Abstract

Nutrient-enriched food products and methods of producing nutrient-enriched food products using a bacterium associated with a host from a nutrient-deficient environment.

IPC Classes  ?

• A23C 9/12 - Fermented milk preparationsTreatment using microorganisms or enzymes
• A23C 9/13 - Fermented milk preparationsTreatment using microorganisms or enzymes using additives
• A23L 11/50 - Fermented pulses or legumesFermentation of pulses or legumes based on the addition of microorganisms
• A23L 13/40 - Meat productsMeat mealPreparation or treatment thereof containing additives
• A23L 17/00 - Food-from-the-sea productsFish productsFish mealFish-egg substitutesPreparation or treatment thereof
• A23L 19/20 - Products from fruits or vegetablesPreparation or treatment thereof by pickling, e.g. sauerkraut or pickles
• A23L 33/135 - Bacteria or derivatives thereof, e.g. probiotics
• C12N 1/20 - BacteriaCulture media therefor

Abstract

The natural product neurolenin D is isolated from Neurolaena lobata using continuous extraction, stirring over charcoal, flash column chromatography, and recrystallization. The secondary alcohol in this molecule is converted into a methane sulfonyl ester (mesylate) to generate a new molecule, neurolenin D mesylate. This previously unknown molecule has been tested for biological activity in assays for human toxicity, mutagenicity, and nematocidal potency. It is not toxic or mutagenic and demonstrates the ability to kill nematodes responsible for the neglected tropical disease lymphatic filariasis and is likely to have the same ability to kill other nematodes responsible for a wide range of diseases in humans, animals and plants. Additional esters of neurolenin D are claimed and procedures are outlined for their synthesis.

IPC Classes  ?

• C07D 307/93 - Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems condensed with a ring other than six-membered
• A61P 33/10 - Anthelmintics

Abstract

The natural product neurolenin D is isolated from Neurolaena lobata using continuous extraction, stirring over charcoal, flash column chromatography, and recrystallization. The secondary alcohol in this molecule is converted into a methane sulfonyl ester (mesylate) to generate a new molecule, neurolenin D mesylate. This previously unknown molecule has been tested for biological activity in assays for human toxicity, mutagenicity, and nematocidal potency. It is not toxic or mutagenic and demonstrates the ability to kill nematodes responsible for the neglected tropical disease lymphatic filariasis and is likely to have the same ability to kill other nematodes responsible for a wide range of diseases in humans, animals and plants. Additional esters of neurolenin D are claimed and procedures are outlined for their synthesis.

IPC Classes  ?

• C07D 307/46 - Doubly bound oxygen atoms, or two oxygen atoms singly bound to the same carbon atom
• A61P 33/00 - Antiparasitic agents
• A01N 63/12 - Nematodes

Abstract

Platforms, including devices, systems, kits, and methods, are provided for the differential detection of Dirofilaria immitis and Dirofilaria. repens in an animal host using parasite-specific DNA target capture techniques as well as polymerase chain reaction detection of those targets.

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

Dirofilaria immitisDirofilaria. repensDirofilaria. repens in an animal host using parasite-specific DNA target capture techniques as well as polymerase chain reaction detection of those targets.

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

Platforms, including devices, systems, kits, and methods, are provided for the differential detection of Dirofilaria immitis and Dirofilaria. repens in an animal host using parasite-specific DNA target capture techniques as well as polymerase chain reaction detection of those targets.

IPC Classes  ?

• C12Q 1/6893 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa

Abstract

The present invention relates to methods for obtaining a calibration curve for quantifying protein copy number in immunofluorescence-based super resolution microscopy, for quantifying protein copy number in immunofluorescence-based super resolution microscopyand for determining the percentage of oligomeric state of a protein in a sample imaged with super- resolution microscopy. The invention also relates to a computer program and to a kits and uses thereof in the methods of the invention.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids