Abstract

An object is to provide a plant genome editing technique with higher genome editing efficiency. This object is achieved by a polynucleotide comprising a site-specific nuclease expression cassette and a sequence that binds to a nuclear translocation factor and/or an intranuclear structure, the site-specific nuclease expression cassette comprising (b) a site-specific nuclease coding sequence and (c) a 3′UTR comprising a terminator.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

Provided is a genome editing technique that achieves higher genome editing efficiency. Any of Proteins 1 to 3 of the present invention.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Provided is a plant transformation technique that is more efficient and widely usable even for species in which seed gathering is difficult.　(c) This method for producing a plant introduced with a polynucleotide includes introducing the polynucleotide into a callus derived from a stem apical tissue of the plant to obtain a callus introduced with the polynucleotide.

IPC Classes  ?

• A01H 1/00 - Processes for modifying genotypes
• A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• C12N 15/87 - Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

To provide a genome-editing technique having higher genome-editing efficiency. Any of proteins 1-3 of the present invention.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• A01K 67/027 - New or modified breeds of vertebrates
• C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
• C07K 19/00 - Hybrid peptides
• C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 9/22 - Ribonucleases
• C12N 15/55 - Hydrolases (3)
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

The present invention addresses the problem of providing plant genome editing technology with higher genome editing efficiency. The problem addressed by the present invention is solved by a polynucleotide including a site-specific nuclease expression cassette and a sequence that binds to a nuclear translocation factor and/or an intranuclear structure, wherein the site-specific nuclease expression cassette includes (b) a site-specific nuclease coding sequence and (c) a 3'UTR including a terminator.

IPC Classes  ?

• C12N 15/55 - Hydrolases (3)
• A01H 1/00 - Processes for modifying genotypes
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/83 - Viral vectors, e.g. cauliflower mosaic virus

Abstract

An object is to provide a plant genome editing technique with higher genome editing efficiency. This object is achieved by a polynucleotide comprising a site-specific nuclease expression cassette and a sequence that binds to a nuclear translocation factor and/or an intranuclear structure, the site-specific nuclease expression cassette comprising (b) a site-specific nuclease coding sequence and (c) a 3?UTR comprising a terminator.

IPC Classes  ?

• A01H 1/00 - Processes for modifying genotypes
• C12N 15/09 - Recombinant DNA-technology
• C12N 15/55 - Hydrolases (3)
• C12N 15/83 - Viral vectors, e.g. cauliflower mosaic virus

Abstract

Provided is a grafting device that can improve productivity while suppressing variations in the quality of grafted seedlings. The present disclosure provides a grafting device for performing grafting using a grafting member including: a support member having an abutting portion with which the stem of a grafting plant abuts; and a fixed member having a pressing part for pressing the stem against the abutting portion. The grafting device comprises: a gripping part that grips the grafting plant and arranges the grafting plant on the support member; an attachment part that attaches the fixed member to the support member so that the stem of the grafting plant is pressed against the abutting portion; a cutting part that obtains a scion and a rootstock by cutting the grafting plant and divides the support member into a scion unit that holds the scion and a rootstock unit that holds a rootstock; and a joint part that joins the scion and the rootstock by attaching the rootstock unit to the scion unit.

IPC Classes  ?

• A01G 2/32 - Automatic apparatus therefor

Abstract

The problem addressed by the present invention is to provide a simpler, more efficient plant genome editing technique in which gene recombination is not essential. The problem is solved by a polynucleotide including a split-Cas protein coding sequence and a transitive plant virus vector sequence.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 15/83 - Viral vectors, e.g. cauliflower mosaic virus

Abstract

Provided is a member for grafting use, which enables the improvement in accuracy of grafting and the productivity of a graft seedling. The present disclosure is a member for grafting use, which is provided with: a graft unit which has a first contact part that comes in contact with a stem of a plant at a first position; a rootstock unit which is attachable and detachable to and from the graft unit and has a second contact part that comes in contact with the stem of the plant at a second position that is nearer to a root of the plant than the first position; a first fixing tool which is fixable to the graft unit and has a first pressing part at which the stem of the plant can be pressed against the first contact part; and a second fixing tool which is fixable to the rootstock unit and has a second pressing part at which the stem of the plant can be pressed against the second contact part. A space in which the stem of the plant is to be exposed is provided between the first fixing tool fixed to the graft unit and the second fixing tool fixed to the rootstock unit.

IPC Classes  ?

• A01G 2/35 - CuttingInserting

Goods & Services

Glue and adhesives for industrial purpose; glutinous preparations for tree-grafting; grafting wax for trees; grafting mastic for trees. Agricultural machines and agricultural implements, other than hand-operated; agricultural machines and agricultural implements for tree-grafting. Bladed or pointed hand tools; grafting tools [hand tools]. Stakes, not of metal, for plants or trees; stakes, not of metal, for tree-grafting. Seeds and bulbs; bulbs for horticultural purposes; seeds for horticultural purposes; bulbs for agricultural purposes; seeds for agricultural purposes; saplings; vegetables, fresh; fruits, fresh; foxtail millet, unprocessed; proso millet, unprocessed; sesame, unprocessed; buckwheat, unprocessed; corn [unprocessed grain]; flowers [natural]. Testing, inspection or research on agriculture livestock breeding or fisheries; testing, inspection or research on tree-grafting; testing or research on machines, apparatus and instruments; testing or research on machines, apparatus and instruments for tree-grafting; scientific research in the field of genetic engineering. Garden tree planting; plant nurseries; rental of potted plants; farming equipment rental.

Goods & Services

[ Bulbs for horticultural purposes; seeds for horticultural purposes; bulbs for agricultural purposes; seeds for agricultural purposes; saplings; fresh vegetables; fresh fruits; unprocessed foxtail millet; unprocessed proso millet; edible sesame, unprocessed; unprocessed buckwheat; unprocessed corn; natural flowers; all of the aforesaid in this class excluding pecans ] [ Testing, inspection or research on agriculture livestock breeding or fisheries; testing the functionality of machines, apparatus and instruments; research on machines, apparatus and instruments; ] scientific research in the field of genetic engineering [ Garden tree planting; plant nursery services; rental of potted plants; farming equipment rental ] [ Glue and adhesives for industrial purpose; grafting mastic for trees ] [ Agricultural machines and agricultural implements, other than hand-operated, namely, plows, cultivators, harvesters; fodder presses, fodder cutting machines, fodder mixing machines, cake-fodder crushing machines ] [ Hand tools, namely, grafting tools ] [ Stakes, not of metal, for plants or trees ]