The invention comprises a method where extracellular space is sampled using high molecular weight cut off dialysis techniques in combination with proteomics using LC-MSMS after precipitation and digestion of samples.
The invention provides a biosensor for at least partial insertion in a fetal scalp, for monitoring an analyte in the body of the fetus, wherein the biosensor comprises a needle and a microdialysis element for sampling the analyte. The invention also provides a sensing system comprising the biosensor, configured to flow a liquid through the microdialysis element. The invention can be used for e.g. lactate sensing as well as releasing a pharmaceutical. Hence, the biosensor and/or sensing system may also be used for delivery of a pharmaceutical or nutrient to a fetus.
The invention provides a biosensor (10) comprising a biosensor unit (100) with an electrode (110), wherein the electrode (110) is rod-shaped, wherein the electrode (HO) further comprises a support (120) with an electrically conductive first layer (123) and an exclusion layer (126), wherein the electrically conductive first layer (123) is configured between the support (120) and the exclusion layer (126). The invention further provides a sensing system (1000) comprising such biosensor (10).
A61B 5/1486 - Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value using chemical or electrochemical methods, e.g. by polarographic means using enzyme electrodes, e.g. with immobilised oxidase
A61B 5/1473 - Measuring characteristics of blood in vivo, e.g. gas concentration or pH-value using chemical or electrochemical methods, e.g. by polarographic means invasive, e.g. introduced into the body by a catheter
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
b. an analysis process comprising subjecting the labeled analyte to MS, preferably LC-MS-MS.
Herein, preferably, the labeling process comprises reacting an analyte with a dialdehyde, wherein the dialdehyde carries a label bearing a charge, to provide a labeled analyte carrying the charge.
The present invention also provides a labeling method to provide a labeled analyte carrying a charge, wherein the labeling method comprises a labeling process comprising reacting an analyte with a dialdehyde, wherein the analyte comprises a primary amino group and wherein the dialdehyde carries a label bearing the charge.
The dialdehyde is preferably an aromatic dialdehyde, most preferably an aromatic 1,2- or 1,3-dicarboxaldehyde. The label preferably comprises a quaternary ammonium group and/or a quaternary phosphonium group.
The present invention also provides for a kit for labeling the analyte.
The invention provides a method for labeling an analyte comprising a primary amino group, the method comprising: (a.) a labeling process comprising reacting the analyte with a dialdehyde in the presence of a label, wherein the label bears a charge, and (b.) an analysis process comprising subjecting the labeled analyte to MS, preferably LC-MS-MS. Herein, preferably, the labeling process comprises reacting an analyte with a dialdehyde, wherein the dialdehyde carries a label bearing a charge, to provide a labeled analyte carrying the charge. The present invention also pro vides a labeling method to provide a labeled analyte carrying a charge, wherein the labeling method comprises a labeling process comprising reacting an analyte with a dialdehyde, wherein the analyte comprises a primary amino group and wherein the dialdeh yde carries a label bearing the charge. The dialdehyde is preferably an aromatic dialdehyde, most preferably an aromatic 1,2- or 1,3-dicarboxaldehyde. The label preferably comprises a quaternary ammonium group and/or a quaternary phosponium group. The present invention also provides for a kit for labeling the analyte.
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