Disclosed herein are methods of making an implant material, the method involving harvesting allograft cancellous bone containing bone marrow from a donor within 24 hours of death; incubating the harvested allograft cancellous bone in an ultra-low freezer within 144 hours of death for at least 5 days; and then selectively lysing cells in the cancellous bone. This results in cancellous bone that is enriched for cells resistant to lysing, such as mesenchymal cells as well as a lysate containing factors (e.g. growth factors) released from the lysed cells. This lystate can be administered to cortical bone, e.g. demineralized cortical bone, with hydrochloric acid (HCI) or glycolytic acid so that these factors attach to the demineralized bone.
Disclosed herein are methods of making an implant material, the method involving harvesting allograft cancellous bone containing bone marrow from a donor within 24 hours of death; incubating the harvested allograft cancellous bone in an ultra-low freezer within 144 hours of death for at least 5 days; and then selectively lysing cells in the cancellous bone. This results in cancellous bone that is enriched for cells resistant to lysing, such as mesenchymal cells as well as a lysate containing factors (e.g. growth factors) released from the lysed cells. This lystate can be administered to cortical bone, e.g. demineralized cortical bone, with hydrochloric acid (HCI) or glycolytic acid so that these factors attach to the demineralized bone.
Provided herein are soluble soft tissue protein compositions that can contain one or more soft-tissue bioactive factors, methods of making the soluble soft tissue protein compositions, and methods of using the soluble soft tissue protein compositions.
A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
A23J 1/02 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from meat
A23J 1/04 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from fish or other sea animals
A61L 27/44 - Composite materials, i.e. layered or containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
4.
INCREASING VIABILITY OF CELLS WITHIN A FROZEN CELLULAR IMPLANT
The cryopreservation of cells from human tissue has challenges from both logistical timing as well as maintenance of cell viability. Therefore, needed are methods to increase cell viability in cellular tissue implants that allow for greater flexibility in timing for processing the implant. Therefore, disclosed herein is a method for increasing viability of cells within a frozen cellular implant, that involves soaking the cellular implant in a first cryoprotectant solution for at least 1 minute, soaking the cellular implant in a second cryoprotectant solution for at least 1 minute, and freezing the cellular implant. Also disclosed is a method for sterilizing a cellular implant comprised of collagen while maintaining cell viability that involves providing a cellular implant, adding a cryoprotectant to the cellular implant, freezing and maintaining a frozen temperature, and irradiating the cellular implant with radiation.
Described herein are methods to increase growth factor binding when demineralizing bone. Also described herein are methods of enriching bound growth factors in a medical implant.
Described herein are methods to increase growth factor binding when demineralizing bone. Also described herein are methods of enriching bound growth factors in a medical implant.
A61L 27/12 - Phosphorus-containing materials, e.g. apatite
A61L 27/44 - Composite materials, i.e. layered or containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
9.
Soft tissue protein compositions, methods of making, and uses thereof
Provided herein are soluble soft tissue protein compositions that can contain one or more soft-tissue bioactive factors, methods of making the soluble soft tissue protein compositions, and methods of using the soluble soft tissue protein compositions.
A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof
A23J 1/02 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from meat
A23J 1/04 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from fish or other sea animals
A61L 27/44 - Composite materials, i.e. layered or containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
Disclosed are various bioactive grafts and/or biocompatible materials and methods of making the same. In one embodiment, bone material is harvested from a donor. The harvested bone material is exposed to a lysing agent, the lysing agent configured to release growth factors and bioactive materials from cellular material of the harvested bone material. The harvested bone material is then rinsed with a rinsing agent. The pH of the harvested bone material is substantially neutralized. In another embodiment, an orthopaedic implant includes an antibacterial polysaccharide. The implant may also include an osteostimulative agent.
Provided herein are soluble bioactive factor solutions, grafting scaffolds containing the bioactive factor solutions, and methods of making and using the same.
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
a house mark for veterinary and medical compositions derived from or containing compositions or compounds derived from organisms for use in the manufacture of medical and veterinary preparations, implants, and implantable devices and scaffolds for animal and human use to treat conditions of bones and soft tissue, all for use in medical, veterinary, and cosmetic procedures a house mark for veterinary pharmaceutical compositions for animal use to treat conditions of bones and soft tissue, derived from or containing compositions or compounds derived from organisms; medical pharmaceutical compositions for human use to treat conditions of bones and soft tissue, derived from or containing compositions or compounds from organisms [ ; veterinary containers and delivery devices for medical and clinical use sold filled with veterinary pharmaceutical preparations and compositions for animal use to treat conditions of bones and soft tissue, derived from or containing compositions or compounds derived from organisms; medical containers and delivery devices for medical and clinical use sold filled with medical pharmaceutical preparations and compositions for human use to treat conditions of bones and soft tissue, all for use in medical, veterinary, and cosmetic procedures ] [ a house mark for veterinary preparations sold as a component of implantable veterinary devices; veterinary apparatuses and units for dosage, measuring and monitoring, namely, empty preparation delivery devices and containers, sold together as a unit with veterinary preparations for treating conditions of bones and soft tissues; medical preparations sold as a component of implantable medical devices for use in treating bone and soft tissue conditions; apparatuses and units for dosage, measuring and monitoring, namely, empty preparation delivery devices and containers, sold together as a unit with medical preparations for treating conditions of bones and soft tissues, all for use in medical, veterinary, and cosmetic procedures ]
01 - Chemical and biological materials for industrial, scientific and agricultural use
05 - Pharmaceutical, veterinary and sanitary products
10 - Medical apparatus and instruments
Goods & Services
biomaterial compositions comprised primarily of proteins for use in the manufacture of implantable scaffolds and implants used in surgical procedures biomaterial compositions comprised primarily of proteins for veterinary and medical use with implantable scaffolds and implants used in surgical procedures biomaterial compositions sold as a component of implantable scaffolds and implants used in surgical procedures
Disclosed are various bioactive grafts and methods of making the same. In one embodiment, bone material is harvested from a donor. The harvested bone material is exposed to a lysing agent, the lysing agent configured to release growth factors and bioactive materials from cellular material of the harvested bone material. The harvested bone material is then rinsed with a rinsing agent. The pH of the harvested bone material can be substantially neutralized.
