An instrument for detecting a position in space may include a proof mass arranged along an input axis, an electromagnetic coil arranged at each end of the proof mass and configured to suspend the proof mass therebetween, wherein the proof mass is configured to rotate along the input axis, and at least one rotation sensor configured to detect the rotational position of the proof mass.
G01C 19/72 - Gyrometers using the Sagnac effect, i.e. rotation-induced shifts between counter-rotating electromagnetic beams with counter-rotating light beams in a passive ring, e.g. fibre laser gyrometers
An optical gimbal system includes a mirror having a non-planar surface and a digital camera having an optical axis directed toward a portion of the non-planar surface to have the mirror fold the optical axis. A motor is mechanically coupled to the mirror, the digital camera, or both the mirror and the digital camera. The motor is configured to translate the mirror relative to the digital camera along at least one translation axis.
A microthruster array system (100) includes a package housing (102) configured to receive circuitry (110) and a propellant housing (112), a nozzle plate (126) configured to close the package housing and ensure element ignition, the propellant housing arranged between the nozzle and the package housing and configured to house propellant for use in propulsion of the microthruster, and wherein the nozzle plate defines a plurality of openings (132) for controlling a flow of the propellant during propulsion.
F02K 9/14 - Shape or structure of solid propellant charges made from sheet-like materials, e.g. of carpet-roll type, of layered structure
F02K 9/30 - Rocket-engine plants, i.e. plants carrying both fuel and oxidant thereforControl thereof using solid propellants with the propulsion gases exhausting through a plurality of nozzles
F02K 9/95 - Rocket-engine plants, i.e. plants carrying both fuel and oxidant thereforControl thereof characterised by starting or ignition means or arrangements
B64G 1/26 - Guiding or controlling apparatus, e.g. for attitude control using jets
C06B 45/00 - Compositions or products which are defined by structure or arrangement of component or product
4.
SYSTEM AND PROCESS FOR THE ADDITIVE MANUFACTURING OF RF TUNABLE MATERIALS
Radio frequency (RF) tunable materials which are readily compatible with additive manufacturing techniques, such as 3D printing technologies, are provided. RF tunable materials are used to form an RF device that operates over a wide range of frequencies. The RF device is formed by interpenetrating structures of one or more composite materials. One or more curing methods are applied to the structures. The composite materials include performance materials and are be used to fabricate RF devices including RF antennas, RF horn antennas, graded index devices.
B29C 64/118 - Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using filamentary material being melted, e.g. fused deposition modelling [FDM]
A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.
A touchless system for assessing a status of a target individual may include an optical component configured to image a patient sample, a Raman spectrometer subsystem configured to detect inelastically scattered light from the patient sample, and a laser-induced fluorescence spectrometer subsystem configured to detect fluorescence emissions of the sample; and a controller configured to receive the inelastically scattered light from the Raman spectrometer subsystem, receive the fluorescence emissions from the laser-induced fluorescence spectrometer subsystem, and determine a status of the sample based on the inelastically scattered light and the fluorescence emissions.
Methods, microarrays, and kits for detecting target nucleic acid molecules (e.g., in a sample) using immobilized guide RNA (gRNA), or gRNA/Cas complexes, are provided herein. In some embodiments disclosed herein, a plurality of guide RNA (gRNA) molecules are bound, directly or indirectly, to a solid support, wherein at least one gRNA molecule in the plurality comprises a nucleotide sequence that is complementary to a portion of the target nucleic acid in the sample.
Navigation with light refraction or dispersion and landmark data is provided. A system can include a data processing system. The data processing system can receive a first image of a surface of a planet from a first camera. The data processing system can generate a first position dataset based on the first image and data representing landmarks of the surface of the planet. The data processing system can receive, by a second camera oriented towards an atmosphere of the planet, a second image. The data processing can generate, via a celestial body catalog, a second position dataset based at least in part on an amount of refraction or dispersion of light of a celestial body in a second image. The data processing system can determine, based on a filter applied to the first position dataset and the second position dataset, a position and attitude of a vehicle.
A textile conductor and a method of making the same including a textile thread, an electrical bus within the textile thread, wherein the electrical bus includes at least one top electrically conductive pad, at least one bottom electrically conductive pad, and a plurality of rows of a plurality of electrically conductive traces A dielectric material is positioned between the at least one of the at least one top electrically conductive pad, the plurality of rows of the plurality of electrically conductive traces, and the at least one bottom electrically conductive pad. At least one electrically conductive via connects at least one of the at least one top electrically conductive pad and the at least one bottom electrically conductive pad to at least one of the plurality of electrically conductive traces in at least one of the plurality of rows of the plurality of electrically conductive traces.
A system and method of processing instructions may comprise an application processing domain (APD) and a metadata processing domain (MTD). The APD may comprise an application processor executing instructions and providing related information to the MTD. The MTD may comprise a tag processing unit (TPU) having a cache of policy-based rules enforced by the MTD. The TPU may determine, based on policies being enforced and metadata tags and operands associated with the instructions, that the instructions are allowed to execute (i.e., are valid). The TPU may write, if the instructions are valid, the metadata tags to a queue. The queue may (i) receive operation output information from the application processing domain, (ii) receive, from the TPU, the metadata tags, (iii) output, responsive to receiving the metadata tags, resulting information indicative of the operation output information and the metadata tags; and (iv) permit the resulting information to be written to memory.
G06F 12/0875 - Addressing of a memory level in which the access to the desired data or data block requires associative addressing means, e.g. caches with dedicated cache, e.g. instruction or stack
G06F 12/1009 - Address translation using page tables, e.g. page table structures
G06F 12/14 - Protection against unauthorised use of memory
G06F 21/52 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure
G06F 21/57 - Certifying or maintaining trusted computer platforms, e.g. secure boots or power-downs, version controls, system software checks, secure updates or assessing vulnerabilities
G06F 21/62 - Protecting access to data via a platform, e.g. using keys or access control rules
G06F 21/71 - Protecting specific internal or peripheral components, in which the protection of a component leads to protection of the entire computer to assure secure computing or processing of information
11.
SYSTEMS AND METHODS FOR PROVIDING DYNAMIC SECURITY FABRIC INTERPOSERS IN HETEROGENEOUSLY INTEGRATED SYSTEMS
A system may include an integrated circuit (IC) package. The IC package may include one or more IC die hosting one or more circuits, and an interposer. The interposer may be coupled to the one or more IC die via an interconnection layer. The interposer may include one or more electrically active devices configured to provide one or more security functions to secure the one or more circuits. The interposer may be coupled to a backside power of the IC package. In some embodiments, the backside power of the IC package may include one or more backside power delivery networks.
H01L 23/538 - Arrangements for conducting electric current within the device in operation from one component to another the interconnection structure between a plurality of semiconductor chips being formed on, or in, insulating substrates
H01L 25/065 - Assemblies consisting of a plurality of individual semiconductor or other solid-state devices all the devices being of a type provided for in a single subclass of subclasses , , , , or , e.g. assemblies of rectifier diodes the devices not having separate containers the devices being of a type provided for in group
12.
SYNTHESIS AND INTEGRATION OF NOBLE GAS RADIOISOTOPE POWER SYSTEMS
A system to generate power in a low orbit environment may include an enclosure configured to maintain a noble gas radioisotope at a threshold pressure, wherein the noble gas radioisotope generates heat at the threshold pressure, a radiator configured to maintain the generated heat at a threshold heat, and a power converter configured to convert the maintained heat to electric power.
G21H 1/10 - Cells in which radiation heats a thermoelectric junction or a thermionic converter
G21H 3/00 - Arrangements for direct conversion of radiation energy from radioactive sources into forms of energy other than electric energy, e.g. light
According to various aspects, a sensor system is provided comprising a first substrate configured to be coupled to a user, an electric field detector to detect a user electric field and comprising a second substrate, a proof mass positioned above the second substrate, one or more electrodes coupled to the second substrate, and a control circuit coupled to the one or more electrodes, the control circuit being configured to determine a change in capacitance between the proof mass and each electrode responsive to torsional movement of the proof mass responsive to the electric field, and a controller coupled to the first substrate and being configured to receive, from the detector, information indicative of each change in capacitance between the proof mass and each electrode, and determine, based on the information, characteristics of the electric field in at least two dimensions.
Gravity map based navigation is provided. A system (100) can include a data processing system (115) including one or more processors (535), coupled with memory (515). The data processing system (115) can retrieve, based on an estimated position (175) of the system (100), a value of gravity (160) from values of gravity mapped to positions (155). The data processing system (115) can determine an altitude (187) of the system (100) based on at least the value of gravity (160) and a force measured by an accelerometer (125) of the system (100). The data processing system (115) can compare the altitude (187) with a reference altitude (165) to determine an altitude error measurement (197). The data processing system (115) can determine a lateral position of the system (100) based on the altitude error measurement (197).
G01C 21/16 - NavigationNavigational instruments not provided for in groups by using measurement of speed or acceleration executed aboard the object being navigatedDead reckoning by integrating acceleration or speed, i.e. inertial navigation
15.
Multiple Effective Focal Length (EFL) Optical System
A multi-band/multi-polarization reflective or catadioptric optical system yields differing effective focal lengths (EFLs) per band/polarization. This approach could be used to create an imaging system, for example. In such case, a sensor (imager, spectrometer, diode, etc.) is located at the one or more focal planes. On the other hand, it could also be used to create a projecting system or hybrid projecting and imaging system by locating an emitter such as an LED, laser, etc.) at the image or focal plane. The system employs polarizers and/or dichroic coatings nano patterns to create different focal lengths and/or fields of view using the same mirrors and/or lenses by, for example, including at least one dichroic coating optically in front of at least one additional mirror to separately reflect the different bands or polarizations.
A method of printing patterned tissue includes flowing a bioink comprising cells and a biological cargo through a nozzle moving relative to a substrate and exposing selected voxels of the bioink to a pulsed electric field as the bioink flows through the nozzle. Consequently, a portion or all of the cells in each of the selected voxels undergoes electroporation and transfection with the biological cargo. A cell-laden filament comprising the bioink and including the selected voxels is continuously extruded from an outlet of the nozzle, and, as the nozzle moves relative to the substrate, the cell-laden filament is deposited in a predetermined pattern on the substrate. Thus, a tissue having spatial patterns of gene expression may be printed.
A61L 27/54 - Biologically active materials, e.g. therapeutic substances
B41J 2/005 - Typewriters or selective printing mechanisms characterised by the printing or marking process for which they are designed characterised by bringing liquid or particles selectively into contact with a printing material
An optical sensor system for communication and pointing may include an optical sensor having photocells arranged in an array configured to receive at least one optical signal, and the optical sensor, in response to the optical signal, generating a first output signal and a second output signal, wherein the first output signal represents instantaneous photon flux of the received optical signal and the second output signal represents a derivative of the instantaneous photon flux of the received optical signal where the optical sensor operates as a dual communication and pointing sensor.
A micro heat engine for utilizing temperature differential for high efficiency heat conversion, may include a housing have a hot side and a cold side, a plurality of pistons arranged within the housing, each extending from the hot side to the cold side; and a plurality of regenerators, each configured to be arranged between two adjacent pistons and configured to store heat generated as working gas moves within the adjacent pistons.
F02G 1/043 - Hot gas positive-displacement engine plants of closed-cycle type the engine being operated by expansion and contraction of a mass of working gas which is heated and cooled in one of a plurality of constantly communicating expansible chambers, e.g. Stirling cycle type engines
A control circuit for an electrostatic machine includes a current source or sink, an inductor, a switching network coupled between the current source or sink and the electrostatic machine, and between the inductor and the electrostatic machine. A controller is configured to automatically cause the switching network to connect the current source or sink to the electrostatic machine each half-cycle of a periodically alternating polarity. At each polarity alternation, the electrostatic machine is isolated from the current source or sink for a first period of time. At each polarity alternation, the inductor is connected to the electrostatic machine for a second period of time while the electrostatic machine is isolated from the current source or sink, and then disconnect the inductor from the electrostatic machine.
A method for reconfiguring data referenced during the execution of a software system without the need for prior knowledge of a structured memory layout of the software system, may include parsing qualified software identifiers from the software system and categorizing the qualified software identifiers as either functions or variables, each associated with a name, data type, and data source, correlating the qualified software identifiers with at least one constraint defined by the categorizing, generating an order of execution of the qualified software identifiers based on the constraint, executing tasks defined by the software system according to the order of the qualified software identifiers, in response to a need arising during the execution of the software system, reconfiguring of the order of execution of the qualified software identifiers, and executing tasks defined by the reconfiguration of the order of the qualified software identifiers.
A LiDAR system includes a light source and an arrayed micro-optic configured to receive light from the light source so as to produce and project a two-dimensional array of light spots on a scene. The LiDAR system also includes receiver optics having an array of optical detection sites configured so as to be suitable for establishing a one-to-one correspondence between light spots in the two-dimensional array and optical detection sites in the receiver optics. The LiDAR system further includes a birefringent prism and a lens. The LiDAR system may also include a mask placed in the light path between the birefringent prism and the receiver optics. Alternatively, the LiDAR system may include a controller programmed to activate or deactivate each optical detection site.
An antenna system has a two-dimensional field of view, yet can be implemented on a surface, such as on electronic or photonic integrated circuits. The antenna system includes an array of antennas disposed in a predetermined non-linear pattern and a two-dimensional beamforming network (BFN). The antenna system can be steered/selectively beamformed in two dimensions through beam port selection. The beamforming network is disposed entirely on a single first surface. The beamforming network has a one-dimensional array-side interface disposed on the first surface and a one-dimensional beam-side interface disposed on the first surface. The antennas of the array of antennas are individually communicably coupled to the array-side interface. Segments of the beam-side interface map to respective pixels in the two-dimensional field of view.
H01Q 3/40 - Arrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the relative phase or relative amplitude of energisation between two or more active radiating elementsArrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the distribution of energy across a radiating aperture varying the phase by electrical means with phasing matrix
23.
FABRICATION METHOD FOR COMPLEX, RE-ENTRANT 3D MICROSCALE STRUCTURES
A bilayer mold for forming polymer microstructures is provided. The bilayer mold includes a rigid plastic cylinder having slots defined along an outer periphery of the rigid plastic cylinder; and a layer of plastic elastic polymer material disposed within the slots and having indents defined therethrough and in the rigid plastic cylinder. The indents have a first profile extending through the layer of plastic elastic polymer and terminating within the rigid plastic cylinder. A metal polymer structure profile is also provided using laser-etching of a desired shape for the features of the microstructures in a metallic foil, followed by further refining of the features. The polymer and metal microstructures can be formed on the surface of a stent to facilitate retention of the stent.
A method of lidar imaging pulses a scene with laser pulse sequences from a laser light source. Reflected light from the scene is measured for each laser pulse to form a sequence of time resolved light signals. Adjoining time bins in the time resolved light signals are combined to form super time bins. A three dimensional image of the scene is created from distances determined based on maximum intensity super time bins. One or more objects are located within the image. For each object, the time resolved light signals are combined to form a single object time resolved light signal from which to determine distance to the object.
A device, system and process involve conducting electroporation of microvesicles or exosomes or other target structures in a microfluidic arrangement at pressures that exceed atmospheric pressure. Single as well as multiple flow configurations can be employed. In some cases, the system and its operation are computer-controlled for partial or complete automation.
A system and method of LIDAR imaging to overcome scattering effects pulses a scene with light pulse sequences from a light source. Reflected light from the scene is measured for each light pulse to form a sequence of time-resolved signals. Time-resolved contrast is calculated for each location in a scene. A three-dimensional map or image of the scene is created from the time-resolved contrasts. The three-dimensional map is then utilized to affect operation of a vehicle.
Systems and apparatuses for blood oxygenation are disclosed. A system includes a first layer defining a plurality of banks of first channels each extending in a first direction. The plurality of banks of first channels are configured to receive blood via a trunk channel. The system includes a second layer defining a bank of second channels extending in a second direction. The bank of second channels are configured to receive oxygen. The first direction is different from the second direction. The system includes a membrane disposed between the first layer and the second layer and configured to cause the oxygen to permeate from the second layer to the first layer to oxygenate the blood.
Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.
A system and processes enable determination of the genetic location of a sequence of interest including markers of genetic engineering. More specifically, a system and/or a series of processes are used independently or together to allow assembly, annotation, and visualization of genetic elements of interest (GEI) as well as their genetic surroundings from sequencing data. This can enable users to quickly and efficiently review and interpret the results so they can understand what GEIs are present in a sample, where they are, and their surroundings. It can be used to enrich sequences of interest or with sequences that have not been enriched and can help answer research questions such as understanding the on and off target effects of genetic engineering or discovery of novel gene homologs.
Miniaturized DNA microarrays are described to be used in conjunction with microfluidic channels or microcentrifuge tubes and microcentrifuge filters to reduce sample size, incubation time and to increase overall binding efficiency.
B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
B01L 7/00 - Heating or cooling apparatusHeat insulating devices
B04B 3/00 - Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneously sifting or filtering
B04B 5/04 - Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
31.
ACOUSTIC SEPARATION OF PARTICLES FOR BIOPROCESSING
A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
Provided herein are engineered terminal deoxynucleotidyl transferase (TdT) proteins with certain modifications, including mutations to confer thermal stability and to install an exposed amino acid residue to which a small molecule can be covalently tethered via bioconjugate chemistries such as click chemistry. Also provided herein are methods of nucleic acid molecule synthesis using engineered TdTs and nucleotide molecules attached to redox-cleavable linkers, wherein the engineered TdT incorporates the nucleotide molecule into a nucleic acid strand and is separated from the nucleotide molecule when the redoxcleavable linker is cleaved upon exposure to suitable electrochemical conditions. Also provided herein are engineered TdTs covalently attached to a nucleotide molecule via a tether and also nucleotide molecules comprising a redox-cleavable linker. Further provided herein are systems for enzymatic DNA synthesis comprising an engineered TdT, a redox-cleavable linker a redox shuttle solution, and two or more electrodes.
Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.
A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.
G02B 13/14 - Optical objectives specially designed for the purposes specified below for use with infrared or ultraviolet radiation
C23C 16/04 - Coating on selected surface areas, e.g. using masks
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
G02B 1/00 - Optical elements characterised by the material of which they are madeOptical coatings for optical elements
G03F 7/00 - Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printed surfacesMaterials therefor, e.g. comprising photoresistsApparatus specially adapted therefor
G03F 7/40 - Treatment after imagewise removal, e.g. baking
H01L 31/02 - SEMICONDUCTOR DEVICES NOT COVERED BY CLASS - Details thereof - Details
35.
MICROFLUIDIC TISSUE BIOPSY AND IMMUNE RESPONSE DRUG EVALUATION DEVICES AND SYSTEMS
This disclosure describes microfluidic tissue biopsy and immune response drug evaluation devices and systems. A microfluidic device can include an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample. The microfluidic device can also include a tissue trapping region at the second end of the inlet channel downstream from the first end. The tissue trapping region can include one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the tissue trap. The microfluidic device can also include one or more channels providing an outlet.
A distributed navigation system includes navigation platforms, each having a universal navigation processor, relative navigation systems to provide source information to the navigation platforms, navigation filters provided on one or more of the universal navigation processors, and an anchor navigation node disposed on one or more of the navigation platforms to form one or more anchor navigation platforms. Each anchor navigation node includes an inertial navigation system, a clock, and absolute navigation systems, which are used, in combination with source information, to determine navigation information. The anchor navigation platforms provide the navigation information to other navigation platforms. The system further includes a navigation processor system in communication with the universal navigation processors to provide operating information updates to the universal navigation processors and a graphical user interface to display the navigation information to a user and permit the user to review and control the navigation information.
G01S 19/47 - Determining position by combining measurements of signals from the satellite radio beacon positioning system with a supplementary measurement the supplementary measurement being an inertial measurement, e.g. tightly coupled inertial
G01C 21/16 - NavigationNavigational instruments not provided for in groups by using measurement of speed or acceleration executed aboard the object being navigatedDead reckoning by integrating acceleration or speed, i.e. inertial navigation
G01C 21/28 - NavigationNavigational instruments not provided for in groups specially adapted for navigation in a road network with correlation of data from several navigational instruments
G01S 5/00 - Position-fixing by co-ordinating two or more direction or position-line determinationsPosition-fixing by co-ordinating two or more distance determinations
G01S 19/05 - Cooperating elementsInteraction or communication between different cooperating elements or between cooperating elements and receivers providing aiding data
37.
Methods and Systems for Extraction, Processing, and Detection of Nucleic Acids
A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.
The present disclosure provides methods of generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a linear bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.
A system and method of processing instructions may comprise an application processing domain (APD) and a metadata processing domain (MTD). The APD may comprise an application processor executing instructions and providing related information to the MTD. The MTD may comprise a tag processing unit (TPU) having a cache of policy-based rules enforced by the MTD. The TPU may determine, based on policies being enforced and metadata tags and operands associated with the instructions, that the instructions are allowed to execute (i.e., are valid). The TPU may write, if the instructions are valid, the metadata tags to a queue. The queue may (i) receive operation output information from the application processing domain, (ii) receive, from the TPU, the metadata tags, (iii) output, responsive to receiving the metadata tags, resulting information indicative of the operation output information and the metadata tags; and (iv) permit the resulting information to be written to memory.
G06F 9/38 - Concurrent instruction execution, e.g. pipeline or look ahead
G06F 9/30 - Arrangements for executing machine instructions, e.g. instruction decode
G06F 12/1009 - Address translation using page tables, e.g. page table structures
G06F 12/14 - Protection against unauthorised use of memory
G06F 21/52 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure
G06F 21/57 - Certifying or maintaining trusted computer platforms, e.g. secure boots or power-downs, version controls, system software checks, secure updates or assessing vulnerabilities
G06F 21/62 - Protecting access to data via a platform, e.g. using keys or access control rules
G06F 21/71 - Protecting specific internal or peripheral components, in which the protection of a component leads to protection of the entire computer to assure secure computing or processing of information
G06F 12/0875 - Addressing of a memory level in which the access to the desired data or data block requires associative addressing means, e.g. caches with dedicated cache, e.g. instruction or stack
A method for generating a cell support interface for use in a three dimensional cell culture environment, may include electrospinning a mat having an epithelial support layer configured to create an intimate coupling between the epithelial cell and a porous matrix, including a first layer and a second layer, wherein the first layer is formed using a first solution at a first viscosity level and the second layer is formed using a second solution at a second viscosity level different from the first viscosity level.
A distributed navigation system includes navigation platforms, each having a universal navigation processor, relative navigation systems to provide source information to the navigation platforms, navigation filters provided on one or more of the universal navigation processors, and an anchor navigation node disposed on one or more of the navigation platforms to form one or more anchor navigation platforms. Each anchor navigation node includes an inertial navigation system, a clock, and absolute navigation systems, which are used, in combination with source information, to determine navigation information. The anchor navigation platforms provide the navigation information to other navigation platforms. The system further includes a navigation processor system in communication with the universal navigation processors to provide operating information updates to the universal navigation processors and a graphical user interface to display the navigation information to a user and permit the user to review and control the navigation information.
G01S 19/47 - Determining position by combining measurements of signals from the satellite radio beacon positioning system with a supplementary measurement the supplementary measurement being an inertial measurement, e.g. tightly coupled inertial
G01C 21/28 - NavigationNavigational instruments not provided for in groups specially adapted for navigation in a road network with correlation of data from several navigational instruments
G01C 21/16 - NavigationNavigational instruments not provided for in groups by using measurement of speed or acceleration executed aboard the object being navigatedDead reckoning by integrating acceleration or speed, i.e. inertial navigation
G01S 19/05 - Cooperating elementsInteraction or communication between different cooperating elements or between cooperating elements and receivers providing aiding data
G01S 5/00 - Position-fixing by co-ordinating two or more direction or position-line determinationsPosition-fixing by co-ordinating two or more distance determinations
43.
FAST WELL PLATE DIFFERENTIAL SCANNING MICRO-CALORIMETER USING PHOTONIC SENSORS
A system for calorimetry includes a plurality of wells disposed upon a well plate, an input feature to deposit a sample within each well, and light sources configurable to irradiate each of the wells in the well plate, and their samples, with incident light. A photonic sensor chip at a bottom of each well includes a plural nanohole array sensor on a substrate. A light detector positioned below the well is configured to measure the transmission of light through the sensors, obtaining a series of optical transmission measurements. A heater is in thermal contact with each of the wells, applying a transient thermal increase to each well, and the sample therein, at a known heat rate. A processor is configured to calculate a measurement for each well as a function of the series of optical transmission measurements and the transient thermal increase, the measurement being indicative of the sample within the well undergoing a change in response to the transient thermal increase, the change relating to a property of the sample.
G01N 25/48 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on solution, sorption, or a chemical reaction not involving combustion or catalytic oxidation
According to various aspects and embodiments, a growth adaptive expandable stent is provided. The expandable stent includes a stent structure having a cylindrical shape that is self-expanding in a radial direction and includes a plurality of cylindrical rings disposed along a longitudinal axis of the stent structure. The stent structure is configured to exert a continuous outward radial force over time when implanted such that a diameter of the stent structure expands from a first value to a second value that is at least about 1.5 times the first value.
A61F 2/89 - Stents in a form characterised by wire-like elementsStents in a form characterised by a net-like or mesh-like structure the wire-like elements comprising two or more adjacent rings flexibly connected by separate members
A tail for a projectile includes a body having a longitudinal axis. A steering assembly is secured to the body. The steering assembly includes a flap movable from a first position in which the flap does not extend radially beyond the body to a second position in which the flap extends radially beyond the body and at an angle relative to the longitudinal axis, and a flap release mechanism. A projectile including a tail according to the present disclosure is also provided.
A system can include a balloon catheter, a pump, a pressure sensor, and one or more processors, The balloon catheter can be configured to be inserted into a lumen of a tissue. The pump can be configured to provide fluid into the balloon catheter to cause the balloon catheter to induce a radial stress of the tissue. The pressure sensor can be configured to detect a pressure of the fluid while the balloon catheter is inducing the radial stress of the tissue. The one or more processors can be configured to determine a characteristic of the tissue based at least on the pressure.
A method of manufacturing synthetic particles for use in microfluidic devices is disclosed. The method includes identifying a set of particle characteristics for a fluid-based process. The set of particle characteristics can include a synthetic particle density and one or more of a size, compressibility, elastic modulus, or porosity. The method includes selecting an input material for the synthetic particles based on the set of synthetic particle characteristics. The method may include selecting an additive based on the set of synthetic particle characteristics. The method includes providing input material and the additive into a droplet generator to create one or more synthetic particles having the set of synthetic particle characteristics, and modifying a surface characteristic the synthetic particles, such that the synthetic particles bind to a target particle in a solution.
A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.
Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating undesirable particles bound to capture particles from formed elements of whole blood. After introducing the capture particles to whole blood containing undesirable particles, the whole blood and capture particles are flowed through a microfluidic separation channel. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements and undesirable particles bound to capture particles to specific aggregation axes. After aggregating the particles, the formed elements exit the separation channel through a first outlet and are returned to the patient. The undesirable particles, bound to the capture particles, exit through a second outlet and can be discarded to saved for later study.
The present disclosure describes a system for the delivery of therapeutic substances to the cavities of a patient. The system can include a wearable micropump that is fluidically coupled with a handpiece. The handpiece can be inserted, for example, into the middle ear via a surgical tympanotomy approach. The system can enable a controlled injection of a therapeutic substance directly into the patient's cavity.
Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.
An embodiment is directed to a hardware circuit for encrypting and/or decrypting data transmitted between a processor and a memory. The circuit is situated between the processor and memory. The circuit includes a first interface communicatively coupled to the processor via a set of buses. The circuit also includes a second interface communicatively coupled to the memory. The circuit further includes hardware logic capable of executing an encryption operation on data transmitted between the processor and memory, without adding latency to data transmission speed between the processor and the memory. The hardware logic is configured to encrypt data received at the first interface from the processor, and transmit the encrypted data to the memory via the second interface. The hardware logic is also configured to decrypt data received at the second interface from the memory, and transmit the decrypted data to the processor via the first interface.
G06F 21/78 - Protecting specific internal or peripheral components, in which the protection of a component leads to protection of the entire computer to assure secure storage of data
53.
Method and system for control and readout of tuning fork gyroscope
A tuning fork sensor system places a controlled bias on the proof-mass drive-axis electrodes to cancel the quadrature charge. Also, its charge amplifiers employ a field-effect transistor biased slightly into the triode region so that it behaves as a very large value resistor. In addition, it uses a phase-locked loop having a special loop filter in order to optimize performance by rejecting off-frequency drive feedthrough to the motor pick-off while resulting in very low phase wander for the demodulation references.
G01C 19/5607 - Turn-sensitive devices using vibrating masses, e.g. vibratory angular rate sensors based on Coriolis forces using vibrating tuning forks
H03F 1/08 - Modifications of amplifiers to reduce detrimental influences of internal impedances of amplifying elements
54.
METHOD OF ACOUSTOPHORESIS USING SELECTION PARTICLES THAT ALTER ACOUSTIC RESPONSE AND CORRELATED SYSTEM
A method of acoustophoresis using selection particles that alter acoustic response is provided. The method can include selecting a set of selection particles based on surface markers of a plurality of target particles to be separated using acoustophoresis. The method can include incubating the set of selection particles with the plurality of target particles in a solution such that the set of selection particles bind with the surface markers on the plurality of target particles to create a plurality of bound particles. The method can include providing the plurality of bound particles to an acoustophoresis device tuned to separate the particles based on a net acoustic contrast between each of the plurality of bound particles. The method can include receiving a plurality of output streams from the acoustophoresis device that each include a respective bound particle of the plurality of bound particles.
An electromagnetic gradiometer that includes multiple torsionally operated MEMS-based magnetic and/or electric field sensors with control electronics configured to provide magnetic and/or electric field gradient measurements. In one example a magnetic gradiometer includes a first torsionally operated MEMS magnetic sensor having a capacitive read-out configured to provide a first measurement of a received magnetic field, a second torsionally operated MEMS magnetic sensor coupled to the first torsionally operated MEMS magnetic sensor and having the capacitive read-out configured to provide a second measurement of the received magnetic field, and control electronics coupled to the first and second torsionally operated MEMS magnetic sensors and configured to determine a magnetic field gradient of the received magnetic field based the first and second measurements from the first and second torsionally operated MEMS electromagnetic sensors.
A method of acoustophoresis using selection particles that alter acoustic response is provided. The method can include selecting a set of selection particles based on surface markers of a plurality of target particles to be separated using acoustophoresis. The method can include incubating the set of selection particles with the plurality of target particles in a solution such that the set of selection particles bind with the surface markers on the plurality of target particles to create a plurality of bound particles. The method can include providing the plurality of bound particles to an acoustophoresis device tuned to separate the particles based on a net acoustic contrast between each of the plurality of bound particles. The method can include receiving a plurality of output streams from the acoustophoresis device that each include a respective bound particle of the plurality of bound particles.
An extracorporeal blood treatment module includes a plurality of gas transfer units, having a first polymer layer with a plurality of gas channels, a second polymer layer with a plurality of blood channels, and a gas permeable membrane disposed between the plurality of gas channels and the plurality of blood channels, a fluid transfer unit integrated with the plurality of gas transfer units, and including a third polymer layer having a plurality of fluid collection channels, a fourth polymer layer having a plurality of blood channels, and a fluid permeable membrane disposed between the plurality of fluid collection channels and the plurality of blood channels, and a housing containing the plurality of gas transfer units and fluid transfer unit.
A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
A LiDAR system includes a light source and an arrayed micro-optic configured to receive light from the light source so as to produce and project a two-dimensional array of light spots on a scene. The LiDAR system also includes receiver optics having an array of optical detection sites configured so as to be suitable for establishing a one-to-one correspondence between light spots in the two-dimensional array and optical detection sites in the receiver optics. The LiDAR system further includes a birefringent prism and a lens. The LiDAR system may also include a mask placed in the light path between the birefringent prism and the receiver optics. Alternatively, the LiDAR system may include a controller programmed to activate or deactivate each optical detection site.
Methods for fabricating MEMS tuning fork gyroscope sensor system using silicon wafers. This provides the possibly to avoid glass. The sense plates can be formed in a device layer of a silicon on insulator (SOI) wafer or in a deposited polysilicon layer in a few examples.
G01C 19/5621 - Turn-sensitive devices using vibrating masses, e.g. vibratory angular rate sensors based on Coriolis forces using vibrating tuning forks the devices involving a micromechanical structure
B81B 3/00 - Devices comprising flexible or deformable elements, e.g. comprising elastic tongues or membranes
B81C 1/00 - Manufacture or treatment of devices or systems in or on a substrate
Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.
A system and method of LIDAR imaging to overcome scattering effects pulses a scene with light pulse sequences from a light source. Reflected light from the scene is measured for each light pulse to form a sequence of time-resolved signals. Time-resolved contrast is calculated for each location in a scene. A three-dimensional map or image of the scene is created from the time-resolved contrasts. The three-dimensional map is then utilized to affect operation of a vehicle.
A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
C12M 1/00 - Apparatus for enzymology or microbiology
C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/40 - Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.
A Fano resonator device can be used to sequence DNA or other macromolecules. The device includes customized molecular components, informed by computation analysis. Techniques for preparing and using the device also are disclosed. The device can be incorporated in a system that further includes a sample processing component and a flow chamber.
A Fano resonator device can be used to sequence DNA or other macromolecules. The device includes customized molecular components, informed by computation analysis. Techniques for preparing and using the device also are disclosed. The device can be incorporated in a system that further includes a sample processing component and a flow chamber.
G01N 27/414 - Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
B82Y 15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
Devices for treatment of cells are disclosed. The devices include an elongated housing and at least one hollow fiber semi-permeable membrane positioned within the housing having a plurality of pores dimensioned to prevent passage of the cells to be treated. Systems for treatment of cells including the device are disclosed. Methods of treating cells, including transducing cells and activating cells, are also disclosed. The methods include introducing a biosample with cells to be treated into the device, introducing media to suspend and release treated cells into the device, and discharging the treated cells from the device.
The present solution provides trans-round window membrane (RWM) drug delivery. As an overview, the system can include a micropump that is connected to a flexible cannula. The cannula can include a stiffened and sharpened tip to facilitate insertion through the RWM. The cannula can be inserted through the RWM to improve the distribution of the delivered drug throughout the inner ear. The present solution can function as a small implantable or wearable device that can be used for both chronic and acute trans-RWM drug delivery. With this configuration, the micropump can constantly or intermittently deliver, over a period of days to months, small volumes of drugs from an internal reservoir.
A61M 5/145 - Pressure infusion, e.g. using pumps using pressurised reservoirs, e.g. by means of pistons
A61F 11/00 - Methods or devices for treatment of the ears or hearing sense Non-electric hearing aidsMethods or devices for enabling ear patients to achieve auditory perception through physiological senses other than hearing senseProtective devices for the ears, carried on the body or in the hand
A61M 1/00 - Suction or pumping devices for medical purposesDevices for carrying-off, for treatment of, or for carrying-over, body-liquidsDrainage systems
69.
MICROSTRUCTURES FOR LONG-TERM MECHANICAL ADHESION TO TISSUE
A biocompatible adhesive is disclosed. The biocompatible adhesive includes a substrate and a plurality of micro-scale elements extending from a surface of the substrate having a length selected to puncture a layer of a target tissue or target material. At least some of the micro-scale elements include at least one protrusion dimensioned to anchor the biocompatible adhesive to the target tissue or target material. A medical device assembly is also disclosed. The medical device assembly includes the biocompatible adhesive coupled to a surface of a component of the medical device assembly and positioned to attach the medical device assembly to the target tissue or target material. A method of facilitating attachment of a medical device assembly to a target tissue is also disclosed. A method of facilitating treatment of a wound is also disclosed.
A biocompatible adhesive is disclosed. The biocompatible adhesive includes a substrate and a plurality of micro-scale elements extending from a surface of the substrate having a length selected to puncture a layer of a target tissue or target material. At least some of the micro-scale elements include at least one protrusion dimensioned to anchor the biocompatible adhesive to the target tissue or target material. A medical device assembly is also disclosed. The medical device assembly includes the biocompatible adhesive coupled to a surface of a component of the medical device assembly and positioned to attach the medical device assembly to the target tissue or target material. A method of facilitating attachment of a medical device assembly to a target tissue is also disclosed. A method of facilitating treatment of a wound is also disclosed.
A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
72.
ACOUSTIC SEPARATION FOR HIGH-SPECIFICITY PURIFICATION
A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.
A61K 35/17 - LymphocytesB-cellsT-cellsNatural killer cellsInterferon-activated or cytokine-activated lymphocytes
B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
73.
ACOUSTIC SEPARATION FOR HIGH-SPECIFICITY PURIFICATION
A method for separating cells in a biofluid includes pretreating the biofluid by introducing a predetermined amount of a cocktail of antibodies, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the pretreated biofluid within the microfluidic separation channel. A system for microfluidic cell separation, capable of separating target cells from non-target cells in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of an additive including the cocktail of antibodies, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic cell separation is also disclosed. A method of facilitating separation of cells is also disclosed.
A prosthetic device includes a closed loop system for maintaining a predetermined concentration of a target ion in a region in proximity to a cell, such as a nerve cell. The device includes a controller and an ion-selective electrode assembly operatively connected to the controller, wherein the ion-selective electrode configuration is configured to sense the concentration of the target ion by potentiometric measurement and to convey the concentration to the controller. The controller is configured to modulate the current to the ion-selective electrode assembly based on the concentration of the target ion to control the concentration of the target ion so as to maintain the predetermined concentration of the target ion.
The systems and methods of the present disclosure provide highly deformable porous membrane culture systems and actuation methods for studying the effects of biomedical stretch on cultured tissue. A well plate can include a well having a first opening configured to receive an insert coupled to a deformable membrane. The well plate can include a gasket positioned within the well and configured to create a seal between the insert and the well when the insert is positioned in the well. The well plate can include a chamber defined beneath the well, the chamber configured to receive fluid media and to expose the fluid media to a surface of the deformable membrane when the insert is positioned in the well. The well plate can include an actuator configured to stretch the deformable membrane by a target amount of strain.
The systems and methods of the present disclosure provide highly deformable porous membrane culture systems and actuation methods for studying the effects of biomedical stretch on cultured tissue. A well plate can include a well having a first opening configured to receive an insert coupled to a deformable membrane. The well plate can include a gasket positioned within the well and configured to create a seal between the insert and the well when the insert is positioned in the well. The well plate can include a chamber defined beneath the well, the chamber configured to receive fluid media and to expose the fluid media to a surface of the deformable membrane when the insert is positioned in the well. The well plate can include an actuator configured to stretch the deformable membrane by a target amount of strain.
A system for calorimetry includes a well having a volume for receiving a sample, an input feature to facilitate reception of the sample in the well, a light source to irradiate the well and the sample with incident light, and a photonic sensor chip disposed at the bottom of the well. The photonic sensor chip includes plural nanohole array (NHA) sensors. A light detector is configured to measure transmission of light through the NHA sensors to obtain a series of extraordinary optical transmission (EOT) measurements. Frame elements secure and mutually couple the light source, the photonic sensor chip, the light detector, and the input feature to form a calorimetry unit. A processor is configured to calculate a calorimetry measurement as a function of the series of EOT measurements, the calorimetry measurement being indicative of energy released as a result of the sample in the well undergoing a change.
G01N 25/20 - Investigating or analysing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
G01N 1/44 - Sample treatment involving radiation, e.g. heat
78.
System and method for translating mapping policy into code
A system including at least one processor programmed to translate a policy into policy code, wherein: the policy is provided in a policy language; the policy code is in a programming language that is different from the policy language; and the policy includes a statement that maps an entity name to one or more metadata symbols to be associated with an entity in a target system against which the policy is to be enforced.
G06F 21/51 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems at application loading time, e.g. accepting, rejecting, starting or inhibiting executable software based on integrity or source reliability
G06F 21/52 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure
G06F 21/54 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure by adding security routines or objects to programs
H04L 9/32 - Arrangements for secret or secure communicationsNetwork security protocols including means for verifying the identity or authority of a user of the system
79.
ACTUATION OF MICROCHANNELS FOR OPTIMIZED ACOUSTOPHORESIS
The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.
A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.
G02B 1/00 - Optical elements characterised by the material of which they are madeOptical coatings for optical elements
G02B 1/02 - Optical elements characterised by the material of which they are madeOptical coatings for optical elements made of crystals, e.g. rock-salt, semiconductors
G02B 13/14 - Optical objectives specially designed for the purposes specified below for use with infrared or ultraviolet radiation
H01L 31/02 - SEMICONDUCTOR DEVICES NOT COVERED BY CLASS - Details thereof - Details
G03F 7/00 - Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printed surfacesMaterials therefor, e.g. comprising photoresistsApparatus specially adapted therefor
G03F 7/40 - Treatment after imagewise removal, e.g. baking
C23C 16/455 - Chemical coating by decomposition of gaseous compounds, without leaving reaction products of surface material in the coating, i.e. chemical vapour deposition [CVD] processes characterised by the method of coating characterised by the method used for introducing gases into the reaction chamber or for modifying gas flows in the reaction chamber
C23C 16/04 - Coating on selected surface areas, e.g. using masks
81.
ACTUATION OF MICROCHANNELS FOR OPTIMIZED ACOUSTOPHORESIS
The systems and methods of the present disclosure provide techniques for the design and use of an intermediate or transitional plate or block designed to couple acoustic energy at a given frequency from a transducer, such as a piezoelectric transducer, to one or more acoustophoretic devices, such as microfluidic channels, such that driving the chip occurs with a controlled wavelength and symmetry. Such techniques provide improved efficiency when driving a single acoustophoretic device, or for multiple acoustophoretic devices to be operated in concert from a single transducer, and therefore without complex electronics. Additionally, the techniques described herein allow for relaxed design constraints when considering transducer selection and fabrication, instead transferring design constraints to the more easily customized actuation plate.
B06B 1/06 - Processes or apparatus for generating mechanical vibrations of infrasonic, sonic or ultrasonic frequency making use of electrical energy operating with piezoelectric effect or with electrostriction
B81B 1/00 - Devices without movable or flexible elements, e.g. microcapillary devices
A microchannel cell culture device is disclosed. The microchannel cell culture device includes a well plate defining an array of tissue modeling environments. A cell culture system including the microchannel cell culture device is also disclosed. The cell culture system includes a plurality of optical sensors, a platform, and a light source. A method of high throughput screening cell biological activity with the microchannel cell culture device is disclosed. A method of measuring oxygen consumption rate of cells in the microchannel cell culture device is disclosed. A method of facilitating drug development with the microchannel cell culture device is also disclosed.
A microchannel cell culture device is disclosed. The microchannel cell culture device includes a well plate defining an array of tissue modeling environments. A cell culture system including the microchannel cell culture device is also disclosed. The cell culture system includes a plurality of optical sensors, a platform, and a light source. A method of high throughput screening cell biological activity with the microchannel cell culture device is disclosed. A method of measuring oxygen consumption rate of cells in the microchannel cell culture device is disclosed. A method of facilitating drug development with the microchannel cell culture device is also disclosed.
This disclosure describes microfluidic tissue biopsy and immune response drug evaluation devices and systems. A microfluidic device can include an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample. The microfluidic device can also include a tissue trapping region at the second end of the inlet channel downstream from the first end. The tissue trapping region can include one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the tissue trap. The microfluidic device can also include one or more channels providing an outlet.
An embodiment is directed to a hardware circuit for performing operations on data transmitted between a processor and memory. The hardware circuit includes a first interface communicatively coupled to the processor. The first interface configured to emulate a first protocol of the memory. The hardware circuit further includes a second interface communicatively coupled to the memory. The second interface configured to emulates a second protocol of the processor. The hardware circuit also includes hardware logic configured with a bi-directional path, such that each of the first and second interfaces is associated with a different direction of the bi-directional path. The bi-directional path is configured to execute an operation on data received at both the first interface and the second interface.
G06F 21/00 - Security arrangements for protecting computers, components thereof, programs or data against unauthorised activity
G06F 21/79 - Protecting specific internal or peripheral components, in which the protection of a component leads to protection of the entire computer to assure secure storage of data in semiconductor storage media, e.g. directly-addressable memories
G06F 9/455 - EmulationInterpretationSoftware simulation, e.g. virtualisation or emulation of application or operating system execution engines
G06F 21/82 - Protecting input, output or interconnection devices
An antenna system has a two-dimensional field of view, yet can be implemented on a surface, such as on electronic or photonic integrated circuits. The antenna system includes an array of antennas disposed in a predetermined non-linear pattern and a two-dimensional beamforming network (BFN). The antenna system can be steered/selectively beamformed in two dimensions through beam port selection. The beamforming network is disposed entirely on a single first surface. The beamforming network has a one-dimensional array-side interface disposed on the first surface and a one-dimensional beam-side interface disposed on the first surface. The antennas of the array of antennas are individually communicably coupled to the array-side interface. Segments of the beam-side interface map to respective pixels in the two-dimensional field of view.
H01Q 21/12 - Parallel arrangements of substantially straight elongated conductive units
H01Q 1/24 - SupportsMounting means by structural association with other equipment or articles with receiving set
H01Q 3/40 - Arrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the relative phase or relative amplitude of energisation between two or more active radiating elementsArrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the distribution of energy across a radiating aperture varying the phase by electrical means with phasing matrix
H01Q 11/14 - Resonant antennas with parts bent, folded, shaped or screened or with phasing impedances, to obtain desired phase relation of radiation from selected sections of the antenna or to obtain desired polarisation effects
87.
TWO-DIMENSIONAL PLANAR AND CROSSOVER-FREE BEAMFORMING NETWORK ARCHITECTURE
An antenna system has a two-dimensional field of view, yet can be implemented on a surface, such as on electronic or photonic integrated circuits. The antenna system includes an array of antennas disposed in a predetermined non-linear pattern and a two-dimensional beamforming network (BFN). The antenna system can be steered/selectively beamformed in two dimensions through beam port selection. The beamforming network is disposed entirely on a single first surface. The beamforming network has a one-dimensional array-side interface disposed on the first surface and a one-dimensional beam-side interface disposed on the first surface. The antennas of the array of antennas are individually communicably coupled to the array-side interface. Segments of the beam-side interface map to respective pixels in the two-dimensional field of view.
H01Q 3/40 - Arrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the relative phase or relative amplitude of energisation between two or more active radiating elementsArrangements for changing or varying the orientation or the shape of the directional pattern of the waves radiated from an antenna or antenna system varying the distribution of energy across a radiating aperture varying the phase by electrical means with phasing matrix
H01Q 21/06 - Arrays of individually energised antenna units similarly polarised and spaced apart
H01Q 25/00 - Antennas or antenna systems providing at least two radiating patterns
H04B 7/06 - Diversity systemsMulti-antenna systems, i.e. transmission or reception using multiple antennas using two or more spaced independent antennas at the transmitting station
Techniques are described for metadata processing that can be used to encode an arbitrary number of security policies for code running on a processor. Metadata may be added to every word in the system and a metadata processing unit may be used that works in parallel with data flow to enforce an arbitrary set of policies. In one aspect, the metadata may be characterized as unbounded and software programmable to be applicable to a wide range of metadata processing policies. Techniques and policies have a wide range of uses including, for example, safety, security, and synchronization. Additionally, described are aspects and techniques in connection with metadata processing in an embodiment based on the RISC-V architecture.
G06F 21/52 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure
G06F 9/30 - Arrangements for executing machine instructions, e.g. instruction decode
G06F 9/38 - Concurrent instruction execution, e.g. pipeline or look ahead
G06F 12/0875 - Addressing of a memory level in which the access to the desired data or data block requires associative addressing means, e.g. caches with dedicated cache, e.g. instruction or stack
G06F 12/14 - Protection against unauthorised use of memory
G06F 21/62 - Protecting access to data via a platform, e.g. using keys or access control rules
G06F 15/78 - Architectures of general purpose stored program computers comprising a single central processing unit
89.
Optical switch controllable by vertical motion MEMS structure
MEMS-actuated optical switches can be implemented on photonic chips. These switches are compact, essentially planar, simple to implement and include only one moving MEMS component per switch. The switches exhibit low optical loss, require low power to operate, and are simple to control and easy to integrate with other optical devices. Each switch has two optical waveguides that are optically coupled in an ON switch state and not coupled in an OFF switch state. An end or a medial section of one of the two waveguides may translate between the ON and OFF states to affect the coupling. Alternatively, a coupling frustrator may translate between the ON and OFF states to affect the coupling.
A hardware system is configured for, and a method of, generating detail-rich gradient-based disparity maps in real-time using an automated gradient-based disparity map classification process that is scalable, can be used under different environment conditions with little to no restrictions, and whose level of precision can be adjusted in a scalable manner. Highly accurate cross-spectral stereo matching methods may be used for search and rescue operations and work at day time and night time using current and past visual and full infrared imaging to generate, classify, and identify scenes in real-time with minimum constraints. Such system and methods may be used to improve operations of existing search and rescue equipment.
A system and method of processing instructions may comprise an application processing domain (APD) and a metadata processing domain (MTD). The APD may comprise an application processor executing instructions and providing related information to the MTD. The MTD may comprise a tag processing unit (TPU) having a cache of policy-based rules enforced by the MTD. The TPU may determine, based on policies being enforced and metadata tags and operands associated with the instructions, that the instructions are allowed to execute (i.e., are valid). The TPU may write, if the instructions are valid, the metadata tags to a queue. The queue may (i) receive operation output information from the application processing domain, (ii) receive, from the TPU, the metadata tags, (iii) output, responsive to receiving the metadata tags, resulting information indicative of the operation output information and the metadata tags; and (iv) permit the resulting information to be written to memory.
G06F 9/38 - Concurrent instruction execution, e.g. pipeline or look ahead
G06F 21/52 - Monitoring users, programs or devices to maintain the integrity of platforms, e.g. of processors, firmware or operating systems during program execution, e.g. stack integrity, buffer overflow or preventing unwanted data erasure
G06F 12/14 - Protection against unauthorised use of memory
G06F 21/62 - Protecting access to data via a platform, e.g. using keys or access control rules
G06F 21/71 - Protecting specific internal or peripheral components, in which the protection of a component leads to protection of the entire computer to assure secure computing or processing of information
G06F 9/30 - Arrangements for executing machine instructions, e.g. instruction decode
G06F 12/1009 - Address translation using page tables, e.g. page table structures
G06F 21/57 - Certifying or maintaining trusted computer platforms, e.g. secure boots or power-downs, version controls, system software checks, secure updates or assessing vulnerabilities
G06F 12/0875 - Addressing of a memory level in which the access to the desired data or data block requires associative addressing means, e.g. caches with dedicated cache, e.g. instruction or stack
Systems and methods for cleansing blood are disclosed herein. The methods include acoustically separating target particles from elements of whole blood. The whole blood and capture particles are flowed through a microfluidic separation channel formed in a thermoplastic. At least one bulk acoustic transducer is attached to the microfluidic separation channel. A standing acoustic wave, imparted on the channel and its contents by the bulk acoustic transducer, drives the formed elements of the blood and target particles to specific aggregation axes.
According to various aspects and embodiments, a growth adaptive expandable stent is provided. The expandable stent includes a stent structure having a cylindrical shape that is self-expanding in a radial direction and includes a plurality of cylindrical rings disposed along a longitudinal axis of the stent structure. The stent structure is configured to exert a continuous outward radial force over time when implanted such that a diameter of the stent structure expands from a first value to a second value that is at least about 1.5 times the first value.
A61F 2/89 - Stents in a form characterised by wire-like elementsStents in a form characterised by a net-like or mesh-like structure the wire-like elements comprising two or more adjacent rings flexibly connected by separate members
A LiDAR system emits single mode light from a photonic integrated circuit (PIC) and is capable of receiving a different mode light, or multiple modes of light, into the PIC. Objects in the LiDAR's field of view may reflect light with a mode different from the mode of the light that illuminated the objects. Thus, in some embodiments, a single-mode optical waveguide, a single-mode-multi-mode optical junction, a multi-mode optical waveguide and an array of optical emitters on the PIC are configured to emit into free space light of a single mode from each optical emitter of the array of optical emitters. The multi-mode optical waveguide and the array of optical emitters are configured to receive from the free space light of a mode different from the single mode, or multiple modes, and to couple the light of the different mode or multiple modes into the multi-mode optical waveguide.
The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.
A LiDAR system includes a light source and an arrayed micro-optic configured to receive light from the light source so as to produce and project a two-dimensional array of light spots on a scene. The LiDAR system also includes receiver optics having an array of optical detection sites configured so as to be suitable for establishing a one-to-one correspondence between light spots in the two-dimensional array and optical detection sites in the receiver optics. The LiDAR system further includes a birefringent prism and a lens. The LiDAR system may also include a mask placed in the light path between the birefringent prism and the receiver optics. Alternatively, the LiDAR system may include a controller programmed to activate or deactivate each optical detection site.
A well reader and actuator (100) for a well array (50) has an array of detectors, each detector for detecting a fluorescence signal from fluorophores in a respective well of the well array and an excitation subsystem for exciting the fluorophores in the wells of the well array. Embodiments of this invention can be used to carry out two important functions in a highly parallel manner: by addressing individual wells in a 32, 96, 384 etc. well array, where each well contains a potential chemical or biological reaction. The two functions are: 1) through thermal, optical or other means and combinations thereof the rate of a chemical or biological reaction is controlled or gated (e.g. colder wells inhibit a reaction, or an enzymatic reaction requires blue light to proceed); and 2) through use of fluorescent species that are sensitive to the target reaction—or reactions—an optical readout of fluorescent intensity and/or lifetime is tracked to monitor the evolution of the reaction.
A method for separating particles in a biofluid includes pretreating the biofluid by introducing an additive, flowing the pretreated biofluid through a microfluidic separation channel, and applying acoustic energy to the microfluidic separation channel. A system for microfluidic separation, capable of separating target particles from non-target particles in a biofluid includes at least one microfluidic separation channel, a source of biofluid, a source of additive, and at least one acoustic transducer coupled to the microfluidic separation channel. A kit for microfluidic particle separation includes a microfluidic separation channel connected to an acoustic transducer, a source of an additive, and instructions for use.
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SE (USA)
THE CHARLES STARK DRAPER LABORATORY, INC. (USA)
Inventor
Glaven, Sarah
Onderko, Elizabeth
Maygar, Andrew
Yates, Matthew
Abstract
Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE NAVY (USA)
THE CHARLES STARK DRAPER LABORATORY, INC. (USA)
Inventor
Glaven, Sarah
Onderko, Elizabeth
Maygar, Andrew
Yates, Matthew
Abstract
Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.
