Apparatus and processes to grow a biomaterial made of aerial mycelium by sensing and controlling airborne mist concentration, regardless of relative humidity. A growth matrix comprising a growth medium and a fungus, is grown under controlled environmental conditions to produce a mycelium product. To control growth conditions precisely and efficiently, airborne mist is electronically detected using one or more sensors, configured to measure airborne mist concentration visually, optically, chemically, electromagnetically, or by laser, ultrasonically, with radar, or other means. Electronic detection of airborne mist using one or more sensors generates a signal that is transmitted to a processor that can either maintain, increase, or decrease airborne mist concentration in a growth environment. The present invention provides processes of growing mycelium that are repeatable and resource efficient, while providing high quality and quantity mycelium-based products.
Vegan leather; synthetic leather; untanned synthetic leather and vegan leather made from mycelium; synthetic leather and vegan leather made from mycelium; biofabricated materials being substitutes for animal skins for use in the manufacture of synthetic leather and textiles.
4.
SYSTEMS AND METHODS FOR GENERATING MYCELIA GROWTH FROM SUBSTRATES
Methods are disclosed for generating aerial mycelium, such as mycological material comprising solely mycelia from depleted substrate or depleted and rejuvenated substrate. Alternatively, methods are disclosed for generating mycelia and mushrooms from depleted substrate or depleted and rejuvenated substrate. Alternative methods are disclosed for generating differentiated mycelium materials using depleted substrate or depleted and rejuvenated substrate. The mycelia products that are generated can be used in the food industry (e.g., as a meat analog) and in other industries, such as textiles, packaging, and others. The present invention provides systems and methods for generating mycelia that are repeatable and energy efficient, while providing consistently high quality and quantity mycelium-based products.
Apparatus and methods to harvest biopolymer material, such as a mycological material comprising mycelia. The mycelia products that are harvested can be used in the food industry (for example, as an animal-based meat-substitute) and in other industries, such as textiles, packaging, and others. The present invention provides mycelial harvesting methods and systems that are repeatable and energy efficient, while providing high quality and quantity mycelium-based products.
METHODS FOR CONTROLLING GEOMETRIC REGULARITY AND HOMOGENEITY OF AERIAL MYCELIUM TOPOLOGIES AND PRODUCTS OF AERIAL MYCELIUM WITH GEOMETRICALLY REGULAR OR HOMOGENEOUS TOPOLOGIES
This application relates to a method for growing aerial mycelium with a regular growth pattern and products of aerial mycelium biopolymers with regular growth patterns. For example, a regular growth pattern can include a homogeneous growth topology or a geometrically regular pattern of bulbous forms. As further examples, the method for affecting growth topology can include controlling environmental conditions or using a topology adjustment layer.
A01G 24/00 - Growth substratesCulture mediaApparatus or methods therefor
E04B 1/62 - Insulation or other protectionElements or use of specified material therefor
E04B 1/74 - Heat, sound or noise insulation, absorption, or reflectionOther building methods affording favourable thermal or acoustical conditions, e.g. accumulating of heat within walls
E04B 5/16 - Load-carrying floor structures wholly or partly cast or similarly formed in situ
E04B 5/43 - Floor structures of extraordinary designFeatures relating to the elastic stabilityFloor structures specially designed for resting on columns only, e.g. mushroom floors
E04C 2/16 - Building elements of relatively thin form for the construction of parts of buildings, e.g. sheet materials, slabs, or panels characterised by specified materials of wood, fibres, chips, vegetable stems, or the likeBuilding elements of relatively thin form for the construction of parts of buildings, e.g. sheet materials, slabs, or panels characterised by specified materials of plasticsBuilding elements of relatively thin form for the construction of parts of buildings, e.g. sheet materials, slabs, or panels characterised by specified materials of foamed products of fibres, chips, vegetable stems, or the like
D06N 3/00 - Artificial leather, oilcloth, or like material obtained by covering fibrous webs with macromolecular material, e.g. resins, rubber or derivatives thereof
This application relates generally to aerial mycelium and methods of making aerial mycelium suitable for use as a food or textile product or ingredient. Such a food product or ingredient can include edible aerial mycelium having a texture that is analogous to a whole-muscle meat product, such as for example mycelium-based bacon. Such a textile product or ingredient can be used in the manufacture of mycelium-based textile products, leather-like materials, petroleum-based product alternatives, or foams.
A01G 24/00 - Growth substratesCulture mediaApparatus or methods therefor
A23L 31/00 - Edible extracts or preparations of fungiPreparation or treatment thereof
D06N 3/00 - Artificial leather, oilcloth, or like material obtained by covering fibrous webs with macromolecular material, e.g. resins, rubber or derivatives thereof
8.
EDIBLE AERIAL MYCELIA AND METHODS OF MAKING THE SAME
An improved mycelium in the form of an edible aerial mycelium that is suitable for use as a food product, including a food ingredient for making mycelium-based food, such as bacon. A method of making an edible aerial mycelium suitable for use as a food product, including a food ingredient. An edible product containing an edible aerial mycelium, and a method of making an edible product comprising an edible aerial mycelium, such as a mycelium-based bacon. A mycelium-based food product having a texture that is analogous to a whole-muscle meat product, wherein that whole-muscle meat product is bacon.
This application relates generally to aerial mycelium and methods of making aerial mycelium suitable for use as a food or textile product or ingredient. Such a food product or ingredient can include edible aerial mycelium having a texture that is analogous to a whole-muscle meat product, such as for example mycelium-based bacon. Such a textile product or ingredient can be used in the manufacture of mycelium-based textile products, leather-like materials, petroleum-based product alternatives, or foams.
A01G 24/00 - Growth substratesCulture mediaApparatus or methods therefor
A23L 31/00 - Edible extracts or preparations of fungiPreparation or treatment thereof
D06N 3/00 - Artificial leather, oilcloth, or like material obtained by covering fibrous webs with macromolecular material, e.g. resins, rubber or derivatives thereof
10.
EDIBLE AERIAL MYCELIA AND METHODS OF MAKING THE SAME
An improved mycelium in the form of an edible aerial mycelium that is suitable for use as a food product, including a food ingredient for making mycelium-based food, such as bacon. A method of making an edible aerial mycelium suitable for use as a food product, including a food ingredient. An edible product containing an edible aerial mycelium, and a method of making an edible product comprising an edible aerial mycelium, such as a mycelium-based bacon. A mycelium-based food product having a texture that is analogous to a whole-muscle meat product, wherein that whole-muscle meat product is bacon.
01 - Chemical and biological materials for industrial, scientific and agricultural use
17 - Rubber and plastic; packing and insulating materials
18 - Leather and imitations of leather
31 - Agricultural products; live animals
37 - Construction and mining; installation and repair services
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Mycelium, namely, vegetative mushroom root structures, for use in manufacture and industry; mycelium, namely, vegetative mushroom root structures that are grown and processed into biofabricated solid materials for use in the manufacture of a wide variety of consumer products. Biofabricated foams and elastomers made from mycelium for use in the manufacture of a wide variety of consumer products. Untanned synthetic leather and vegan leather made from mycelium; synthetic leather and vegan leather made from mycelium; biofabricated materials; biofabricated materials made from mycelium being substitutes for animal skins for use in the manufacture of synthetic leather and textiles. Mycelium; kits for growing mycelium, namely, vegetative mushroom root structures, and processing mycelium to create biofabricated solid materials, namely, kits comprised of dormant mycelium material, a growing substrate and a growth form. Installation of growth systems for growing fungi for the commercial production of mycelium-based materials. Custom manufacturing of mycelium-based materials. Research and design in the fields of production and use of mycelium-based materials, growth systems and equipment for growing fungi for the commercial production of mycelium-based materials, and biofabrication; product research and development; product development and product development consulting in the field of mycelium-based materials; consultation services in the fields of production of mycelium-based materials and biofabrication; custom design of growth systems for growing fungi for the commercial production of mycelium-based materials.
17 - Rubber and plastic; packing and insulating materials
18 - Leather and imitations of leather
Goods & Services
Biofabricated foams and elastomers made from mycelium for use in the manufacture of a wide variety of consumer products. Untanned synthetic leather and vegan leather made from mycelium; synthetic leather and vegan leather made from mycelium; biofabricated materials made from mycelium being substitutes for animal skins for use in the manufacture of synthetic leather and textiles.
17 - Rubber and plastic; packing and insulating materials
18 - Leather and imitations of leather
Goods & Services
Biofabricated foams and elastomers made from mycelium for use in the manufacture of a wide variety of consumer products. Untanned synthetic leather and vegan leather made from mycelium; synthetic leather and vegan leather made from mycelium; biofabricated materials made from mycelium being substitutes for animal skins for use in the manufacture of synthetic leather and textiles.
01 - Chemical and biological materials for industrial, scientific and agricultural use
31 - Agricultural products; live animals
37 - Construction and mining; installation and repair services
40 - Treatment of materials; recycling, air and water treatment,
42 - Scientific, technological and industrial services, research and design
Goods & Services
Mycelium, namely, vegetative mushroom root structures, for use in manufacture and industry; mycelium, namely, vegetative mushroom roots structures that are grown and processed into biofabricated solid materials for use in the manufacture of a wide variety of consumer products. Mycelium; kits for growing mycelium, namely, vegetative mushroom root structures, and processing mycelium to create biofabricated solid materials, namely, kits comprised of dormant mycelium material, a growing substrate and a growth form. Installation of growth systems for growing fungi for the commercial production of mycelium-based materials. Custom manufacturing of mycelium-based materials. Research and design in the fields of production and use of mycelium-based materials, growth systems and equipment for growing fungi for the commercial production of mycelium-based materials, and biofabrication; product research and development; product development and product development consulting in the field of mycelium-based materials; consultation services in the fields of production of mycelium-based materials and biofabrication; custom design of growth systems for growing fungi for the commercial production of mycelium-based materials.
An improved mycelium in the form of an edible aerial mycelium that is suitable for use as a food product, including a food ingredient for making mycelium-based food, such as bacon. A method of making an edible aerial mycelium suitable for use as a food product, including a food ingredient. An edible product containing an edible aerial mycelium, and a method of making an edible product comprising an edible aerial mycelium, such as a mycelium-based bacon. A mycelium-based food product having a texture that is analogous to a whole-muscle meat product, wherein that whole-muscle meat product is bacon.
An improved mycelium in the form of an edible aerial mycelium that is suitable for use as a food product, including a food ingredient for making mycelium-based food, such as bacon. A method of making an edible aerial mycelium suitable for use as a food product, including a food ingredient. An edible product containing an edible aerial mycelium, and a method of making an edible product comprising an edible aerial mycelium, such as a mycelium-based bacon. A mycelium-based food product having a texture that is analogous to a whole-muscle meat product, wherein that whole-muscle meat product is bacon.
Topical applicators containing mycological biopolymers are suitable for applying health or cosmetic products to the skin or lips of a subject. The applicators are made from biodegradable mycological biopolymers that are grown from fungi in the presence of a growth medium under a predetermined environment of relative humidity, temperature, carbon dioxide, oxygen and air flow. The topical applicators containing the mycological biopolymers may further contain beneficial agents or products that that may be transferred to the skin or lips, thereby enhancing the health or beauty of a subject.
Several types of non-agricultural lignocellulosic waste media are disclosed for the growth of mycological biopolymers. The growth medium is comprised of a substrate with a composition of appropriate Carbon, Nitrogen and mineral components including but not limited to lipids, proteins, and other inherent nutrition requisite for mycelial growth. Specific examples are (1) a lignocellulosic material, (2) a mineral based material, (3) a non-toxic, organic or inorganic, non-lignocellulosic material, (4) a synthetically sourced and produced material, (5) a whole tree (flourized), and (6) an agar media.
C12N 1/22 - Processes using, or culture media containing, cellulose or hydrolysates thereof
C12N 11/14 - Enzymes or microbial cells immobilised on or in an inorganic carrier
A23K 10/37 - Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hayAnimal feeding-stuffs from material of fungal origin, e.g. mushrooms from waste material
Several methods are described for generating mycelial scaffolds for use several technologies. In one embodiment, a mycelial scaffold is generated using a perfusion bioreactor system for cell-based meat technologies. In another embodiment, a mycelial scaffold is prepared for biomedical applications. The mycelial scaffolds may be generated from a liquid medium or from a solid substrate.
The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.
C12M 1/04 - Apparatus for enzymology or microbiology with gas introduction means
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
21.
A BIOREACTOR PARADIGM FOR THE PRODUCTION OF SECONDARY EXTRA-PARTICLE HYPHAL MATRICES
The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.
A panel of mycological polymer consisting entirely of fungal mycelium as described in US Patent Application 16/190,585 is post-processed to impart desired characteristics thereto, such as, texture, flavor and nutritional profile for use as a foodstuff or a tissue scaffold. Alternatively, the growth conditions of the growth media may be tailored to obtain a desired density, morphology, and/or composition of the undifferentiated fungal material with or without the use of post-processes.
A23J 1/00 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites
A23J 3/20 - Proteins from microorganisms or unicellular algae
A23L 5/00 - Preparation or treatment of foods or foodstuffs, in generalFood or foodstuffs obtained therebyMaterials therefor
A23L 13/00 - Meat productsMeat mealPreparation or treatment thereof
A23L 31/00 - Edible extracts or preparations of fungiPreparation or treatment thereof
A23L 33/00 - Modifying nutritive qualities of foodsDietetic productsPreparation or treatment thereof
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
C12N 5/00 - Undifferentiated human, animal or plant cells, e.g. cell linesTissuesCultivation or maintenance thereofCulture media therefor
C12N 5/077 - Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
23.
METHOD OF PRODUCING A MYCOLOGICAL PRODUCT AND PRODUCT MADE THEREBY
A panel of mycological polymer consisting entirely of fungal mycelium as described in US Patent Application 16/190,585 is post-processed to impart desired characteristics thereto, such as, texture, flavor and nutritional profile for use as a foodstuff or a tissue scaffold. Alternatively, the growth conditions of the growth media may be tailored to obtain a desired density, morphology, and/or composition of the undifferentiated fungal material with or without the use of post-processes.
The mycelial foam contains macroscopic void spaces that are formed by filler elements, such as agar beads, that are incorporated in the mycelial matrix during growth of the matrix and are removed from the matrix after growth in a non-destructive manner, such as by heating. The foam may be made of pure mycelium or may be a composite biomaterial.
B29C 45/00 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor
B29C 45/14 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
B29C 69/00 - Combinations of shaping techniques not provided for in a single one of main groups , e.g. associations of moulding and joining techniquesApparatus therefor
25.
PROCESS AND APPARATUS FOR PRODUCING MYCELIUM BIOMATERIAL
The process for producing mycelium biomaterial provides fresh oxygen to the growing mycelium biomaterial while removing waste heat and waste carbon dioxide by forced aeration through large volumes of material. In a first phase of fungal expansion, humidified air at a programmed temperature is passed upwardly and through a fungal inoculated substrate of discrete particles to allow the fungal inoculum to expand and dominate the substrate. Nutrient is added to the inoculated mixture and a second phase of fungal expansion is performed wherein humidified air at a programmed temperature is passed upwardly and through the nutrient enriched fungal inoculated substrate to allow the fungal inoculum to bond the discrete particles into a self-supporting biocomposite. The process and apparatus of the invention allows for the processing of grown materials bound by mycelium at depths of greater than 6" and particularly in the range of from 24" to 28".
A61K 36/8962 - Allium, e.g. garden onion, leek, garlic or chives
A61P 25/28 - Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
26.
PROCESS FOR MAKING MINERALIZED MYCELIUM SCAFFOLDING AND PRODUCT MADE THEREBY
The process of making a mineralized mycelium scaffolding requires obtaining a scaffold of fungal biopolymer having a network of interconnected mycelia cells, functionalizing the biopolymer to create precursor sites and thereafter mineralizing the scaffold with one of silicates, apatites and carbonates. The mineralized mycelium scaffolding may be used for medical applications in place of mineralized collagen membranes and collagen/hydroxyapatite composite scaffolds.
The method of growing a biopolymer material employs incubation of a growth media comprised of nutritive substrate and a fungus in containers that are placed in a closed incubation chamber with air flows passed over each container while the chamber is maintained with a predetermined environment of humidity, temperature, carbon dioxide and oxygen. The air flows may be directed parallel or perpendicularly to the surfaces of the growth media.
The method of growing a biopolymer material employs incubation of a growth media comprised of nutritive substrate and a fungus in containers that are placed in a closed incubation chamber with air flows passed over each container while the chamber is maintained with a predetermined environment of humidity, temperature, carbon dioxide and oxygen. The air flows may be directed parallel or perpendicularly to the surfaces of the growth media.
A mycological biopolymer material is subjected to treatment in one or more solutions that work to enhance and/or retain the inherent material properties of the material. In one embodiment, the solution is an organic solution; in another embodiment, the solution is an organic solvent with a salt; in another embodiment, the solution is an organic solvent phenol and/or polyphenol; and in another embodiment, a series of such solutions is used.
A method of producing a mycological composite material comprises inoculating a substrate of fibrous material with an inoculum of mycelial tissue; rolling the inoculated substrate into a roll; and thereafter incubating the rolled inoculated substrate for a time sufficient for the mycelial tissue to grow hyphae that enmesh with the substrate to form a cohesive unified filamentous network with the rolled inoculated substrate being characterized in being flexible. The rolled inoculated substrate may be subsequently processed by subjecting lengths of the roll to heat and pressure in molds to form rigid products.
In one embodiment, the process combines a fraction (up to 15%) of a lignocellulose substrate with supplemental nutritional material and hydrates the fraction to a moisture content of from 40% to 70% by weight. The hydrated substrate fraction is heat processed to remove ambient bioburden (yeast, mold, bacteria) and to maintain the hydrated substrate fraction in an aseptic condition. Thereafter, the hydrated substrate fraction is inoculated with a fungus and incubated to obtain a myceliated substrate which is then reduced into discrete particles. The remaining fraction of the substrate is combined with water and then combined with the discrete particles of myceliated substrate and incubated to obtain a second myceliated substrate which is then reduced into discrete particles. The second myceliated substrate is combined with supplemental nutritional material and incubated to obtain a third myceliated substrate composed of at least 10% mycelium.
A method of producing an absorbent biocompatible material which comprises mixing at least one mycological material, such as a fungus or fungal tissue, with at least one lignocellulosic material to provide a biocomposite material comprising the at least one mycological material and the at least one lignocellulosic material. The biocomposite material then is contacted with at least one substance that increases the hydrophilicity of the biocomposite material, thereby providing an absorbent biocomposite material that has a variety of uses, such as absorbent materials for use in vegetation propagation and preservation, animal bedding, animal litter, filler in diapers, cold chain materials for temperature regulation, ice packs, absorbent foams and mats, such as decorative floral arrangement foams, carpet mats, and absorbent booms applied to chemical spills.
The machine and method employ a moving fluid, either a gas or liquid that is moved through a three-dimensional cavity of a form covered in part with a filter material. The filler material is introduced to the fluid stream and is deposited in the three-dimensional cavity of the form as the moving fluid is filtered from the stream by the filter material.
A living hydrated mycelium composite containing at least one of a combination of mycelium and fibers, mycelium and particles, and mycelium, particles and fibers is processed with a nutrient material to promote mycelia tissue growth; thereafter dehydrated to a moisture content of less than 50% by weight to deactivate the further growth of mycelia tissue; and then stored in the form of pellets. The stored may thereafter be re-hydrated and molded or cast into panels that can be separated into cubes or bricks that can be stacked and re-hydrated for making fabricated sections.
The process of growing a homogeneous polymer matrix comprising the steps of growing a viable mycelium in a liquid suspension; extracting mycelium from the liquid suspension; thereafter incubating the mycelium for a period of time sufficient to induce mycelium cohesion and to form a solid material; and thereafter drying the solid material to remove moisture and to inactivate the mycelium.
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
37.
METHOD FOR PRODUCING RAPIDLY RENEWABLE CHITINOUS MATERIAL USING FUNGAL FRUITING BODIES AND PRODUCTS MADE THEREBY
The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.
C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor
38.
METHOD FOR PRODUCING GROWN MATERIALS AND PRODUCTS MADE THEREBY
The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The composite material is made by inoculating a substrate of discrete particles and a nutrient material with a preselected fungus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material. In another embodiment, the fungus is allowed to grow as a fruiting body out of the substrate and within an enclosure to completely fill the enclosure to form a self-supporting structure.
C12P 1/02 - Preparation of compounds or compositions, not provided for in groups , by using microorganisms or enzymesGeneral processes for the preparation of compounds or compositions by using microorganisms or enzymes by using fungi
