The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield and/or titer of at least one protein of interest in a eukaryotic host cell, preferably a yeast, comprising overexpressing in said host cell a) at least one polynucleotide encoding at least one Sbh1 transport protein, and b) (i) at least one polynucleotide encoding at least one Kar2 endoplasmic reticulum helper protein; or (ii) at least one polynucleotide encoding at least one Hac transcription factor. The invention further relates to a recombinant eukaryotic host cell for manufacturing at least one protein of interest, which is engineered to overexpress at least one polynucleotide encoding said at least one Sbh1 transport protein, and b) (i) at least one polynucleotide encoding said at least one Kar2 endoplasmic reticulum helper protein; or (ii) at least one polynucleotide encoding said at least one Hac1 transcription factor as well as the use of said recombinant eukaryotic host cell for manufacturing at least one protein of interest.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield and/or titer of at least one protein of interest in a eukaryotic host cell, preferably a yeast, comprising overexpressing in said host cell a) at least one polynucleotide encoding at least one Sbh1 transport protein, and b) (i) at least one polynucleotide encoding at least one Kar2 endoplasmic reticulum helper protein; or (ii) at least one polynucleotide encoding at least one Hac1 transcription factor. The invention further relates to a recombinant eukaryotic host cell for manufacturing at least one protein of interest, which is engineered to overexpress at least one polynucleotide encoding said at least one Sbh1 transport protein, and b) (i) at least one polynucleotide encoding said at least one Kar2 endoplasmic reticulum helper protein; or (ii) at least one polynucleotide encoding said at least one Hac1 transcription factor as well as the use of said recombinant eukaryotic host cell for manufacturing at least one protein of interest.
The present invention relates to a nucleic acid molecule encoding a fusion protein comprising a secretion signal comprising (i) a signal peptide sequence originating from a KRE1 protein or a signal peptide sequence originating from a SWP1 protein; and optionally (ii) an α-mating factor (MFα) pro-sequence, and a protein of interest. The present invention further relates to a secretion signal as defined herein, an expression cassette comprising said nucleic acid molecule as well as recombinant eukaryotic host cells comprising said nucleic acid molecule or expression cassette. Further encompassed is a method of manufacturing a protein of interest in a eukaryotic host cell and a method of increasing the secretion of a protein of interest from a eukaryotic host cell. Further provided is the use of the secretion signal for increasing the secretion of a recombinant protein of interest from a eukaryotic host cell and the use of the recombinant host cell for manufacturing a recombinant protein of interest.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
The present invention relates to a nucleic acid molecule encoding a fusion protein comprising a secretion signal comprising (i) a signal peptide sequence originating from a KRE1 protein or a signal peptide sequence originating from a SWP1 protein; and optionally (ii) an ?-mating factor (MF?) pro-sequence, and a protein of interest. The present invention further relates to a secretion signal as defined herein, an expression cassette comprising said nucleic acid molecule as well as recombinant eukaryotic host cells comprising said nucleic acid molecule or expression cassette. Further encompassed is a method of manufacturing a protein of interest in a eukaryotic host cell and a method of increasing the secretion of a protein of interest from a eukaryotic host cell. Further provided is the use of the secretion signal for increasing the secretion of a recombinant protein of interest from a eukaryotic host cell and the use of the recombinant host cell for manufacturing a recombinant protein of interest.
The present invention relates to a nucleic acid molecule encoding a fusion protein comprising a secretion signal comprising (i) a signal peptide sequence originating from a KRE1 protein or a signal peptide sequence originating from a SWP1 protein; and optionally (ii) an α-mating factor (MFα) pro-sequence, and a protein of interest. The present invention further relates to a secretion signal as defined herein, an expression cassette comprising said nucleic acid molecule as well as recombinant eukaryotic host cells comprising said nucleic acid molecule or expression cassette. Further encompassed is a method of manufacturing a protein of interest in a eukaryotic host cell and a method of increasing the secretion of a protein of interest from a eukaryotic host cell. Further provided is the use of the secretion signal for increasing the secretion of a recombinant protein of interest from a eukaryotic host cell and the use of the recombinant host cell for manufacturing a recombinant protein of interest.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield of a protein of interest (POI) in a eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4/2. The invention relates further to a recombinant eukaryotic host cell for manufacturing a POI, wherein the host cell is engineered to overexpress at least one polynucleotide encoding at least one transcription factor as well as the use of the host cell for manufacturing a POI.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield of a protein of interest (POI) in a eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4/2. The invention relates further to a recombinant eukaryotic host cell for manufacturing a POI, wherein the host cell is engineered to overexpress at least one polynucleotide encoding at least one transcription factor as well as the use of the host cell for manufacturing a POI.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of increasing the yield of a protein of interest (POI) in a eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4/2. The invention relates further to a recombinant eukaryotic host cell for manufacturing a POI, wherein the host cell is engineered to overexpress at least one polynucleotide encoding at least one transcription factor as well as the use of the host cell for manufacturing a POI.
01 - Chemical and biological materials for industrial, scientific and agricultural use
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Chemical, biological, biochemical and biotechnological
preparations; biochemicals, in particular microorganisms, in
particular yeast and bacteria for use in protein expression;
microbial production strains for producing proteins, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; microbial production strains for producing plasmid
DNA; yeast strains, other than for making wine, medical or
veterinary use; bacterial strains, other than for making
wine, medical or veterinary use; proteins for use in
science; proteins, not for scientific purposes and medical
or veterinary use; proteins for use in industry, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; proteins, in particular enzymes, for use in
production processes, in particular for producing
foodstuffs, animal feed, chemicals, diagnostics, cosmetics,
pharmaceuticals and biopharmaceuticals; proteins for use in
industry, in particular for industrial, analytic and
diagnostic purposes and cleaning purposes; enzymes derived
from biotechnological processes for use in industry; nucleic
recombinant acids, in particular plasmid DNA; nucleic acids,
in particular deoxyribonucleic acids, in particular for
protein production. Consultancy in relation to biochemistry/biotechnology;
research and development in the field of
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and/or biological laboratory; conducting of biological and
biotechnological tests; biotechnological research relating
to industry; biotechnological research relating to enzymatic
synthesis; information on the subject of scientific research
in the field of biochemistry and biotechnology; working on
and evaluation of chemical syntheses; working on and
evaluation of chemical analyses; research and development in
the field of biochemistry and biotechnology, in particular
in relation to protein expression and protein production and
plasmid DNA production; research and development services
relating to science; development of proteins; scientific
research and development services, namely, production of
proteins, plasmid DNA production, plasmid DNA purification,
modification of proteins, purification of proteins, DNA
manipulation and cell banking services; development of
bioprocesses; strain development, in particular development
of production strains for producing plasmid DNA; enzyme
engineering; development of protein production processes;
development of processes for producing plasmid DNA; plasmid
DNA synthesis; development of methods for the purification
of plasmid DNA; development of protein purification methods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological
preparations; biochemicals, in particular microorganisms, in
particular yeast and bacteria for use in protein expression;
microbial production strains for producing proteins, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; microbial production strains for producing plasmid
DNA; yeast strains, other than for making wine, medical or
veterinary use; bacterial strains, other than for making
wine, medical or veterinary use; proteins for use in
science; proteins, not for scientific purposes and medical
or veterinary use; proteins for use in industry, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; proteins, in particular enzymes, for use in
production processes, in particular for producing
foodstuffs, animal feed, chemicals, diagnostics, cosmetics,
pharmaceuticals and biopharmaceuticals; proteins for use in
industry, in particular for industrial, analytic and
diagnostic purposes and cleaning purposes; enzymes derived
from biotechnological processes for use in industry; nucleic
recombinant acids, in particular plasmid DNA; nucleic acids,
in particular deoxyribonucleic acids, in particular for
protein production. Consultancy in relation to biochemistry/biotechnology;
research and development in the field of
chemistry/biochemistry/biotechnology; services of a chemical
and/or biological laboratory; conducting of biological and
biotechnological tests; biotechnological research relating
to industry; biotechnological research relating to enzymatic
synthesis; information on the subject of scientific research
in the field of biochemistry and biotechnology; working on
and evaluation of chemical syntheses; working on and
evaluation of chemical analyses; research and development in
the field of biochemistry and biotechnology, in particular
in relation to protein expression and protein production and
plasmid DNA production; research and development services
relating to science; development of proteins; scientific
research and development services, namely, production of
proteins, plasmid DNA production, plasmid DNA purification,
modification of proteins, purification of proteins, DNA
manipulation and cell banking services; development of
bioprocesses; strain development, in particular development
of production strains for producing proteins; strain
development, in particular development of production strains
for producing plasmid DNA; enzyme engineering; development
of protein production processes; development of processes
for producing plasmid DNA; plasmid DNA synthesis;
development of methods for the purification of plasmid DNA;
development of protein purification methods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological
preparations; biochemicals, in particular microorganisms, in
particular yeast and bacteria for use in protein expression;
microbial production strains for producing proteins, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; microbial production strains for producing plasmid
DNA; yeast strains, other than for making wine, medical or
veterinary use; bacterial strains, other than for making
wine, medical or veterinary use; proteins for use in
science; proteins, not for scientific purposes and medical
or veterinary use; proteins for use in industry, in
particular for the pharmaceutical and biopharmaceutical
industry, food industry, animal feed industry, chemical
industry, agricultural industry, diagnostics industry,
cosmetics industry and the laundry and cleaning preparations
industry; proteins, in particular enzymes, for use in
production processes, in particular for producing
foodstuffs, animal feed, chemicals, diagnostics, cosmetics,
pharmaceuticals and biopharmaceuticals; proteins for use in
industry, in particular for industrial, analytic and
diagnostic purposes and cleaning purposes; enzymes derived
from biotechnological processes for use in industry; nucleic
recombinant acids, in particular plasmid DNA; nucleic acids,
in particular deoxyribonucleic acids, in particular for
protein production. Consultancy in relation to biochemistry/biotechnology;
research and development in the field of
chemistry/biochemistry/biotechnology; services of a chemical
and/or biological laboratory; conducting of biological and
biotechnological tests; biotechnological research relating
to industry; biotechnological research relating to enzymatic
synthesis; information on the subject of scientific research
in the field of biochemistry and biotechnology; working on
and evaluation of chemical syntheses; working on and
evaluation of chemical analyses; research and development in
the field of biochemistry and biotechnology, in particular
in relation to protein expression and protein production and
plasmid DNA production; research and development services
relating to science; development of proteins; scientific
research and development services, namely, production of
proteins, plasmid DNA production, plasmid DNA purification,
modification of proteins, purification of proteins, DNA
manipulation and cell banking services; development of
bioprocesses; strain development, in particular development
of production strains for producing proteins; strain
development, in particular development of production strains
for producing plasmid DNA; enzyme engineering; development
of protein production processes; development of processes
for producing plasmid DNA; plasmid DNA synthesis;
development of methods for the purification of plasmid DNA;
development of protein purification methods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
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Chemical, biological, biochemical and biotechnological preparations for scientific and commercial purposes; Biochemicals, namely, microorganisms in the form of the yeast Komagataella phaffii known as Pichia pastoris for use in protein expression; Microbial production strains, namely, Komagataella phaffii known as Pichia pastoris for producing proteins, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Yeast strains, other than for making wine, medical or veterinary use; Proteins for use in science; Proteins, not for scientific purposes and medical or veterinary use; Proteins for use in industries, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Proteins, specifically enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for industrial use, in particular for industrial, analytic and diagnostic purposes and cleaning purposes; Enzymes derived from biotechnological processes for industrial use; all the aforementioned primarily consisting of Komagataella phaffii known as Pichia pastoris yeast Consultancy, namely, providing information on the subject of scientific research in the field of biochemistry and biotechnology; Research and development in the field of chemistry, biochemistry, and biotechnology; Chemical and biological laboratory services; Conducting of biological and biotechnological tests for research and commercial purposes; Industrial biotechnological research; Biotechnological research in the field of enzymatic synthesis; Working on and evaluation of chemical syntheses; Working on and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production; Research and development services relating to biotechnical biochemistry science; Development of proteins for use in pharmaceuticals, biotechnology, and medical and veterinary diagnostics; Development of bioprocesses for protein production; Strain development services, namely development of production strains for producing proteins; Enzyme engineering for others; Development of protein production processes for others; Development of protein purification methods; all the aforementioned in the field of Komagataella phaffii known as Pichia pastoris yeast
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological preparations, namely, for scientific and commercial purposes; biochemicals, namely, microorganisms in the form of, yeast and bacteria for use in protein expression; microbial production strains, namely, Komagataella phaffii strains known as Pichia pastoris and Escherichia coli strains for producing proteins, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries, and the laundry and cleaning preparations industries; microbial production strains for producing plasmid DNA; yeast strains, other than for making wine, medical or veterinary use; bacterial strains, other than for making wine, medical or veterinary use; proteins for use in science; proteins, not for scientific purposes and medical or veterinary use; proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries, and the laundry and cleaning preparations industries; proteins specifically, enzymes, for use in production processes, namely, for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; proteins for industrial use in particular for industrial, analytic and diagnostic purposes and cleaning purposes; enzymes derived from biotechnological processes for industrial use; nucleic recombinant acids, in particular plasmid DNA, for laboratory use; nucleic acids, in particular deoxyribonucleic acids, in particular for protein production Consultancy, namely, providing information on the subject of scientific research in the field of biochemistry and biotechnology; research and development in the field of chemistry, biochemistry, and biotechnology; chemical and biological laboratory services; conducting of biological and biotechnological tests for commercial and scientific purposes; industrial biotechnological research; biotechnological research in the field of enzymatic synthesis; providing information on the subject of scientific research in the field of biochemistry and biotechnology; working on and evaluation of chemical syntheses; working on and evaluation of chemical analyses; research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; research and development services relating to biotechnical biochemistry science; development of proteins for use in pharmaceuticals, biotechnology, and medical and veterinary diagnostics; scientific research and development services, namely, production of proteins, plasmid DNA production, plasmid DNA purification, modification of proteins, purification of proteins, DNA manipulation and cell banking services for others; development of bioprocesses for protein production and plasmid DNA production; strain development services, namely, development of production strains for producing proteins; strain development, in particular the development of production strains for producing plasmid DNA; enzyme engineering for others; development of protein production processes for others; development of processes for producing plasmid DNA for others; plasmid DNA synthesis for others; development of plasmid DNA purification methods; development of protein purification methods
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological preparations, namely, for scientific and commercial purposes; biochemicals, namely, microorganisms in the form of, yeast and bacteria for use in protein expression; microbial production strains, namely, Komagataella phaffii strains known as Pichia pastoris and Escherichia coli strains for producing proteins, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries, and the laundry and cleaning preparations industries; microbial production strains for producing plasmid DNA; yeast strains, other than for making wine, medical or veterinary use; bacterial strains, other than for making wine, medical or veterinary use; proteins for use in science; proteins, not for scientific purposes and medical or veterinary use; proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries, and the laundry and cleaning preparations industries; proteins specifically, enzymes, for use in production processes, namely, for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; proteins for industrial use in particular for industrial, analytic and diagnostic purposes and cleaning purposes; enzymes derived from biotechnological processes for industrial use; nucleic recombinant acids, in particular plasmid DNA, for laboratory use; nucleic acids, in particular deoxyribonucleic acids, in particular for protein production Consultancy, namely, providing information on the subject of scientific research in the field of biochemistry and biotechnology; research and development in the field of chemistry, biochemistry, and biotechnology; chemical and biological laboratory services; conducting of biological and biotechnological tests for commercial and scientific purposes; industrial biotechnological research; biotechnological research in the field of enzymatic synthesis; providing information on the subject of scientific research in the field of biochemistry and biotechnology; working on and evaluation of chemical syntheses; working on and evaluation of chemical analyses; research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; research and development services relating to biotechnical biochemistry science; development of proteins for use in pharmaceuticals, biotechnology, and medical and veterinary diagnostics; scientific research and development services, namely, production of proteins, plasmid DNA production, plasmid DNA purification, modification of proteins, purification of proteins, DNA manipulation and cell banking services for others; development of bioprocesses for protein production and plasmid DNA production; strain development services, namely, development of production strains for producing proteins; strain development, in particular the development of production strains for producing plasmid DNA; enzyme engineering for others; development of protein production processes for others; development of processes for producing plasmid DNA for others; plasmid DNA synthesis for others; development of plasmid DNA purification methods; development of protein purification methods
01 - Chemical and biological materials for industrial, scientific and agricultural use
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
(1) Chemical, biological, biochemical and biotechnological preparations for scientific and commercial purposes; Biochemicals, in particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains, namely, yeast and bacteria for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Microbial production strains for producing plasmid DNA; Yeast for scientific and commercial purposes; Bacterial strains; Proteins for use in biotechnical biochemistry science; Proteins, not for scientific purposes for scientific and commercial purposes; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industry, for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Enzymes derived from biotechnological processes for use in industry; Nucleic recombinant acids, In particular plasmid DNA; Nucleic acids, In particular deoxyribonucleic acids, in particular for protein production (1) Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Processing and evaluation of chemical syntheses; Processing and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; Research and development services relating to biotechnical biochemistry science; DNA manipulation for scientific research and commercial purposes; Development of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Production of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Plasmid DNA production; Modification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Development of bioprocesses for protein production and plasmid DNA production; Strain development, in particular development of production strains for producing proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Strain development, in particular development of production strains for producing plasmid DNA; Purification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Plasmid DNA purification; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Development of processes for producing plasmid DNA; Plasmid DNA synthesis; Development of methods for the purification of plasmid DNA; Development of protein purification methods in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries
01 - Chemical and biological materials for industrial, scientific and agricultural use
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
(1) Chemical, biological, biochemical and biotechnological preparations for scientific and commercial purposes; Biochemicals, in particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains, namely, Komagataella phaffii known as Pichia pastoris for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Yeast for scientific and commercial purposes; Proteins for use in biotechnical biochemistry science; Proteins, not for scientific purposes and other than for medical or veterinary use; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industries, in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Enzymes derived from biotechnological processes for use in industry; Nucleic acids, in particular deoxyribonucleic acids, in particular for protein production (1) Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Processing and evaluation of chemical syntheses; Processing and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production; Research and development services relating to biotechnical biochemistry science; DNA manipulation for scientific research and commerical purposes; Development of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Production of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Modification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Development of bioprocesses for protein production; Strain development, in particular development of production strains for producing proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Purification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Development of protein purification methods in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries
01 - Chemical and biological materials for industrial, scientific and agricultural use
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
44 - Medical, veterinary, hygienic and cosmetic services; agriculture, horticulture and forestry services
Goods & Services
(1) Chemical, biological, biochemical and biotechnological preparations for scientific and commercial purposes; Biochemicals, in particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains, namely, yeast and bacteria for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Microbial production strains for producing plasmid DNA; Yeast for scientific and commercial purposes; Bacterial strains; Proteins for use in biotechnical biochemistry science; Proteins, not for scientific purposes for scientific and commercial purposes; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industry, for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Enzymes derived from biotechnological processes for use in industry; Nucleic recombinant acids, In particular plasmid DNA; Nucleic acids, In particular deoxyribonucleic acids, in particular for protein production (1) Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Processing and evaluation of chemical syntheses; Processing and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; Research and development services relating to biotechnical biochemistry science; DNA manipulation for scientific research and commercial purposes; Development of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Production of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Plasmid DNA production; Modification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Development of bioprocesses for protein production and plasmid DNA production; Strain development, in particular development of production strains for producing proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Strain development, in particular development of production strains for producing plasmid DNA; Purification of proteins for use in pharmaceutical and biotechnological preparations, food preparations, animal feed preparations, chemical preparations, agricultural preparations, diagnostic preparations, cosmetic preparations, laundry preparations and medical and veterinary diagnostics; Plasmid DNA purification; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries; Development of processes for producing plasmid DNA; Plasmid DNA synthesis; Development of methods for the purification of plasmid DNA; Development of protein purification methods in particular for the pharmaceutical and biopharmaceutical industries, food industries, animal feed industries, chemical industries, agricultural industries, diagnostics industries, cosmetics industries and the laundry and cleaning preparations industries
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological preparations; Biochemicals, In particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Yeast strains; Proteins for use in science; Proteins, not for scientific purposes; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industry, in particular for industrial, analytic and diagnostic purposes and cleaning purposes; Enzymes derived from biotechnological processes for use in industry; Nucleic acids, in particular deoxyribonucleic acids, in particular for protein production. Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and/or biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Working on and evaluation of chemical syntheses; Working on and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production; Research and development services relating to science; DNA manipulation; Development of proteins; Production of proteins; Modification of proteins; Development of bioprocesses; Strain development, in particular development of production strains for producing proteins; Purification of proteins; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes; Development of protein purification methods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological preparations; Biochemicals, In particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Microbial production strains for producing plasmid DNA; Yeast strains; Bacterial strains; Proteins for use in science; Proteins, not for scientific purposes; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industry, in particular for industrial, analytic and diagnostic purposes and cleaning purposes; Enzymes derived from biotechnological processes for use in industry; Nucleic recombinant acids, In particular plasmid DNA; Nucleic acids, in particular deoxyribonucleic acids, in particular for protein production. Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and/or biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Working on and evaluation of chemical syntheses; Working on and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; Research and development services relating to science; DNA manipulation; Development of proteins; Production of proteins; Plasmid DNA production; Modification of proteins; Development of bioprocesses; Strain development, in particular development of production strains for producing proteins; Strain development, in particular development of production strains for producing plasmid DNA; Purification of proteins; Plasmid DNA purification; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes; Development of processes for producing plasmid DNA; Plasmid DNA synthesis; Development of methods for the purification of plasmid DNA; Development of protein purification methods.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Chemical, biological, biochemical and biotechnological preparations; Biochemicals, In particular microorganisms, in particular yeast and bacteria for use in protein expression; Microbial production strains for producing proteins, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Microbial production strains for producing plasmid DNA; Yeast strains; Bacterial strains; Proteins for use in science; Proteins, not for scientific purposes; Proteins for use in industry, in particular for the pharmaceutical and biopharmaceutical industry, food industry, animal feed industry, chemical industry, agricultural industry, diagnostics industry, cosmetics industry and the laundry and cleaning preparations industry; Proteins, in particular enzymes, for use in production processes, in particular for producing foodstuffs, animal feed, chemicals, diagnostics, cosmetics, pharmaceuticals and biopharmaceuticals; Proteins for use in industry, in particular for industrial, analytic and diagnostic purposes and cleaning purposes; Enzymes derived from biotechnological processes for use in industry; Nucleic recombinant acids, In particular plasmid DNA; Nucleic acids, In particular deoxyribonucleic acids, in particular for protein production. Consultancy in relation to biochemistry/biotechnology; Research and development in the field of chemistry/biochemistry/biotechnology; Services of a chemical and/or biological laboratory; Conducting of biological and biotechnological tests; Biotechnological research relating to industry; Biotechnological research relating to enzymatic synthesis; Information on the subject of scientific research in the field of biochemistry and biotechnology; Working on and evaluation of chemical syntheses; Working on and evaluation of chemical analyses; Research and development in the field of biochemistry and biotechnology, in particular in relation to protein expression and protein production and plasmid DNA production; Research and development services relating to science; DNA manipulation; Development of proteins; Production of proteins; Plasmid DNA production; Modification of proteins; Development of bioprocesses; Strain development, in particular development of production strains for producing proteins; Strain development, in particular development of production strains for producing plasmid DNA; Purification of proteins; Plasmid DNA purification; Services in relation to cell banking systems; Enzyme engineering; Development of protein production processes; Development of processes for producing plasmid DNA; Plasmid DNA synthesis; Development of methods for the purification of plasmid DNA; Development of protein purification methods.
24.
RECOMBINANT HOST CELL WITH ALTERED MEMBRANE LIPID COMPOSITION
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
C07K 14/39 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from fungi from yeasts
C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
C12N 9/16 - Hydrolases (3.) acting on ester bonds (3.1)
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
C12N 15/67 - General methods for enhancing the expression
C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
27.
RECOMBINANT HOST CELL FOR EXPRESSING PROTEINS OF INTEREST
The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
A mutant Pichia pastoris alcohol oxidase 1 (AOXI) promoter of the wild type Pichia pastoris AOXI promoter (SEQ ID No. 1) comprising at least one mutation selected from the group consisting of : a) a transcription factor binding site (TFBS), b) nucleotides 170 to 235 (-784 to -719), nucleotides 170 to 191 (-784 to -763), nucleotides 192 to 213 (-762 to -741), nucleotides 192 to 210 (-762 to -744), nucleotides 207 to 209 (-747 to -745), nucleotides 214 to 235 (-740 to -719), nucleotides 304 to 350 (-650 to -604), nucleotides 364 to 393 (-590 to -561), nucleotides 434 to 508 (-520 to -446), nucleotides 509 to 551 (-445 to -403), nucleotides 552 to 560 (-402 to -394), nucleotides 585 to 617 (-369 to -337), nucleotides 621 to 660 (-333 to -294), nucleotides 625 to 683 (-329 to -271), nucleotides 736 to 741 (-218 to -213), nucleotides 737 to 738 (-217 to -216); nucleotides 726 to 755 (-228 to -199), nucleotides 784 to 800 (-170 to -154) or nucleotides 823 to 861 (-131 to -93) of Seq ID No. 1, and combinations thereof.
