Disclosed is a fluorescent response cartridge module. The fluorescent response cartridge module comprises: a cartridge having a receiving chamber formed therein; a sample received in the receiving chamber and injected with a fluorescent material; and an optical member disposed above the receiving chamber, wherein the optical member can guide excitation light introduced from a light source so that the excitation light passes through the receiving chamber and then strikes the optical member at an angle of incidence at which total reflection occurs. Various other embodiments may be possible.
G01N 21/41 - RefractivityPhase-affecting properties, e.g. optical path length
2.
Device for analyzing large-area sample based on image, device for analyzing sample based on image by using difference in medium characteristic, and method for measuring and analyzing sample using the same
Provided are a device for analyzing a large-area sample based on an image, a device for analyzing a sample based on an image by using a difference in medium characteristic, and a method for measuring and analyzing a sample by using the same. The device for analyzing a large-area sample includes a first sensor array including sensors disposed while being spaced apart from each other in a first direction, a second sensor array including sensors disposed while being spaced apart from each other in the first direction, and spaced apart from the first sensor array in a second direction, and a control unit that obtains image data for a cell included in the sample by using sensing data of the sensor on the sample, in which the sample is interposed between the first sensor array and the second sensor array.
G06V 10/143 - Sensing or illuminating at different wavelengths
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
The present disclosure relates to a fluorescence filter for measuring fluorescence generated by a measurement object and an image sensor module including the same, and includes an absorption filter transmitting light within a specific wavelength band generated by the measurement object including a fluorescent dye and absorbs light in the remaining wavelength bands, and a reflection filter that is disposed adjacent to the absorption filter, transmits light within a specific wavelength band generated by the measurement object, and reflects light in the remaining wavelength bands, wherein the absorption filter has a plurality of wells having a predetermined depth in which the measurement object is accommodated, and wherein the plurality of wells are disposed at regular intervals on an incident surface of the absorption filter to which external light is incident.
The present invention relates to a fluorescence filter for measuring fluorescence generated from a measurement target, and an image sensor module comprising same, the image sensor module comprising: an absorption filter configured to transmit light which is in a specific wavelength band and is generated from a measurement target including a fluorescent dye and absorb light in the remaining wavelength band; and a reflection filter disposed adjacent to the absorption filter and configured to transmit light which is in a specific wavelength band and is generated from the measurement target and reflect light in the remaining wavelength band, wherein the absorption filter has wells having a predetermined depth and allowing the measurement target including the fluorescent dye to be stably placed therein, and the plurality of wells are arranged at regular intervals on an incident surface of the absorption filter to which external light is incident.
Government of the United States of America, as Represented by the Secretary of Commerce (USA)
Inventor
Lee, Jong Muk
Reyes-Hernandez, Darwin R.
Nablo, Brian J.
Abstract
The present disclosure relates to a real-time quantification method of cell viability through a supravital dye uptake using a lens-free imaging system. The method includes a step of incubating a sample cell in a cell culture medium, steps of detecting light penetrating the cell culture medium and identifying a boundary region of the sample cell at a preset time interval based on the detected light, a step of staining the incubated sample cell with the supravital dye, a step of detecting intensity of light penetrating the cell culture medium at a preset time interval, a step of calculating absorbance of the sample cell included in the cell culture medium at a preset time interval based on the boundary region and the detected intensity of light and a step of analyzing a viability of the sample cell based on the calculated absorbance.
An image sensor package, a system, and a method for counting fine particles by using a virtual grid line are provided. The image sensor package includes an image sensor array, a grid pattern layer formed on an outer area of the image sensor array and including a plurality of protruding patterns spaced apart from each other while protruding toward the central area of the image sensor array to form a virtual grid line, a dam pattern layer formed on the grid pattern layer, having a specific height, and configured to form a channel or a chamber for receiving the fine particles to be counted, and a cover glass formed on the dam pattern layer.
G06T 11/20 - Drawing from basic elements, e.g. lines or circles
G01N 15/01 - Investigating characteristics of particlesInvestigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
7.
Method for correcting optical sensor array module through characteristic evaluation
The present invention relates to a method for correcting a packaged optical sensor array module, and the method for correcting a packaged optical sensor array module according to the present invention comprises the steps of: analyzing statistical characteristics of an optical sensor array with respect to light emitted from a standard light source having a predetermined characteristic value to extract a representative value, and calculating a first correction value for a measurement value according to the extracted representative value; and calculating a second correction value for a measured value of the optical sensor array that is corrected by the first correction value with respect to light emitted from an applied light source or light emitted by a fluorescence of the applied light source.
H04N 5/357 - Noise processing, e.g. detecting, correcting, reducing or removing noise
H04N 5/374 - Addressed sensors, e.g. MOS or CMOS sensors
H04N 25/60 - Noise processing, e.g. detecting, correcting, reducing or removing noise
H04N 25/76 - Addressed sensors, e.g. MOS or CMOS sensors
H04N 25/671 - Noise processing, e.g. detecting, correcting, reducing or removing noise applied to fixed-pattern noise, e.g. non-uniformity of response for non-uniformity detection or correction
H04N 25/672 - Noise processing, e.g. detecting, correcting, reducing or removing noise applied to fixed-pattern noise, e.g. non-uniformity of response for non-uniformity detection or correction between adjacent sensors or output registers for reading a single image
H04N 25/673 - Noise processing, e.g. detecting, correcting, reducing or removing noise applied to fixed-pattern noise, e.g. non-uniformity of response for non-uniformity detection or correction by using reference sources
8.
Device for analyzing large-area sample based on image, device for analyzing sample based on image by using difference in medium characteristic, and method for measuring and analyzing sample using the same
Provided are a device for analyzing a large-area sample based on an image, a device for analyzing a sample based on an image by using a difference in medium characteristic, and a method for measuring and analyzing a sample by using the same. The device for analyzing a large-area sample includes a first sensor array including a plurality of sensors which are disposed while being spaced apart from each other in a first direction, a second sensor array including a plurality of sensors, which are disposed while being spaced apart from each other in the first direction, and spaced apart from the first sensor array in a second direction, and a control unit to obtain image data for a cell included in the sample by using sensing data of the sensor on the sample, in which the sample is interposed between the first sensor array and the second sensor array. An active area of one of the sensor in the first sensor array overlaps an active area of one of the sensors in the second sensor array, in the second direction.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 21/17 - Systems in which incident light is modified in accordance with the properties of the material investigated
9.
IMAGE SENSOR PACKAGE FOR COUNTING FINE PARTICLES BY USING VIRTUAL GRID LINE, AND SYSTEM AND METHOD FOR COUNTING FINE PARTICLES
Provided are an image sensor package for counting fine particles by using a virtual grid line, and a system and a method for counting fine particles. The image sensor package comprises: an image sensor array; a grid pattern layer for forming a virtual grid line, the grid pattern layer being formed on the outer edge area of the image sensor array and including a plurality of protruding patterns protruding toward the central area of the image sensor array while being spaced a predetermined distance apart from each other; a dam pattern layer for forming a channel, the dam pattern layer being formed on the grid pattern layer, having a predetermined height, and forming a channel or a chamber in which fine particles to be counted are received; and a cover glass formed on the dam pattern layer.
G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
10.
IMAGE SENSOR MODULE, SMALL DIGITAL MICROSCOPE AND SMALL DIGITAL MICROSCOPE ARRAY SYSTEM, REAL-TIME THREE-DIMENSIONAL DIGITAL MICROSCOPE, AND DIGITAL MICROSCOPE AND DIGITAL MICROSCOPE SYSTEM IN WHICH HIGH MAGNIFICATION IMAGE ISGUIDED BY LOW MAGNIFICATION IMAGE
The present invention relates to an image sensor module, a small digital microscope and a small digital microscope array system, a real-time three-dimensional digital microscope, and a digital microscope and a digital microscope system in which a high magnification image is guided by a low magnification image. The image sensor module is characterized by comprising: a mount to which an objective lens module for determining the magnification of a captured image is coupled; and a lens-free image sensor on which light transmitted through the objective lens module is incident, wherein the microlens shift value of each pixel of the lens-free image sensor is designed in correspondence to the chief ray angle (CRA) value of the objective lens module.
The present invention relates to an image sensor-based bio-diagnostic device utilizing lateral light, and an integrated cartridge. The image sensor-based bio-diagnostic device utilizing lateral light has a space in which a sample accommodation member is disposed, and comprises: an image sensor package positioned on one side of the space in which the sample accommodation member is disposed; a light guide element disposed between the space in which the sample accommodation member is disposed and the image sensor package; and a light source element for emitting light at the light guide element, wherein the light guide element guides at least a portion of the light emitted from the light source element toward the space in which the sample accommodation member is disposed.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G01N 33/483 - Physical analysis of biological material
12.
IMAGE-BASED LARGE-AREA SAMPLE ANALYSIS DEVICE, AND IMAGE-BASED SAMPLE ANALYSIS DEVICE USING MEDIUM CHARACTERISTIC DIFFERENCE AND METHOD FOR MEASURING AND ANALYZING SAMPLE BY USING SAME
The present invention relates to an image-based large-area sample analysis device, an image-based sample analysis device using a medium characteristic difference and a method for measuring and analyzing a sample by using same, the large-area sample analysis device comprising: a first sensor array including a plurality of sensors arranged at intervals in a first direction; a second sensor array which includes a plurality of sensors arranged at intervals in the first direction and which is spaced in a second direction from the first sensor array; and a control unit for acquiring image data for cells included in the sample, by using sensing data of the sensor for the sample arranged between the first sensor array and the second sensor array, wherein an effective area of any one of the sensors in the first sensor array overlaps in the second direction with an effective area of at least one of the sensors in the second sensor array.
G01N 21/31 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
G01N 21/17 - Systems in which incident light is modified in accordance with the properties of the material investigated
13.
Method for evaluating fluid flow characteristics of lens-free CMOS optical array sensor package module having flow channel
Provided is a method for evaluating fluid flow characteristics of a lens-free complementary metal-oxide semiconductor (CMOS) optical sensor package module with a flow channel. The method includes: measuring a propagation profile and a flow velocity in an initial state flow of a fluid in the flow channel; calculating a first statistical parameter relating to flow characteristics of the fluid from the measured propagation profile and flow velocity; and comparing the calculated first statistical parameter with a preset reference value and evaluating quality of the flow channel according to the comparison result.
The present invention relates to a method for correcting a packaged optical sensor array module, and the method for correcting a packaged optical sensor array module according to the present invention comprises the steps of: analyzing statistical characteristics of an optical sensor array with respect to light emitted from a standard light source having a predetermined characteristic value to extract a representative value, and calculating a first correction value for a measurement value according to the extracted representative value; and calculating a second correction value for a measured value of the optical sensor array that is corrected by the first correction value with respect to light emitted from an applied light source or light emitted by a fluorescence of the applied light source.
The present invention relates to a method for evaluating fluid flow characteristics of a lens-free CMOS optical sensor package module having a flow channel, the method comprising the steps of: measuring a propagation profile and a flow velocity in an initial flow state of a fluid in the flow channel; calculating a first statistical parameter relating to the flow characteristics of the fluid from the measured propagation profile and flow velocity; and comparing the calculated first statistical parameter with a predetermined reference value, and evaluating the quality of the flow channel according to the comparison result.
The present invention relates to a method for correcting a packaged optical sensor array module, and the method for correcting a packaged optical sensor array module according to the present invention comprises the steps of: analyzing statistical characteristics of an optical sensor array for light emitted from a standard light source having a predetermined characteristic value so as to extract a representative value, and calculating a first correction value for a measurement value according to the extracted representative value; and calculating a second correction value for the measured value of the optical sensor array, corrected by the first correction value, with respect to light emitted from an applied light source or light emitted by fluorescence to the applied light source.
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