Abstract

A magnetic response fiber material and a preparation method and an application thereof are provided, which relates to the field of oil-water separation materials. The preparation method includes: mixing a fiber-forming polymer, a primary solvent, a secondary solvent and magnetic nanoparticles to form a uniform spinning solution, where the fiber-forming polymer includes at least one of polyethylene, polypropylene and polymethylpentene; spinning the spinning solution by a spinning process to obtain the magnetic response fiber material. The prepared magnetic response fiber material has the advantages of high oil absorption speed, high oil absorption capacity and high separation efficiency. Magnetic nanoparticles have a high load, which can not only be driven by magnetic force to absorb oil floating on water surface, but also be driven to an underwater oil pollution position to absorb oil, and can be applied to water purification in oil-polluted areas that cannot be reached by manual processing.

IPC Classes  ?

• B01D 17/02 - Separation of non-miscible liquids
• B01D 39/16 - Other self-supporting filtering material of organic material, e.g. synthetic fibres
• B01D 39/20 - Other self-supporting filtering material of inorganic material, e.g. asbestos paper or metallic filtering material of non-woven wires
• C02F 1/28 - Treatment of water, waste water, or sewage by sorption
• C02F 1/40 - Devices for separating or removing fatty or oily substances or similar floating material
• D01D 1/02 - Preparation of spinning solutions
• D01D 5/00 - Formation of filaments, threads, or the like
• D01F 1/10 - Other agents for modifying properties
• D01F 6/04 - Monocomponent man-made filaments or the like of synthetic polymersManufacture thereof from homopolymers obtained by reactions only involving carbon-to-carbon unsaturated bonds from polyolefins
• D04H 1/728 - Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
• C02F 101/32 - Hydrocarbons, e.g. oil
• C02F 103/00 - Nature of the water, waste water, sewage or sludge to be treated

Abstract

The present disclosure provides a probiotic-containing sea cucumber peptide powder for improving an immunity and a preparation method thereof and belongs to the technical field of food processing. In the present disclosure, papain and a neutral protease are used in combination to allow step-by-step enzymatic hydrolysis, and Eurotium cristatum and/or Bacillus coagulans are/is used to allow fermentation, so as to prepare a probiotic-containing sea cucumber peptide powder without a fishy smell. Enzyme inactivation and decolorization are not required throughout the preparation method of the present disclosure. The probiotic-containing sea cucumber peptide powder prepared by the preparation method does not have a fishy smell, is light-yellow with a bright luster, and has a high live probiotic content. The probiotic-containing sea cucumber peptide powder is suitable for daily health care for people with a low immunity.

IPC Classes  ?

• A23J 1/04 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from fish or other sea animals
• A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics

Abstract

argR, patA, puuA, speED, speG, puuPargFydcSTUVpotFGHIplaPplaP. The present invention further proves that the chassis strain has a good application prospect in constructing engineering strains highly producing 1,4-butanediamine, and thus provides an excellent chassis strain for microbial fermentation production of 1,4-butanediamine.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/54 - Transferases (2)
• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12R 1/19 - Escherichia coli

Abstract

A method for preparing pseudouridine through whole-cell catalysis of recombinant Escherichia coli. The recombinant Escherichia coli is obtained by overexpressing pseudouridine-5-phosphoglycosidase, ribokinase, ribonucleoside hydrolase, cytosine deaminase and nucleoside permease, and not expressing pseudouracil transport protein in Escherichia coli E.coHMG1655; and whole-cell catalytic preparation of pseudouridine taking uridine or cytidine as a substrate is realized by using the recombinant Escherichia coli. A novel pseudouridine enzyme catalytic synthesis route is designed, and in optimal reaction conditions, the maximum yield of pseudouridine reaches 27.24 g/L, the conversion rate reaches 90.8%, the production efficiency is 1.135 g·h-1·L-1, and the pseudouridine yield and the production efficiency are high.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12P 19/30 - Nucleotides
• C12R 1/19 - Escherichia coli

Abstract

The present disclosure provides a preparation method of a yak hide-derived oligopeptide ferrous chelate with a high antioxidant activity. The preparation method includes preparing a yak hide-derived oligomeric collagen peptide and subjecting the yak hide-derived oligomeric collagen peptide as a protein source to chelation with an iron source in water. A pretreated yak hide is subjected to enzymatic hydrolysis under a pH value of 7 at 50° C. for 4 h with an amount of an enzyme added at 2% to obtain a yak skin-derived collagen with a molecular weight of less than 2 kDa; the chelation is conducted in a peptide-to-iron mass ratio of 1:1 to 5:1 with a peptide concentration of 1% to 5% at 30° C. to 70° C. for 20 min to 60 min under a pH value of 3 to 8; and an iron chelating capacity is 42.72 mg/g under optimal preparation conditions.

IPC Classes  ?

• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• C07K 1/12 - General processes for the preparation of peptides by hydrolysis
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C12N 9/64 - Proteinases derived from animal tissue, e.g. rennin

Abstract

A method for extracting pullulan polysaccharide from high-viscosity fermentation broth includes following steps: (1) removing cells from the fermentation broth; (2) removing proteins; (3) decolorizing by macroporous resin adsorption; (4) removing ions by ultrafiltration; and (5) drying, crushing and packaging. In the extraction method of the present application, by using natural polymer bioflocculant chitosan, the high-viscosity fermentation broth can be processed without dilution and addition of filter aids and organic solvents for alcohol precipitation, which reduces the pressure of subsequent decolorization, properly recycles the cell proteins, and avoids the potential hazard of the organic solvents. The method can obtain high-purity pullulan polysaccharide, improving the product yield and quality, reducing solid waste, reducing the production cost, and achieving a safe, efficient, continuous and automated production process.

IPC Classes  ?

• C08B 37/00 - Preparation of polysaccharides not provided for in groups Derivatives thereof

Abstract

Provided are a genetically engineered strain for producing pseudouridine and use thereof in the fermentation and production of pseudouridine. The strain performs heterologous overexpression of a pyrimidine nucleoside operon, overexpression of pseudouridine acid synthetase, overexpression of ribokinase, overexpression of ribonucleoside hydrolase, overexpression of uracil permease, and non-expression of pseudouridine transporter and pseudouridine kinase. The strain can increase the yield of pseudouridine.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12P 19/30 - Nucleotides
• C12R 1/19 - Escherichia coli

Abstract

An alkaline protease mutant having an improved enzyme activity obtained by means of iterative saturation mutagenesis using overlapping PCR technology, and a preparation therefor and the use thereof. The alkaline protease gene apr from Bacillus clausii is subjected to iterative saturation mutagenesis by using overlapping PCR technology, and the mutant, which has high levels of activity at a low temperature, is screened out and is efficiently expressed and prepared in Bacillus amyloliquefaciens, so that the problem whereby alkaline protease has a low level of activity in a low-temperature washing environment is solved.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 15/57 - Hydrolases (3) acting on peptide bonds (3.4)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12N 9/54 - Proteinases derived from bacteria bacteria being Bacillus
• C12R 1/07 - Bacillus
• C12R 1/10 - Bacillus licheniformis

Abstract

Disclosed in the present invention are a system and method for treating offshore oilfield production wastewater using an enhanced A/O process, and a use. The system comprises a sewage accumulation tank, an anoxic tank, an aerobic tank, and a sedimentation tank which are connected in sequence; a suspension filler made of porous polyethylene is provided in the anoxic tank; a filler is provided in the aerobic tank, the filler in the aerobic tank comprises a plurality of combined fillers, and the plurality of combined fillers are connected to each other by means of a central cable at intervals in an up-down direction; a stirrer is provided in the anoxic tank, and an aeration head is provided at the bottom of the aerobic tank. In the present invention, conventional A/O devices and processes are improved, the suspension filler made of porous polyethylene is provided in the anoxic tank, and the combined fillers are added into the aerobic tank, thereby increasing the sludge age of activated sludge, greatly increasing the enriched biomass of the activated sludge, enhancing the microbial activity, improving the microbial adaptability, increasing the removal rate of COD, NH4+-N, and TN, and achieving a better sewage treatment effect.

IPC Classes  ?

• C02F 3/10 - PackingsFillingsGrids
• C02F 3/30 - Aerobic and anaerobic processes
• C02F 103/10 - Nature of the water, waste water, sewage or sludge to be treated from quarries or from mining activities
• C02F 101/16 - Nitrogen compounds, e.g. ammonia
• C02F 101/30 - Organic compounds

Abstract

A fabric-based breathable and washable wearable sensor and a manufacturing method therefor. A nano conductive active material is adsorbed on the surface of an elastic fabric material by means of the interaction between the nano conductive active material and the elastic fabric material, and a hydrophobic layer is formed on the surface by means of hydrophobic modification.

IPC Classes  ?

• D06M 11/74 - Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereofSuch treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with carbon or graphiteTreating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereofSuch treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with carbidesTreating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereofSuch treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with graphitic acids or their salts
• D06M 10/02 - Physical treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents or magnetic fieldsPhysical treatment combined with treatment with chemical compounds or elements ultrasonic or sonicCorona discharge
• D06M 10/06 - Inorganic compounds or elements
• D06M 11/83 - Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereofSuch treatment combined with mechanical treatment, e.g. mercerising with metalsTreating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereofSuch treatment combined with mechanical treatment, e.g. mercerising with metal-generating compounds, e.g. metal carbonylsReduction of metal compounds on textiles
• D06M 15/564 - Polyureas, polyurethanes or other polymers having ureide or urethane linksPrecondensation products forming them
• B05D 1/02 - Processes for applying liquids or other fluent materials performed by spraying
• B05D 1/18 - Processes for applying liquids or other fluent materials performed by dipping
• B05D 1/26 - Processes for applying liquids or other fluent materials performed by applying the liquid or other fluent material from an outlet device in contact with, or almost in contact with, the surface
• B05D 1/28 - Processes for applying liquids or other fluent materials performed by transfer from the surfaces of elements carrying the liquid or other fluent material, e.g. brushes, pads, rollers
• B05D 5/12 - Processes for applying liquids or other fluent materials to surfaces to obtain special surface effects, finishes or structures to obtain a coating with specific electrical properties
• B05D 7/24 - Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials for applying particular liquids or other fluent materials

Abstract

A chemical alkali recovery treatment process for chemimechanical pulping waste liquid, comprising the following steps: adding a pretreatment reagent into the chemimechanical pulping waste liquid for a pretreatment reaction, and obtaining a first mixture system after the pretreatment reaction, the pretreatment reagent being at least one of a hydrophobic modifier, a free radical reaction initiator and a metal ion salting-out agent, and the pH value of the chemimechanical pulping waste liquid being 12-14; adding a flocculating agent into the first mixture system for a precipitation reaction, and obtaining a second mixture system after the precipitation reaction; and performing solid-liquid separation on the second mixture system to obtain a solid organic precipitate and an alkaline recovery solution, the pH value of the alkaline recovery solution being 11.8-13. The treatment process is simple in process and low in energy consumption, can efficiently recycle organic matter components and alkali liquor in the chemimechanical pulping waste liquid, and has high economic value and thus is suitable for industrial application and popularization.

IPC Classes  ?

• D21C 11/04 - Regeneration of pulp liquors of alkali lye
• D21C 11/00 - Regeneration of pulp liquors
• C02F 1/52 - Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
• C02F 101/34 - Organic compounds containing oxygen

Abstract

A printable transparent stress sensor, comprising a conductive layer and an elastic substrate, wherein the conductive layer is a transparent conductive layer (1) composed of conductive nanomaterials, the elastic substrate is a light-transmitting elastic layer with two or more layers and a modulus gradient structure, and the transparent conductive layer (1) is embedded in the elastic substrate and is connected to the highest-modulus layer.

IPC Classes  ?

• G01L 1/18 - Measuring force or stress, in general using properties of piezo-resistive materials, i.e. materials of which the ohmic resistance varies according to changes in magnitude or direction of force applied to the material
• G01L 1/00 - Measuring force or stress, in general
• C09D 1/00 - Coating compositions, e.g. paints, varnishes or lacquers, based on inorganic substances
• C09D 5/24 - Electrically-conducting paints
• C09D 175/04 - Polyurethanes
• C09D 183/04 - Polysiloxanes
• B41M 1/34 - Printing on other surfaces than ordinary paper on glass or ceramic surfaces
• B41M 5/00 - Duplicating or marking methodsSheet materials for use therein

Abstract

The present invention provides a biochar-based three-dimensional composite material and a method for remediating high-concentration chromium-contaminated soil. The biochar-based three-dimensional composite material is obtained by loading, oxidizing, and polymerizing biochar and m-phenylenediamine. The biochar-based three-dimensional composite material and a domesticated dominant strain are used together for remediating chromium-contaminated soil. According to the method, the defects that the microbial remediation period is long, the environmental adaptability is poor, physical adsorption of common biochar cannot fundamentally eliminate contaminants, and the like can be overcome. The synergistic effect of the two can enhance the actual remediation effect on the contaminated soil and shorten the remediation operation period. The biochar-based three-dimensional composite material of the present invention is simple in the preparation process, low in cost, environmentally friendly, free of secondary contamination, and significant in effect, thereby greatly increasing the reduction rate of Cr(VI), reducing the migration capacity and biological effectiveness of chromium, improving the soil stability, enhancing the soil fertility, and improving the diversity of soil microbial species.

IPC Classes  ?

• C12N 1/36 - Adaptation or attenuation of cells
• C12N 1/20 - BacteriaCulture media therefor
• C02F 101/22 - Chromium or chromium compounds, e.g. chromates
• B09C 1/08 - Reclamation of contaminated soil chemically
• B09C 1/10 - Reclamation of contaminated soil microbiologically or by using enzymes
• C02F 1/28 - Treatment of water, waste water, or sewage by sorption
• C12R 1/01 - Bacteria or actinomycetales

Abstract

A high-oxygen stress freshness preservation method suitable for fresh fruit storage: picking fruits, then performing precooling treatment, selecting a sample, placing the sample in a sealed box, introducing high-purity nitrogen to discharge other gases, introducing 50%-100% (w/w) of high-concentration oxygen for stress treatment for 20-30 min, opening the sealed box, standing for 20-30 min, and then performing static storage.

IPC Classes  ?

• A23B 7/148 - Preserving or ripening with chemicals not covered by group or in the form of gases, e.g. fumigationCompositions or apparatus therefor in a controlled atmosphere, e.g. partial vacuum, comprising only CO2, N2, O2 or H2O

Abstract

A tube plate welding device, comprising a first welding head (7), a second welding head (8), a device body and a visual system (9). The device body comprises a first sliding table (1), a second sliding table (2), a third sliding table (3), a fourth sliding table (4), a fifth sliding table (5) and a sixth sliding table (6), wherein the first sliding table, the fifth sliding table, the sixth sliding table and the first welding head driven thereby form a first welding channel; and the second sliding table, the third sliding table, the fourth sliding table and the second welding head driven thereby form a second welding channel. Further disclosed is a tube plate welding method. According to the device, one set of visual systems is used for detection, two sets of welding devices are controlled to conduct welding operations at the same time, a welding path is systematically planned, steel tubes are continuously welded, and two steel tubes can be welded at one time by means of the double channels, thus significantly improving the welding efficiency.

IPC Classes  ?

• B23K 37/00 - Auxiliary devices or processes, not specially adapted for a procedure covered by only one of the other main groups of this subclass
• B23K 101/06 - Tubes

Abstract

Preparation method of modified starch-lipid binary complexes is provided. The preparation method includes: subjecting native starch to chemical modification utilizing octenyl succinic anhydride; and preparing a starch suspension with a concentration of 7-20% by taking starch octenyl succinate and lipid as raw materials at a mass ratio of (10-200):1. The modified starch-lipid binary complexes can be efficiently prepared through an aqueous phase system. According to the preparation method, not only the complexing of starch with fatty acid or monoglyceride but also the complexing of starch with diglyceride can be effectively promoted during food processing. Compared with the traditional technologies, the starch-lipid complexes prepared based on the present disclosure are more efficient, better structured, and more potentially useful for enhancing food quality to improve human nutritional health.

IPC Classes  ?

• C08B 31/04 - Esters of organic acids
• A23L 29/219 - Chemically modified starchReaction or complexation products of starch with other chemicals

Abstract

The present invention belongs to the field of genetic engineering, and specifically provided are an acetic acid bacterium for improving the flavor of vinegar and a construction method therefor. The following scheme is used: a first scheme, wherein the acetic acid bacterium is obtained by means of the coordinated expression of tyrosine ammonia lyase using Acetobacter pasteurianus as a host cell and ethanolamine ammonia lyase with synergistic effects in Acetobacter pasteurianus as a promoter; or a second scheme, wherein the acetic acid bacterium is obtained by means of the coordinated expression of aspartate ammonia lyase using Acetobacter pasteurianus as a host cell and cis-aconitase in the tricarboxylic acid cycle of Acetobacter pasteurianus as a promoter. When tyrosine ammonia lyase is used for recombination, the tyrosine ammonia lyase is converted into p-coumaric acid, which is a phenolic compound and has an effect of promoting the improvement of vinegar flavor and the efficacy thereof. When aspartate ammonia lyase is used for recombination, aspartate ammonia lyase is converted into fumaric acid, and the fumaric acid participates in the TCA cycle, which can enhance an energy metabolism to generate ATP and reduce the damage of the acid to cells.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
• C12N 15/60 - Lyases (4)
• C12R 1/02 - Acetobacter

Abstract

33 in sequence, enabling the pyrrole monomer to form polypyrrole that coats the surface of the chitosan, placing the prepared chitosan/polypyrrole mixed solution in a refrigerator for refrigeration overnight; and adding the chitosan/polypyrrole mixed solution to a precipitant prepared from absolute ethyl alcohol and ethyl acetate until a black flocculent substance is precipitated, and centrifugally washing and freeze-drying the black flocculent substance to obtain the remediation agent. The remediation agent can be conveniently used for the remediation of Cr(VI)-contaminated soil, has a spongy microstructure and developed porosity, can further play a role in reduction while adsorbing Cr(VI), does not affect the reusability of the soil after remediation is completed, and even can increase the fertility of the soil to promote the growth of plants.

IPC Classes  ?

• C09K 17/32 - PrepolymersMacromolecular compounds of natural origin, e.g. cellulosic materials
• C09K 101/00 - Agricultural use

Abstract

Provided in the present invention are a new electrocatalytic membrane reactor and the use thereof in preparation of high-purity hydrogen. The electrocatalytic membrane reactor uses an H-shaped electrolytic cell. A cathode chamber and an anode chamber are spaced apart from each other by a diaphragm. A membrane electrode is taken as an anode, and an auxiliary electrode is taken as a cathode. A direct-current stabilized-voltage power supply provides a constant current. The flow of a reaction liquid is realized by a pump. According to the present invention, electrocatalysis is coupled with a separation function of a membrane; oxygen evolution reaction in an anode chamber is replaced by the electrochemical oxidation reaction of organic matter, thereby reducing the overpotential of the oxygen evolution reaction; and hydrogen evolution reaction takes place in a cathode chamber to prepare high-purity hydrogen, such that the produced hydrogen has high purity, the current density is large, the response is rapid, and the preparation can be easily combined with renewable energy sources and the energy consumption and cost of hydrogen production are thus low. More importantly, the membrane reactor can use waste water, which contains phenol, dye and other refractory organic matter, as a water source, such that the energy consumption and cost of water treatment can be reduced, and the overpotential and energy consumption of anode reaction and the cost of hydrogen can also be greatly reduced.

IPC Classes  ?

• C02F 1/461 - Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
• C25B 9/19 - Cells comprising dimensionally-stable non-movable electrodesAssemblies of constructional parts thereof with diaphragms
• C25B 11/04 - ElectrodesManufacture thereof not otherwise provided for characterised by the material
• C02F 101/34 - Organic compounds containing oxygen

Abstract

The disclosure provides a novel electrocatalytic membrane reactor and use thereof in preparation of high-purity hydrogen. The electrocatalytic membrane reactor adopts an H-shaped electrolytic tank in which a cathode chamber is isolated from an anode chamber through a diaphragm, a membrane electrode is used as an anode, an auxiliary electrode is used as a cathode, a direct-current regulated power supply supplies a constant current, and the flow of a reaction solution is realized through a pump. In the disclosure, electrocatalysis is coupled with a membrane separation function, an oxygen evolution reaction is replaced with an organic electrochemical oxidation reaction in the anode chamber so as to reduce the overpotential of the oxygen evolution reaction, and a hydrogen evolving reaction is performed in the cathode chamber to prepare high-purity hydrogen.

IPC Classes  ?

• C25B 9/23 - Cells comprising dimensionally-stable non-movable electrodesAssemblies of constructional parts thereof with diaphragms comprising ion-exchange membranes in or on which electrode material is embedded
• C02F 1/467 - Treatment of water, waste water, or sewage by electrochemical methods by electrolysis by electrochemical disinfection
• C02F 1/461 - Treatment of water, waste water, or sewage by electrochemical methods by electrolysis
• C25B 1/04 - Hydrogen or oxygen by electrolysis of water

Abstract

The present invention provides a process for extracting pullulan polysaccharide from high-viscosity fermentation broth. The process comprises the following steps: (1) removing thalli from the fermentation broth; (2) removing protein; (3) performing macroporous adsorption resin discoloration; (4) removing ions by means of ultrafiltration; and (5) drying, crushing and packaging. The extraction process in the present invention uses chitosan as a natural polymeric biogenic flocculant, does not need the high-viscosity fermentation broth to be diluted, does not require the addition of filter aids and organic solvents for alcohol precipitation, and reduces the pressure of subsequent decolorization. Moreover, bacterial protains can be properly recycled, thereby avoiding the potential hazard of the organic solvents. The method can obtain high-purity pullulan polysaccharide, improve the product yield and quality, reduce solid waste, reduce the production cost, and achieve a safe, efficient, continuous and automated production process.

IPC Classes  ?

• C08B 37/02 - DextranDerivatives thereof

Abstract

Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

IPC Classes  ?

• C12P 13/14 - Glutamic acidGlutamine
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

The present invention relates to the technical field of food fermentation, and provides a rational construction method and a use of a synthetic bacterial flora for vinegar fermentation. The synthetic bacterial flora construction method provided by the present invention is based on a macro transcriptome sequencing technology, and adopts for the first time a cluster analysis to construct the synthetic bacterial flora. Compared with an in-situ acetic acid fermentation process for vinegar, the similarity of active microbial communities is greater than or equal to 90%, the similarity of flora metabolic characteristics is greater than or equal to 80%, and the synthetic bacterial flora can be rationally constructed. The method can well improve the stability of vinegar fermentation, provides reference for the construction and analysis of bacterial flora for other traditional fermentation method as well as for repeatable flavor synthesis technologies, and has good scientific value and application value.

IPC Classes  ?

• G16B 30/10 - Sequence alignmentHomology search
• G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• G16B 50/00 - ICT programming tools or database systems specially adapted for bioinformatics

Abstract

Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.

IPC Classes  ?

• C12N 9/54 - Proteinases derived from bacteria bacteria being Bacillus
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/55 - Hydrolases (3)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Abstract

Bacillus subtilis is further integrated to the genome and strongly expressed to promote generation of histidine.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12P 13/24 - ProlineHydroxyprolineHistidine
• C07K 14/245 - Escherichia (G)

Abstract

Saccharomyces cerevisiae strain with high yield of ethyl acetate and low yield of higher alcohols provided by the present disclosure not only maintains excellent ethanol fermentation characteristics, but also reducing the production of higher alcohols which adversely affect the comfort after drinking, which is of great significance for a well-maintained and strengthened flavor characteristics of Chinese Baijiu, an improved and stabilized quality thereof, and even a reform in the fermentation process thereof.

IPC Classes  ?

• C12N 1/18 - Baker's yeastBrewer's yeast
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12R 1/865 - Saccharomyces cerevisiae

Abstract

Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

IPC Classes  ?

• C12P 13/14 - Glutamic acidGlutamine
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor

Abstract

Provided are a genetically engineered bacterium for producing L-sarcosine, a construction method therefor and the use thereof. The genetically engineered bacterium takes escherichia coli as a host, and is obtained by means of integrating the following steps on the genome thereof: single copying an imine reductase gene dpkA; single copying a citrate synthase gene gltA; knocking out a glyoxylate cycle-inhibiting gene iclR; knocking out a malate synthase gene aceB; single copying an isocitrate lyase gene aceA; single copying a membrane-bound transhydrogenase gene pntAB; knocking out 2-keto acid reductase gene ycdW; single copying a phosphoenolpyruvate carboxylase gene ppc; and knocking out a pyruvate kinase gene pykF. After systemic metabolic modification is performed, the genetically engineered bacterium can be used for synthesizing L-sarcosine by means of taking glucose and methylamine as the main raw materials. After fermentation is performed in a 5 L fermentation tank for 30 h, the yield of L-sarcosine can reach 10 g/L.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/53 - Oxidoreductases (1)
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/54 - Transferases (2)
• C12N 15/60 - Lyases (4)
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12P 13/04 - Alpha- or beta-amino acids
• C12R 1/19 - Escherichia coli

Abstract

Escherichia coli as a host and by integrating a single copy of imine reductase gene dpkA on its genome; singly copying citrate synthase gene gltA; knocking out glyoxylate cycle inhibitor gene iclR; knocking out malate synthase gene aceB; integrating a single copy of isocitrate lyase gene aceA; integrating a single copy of membrane-bound transhydrogenase gene pntAB; knocking out 2-ketate reductase gene ycdW; integrating a single copy of phosphoenolpyruvate carboxylase gene ppc; and knocking out pyruvate kinase gene pykF. After system metabolism transformation, the engineered strain can synthesize sarcosine with glucose and methylamine as main raw materials. The sarcosine titer can reach 10 g/L after fermentation for 30 h in a 5 L fermenter.

IPC Classes  ?

• C12P 13/00 - Preparation of nitrogen-containing organic compounds
• C12N 1/20 - BacteriaCulture media therefor
• C12N 9/88 - Lyases (4.)
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/06 - Oxidoreductases (1.), e.g. luciferase acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• C12N 9/10 - Transferases (2.)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12R 1/19 - Escherichia coli

Abstract

A wort thin film enhanced mass transfer boiling system, comprising a boiling precipitation tank pot body, a centrifugal film formation enhanced mass transfer device, a steam inlet pipeline system, a steam outlet pipeline system, a first variable-frequency material pump, a second variable-frequency material pump, a wort cooling system, and a CIP system. A boiling process using the system comprises the steps of feeding, heat preservation, heating to boiling, vacuum evaporation after boiling, vacuum flash evaporation, and the like.

IPC Classes  ?

• C12C 7/22 - Processes or apparatus specially adapted to save or recover energy

Abstract

The present invention relates to an alkaline protease mutant, a gene thereof, an engineering bacteria thereof, a preparation method therefor and the use thereof, which belong to the technical field of bioengineering. The preparation method of the present invention comprises: extracting genome DNA from Bacillus clausii; performing PCR amplification to obtain a wild-type alkaline protease gene sequence; performing mutation by means of error-prone PCR on the wild-type alkaline protease gene obtained by amplification; performing high-throughput screening to obtain multiple alkaline protease genes having a high activity; performing DNA shuffling on the alkaline protease genes having a high activity; and screening same to obtain eight alkaline protease mutant genes having a higher activity.

IPC Classes  ?

• C12N 9/54 - Proteinases derived from bacteria bacteria being Bacillus
• C12N 15/57 - Hydrolases (3) acting on peptide bonds (3.4)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12R 1/125 - Bacillus subtilis
• C12R 1/10 - Bacillus licheniformis
• C12R 1/07 - Bacillus

Abstract

Disclosed in the present invention are a preparation method for a porous spinning composite material, and an application thereof in lithium extraction. The method comprises the following steps: (1) mixing a high molecular polymer with an organic solvent, heating and stirring, fully dissolving the polymer and the organic solvent, and standing for defoaming; (2) adding lithium ion sieve powder and a water-soluble porogen into the mixed solution, and fully stirring and uniformly mixing; (3) spraying the mixed solution into a coagulating bath by means of a wet spinning device for phase inversion molding; (4) drafting and washing, and drying and curing to obtain a porous spinning composite adsorption material. The obtained porous spinning composite adsorption material is applied to extraction of lithium in liquid lithium ore. In the present invention, the problems of poor fluidity and cycling stability of a powder material are effectively solved, and the adsorption rate and adsorption capacity of the powder material are greatly improved relative to granular and rod-shaped adsorption materials. Therefore, the method has good application prospects in development and utilization of lithium resources in salt lake brine, underground brine, geothermal water and the like.

IPC Classes  ?

• B01J 20/26 - Synthetic macromolecular compounds
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• B01J 20/30 - Processes for preparing, regenerating or reactivating
• C22B 26/12 - Obtaining lithium
• C22B 3/24 - Treatment or purification of solutions, e.g. obtained by leaching by physical processes, e.g. by filtration, by magnetic means by adsorption on solid substances, e.g. by extraction with solid resins

Abstract

E. coli chromosome, using the promoter of xylose transporter coding gene xylF to control the RNA polymerase from T7 bacteriophage, reconstructing a synthesis pathway of ectoine and constructing a plasmid-free system, and enhancing the expression of target genes by a strong promoter T7; the yield of ectoine reached 12-16 g/L after 20-28 h fermentation in shake flask, and reached 35-50 g/L after 24-40 h fermentation in a 5 L fermentor.

IPC Classes  ?

• C12P 17/12 - Nitrogen as only ring hetero atom containing a six-membered hetero ring
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/10 - Transferases (2.)
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 9/88 - Lyases (4.)

Abstract

Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.

IPC Classes  ?

• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Abstract

Disclosed is a non-sintering method for preparing an artificial cobblestone from dredged soil, comprising the steps of: (1) preparing raw materials; (2) proportioning four types of materials; (3) preparing high-strength non-sintering ceramsite; (4) preparing a cobblestone core; (5) preparing a primary product of the cobblestone; (6) polishing; (7) curing; and (8) forming a finished product. In the method, the dredged soil is used as the raw material to prepare the artificial cobblestone with a core-shell structure, so that an application range of dredged soil recycling utilization can be widened, and a method for preparing artificial cobblestones is provided. By employing the non-sintering method for preparation, the energy consumption for production is low, and a decorative effect of the cobblestone can be achieved.

IPC Classes  ?

• C04B 38/00 - Porous mortars, concrete, artificial stone or ceramic warePreparation thereof

Abstract

A superhydrophobic polypropylene porous film, including a polypropylene porous film substrate, titanium dioxide layers and a surface modifier layer, is disclosed. The titanium dioxide layers are deposited on the surface of the polypropylene porous film substrate by atomic deposition technology; a surface modifier is coated on the titanium dioxide layers; hydrophobic bonds are formed between the titanium dioxide layers and the surface modifier layer; the superhydrophobic polypropylene porous film has a water contact angle greater than 150 degrees, a rolling angle less than 10 degrees, an aperture of 0.1-0.4 μm, a porosity of 50%-80%, a tensile strength of 30-50 MPa, and an elongation at break of 10%-30%. The superhydrophobic polypropylene porous film maintains the chemical resistance, rigidity, and porosity of the polypropylene porous film, and has superhydrophobic properties and a good separation effect after working for 80 hours, thus greatly increasing the service life, and reducing operation costs and working costs in a membrane distillation process.

IPC Classes  ?

• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 71/26 - Polyalkenes
• B29C 41/00 - Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped articleApparatus therefor
• B29C 41/02 - Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped articleApparatus therefor for making articles of definite length, i.e. discrete articles
• B29C 41/52 - Measuring, controlling or regulating
• C23C 16/40 - Oxides
• C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
• C23C 16/56 - After-treatment
• B29K 23/00 - Use of polyalkenes as moulding material
• B29L 31/00 - Other particular articles

Abstract

Disclosed in the present invention is a metagenomics-based method for sampling a microorganism on a facial skin surface, comprising the following steps: (1) dipping a disposable medical disinfection cotton swab into sterile normal saline, and sampling an area specified by a disposable sterilization specification plate; (2) in a sterile room, collecting samples according to the sequence of left cheek, right cheek and forehead of a subject, and circling the cotton swab obtained in step (1) for 50 times in each area; and (3) putting the sampled cotton swab obtained in step (2) into a sterile Pbw solution, and storing at 4ºC for later use.

IPC Classes  ?

• C12Q 1/24 - Methods of sampling, or inoculating or spreading a sampleMethods of physically isolating an intact microorganism

Abstract

A large-particle spherical salt with a particle size of 400-950 μm and a sphericity of 0.5-1.0 is disclosed, which overcomes the existing difficulty in this field for larger particle size as well as higher sphericity. A preparation method of the large-particle spherical salt is also disclosed, wherein in one preparation process, 2% of gum arabic (based on the mass percentage of solute sodium chloride in a sodium chloride saturated solution) is added, and under conditions of an evaporating temperature of 60° C. a stirring rate of 350 rpm, and an evaporating time of 8 hours, a large-particle spherical salt with a particle size of 921.593 μm and an average sphericity of 0.904 is successfully prepared. The large-particle spherical salt prepared by the method has a uniform particle size distribution and good appearance, can be combined with other substances, adding some extra value to the salt. Meanwhile, the large-particle spherical salt prepared by the method has a high safety grade (e.g.: food grade) and can be used as edible salt, nutrient salt or foot bath salt.

IPC Classes  ?

• C01D 3/24 - Influencing the crystallisation process

Abstract

The present application provides use of Monascinol in preparation of a fat-reducing product, a method for preventing or treating a disease related to abnormal lipid metabolism, and a fat-reducing product including Monascinol. The fat-reducing product includes a fat-reducing functional food and a fat-reducing drug, the fat-reducing functional food is used for preventing or improving a sub-health state related to obesity in an individual, the fat-reducing drug is used for preventing or treating a disease related to abnormal lipid metabolism in an individual. It is found that in the solution of the present application the Monascinol has new functions of significantly lowering blood lipid, controlling weight gain, and inhibiting body fat accumulation; and has low cytotoxicity, thereby having high use safety when serving as a fat-reducing product.

IPC Classes  ?

• A61K 31/352 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. cannabinols, methantheline
• A61P 1/16 - Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• A23L 33/00 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof
• A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives

Abstract

A large-particle spherical salt. The particle size of the large-particle spherical salt is 400-950 µm, and the sphericity of the large-particle spherical salt is 0.5-1.0. Therefore, the present invention solves the two problems of requiring a large particle size and requiring high sphericity. Further disclosed is a preparation method for the large-particle spherical salt. The method comprises: during preparation, pertinently adding 2% of Arabic gum (based on the percentage by mass of a solute, i.e., sodium chloride, in a saturated sodium chloride solution), and under the conditions that an evaporation temperature is 60°С, a stirring rate is 350 r/min, and an evaporation period of time is 8 h, successfully preparing the large-particle spherical salt having the particle size of 921.593 µm and the sphericity of 0.904. The large-particle spherical salt prepared by means of the method is uniform in particle size distribution and good in appearance, and may be bound to other substances so as to increase the additional value of the salt. Moreover, the safety level of the prepared large-particle spherical salt reaches food grade, and the large-particle spherical salt may be used for edible salt, nutrient salt, or foot bath salt.

IPC Classes  ?

• C01D 3/22 - Preparation in the form of granules, pieces, or other shaped products
• C01D 3/24 - Influencing the crystallisation process

Abstract

A lactase for producing galactooligosaccharides, preparation therefor and use thereof are provided. The lactase is a novel lactase enzyme molecule which has high efficiency for oligomerizing galactose and good expression performance and is obtained by evolution based on lactase molecules from two sources. Also provided is a lactase high-yielding strain which can efficiently synthesize a lactase during submerged fermentation and secrete enzyme protein molecules into a culture medium, so as to directly prepare a highly active enzyme formulation from a fermentation supernatant.

IPC Classes  ?

• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)
• C12N 15/75 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 19/00 - Preparation of compounds containing saccharide radicals
• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12R 1/10 - Bacillus licheniformis
• C12R 1/07 - Bacillus

Abstract

A superhydrophobic polypropylene porous film, comprising a polypropylene porous film substrate, a titanium dioxide layer, and a surface modifier layer. The titanium dioxide layer is deposited on the surface of the polypropylene porous film substrate by means of the atomic deposition technology; a surface modifier is coated on the titanium dioxide layer; hydrophobic bonds are formed between the titanium dioxide layer and the surface modifier layer; the water contact angle of the superhydrophobic polypropylene porous film is greater than 150 degrees, the rolling angle is less than 10 degrees, the aperture is 0.1-0.4 μm, the porosity is 50%-80%, the tensile strength is 30-50 MPa, and the elongation at break is 10%-30%. The superhydrophobic polypropylene porous film not only maintains the chemical resistance, rigidity, and porosity of the polypropylene porous film, but also has superhydrophobic properties, and still maintains a good separation effect after working for 80 hours, thus greatly increasing the service life, and reducing operation costs and working costs of a film distillation process.

IPC Classes  ?

• B01D 71/26 - Polyalkenes
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 61/36 - PervaporationMembrane distillationLiquid permeation
• B01D 61/14 - UltrafiltrationMicrofiltration

Abstract

A signal peptide mutant for improving the secretion of a heterologous protein, a construction method therefor and an application thereof, which belong to the technical field of genetic engineering. The signal peptide mutant in the present invention is obtained by: performing site-directed mutation on charged amino acids in an N region of a signal peptide derived from the Sec pathway of Gram-positive bacteria so that the charge density of the amino acids in the N region is between 0.2 and 0.8; mutating an H region of the signal peptide so that the amino acid hydrophobicity of the H region is between 0-70; and performing site-directed mutation on amino acids in a C region of the signal peptide so that the last three amino acids in the C region are mutated to alanine-any amino acid-alanine. The present invention effectively increases the secretion of a heterologous protein in a microbial host, and promotes the high-efficiency expression and industrial production of a target protein.

IPC Classes  ?

• C07K 14/32 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Bacillus (G)
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

A lignocellulose nanofibril material, a stable foam system based thereon, a preparation method and an application thereof are provided. The lignocellulosic nanofibril material includes the following components: 0.5-20 wt % of wood flour, 0.1-10 wt % of (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl, 2-25 mmol/g of an oxidant, 6-15 wt % of NaBr, and the remaining is water. The stable foam system based on the lignocellulosic nanofibril material includes: 0.1-1.0 wt % of the lignocellulosic nanofibril material, 0.2-1.0 wt % of a surfactant, 0.1-10 wt % of sodium chloride, 0.1-1.0 wt % of calcium chloride, 0.1-1.0 wt % of magnesium chloride, 0.1-1.0 wt % of sodium sulfate, and a balance of water.

IPC Classes  ?

• C09K 8/94 - Foams
• C08H 8/00 - Macromolecular compounds derived from lignocellulosic materials
• C08J 9/00 - Working-up of macromolecular substances to porous or cellular articles or materialsAfter-treatment thereof
• C08J 9/30 - Working-up of macromolecular substances to porous or cellular articles or materialsAfter-treatment thereof by mixing gases into liquid compositions or plastisols, e.g. frothing with air
• C09K 8/58 - Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids

Abstract

Saccharomyces cerevisiae strain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.

IPC Classes  ?

• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12G 3/021 - Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye or corn
• C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12P 7/62 - Carboxylic acid esters

Abstract

An application of monascinol in the preparation of a fat-reducing product. The fat-reducing product comprises a fat-reducing functional food or a fat-reducing drug; the fat-reducing functional food is used for preventing or improving the sub-health status related to obesity of an individual; and the fat-reducing drug is used for preventing or treating diseases related to abnormal lipid metabolism of an individual. Monascinol has new functions of significantly reducing blood lipids, controlling weight gain and inhibiting body fat accumulation; in addition, same has reduced cytotoxicity and has higher usage safety as a fat-reducing product.

IPC Classes  ?

• A61K 31/352 - Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. cannabinols, methantheline
• A61P 3/04 - AnorexiantsAntiobesity agents
• A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives

Abstract

Provided is an air purifier, comprising a control unit (1), a purifier body (2), a filtering device (3), a first blower (4), an air ventilating pipe (5) and a chemical reaction unit (6). The purifier body (2) is provided with an air intake channel (21), an air inlet (22), an accommodating cavity (23), an air exhaust channel (24), and an air outlet (25). The air inlet (22) is arranged at the first end of the air intake channel (21), one end of the air exhaust channel (24) is in communication with the accommodating cavity (23), and the other end thereof is in communication with the air outlet (25). The first blower (4) is detachably arranged in the air intake channel (21); the filtering device (3) is arranged between the first blower (4) and the air inlet (22); the chemical reaction unit (6) comprises a reaction container (61) and a reactant; and the air ventilating pipe (5) is arranged in the purifier body (2), a first end thereof is connected to the air exhaust end of the first blower (4), and a second end thereof extends into the reactant.

IPC Classes  ?

• F24F 3/16 - Air-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatmentApparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling by purification, e.g. by filteringAir-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatmentApparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling by sterilisationAir-conditioning systems in which conditioned primary air is supplied from one or more central stations to distributing units in the rooms or spaces where it may receive secondary treatmentApparatus specially designed for such systems characterised by the treatment of the air otherwise than by heating and cooling by ozonisation
• F24F 1/02 - Self-contained room units for air-conditioning, i.e. with all apparatus for treatment installed in a common casing
• F24F 13/28 - Arrangement or mounting of filters

Abstract

M for relieving feedback inhibition by L-isoleucine, a 3-isopropyl malate dehydrogenase coding gene leuB and a 3-isopropyl malate dehydratase coding gene leuCD in host cells. The genetically engineered bacterium for producing the L-leucine is free from nutritional deficiency, rapid in growth, short in fermentation period, high in yield and high in conversion rate.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine

Abstract

Disclosed are gene engineering bacteria for producing L-arginine and a construction method and an application of the gene engineering bacteria. According to the method, a gene for encoding carbamyl phosphate synthetase and a gene for encoding an L-arginine biosynthetic pathway enzyme are integrated in Escherichia coli; a synthetic pathway of arginine in Escherichia coli and metabolic flux related to arginine in a whole amino acid metabolic network are analyzed and reconstructed to obtain gene engineering bacteria which are clear in genetic background, do not carry plasmids, are not mutated and can stably and efficiently produce L-arginine.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 13/10 - CitrullineArginineOrnithine
• C12R 1/19 - Escherichia coli

Abstract

Provided are a 2-isopropylmalate synthase, L-leucine-producing genetically engineered bacteria, and applications thereof, related to the field of metabolic engineering. The genetically engineered bacteria are acquired by overexpressing in a host cell L-leucine feedback inhibition-releasing isopropylmalate synthase coding gene leuAM, L-isoleucine feedback inhibition-releasing acetolactate synthase coding gene ilvBNM, 3-isopropylmalate dehydrogenase coding gene leuB, and 3-isopropylmalate dehydratase coding gene leuCD. The L-leucine genetically engineered bacteria are free of auxotrophy, grow rapidly, and have a short fermentation cycle, high yield, and high conversion rate.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine

Abstract

A use for an Ulva polysaccharide in suppressing β-amyloid protein aggregation, relating to the technical fields of medicine, health products, or food products. When the Ulva polysaccharide is used in preparing a drug, a health product, or a food product, the Ulva polysaccharide is able to effectively suppress β-amyloid protein aggregation, thereby preventing the occurrence of Alzheimer's disease. Within a certain range of concentrations, the suppression result improves along with increased Ulva polysaccharide concentration. The Ulva polysaccharide changes the morphology of aggresomes, preventing and slowing down the transformation thereof into a fibrous morphology. Meanwhile, cytotoxicity caused by aggresomes formed in a process of β-amyloid protein aggregation is effectively suppressed.

IPC Classes  ?

• A61K 31/715 - Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkagesDerivatives thereof, e.g. ethers, esters
• A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• A61K 36/05 - Chlorophycota or chlorophyta (green algae), e.g. Chlorella
• A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates

Abstract

Aureobasidium pullulans recombinant strain can significantly increase the yield of heavy oil. After 7-day fermentation with xylose as carbon source, the yield of the heavy oil of the recombinant strain reaches 19.4372 g/L, while the yield of the heavy oil of the original strain is 10.0325 g/L, i.e. the recombinant strain improves the yield by 93.74% compared with the original strain.

IPC Classes  ?

• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12N 9/88 - Lyases (4.)
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12P 7/6463 - Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Abstract

The present invention discloses a novel nano-composite antibacterial material and a preparation method and an application thereof, and belongs to the technical field of preservative materials. The novel nano-composite antibacterial material disclosed by the present invention is prepared by mixing a dimethylimidazole solution, deionized water and a zinc nitrate solution to prepare a metal-organic framework carrier, compositing with nisin to form a nano antibacterial composite material, separating out from a solution in a precipitate form, centrifuging, removing a supernatant, cleaning and re-suspending with the deionized water. The novel nano-composite antibacterial material prepared by the present invention has an antibacterial effect superior to nisin having a same concentration. The present invention prominently improves an antibacterial activity and thermostability of the nisin under neutral and slightly alkaline conditions, effectively promotes antibacterial property and a usable range of the nisin, and further expands an application field of the metal-organic framework carrier.

IPC Classes  ?

• C07K 17/02 - Peptides being immobilised on, or in, an organic carrier
• B82Y 30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites
• A23L 3/3463 - Organic compounds; Microorganisms; Enzymes
• A23B 4/20 - Organic compoundsMicroorganismsEnzymes
• A23B 7/154 - Organic compoundsMicroorganismsEnzymes
• B82Y 5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• B82Y 40/00 - Manufacture or treatment of nanostructures

Abstract

Disclosed is a non-sintering method for preparing artificial cobblestones from dredging soil, the method comprising the steps of: (1) preparing raw materials; (2) proportioning four types of materials; (3) preparing high-strength non-sintering ceramsite; (4) preparing cobblestone cores; (5) preparing an initial product, cobblestones; (6) polishing; (7) curing; and (8) forming a finished product. Preparing artificial cobblestones of a core-shell structure by using dredging soil as the raw material can not only expand the application range of the resource utilization of dredging soil, but also provide, for artificial cobblestones, a preparation method, wherein the preparation involves uses a non-sintering method, the energy consumption for production is low, and the decorative effect of cobblestones can be achieved.

IPC Classes  ?

• C04B 28/02 - Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements containing hydraulic cements other than calcium sulfates
• C04B 111/54 - Substitutes for natural stone, e.g. artificial marble

Abstract

A method for preparing a water permeable brick using dredged sediment firing-free aggregate. The method comprises the following steps of: (1) screening the dredged sediment firing-free aggregate; (2) preparing pretreated firing-free aggregate; (3) preparing base layer ingredients; (4) adding water to the base layer ingredients and stirring the mixture; (5) preparing face layer ingredients; (6) adding water to the face layer ingredients and stirring the mixture; (7) performing press molding by a brick machine; and (8) maintaining the manufactured product. The method can consume a large amount of dredged sediment, reduces exploitation of natural aggregate, achieves the purposes of recycling and ecological environment protection, and meets demands of dredging industry; the used firing-free method is low in production energy consumption and less in emission, the produced water permeable brick is low in cost and excellent in performance, the dredged sediment recycling approaches can be widened, the demand of a building material market can be met, and the "urban heat island effect" can be well relieved.

IPC Classes  ?

• B28B 3/00 - Producing shaped articles from the material by using pressesPresses specially adapted therefor
• C04B 28/04 - Portland cements

Abstract

Provided is a compound represented by formula (I) serving as an inhibitor of cyclin-dependent kinase 7 (CDK7), a pharmaceutical composition thereof, and a method of using the same. Proposed is a use of the compound or the pharmaceutical composition. Representative compounds show anti-cell proliferation and anti-angiogenic activity. Some compounds are more effective than existing anticancer drugs on the market, such as sunitinib. Accordingly, in some aspects, the compound can be used for preparing drugs for treating abnormal cell proliferation or diseases related to angiogenesis, such as antitumor drugs, leukemia drugs and antiviral drugs.

IPC Classes  ?

• C07D 473/16 - Heterocyclic compounds containing purine ring systems with oxygen, sulfur, or nitrogen atoms directly attached in positions 2 and 6 two nitrogen atoms
• A61K 31/52 - Purines, e.g. adenine
• A61P 35/00 - Antineoplastic agents
• A61P 35/02 - Antineoplastic agents specific for leukemia
• A61P 31/12 - Antivirals

Abstract

A tubular rotary falling-film evaporator. A feed liquid distributor (8) is connected at the top of an evaporator shell (5), and a feed liquid intake (9) of the feed liquid distributor (8) extends from the evaporator shell (5). A falling-film evaporation tube fixing frame (12) is arranged horizontally inside the evaporator shell (5), and falling-film evaporation tubes (6) are distributed in parallel on the falling-film evaporation tube fixing frame (12). A plurality of feed liquid nozzles (11) of the feed liquid distributor (8) extend into respective falling-film evaporation tubes (6). A transmission shaft (14) is connected to the falling-film evaporation tube frame (12). The top end of the transmission shaft (14) is connected to the feed liquid distributor (8), and the bottom end of the transmission shaft (14) extends out of the evaporator shell (5) and is connected to an output shaft of a variable frequency motor (16) by means of meshing of a gear (1) and a gear ring (15).

IPC Classes  ?

• B01D 1/22 - Evaporating by bringing a thin layer of the liquid into contact with a heated surface
• B01D 1/30 - Accessories for evaporators

Abstract

Disclosed are a citric acid-producing microorganism strain and a method for producing citric acid by fermenting starch sugar therefor. The strain is Aspergillus niger 101-HAC11 with the he preservation number of CGMCC No.12480. Moreover, also disclosed are a culture medium formula and a fermentation process control process for producing citric acid by using starch sugar as a carbon source. The yield of citric acid produced by the strain is 166.8 g/L. Compared with the prior art, the citric acid yield of the recombinant Aspergillus niger strain is 220.34 g/L, and the yield is increased by 32.10%. The method has high speed, simple production process, high saccharic acid conversion rate, and high production efficiency, and is applicable to automated large-scale industrial production, and the products are easy to separate.

IPC Classes  ?

• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/53 - Oxidoreductases (1)
• C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
• C12P 7/48 - Tricarboxylic acids, e.g. citric acid

Abstract

Disclosed are a citric acid-producing microorganism strain and a method for producing citric acid by fermenting starch sugar therefor. The strain is Aspergillus niger 101-HAC11 with the he preservation number of CGMCC No.12480. Moreover, also disclosed are a culture medium formula and a fermentation process control process for producing citric acid by using starch sugar as a carbon source. The yield of citric acid produced by the strain is 166.8 g/L. Compared with the prior art, the citric acid yield of the recombinant Aspergillus niger strain is 220.34 g/L, and the yield is increased by 32.10%. The method has high speed, simple production process, high saccharic acid conversion rate, and high production efficiency, and is applicable to automated large-scale industrial production, and the products are easy to separate.

IPC Classes  ?

• C12R 1/685 - Aspergillus niger
• C12P 7/48 - Tricarboxylic acids, e.g. citric acid

Abstract

A modular phase-change energy storage heat exchanger. Phase-change materials are filled around circulating-water conveying pipes (13) and antifreeze fluid conveying pipes (12) in a case (5). One side of an opening portion of the case (5) is provided with a notch. A connector box (6) is embedded in the notch. The connector box (6) is evenly divided into four cavities by means of a cross-shaped partition (6-5). An outer side wall of an antifreeze fluid inlet cavity (6-3) is in communication connection with an antifreeze fluid inlet pipe connector. An outer side wall of an antifreeze fluid outlet cavity (6-8) is in communication connection with an antifreeze fluid outlet pipe connector. Two ends of the two antifreeze fluid conveying pipes (12) are respectively connected to the antifreeze fluid inlet cavity (6-3) and the antifreeze fluid outlet cavity (6-8). An outer side wall of a circulating-water inlet cavity (6-6) is in communication connection with a circulating-water inlet pipe connector. An outer side wall of a circulating-water outlet cavity (6-1) is in communication connection with a circulating-water outlet pipe connector. Two ends of the two circulating-water conveying pipes (13) are respectively connected to the circulating-water inlet cavity (6-6) and the circulating-water outlet cavity (6-1). By means of the modular "battery-type" design of the heat exchanger, cases can be connected in series according to the actual requirements of a user, such that the use thereof is not limited by an intrinsic energy storage capacity of the cases.

IPC Classes  ?

• F28D 20/02 - Heat storage plants or apparatus in generalRegenerative heat-exchange apparatus not covered by groups or using latent heat
• F28F 9/02 - Header boxesEnd plates

Abstract

Dried fresh jujube chips and an energy-saving processing technique for differential-pressure explosion puffing drying. The technical method is especially applicable to production of dried fresh jujube chips, solves the industrial problems of advanced oxidative browning of the dried fresh jujube chips during hot-air drying at a high temperature of 90-110°C and of high water loss speed due to drying for 6-8 hours making the jujube chips have tight cell textures themselves when dried and becoming firmer and hard to chew, and also solves the production problems of high energy consumption in a vacuum of 1.3-13Pa and -10 - -50°C cryogenic drying, long processing cycle time (3-5 hours), major equipment investment, and the chips having small textures and being too mushy to have a satisfying mouthfeel when chewing, and enables the colour, aroma and taste and texture of the dried fresh jujube chips subjected to differential-pressure explosion puffing drying to have the best quality. The production steps comprise: (1) fresh jujube slicing preparation, (2) stone removal and stem removal, (3) dried fresh jujube quick-freezing and preservation, (4) thawing of frozen dried fresh jujubes, (5) colour protection treatment, (6) differential-pressure explosion puffing of dried jujubes, (7) mixed superfine seasoning powder preparation and (8) superfine seasoning powder coating.

IPC Classes  ?

• A23L 19/00 - Products from fruits or vegetablesPreparation or treatment thereof

Abstract

A method for processing dried fresh jujube slices includes the following steps: preparing jujube slices, coring and removing stems, quick-freezing and retaining freshness of the jujube slices, thawing frozen jujube slices, protecting color treatment, expanding jujube slices under differential pressure, preparing superfine mixed seasoning powder, and coating of the superfine mixed seasoning powder. For one hand, this method solves problems that the dried fresh jujube slices will be deeply oxidized and brown when dried by the hot air at a high temperature of 90-110° C., the jujube slices with compacted cells will become harder and be difficult to chew because of 6-8 h rapid dehydration. On the other hand, it solves problems that exist in freeze drying at a vacuum degree of 1.3-13 Pa, and at a low temperature of −10-50° C., such as high energy consumption, long processing cycle, high equipment investment, small size of slices, and poor taste.

IPC Classes  ?

• A23L 19/00 - Products from fruits or vegetablesPreparation or treatment thereof
• A23B 7/045 - Thawing subsequent to freezing
• A23B 7/06 - Blanching
• A23L 5/41 - Retaining or modifying natural colour by use of additives, e.g. optical brighteners
• A23L 3/015 - Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress

Abstract

A system and method for use in freezing and coating after impact of micron-sized droplets onto spherical surfaces. The system comprises a droplet impact test platform and an image acquisition visualization system. The droplet impact test platform consists of a low temperature control system (1), an electrostatic atomizer (6), a particle distribution plate (11), and a lifting platform (13). The electrostatic atomizer (6) is placed on the upper end of the low temperature control system (1). Spherical particles (10) are arranged on the particle distribution plate (11). The particle distribution plate (11) is placed on a ribbed plate of the lifting platform (13), and located inside the low temperature control system (1) at a position directly below a nozzle (8). The image acquisition visualization system consists of a high speed camera (2), an LED light source (12), and a PC (4). The PC (4) is connected to a port of the high speed camera (2). The high speed camera (2) and the LED light source (12) are symmetrically placed at both ends of the lifting platform (13) with the lens and the light source being at the same height as the centers of the spherical particles (10). The test operating condition is a low temperature condition. Images about freezing and coating after droplets impact onto the spherical particles (10) can be acquired clearly. The invention is suitable for study of coating and freezing after impact of droplets onto solid surfaces.

IPC Classes  ?

• G01N 15/00 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials

Abstract

A fruit and vegetable phase temperature pre-cooling-compatible multi-coupling anti-aging treatment equipment, the equipment comprising a cold storage (11), a plurality of differential pressure pre-cooling devices being placed within the cold storage (11); the differential pressure pre-cooling devices comprise an air-tight tunnel pre-cooling tank (3), and the air-tight tunnel pre-cooling tank (3) is provided therein with an ozone generating device (14) and an ultraviolet sterilization device (15). The equipment employs a manner of full enclosure, which may reduce moisture loss for products during pre-cooling, reduce cooling capacity loss during pre-cooling, and accelerate pre-cooling effeciency.

IPC Classes  ?

• A23B 7/04 - FreezingSubsequent thawingCooling

Abstract

Disclosed is a method for comprehensive recycling of a by-product slurry during polyphenylene sulfide (PPS) production. Na2CO3 is added to the slurry, and then a solvent NMP is recovered by distillation and drying, then an acid solution is used for dissolving and leaching the resulting dry waste salt, the leaching liquor is subjected to pH adjustment and then filtered, with the insoluble components being removed, the liquid phase is subjected to adsorption and purification and evaporation concentration to separate out NaCl, then Na2CO3 is added to the solution with NaCl separated out for precipitation to obtain Li2CO3, the mother liquor with lithium precipitated is subjected to pH adjustment and then mixed with the solution obtained after adsorption and purification, and a closed circulation of the process is achieved. The method can be used in the comprehensive recycling of the by-product slurry during PPS production, the recovered NaCl can be directly used for caustic soda preparation by ionic membrane electrolysis, and Li2CO3 can directly serve as an industrial product or can be further converted into LiCl to serve as a PPS production auxiliary for recycling.

IPC Classes  ?

• C08G 75/0277 - Post-polymerisation treatment
• C08G 75/0281 - Recovery or purification
• C08G 75/0254 - Preparatory processes using metal sulfides
• C08G 75/0213 - Polyarylenethioethers derived from monomers containing one aromatic ring containing elements other than carbon, hydrogen or sulfur
• C01D 15/08 - CarbonatesBicarbonates
• C01D 3/14 - Purification
• C07D 207/267 - 2-Pyrrolidones with only hydrogen atoms or radicals containing only hydrogen and carbon atoms directly attached to other ring carbon atoms with only hydrogen atoms or radicals containing only hydrogen and carbon atoms directly attached to the ring nitrogen atom

Abstract

Disclosed are very high optically pure D- and L-lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L-lactic acids using the strains, wherein the deposit number of the D-lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L-lactic acid fermentation production strain is CGMCC No. 11060.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 7/56 - Lactic acid
• C12R 1/19 - Escherichia coli

Abstract

Disclosed are very high optically pure D- and L- lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L- lactic acids using the strains, wherein the deposit number of the D- lactic acid fermentation production strain is CGMCC No.11059, and the deposit number of the L- lactic acid fermentation production strain is CGMCC No.11060.

IPC Classes  ?

• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12P 7/56 - Lactic acid
• C12R 1/19 - Escherichia coli

Abstract

Provided is a system for preparing large-particle potassium chloride by cold-decomposition crystallization and a flotation method with high-sodium potassic salt ore. The system is formed by sequentially connecting a feeding control system, a cold-decomposition crystallizer, a fine crystal removing tank, and a fresh water storage tank, a stirring paddle being provided in the cold-decomposition crystallizer, a circulating pump for delivering circulation fluid being mounted between the fine crystal removing tank and the cold-decomposition crystallizer, and a water pump being mounted on a supply pipe of the fresh water storage tank. Also provided is a process for preparing large-particle potassium chloride by cold-decomposition crystallization and a flotation method with high-sodium potassic salt ore.

IPC Classes  ?

• C01D 3/08 - Preparation by working up natural or industrial salt mixtures or siliceous minerals
• C01F 11/46 - Sulfates

Abstract

A high whiteness coated paper made from medium whiteness base paper and the coating process and the coating material thereof are provided. The high whiteness coated paper has a whiteness of 86-120% and is produced by coating a base paper, which has a whiteness of 55-83%, with a base coating and a top coating, wherein the base coating comprises (parts by weight): 10-90 parts of heavy calcium carbonate, 10-80 parts of kaolin, 5-20 parts of starch, 0-12 parts of latex adhesive, and 0-10 parts of titanium dioxide; and the top coating comprises (parts by weight): 0.3-2.0 parts of fluorescent whitening agent, 10-90 parts of heavy calcium carbonate, 10-80 parts of kaolin, 8-20 parts of latex adhesive, and 0-1.5 parts of polyvinyl alcohol or sodium carboxymethyl cellulose.

IPC Classes  ?

• D21H 19/82 - Paper comprising more than one coating superposed
• D21H 19/84 - Paper comprising more than one coating on both sides of the substrate
• D21H 19/54 - Starch
• D21H 21/18 - Reinforcing agents
• D21H 21/30 - Luminescent or fluorescent substances, e.g. for optical bleaching
• D21H 19/64 - Inorganic compounds
• D21H 19/60 - PolyalkenylalcoholsPolyalkenylethersPolyalkenylesters
• D21H 19/52 - CelluloseDerivatives thereof

Abstract

A method for enhancing the whitening efficiency of fluorescent whitening agent in two-layer or multilayer coated paper or paperboard by means of starch is provided. The content of starch in a base coating is 3-20% by total weight of the base coating. The content of fluorescent whitening agent in a top coating is 0.1-2.0% by total weight of the top coating. Because the method utilizes the characteristics that the starch can retain the fluorescent whitening agent on the surface of the coated paper and can partly or completely replace synthetic latex adhesive, the method is in favor of environmental protection, and greatly reduces the production cost of coated paper or paperboard.

IPC Classes  ?

• D21H 21/30 - Luminescent or fluorescent substances, e.g. for optical bleaching
• D21H 19/54 - Starch
• D21H 19/82 - Paper comprising more than one coating superposed
• D21H 19/84 - Paper comprising more than one coating on both sides of the substrate

Abstract

A producing method of coated box cardboard or kraft paperboard with low cost and the coated box cardboard or coated kraft paperboard produced using the method are disclosed. The method comprises steps of bottom coating with bottom coating material and surface coating with surface coating material on the recycled or unbleached box cardboard or the kraft paperboard. A double-layer or three-layer coating process is used. By means of the synergistic effect of the components in the coating layers and the different coating layers, the whitening efficiency of the optical brightening agent (OBA) in low-whiteness unbleached box cardboard is improved. The coating material is mainly composed of kaolin, carbonate, starch and latex adhesive etc. The amount of OBA is not higher than 1% of the total coating amount. The amount of titanium pigment is not higher than 5% of the total coating amount. The total coating amount of the coated box cardboard is not higher than 40g/m2, and the whiteness of the paper product is not lower than 76%.

IPC Classes  ?

• D21H 21/30 - Luminescent or fluorescent substances, e.g. for optical bleaching
• D21H 19/80 - Paper comprising more than one coating

Abstract

A method for producing Nostoc flagelliforme cells and extracellular polysaccharide thereof with high efficiency by following two phases: (1)inoculating N. flagelliforme cell seed solution into a culture container containing modified BG11 culture medium, and culturing for 8-10 days; and (2) when concentration of N. flagelliforme cells is 0.5-0.7 g/L, adding glucose to a final concentration of 1-20 g/L to the culture medium, culturing for 2-8 days, centrifuging culture solution, collecting N. flagelliforme cells, and taking liquid supernatant to extract extracellular polysaccharide of culture solution. The dry weight of N. flagelliforme cells obtained by the method is improved by 5-8 times, and the output of extracellular polysaccharide is improved by 15-31 times, compared with the single photoautotrophic cultivation method. The dry weight of cells is 3.0-5.0 g/L and the output of polysaccharide is 1.0-2.3 g/L. The method shortens culture cycle of N. flagelliforme cells, improves output of extracellular polysaccharide and utilization rate of glucose compared with single mixing culture method, and reduces bacterial infection rate and manufacturing cost.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12P 19/04 - Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• C12R 1/89 - Algae