.
A61K 8/97 - Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from algae, fungi, lichens or plantsCosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof, of undetermined constitution from derivatives thereof
The present invention relates to a composition comprising licoricidine and at least one component selected from the group consisting of glyasperin D, glyasperin C, gancaonin I and glycyrrhisoflavone, preferably wherein said composition is a Glycyrrhiza pallidiflora, Glycyrrhiza uralensis or Glycyrrhiza glabra extract, and to a method for preparing the same. Furthermore the present invention relates to pharmaceutical or cosmetic composition or a method for preparing the same, said pharmaceutical or cosmetic composition comprising licoricidine and at least one component selected from the group consisting of glyasperin D, glyasperin C, gancaonin I and glycyrrhisoflavone, wherein said composition is preferably a Glycyrrhiza pallidiflora, Glycyrrhiza uralensis or Glycyrrhiza glabra extract. Furthermore, the present invention relates to a pharmaceutical or cosmetic composition, as described above, for body and oral care, in particular for use as deodorant or for use in treating or preventing dental caries.
A61K 31/343 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having five-membered rings with one oxygen as the only ring hetero atom, e.g. isosorbide condensed with a carbocyclic ring, e.g. coumaran, bufuralol, befunolol, clobenfurol, amiodarone
A61K 31/353 - 3,4-Dihydrobenzopyrans, e.g. chroman, catechin
This invention relates to a cosmetic composition comprising or consisting of one or more compounds selected from the compounds of formulae (A) and (B) wherein ---- represents a single bond or double bond; R1 in formula (A) and R1 in formula (B) are selected from H, linear or branched C1 to C4 alkyl, linear or branched C1 to C4 alkenyl, linear or branched C1 to C4 alkinyl, and linear or branched C1 to C4 alkanoyl; R3 in formula (A) and R3 in formula (B) are selected from H, linear or branched C1 to C4 alkyl, linear or branched C1 to C4 alkenyl, and linear or branched C1 to C4 alkinyl; R2 and R4 in formula (A) are independently selected from H, OH and halogen, or R2 and R4 together are O to form an epoxid, wherein ---- represents a single bond in case R2 and R4 together are O; and R2 and R4 in formula (B) are defined as R2 and R4 in formula (A), respectively. Preferably, said cosmetic composition is for the cleaning of the skin. Also provided are means and methods for the prevention or reduction of biofilm formation on an intracorporeal device and/or for the removal of biofilm from an intracorporeal device, wherein said biofilm comprises or consists of bacteria of the genus Propionibacterium. Furthermore provided is an intracorporeal device which is coated and/or loaded with one or more compounds as defined above.
The present invention relates to a cosmetic composition for the cleaning of the skin, said composition comprising or consisting of one or more compounds of formula (I) wherein (formula II) represents a single bond or double bond; R1 and R2 are independently selected from H, linear or branched C1 to C4 alkyl, linear or branched C1 to C4 alkenyl, linear or branched C1 to C4 alkinyl, and linear or branched C1 to C4 alkanoyl; R4 is selected from H, linear or branched C1 to C4 alkyl, linear or branched C1 to C4 alkenyl, and linear or branched C1 to C4 alkinyl; and R3 and R5 are independently selected from H, OH and halogen, or R3 and R5 together are O to form an epoxid, wherein (formula) represents a single bond in case R3 and R5 together are O. Also provided are means and methods for the prevention or reduction of biofilm formation on an intracorporeal device and/or for the removal of biofilm from an intracorporeal device, wherein said biofilm comprises or consists of bacteria of the genus Propionibacterium. Furthermore provided is an intracorporeal device which is coated and/or loaded with one or more compounds as defined above.
The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.
The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a).
The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E. C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.
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