Provided herein is a method of increasing production of a dihydrodiphenylpropanoid derivative compound (e.g., dihydrostilbenoid or dihydrochalcone compound) in a recombinant host cell. The method comprising: reducing or eliminating expression of a gene encoding a double-bond reductase polypeptide or activity of the double-bond reductase polypeptide encoded thereby; expressing a recombinant gene encoding a double-bond reductase selected from the group consisting of a first polypeptide, second polypeptide, third polypeptide, and combinations thereof. The first polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 3, the second polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 5, and the third polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 7.
A strategy to produce short peptides through tandem repetitive recombinant protein has been developed as disclosed herein. Furthermore, the production of tandem repetitive recombinant proteins in Pichia pastoris and optimized protease cleavage conditions for purification of bioactive peptides has been improved using techniques disclosed herein. Provided herein is the production of flavor and cosmetics peptides, and the technology is applied to production of various short peptides biologically. These techniques incorporate novel applications of signal peptides, design spacers for generating tandem repetitive recombinant protein and increasing copy number, co-expression of chaperones and optimization of protease cleavage conditions. Specifically, provided herein are methods using designed tandem repeated genes, optimized gene spacers and signal peptides useful for improved production of short peptide in Pichia and E. coli. These novel applications afford improvements in short peptide production and high efficiency biological production of bioactive peptides.
Provided herein is a method of producing hydroxytyrosol. The method includes: culturing a recombinant host microorganism in a medium, wherein: the host microorganism has been transformed with: a first polynucleotide sequence encoding a decarboxylase enzyme capable of decarboxylating 4 hydroxyphenylpyruvaic acid (HPP) to produce 4 hydroxyphenylacetaldehyde, and a second polynucleotide sequence encoding a hydroxylase enzyme, wherein the hydroxylase has an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 2, and the medium comprises glucose; and collecting product hydroxytyrosol from one or both of the host microorganism and the culture medium.
Provided herein are biosynthetic methods for producing brazzein and related compositions. Uses (e.g., as sweeteners) of the brazzein produced using the methods are also provided.
The present disclosure relates to methods and materials for the production of resveratrol in yeasts (e.g., yeast of the Saccharomyces genus such as S. cerevisiae) by increasing expression of genes of the pentose phosphate or Shikimate pathway and others, such genes, for example, encoding acetyl-CoA synthase, transketolase, transaldolase, enolase, aromatic aminotransferase, prephenate dehydratase, ribose-5-phosphate isomerase, or a monocarboxylate permease.
The present disclosure provides polymers prepared by a method comprising reacting a polymerization mixture comprising one or more types of hydroxy/amino-oximes, one or more types of diols, one or more types of polyols (e.g., triols), one or more types of isocyanates, and optionally one or more types of oximes. At least one instance of =N—OH of at least one instance of the hydroxy/amino-oximes may react with —NCO of the isocyanates to form ═N—O—C(═O)—NH— bonds. The polymers may be a thermoset under ambient conditions. At elevated temperatures, the polymers may undergo a transition from a cross-linked state to a lightly cross-linked or un-crosslinked state (e.g., at least in part because at least one instance of the =N—O—C(═O)—NH— bonds may be cleaved (e.g., to form ═N—OH and —NCO)). The transition may be reversable (e.g., by changing the temperature). The polymers of the present disclosure may be useful as hot melt adhesives. The present disclosure also provides compositions, kits, methods of preparation, methods of bonding, and methods of de-bonding.
C08G 18/67 - Unsaturated compounds having active hydrogen
C08G 18/76 - Polyisocyanates or polyisothiocyanates cyclic aromatic
C09J 5/06 - Adhesive processes in generalAdhesive processes not provided for elsewhere, e.g. relating to primers involving heating of the applied adhesive
C09J 175/14 - Polyurethanes having carbon-to-carbon unsaturated bonds
7.
ESTERS OF A RETINOID AND BAKUCHIOL AND PREPARATIONS THEREOF
The present disclosure provides esters of a retinoid and bakuchiol, such as esters of Formula (I′). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
The present disclosure provides esters of a retinoid and bakuchiol, such as esters of Formula (I′). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
C07C 403/20 - Derivatives of cyclohexane or of a cyclohexene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by carboxyl groups
Provided herein are biosynthetic methods for producing brazzein and related compositions. Uses (e.g., as sweeteners) of the brazzein produced using the methods are also provided. Brazzein is expressed in yeast as tandem repeats with promoters, terminators and coexpressing chaperones.
The present disclosure relates, at least in part, to the production of delta-lactones and gamma-lactones from fatty acid substrates (e.g., fatty acid substrates of C16-C25) via a batch fermentation method in an engineered microbe using a recombinant cytochrome P450 monooxygenase.
The present disclosure relates, at least in part, to the production of delta-lactones from fatty acid substrates via a batch fermentation method in an engineered microbe using a recombinant cytochrome P450 monooxygenase.
The present invention relates to engineered microbial host cells comprising exogenous genes coding for proteins responsible for converting histidine and cysteine into ergothioneine in greater efficiency than the wild-type cells. Also provided in this invention are methods for producing ergothioneine using the engineered microbial host cells of the present invention.
The present invention relates to novel steviol glycosides rebaudioside R6-5, rebaudioside R6-6, and rebaudioside R7-5 and the production of these novel steviol glycosides, such as through enzymatic bioconversion.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C07H 13/08 - Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals directly attached to carbocyclic rings
13.
BIOSYNTHETIC PRODUCTION OF MACROCYCLIC MUSK LACTONES FROM FATTY ACIDS
Provided herein are biosynthetic methods for producing lactones, such as macrocyclic lactones, from fatty acids. The lactones produced using and/or during the biosynthetic methods are also provided.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A21D 13/062 - Products with modified nutritive value, e.g. with modified starch content with modified sugar contentSugar-free products
A23G 4/10 - Chewing gum characterised by the composition characterised by the carbohydrates used, e.g. polysaccharides
C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
C12N 15/74 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
15.
NOVEL TRYPTOPHANASES FROM STREPTOMYCES WITH NOVEL PROPERTIES AND THE USES THEREOF
The present disclosure is generally directed to a putative tryptophanase homolog from Streptomyces hygroscopicus expressed in E. coli. The expressed protein showed useful biochemical properties. It catalyzed the conversion of 6-bromotryptophan to 6-bromo-indole, but unlike the reported tryptophanase, it had no activity in converting tryptophan to indole. This biochemical property is applicable in Tyrian purple production.
The present disclosure relates, at least in part, to the production of ambroxide or a derivative thereof from homofamesol. The production can be mediated in a cellular system (e.g., an Escherichia coli bacterium) or in an enzymatic reaction mixture without a cellular system.
Provided herein is a method of increasing production of a dihydrodiphenylpropanoid ddeerriivvaattiivvee compound (e.g., dihydrostilbenoid or dihydrochalcone compound) in a recombinant host cell. The method comprising: reducing or eliminating expression of a gene encoding a double-bond reductase polypeptide or activity of the double-bond reductase polypeptide encoded thereby; expressing a recombinant gene encoding a double -bond reductase selected from the group consisting of a first polypeptide, second polypeptide, third polypeptide, and combinations thereof. The first polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 3, the second polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 5, and the third polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 7.
The present disclosure provides a method of carbocyclyl moiety formation across a carbon-carbon multiple bond. The method comprises combining components comprising: a substrate compound comprising a carbon-carbon multiple bond; a diazo alkylation reagent; a cyclopropanation catalyst comprising a transition metal selected from the group consisting of Pd, Pt, Cu, and Ru, and a catalyst enhancer comprising Ni, to form a reaction mixture for producing a product compound comprising a cyclopropyl moiety.
C07C 45/69 - Preparation of compounds having C=O groups bound only to carbon or hydrogen atomsPreparation of chelates of such compounds by reactions not involving the formation of C=O groups by isomerisationPreparation of compounds having C=O groups bound only to carbon or hydrogen atomsPreparation of chelates of such compounds by reactions not involving the formation of C=O groups by change of size of the carbon skeleton by increase in the number of carbon atoms by addition to carbon-to-carbon double or triple bonds
C07C 13/04 - Monocyclic hydrocarbons or acyclic hydrocarbon derivatives thereof with a three-membered ring
C07D 307/77 - Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
A method for synthesizing salidroside, the method comprising: (i) preparing a reaction mixture comprising: tyrosol, uridine diphosphate-glucose (UDP-glucose), and a uridine diphosphate (UDP)-glycosyltransferase, and (ii) incubating the reaction mixture to produce salidroside, wherein a glucose is covalently coupled to the tyrosol to produce salidroside. The UDP- glycosyltransferase is selected from the group consisting of a first polypeptide, a second polypeptide, a third polypeptide, and combinations thereof. The first polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 5, the second polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 11, and the third polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 15.
A23L 2/00 - Non-alcoholic beveragesDry compositions or concentrates thereforPreparation or treatment thereof
A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
The present invention provides a novel biosynthetic production process which converts L-galactose into 2′-fucosyllactose via four enzymatically catalyzed reaction steps. The present process is designed such that co-factors required by the process are regenerated within the four reaction steps, hence making the process cost-effective and efficient. The process can be performed in vitro in a cell-free system. The present invention also provides mutant enzymes that can be used to increase production levels of 2′-fucosyllactose, whether using the novel pathway described herein or the mannose-dependent pathway known in the art.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
The present invention relates to microbial production of nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), and nicotinamide adenine dinucleotide (NAD) using a genetically modified bacterium.
The present invention relates to a compound of Formula (I) and/or a physiologically acceptable salt, enantiomer, or solvate thereof. Said compound can be used in combination with one or more sweeteners to enhance their individual sweet taste or the overall sweet taste of a composition comprising said one or more sweeteners.
A23L 27/00 - SpicesFlavouring agents or condimentsArtificial sweetening agentsTable saltsDietetic salt substitutesPreparation or treatment thereof
A23L 29/00 - Foods or foodstuffs containing additivesPreparation or treatment thereof
A23L 33/125 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing carbohydrate syrupsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugarsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing sugar alcoholsModifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives containing starch hydrolysates
25.
RESILIN-SILICA BINDING DOMAIN FUSION PROTEINS FOR BIOMATERIAL FORMATION
The present application relates, at least in part, to fusion proteins comprising (i) a resilin protein and (ii) a silicon oxide-binding domain. Also included are vectors, cell systems, and methods for making the fusion proteins, as well as composite materials comprising same.
C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Provided herein are methods for identifying and isolating polynucleotides coding for polypeptides having D-allulose 3-epimerase activity from a wide variety of microorganisms. Also provided are nucleic acid constructs, vectors and recombinant host cells comprising the polynucleotides coding for D-allulose 3-epimerase activity as well as methods for producing allulose from fructose using said recombinant host cells having D-allulose 3-epimerase activity or the D-allulose 3-epimerase enzyme of said recombinant host cells having D-allulose 3-epimerase activity.
e.g.e.g.e.g.e.g., by changing the temperature). The polymers of the present disclosure may be useful as hot melt adhesives. The present disclosure also provides compositions, kits, methods of preparation, methods of bonding, and methods of de-bonding.
C08G 18/28 - Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen characterised by the compounds used containing active hydrogen
e.g.e.g.e.g.e.g.e.g., by changing the temperature). The polymers of the present disclosure may be useful as hot melt adhesives. The present disclosure also provides compositions, kits, methods of preparation, methods of bonding, and methods of de-bonding.
C08G 18/28 - Polymeric products of isocyanates or isothiocyanates with compounds having active hydrogen characterised by the compounds used containing active hydrogen
C08G 18/12 - Prepolymer processes involving reaction of isocyanates or isothiocyanates with compounds having active hydrogen in a first reaction step using two or more compounds having active hydrogen in the first polymerisation step
C08G 18/24 - Catalysts containing metal compounds of tin
C08G 18/38 - Low-molecular-weight compounds having hetero atoms other than oxygen
The present disclosure provides polymers prepared by a method comprising reacting a polymerization mixture comprising one or more types of diols, one or more types of polyols, and one or more types of isocyanates, wherein at least one type of the diols and/or at least one type of the polyols are phenols, wherein each of the phenols is independently a diol or polyol, wherein one or more instances of -OH, -NH2, and/or -NH- are aryl-bound. At least one instance of C- OH of at least one instance of the diols or polyols may react with -NCO of the isocyanates to form C-O-C(=O)-NH- bonds. The polymers may be a thermoset under ambient conditions. At elevated temperatures, the polymers may undergo a transition from a cross-linked state to a lightly cross-linked or un-crosslinked state (e.g., at least in part because at least one instance of the C-O-C(=O)-NH- bonds may be cleaved (e.g., to form C-OH and -NCO)). The transition may be reversable (e.g., by changing the temperature). The polymers of the present disclosure may be useful as hot melt adhesives. The present disclosure also provides compositions, kits, methods of preparation, methods of bonding, and methods of de-bonding.
The present invention relates, at least in part, to compounds and compositions that can be used to mask, block, or reduce the bitter taste present in various orally consumable products. The present invention also relates to methods of using bitterness blocking compounds and compositions to mask the bitterness of various orally consumable products, hence making such orally consumable products more palatable. The present invention further relates to an orally consumable product with reduced bitterness.
Provided herein is a method of producing hydroxytyrosol. The method includes, in some embodiments: culturing a recombinant host microorganism in a medium, wherein: the host microorganism has been transformed with: a first polynucleotide sequence encoding a decarboxylase enzyme which is phenylpyruvate decarboxylase (EC 4.1.1.43) capable of decarboxylating 4-hydroxyphenylpyruvaic acid (HPP) to produce 4 hydroxyphenylacetaldehyde, and a second polynucleotide sequence encoding a hydroxylase enzyme which is 4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.14.9), wherein the hydroxylase has an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 2, and the medium comprises glucose; and collecting product hydroxytyrosol from one or both of the host microorganism and the culture medium.
Provided herein are biosynthetic methods for producing brazzein and related compositions employing expression in Pichia pastoris using vectors with tandem repeat brazzein sequences with spacers that mimics yeast alpha mating factor and regulatory sequences for enhanced expression. Uses (e.g., as sweeteners) of the brazzein produced using the methods are also provided.
C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
C12N 15/66 - General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligationUse of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
C12N 15/67 - General methods for enhancing the expression
Provided herein are methods for improving the biosynthetic production of PQQ in Methylopila sp, YHT-1 strain using genetic engineering approaches to increase the copy numbers and the expression levels of the PQQ biosynthetic genes.
A recombinant polypeptide comprising an alpha hydroxylase (TouA) subunit of a toluene-o-xylene monooxygenase (ToMO) polypeptide, wherein the TouA subunit is expressed from a gene having at least 95% sequence identity with the nucleotide sequence of SEQ ID NO: 2, wherein the TouA subunit expressed comprises at least one mutation compared to the amino acid sequence of SEQ ID NO: 1, wherein said at least one mutation is selected from the group consisting of F205A, F205C, F205D, F205E, F205K, F205M, F205N, F205P, F205Q, F205R, F205T, and F205V.
Provided herein are synthetic/recombinant nucleic acid molecules, nucleic acid constructs, enzymes, fusion enzymes, transformed host cells, and methods for making vitamin A compounds retinal, retinol and retinyl esters.
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
C07K 14/195 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria
The present disclosure provides esters of a retinoid and an alcohol selected from the group consisting of tocopherols and tert-butylhydroquinone, such as esters of Formula (I). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, and methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
The present disclosure provides esters of a retinoid and an alcohol selected from the group consisting of tocopherols and tert-butylhydroquinone, such as esters of Formula (I). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, and methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
The present disclosure provides esters of a retinoid and an alcohol selected from the group consisting of tocopherols and tert-butyl hydroquinone, such as esters of Formula (I). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, and methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
C07C 69/618 - Esters of carboxylic acids having a carboxyl group bound to an acyclic carbon atom and having a six-membered aromatic ring in the acid moiety having unsaturation outside the six-membered aromatic ring
C07D 311/74 - Benzo [b] pyrans, hydrogenated in the carbocyclic ring
The present disclosure provides esters of a retinoid and bakuchiol, such as esters of Formula (I'). The present disclosure also provides compositions and kits comprising the esters, methods of producing the esters, methods of using the esters (e.g., for treating or preventing a skin disease, slowing the ageing of the skin, improving the appearance of the skin, or modulating a retinoid receptor).
C07C 403/20 - Derivatives of cyclohexane or of a cyclohexene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by carboxyl groups
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY (USA)
Inventor
Attanayake, Gayanthi
Mao, Guohong
Walker, Kevin, D.
Abstract
The present disclosure provides methods of producing substituted pyrazines. The methods comprise, in some embodiments, incubating a certain a-keto-acid, a certain aldehyde, and a pyruvate decarboxylase to form a certain a-hydroxyketone. The methods may further comprise incubating the certain a-hydroxyketone with a certain amine to form a certain substituted dihydropyrazine. The methods may further comprise incubating the certain substituted dihydropyrazine, a base, and an oxidant to form a certain substituted pyrazine. The substituted pyrazines produced by the methods may be regioselectively enriched.
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
43.
BIOSYNTHETIC PRODUCTION OF STEVIOL GLYCOSIDE REBAUDIOSIDE I VIA VARIANT ENZYMES
The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A23L 27/10 - Natural spices, flavouring agents or condimentsExtracts thereof
A61K 9/00 - Medicinal preparations characterised by special physical form
C07C 233/18 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
C07C 233/20 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton
The present disclosure provides a method of preparing certain 3,4-dihydro-4-aryl coumarin derivatives. Cinnamic acid, or a certain derivative thereof, may react with phenol, or a certain derivative thereof (e.g., resorcinol), with removal of water azeotropically in the presence of a catalyst and an organic solvent to form a certain 3,4-dihydro-4-aryl coumarin derivative. The present disclosure also provides certain novel 3,4-dihydro-4-aryl coumarin derivatives, and compositions, kits, and methods of use thereof.
C07D 311/20 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 hydrogenated in the hetero ring
A61P 31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
The present disclosure relates, at least in part, to the production of delta-lactones from fatty acid substrates via a batch fermentation method in an engineered microbe using a recombinant cytochrome P450 monooxygenase.
The present invention relates to novel steviol glycosides rebaudioside R6-5, rebaudioside R6-6, and rebaudioside R7-5 and the production of these novel steviol glycosides, such as through enzymatic bioconversion.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
Provided herein are recombinant nucleic acid molecules, nucleic acid constructs, fusion enzymes, transformed host cells, and methods for making aroma compounds alpha-ionone or beta-ionone.
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
The present invention relates to an improved method to produce isosteviol by acid hydrolysis using sulfuric acid. The use of isosteviol in the field of medicinal chemistry is also provided.
C07C 62/24 - Saturated compounds containing keto groups the keto group being part of a ring
C07C 51/373 - Preparation of carboxylic acids or their salts, halides, or anhydrides by reactions not involving formation of carboxyl groups by introduction of functional groups containing oxygen only in doubly bound form
53.
BIOSYNTHETIC PRODUCTION OF MACROCYCLIC MUSK LACTONES FROM FATTY ACIDS
Provided herein are biosynthetic methods for producing lactones, such as macrocyclic lactones, from fatty acids. The lactones produced using and/or during the biosynthetic methods are also provided.
The present invention relates, in some aspects, to the production of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some aspects, the invention relates to mutant CP1 enzymes, mutant HV1 enzymes as well as host cells and methods utilizing such enzymes, such as to produce rebaudioside D4.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The present disclosure relates to engineered microbial host cells comprising exogenous genes coding for proteins responsible for converting histidine and cysteine into ergothioneine in greater efficiency than the wild-type cells. Also provided in this disclosure are methods for producing ergothioneine using the engineered microbial host cells of the present disclosure.
The present invention relates, at least in part, to the production of resveratrol from 4-coumaric acid. The production can be mediated in a transgenic Saccharomyces cell.
The present invention relates to a compound of Formula (I) and/or a physiologically acceptable salt, enantiomer, or solvate thereof. Said compound can be used in combination with one or more sweeteners to enhance their individual sweet taste or the overall sweet taste of a composition comprising said one or more sweeteners.
The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
The present invention relates to the production of vanillin via the bioconversion of isoeugenol. The bioconversion can be mediated in a cellular system (e.g., an Escherichia coli bacterium), or in an enzymatic reaction mixture without a cellular system.
The present invention relates to the production of vanillin via the bioconversion of isoeugenol. The bioconversion can be mediated in a cellular system (e.g., an Escherichia coli bacterium), or in an enzymatic reaction mixture without a cellular system.
Provided herein are methods for making lactones comprising incubating a cellular system expressing a heterologous cytochrome P450 (CYP450) protein with a medium comprising straight chain fatty acids to produce subterminal hydroxy fatty acids, and incubating a yeast cell culture with the subterminal hydroxy fatty acids to produce lactones.
The present disclosure relates to the biosynthesis of UDP-Rhamnose and recombinant polypeptides having enzymatic activity useful in the relevant biosynthetic pathways for producing UDP-Rhamnose. The present invention also provides a method for preparing a steviol glycoside composition comprising at least one rhamnose-containing steviol glycoside.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 9/04 - Oxidoreductases (1.), e.g. luciferase acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
C12N 15/52 - Genes encoding for enzymes or proenzymes
in vitro in vitro in a cell-free system. The present invention also provides mutant enzymes that can be used to increase production levels of 2'-fucosyllactose, whether using the novel pathway described herein or the mannose-dependent pathway known in the art.
C07K 14/34 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Corynebacterium (G)
C12N 15/52 - Genes encoding for enzymes or proenzymes
C12N 15/77 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for CorynebacteriumVectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Brevibacterium
65.
BIOSYNTHESIS PRODUCTION OF STEVIOL GLYCOSIDES AND PROCESSES THEREFORE
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C07H 15/24 - Condensed ring systems having three or more rings
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/44 - Preparation of O-glycosides, e.g. glucosides
A21D 13/062 - Products with modified nutritive value, e.g. with modified starch content with modified sugar contentSugar-free products
A23G 4/10 - Chewing gum characterised by the composition characterised by the carbohydrates used, e.g. polysaccharides
C07H 1/00 - Processes for the preparation of sugar derivatives
The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
Provided herein are methods for identifying and isolating polynucleotides coding for polypeptides having D-allulose 3-epimerase activity from a wide variety of microorganisms. Also provided are nucleic acid constructs, vectors and recombinant host cells comprising the polynucleotides coding for D-allulose 3-epimerase activity as well as methods for producing allulose from fructose using said recombinant host cells having D-allulose 3-epimerase activity or the D-allulose 3-epimerase enzyme of said recombinant host cells having D-allulose 3- epimerase activity.
The present invention relates to microbial production of nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), and nicotinamide adenine dinucleotide (NAD) using a genetically modified bacterium.
The present invention relates to improved methods for producing jasmonates such as jasmonic acid and methyl jasmonate in filamentous fungi under shaking conditions, hence enabling scale-up manufacturing processes using conventional fermenters. Specifically, one or more fungal quorum sensing molecules and/or jasmonate production elicitors can be added in the nutrient medium during cultivation of the filamentous fungi to induce formation of favorable morphologies and jasmonate production.
C07C 59/00 - Compounds having carboxyl groups bound to acyclic carbon atoms and containing any of the groups OH, O-metal, —CHO, keto, ether, groups, groups, or groups
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
The present invention relates, at least in part, to compounds and compositions that can be used to mask, block, or reduce the bitter taste present in various orally consumable products. The present invention also relates to methods of using bitterness blocking compounds and compositions to mask the bitterness of various orally consumable products, hence making such orally consumable products more palatable. The present invention further relates to an orally consumable product with reduced bitterness.
A23L 27/10 - Natural spices, flavouring agents or condimentsExtracts thereof
A61K 9/00 - Medicinal preparations characterised by special physical form
C07C 233/18 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
C07C 233/20 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton
Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
C12N 15/52 - Genes encoding for enzymes or proenzymes
C12P 23/00 - Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
Provided herein are recombinant nucleic acid molecules, nucleic acid constructs, fusion enzymes, transformed host cells, and methods for making aroma compounds alpha-ionone or beta-ionone.
The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I.
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The invention provides recombinant organisms containing a nucleic acid encoding a heterologous glycomolecule that has a low sulfation profile or that is unsulfated. In one embodiment the heterologous glycomolecule is an immunoglobulin molecule. The recombinant organisms have a genetic modification to at least one sulfotransferase gene, such as a deletion, disruption, or other genetic modification. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a mammalian cell, and therefore may be safer and more effective for use as a therapeutic in humans or animals. The glycomolecules can be a glycoprotein, glycopeptide, or a glycolipid.
Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12P 19/46 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
84.
Non-caloric sweeteners and methods for synthesizing
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
A23L 33/10 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof using additives
C12P 19/18 - Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
85.
Regulatory elements from labyrinthulomycetes microorganisms
The present disclosure generally relates to novel polynucleotide molecules for use in regulating gene expression in recombinant cells, such as labyrinthulomycetes cells. The disclosure further relates to nucleic acid constructs, such as vectors and expression cassettes, containing a regulatory element operably linked to a heterologous nucleotide sequence. The disclosure further relates to methods for stably transforming a host cell, such as a labyrinthulomycetes cell with transgenes. Stably transformed recombinant cells, progeny, biomaterials derived therefrom, and methods for preparing and using the same are also provided.
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
C12N 15/79 - Vectors or expression systems specially adapted for eukaryotic hosts
C12N 15/80 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
The present disclosure relates to the biosynthesis of UDP-Rhamnose and recombinant polypeptides having enzymatic activity useful in the relevant biosynthetic pathways for producing UDP-Rhamnose. The present invention also provides a method for preparing a steviol glycoside composition comprising at least one rhamnose-containing steviol glycoside.
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Biologics, namely, antibodies, and antigens for in vitro
scientific or research use, all produced by engineered
microbial cells, microbes and/or microbial systems;
microbial cells, microbes, and microbial systems for use in
the manufacture of biologics including antibodies, antigens,
and vaccines. Biologics, namely, antibodies, antigens, and vaccines all
for medical purposes and produced by engineered microbial
cells, microbes and/or microbial systems. Custom manufacturing of biologics including antibodies,
antigens, and vaccines using microbial cells, microbes,
and/or microbial systems. Research and development services in the field of biologics
including antibodies, antigens, and vaccines using microbial
cells, microbes, and/or microbial systems.
88.
BIOSYNTHETIC PRODUCTION OF GAMMA- AND DELTA-LACTONES USING CYTOCHROME P450 ENZYMES HAVING SUBTERMINAL HYDROXYLASE ACTIVITY
Provided herein are methods for making lactones comprising incubating a cellular system expressing a heterologous cytochrome P450 (CYP450) protein with a medium comprising straight chain fatty acids to produce subterminal hydroxy fatty acids, and incubating a yeast cell culture with the subterminal hydroxy fatty acids to produce lactones.
C08G 63/06 - Polyesters derived from hydroxy carboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from hydroxy carboxylic acids
The present disclosure relates to the use of beta-glucosidase to enhance the production efficiency of desired steviol glycosides, such as rebaudioside M (reb M).
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
90.
BIOSYNTHETIC PRODUCTION OF VARIANT STEVIOL GLYCOSIDES
The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.
C07H 15/24 - Condensed ring systems having three or more rings
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
91.
BIOSYNTHETIC PRODUCTION OF VARIANT STEVIOL GLYCOSIDES
The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C07H 15/24 - Condensed ring systems having three or more rings
The present invention relates, in some aspects, to the production of steviol glycoside rebaudioside D4 through the use of rebaudioside E. In some aspects, the invention relates to mutant CP1 enzymes, mutant HV1 enzymes as well as host cells and methods utilizing such enzymes, such as to produce rebaudioside D4.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
The present invention relates to the creation of a control system within a host cell to limit or eliminate degradation of key specified products at certain times during fermentation and to redirect the metabolic flux of the cell toward higher production of same key specified products.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 1/15 - Fungi Culture media therefor modified by introduction of foreign genetic material
C12N 15/11 - DNA or RNA fragmentsModified forms thereof
95.
Biosynthetic production of steviol glycosides rebaudioside J and rebaudioside N
The present disclosure relates to the production of steviol glycosides rebaudioside J and rebaudioside N through the use of rebaudioside A as a substrate and a biosynthetic pathway involving various 1,2 RhaT-rhamnosyltransferases.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.
The present invention relates, at least in part, to the production of steviol glycoside rebaudioside I through the use of variant UGT enzymes having activity to transfer a glucosyl group from UDP-glucose to rebaudioside A to produce rebaudioside I.
C12P 19/56 - Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
C12P 7/40 - Preparation of oxygen-containing organic compounds containing a carboxyl group
C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
01 - Chemical and biological materials for industrial, scientific and agricultural use
Goods & Services
ingredients manufactured by biosynthesis for use in further manufacturing of food, beverage, flavors, and fragrances, namely, plant-based sweeteners, glycans, proteins, amino acids, lactones, fatty acids, capsaicinoids, terpenoids, phenolics, and flavonoids
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
custom manufacturing of natural food, beverage, flavor, and fragrance ingredients, including natural ingredients manufactured by biosynthesis research and development services in the field of natural food, beverage, flavor, and fragrance ingredients, including natural ingredients manufactured by biosynthesis
