Abstract

The present invention relates to a novel integrated process to extract and purify levulinic acid (LA) produced from the technical lignin waste (2-G residue) generated after second generation (2-G) ethanol production from biomass. In this process, liquid-liquid extraction was carried out using fusel oil as an organic extractant to yield a LA rich organic phase and an acidic aqueous phase (raffinate). In addition, selected amines improved the LA yields by acting synergistically. Further fractional distillation was carried out to purify LA. The acidic raffinate is recycled back to the reaction in multiple times, which reduce the use of additional catalyst and make the overall process cost effective. The process is very simple and suitable to remove any colour and humins (soluble tar), which would otherwise form a problem in subsequent process step, particularly in distillation. At optimized process conditions 95% LA is recovered with 98% purity.

IPC Classes  ?

• C08J 11/16 - Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with inorganic material
• C07C 51/44 - SeparationPurificationStabilisationUse of additives by change of the physical state, e.g. crystallisation by distillation
• C07C 51/47 - SeparationPurificationStabilisationUse of additives by solid-liquid treatmentSeparationPurificationStabilisationUse of additives by chemisorption
• C07C 51/48 - SeparationPurificationStabilisationUse of additives by liquid-liquid treatment
• C07C 59/185 - Saturated compounds having only one carboxyl group and containing keto groups
• C08J 11/10 - Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
• C12P 7/22 - Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic

Abstract

The present invention relates to a process for production of xylitol. More particularly, the present invention provides an improved process for high titer xylitol production from pure xylose. The process of the present invention results in enhanced xylitol production in shortest duration of time.

IPC Classes  ?

• C12P 7/18 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric

Abstract

The present disclosure relates to a process for producing levulinic acid from the technical lignin. The technical lignin is rich in lignin and ash and do not have any other use and usually incinerated as a waste management strategy. During the incineration process, the cellulose also gets burnt and wasted. In the present disclosure, there is provided an economical and environmentally friendly process to produce levulinic acid from the technical lignin by using a synergistic combination of catalysts for high yields and high titer with acid recycling in a cost-effective manner

IPC Classes  ?

• C07C 51/487 - SeparationPurificationStabilisationUse of additives by treatment giving rise to chemical modification

Abstract

The present invention relates to a method for in-situ synthesis of alkyl esters of fatty acids and their efficient extraction from oleaginous microbes. Particularly, the present invention provides a rapid method for downstream processing of oleaginous biomass for in-situ synthesis of alkyl esters of fatty acids and their efficient extraction. The method of the present invention results in increased transesterification efficiency (>95%) of FAAE production from wet biomass, while eliminating the necessity of biomass harvesting, drying and large requirement of chemical solvents.

IPC Classes  ?

• C12P 7/6436 - Fatty acid esters
• C07C 67/56 - SeparationPurificationStabilisationUse of additives by solid-liquid treatmentSeparationPurificationStabilisationUse of additives by chemisorption
• C12N 1/12 - Unicellular algaeCulture media therefor

Abstract

Schizochytrium sp. (MTCC 5980) for use in continuous aerobic fermentative lipid production process for enhancing high value lipids like Docosahexaenoic acid (DHA), Docasapentaenoic acid (DPA), Eicosapentaenic acid (EPA) and lipids for biodiesel.

IPC Classes  ?

• C11B 5/00 - Preserving by using additives, e.g. anti-oxidants
• C11B 1/10 - Production of fats or fatty oils from raw materials by extracting

Abstract

Schizochytrium MTCC 5890 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

IPC Classes  ?

• C12N 1/12 - Unicellular algaeCulture media therefor
• C12R 1/89 - Algae

Abstract

The present invention provides an improved process for high titer lactic acid production using simultaneous saccharification and co-fermentation process using acid treated lignocellulosic biomass. The process comprises of pretreating lignocellulosic biomass comprising C5 and C6 sugars with dilute acid and superheated steam at a temperature ranging from 150-210° C.; neutralizing the pretreated lignocellulosic biomass of step (i) with NaOH pellets; and adding cellulase enzyme in the range of 1.5 to 10 FPU/g, bacteria, nutrients and buffering agent to the neutralized slurry obtained in step (ii) for hydrolysis and co-fermentation of C5 and C6 sugars at a temperature ranging from 40-45° C. for a period of 120-144 hours to obtain lactic acid. The process of the present invention requires simple nutrients and results in high titer lactic acid production within shortest duration of time.

IPC Classes  ?

• C12P 7/56 - Lactic acid
• C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase

Abstract

A dental implant assembly comprises, a base member which is rotatably insertable into a jaw-bone. The base member may be defined by a collar portion, a mid-portion and an apical region. The base member in entirety may be configured with a tapered configuration right from the mid-portion to the apical region. Further, the base member is defined with a plurality of threaded profiles on an outer surface. A first thread profile is defined on the collar portion. A second thread profile, defined on the mid-portion and a third thread profile, defined on the apical region. The third thread profile is configured to cut into the jaw-bone during insertion to anchor the base member. As the third thread profile cuts in to the jaw bone, the second thread profile forms a threaded hole within the drilled hole in order to securely anchor the base member within the jaw bone.

IPC Classes  ?

• A61C 8/00 - Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereonDental implantsImplanting tools

Abstract

An integrated approach for utilizing waste products of 2G bio-refineries to fractionate the lignin of high purity. The present invention also provides a method of recycling of two waste products (2-G ethanol residue as the substrate (LRBR) and fusel oil/synthetic fusel oil (SFO) as one of the solvent) of the biorefineries in a beneficial manner to fractionate the lignin of high purity. The present method of separating high purity lignin comprises fractioning extractive-free lignin rich residue with a solvent, wherein the solvent is a mixture of SFO/Fusel oil and Formic acid. An optimized ratio of waste fusel oil and formic acid gave more than >85% yields of high purity lignin.

IPC Classes  ?

• C07G 1/00 - Low-molecular-weight derivatives of lignin
• B01D 3/14 - Fractional distillation
• B01D 11/02 - Solvent extraction of solids
• B01D 11/04 - Solvent extraction of solutions which are liquid

Abstract

S. cerevisiae DBT-IOC S24 (MTCC25086) natural strain has been induced to produce xylitol from xylose at defined process parameters using xylose containing diluted acid pretreated biomass as well as from xylose containing synthetic media in presence of glucose, lignocelluloses biomass derived xylitol inducer maintaining aeration at 1VVM and pH at 5.0 in the medium.

IPC Classes  ?

• C12P 7/18 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
• C12P 7/14 - Multiple stages of fermentationMultiple types of microorganisms or reuse for microorganisms
• C12R 1/865 - Saccharomyces cerevisiae

Abstract

The present invention relates to a composite extract of Cocculus hirsutus for use in the prevention and treatment of dengue and its pharmaceutical compositions. Further, it provides for a stable pharmaceutical composition comprising a therapeutically effective amount of the said extract for use in the treatment of dengue virus infection in a mammal. It also provides for a method for reducing viral load in the treatment of dengue virus infection by administering the composite extract or its pharmaceutical composition to a mammal in need thereof.

IPC Classes  ?

• A61K 36/59 - Menispermaceae (Moonseed family), e.g. hyperbaena or coralbead
• A61K 31/473 - QuinolinesIsoquinolines ortho- or peri-condensed with carbocyclic ring systems, e.g. acridines, phenanthridines
• A61K 9/20 - Pills, lozenges or tablets
• A61P 31/14 - Antivirals for RNA viruses

Abstract

The present invention relates to a process for continuous production of second-generation ethanol from lignocellulosic biomass via continuous simultaneous saccharification and co-fermentation (SSCF) process, wherein the process includes a first fermentor vessel for selectively fermenting C5 sugars and then Continuous transferring the fermented biomass to a second fermentor vessel for hydrolyzing the fermented biomass and then Continuous transferring the hydrolysate to a third fermentor vessel for selectively fermenting C6 sugars to obtain ethanol. Overall, the ethanol yield achieved was up to 70% for both C5 and C6 sugars from pretreated biomass; and C5 utilization exceeded 95% after SSCF.

IPC Classes  ?

• C12P 7/06 - Ethanol, i.e. non-beverage
• C12P 7/10 - Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
• C12P 7/14 - Multiple stages of fermentationMultiple types of microorganisms or reuse for microorganisms

Abstract

The present invention provides antibodies against Lipocalin-2 (LCN2) and uses of the antibodies. the invention represents an advancement in the field of monoclonal antibodies for targeting cells overexpressing Lipocalin-2 (LCN2). The monoclonal antibodies disclosed in this invention has the capability to target LCN2 and inhibit LCN2-mediated tumour progression in a number of pathological conditions, including various forms of cancer.

IPC Classes  ?

• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• A61K 39/00 - Medicinal preparations containing antigens or antibodies

Abstract

A multifunctional chemical agents comprising functional agents Fn1, Fn2 and linkers, for the linchpin directed (LDM), protein directed (PDPM) modifications of proteins, and Fn1 accelerated kinetic labeling by Fn2.

IPC Classes  ?

• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides
• C07K 14/805 - HaemoglobinsMyoglobins
• C07K 1/13 - Labelling of peptides
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 1/00 - General processes for the preparation of peptides
• C07K 1/02 - General processes for the preparation of peptides in solution

Abstract

The present invention relates to an improved method of second generation ethanol production from a lignocellulosic biomass. The process comprises subjecting a slurry of pre-treated lignocellulosic biomass comprising C5 and C6 sugars in a fermentor; preferentially fermenting mainly C5 sugars by incubating the pretreated lignocellulosic biomass of step (i) with first cellulase enzyme, co-fermenting microorganism, nutrient in the fermentor at 30-37° C. for a period of 16 to 24 hours; adding second cellulase enzyme to the fermentation broth of step (ii) and increasing the temperature to 45-55° C. for hydrolysis; allowing the fermentation broth of step (iii) to cool to a temperature of 35-38° C.; and preferentially fermenting C6 sugars by incubating the broth of step (iv) with a second dose of co-fermenting microorganism for a period of 6-8 hours to obtain ethanol. The process results in high ethanol productivity in shortest duration of time.

IPC Classes  ?

• C12P 7/14 - Multiple stages of fermentationMultiple types of microorganisms or reuse for microorganisms
• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase

Abstract

Penicillium funiculosum MRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

IPC Classes  ?

• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
• C12N 1/14 - Fungi Culture media therefor
• C12R 1/80 - Penicillium

Abstract

A dental implant assembly comprises, a base member which is rotatably insertable into a jaw-bone. The base member may be defined by a collar portion, a mid-portion and an apical region. The base member in entirety may be configured with a tapered configuration right from the mid-portion to the apical region. Further, the base member is defined with a plurality of threaded profiles on an outer surface. A first thread profile is defined on the collar portion. A second thread profile, defined on the mid-portion and a third thread profile, defined on the apical region. The third thread profile is configured to cut into the jaw-bone during insertion to anchor the base member. As the third thread profile cuts in to the jaw bone, the second thread profile forms a threaded hole within the drilled hole in order to securely anchor the base member within the jaw bone.

IPC Classes  ?

• A61C 8/00 - Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereonDental implantsImplanting tools
• A61C 13/01 - Bases

Abstract

Penicillium funiculosum MRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

IPC Classes  ?

• C12N 1/14 - Fungi Culture media therefor
• C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
• C12R 1/80 - Penicillium

Abstract

Cocculus hirsutusCocculus hirsutus for use in the prevention and treatment of dengue and its pharmaceutical compositions. Further, it provides for a stable pharmaceutical composition comprising a therapeutically effective amount of the said extract for use in the treatment of dengue virus infection in a mammal. It also provides for a method for reducing viral load in the treatment of dengue virus infection by administering the composite extract or its pharmaceutical composition to a mammal in need thereof.

IPC Classes  ?

• A61K 36/18 - Magnoliophyta (angiosperms)
• A61P 31/12 - Antivirals

Abstract

Penicillium funiculosum MRJ-16 using minimum media and/or low cost media components.

IPC Classes  ?

• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
• C12N 1/14 - Fungi Culture media therefor
• C12P 21/00 - Preparation of peptides or proteins
• C12R 1/80 - Penicillium

Abstract

Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

IPC Classes  ?

• C12N 1/14 - Fungi Culture media therefor
• C12N 15/01 - Preparation of mutants without inserting foreign genetic material thereinScreening processes therefor
• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
• C12P 19/02 - Monosaccharides
• C12P 19/12 - Disaccharides
• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
• C12R 1/80 - Penicillium

Abstract

An advantageous process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass with reduced amount of inhibitors of catalysis comprising steps of: providing a feedstock comprising cellulosic biomass; chopping of cellulosic biomass to have a cellulosic biomass feed material of uniform size; feeding of uniform sized feed material to a horizontal counter current extraction unit; removing excess of water from the feed material obtained from the extraction unit; soaking the extruded feed material obtained from the extraction unit; optionally removing excess of water from the feed material obtained after acid soaking; steam pre-treating the feed material obtained after removing excess of water from the acid soaked feed material; neutralizing the slurry obtained from the steam pre-treating; and optionally saccharifying the neutralized slurry.

IPC Classes  ?

• C13K 1/02 - GlucoseGlucose-containing syrups obtained by saccharification of cellulosic materials
• C12P 19/02 - Monosaccharides
• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
• D21B 1/06 - Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres by dry methods
• D21B 1/36 - Explosive disintegration by sudden pressure reduction
• D21C 1/04 - Pretreatment of the finely-divided materials before digesting with acid reacting compounds

Abstract

The present invention relates to a formulation to stabilize lignocellulolytic enzyme mixture. This invention in particular relates to a formulation composed of lignocellulolytic enzyme with critical dosages of molasses either with petrochemical waste or glycerol as an additive to improve enzyme stability in a synergistic manner.

IPC Classes  ?

• C12N 9/96 - Stabilising an enzyme by forming an adduct or a compositionForming enzyme conjugates
• C12N 9/24 - Hydrolases (3.) acting on glycosyl compounds (3.2)

Abstract

A multi-dimensional micro fluid focussing device. The device includes an apparatus for multi-dimensional micro fluid focussing. The device also includes an analyser for analysing the focused fluid. The apparatus includes a microchannel having an inlet defining a first region, a middle region and an outlet defining a second region. A first junction is formed at the intersection of the first region and the middle region. A second junction is formed at the intersection of the middle region and the second region. A first sheath positioned proximal to the first junction and a second sheath positioned at the second junction. The junctions formed, along with the positioning of the sheaths enable the multi-dimensional focusing of the fluid. The analyser includes a holder for removably retaining the apparatus. A microscope is positioned across the holder. A recording unit is coupled to the microscope.

IPC Classes  ?

• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 15/10 - Investigating individual particles

Abstract

The present invention relates to an additive or additive composition for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis. More particularly, the present invention discloses petrochemical waste as an additive or an additive composition containing one or more ethylene glycol oligomers and one or more metal ions which are useful for improved production of fermentable sugars from lignocellulosic biomass during enzymatic hydrolysis.

IPC Classes  ?

• C08H 8/00 - Macromolecular compounds derived from lignocellulosic materials
• C12P 19/02 - Monosaccharides
• C12P 19/12 - Disaccharides
• D21C 5/00 - Other processes for obtaining cellulose, e.g. cooking cotton linters
• D21B 1/16 - Disintegrating in mills in the presence of chemical agents
• D21C 3/04 - Pulping cellulose-containing materials with acids, acid salts, or acid anhydrides

Abstract

Brevibacillus laterosporus LAK 1210 which results in amino acid substitution of tyrosine (Y) residue with histidine (H) at positions 221 and 720. The modified chitinase exhibits both exochitinase and endochitinase activity. The recombinant, modified enzyme is thermoactive with a temperature optimum of 55-60° C. and high thermostability (Tm of 66.7° C.), functions in a broad pH range (pH 3.0-11.0) having a pH optimum of 9.0 and also exhibits improved solubility and enhanced efficacy for control of insects and phytopathogenic fungi. The invention further provides improved methods for large scale production of recombinant modified chitinase and rapid, cost-effective purification method by chitin-adsorption affinity chromatography using powdered crustacean shells.

IPC Classes  ?

• C12N 9/42 - Hydrolases (3.) acting on glycosyl compounds (3.2) acting on beta-1, 4-glucosidic bonds, e.g. cellulase
• A01N 63/50 - Isolated enzymesIsolated proteins

Abstract

A multi-dimensional micro fluid focussing device. The device includes an apparatus for multi-dimensional micro fluid focussing. The device also includes an analyser for analysing the focused fluid. The apparatus includes a microchannel having an inlet defining a first region, a middle region and an outlet defining a second region. A first junction is formed at the intersection of the first region and the middle region. A second junction is formed at the intersection of the middle region and the second region. A first sheath positioned proximal to the first junction and a second sheath positioned at the second junction. The junctions formed, along with the positioning of the sheaths enable the multi-dimensional focusing of the fluid. The analyser includes a holder for removably retaining the apparatus. A microscope is positioned across the holder. A recording unit is coupled to the microscope.

IPC Classes  ?

• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry

Abstract

A multifunctional chemical agents comprising functional agents Fn1, Fn2 and linkers, for the linchpin directed (LDM), protein directed (PDPM) modifications of proteins, and Fn1 accelerated kinetic labeling by Fn2.

IPC Classes  ?

• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides

Abstract

A multifunctional chemical agents comprising functional agents Fn1, Fn2 and linkers, for the linchpin directed (LDM), protein directed (PDPM) modifications of proteins, and Fn1 accelerated kinetic labeling by Fn2.

IPC Classes  ?

• C07K 1/107 - General processes for the preparation of peptides by chemical modification of precursor peptides

Abstract

Clostridium sp., MTCC 25082 for use in an anaerobic fermentative hydrogen production process for enhancing hydrogen production.

IPC Classes  ?

• C12P 3/00 - Preparation of elements or inorganic compounds except carbon dioxide
• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/145 - Clostridium

Abstract

The present invention relates to an extract of Cissampelos pareira, its pharmaceutical compositions, and its therapeutic use in the prevention and treatment of dengue. It also relates to processes for the preparation of these extracts.

IPC Classes  ?

• A61K 36/59 - Menispermaceae (Moonseed family), e.g. hyperbaena or coralbead
• A61K 31/14 - Quaternary ammonium compounds, e.g. edrophonium, choline
• A61K 31/573 - Compounds containing cyclopenta[a]hydrophenanthrene ring systemsDerivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone
• A61K 36/18 - Magnoliophyta (angiosperms)

Abstract

The present invention discloses a method for producing readily available and hydrolysable polysaccharide-enriched biomass from lignocellulosic biomass while quantitatively retaining carbohydrate and producing fewer amounts of inhibitors. The method includes treating lignocellulosic biomass with an aqueous solution at elevated temperatures in a sequential step using hydrazine hydrate and sodium sulphite. In accordance with the present invention, the biomass may be further treated with a saccharification enzyme consortium to produce fermentable sugars.

IPC Classes  ?

• C12P 19/14 - Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase, e.g. by alpha-amylase
• C12P 19/02 - Monosaccharides

Abstract

The invention discloses nanoparticles comprising compounds of calcium with anions such as phosphate, pyrophosphate, sulphate, silicate, carbonate, molybdate, or phosphosilicate that are doped with various ions. The nanoparticles are configured to produce heat (hyperthermia) under radio-wave (1KHz-1000GHz) exposure together with magnetism suitable for contrast imaging in MRI, X-ray absorption for computed tomography, near-infrared optical fluorescence for optical imaging, and/or radio-isotope emission for nuclear imaging or therapy. The nanoparticles can also be incorporated into micro-beads or other 3 dimensional scaffolds for image-guided (MRI, CT, NIR, nuclear) tissue regeneration, vascular or tumor embolization, and/or chemo/radio-embolization.

IPC Classes  ?

• A61B 5/055 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fieldsMeasuring using microwaves or radio waves involving electronic [EMR] or nuclear [NMR] magnetic resonance, e.g. magnetic resonance imaging
• A61B 18/18 - Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by applying electromagnetic radiation, e.g. microwaves
• A61K 47/04 - Non-metalsCompounds thereof
• A61K 49/08 - Nuclear magnetic resonance [NMR] contrast preparationsMagnetic resonance imaging [MRI] contrast preparations characterised by the carrier

Abstract

The present invention relates to the continuous process of sequestration of nutrients from waste effluents released from gas fermentation plants or green-house gases emission managing plants by novel Thraustochytrids. Particularly this invention relates to the methods and systems to enhance sequestration rate and productivity of the process. This invention also relates to rapid biotransformation of nutrients present in waste effluents into high value omega-3 fatty acids like Docosahexaenoic acid (DHA), Docosapentaenoic acid (DPA), Eicosapentaenoic acid (EPA) and lipids for biodiesel. This disclosure is about means of processing of waste streams and producing value added products out of it.

IPC Classes  ?

• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12R 1/90 - Protozoa
• C02F 3/28 - Anaerobic digestion processes
• C02F 3/34 - Biological treatment of water, waste water, or sewage characterised by the microorganisms used
• C12N 1/10 - ProtozoaCulture media therefor
• C12N 1/12 - Unicellular algaeCulture media therefor
• C02F 101/10 - Inorganic compounds
• C02F 103/06 - Contaminated groundwater or leachate
• C02F 103/18 - Nature of the water, waste water, sewage or sludge to be treated from the wet purification of gaseous effluents

Abstract

The present invention provides a process for enzyme mediated hydrolysis of biomass for production of soluble sugars, wherein the said process comprises of steady addition of small portions of biomass to enzyme solution, enabling rapid solubilization of biomass. The process used for enzymatic saccharification allows for increased biomass loading, enzyme recycle and mitigation of substrate and product inhibitory effect. The recycling of unhydrolysed biomass along with soluble enzyme ensures complete reuse of the said enzyme for effective repeated hydrolysis thereby increasing the overall productivity of enzyme used.

IPC Classes  ?

• C12P 19/02 - Monosaccharides
• C12P 19/12 - Disaccharides

Abstract

The present invention relates to cell specific therapeutic modality by using a region of the PLAP Promoter. The invention further relates to specific expression of therapeutically PLAP useful sequences for specific transcriptional activation of this gene. The invention also relates to the PLAP region which may be used alone or in combination with other regions like enhancer sequences that augment cell or tumour specific gene expression.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression

Abstract

The invention relates to the production of rifamycin analogs against drug resistant bacteria. The invention also relates to antibacterial compounds against drug resistant mycobacteria. The invention also provides for pharmaceutical composition comprising antibacterial compound against drug resistant bacteria, particularly mycobacteria. The invention also provides for use of antibacterial compounds for the treatment of disease caused by bacteria and in particular mycobacteria.

IPC Classes  ?

• A61K 31/395 - Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
• A61K 31/4162 - 1,2-Diazoles condensed with heterocyclic ring systems
• C07D 498/00 - Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/01 - Bacteria or actinomycetales
• A61P 31/04 - Antibacterial agents
• A61P 31/06 - Antibacterial agents for tuberculosis

Abstract

The present invention relates to the field of double-stranded RNA (ds RNA) mediated gene silencing of root knot nematodes. The invention particularly provides an effective method for reducing the number of reproducing population and the number of progenies per individual of the root knot nematodes. The present invention also relates to host delivered dsRNA for controlling infection of root knot nematodes.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

The invention relates to a process for preparation of non-viral vector for delivery of nucleic acids by mucosal route comprising the steps' of (a) synthesis of pDNA loaded calcium phosphate nanoparticles using reverse micro-emulsion method having n- Hexane as oil phase and water as the aqueous phase; (b) addition of excess water to make total volume of water to adjust the molar ratio of water to AOT at 10 before stirriung both the micro-emulsions; (c) taking 1.36M of anhydrous calcium chloride and 0.35 M di-sodium hydrogen phosphate in two separate micro-emulsion system as the precursors; (d) adding 3μg of pDNA of interested into each system followed by continuous stirring for 12 hours; (e) mixing the micro- emulsion with di-sodium hydrogen phosphate to the micro-emulsion with calcium chloride at slow rate with continuous stirring at 4°C; (f) keeping the mixture, as obtained in step (e), undisturbed at low temperature under continuous stirring for 24 hours; (g) removing n- Hexane by using Bucci-evaporator and dissolving the resulting mass of AOT in 10 ml of absolute ethanol (99.9%) by vortexing; (h) centrifuging the solution for half an hour at 800 rpm at 4°C in a cold centrifuge; (i) washing the pelleted nanoparticles with absolute alcohol three times; (j) dispersing the pelleted nanoparticles in double distilled water at 4°C by vortexing to secure clear dispersion; and (k) dialyzing the dispersion, as obtained in step (j), in cold room using 14 kD dialysis membrane bag and followed finally for coating over nanoparticles with the polymers.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Solid lipid nanoparticles and the process thereof are disclosed. The solid lipid nanoparticles comprise: at least one lipid selected from the group consisting of glycerides and fatty acids, at least one hydrophilic drug or amphiphilic drug and at least one emulsifier.

IPC Classes  ?

• A61K 9/127 - Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant

Abstract

This invention relates to a novel non-obturation, regenerative technique "SealBio" of endodontic treatment involving regenerative potential of stem cells and signaling molecules, locally available in the peri - radicular region of teeth, wherein they are stimulated to induce healing and deposition of a natural barrier (seal) at the root apex.

IPC Classes  ?

• A61K 6/00 - Preparations for dentistry

Abstract

This invention relates to a method for detecting; (i) malignant astrocytoma from normal brain tissue (ii) glioblastoma and anaplastic astrocytoma (iii) primary glioblastoma and secondary glioblastoma (iv) progressive pathway and denovo pathway comprises determining the level of expression of miRNAs listed in table 2, 3, 4, 5, wherein a higher or lower level of expression of miRNAs in the test sample as compared to the control sample differentiates and kit for characterizing a) malignant astrocytoma from normal brain tissue cell comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing malignant astrocyoma from normal brain tissue cells b) glioblastoma from anaplastic astrocytoma comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing glioblastoma from anaplastic astrocyoma c) primary glioblastoma from secondary glioblastoma comprising reagent capable of specifically detecting the level of expression of the genes' of miRNAs and instructions for using said kit for characterizing primary glioblastoma from secondary glioldastoma, d) progressive pathway from denovo pathway comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing progressive pathway from denovo pathway.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

There is disclosed a vaccine and a pharmaceutical composition comprising hCG and Mycobacterium w suitable for administration to a subject in need thereof for the prevention and/or treatment of cancer. The compositions disclosed in the present invention when administered to a subject in need thereof results in enhanced immunogenicity and prevention against cancer. Further, the composition of the present invention when administered to a subject in need thereof results in inhibition of growth of tumor.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies

Abstract

A modified method of agglutination to detect infections caused by microorganisms comprising: staining the test serum, plasma or blood or purified antibodies with a protein stain; mixing serum, plasma or blood with stained antibodies with equal quantity of colored which antigen particles on a glass slide; adding diluted Antiglobulin conjugated with Biotin to the said mixture; subjecting the mixture to the step of mixing; adding diluted Avidin (preferably tagged with a visible indicator) to the mixture and thoroughly mixing all the ingredients.

IPC Classes  ?

• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

This invention relates to a high affinity recombinant humanized antibody fragment (scFv) specific for hepatitis B surface antigen having unique inter/intra chain bonding interaction because of 28 altered amino acid residues from the original mouse (5S) antibody and its chimeric Fab form, wherein fine tuning of the vernier zone residue makes it closer to the human sequence without any structural constraints.

IPC Classes  ?

• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C07K 16/08 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses

Abstract

A collector for fecal discharge is provided comprising a self-expanding resilient collection component, a housing sheath and a transit component. The collection component has an open proximal and distal end and a lumen connecting them. The component comprises interconnected resilient arms circumscribing its contour, wherein adjacent arms are resiliently biased away from each other for exerting outwardly radial pressure for expanding and anchoring the component to rectal walls upon deployment. The housing sheath comprises a flexible and resilient material overlaying at least one of inner or outer contour of collection component without interfering with or blocking its ends or lumen. The transit component provides a conduit for fecal discharge to migrate from collection component to a receptacle and comprises a flexible, tubular sheath having a first open end connected to a second end by a lumen, wherein the first end engages with the proximal end of the collection component.

IPC Classes  ?

• A61F 5/44 - Devices worn by the patient for reception of urine, faeces, catamenial or other dischargeColostomy devices

Abstract

The present invention relates to the anti dengue activity of the cissampelos pareira extracts. Pharmaceutical compositions comprising extracts of cissampelos pareira and processes for the preparation of extracts of cissampelos pareira are also provided.

IPC Classes  ?

• A61K 36/18 - Magnoliophyta (angiosperms)
• A61P 31/12 - Antivirals

Abstract

This invention relates to a Bicyclic triterpenoid Iripallidal as a novel anti- glioma and anti neoplastic agent in vitro, having the composition [(-) (6R, 1OS, 11S, 18R, 22S)-26 Hydroxy -22-α methylcyloirid- 16-enal NSC 631939], wherein it binds to Ras GRP3, a phorbol ester receptor that links DAG/phorbolester signaling with Ras activation and induces phosphorylation of ER Kl1/2 in a Ras dependent manner, reducing proliferation of cancer cell lines to the extent of 50-70%.

IPC Classes  ?

• C07C 403/00 - Derivatives of cyclohexane or of a cyclohexene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene rings, e.g. vitamin A, beta-carotene, beta-ionone
• A61K 31/122 - Ketones having the oxygen atom directly attached to a ring, e.g. quinones, vitamin K1, anthralin

Abstract

This invention relates to a technique to enhance the clinical efficiency of radiotherapy and readiosurgery using perturbative beaming and tissue specific radiobiology, comprising, Considering the normal steady beam dose rate, then increasing the temporal stochastic fluctuation of the output intensity of the radiation beam from a medical linear accelerator or cobalt gamma knife beam setup, developing a computer-based methodology by considering the tumor specific radiobiology and magnitude of the tailor made dose rate fluctuation using an embedded system device consisting of requisite hardware and software as described herein.

IPC Classes  ?

• A61N 5/10 - X-ray therapyGamma-ray therapyParticle-irradiation therapy

Abstract

The invention relates to a fully automated screening, diagnosis and classification technique for Alzheimer's disease using magnetic resonance imaging signals from the ventricular zone contour of the brain to get a fundamental index of brain deterioration comprising the steps: - obtaining a gray scale MRI image of the brain region; - applying a contour edge-detecting algorithm to the image; - employing a grid covering method for calculating a first order metric index of ventricular zone contour; - superimposing metric square grids of increasing edge length to the binary contour image and counting the metric grid squares; - plotting the logs of metric grid squares against the logs of edge lengths, wherein the gradient of the plot is the linear topological metric index.

IPC Classes  ?

• A61B 5/055 - Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fieldsMeasuring using microwaves or radio waves involving electronic [EMR] or nuclear [NMR] magnetic resonance, e.g. magnetic resonance imaging
• G06T 5/00 - Image enhancement or restoration

Abstract

A novel chemical moiety triterpenoid AECHL-1 isolated from root bark of Ailanthus excelsa having anti-cancer properties exhibiting remarkable activity in treatment of various forms of tumors with specificity wherein it blocks the growth of four tumor cell lines distinct origins and of different p53 status (B16F10 mouse melanoma, PC3 human prostate cancer, MCF-7 and MDA-MB-231 human breast cancer).

IPC Classes  ?

• C07D 493/04 - Ortho-condensed systems

Abstract

This invention relates to a novel recombinant chimera of DNA construct having esat-6 region of Mycobacterium tuberculosis and kinesin region of Leishmania donovani cloned together on two sides of self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the Chimeric construct is operatively linked to a transcriptional promoter thus capable of self replication and expression within the mammalian cell and the process of preparation thereof comprising. Analysis of the predicted protein sequence of kinesin motor domain and esat-6 domain using Promiscuous MHC Class-1 Binding Peptide Prediction Servers. Amplification of gene coding for kinesin motor domain and esat-6 domain. Cloning of kinesin esat-6 gene region in pGEM-T® vector for sequence analysis. Generation of Chimeric construct by directional cloning in pVAX-1 vector. In-vitro expression analysis of kenesin motor domain and esat-6 domain from the clones using cell free translation system and immunogenicity studies, Splenocyte proliferative and cytokines studies using the above mentioned constructs.

IPC Classes  ?

• A61K 39/04 - Mycobacterium, e.g. Mycobacterium tuberculosis
• A61K 39/008 - Leishmania antigens
• C12N 15/31 - Genes encoding microbial proteins, e.g. enterotoxins
• C12N 15/30 - Genes encoding protozoal proteins, e.g. from Plasmodium, Trypanosoma, Eimeria

Abstract

A method for medical image enhancement based on image transform resonance A novel method for Stochastic Resonance of medical image enhancement device based on the Integral Transform of the image, comprising: generating specific types of integral transforms like radon transform for CT, Fourier transform for MRI with image transform component; preparing stochastic perturbation waveform,with perturbator component performing the stochastic resonance on the image transform, with stochastic resonator component; characterizing the image enhancement factor of the SR-processed image with performance monitoring component; adjusting and controlling the bistability parameters of the double-well system that induces stochastic resonance with control component; arranging the provisional display of the array matrix of the SR-enhanced images, as the bistability parameters are varied with matrix display component; subjecting the final image to the step of display with final image display component.

IPC Classes  ?

• G06T 5/00 - Image enhancement or restoration
• G06T 5/40 - Image enhancement or restoration using histogram techniques

Abstract

The present invention provides a semi-synthetic sesquiterpene lactone parthenin compound of general formula 1, useful for cytotoxicity against cancer cell lines and anticancer activity. According to another aspect of the invention provides a pharmaceutical composition composing the said compound. The present invention further provides a therapeutically effective dose of the said composition and a method of treating cancer in a subject by adjninisjering the effective amount of esquiterpene lactone parthenin compound or pharmaceutical composition thereof by oral, nasal, intra-peritoneal, or intravenous. Wherein the form in which the said compisition used is liquid, tablet, capsule, powder, gel or granules.

IPC Classes  ?

• C07D 307/93 - Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems condensed with a ring other than six-membered
• C07D 405/04 - Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring- member bond
• C07D 407/04 - Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group containing two hetero rings directly linked by a ring-member-to-ring- member bond
• A61K 31/365 - Lactones

Abstract

A method of preparing dialysed aqueous extract of fenugreek seeds comprising washing the fenugreek seeds is distilled water, sterilizing the said seeds, subjecting the sterilized seeds to the step of grinding to form powder, suspending the said powder in phosphate buffered saline (PBS), subjecting the said suspension to the step of filtration to obtain the filtrate, treating the filtrate with activated charcoal to obtain clear supernatant, subjecting the supernatant to the step of lyophilization and the powder thus obtained was dissolved in phosphate buffered saline (PBS), dialyzing the aqueous extract of fenugreek seeds to obtain dialysed fenugreek seed extract (FSE) which was aliquoted and stored.

IPC Classes  ?

• A61K 36/00 - Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
• A61K 36/48 - Fabaceae or Leguminosae (Pea or Legume family)CaesalpiniaceaeMimosaceaePapilionaceae

Abstract

This invention relates to a high affinity recombinant humanized antibody fragment (scFv) specific for hepatitis B surface antigen having unique inter/ intra chain bonding interaction because of 28 altered amino acid residues from the original mouse (5S) antibody and its chimeric Fab form, wherein fine tuning of the vernier zone residue makes it closer to the human sequence without any structural constraints.

IPC Classes  ?

• C07K 16/08 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses

Abstract

A method for specifically labeling outer and inner segments and pedicles of mammalian cone photoreceptors in vivo comprising Intravitreal injection of 2 μl for adult anesthestized mouse (C57BL/6 J), 10 μl for guinea pig (Duncan Hartley) of 20 μl for monkey (Macaca mulatta) of PNA conjugated with a fluorescent probe (Rhodamine or Fluorescein) at a concentration of 0.005%, 0.01%, 0.02%, 0.05% or 0.5% (only 0.5% for guinea pig and monkey) with a glass electrode with tip diameter of ~ 1 μm or an insulin syringe posterior to the limbus into the vitreous of the eye, Removing the eye under anesthesia at various time points (mouse: 0.5, 1, 2, 4 and 8 hours; 1, 2, 4, 7 and 10 days; 1 and 3 months; guinea pig and monkey: 30 minutes) after the PNA injection, Hemisecting and fixing the posterior eye-cup in 4% paraformaldehyde for 1 hour at 4°C, Flat mounting the retina on a glass slide or cryosectioning, and observing PNA labeling with a fluorescence microscope.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

The invention in general relates to a process for diminishing the antigenicity and calcification of derived vascular graft when intended to be used as a implant, for rendering such grafts substantially resistant to infection, and for rendering such grafts liable to be stored for a prolonged period of time before implantation.

IPC Classes  ?

• A61L 27/36 - Materials for prostheses or for coating prostheses containing ingredients of undetermined constitution or reaction products thereof

Abstract

This invention relates to a simple end point assay for detection of transient intracellular Ca2+ with broad applicability to many Ca2+channel proteins comprising, Generation of expression constructs for the fusion proteins having the Ca2+/calmodulin dependent protein kinase II (CaMKII) phosphorylation sites of NR2A or NR2B subunits of N-methyl-D-aspartate receptor (NMDAR) or the voltage gated potassium channel of Drosophila (Eag) or any protein sequence which binds to the T-site of CaMKII similar to NR2B, conjugated to mitochondrial localizing signal sequence, or mutants of these sequences as described herein. Generation of mammalian expression constructs of α-CaMKll as a chimera with green fluorescent protein (GFP-D-CaMKII) or its mutants as described herein. Site-Directed mutagenesis, Transfection, Ca2+ stimulation, imaging and quantification of the number of cells with Ca2+-dependent signal, wherein, NMDA receptor activity assay, TRPVT receptor activity assay, GluR4 receptor activity assay are performed to detect the activity Of Ca2+ channel proteins.

IPC Classes  ?

• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing

Abstract

This invention relates to a process for the production of organic formulation of bio pesticide containing Pseudomonas Fluorescens comprising preparation of Mother culture of P. fluorescens and inoculating in King's B broth, at 30 ± 1° C for 24-36 hours followed by liquid fermentation process in 5-10% pongamia cake aqueous extract, 5-10% neem cake aqueous extract, 0.3-0.5% sugarcane molasses and King's B broth followed by solid fermentation using sterile Pongamia deoiled cake, Neem deoiled cake, wheat Bran.

IPC Classes  ?

• A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
• C12N 1/20 - BacteriaCulture media therefor

Abstract

A process for affecting reductive conversion reactions in a reactor using the palladized bacterial cellulose immobilized on a support for reductive conversion reactions in a reactor.

IPC Classes  ?

• B01J 23/44 - Palladium
• C02F 1/70 - Treatment of water, waste water, or sewage by reduction

Abstract

Chimeric tumor suppressor activating peptides derived from matrix attachment region binding protein (MARBP) SMARI unique in their sequence comprising a arginine rich motif flanked by serine residues wherein from the stretch of four consecutive serine residues the first serine residue gets phosphorylated by the protein kinase C family of serine threonine kinases being indispensable for its functionality, the phosphorylation being directly correlated to the phosphorylation of p53 at serine 15 residue thereby stabilizing it, wherein the peptide activates p53 by modifying it post translationally which allow phosphorylation and translocation of p53 to the nucleus.

IPC Classes  ?

• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• C07K 14/16 - HIV-1
• C07K 19/00 - Hybrid peptides

Abstract

The present invention relates to a self mixing anaerobic digester (1) useful for the treatment of solid organic waste. The digester comprises: i) at least one bottom (11) and at least one upper (12) reaction chamber hydraulically connected through a central draft pipe (13); ii) a feed tank (3) connected to the inlet pipe (4) of the bottom reaction chamber; iii) a feed preparation system (2); iv) an automatic control valve (7); v) a discharge tank (10) to collect the digested slurry.

IPC Classes  ?

• C12M 1/107 - Apparatus for enzymology or microbiology with means for collecting fermentation gases, e.g. methane
• C02F 3/28 - Anaerobic digestion processes

Abstract

A process for the construction of plant transformation vector comprising: digesting a binary vector pCAMBIA1304 with EcoR! and Sal I; subjecting the digested binary vector to the step of end filling by Klenow; re-tigat'ng the said binary vector by known method; obtaining CaMV 35 8 promoter cassette with Ω enhancer from pSH9; incorporating the said Camv35S prometer cassette into the Hind III site of the said binary vector to obtain modified pCAMBIA 1304 vetor; subjecting pore DNA fragment (1206 bp) to the step of amplification using a pair of primers; introducing EcoRI restriction sites to both the primers; ligating the said amplified cDNA fragment to pGEM T-easy; extracting EcoRI digested porC cDNA fragment from cloned pGEM T-easy; inserting said 'EcoRI digested porC Cdna fragment into modified pCAMBIA 1304 plant transformation vector to produce recombinant plasmids construct (pCAMBIA 1304-AtporC).

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/53 - Oxidoreductases (1)
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

A method of preparing dialysed aqueous extract of fenugreek seeds comprising washing the fenugreek seeds is distilled water, sterilizing the said seeds, subjecting the sterilized seeds to the step of grinding to form powder, suspending the said powder in phosphate buffered saline (PBS), subjecting the said suspension to the step of filtration to obtain the filtrate, treating the filtrate with activated charcoal to obtain clear supernatant, subjecting the supernatant to the step of lyophilization and the powder thus obtained was dissolved in phosphate buffered saline (PBS), dialyzing the aqueous extract of fenugreek seeds to obtain dialysed fenugreek seed extract (FSE) which was aliquoted and stored.

IPC Classes  ?

• A61K 36/48 - Fabaceae or Leguminosae (Pea or Legume family)CaesalpiniaceaeMimosaceaePapilionaceae

Abstract

An equipment is provided to measure the gas permeability of a solid permeable material. The gas permeated through the material is collected in a solvent and the amount of gas permeated is estimated.

IPC Classes  ?

• G01N 15/08 - Investigating permeability, pore volume, or surface area of porous materials

Abstract

Maintenance of quiescent hematopoietic stem and progenitor cells (HSPC) in culture without the addition of exogenous growth factors is an important aspect in clinical hematology. A recent report described the ability of Flt3 receptor-interacting lectin (FRIL) in the maintenance of cord blood (CB) derived progenitors in vitro. Since FRIL is a mannose binding lectin we examined the effectiveness of four such lectins of well-characterized specificities on the preservation of HSPC of CB origin. The HSPC preservation activity of lectins was assessed by in vitro colony forming unit (CFU) and long-term culture initiating cell (LTC-IC) assays. It was found that all four lectins had a HSPC preservation activity at least up to 30 days in culture without addition of exogenous growth factors. The results indicate that use of such lectins may provide a cost effective method of HSPC maintenance for clinical use.

IPC Classes  ?

• C12N 1/08 - Reducing the nucleic acid content
• C12N 5/0789 - Stem cellsMultipotent progenitor cells

Abstract

The present invention provides a novel pharmaceutical composition consisting of a combination of three coumarinolignoids of formula 1, 2 and 3 isolated from the seeds of the plant Cleome viscosa along with a pharmaceutically acceptable carrier useful as a immunomodulator. The invention also describes the ability of the compounds to modulate humoral and cell mediated immune response. It further provides a process for the preparation of a novel pharmaceutical composition of the said three coumarinolignoids in an optimized ratio to modulate humoral and cell mediated immune response.

IPC Classes  ?

• A61K 31/37 - Coumarins, e.g. psoralen
• A61P 37/02 - Immunomodulators

Abstract

The present invention relates to a microsatellite DNA marker used for identifying disease resistant populations of Penaeus monodon. In particular the invention concerns a microsatellite DNA to identify disease resistant populations of Penaeus monodon comprising a highly statistically significant DNA band of 71 bp and 317 bp bands.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

A process for the preparation of an antimicrobial extract from leaves of the plant Callistemon rigidus by cold extract using organic solvents selected from methanol, ethanol, chloroform, dichlorom ethane, hexane, diethyl and ether, concentrating the filtrate in vacuo to obtain the crude extract.

IPC Classes  ?

• A61K 36/61 - Myrtaceae (Myrtle family), e.g. teatree or eucalyptus