The present invention belongs to the technical field of plant biology. Provided is a method for creating and propagating a recessive genic male sterile line of a plant. According to the method, by means of editing a recessive genic male sterile gene and simultaneously performing complementation in a plant, a sterile line and a maintainer line are rapidly created. At the same time, by means of a color marker in the seed and the plant, rapid screening and propagation of the sterile line and the maintainer line are realized. Furthermore, a pollen-lethal element is linked, improving the propagation efficiency of the sterile line. Thus, a method for rapidly creating and efficiently propagating a recessive genic male sterile line of a plant is established, effectively improving the heterosis utilization efficiency of crops.
The present invention relates to a Cas effector protein, a fusion protein containing the protein, and a nucleic acid molecule encoding same. The present invention also relates to a complex and a composition for nucleic acid editing (e.g., gene or genome editing), which complex and composition contain the protein or fusion protein, or the nucleic acid molecule encoding same. The present invention further relates to a method for nucleic acid editing (e.g., gene or genome editing).
The present invention relates to the field of nucleic acid editing, and in particular to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, provided are Cas effector proteins, fusion proteins comprising such proteins, and nucleic acid molecules encoding same. Also provided are a complex and a composition for nucleic acid editing (e.g., gene or genome editing), comprising a Cas effector protein or a fusion protein, or a nucleic acid molecule encoding same. Also provided is a method for nucleic acid editing (e.g., gene or genome editing), which method uses a material comprising the Cas effector protein or the fusion protein.
A method for modifying a gliadin and an application thereof are provided. The method includes the following steps: dissolving the gliadin in a solvent to obtain gliadin solution; and adding a proline endoprotease to the gliadin solution for a reaction, and adjusting the pH value of solution to neutral according to a gradient, to obtain a modified gliadin. The modified gliadin obtained by the present invention may be uniformly dispersed in water, and may still maintain a uniform dispersion state in the water after high-temperature thermal sterilization and long-term storage, or even in the presence of sodium chloride, it has good thermal stability, salt resistance, and storage stability.
The present invention discloses a method for preparing ice cream with good storage and transportation stability. The raw materials of the ice cream include small-granule starch. The amount of small-granule starch added is 1% to 6%. By adding small-granule starch to the ice cream, the structure of the ice cream is improved, resulting in ice cream with good storage and transportation stability. It can maintain ideal hardness at high storage and transportation temperatures while retaining good shape, texture, and sensory qualities. This enhances the ice cream product's resistance to temperature fluctuations during cold chain storage and transportation, making it suitable for storage and sales temperatures higher than conventional storage temperatures (−18° C.), thus promoting energy saving and carbon reduction in the ice cream storage and transportation process.
A23G 9/22 - Details, component parts or accessories of apparatus insofar as not peculiar to a single one of the preceding groups
A23G 9/32 - Frozen sweets, e.g. ice confectionery, ice-creamMixtures therefor characterised by the composition
A23G 9/34 - Frozen sweets, e.g. ice confectionery, ice-creamMixtures therefor characterised by the composition characterised by carbohydrates used, e.g. polysaccharides
6.
GREEN EXTRACTION METHOD FOR CHROMOPLAST, RESULTING PRODUCT AND USE
Disclosed are a green extraction method for a chromoplast, a resulting product and a use. The green extraction method comprises: using plant tissues as a raw material, and adding an extraction solvent and homogenizing; filtering, and then collecting a filtrate; carrying out centrifugal separation, and discarding a supernatant to obtain a crude extract; and carrying out sucrose solution gradient separation, aspirating an enrichment layer, adding an extraction solution, carrying out centrifugal separation, and discarding a supernatant to obtain a pure chromoplast extract. The chromoplast extract obtained using the green extraction technique in the present invention has no significant difference in lipid solubility compared to carotenoids extracted using organic reagents, but has significantly better water solubility than the carotenoids extracted using the organic reagents, and also has a significant advantage in rehydration rate.
The present invention discloses a ZmWAK-RLK protein related to gray leaf spot resistance, and an encoding gene and application thereof. The present invention sets forth a protein obtained from a maize inbred line Y32, named ZmWAK-RLK, and being the protein shown in sequence 1 of the sequence listing. A nucleic acid molecule for encoding the ZmWAK-RLK protein is likewise considered to be within the scope of protection of the present invention. The present invention likewise sets forth a method for preparing a transgenic plant, comprising the following step: introducing said nucleic acid molecule into a starting plant, thus obtaining a transgenic plant having enhanced resistance to gray leaf spot. The present invention additionally sets forth a plant breeding method, comprising the following step: increasing the content and/or activity of the ZmWAK-RLK protein in a plant of interest, thus increasing the gray leaf spot resistance of the plant of interest. The present invention has great value when applied to the breeding of maize resistant to gray leaf spot.
This invention relates to the field of genetic molecular breeding, specifically to a pig 50K liquid-phase chip based on multiple single nucleotide-polymorphism and its application. The probe design of the chip in this invention takes into account the distribution of captured SNP loci across the genome, the polymorphism of the loci, the quality of mSNP markers, and other issues, effectively avoiding problems such as uneven marker density and poor polymorphism. It also considers the quality of mSNP markers and the issue of linkage disequilibrium among mSNP markers. While adhering to the basic principles of liquid-phase chip design, genomic regions with moderate linkage disequilibrium between markers were selected, generating more SNP markers with high genotyping quality and moderate linkage disequilibrium within the probe region with the target loci. The mSNP liquid-phase chip of this invention expands the detectable number of SNPs to 1.5-2 times that of the target loci, addressing the issue of the relatively small number of high-quality mSNP markers in traditional liquid-phase chips without increasing costs.
This invention relates to the field of genetic molecular breeding, specifically to a pig 10K liquid-phase chip based on multiple single nucleotide-polymorphism and its application. The present invention, while adhering to the basic principles of liquid phase chip design, adds new markers to existing chips and optimizes probes using multiple single nucleotide-polymorphism technology. This results in the generation of more SNP markers with high genotyping quality and moderate linkage disequilibrium with target SNP loci within the probe region, thereby increasing the effective marker count of the chip. The mSNP liquid phase chip of the present invention increases the number of detectable SNPs to 1.5-2 times the number of target SNP markers, addressing the issue of existing chips that only contain target SNPs and cannot provide mSNP markers, without increasing the cost. By using genotype imputation technology, genotype data from the chip of the present invention is imputed into mainstream 50K chips for genomic selection, achieving molecular breeding accuracy comparable to or similar to that of the 50K chips, thereby reducing the cost of pig molecular breeding.
The present disclosure relates to Lactiplantibacillus plantarum EL6 for increasing the flavonoids content, and a use thereof, and belongs to the technical field of microbial feeds. The present disclosure is intended to solve the problem that there is no special lactobacillus agent for increasing the flavonoids content during silage fermentation in the prior art. The Lactiplantibacillus plantarum EL6 in the present disclosure is a Gram-positive bacterium, which allows glucose facultative fermentation and has advantages such as acid resistance and high growth rate. In addition, the Lactiplantibacillus plantarum EL6 has an ability to allow the biotransformation of flavonoids, and can significantly increase a content of flavonoids in Medicago silage and promote the release and transformation of bound flavonoids. The Lactiplantibacillus plantarum EL6 can improve a fermentation quality of silage, and has advantages such as low cost, safety, reliability, and easy utilization.
A23K 10/18 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
A23K 10/30 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms
The present disclosure relates to Lactiplantibacillus plantarum for degrading tannins and saponins, and a use thereof. The present disclosure is intended to solve the problem that the existing silage has a low utilization rate due to anti-nutritional factors such as tannins and saponins. The present disclosure provides Lactiplantibacillus plantarum P91 with an accession number of CGMCC No. 27567, which has acid resistance, a high growth rate, a strong carbon source-utilizing ability, and an excellent comprehensive acid-production ability, and can reduce a pH, well improve the palatability of silage, and reduce anti-nutritional factors in silage. Therefore, the present disclosure can effectively solve the problem that a silage raw material has a high content of anti-nutritional factors such as tannins and saponins, and overcome the adaptability problem of a lactobacillus to a raw material, thereby improving an activity of the lactobacillus and a quality of silage fermentation.
A23K 10/18 - Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
A23K 10/30 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms
A cell growth promoter and an application thereof are provided. The invention adopts Fe2+ and ovalbumin to form a Fe2+-ovalbumin (OVA) compound to promote ovalbumin cell endocytosis, the cell growth promoter can maintain cell growth in an environment free of amino acids, promote cell proliferation in an environment with amino acids; and, the Fe2+-OVA compound can replace expensive animal serum albumin in cell culture, the cell growth promoter has a significant effect on improving cell viability and is low cost.
Provided is a myogenic differentiation induction method for a porcine pluripotent stem cell. The method does not involve transgenesis and does not involve serum addition in the whole process. In addition, further provided is a culture meat (CM) preparation method based on said myogenic differentiation induction method.
Lactobacillus casei strain that rapidly metabolizes lactose is screened through high expression of the lac cluster gene, such that the problem of large differences in acid production in fermented milk beverage products is avoided.
A method and device for predicting the bull semen quality. The method comprises: on the basis of the variation of methylation level values, using known methylation spectrum data of methylation sites of a bull sperm to select 83 methylation sites; on the basis of an elastic net regression algorithm, using the selected methylation sites to construct an epigenetic clock; and using the epigenetic clock to predict the age of a bull to be detected. The age of a bull can be predicted on the basis of an epigenetic clock, and the bull semen quality can be predicted on the basis of an epigenetic acceleration. The present invention can be applied to bull variety breeding and bull breeding.
Disclosed are uses of a ZmST1 protein and a coding gene thereof in regulation and control of the stay-green, disease resistance and yield of a plant. A ZmST1 overexpression strain is constructed by overexpressing a ZmST1 protein in corn, and by analyzing the phenotypes of the leaf stay-green property and the disease resistance and the yield of the ZmST1 overexpression strain in the mature period, it is found that: compared with the wild type, the ZmST1 overexpression strain shows that the visible functional stay-green is prolonged, the field disease resistance is enhanced, the stem diameter of the cluster is increased, the grain is increased, and the yield is increased. It is indicated that the ZmST1 protein or the coding gene thereof can regulate and control the stay-green and disease resistance of the leaves in the mature period of the corn by participating in transferring of products of photosynthesis in chloroplasts of the corn, delay the aging process of the leaves, prolong the stay-green functional period of the leaves of the plant, and increase the yield of the corn. The present invention has important significance for cultivating high-yield corn.
A multiple single-nucleotide polymorphism (mSNP)-based 10K liquid phase chip for a pig, and a use thereof. Under the condition that the basic principle of liquid phase chip design is maintained, a new marker is added to an existing chip, and an mSNP technology is used to optimize a probe, so as to generate more SNP markers having high typing quality and medium linkage disequilibrium with a target site SNP in a probe area, thereby increasing the number of effective markers of the chip. According to the mSNP-based liquid phase chip, the number of detectable SNPs can be increased to be 1.5-2 times of the number of target site SNP markers, thereby solving the problem of existing chips only containing the target site SNP and being unable to provide an mSNP marker, without increasing the cost. By means of a genotype imputation technology, the genotype data of the chip is filled into a mainstream 50K chip for genome selection, thereby obtaining molecular seed selection accuracy identical or similar to that of the 50K chip, and reducing the molecular breeding cost of the pig.
Provided are a method, system and device for classifying feeding intensity of fish school, relating to the field of aquaculture. The method includes: extracting features of an audio clip to be detected to determine a Mel spectrum-based fish school feeding depth speech spectrum feature vector, a CQT-based fish school feeding depth speech spectrum feature vector, and an STFT-based fish school feeding depth speech spectrum feature vector; fusing the Mel spectrum-based fish school feeding depth speech spectrum feature vector, the CQT-based fish school feeding depth speech spectrum feature vector and the STFT-based fish school feeding depth speech spectrum feature vector to generate a fused feature spectrogram; and inputting the fused feature spectrogram into a deep convolutional neural network model constructed by historical audio clips corresponding to different types of feeding intensities to determine a feeding intensity type corresponding to the audio clip to be detected.
A01K 61/95 - Sorting, grading, counting or marking live aquatic animals, e.g. sex determination specially adapted for fish
G10L 25/18 - Speech or voice analysis techniques not restricted to a single one of groups characterised by the type of extracted parameters the extracted parameters being spectral information of each sub-band
G10L 25/21 - Speech or voice analysis techniques not restricted to a single one of groups characterised by the type of extracted parameters the extracted parameters being power information
G10L 25/30 - Speech or voice analysis techniques not restricted to a single one of groups characterised by the analysis technique using neural networks
G10L 25/51 - Speech or voice analysis techniques not restricted to a single one of groups specially adapted for particular use for comparison or discrimination
19.
PORCINE PLURIPOTENT STEM CELL CULTURE MEDIUM AND USE THEREOF
The present invention relates to a medium for porcine pluripotent stem cells and use thereof, specifically provides a medium comprising: a first component that is IWR-1-endo; a second component that is selected from WH-4-023 or A419259; and a third component that is selected from fibroblast growth factors. The medium is easy to support the establishment of stable porcine pluripotent stem cell lines, especially the porcine E8-10 pre-gastrulation epiblast stem cell lines (known as pgEpiSCs).
Provided is a CRISPR/Cas9-based multiplex gene editing method. In the method, a multiplex gene editing plasmid M-PX459 is used, and the multiplex gene editing plasmid M-PX459 is formed, on the basis of a plasmid PX459, by replacing a hU6 promoter in the plasmid PX459 with multiple hCtRNAs having the nucleotide sequence of SEQ ID NO. 1. By replacing the original hU6 promoter with hCtRNAs, M-PX459 can increase the number of target sequences within a limited plasmid length range, so as to achieve the aim of multiplex gene editing; and compared with the knockout of a single gene by the PX459 plasmid, the method of the present invention not only has the ability to realize multiplex gene editing, but also has the same editing efficiency.
A method for creating a seawater desalination reverse osmosis membrane that excels in both water flux and boron removal. The method utilizes the abundant and reactive amino groups of carbon nitride in an interfacial polymerization reaction to enhance the membrane's structure. The unique pore and interlayer structure of carbon nitride is employed to modify the membrane's hydrophilicity, roughness, and interlayer structure, thereby boosting its water flux and boron removal capabilities. Additionally, the carbon nitride solution demonstrates exceptional dispersion properties. Its hydrophilic amino groups react with the organic phase monomer trimesoyl chloride during polymerization, ensuring an even distribution in the polyamide layer without any agglomeration. The evenly dispersed m-phenylenediamine and carbon nitride solution, along with sodium hydroxide in the aqueous phase, quicken the acylation reaction rate. This not only ensures more uniform participation of carbon nitride in the reaction but also further enhances the membrane's water flux and boron removal efficiency.
B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
C08G 69/32 - Polyamides derived from amino carboxylic acids or from polyamines and polycarboxylic acids derived from polyamines and polycarboxylic acids from aromatic diamines and aromatic dicarboxylic acids with both amino and carboxylic groups aromatically bound
Pig embryo-derived pluripotent stem cells, having the characteristics and pluripotency of pig pre-gastrulation metaplasia epiblast cells. The solution is capable of stable passage, and is able to withstand multiple instances of consecutive gene editing and produce cloned pigs. The pig embryonic pluripotent stem cells create a new path for biological research, animal husbandry, and regenerative biomedicine.
A QTL gene of maize for tolerance to salt stress and drought stress, having a nucleotide sequence as shown in SEQ ID No: 1. An overexpression material of the gene enhances the salt resistance and drought resistance of maize, but a mutant material weakens the salt resistance and drought resistance of maize. The QTL of maize for salt resistance and drought resistance can be used for regulating the salt resistance and drought resistance of maize and breeding of salt- and drought-resistant maize varieties.
C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
A01H 6/46 - Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
24.
MULTIPLE SINGLE NUCLEOTIDE POLYMORPHISM-BASED PORCINE 50K LIQUID CHIP, AND USE THEREOF
A multiple single nucleotide polymorphism (mSNP)-based porcine 50K liquid chip, and a use thereof. The design of a probe of the chip takes the problems such as the distribution of captured SNP loci in whole genomes, locus polymorphism, and the quality of mSNP markers into account, effectively avoiding the problems of non-uniform marker density and poor polymorphism. The problems of the quality of mSNP markers and linkage disequilibrium between mSNP markers are also taken into account, and on the basis of maintaining the basic principle of liquid chip design, a genome region having medium linkage disequilibrium between markers is selected to generate more SNP markers having high typing quality and medium linkage disequilibrium with target loci in a probe region. The mSNP liquid chip increases the number of detectable SNPs to be 1.5-2 times or more of the number of target loci, solving the problem of a conventional liquid chip having few high-quality mSNP markers, without increasing cost.
A rice nitrogen of panicle fertilizer accurate recommendation method based on crop model and remote sensing coupling includes the following steps: S1. constructing a basic database; S2. agricultural parameter inversion modeling based on the remote sensing vegetation index; S3. rice nitrogen nutrition diagnosis based on the nitrogen nutrition index NNI; and S4. accurately recommending panicle fertilizer nitrogen based on a crop growth model and remote sensing nitrogen nutrition diagnosis. According to the method, the rice growth trend and nitrogen nutrition estimation in the key growth period is achieved through a remote sensing inversion technology, a multi-source remote sensing inversion spatial dataset based on the rice growth trend is taken as a combination point coupling crop growth model to simulate the rice yield in real time, the rice nitrogen nutrition condition and the nitrogen demand are further combined, and the plots-level nitrogen of panicle fertilizer accurate recommendation is finally achieved.
Provided are a fusion protein and use thereof in gene editing. Specifically, disclosed is a fusion protein for improving gene editing efficiency, and the fusion protein is a protein having an amino acid sequence of SEQ ID No. 9, SEQ ID No. 11, or SEQ ID No. 13. Also provided are a gene editing system and a method for improving Cas12i3 protein-mediated gene editing efficiency. A coding sequence nucleic acid molecule combination of T5 exonuclease and a linker capable of significantly improving the Cas enzyme editing efficiency is acquired by means of screening. Compared with an unmodified Cas12i3 protein, the fusion protein acquired by the combination exhibits significantly improved editing efficiency. Further flow cytometry analysis results show that by means of modification with the C-linker 3 combination, the editing efficiency of the Cas enzyme is further significantly improved. The provided gene editing system features high editing efficiency and possesses high application values and broad prospects.
Provided is the use of a ZCN8 protein or a ZCN8 gene in the regulation and control of drought resistance in a plant. Also provided is a method for breeding a drought-resistant plant, comprising increasing the content of the ZCN8 protein in a plant so as to improve the drought resistance thereof. Further provided is a method for preparing a transgenic plant with enhanced drought resistance, comprising introducing the ZCN8 gene or a ZCN8 gene-related biological material into a receptor plant to obtain a transgenic plant of which the drought resistance is higher than that of the receptor plant. The ZCN8 protein is a protein as shown in sequence 1 in the sequence listing. The ZCN8 gene is a gene encoding the ZCN8 protein. The drought resistance is reflected in the fact that in the environment of drought stress, the ASI of a transgenic plant is lower than that of a starting plant. The drought resistance is reflected in the fact that in the environment of drought stress, the yield of a transgenic plant is higher than that of a starting plant. Also provided is a transgenic plant comprising the ZCN8 protein or the ZCN8 gene.
Provided herein are KRN2 gene controlling kernel row number in plant, molecular markers closely linked to KRN2 and their application in molecular breeding.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
29.
NEW USE OF WNT2 FOR INCREASING IN VITRO FERTILIZATION EMBRYO DEVELOPMENT EFFICIENCY AND QUALITY
Provided is a new use of WNT2 for increasing in vitro fertilization embryo development efficiency and quality, wherein in vitro fertilization embryo development efficiency and quality are increased by enhancing WNT signaling pathway activity in in vitro fertilization embryos. Specifically, obtained fertilized eggs are transferred into a culture medium supplemented with an appropriate concentration of WNT2 for in vitro culturing, so as to effectively increase the IVF blastocyst rate, improve the development potential, and increase the implantation rate and the birth rate after embryo transplantation. Low in vitro embryo production efficiency is a key factor restricting the development of livestock in vitro embryo production and even human assisted reproductive technology. WNT2 is an endogenous natural protein factor secreted by mammalian fallopian tubes, the action pathway and the target point are clear, and the safety of WNT2 in terms of IVF application is ensured. Therefore, an effective, safe and feasible strategy is provided for increasing livestock and human in vitro embryo production efficiency and quality.
A soil-climate intelligent type determination method for rice target yield and nitrogen fertilizer amount includes steps: construct a basic database; obtain historical weather data from the region where the rice planting area is to be determined over the past few years, and obtain real-time GFS weather data of the region in a predicted year; obtain 100 m×100 m grid sampling point soil data and a rice field vector layer of rice planting area to be determined; obtain at least three years of nitrogen fertilizer gradient test data of the rice planting area to be determined, rice variety information and management data of each farmer in each year; obtain accurate weather data of the prediction year; prepare soil data of 100 m×100 m plot-scale; localize genetic parameters of the rice growth model DSSAT; and run the localized DSSAT model to predict the plot-scale target yield and recommend the nitrogen application amount.
The present invention relates to the technical field of agricultural chemistry, in particular to butenolide compounds containing a thiazolidinone structure as well as a preparation method therefor and the use thereof The butenolide compounds containing a thiazolidinone structure are characterized by having the following structural general formula (I). The compounds disclosed by the present invention have excellent fungicidal activity on various pathogens in agriculture or other fields, and can favorably protect important crops and domestic animals in agriculture and horticulture industry, and the environment that the human beings rely on from invasion of pathogens.
C07D 417/06 - Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
A preparation method for a convenient and instant dumpling with a long normal-temperature shelf life includes the following steps: preparation of dough→pressing of dough wrapper→preparation of dumpling wrapper→preparation of stuffing→kneading of dumpling→pre-cooking→canning→sterilization and finished product. By improvement processes in the angle of dough wrapper, stuffing, water migration, dough wrapper adhesion, and taste preservation, the product performance is improved, so that the product may be stored at the normal temperature for at least 12 months and the taste quality may still maintain the same level as fresh dumplings.
A21D 2/02 - Treatment of flour or dough by adding materials thereto before or during baking by adding inorganic substances
A21D 8/02 - Methods for preparing doughTreating dough prior to baking
A23L 3/10 - Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating materials in packages which are not progressively transported through the apparatus
A23L 5/10 - General methods of cooking foods, e.g. by roasting or frying
An in-site composting system for community kitchen waste is provided, which includes: a dump unit, configured to receive a kitchen waste; a power energy conversion unit, configured to convert a part of energy generated by a fitness equipment or a rocking handle into electrical energy, and output a remaining part of the generated energy directly as power, a biological conversion unit, configured to receive the kitchen waste fed by the dump unit and perform a composting and fermentation processing on the kitchen waste; an energy storage unit, configured to store the electrical energy generated by the power energy conversion unit and the solar panel; and a material outputting and returning unit, configured to be connected to the biological conversion unit, wherein the material outputting and returning unit is configured to output and return a material decomposed by the biological conversion unit.
Use of a C-JUN N-terminal kinase inhibitor SU3327 in the preparation of a drug for reducing cytotoxicity and renal toxicity of polymyxin. The polymyxin is preferably polymyxin E. SU3327 combined with polymyxin E can significantly inhibit the cytotoxicity and renal toxicity effects of polymyxin E.
The present invention relates to a method for identifying a precursor cell of a hair follicle placode in a porcine embryonic period, and the use thereof. BMP7 and TGFβ2 are used as marker genes of a precursor cell of a hair follicle placode in a porcine embryonic period. The identification method comprises: performing immunofluorescent staining on epidermal cells of a porcine embryo. A cell in which BMP7 and TGFβ2 can be simultaneously detected is the precursor cell of the hair follicle placode. The porcine embryo is an embryo on or before day 37 of the porcine embryonic period. In the present invention, the key marker genes BMP7 and TGFβ2 for identifying a precursor cell of a hair follicle placode in a porcine embryonic period are provided, and the expression and detection of the key marker genes can be performed on skin cells in an early porcine embryonic period by means of using a cellular immunofluorescence detection technique, so as to screen the precursor cell of the hair follicle placode.
A method for making an anthocyanin turn blue, comprising: mixing an anthocyanin with a protein to obtain a mixed solution; and standing the mixed solution so that the anthocyanin turns blue. The method does not involve ultra-high pressure treatment; the concentration of the anthocyanin in the mixed solution is 0.05-0.2 mM; the anthocyanin is selected from cyanidin-3-glucoside, the protein is selected from human serum protein or lysozyme, and in the mixed solution, the concentration of the human serum protein is 0.1-2 mM, and the concentration of the lysozyme is 0.8-1.5 mM; or, the anthocyanin is selected from malvidin-3-glucoside, the protein is selected from human serum protein or bovine serum protein, and in the mixed solution, the concentration of the human serum protein or bovine serum protein is 0.1-0.5 mM; the pH value of the mixed solution is 5.0-9.0.
A vibration self-generating battery of a water quality low-power sensor applicable to multiple scenarios, belonging to the technical field of self-generating energy supply. The battery includes a positive electrode magnetic cap, a battery outer cylinder, a negative electrode magnetic cap, a circuit module, a supporting component, an electromagnetic induction generating unit, and a friction nano generating unit. The positive electrode magnetic cap is arranged on the upper side of the battery outer cylinder, and the negative electrode magnetic cap is arranged at the lower side of the battery outer cylinder. The friction nano generating unit is arranged close to an inner wall of the battery outer cylinder. The electromagnetic induction generating unit is arranged at the center of the battery, and the supporting component and the circuit module are arranged below the electromagnetic induction generating unit in turn.
H02J 7/00 - Circuit arrangements for charging or depolarising batteries or for supplying loads from batteries
H02K 35/02 - Generators with reciprocating, oscillating or vibrating coil system, magnet, armature or other part of the magnetic circuit with moving magnets and stationary coil systems
38.
SENSOR FAULT DIAGNOSIS METHOD, APPARATUS, ELECTRONIC DEVICE AND STORAGE MEDIUM
The disclosure provides a sensor fault diagnosis method, an apparatus, an electronic device and a storage medium. The method comprises: determining target concentration of electrolyte in a sensor at time t; determining residual concentration based on initial concentration and the target concentration of the electrolyte; acquiring an impedance value and determining a concentration measurement value based on the impedance value and correlation information between concentration and the impedance; determining fault information of the sensor based on the residual concentration and the concentration measurement value. The concentration of the electrolyte is estimated by using an impedance calibrating method in combination with theoretical calculation, and the sensor fault diagnosis is realized by establishing a difference comparison interval between the concentration measurement value and a theoretical calculation value of the electrolyte, thereby saving the cost of fault diagnosis and improving the accuracy of fault diagnosis.
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
The present invention relates to the field of nucleic acid editing, in particular to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). In particular, the present invention relates to a Cas effector protein, a fusion protein comprising said protein, and a nucleic acid molecule encoding same. The present invention also relates to a complex and composition used for nucleic acid editing (e.g. gene or genome editing), which comprise the protein or fusion protein of the present invention, or the nucleic acid molecule encoding same. The present invention also relates to a method for nucleic acid editing (e.g. gene or genome editing), which uses the protein or fusion protein of the present invention.
The present invention relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). In particular, the present invention relates to a Type I-A CRISPR-Cas effector protein and system, a fusion protein comprising the protein, and nucleic acid molecules encoding same. The present invention also relates to a complex and composition for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, and genomic structural variation), comprising the protein or fusion protein in the present invention, or nucleic acid molecules encoding same. The present invention further relates to a method for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single base editing, and genomic structural variation), using the protein or fusion protein comprised in the present invention.
The present disclosure provides an early warning method, apparatus, and system for a nitrogen concentration in industrialized aquaponic circulating water. The early warning method includes: obtaining video data of a sensitive fish farming container, where the video data includes a monitored movement behavior of a sensitive fish in the sensitive fish farming container, and the sensitive fish farming container is in communication with a farming pond of an aquaponic system; processing the video data using an object detection model and obtaining a behavior activity of the sensitive fish according to a processing result; processing the behavior activity using a neural network model to obtain a nitrogen concentration grade of the farming pond of the aquaponic system; and determining whether to send nitrogen concentration early warning information based on the nitrogen concentration grade.
INSTITUTE OF QUALITY STANDARD AND TESTING TECHNOLOGY FOR AGRO-PRODUCTS OF CHINESE ACADEMY OF AGRICUL (China)
China Agricultural University (China)
Inventor
Xu, Zhenzhen
Yang, Shini
Liao, Xiaojun
Abstract
The present disclosure discloses a preparation method and enrichment method of cyanidin-3-diglucoside-5-glucoside, which relates to the technical field of biochemical industry. Enzyme is used to catalyze the substrate to obtain cyanidin-3-diglucoside-5-glucoside. The temperature of the catalytic reaction is 20-40° C., and the reaction time is 10-30 h. This enzyme can convert various anthocyanins into cyanidin-3-diglucoside-5-glucoside, thereby increasing the content of target anthocyanin. The method is simple to operate, specifically improves the content of cyanidin-3-diglucoside-5-glucoside in the crude extract, and reduces the types of anthocyanins in the crude extract to a certain extent, thereby reducing difficulty of subsequent separation and purification. The use of organic reagents and equipment in the traditional enrichment and purification process is reduced, which is energy-saving and environmentally friendly, and the product obtained by catalysis is of good quality, high yield and high purity.
GUANGZHOU WANGLAOJI HEALTH INDUSTRY CO., LTD (China)
Inventor
Liao, Xiaojun
Li, Chuyuan
Yang, Huanzhi
Weng, Shaoquan
Zhao, Liang
Zheng, Rongbo
Wang, Yongtao
Zhao, Yang
Abstract
Provided are a method and system for simultaneously extracting polysaccharides of Rosa roxburghii Tratt, polyphenols of Rosa roxburghii Tratt, superoxide dismutase (SOD) of Rosa roxburghii Tratt, and Vc from Rosa roxburghii Tratt pomace. The method includes enzymatic hydrolysis treatment, grinding treatment, ultrasonic treatment, centrifugation treatment, ceramic membrane ultrafiltration treatment, macroporous adsorption resin treatment, elution treatment, evaporation and concentration treatment, spiral-wound membrane ultrafiltration treatment, and reverse osmosis treatment.
B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
Provided are a method and an apparatus for predicting a demanded net energy for maintenance, and an electronic device. The method comprises: acquiring a heart rate data of a target pig (110); and on the basis of the heart rate data and a pre-trained net energy demand prediction model, acquiring a demanded net energy for maintenance of the target pig, wherein the net energy demand prediction model is a neural network model acquired by a productivity parameter-based training (120). According to the method, a prediction based on the heart rate data of a pig is effectively introduced in the prediction process of the demanded net energy for maintenance of the pig. Moreover, the overall prediction process is simplified and optimized, so as to conveniently and quickly predict the demanded net energy for maintenance of the pig in real-time with improved reproducibility and applicability.
A61D 99/00 - Subject matter not provided for in other groups of this subclass
G16H 50/30 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indicesICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for individual health risk assessment
45.
WATER QUALITY EARLY WARNING SYSTEM AND METHOD FOR AQUAPONICS BASED ON ZEBRAFISH BEHAVIOR ANALYSIS
A water quality early warning system and method for aquaponics based on zebrafish behavior analysis is provided. The system includes a monitoring chamber, an infrared transceiver array, a signal driving and collecting board and a processor, where the infrared transceiver array includes infrared transceiver modules; each infrared transceiver module includes an infrared transmitting module and an infrared receiving module; the signal driving and collecting board is connected to the infrared transceiver array, and configured to drive the infrared transmitting module to transmit infrared light, collect induced currents generated by the infrared receiving module and generate a voltage matrix in M rows and N columns according to induced currents; the processor is connected to the signal driving and collecting board, and configured to calculate motion parameters of the target zebrafish according to the voltage matrix, and perform an early warning on a water quality of the target water body.
A panoramic-scanning-based method for measuring the total number of muscle tissue fibers. Panoramic scanning is performed on muscle tissue sections by means of a panoramic scanner, so as to obtain a digital panoramic section, and the effect of counting the total number of all muscle fibers in muscle tissue is achieved by means of performing segmentation, counting and summation on the obtained digital panoramic section. Compared with a conventional method which relies on an optical microscope, takes five to ten image fields-of-view to represent histological characteristics of muscle fibers of a biological individual, and further takes a product of an average muscle fiber density and the area of a muscle section as an estimated value of the total number of muscle fibers in the section, the present method can implement full-field-of-view counting of muscle tissue, has less room for error and produces more accurate results.
A primer group for simultaneous detection of 15 porcine pathogens through high-throughput targeted amplicon sequencing and use thereof are provided. The primer group for simultaneous detection of 15 porcine pathogens through high-throughput targeted amplicon sequencing includes: primers having nucleotide sequences set forth in SEQ ID NO: 1 to SEQ ID NO: 302. The nucleotide sequences of pathogenic target genes are set forth in SEQ ID NO: 303 to SEQ ID NO: 344. The primer group of the present invention is used for amplicon sequencing detection analysis method for 42 target genes of 15 porcine respiratory pathogens, has the technical advantages of rapidness, high efficiency, high targeting, strong specificity, and the like, and is suitable for rapid and accurate simultaneous diagnosis of multiple pathogens of diseased pigs in pig farms.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]
C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
A method for determining a total number of fibers in a muscle tissue based on panoramic scanning is described. A muscle tissue slide is panoramically scanned to obtain a panoramic digital slide using a panoramic scanner, and the panoramic digital slide obtained is segmented, counted and summed to realize the statistical effect on the total number of fibers in the muscle tissue; compared with dependence on an optical microscope, 5-10 fields of view of an image are represented as histological characteristics of the muscle fiber of an biological individual, and further compared with a method using a product of average muscle fiber density and muscle slide area as an estimated value of the total number of fibers of the slide, the present disclosure can realize full-field statistics of the muscle tissue, with a smaller error and a more accurate result.
The present invention provides use of an N-terminus kinase inhibitor SU3327 of C-JUN in preparing a drug for enhancing anti-bacterial-infection efficacy of polymyxin and use of a composition of the SU3327 and the polymyxin in preparing a drug with enhanced anti-bacterial-infection efficacy. The polymyxin is preferably polymyxin E. The present invention proves by means of a chessboard-method minimum inhibitory concentration test and an in-vitro bacterial growth curve that the SU3327 synergistically enhances the antibacterial activity of polymyxin, and meanwhile, proves by means of a mouse drug-resistant bacterial infection model experiment at the animal level that the SU3327 can effectively enhance the polymyxin and the effectiveness in vivo. The present invention provides new use of the SU3327 in enhancing the antibacterial activity of polymyxin antibiotics and can solve the technical problems of clinical drug resistance, low therapeutic index, and the like of polymyxin.
Disclosed are a method for detecting milk production properties of dairy cows using an APOM gene SNPs molecular marker and use. The present invention provides use of a substance for detecting genotypes of the following four SNP sites in the identification or auxiliary identification of milk production properties of dairy cows: 23:g.27644015C>G and/or 23:g.27643343G>T and/or 23:g.27642597A>G and/or 23:g.27640015G>C; the sites are related to 305-day milk production, milk fat yield, milk fat percentage, milk protein yield, or milk protein percentage, and can be used for cow breeding.
The First Hospital of Hebei Medical University (China)
Inventor
Fu, Xiangwei
Li, Jun
Zhou, Dan
Zhou, Guizhen
Hou, Yunpeng
Abstract
A method for improving the effect of the oocyte cryopreservation by reducing the mitochondrial temperature is provided. The invention uses a culture medium containing metformin for pretreatment before oocyte freezing; the pre-treated oocytes are then placed in a 20% ethylene glycol solution for balancing, and then the oocytes are placed in a vitrification solution for freezing and are submerged into liquid nitrogen. The method of this invention can effectively reduce the mitochondrial temperature of porcine oocytes, reduce the fluidity of cell membrane, and does not affect the embryonic development after parthenogenetic activation, it can restore the mitochondrial temperature of oocytes after thawing, improve the survival rate after thawing, which can effectively improve the utilization rate of frozen oocytes, it has wide application prospect.
The present disclosure relates to the technical field of growth environment control for plants, and provides a light regulation method, system, and apparatus for a growth environment of leafy vegetables. The method includes: obtaining a growth environment image and growth environment light parameters of leafy vegetables; inputting the growth environment image into an image segmentation model to obtain an initial pixel segmentation map; determining a leaf area of the leafy vegetables according to the initial pixel segmentation map by using a Class Activation Mapping (CAM)-K algorithm; inputting the leaf area and the growth environment light parameters of the leafy vegetables into a leafy vegetable growth index prediction model to obtain a growth index of the leafy vegetables; adjusting the growth environment light parameters according to the growth index of the leafy vegetables. This disclosure achieves automated and intelligent control of light conditions in the growth environment of leafy vegetables.
G06T 7/62 - Analysis of geometric attributes of area, perimeter, diameter or volume
G06T 7/90 - Determination of colour characteristics
G06V 10/26 - Segmentation of patterns in the image fieldCutting or merging of image elements to establish the pattern region, e.g. clustering-based techniquesDetection of occlusion
G06V 10/762 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using clustering, e.g. of similar faces in social networks
G06V 10/774 - Generating sets of training patternsBootstrap methods, e.g. bagging or boosting
G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
COMPOSITE SOLUTION FOR ENHANCING INDUCED DISEASE RESISTANCE OF LENTINAN (LNT) TO PLANT, PREPARATION METHOD OF COMPOSITE SOLUTION, AND METHOD FOR ENHANCING INDUCED DISEASE RESISTANCE OF LNT TO PLANT
A composite solution for enhancing induced disease resistance of lentinan (LNT) to a plant, a preparation method of the composite solution, and a method for enhancing induced disease resistance of LNT to a plant are provided. The composite solution for enhancing induced disease resistance of LNT to a plant includes: an LNT-containing solution and an SPc-containing solution, where SPc is a dendritic macromolecule functionalized by an amino functional group, and has a structural formula shown in formula I, where n=1 to 100. An LNT/SPc complex is produced in the composite solution. SPc spontaneously combines with LNT through hydrogen bonding, such that an agglomerate structure formed by LNT in an aqueous solution is broken and reduced to a nano-scale particle size, and a spherical particle is produced, which can significantly reduce a contact angle of the LNT aqueous solution, and promote the distribution and diffusion of LNT.
A01N 25/22 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
A01N 43/16 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atom with one hetero atom six-membered rings with oxygen as the ring hetero atom
54.
USE OF NOVEL LOCUS OF CALLIPYGE GENE IN SHEEP BREEDING
Disclosed in the present invention is the use of a differentially methylated region of callipyge gene in meat trait breeding. Disclosed in the present invention is a differentially methylated region from an imprinted region Dlk1-Mirg of sheep callipyge gene, the nucleotide sequence of the differentially methylated region being shown as SEQ ID NO: 1. The differentially methylated region and an associated gene Gtl2 thereof can be used for early-stage breeding of meat traits of sheep. Further disclosed in the present invention is that a differentially methylated region shown as SEQ ID NO: 2 of a Dlk1-Mirg region of mice and an associated gene Meg3 thereof can be used for regulating and controlling the body weight of mice and constructing a weight loss mouse model.
C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
55.
METHOD AND SYSTEM FOR SIMULTANEOUSLY EXTRACTING ROSA ROXBURGHII TRATT POLYSACCHARIDE, ROSA ROXBURGHII TRATT POLYPHENOL, ROSA ROXBURGHII TRATT SOD, AND VC FROM ROSA ROXBURGHII TRATT POMACE
GUANGZHOU WANGLAOJI HEALTH INDUSTRY CO., LTD (China)
Inventor
Liao, Xiaojun
Li, Chuyuan
Yang, Huanzhi
Weng, Shaoquan
Zhao, Liang
Zheng, Rongbo
Wang, Yongtao
Zhao, Yang
Abstract
The present invention provides a method and system for simultaneously extracting Rosa roxburghii Tratt polysaccharide, Rosa roxburghii Tratt polyphenol, Rosa roxburghii Tratt SOD, and Vc from Rosa roxburghii Tratt pomace. The method comprises: enzymolysis treatment, pulping treatment, ultrasonic treatment, centrifugal treatment, ceramic membrane ultrafiltration treatment, macroporous adsorption resin treatment, elution treatment, evaporation concentration treatment, spiral-wound membrane ultrafiltration treatment, and reverse osmosis treatment.
Disclosed in the present invention is a novel method for improving the stress resistance of a meat-wool dual-purpose sheep. Provided in the present invention is a method for breeding a merino sheep having improved stress resistance in childhood and having a meat-wool dual-purpose in adulthood. The method comprises the following steps: mating a merino sheep having coarse wool in childhood as a female parent with a merino sheep having fine wool as a male parent, an obtained lamb offspring being a target merino sheep to be bred. Disclosed in the present invention is a mating method for improving the important environmental adaptation trait (stress resistance) and the important economic traits (weight and wool quality) of a sheep. Disclosed for the first time is mating the merino sheep having coarse wool in childhood as the female parent with the merino sheep having fine wool in childhood, so as to obtain the meat-wool dual-purpose sheep having fine wool, good wool quality, a heavy weight and great stress resistance.
Disclosed in the present invention are SNP molecular markers associated with pig body length, an amplification primer thereof, and use thereof. The molecular markers are located at the -416th and -288th bases (with the first base before ATG as -1) in a promoter region preceding the 5'UTR region of the pig HHEX gene, contain a C-T mutation and a G-A mutation, respectively, and are completely linked. It has been discovered for the first time that a pair of completely-linked SNP molecular markers in the pig HHEX gene are associated with the pig body length trait. Therefore, the markers can be used for marker-assisted selective breeding of pigs. That is, the present invention provides new use of a pair of completely-linked SNP molecular markers from the pig HHEX gene in marker-assisted breeding of pigs based on the pig body length trait and, by means of a simple and efficient method, realizes early screening based on the pig body length trait.
Provided in the present invention are an SNP molecular marker associated with intramuscular fat traits in pigs and the use thereof, and a method and kit for detecting the molecular marker. The SNP molecular marker is a base at position 12726 in the intron region of porcine DKK2 gene or a base at position 114874954 on porcine chromosome 8. Allele A is positively correlated with the trait of low intramuscular fat content, whereas allele C is positively correlated with the trait of high intramuscular fat content.
A predictive control system and regulatory method for a temperature of a livestock house are provided. The predictive control system includes a temperature and humidity sensor, a breeding environment temperature dynamic requirement module, an environmental controller and an environmental regulation implementation mechanism, where the environmental controller is connected to the breeding environment temperature dynamic requirement module and the temperature and humidity sensor; the environmental regulation implementation mechanism is connected to the environmental controller and is configured to perform corresponding environmental regulation according to a command from the environmental controller. The regulatory method controls a temperature of a breeding environment based on a livestock breeding environment temperature dynamic setting model, a livestock breeding environment temperature prediction system based on a GM (1,1) model, and a grey predictive fuzzy system based on the GM (1,1) model.
The present disclosure provides an oligopeptide FTLE isolated from chili pepper seeds, and application thereof in preventing or treating cancer, the oligopeptide is isolated from chili pepper seeds and has an anti-tumor effect, and in particular, the oligopeptide can effectively inhibit the growth and metabolism of HepG2 cells, and has a good application prospect.
Applications of a ZmROA1 protein or substance regulating the content or activity of the ZmROA1 protein are provided. The ZmROA1 protein can be specified as follows: A1), A2) or A3): A1, the amino acid sequence is the amino acid sequence shown in SEQ ID NO: 3; A2, the protein, which is obtained by replacing and/or deleting and/or adding the amino acid sequence shown in SEQ ID NO: 3 through amino acid residues, is more than 80% identity to A1) and relates to plant root angle; A3, the fusion protein which is obtained by linking N-end or/and C-end of A1) or A2) to protein tags. ZmROA1 protein and its related biological materials can be used to regulate plant density tolerance and/or root angle, and the cultivation of new density-tolerant maize varieties, which helps improve maize harvest index for high and stable yield.
The present application relates to a Pediococcus pentosaceus and application thereof. The Pediococcus pentosaceus is Pediococcus pentosaceus EL5 with deposit number of CGMCC No. 23923. The Pediococcus pentosaceus EL5 of an embodiment of the present application can be used for silage to improve the activity of lactic acid bacteria and the quality of silage fermentation.
A23K 30/18 - Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
A23K 10/12 - Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
The present disclosure provides a novel sugar- and phosphorus-containing fertilizer. In the present disclosure, based on masses of carbon and phosphorus elements, the novel sugar- and phosphorus-containing fertilizer has a carbon-phosphorus ratio of (1-15):1, and includes a low-carbon type with a carbon-phosphorus ratio of (1-4):1 and a high-carbon type with a carbon-phosphorus ratio of (4-15):1; and a carbon source is any one or more selected from the group consisting of glucose, sucrose, sugarcane molasses, and beet molasses. The novel sugar- and phosphorus-containing fertilizer is applied to the soil by drip irrigation or furrow dressing, and improves a phosphorus utilization efficiency of crops by regulating an activity of inherent phosphorus-solubilizing microorganisms in the soil. After applying the novel sugar- and phosphorus-containing fertilizer in a plurality of test sites, the soil has strengthened respiration, a significantly improved phosphatase activity, an obviously increased water-soluble phosphorus concentration, and remarkably enhanced crop production.
A method for improving the anti-oxidation and/or nutrient transport capabilities of placental tissues of female livestock, which comprises treating female livestock in a late stage of pregnancy using melatonin. Animal experiments prove that using melatonin for treating sows in a late stage of pregnancy can improve the concentration of melatonin in the peripheral blood and colostrum of the sows, and anti-oxidation and nutrition transport capabilities of placental tissues are also improved. The method is simple, effective, and highly feasible.
A deepwater oil and gas operation system based on geothermal energy supply, the system comprising a geothermal well system (1), an energy distribution system (2), a laser drilling system (3), an oil extraction and conveying system (4) and a multi-node wireless communication-ROV (5), wherein the geothermal well system and the oil extraction and conveying system are both connected to the energy distribution system by means of cables and pipelines, and the laser drilling system and the multi-node wireless communication-ROV are both connected to the energy distribution system by means of cables; the geothermal well system collects geothermal energy and converts some geothermal energy into electrical energy; the energy distribution system distributes the geothermal energy and the electrical energy; the laser drilling system supplies power to a laser drilling tool using the electrical energy converted from the geothermal energy; the oil extraction and conveying system uses the geothermal energy to perform heat preservation on an oil extraction pipeline; and the multi-node wireless communication-ROV controls an underwater unmanned inspection robot. The operation system realizes real-time information feedback control over the energy distribution system, the geothermal well system and the oil extraction and conveying system, thus effectively improving the intelligentization of the system.
E21B 43/01 - Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells specially adapted for obtaining from underwater installations
F24T 10/20 - Geothermal collectors using underground water as working fluidGeothermal collectors using working fluid injected directly into the ground, e.g. using injection wells and recovery wells
66.
SNP MOLECULAR MARKER COMBINATION FOR BEIJING BLACK PIG GENOTYPING, CHIP, AND PREPARATION METHOD THEREFOR AND USE THEREOF
The present invention relates to the technical fields of molecular biology, functional genomics, bioinformatics and genome breeding. Particularly disclosed are an SNP molecular marker combination for Beijing black pig genotyping, a chip, and a preparation method therefor and use thereof. The present invention relates to an SNP molecular marker combination for Beijing black pig genotyping, which is composed of 49,963 SNP molecular markers, wherein the nucleotide sequences of the SNP molecular markers are set forth in SEQ ID NOs: 1-49,963. The molecular marker combination can be applied to the preparation of a Beijing black pig molecular breeding chip, and can be further applied to the aspects of specifically identifying Beijing black pigs and other pig breeds (European commercial pigs and Chinese local pigs), target traits QTL, associated site and candidate gene identification, genetic relationship identification, family division, and the like.
Hydraulic design method, device and equipment for mobile drip irrigation system are provided. Different distances between drip-lines, a depth of a bottom boundary, a flux of infiltration boundary and soil hydraulic parameters are input into a simulation model, and output parameters of the model including a soil water content at each observation point and an amount of deep percolation loss under the different distances at the same irrigation depth are acquired. An optimal distance between drip-lines is obtained according to an installation cost function, the soil water content at each observation point and the amount of deep percolation loss, and lengths of drip-lines matched with the optimal distance are obtained. Structural parameters of the irrigation system and a designed irrigation depth are obtained to determine working parameters. The irrigation system with the optimal distance and the matched lengths of drip-lines is controlled to irrigate crops based on the working parameters.
A01G 25/02 - Watering arrangements located above the soil which make use of perforated pipe-lines or pipe-lines with dispensing fittings, e.g. for drip irrigation
A01G 25/09 - Watering arrangements making use of movable installations on wheels or the like
A cooked millet prolamin peptide for inhibiting α-amylase and α-glucosidase, an application thereof, and a screening method thereof are disclosed. The sequence of the peptide is selected from SEQ ID NOS: 1-6. The peptide provided in the present disclosure can effectively inhibit the activities of α-glucosidase and α-amylase simultaneously and is safe and non-toxic and has no side effects. Therefore, the peptide has a good potential and application prospect as a functional component in food, health products, and hypoglycemic drugs.
Provided are a method and an apparatus for predicting a demanded net energy for maintenance, and an electronic device. The method comprises: acquiring a heart rate data of a target pig (110); and on the basis of the heart rate data and a pre-trained net energy demand prediction model, acquiring a demanded net energy for maintenance of the target pig, wherein the net energy demand prediction model is a neural network model acquired by a productivity parameter-based training (120). According to the method, a prediction based on the heart rate data of a pig is effectively introduced in the prediction process of the demanded net energy for maintenance of the pig. Moreover, the overall prediction process is simplified and optimized, so as to conveniently and quickly predict the demanded net energy for maintenance of the pig in real-time with improved reproducibility and applicability.
Disclosed are a fertilizer composition containing a carbohydrate and phosphorus and a preparation method and use thereof, which belong to the technical field of agriculture. The fertilizer composition has a mass ratio of carbon to phosphorus of 1-15:1, and is divided into a low-carbon fertilizer composition (with a carbon-to-phosphorus ratio of 1-4:1) and a high-carbon fertilizer composition (with a carbon-to-phosphorus ratio of 4-15:1).
The present disclosure relates to the technical field of aquafarm monitoring, and in particular to a method, system and apparatus for intelligently monitoring an aquafarm with multi-dimensional panoramic perception. The method includes: analyzing preprocessed multi-dimensional panoramic perception information with time series analysis to obtain a linear fitting model; constructing a water quality forecasting-alarming model according to the linear fitting model; inputting fused data to a convolutional neural network (CNN) for training to obtain an electrical device fault diagnosis model; and monitoring an aquafarm with the water quality forecasting-alarming model and the electrical device fault diagnosis model.
A01K 63/04 - Arrangements for treating water specially adapted to receptacles for live fish
G06Q 10/04 - Forecasting or optimisation specially adapted for administrative or management purposes, e.g. linear programming or "cutting stock problem"
Provided is a method for reducing methane production in animal stomachs by adding melatonin to gastric juice of the animal or feeding the animal with melatonin. In the method of the present disclosure, 10−7 mol/L to 10−3 mol/L of melatonin can reduce the methane production from the in vitro gastric fermentation fluid, feeding melatonin of 8.0 to 35.0 mg/kg/day can reduce the methane production from the animal respiration from day 7 of feeding melatonin. The method of the present disclosure can reduce greenhouse gas emissions of animals, control environmental pollution and realize low-carbon farming.
A61K 31/4045 - Indole-alkylaminesAmides thereof, e.g. serotonin, melatonin
A61P 1/04 - Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
A61K 9/00 - Medicinal preparations characterised by special physical form
73.
Leuconostoc citreum and use thereof in precipitating starch emulsion
Leuconostoc citreum WSJ-57 deposited under an accession number of CGMCC No. 19201 in the China General Microbiological Culture Collection Center on Dec. 20, 2019.
The present disclosure belongs to the technical field of agricultural high-efficiency water-saving irrigation, and relates to a magnetization control apparatus for preventing clogging of a drip irrigation system irrigator and a control method thereof. The method includes: pumping irrigation water from the water source project through the first water pump, passing through the drip irrigation head control hub, and entering the pool; pumping the irrigation water through the second water pump, flowing through the first copper valve, and entering the magnetized pipe; the irrigation water after treated by the magnetizer returning to the pool through the flow rate monitor and the first valve in turn; when water quality of the irrigation water meets water quality requirements entering the capillary pipes, turning off the second water pump and stopping the cyclic magnetization treatment; the third water pump turning on; pumping the irrigation water after the cyclic magnetization treatment from the pool through the third water pump, and filtering through the mesh filter, entering the branch pipe and the capillary pipes through the second valve again; and the irrigation water flowing from the irrigators for irrigation. The present disclosure effectively solves the clogging problem of the drip irrigation system irrigator, has the advantages of cleanliness and high-efficiency; improves crop yield and quality, has a simple operation mode, and is convenient for users to operate.
C02F 1/00 - Treatment of water, waste water, or sewage
C02F 1/48 - Treatment of water, waste water, or sewage with magnetic or electric fields
A01G 25/02 - Watering arrangements located above the soil which make use of perforated pipe-lines or pipe-lines with dispensing fittings, e.g. for drip irrigation
75.
METHOD FOR TREATING RAW MATERIALS OF PLANT-DERIVED FOOD
The invention discloses a method for treating raw materials of plant-derived food, wherein the method comprises the following steps: mixing raw materials of plant-derived food with a solution containing photosensitizer to obtain a mixing liquid; carrying out light treatment of the mixing liquid; collecting raw materials in the mixing liquid subjected to light treatment, and performing drying treatment of the raw materials. The method can effectively realize sterilization effect, especially effectively reduce spore contamination, so as to ensure the safety of food and extend the shelf life; besides, the method can keep the original color, flavor, taste and nutrition contents of plant-derived food, and improve the edible value of plant-derived food.
Disclosed is a use of a ZmDi19 gene and a target gene ZmPR10 thereof in cultivating a gray leaf spot-resistant plant. Specifically disclosed are a use of the ZmDi19 and ZmPR10 genes and proteins encoded thereby in regulating and controlling plant disease resistance, and a method for cultivating a disease-resistant plant, particularly a gray leaf spot-resistant plant. Experiments show that in maize transgenic lines overexpressing the ZmDi19 and/or ZmPR10 genes, the disease index of transgenic plants was significantly reduced, and the disease resistance of the transgenic plants was significantly enhanced, indicating that the genes ZmDi19 and ZmPR10 have the function of gray leaf spot resistance and both positively regulate and control the gray leaf spot resistance of maize. The present invention provides good gene resources for regulating and controlling the gray leaf spot resistance of maize, has great application value for gray leaf spot-resistant breeding of maize, and is of great significance for ensuring high and stable yields of maize.
This invention provides a tofu with long shelf life and preparation method thereof, comprising soaking soybean to make soy milk, filtering to remove bean dregs, boiling, primary cooling, homogenizing, deaerating, UHT sterilization, secondary cooling, inline mixing soy milk with sterilized composite coagulant, aseptic filling, microwave heating, solidification, cooling for formation. According to this invention, a composite coagulant is prepared by combining basic salts with coagulant, then, the composite coagulant is inline mixed with soy milk, next, the mixture is intermittently heated by microwave to form tofu. The invention solves the problems existed in the preparation of tofu with long shelf life, including short temporary storage time, easy tube pasting and other issues after the coagulant is mixed with soy milk. This invention has the advantages of causing no corrosion on pipes, and taking less time in the heating and solidification process, etc. The long-shelf-life tofu produced by the invention has a shelf-life of more than 3 months at room temperature, while maintaining the original quality of the tofu, and has better taste and higher elasticity.
The present invention relates to a maize gene ZmRAVL1 and a functional site and use thereof. The present invention locates the functional site for controlling the leaf angle phenotype to 240 bp by fine-mapping, and the insertion and deletion of this region lead to different leaf angle phenotypes. The present invention demonstrates that an inbred line improved with an excellent natural variation from the teosinte can increase maize yield under dense planting and broaden the source of elite alleles available in plant breeding. The present invention demonstrates that a reduced ZmRAVL1 expression by a genetic engineering technique (RNAi) has an influence on plant architectures, such as a reduced leaf angle, and thus it provides excellent genetic resources for genetic engineering breeding. The present invention produces favorable alleles by adopting the gene-editing technology, and thus greatly shortens the selection process of elite alleles, which provides a new idea for obtaining elite alleles available in the breeding practice. The present invention can quickly and accurately improve or produce superior inbred lines with the aid of the molecular marker-assisted selection technology, which provides the possibility of wide application of elite alleles.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
79.
ENOATE COMPOUND CONTAINING AROMATIC RING, AND PREPARATION THEREFOR AND USE THEREOF
Disclosed are an enoate compound containing an aromatic ring, and a preparation therefor and the use thereof. The compound of the present invention is simple in terms of structure and easy to synthesize. Synthetic raw materials are derived from natural products, are pollution-free to the environment, and eco-friendly. The compound provided in the present invention has good repelling and killing effects on aphids, has good inhibitory activity on plant pathogenic fungi, can be used as an aphid control agent and a fungicide, and has good application prospects in green control of pests and plant diseases.
C07C 69/533 - Monocarboxylic acid esters having only one carbon-to-carbon double bond
C07C 69/86 - Esters of carboxylic acids having an esterified carboxyl group bound to a carbon atom of a six-membered aromatic ring of monocyclic hydroxy carboxylic acids, the hydroxy groups and the carboxyl groups of which are bound to carbon atoms of a six-membered aromatic ring with esterified hydroxyl groups
C07C 69/92 - Esters of carboxylic acids having an esterified carboxyl group bound to a carbon atom of a six-membered aromatic ring of monocyclic hydroxy carboxylic acids, the hydroxy groups and the carboxyl groups of which are bound to carbon atoms of a six-membered aromatic ring with etherified hydroxyl groups
C07C 69/587 - Monocarboxylic acid esters having at least two carbon-to-carbon double bonds
C07C 67/08 - Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
C07C 205/43 - Compounds containing nitro groups bound to a carbon skeleton the carbon skeleton being further substituted by esterified hydroxy groups having nitro groups or esterified hydroxy groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton to carbon atoms of the same non-condensed six-membered aromatic ring or to carbon atoms of six-membered aromatic rings being part of the same condensed ring system
C07D 209/08 - IndolesHydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
C07D 317/54 - Radicals substituted by oxygen atoms
A01N 43/38 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
A01N 43/30 - Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atom with two or more hetero atoms five-membered rings with two hetero atoms in positions 1,3 with two oxygen atoms in positions 1,3, condensed with a carbocyclic ring
A01N 37/40 - Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio-analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio-analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio-analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
The present disclosure provides a genomics-assisted prediction method for apple fruit quality traits and disease resistance and use thereof, and belongs to the technical field of plant genetics, genomics and breeding. The method is achieved by molecular markers. There are a total of 319 molecular markers, including 318 single-nucleotide polymorphism (SNP) markers and 1 InDel marker; and the molecular markers are associated with 16 traits of the Malus genus, including fruit ripening date, fruit shape, fruit cover color degree, fruit weight, soluble solid content, fruit juice pH value, malate content, chlorogenate content, procyanidin B2 content, flesh firmness at harvest, flesh crispness at harvest, flesh firmness retainability, flesh crispness retainability, fruit ring rot disease resistance, Glomerella leaf blotch resistance, and spur tree architecture. The molecular markers of the present disclosure can be used for apple germplasm resource evaluation and breeding, and can greatly improve apple breeding efficiency and shorten breeding cycle.
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
81.
PORCINE PLURIPOTENT STEM CELL CULTURE MEDIUM AND USE THEREOF
A porcine pluripotent stem cell culture medium and the use thereof. Specifically provided is a culture medium, which contains a first component, a second component and a third component, wherein the first component is IWR-1-endo; the second component is selected from WH-4-023 and A419259; and the third component is selected from fibroblast growth factors. The culture medium facilitates the support and establishment of a stable porcine pluripotent stem cell line, especially a porcine E8-10 pre-gastrulation epiblast (Epiblast stem cell line, also referred to as pgEpiSCs).
Pig embryo-derived pluripotent stem cells, having the characteristics and pluripotency of pig pre-intestinal metaplasia epiblast cells. The solution is capable of stable passage, and is able to withstand multiple instances of consecutive gene editing and produce cloned pigs. The pig embryonic pluripotent stem cells create a new path for biological research, animal husbandry, and regenerative biomedicine.
A detection device using a lateral flow strip for detection and a detection method thereof are provided, which relates to the technical field of a detection device using a lateral flow strip. An upper rotor, a middle rotor and a lower rotor are respectively provided with upper paddle(s), middle paddle(s) and lower paddle(s) along respective circumferential directions. Each upper paddle is provided with a test tube with openings at both ends thereof for placing the lateral flow strip. The middle paddle blocks a bottom one of the openings of the test tube. Each lower paddle is provided with a sample tube for placing sample solution. The bottom opening of the test tube is opposite to a top opening of the sample tube up and down.
A precise illumination regulation and control method for natural-mating cage-rearing breeding hens, comprising: performing combined regulation and control by using white light, red light, and blue light in different regions and different time periods in an egg laying period of the natural-mating cage-rearing laying hens, so as to reduce the occurrence of pecking addiction behaviors of the laying hens, reduce the death and culling rate caused by pecking addiction, reduce the content of stress hormones in blood, and improve the mating behavior frequency and a fertilization rate. The method improves the production efficiency and economical efficiency of the laying hens and the healthy breeding level.
An air pressure-machine vision based system for measuring a rheological property of a viscoelastic material includes a machine body, a lifting experiment table system, an air pressure generation control system, an image collection system, and a controlling and information processing system, where the lifting experiment table system, the air pressure generation control system, the image collection system and the controlling and information processing system are mounted on the machine body; the lifting experiment table system includes a lifting table stepping motor, an L-shaped lifting table and a lifting table motor driver, and the lifting table motor driver is connected to the lifting table stepping motor and configured to drive the lifting table stepping motor; and the lifting table stepping motor is connected to the L-shaped lifting table and configured to control lifting of the L-shaped lifting table.
A gene chip, a molecular probe combination, a kit and an application for analyzing sheep fat tails. Provided is a combination of 3000 SNP loci for analyzing sheep fat tail traits, the combination of loci being shown in Table 1, and biological products such as a molecular probe combination, a gene chip and a test kit prepared on the basis of the above combination of loci; by using the provided combination of loci and the related biological products, it is possible to perform genetic evaluation, breed screening and identification on individual sheep on a genetic level; in an early difficult-to-measure stage, the present invention can be used to perform individual selection on sheep fat tail traits, control the breeding process, and save breeding costs, and can also be used for sheep breed traceability, sheep pedigree reconstruction, germplasm resource protection and germplasm resource improvement.
Provided are a gene chip, molecular probe combination and kit for analyzing traits of sheep wool, and a use thereof, belonging to the technical field of biology. The molecular probe combination, gene chip, and kit are prepared on the basis of a combination of 2849 SNP sites, as discovered from research, capable of analyzing characteristics of the sheep wool, and are used for analyzing traits of the sheep wool. Detection of the traits, at the gene level, that are difficult to measure in the early stage not only can accelerate the breeding process and save breeding costs, but also can be used for the screening and identification of sheep varieties, reconstruction of sheep pedigree, protection of germplasm resources, and improvement of germplasm resources.
A gene chip, molecular probe combination, kit for sheep germplasm resource identification and pedigree reconstruction, and a use. Provided are 4213 SNP sites, and a gene chip, molecular probe combination, and kit prepared on the basis of the SNP sites. The physical positions of the sites are shown in Table 1. The present solution can be used for sheep germplasm resource identification and sheep pedigree reconstruction, and also can perform variety identification, variety tracing and sheep breeding control on sheep individuals and products thereof.
A gene chip, molecular probe combination and kit for analyzing sheep milk production performance, and a use, relating to the technical field of biology. A combination of 2030 SNP sites capable of analyzing sheep milk production performance characteristics is provided, and the physical position information thereof is determined on the basis of the comparison of sheep v4.0 genome sequences. By using the combination of 2030 SNP sites, and the molecular probe combination, gene chip and kit formed on the basis of the combination of sites, a genetic assessment on individual sheep can be performed, and individual selection on the milk production performance traits that are difficult to measure in the early stage can be made, thereby controlling the breeding process and saving the breeding cost.
The invention discloses the application of a β-N-acetylhexosaminidase (HaHex74) from Haloferula sp. in the synthesis of human milk oligosaccharides. The invention provides the use of HaHex74 protein or related biological materials thereof in any one of the following: synthesizing human milk oligosaccharides; synthesizing Lacto-N-triose II and/or Lacto-N-neotetraose; the HaHex74 protein having the amino acid sequence shown in SEQ ID No. 2 is derived from Haloferula sp. The β-N-acetylhexosaminidase HaHex74 disclosed by the invention possesses high-level expression, excellent hydrolysis properties and transglycosylation activity, which may make it potentially useful in the production of human milk oligosaccharides.
Provided are a parthenogenetic haploid induction gene DMP and an application thereof. The parthenogenetic haploid induction genes AtDMP8 and AtDMP9 are cloned from Arabidopsis thaliana. Experiments have shown that mutations of AtDMP8 and AtDMP9 can produce parthenogenetic haploid inducibility, to enable dicotyledonous crops to be induced to produce haploids via parthenogenetic means. The present invention was further verified in tomatoes, and it was also found in tomatoes that the mutation of SlDMP can produce parthenogenetic haploid inducibility. The invention lays an important foundation for broadening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenetic haploid production. Given the universality of the utilization of haploid breeding technology in the current breeding industry, the invention has very wide application space and market prospects.
Disclosed are a primer set for identifying a sex-linked dwarf gene and an application thereof. In the present invention, an upstream primer for detecting a dw gene is designed in a GHR gene deletion segment, an upstream primer for detecting a DW gene is designed upstream of the GHR deletion segment, and a downstream primer is uniformly designed downstream of the GHR deletion segment; amplification is simultaneously carried out on the interior of the deletion segment and the two ends of the deletion segment by means of multiple PCR reactions; in this way, ZDWZDWand ZDWW individuals can only detect an amplification product of a non-deleted segment, ZdwW and ZdwZdwindividuals can only detect a deleted segment, and a heterozygote ZDWZdw individual can simultaneously detect such two segments. The genotype of a chicken individual at a sex-linked dwarf gene locus can be rapidly and accurately identified by means of the present primer set.
A transaction harmony degree-based method and system for transaction matching between a power grid and building energy. The method includes: obtaining a transaction harmony degree of a historical transaction cycle; sending, by using a blockchain, the transaction harmony degree to a corresponding building energy user, and sending a maximum compensated electricity price and an expected compensated electricity price in peak-valley regulation to all building energy users; determining, by each building energy user, a transaction-based compensated electricity price in the peak-valley regulation based on the received transaction harmony degree and the received maximum compensated electricity price and expected compensated electricity price in the peak-valley regulation, and feeding back the transaction-based compensated electricity price in the peak-valley regulation to a power grid; and determining, by the power grid, a building energy user that successfully performs transaction matching with the power grid.
The present invention relates to the technical field of clustered regularly interspaced short palindromic repeats (CRISPRs). Provided are a method for nucleic acid editing (e.g., gene or genome editing, large fragment deletion, single-base editing, and genomic structural variation) and a composition, comprising the use of a Type I-C CRISPR-Cas3 system. The Type I-C CRISPR-Cas3 system can achieve accurate large fragment deletion of a genome, for example, knockout of any length of a single gene coding frame, knockout of gene regulatory elements such as long lncRNAs or enhancers, and single-base editing of genes or genomes and large structural variation in genomes.
A hydrophilic compound-embedded nano microcapsule, and a preparation method therefor and an application thereof. In a hydrophilic compound nano microcapsule provided by the present invention, a protein and polysaccharide serve as wall materials; the wall materials and a hydrophilic compound are mixed in water to form a mixed solution; and the pH of the mixed solution is adjusted to 3.4-4.5 while the mixed solution is subjected to ultrasonic treatment, such that the hydrophilic compound nano microcapsule is obtained. According to the preparation method provided by the present invention, the hydrophilic compound accounts for 0.5-7% of the total weight of dry substances of the wall materials.
The present invention provides a recombinant African swine fever virus capsid protein P72, a preparation method therefor, and an application thereof. The amino acid sequence of the recombinant protein is shown in SEQ ID NO:1. In the present invention, by means of structural analysis of the natural capsid protein P72 of the African swine fever virus and by using a mutation of an amino acid at a special site, the recombinant P72 protein can form a trimer structure without relying on auxiliary protein B602L, and same has a similar protein structure and immunogenicity to the natural P72 protein, and can be applied to preparing antibodies, vaccines, and detection products of the African swine fever virus.
The present application provides an SNP molecular marker affecting duck fatty acid composition-related gene ELOVL3, and the use thereof in breeding duck meat, identifying meat quality, etc., wherein the SNP molecular marker of the present application is located at -619 bp upstream of the ELOVL3 gene, and the genotype is AA, GG or GA. According to the present invention combined with a GWAS analysis and cell test, SNP located upstream of the ELOVL3 gene that affects the expression activity thereof is identified, and further detection determines that an AA-type individual has a higher polyunsaturated fatty acid carbon chain elongation ability than a GG-type individual, while a dominant genotype that is favorable for improving the fatty acid content of duck meat for seed retention can be selected.
Provided is an SNP site related to sebum on a CLN8 gene of a Peking duck, wherein the SNP site is selected from one or more of rs322493594, rs322493651, rs322493641, rs322493648 and rs322493619. Further provided are a detection kit of the sites and the use thereof in the detection or prediction of the cortical weight and/or cortical rate of the Peking duck and in the breeding of the Peking duck.
A gene ZmPLD3 for inducing maize maternal haploid production and its application thereof. Transgenic homozygous mutant plants or their progeny can be obtained by knocking out the ZmPLD3 gene in maize, and maize maternal haploids can be produced by hybridizing them as paternal materials with other maize materials. A series of allelic mutations of the gene having maternal haploid induction function through hybridization were obtained. The experiments showed that the mutation of maize phospholipase PLD3 could lead to the production of maize maternal haploid, which provides new thoughts for revealing the biological role of phospholipase in maize maternal haploid induction process. At the same time, the mutant individuals have the maternal haploid induction ability in maize, which is of great significance for breeding new types of haploid induction lines with high haploid induction rate and improving the efficiency of maize haploid breeding.
A method for inhibiting microbial clogging of drip irrigation emitters, belonging to the technical field of water-saving irrigation. The method includes the following steps: (1) Use developmental molecular ecological network analysis to determine the key bacteria causing the clogging of drip irrigation emitters, and then carry out screening of antagonistic bacteria to inhibit the clogging of drip irrigation emitters; (2) Ferment the selected antagonistic bacteria in a medium containing biogas slurry, to generate bacterial antagonist; (3) Apply the bacterial antagonist to drip irrigation system. The method uses the molecular ecological network analysis method to obtain key bacteria that cause the growth of biofilm in drip irrigation emitters, and then combines the principle of microbial antagonism to screen out the antagonistic bacteria that can antagonize the growth of biofilm, so as to inhibit the growth of biofilm in irrigation emitters.
A01G 25/02 - Watering arrangements located above the soil which make use of perforated pipe-lines or pipe-lines with dispensing fittings, e.g. for drip irrigation
patents
