The present invention relates to modified bacteria for introducing desired mutations in target sequences in plant cells, wherein the bacteria has reduced VirD5 activity and carries site-specific DNA editing machinery. The invention also provides methods for generating plant cells, plant parts, plants or populations thereof using such bacteria.
The present invention provides improved methods for generating banana embryos and plants carrying desired mutations in target sequences, comprising introducing at least one mutation in at least one endogenous acetolactate synthase (ALS) gene. The invention also provides banana plants and products generated by such methods.
The present invention relates to compositions and methods for the delay or prevention of browning in banana fruit by genetically editing one or more genes encoding a Polyphenol Oxidase (PPO).
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
5.
COMPOSITIONS AND METHODS FOR INCREASING EXTRACTIBILITY OF SOLIDS FROM COFFEE BEANS
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase. Also provided is a method of increasing extractability of solids from coffee beans. In addition there is provided a method of producing soluble coffec.
A banana plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding a component in an ethylene biosynthesis pathway of the banana is provided. Also provides is a method of increasing shelf-life of banana.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.
The present invention relates to modified Agrobacteria for introducing desired mutations in target sequences in plant cells, wherein the bacteria have reduced VirD5 expression and carry site-specific DNA editing machinery. The invention also provides methods for generating plant cells, plant parts, plants or populations thereof using such bacteria.
The present invention relates to modified Agrobacteria for introducing desired mutations in target sequences in plant cells, wherein the bacteria have reduced VirD5 expression and carry site-specific DNA editing machinery. The invention also provides methods for generating plant cells, plant parts, plants or populations thereof using such bacteria.
The present invention relates to compositions and methods for the delay or prevention of browning in banana fruit by genetically editing one or more genes encoding a Polyphenol Oxidase (PPO).
The present invention relates to compositions and methods for the delay or prevention of browning in banana fruit by genetically editing one or more genes encoding a Polyphenol Oxidase (PPO).
The present invention provides improved methods for generating banana embryos and plants carrying desired mutations in target sequences, comprising introducing at least one mutation in at least one endogenous acetolactate synthase (ALS) gene. The invention also provides banana plants and products generated by such methods.
A method of producing a long dsRNA molecule in a plant cell that is capable of silencing a pest gene is provided, the method comprising: (a) selecting m a genome of a plant a nucleic acid sequence encoding a silencing molecule having a plant gene as a target, the silencing molecule capable of recruiting RNA-dependent RNA Polymerase (RdRp); and (b) modifying a nucleic acid sequence of the plant gene so as to impart a silencing specificity towards the pest gene, such that a transcript of the plant gene comprising the silencing specificity forms base complementation with said silencing molecule capable of recruiting said RdRp to produce the long dsRNA molecule capable of silencing the pest gene, thereby producing the long dsRNA molecule m the plant cell that is capable of silencing the pest gene.
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Genetic engineering services relating to plants; plant engineering services; gene editing services relating to plants; gene editing services; agricultural research; genetic engineering services; scientific research in the fields of genetics, gene editing, gene expression, gene silencing and genetic engineering; testing, inspection and research services in the fields of agriculture and breeding of plants and animals; laboratory research in the field of gene expression; laboratory research in the field of gene editing; genetic testing for scientific research purposes; technological consultancy and research in the fields of genetics, gene editing, gene expression, gene silencing and genetic engineering; genetic engineering services in the fields of transgenic and gene-edited plant and animal generation, gene cloning, genetic transformation of crops, animals and plants, molecular assays, DNA-based assays, RNA-based assays, genotyping, growing plants in greenhouses and controlled plant-growing environments, biochemical analysis, enzymatic assays, protein analysis, transient cell based analysis, plant phenotyping and custom assay development; product development consultancy in the fields of molecular biology, genetics, gene-editing and molecular engineering; product development of genetically edited plants Licensing of intellectual property; licensing of software
42 - Scientific, technological and industrial services, research and design
45 - Legal and security services; personal services for individuals.
Goods & Services
Genetic engineering services relating to plants originating from arid, Mediterranean, temperate, continental and polar climates; plant engineering services relating to plants originating from arid, Mediterranean, continental and polar climates; gene editing services relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; provision of a gene editing platform for use in relation to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; agricultural research in relation to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; genetic engineering services relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; scientific research in the field of genetics, gene editing, gene expression, gene silencing and genetic engineering, all of the aforesaid relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; testing, inspection and research services in the fields of agriculture and breeding, all of the aforesaid relating to plants, human animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; laboratory research in the field of gene expression relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; laboratory research in the field of gene editing and molecular engineering relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; development of genetically edited plants originating from arid, Mediterranean, temperate, continental and polar climates; genetic testing of plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates for scientific research purposes; technological consultancy and research in the fields of plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; information technology engineering services in the fields of plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; product development consultancy in the fields of molecular biology, genetics, gene-editing and molecular engineering, all of the aforesaid relating to plants, humans, animals, amphibians, fish, shellfish, crustaceans, mammals, reptiles, birds and insects originating from arid, Mediterranean, temperate, continental and polar climates; development of genetically edited plants originating from arid, Mediterranean, temperate, continental and polar climates. Licensing of intellectual property; licensing of software; licensing of a gene editing platform.
19.
MODIFYING THE SPECIFICITY OF NON-CODING RNA MOLECULES FOR SILENCING GENES IN EUKARYOTIC CELLS
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, wherein the gene encoding or processed into the non-coding RNA molecule is positioned in a coding gene, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Target RNA of interest include, for example, a transcript of a gene selected from the group consisting of a housekeeping gene, a dominant gene, a gene comprising a high copy number, and a gene associated with cell apoptosis. Methods comprising DNA or RNA editing agents which elicit base editing are also disclosed.
A method of generating an RNA molecule having a silencing activity in a cell is provided, comprising: (a) identifying nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range, not including complete identity, with respect to nucleic acid sequences encoding RNA molecules engaged with RISC, (b) determining transcription of nucleic acid sequences encoding RNA molecules so as to select transcribable nucleic acid sequences encoding RNA molecules; (c) determining processability into small RNAs of transcripts of transcribable nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range so as to select transcribable nucleic acid sequences encoding aberrantly processed RNA molecules exhibiting predetermined sequence homology range; (d) modifying a nucleic acid sequence of aberrantly processed, transcribable nucleic acid sequences so as to impart processability into small RNAs that are engaged with RISC and are complementary to a first target RNA or to a target RNA of interest.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, wherein the gene encoding or processed into the non-coding RNA molecule is positioned in a coding gene, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Target RNA of interest include, for example, a transcript of a gene selected from the group consisting of a housekeeping gene, a dominant gene, a gene comprising a high copy number, and a gene associated with cell apoptosis. Methods comprising DNA or RNA editing agents which elicit base editing are also disclosed.
A method of producing a long dsRNA molecule in a plant cell that is capable of silencing a pest gene is provided, the method comprising: (a) selecting m a genome of a plant a nucleic acid sequence encoding a silencing molecule having a plant gene as a target, the silencing molecule capable of recruiting RNA-dependent RNA Polymerase (RdRp); and (b) modifying a nucleic acid sequence of the plant gene so as to impart a silencing specificity towards the pest gene, such that a transcript of the plant gene comprising the silencing specificity forms base complementation with said silencing molecule capable of recruiting said RdRp to produce the long dsRNA molecule capable of silencing the pest gene, thereby producing the long dsRNA molecule m the plant cell that is capable of silencing the pest gene.
A method of generating an RNA molecule having a silencing activity in a cell is provided, comprising: (a) identifying nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range, not including complete identity, with respect to nucleic acid sequences encoding RNA molecules engaged with RISC, (b) determining transcription of nucleic acid sequences encoding RNA molecules so as to select transcribable nucleic acid sequences encoding RNA molecules; (c) determining processability into small RNAs of transcripts of transcribable nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range so as to select transcribable nucleic acid sequences encoding aberrantly processed RNA molecules exhibiting predetermined sequence homology range; (d) modifying a nucleic acid sequence of aberrantly processed, transcribable nucleic acid sequences so as to impart processability into small RNAs that are engaged with RISC and are complementary to a first target RNA or to a target RNA of interest.
A method of producing a long dsRNA molecule in a plant cell that is capable of silencing a pest gene is provided, the method comprising: (a) selecting m a genome of a plant a nucleic acid sequence encoding a silencing molecule having a plant gene as a target, the silencing molecule capable of recruiting RNA-dependent RNA Polymerase (RdRp); and (b) modifying a nucleic acid sequence of the plant gene so as to impart a silencing specificity towards the pest gene, such that a transcript of the plant gene comprising the silencing specificity forms base complementation with said silencing molecule capable of recruiting said RdRp to produce the long dsRNA molecule capable of silencing the pest gene, thereby producing the long dsRNA molecule m the plant cell that is capable of silencing the pest gene.
A method of generating an RNA molecule having a silencing activity in a cell is provided, comprising: (a) identifying nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range, not including complete identity, with respect to nucleic acid sequences encoding RNA molecules engaged with RISC, (b) determining transcription of nucleic acid sequences encoding RNA molecules so as to select transcribable nucleic acid sequences encoding RNA molecules; (c) determining processability into small RNAs of transcripts of transcribable nucleic acid sequences encoding RNA molecules exhibiting predetermined sequence homology range so as to select transcribable nucleic acid sequences encoding aberrantly processed RNA molecules exhibiting predetermined sequence homology range; (d) modifying a nucleic acid sequence of aberrantly processed, transcribable nucleic acid sequences so as to impart processability into small RNAs that are engaged with RISC and are complementary to a first target RNA or to a target RNA of interest.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, with the proviso that said eukaryotic cell is not a plant cell, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule to a target RNA in a eukaryotic cell is also disclosed. Methods of disease prevention and treatment, methods of inducing cell apoptosis and methods of generating a eukaryotic non-human organism are also disclosed.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding at least one component of a caffeine biosynthesis pathway is disclosed. Methods of producing a coffee plant or part thereof, methods of producing coffee beans with reduced caffeine content, and methods of producing coffee with reduced caffeine content are also disclosed.
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding at least one component of a caffeine biosynthesis pathway is disclosed. Methods of producing a coffee plant or part thereof, methods of producing coffee beans with reduced caffeine content, and methods of producing coffee with reduced caffeine content are also disclosed.
42 - Scientific, technological and industrial services, research and design
Goods & Services
Genetic engineering services relating to plants; plant
engineering services; agricultural research; genetic
engineering services; scientific research in the field of
genetics and genetic engineering; testing, inspection and
research services in the fields of agriculture and breeding;
laboratory research in the field of gene expression; genetic
testing for scientific research purposes; technological
consultancy and research; information technology
engineering; product development consultancy in the fields
of molecular biology and molecular engineering.
Fruit and vegetable seeds for planting; agricultural seeds, seed potatoes; crop seeds; horticultural seeds, namely, seed germ and seeds for horticultural purposes, herb seeds for planting, grass seed, seeds for flowers, seeds for sowing; plant seeds; unprocessed coffee seeds, unprocessed cereal seeds, unprocessed edible seeds; living plants, natural plants and flowers, live annual flower plants; fresh fruits and vegetables
42 - Scientific, technological and industrial services, research and design
Goods & Services
Genetic engineering services relating to plants; plant engineering services; agricultural research; genetic engineering services; scientific research in the field of genetics and genetic engineering; testing, inspection and research services in the fields of agriculture and breeding; laboratory research in the field of gene expression; genetic testing for scientific research purposes; technological consultancy and research; information technology engineering; product development consultancy in the fields of molecular biology and molecular engineering
34.
MODIFYING THE SPECIFICITY OF NON-CODING RNA MOLECULES FOR SILENCING GENE EXPRESSION IN EUKARYOTIC CELLS
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, with the proviso that said eukaryotic cell is not a plant cell, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule to a target RNA in a eukaryotic cell is also disclosed. Methods of disease prevention and treatment, methods of inducing cell apoptosis and methods of generating a eukaryotic non-human organism are also disclosed.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, with the proviso that said eukaryotic cell is not a plant cell, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of said non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule to a target RNA in a eukaryotic cell is also disclosed. Methods of disease prevention and treatment, methods of inducing cell apoptosis and methods of generating a eukaryotic non-human organism are also disclosed.
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase. Also provided is a method of increasing extractability of solids from coffee beans. In addition there is provided a method of producing soluble coffee.
A coffee plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding alpha-D-galactosidase. Also provided is a method of increasing extractability of solids from coffee beans. In addition there is provided a method of producing soluble coffee.
A banana plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding a component in an ethylene biosynthesis pathway of the banana is provided. Also provides is a method of increasing shelf-life of banana.
A banana plant comprising a genome comprising a loss of function mutation in a nucleic acid sequence encoding a component in an ethylene biosynthesis pathway of the banana is provided. Also provides is a method of increasing shelf-life of banana.
Nucleic acid constructs for use in a method of selecting cells comprising a genome editing event, the method comprising (a) transforming cells of a plant of interest with the nucleic acid construct; (b) selecting transformed cells exhibiting fluorescence emitted by the fluorescent reporter using flow cytometry or imaging; and (c) culturing the transformed cells comprising the genome editing event by the DNA editing agent for a time sufficient to lose expression of the DNA editing agent so as to obtain cells which comprise a genome editing event generated by the DNA editing agent but lack DNA encoding the DNA editing agent.
