Abstract

A device incubates multiple patient samples using multiple incubation vessels, including a first unit having respective receptacles for respective incubation vessels, and a second unit having respective receptacles for respective incubation vessels. Both of the respective units are each movably mounted in their respective position along a common linear guide axis, and further include respective sensors for providing respective sensor signals, each of which indicates one of the respective positions, furthermore respective magnetic drive units for a respective change of a respective position, and furthermore a control unit designed to control the respective magnetic drive units on the basis of the respective sensor signals to cause the respective changes of the respective positions.

IPC Classes  ?

• G01N 35/10 - Devices for transferring samples to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

A method includes detecting in a sample a mammalian antibody binding specifically to ZSCAN5. A mammalian antibody binds specifically to ZSCAN5, and a carrier with a solid phase with at least one immobilized polypeptide includes at least one epitope of ZSCAN5 or a variant thereof. The mammalian antibody is used for diagnosing a neurological autoimmune disease or a cancer.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Goods & Services

Chemical, biochemical and biological agents and preparations used in industry, science and research; chemical, biochemical and biological agents and preparations used in industry, science and research, in particular proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations, carriers or carrier materials, in particular glass carriers, biochips, microbeads, nanobeads, microspheres, paper rings, chemical test papers, test strips, blots, in particular line blots or dot blots, microtiter plates or membranes, coated with proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations; diagnostic preparations, other than for medical or veterinary purposes; diagnostic preparations for scientific purposes, in particular proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations on a carrier or carrier material, in particular a biochip, microbead, nanobead, microsphere, paper ring, chemical test paper, test strip, blot, in particular line blot or dot blot, microtiter plate or membrane; reagents for synthesis, detecting, labelling, sequencing or fragment analysis of proteins, polypeptides, peptides or nucleic acids; polymeric membrane materials for liquid separation or separation of biochemical agents. Pharmaceuticals; medical preparations and veterinary preparations and medical agents and veterinary agents, in particular diagnostic preparations and diagnostic agents for medical or veterinary purposes; diagnostic preparations and diagnostic agents for medical or veterinary purposes for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, endocrine diseases, food intolerance or cancer; diagnostic preparations for use in laboratories; diagnostic preparations for medical or veterinary purposes, in particular proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations on a carrier or carrier material, in particular a biochip, microbead, nanobead, microsphere, paper ring, chemical test paper, test strip, blot, in particular line blot or dot blot, microtiter plate or membrane; test kits for medical or veterinary diagnostic purposes; test kits, in particular immunoserological, histological, histochemical, immunobiological or biochemical test kits, for medical or veterinary diagnostic purposes, comprising reagents that are matched to one another for conducting diagnostic tests; test kits for medical or veterinary diagnostic purposes, comprising proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations on a carrier or carrier material, in particular a biochip, microbead, nanobead, microsphere, paper ring, chemical test paper, test strip, blot, in particular line blot or dot blot, microtiter plate or membrane; reagents for medical or veterinary diagnostic purposes; reagents for medical or veterinary diagnostic purposes, in particular for the detection of allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, endocrine diseases, food intolerance or cancer; reagents for medical or veterinary diagnostic purposes, in particular proteins, polypeptides, peptides, lipids, carbohydrates, nucleic acids, antigens, allergens, cells, bacteria, viruses, lysates of microorganisms or pathogens, or tissue preparations on a carrier or carrier material, in particular a biochip, microbead, nanobead, microsphere, paper ring, chemical test paper, test strip, blot, in particular line blot or dot blot, microtiter plate or membrane. Scientific instruments for diagnosis, in particular for preparing, processing and evaluating diagnostic samples; Scientific instruments for diagnosis, in particular for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, food intolerance and cancer; Diagnostic apparatus not for medical purposes, in particular for preparing, processing and evaluating diagnostic samples; Diagnostic apparatus not for medical purposes, in particular for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, food intolerance and cancer; Diagnostic apparatus not for medical purposes, in particular chemiluminescence diagnostic apparatus, immune fluorescence diagnostic apparatus, line blot diagnostic apparatus, immune blot diagnostic apparatus, enzyme-linked immunosorbent diagnostic apparatus, molecular genetic diagnostic apparatus; Diagnostic apparatus for research laboratory use, in particular chemiluminescence diagnostic apparatus, immune fluorescence diagnostic apparatus, line blot diagnostic apparatus, immune blot diagnostic apparatus, enzyme-linked immunosorbent diagnostic apparatus, molecular genetic diagnostic apparatus; Testing apparatus for diagnostic purposes other than medical, in particular chemiluminescence testing apparatus, immune fluorescence testing apparatus, line blot testing apparatus, immune blot testing apparatus, enzyme-linked immunosorbent testing apparatus, molecular genetic testing apparatus; Measuring apparatus not for medical purposes, in particular chemiluminescence measuring apparatus, immune fluorescence measuring apparatus, line blot measuring apparatus, immune blot measuring apparatus, enzyme-linked immunosorbent measuring apparatus, molecular genetic measuring apparatus; Laboratory instruments, in particular chemiluminescence laboratory apparatus, immune fluorescence laboratory apparatus, line blot laboratory apparatus, immune blot laboratory apparatus, enzyme-linked immunosorbent laboratory apparatus, molecular genetic laboratory apparatus; Microscopes not for medical purposes, in particular immune fluorescence microscopes; Downloadable software for scientific, research, laboratory or diagnostic use; downloadable cloud-based software for scientific, research, laboratory or diagnostic use; downloadable software for managing, processing and analysing diagnostic and patient data; Software for scientific, research, laboratory or diagnostic use; Cloud-based software for scientific, research, laboratory or diagnostic use; Software for managing, processing and analysing diagnostic and patient data; Microscopes for medical use, in particular immune fluorescence microscopes. Scientific instruments for medical use, in particular for preparing, processing and evaluating diagnostic samples; Scientific instruments for medical use, in particular for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, food intolerance and cancer; Diagnostic apparatus for medical use, in particular for preparing, processing and evaluating diagnostic samples; Diagnostic apparatus for medical use, in particular for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases, metabolic diseases, food intolerance and cancer; Diagnostic apparatus for medical use, in particular chemiluminescence diagnostic apparatus, immune fluorescence diagnostic apparatus, line blot diagnostic apparatus, immune blot diagnostic apparatus, enzyme-linked immunosorbent diagnostic apparatus, molecular genetic diagnostic apparatus; Testing apparatus for medical use, in particular chemiluminescence testing apparatus, immune fluorescence testing apparatus, line blot testing apparatus, immune blot testing apparatus, enzyme-linked immunosorbent testing apparatus, molecular genetic testing apparatus; Measuring apparatus for medical use, in particular chemiluminescence measuring apparatus, immune fluorescence measuring apparatus, line blot measuring apparatus, immune blot measuring apparatus, enzyme-linked immunosorbent measuring apparatus, molecular genetic measuring apparatus; Laboratory instruments for medical use, in particular chemiluminescence laboratory apparatus, immune fluorescence laboratory apparatus, line blot laboratory apparatus, immune blot laboratory apparatus, enzyme-linked immunosorbent laboratory apparatus, molecular genetic laboratory apparatus.

Abstract

A method for enhancing the chemiluminescent signal of a chemiluminogenic compound in a chemiluminescent reaction mixture in a chemiluminescent reaction which has a step of oxidizing the chemiluminogenic compound in the presence of an effective amount of an enhancer can be performed. To obtain an enhanced chemiluminescent signal, a compound selected from the group of quaternary amine cationic substances of formula (I) for enhancing the chemiluminescent signal of an acridinium compound is used, preferably an acridinium ester or an acridinium sulfonamide. The signal is obtained in a chemiluminescent reaction, preferably in a chemiluminescent immunoassay, a related chemiluminescent composition and a related kit.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• C09K 11/07 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials having chemically-interreactive components, e.g. reactive chemiluminescent compositions

Goods & Services

Software for use in laboratory automation; Mobile apps, for use in the following fields: Lab automation; Software for monitoring laboratory apparatus; Software for monitoring and controlling laboratory apparatus; Mobile apps for monitoring laboratory apparatus; Mobile apps for monitoring and controlling laboratory apparatus; Software for monitoring diagnostic laboratory apparatus for medical purposes; Software for monitoring and controlling diagnostic laboratory apparatus for medical purposes; Mobiles apps for monitoring diagnostic laboratory apparatus for medical purposes; Mobile apps for monitoring and controlling diagnostic laboratory apparatus for medical purposes.

Abstract

A method is proposed for detecting a presence of a fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially comprises primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining respective items of location information, which indicate respective locations of respective relevant subsections of the cell substrate in the fluorescence image, and determining respective first partial confidence measures of respective presences of the fluorescence pattern on the respective subsections by means of a first neural network on the basis of the overall fluorescence image, extracting respective image subsections, which correspond to the respective subsections of the cell substrate, from the fluorescence image on the basis of the items of location information, determining respective second partial confidence measures of respective presences of the fluorescence pattern on the respective subsections by means of a second neural network on the basis of the respective image subsections, determining a confidence measure of the presence of the fluorescence pattern in the fluorescence image on the basis of the first partial confidence measures and the second partial confidence measures.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G06T 7/73 - Determining position or orientation of objects or cameras using feature-based methods
• G06V 10/82 - Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts

Abstract

A method is proposed for detecting at least one fluorescence pattern on an immunofluorescence image of a biological cell substrate, comprising the following steps: incubating the cell substrate with a liquid patient sample, which potentially includes primary antibodies, and furthermore with secondary antibodies, which are marked using a fluorescence stain, irradiating the cell substrate using excitation radiation and capturing the immunofluorescence image, determining segmentation information comprising at least one first and one second segmentation area, wherein the segmentation areas each represent a respective cell substrate area, via segmentation of the immunofluorescence image using a first neural network, determining a boundary area, which represents a transition from the first cell substrate area towards the second cell substrate area in the fluorescence image, on the basis of the segmentation information, selecting multiple partial images from the immunofluorescence image along the boundary area, determining a confidence measure of a presence of the fluorescence pattern on the basis of the multiple partial images via a second neural network.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• G06T 7/00 - Image analysis
• G06T 7/136 - SegmentationEdge detection involving thresholding

Goods & Services

Diagnostic preparations for medical and veterinary purposes; diagnostic preparations for medical and veterinary purposes, in particular recombinantly or synthetically produced antigens, lysates of microorganisms, preparations for conducting serological tests, biochemical preparations for conducting confirmatory tests; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins on a carrier/carrier material, in particular a biochip, microbead, nanobead, microsphere, test strip, blot, microtiter plate or membrane; diagnostic preparations for medical and veterinary purposes, in particular immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a biochip, microbead, nanobead, microsphere, test strip, blot, microtiter plate or membrane; immunoserological test kits, comprising reagents that are matched to one another for conducting immunoserological tests; test kits for medical and veterinary diagnostic purposes; test kits for medical and veterinary diagnostic purposes, comprising reagents that are matched to one another for conducting diagnostic tests; test kits for medical and veterinary diagnostic purposes, comprising immobilised proteins on a carrier/carrier material, in particular a microbead or a nanobead; test kits for medical and veterinary diagnostic purposes, comprising immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a microbead or a nanobead; test kits for medical and veterinary diagnostic purposes, comprising surface modified or surface activated microbeads or nanobeads enabling the coupling of proteins, polypeptides and peptides; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins on a carrier/carrier material, in particular a biochip, microbead, nanobead, microsphere, test strip, blot, microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes, in particular immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a biochip, microbead, nanobead, microsphere, test strip, blot, microtiter plate or membrane; all aforementioned goods not for or in connection with ophthalmic and ophthalmological pharmaceutical products.

Abstract

Provide are a diagnostically useful carrier coated with a recombinant polypeptide comprising SEQ ID NO: 1, an isolated autoantibody binding specifically to a polypeptide having SEQ ID NO: 1, a kit comprising the carrier, a method for the diagnosis of a membranous nephropathy (MN) that includes detecting the presence or absence of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 in a liquid sample comprising antibodies from a subject, a use of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 or a polypeptide comprising SEQ ID NO: 1, and a aqueous solution comprising an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Abstract

A method according to the invention is proposed for filtering a sensor signal, said method comprising various steps. A time-discrete sensor signal is provided as an input signal, and an output signal is generated via a continuous filtering of the input signal using a time-discrete filter in the time domain, which performs the following filter steps for each value of the input signal: comparing a specified signal value of a specified time index of the input signal with two further signal values of the input signal, wherein a first signal value of the two further signal values is temporally prior to the specified signal value and wherein a second signal value of the further signal values is temporally after the specified signal value, and replacing the specified signal value in the output signal by a new value in dependence on a value interval spanned by the two further signal values.

IPC Classes  ?

• G05B 19/4155 - Numerical control [NC], i.e. automatically operating machines, in particular machine tools, e.g. in a manufacturing environment, so as to execute positioning, movement or co-ordinated operations by means of programme data in numerical form characterised by programme execution, i.e. part programme or machine function execution, e.g. selection of a programme
• B25J 13/08 - Controls for manipulators by means of sensing devices, e.g. viewing or touching devices

Abstract

A method according to the invention is proposed for filtering a sensor signal, said method comprising various steps. A time-discrete sensor signal is provided as an input signal, and an output signal is generated via a continuous filtering of the input signal using a time-discrete filter in the time domain, which performs the following filter steps for each value of the input signal: comparing a specified signal value of a specified time index of the input signal with two further signal values of the input signal, wherein a first signal value of the two further signal values is temporally prior to the specified signal value and wherein a second signal value of the further signal values is temporally after the specified signal value, and replacing the specified signal value in the output signal by a new value in dependence on a value interval spanned by the two further signal values.

IPC Classes  ?

• G01D 3/032 - Measuring arrangements with provision for the special purposes referred to in the subgroups of this group mitigating undesired influences, e.g. temperature, pressure affecting incoming signal, e.g. by averagingMeasuring arrangements with provision for the special purposes referred to in the subgroups of this group mitigating undesired influences, e.g. temperature, pressure gating undesired signals
• G01D 5/24 - Mechanical means for transferring the output of a sensing memberMeans for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for convertingTransducers not specially adapted for a specific variable using electric or magnetic means influencing the magnitude of a current or voltage by varying capacitance
• G05B 1/03 - Comparing elements, i.e. elements for effecting comparison directly or indirectly between a desired value and existing or anticipated values electric for comparing digital signals

Abstract

An apparatus is provided for holding down membrane elements in respective wells of a multiwell plate, which has, at its top side, respective openings of the respective wells. The apparatus has, at its upper region, a support structure having respective openings, and respective corresponding retention elements which extend downwards from the respective openings of the support structure, so that respective retention elements protrude into respective wells when the support structure has been placed onto the top side of the multiwell plate. A retention element has multiple webs which extend downwards from the support structure and the ends of which are connected to one another at a bottom side of the apparatus by means of a connection element. Furthermore, a retention element has multiple openings between the webs, which openings extend continuously from the support structure right up to the connection element and which openings furthermore extend continuously from the top side of the multiwell plate right up to the connection element when the support structure has been placed onto the top side of the multiwell plate.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

Netrin G1 is disclosed as a biomarker of membranous glomerulonephritis (MGN). Netrin G1 antigen-comprising polypeptides and Netrin G1 antibodies can be used in in vitro methods and kits for diagnosis, prognosis and monitoring of MGN of patients with circulating Netrin G1 autoantibodies.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Goods & Services

Apparatus for recording, transmission or reproduction of diagnostic data, sound or images, in particular cameras, microscopes, in particular fluorescence microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, photomultipliers, microplate readers, software, in particular laboratory software and immunofluorescence positioning software; Microscopes for medical examinations and medical diagnoses, in particular fluorescence microscopes. Medical apparatus and instruments for medical examinations and medical diagnosis; Measuring, detecting and monitoring instruments, measuring, detecting and monitoring equipment and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, infectious diseases, autoimmune diseases and cancer.

Abstract

There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type. What further takes place is determination, on the basis of the previously determined segmentation information, of at least one sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type, determination, on the basis of the previously determined at least one sub-area, of validity information indicating a degree of a validity of the measure of confidence, and output of the measure of confidence depending on the validity information.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G06T 7/11 - Region-based segmentation
• G06T 7/62 - Analysis of geometric attributes of area, perimeter, diameter or volume

Goods & Services

Diagnostic preparations for medical and veterinary purposes; Diagnostic preparations for medical and veterinary purposes, Especially Recombinantly or synthetically produced antigens, Lysates of microorganisms, Preparations for conducting serological tests, Biochemical preparations for conducting confirmatory tests; Diagnostic preparations for medical and veterinary purposes, Especially Immobilised proteins on a carrier/carrier material, in particular a biochip, microsphere, nanosphere, micro sphere, test strip, blot, microtiter plate or membrane; Diagnostic preparations for medical and veterinary purposes, Especially Immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a biochip, microsphere, nanosphere, micro sphere, test strip, blot, microtiter plate or membrane; Immunoserological test kits, comprising reagents that are matched to one another for conducting immunoserological tests; diagnostic test kits for veterinary and medical use; diagnostic test kits for veterinary and medical use, Whereby the aforesaid goods comprise reagents that are matched to one another for conducting diagnostic tests; diagnostic test kits for veterinary and medical use, Whereby the aforesaid goods comprise immobilised proteins on a carrier/carrier material, in particular a microsphere or a nanosphere; diagnostic test kits for veterinary and medical use, Whereby the aforesaid goods comprise immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a microsphere or a nanosphere; diagnostic test kits for veterinary and medical use, Whereby the aforesaid goods comprise surface modified or surface activated microspheres or nanospheres enabling the coupling of proteins, polypeptides and peptides; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnostic purposes, Especially Immobilised proteins on a carrier/carrier material, in particular a biochip, microsphere, nanosphere, micro sphere, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes, Especially Immobilised peptides or immobilised polypeptides on a carrier/carrier material, in particular a biochip, microsphere, nanosphere, micro sphere, test strip, blot, microtiter plate or membrane; None of the aforesaid goods being intended for or in connection with ophthalmic and ophthalmological pharmaceutical goods.

Abstract

The present invention relates to a method for enhancing the chemiluminescent signal of a chemiluminogenic compound in a chemiluminescent reaction mixture in a chemiluminescent reaction which comprises oxidizing the chemiluminogenic compound in the presence of an effective amount of an enhancer to obtain an enhanced chemiluminescent signal, use of a compound selected from the group consisting of quarternary amine cationic substances of formula (I) for enhancing the chemiluminescent signal of an acridinium compound, preferably an acridinium ester or an acridinium sulfonamide, in a chemiluminescent reaction, preferably in a chemiluminescent immunoassay, a related chemiluminescent composition and a related kit.

IPC Classes  ?

• C09B 15/00 - Acridine dyes
• C09B 67/22 - Mixtures of different pigments or dyes or solid solutions of pigments or dyes
• C09K 11/07 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials having chemically-interreactive components, e.g. reactive chemiluminescent compositions
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Abstract

The present invention relates to a method for enhancing the chemiluminescent signal of a chemiluminogenic compound in a chemiluminescent reaction mixture in a chemiluminescent reaction which comprises oxidizing the chemiluminogenic compound in the presence of an effective amount of an enhancer to obtain an enhanced chemiluminescent signal, use of a compound selected from the group consisting of quarternary amine cationic substances of formula (I) for enhancing the chemiluminescent signal of an acridinium compound, preferably an acridinium ester or an acridinium sulfonamide, in a chemiluminescent reaction, preferably in a chemiluminescent immunoassay, a related chemiluminescent composition and a related kit.

IPC Classes  ?

• C09K 11/07 - Luminescent, e.g. electroluminescent, chemiluminescent, materials containing organic luminescent materials having chemically-interreactive components, e.g. reactive chemiluminescent compositions
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• C09B 67/22 - Mixtures of different pigments or dyes or solid solutions of pigments or dyes
• C09B 15/00 - Acridine dyes

Abstract

A method is used for the qualitative evaluation of real-time PCR data, where a time/PCR amplification plot of an associated sample is classified as a negative plot or as a positive plot. The method involves providing a real-time PCR amplification plot to be classified, plotting at least 20 successive amplitude values of corresponding successive PCR cycle indices of the sample. Next, a quality metric is determined, on the basis of the at least one amplitude value. A first criterion is determined by a comparison of the quality metric with a first standard value. A sequence of values is then determined, which indicates a gradient of the PCR amplification plot to be classified, and a second criterion is determined as to whether the sequence of values exceeds a second standard value. Finally, the real-time PCR amplification plot is classified as a positive plot if all the criteria given above are satisfied.

IPC Classes  ?

• G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
• G16B 25/20 - Polymerase chain reaction [PCR]Primer or probe designProbe optimisation
• C12Q 1/6851 - Quantitative amplification

Abstract

A method for detecting an error state when aspirating a liquid to identify whether a pre-determined volume of liquid was aspirated. The method including: immersing a tip of an aspiration needle in the liquid, aspirating a predetermined partial volume of the liquid in the aspiration needle, continuously acquiring a sensor signal curve, which indicates a pressure in the aspiration needle, during an overall time period, detecting the error state in the case that the sensor signal falls below a first threshold value during the predetermined time period, characterized by providing a reference signal curve, determining a deviation measure, which indicates a deviation of the sensor signal curve from the reference signal curve during the further time period, providing a predetermined second threshold value, detecting the error state as a function of the deviation measure and the second threshold value.

IPC Classes  ?

• G01L 19/00 - Details of, or accessories for, apparatus for measuring steady or quasi-steady pressure of a fluent medium insofar as such details or accessories are not special to particular types of pressure gauges
• G01L 27/00 - Testing or calibrating of apparatus for measuring fluid pressure

Abstract

A method for detecting at least one potential presence of at least one fluorescence pattern type on an organ section by means of immunofluorescence microscopy and by means of digital image processing is disclosed. An organ section is provided on a slide and the section is incubated with a liquid patient sample which potentially includes primary antibodies and with secondary antibodies which have been labelled with a fluorescent dye. A fluorescence image of the organ section is acquired. A determination is made, by segmentation of the fluorescence image by means of a first neural network, of a sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type. A determination is made, on the basis of the fluorescence image by means of a second neural network, of the measure of confidence that indicates an actual presence of the fluorescence pattern type. A determination is made, on the basis of the previously determined sub-area, of validity information that indicates a degree of a validity of the measure of confidence. And the measure of confidence of the actual presence of the fluorescence pattern type and of the validity information is outputted.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G06N 3/04 - Architecture, e.g. interconnection topology
• G06T 7/00 - Image analysis
• G06T 7/11 - Region-based segmentation

Abstract

An apparatus is proposed for identifying respective cover slip regions of respective cover slips having respective tissue sections on a specimen slide, which has multiple optical identifiers. The apparatus includes a planar light source, an image acquisition unit, a holding unit for positioning the specimen slide between the planar light source and the image acquisition unit, a slit diaphragm, which has multiple opening slits, reversibly positionable between the planar light source and the specimen slide, and an illumination unit, which is designed to illuminate that surface of the specimen slide which faces toward the image acquisition unit. Furthermore, the apparatus includes a monitoring unit which is designed, on the basis of a completely illuminated transmitted light image, an incident light image, and a partially darkened transmitted light image, to assign respective tissue sections to respective optical identifiers.

IPC Classes  ?

• G02B 21/08 - Condensers
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G06T 7/13 - Edge detection
• G06V 10/28 - Quantising the image, e.g. histogram thresholding for discrimination between background and foreground patterns
• G06V 10/50 - Extraction of image or video features by performing operations within image blocksExtraction of image or video features by using histograms, e.g. histogram of oriented gradients [HoG]Extraction of image or video features by summing image-intensity valuesProjection analysis
• G06V 20/69 - Microscopic objects, e.g. biological cells or cellular parts

Abstract

A nucleic acid or peptide detection system that includes a carrier with at least two areas is provided. A method and kit that use and/or include the nucleic acid peptide detection system are also provided.

IPC Classes  ?

• C12Q 1/6832 - Enhancement of hybridisation reaction

Abstract

A method can be used for detecting, in a sample, an autoantibody binding to a polypeptide having the sequence SEQ ID NO: 1, SEQ ID NO: 11 or SEQ ID NO: 24. A carrier containing said polypeptide and an autoantibody binding to said polypeptide are useful. The autoantibody may be used for the diagnosis of a disease, and a method can be used for isolating an autoantibody binding to said polypeptide. The polypeptide and a kit containing said polypeptide are useful, and can be used for the manufacture of a kit or medical device. A method involves contacting a medical or diagnostic device containing said polypeptide with a buffered solution containing an antibody binding specifically to said polypeptide. A sample containing said autoantibody as a positive control and a diluted sample containing said autoantibody are useful.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A method involves detecting in a sample an autoantibody binding specifically to a mammalian VGLUT. An autoantibody binding specifically to a mammalian VGLUT and a diagnostically useful carrier with a solid phase with an immobilized polypeptide containing a mammalian VGLUT or a variant thereof, are useful. The autoantibody can be used for diagnosing a neurological autoimmune disease or a cancer.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/574 - ImmunoassayBiospecific binding assayMaterials therefor for cancer

Abstract

The present invention relates to a method for detecting in a sample an autoantibody binding to a polypeptide having the sequence SEQ ID N01, SEQ ID NO11 or SEQ ID N024, a carrier comprising said polypeptide, an autoantibody binding said polypeptide, a use of the autoantibody for the diagnosis of a disease, a method for isolating an autoantibody binding to said polypeptide, a kit comprising said polypeptide, a use of said polypeptide, said carrier or said autoantibody for the manufacture of a kit or medical device, a method comprising the step contacting a medical or diagnostic device comprising said polypeptide with a buffered solution comprising an antibody binding specifically to said polypeptide, a use of a sample comprising said autoantibody as a positive control and a diluted sample comprising said autoantibody.

IPC Classes  ?

• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
• C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof

Goods & Services

Measuring and detection devices for processing and evaluation of diagnostic samples, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Measuring and detection devices for processing and evaluation of diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Sensors and detectors, in particular fluorescence and chemical luminescence detectors, for processing diagnostic samples, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Sensors and detectors, in particular fluorescence and chemical luminescence detectors, for processing and evaluation of diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays. Medical apparatus and instruments for medical examination purposes and medical diagnoses for analysis of diagnostic samples, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Medical apparatus and instruments for medical examination purposes and medical diagnoses for analysis of diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Laboratory apparatus and automatic laboratory machines for protein, antigen and antibody analysis for medical examination purposes and medical diagnoses, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays; Laboratory apparatus and automatic laboratory machines for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer, in particular for protein, antigen and antibody analysis for medical examination purposes and medical diagnoses, in particular for protein, antigen and antibody analysis, in particular by means of immunofluorescence and enzyme-linked immunosorbent assays.

Abstract

A method and apparatus are provided for detecting respective potential presences of respective different cellular fluorescence pattern types on a biological cellular substrate including human epithelioma cells (HEp cells), wherein the fluorescence pattern types include different antinuclear antibody fluorescence pattern types. A method is also provided for detecting potential presences of different cellular fluorescence pattern types on a biological cellular substrate including human epithelioma cells by means of digital image processing, as well as a computing unit, a data network device, a computer program product and a data carrier signal therefor.

IPC Classes  ?

• G06T 7/11 - Region-based segmentation
• G01N 21/64 - FluorescencePhosphorescence
• G06N 3/04 - Architecture, e.g. interconnection topology
• G06T 7/00 - Image analysis

Goods & Services

Diagnostic preparations; Diagnostic agents and diagnostic substances for scientific purposes, especially Immobilized proteins, cells, allergens or viruses or components thereof, nucleic acids, biomarkers and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, a microtiter plate or a membrane; Reagents for the detection of proteins and protein compounds, in particular of antigens or antibodies, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or foods with animal, vegetable and/or non-animal components, in particular for scientific purposes; Reagents for detecting carbohydrates and carbohydrate compounds, in particular antigens, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or foods with animal, vegetable and/or non-animal components, in particular for scientific purposes; Reagents for the detection of lipids and lipid compounds, in particular antigens, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or foods with animal, vegetable and/or non-animal components, in particular for scientific purposes. Diagnostic agents, in particular diagnostic agents for medical and veterinary purposes; Diagnostic agents for medical purposes; Diagnostic agents for veterinary purposes; Diagnostic agents, in particular diagnostic agents for medical and veterinary purposes for the detection of allergies, neurological diseases, food intolerances, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic agents for medical and veterinary purposes, Immobilized proteins, cells or viruses or components thereof, nucleic acids, biomarkers and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, a microtiter plate or a membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnostic purposes, Immobilized proteins, cells or viruses or components thereof, nucleic acids, biomarkers, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, a microtiter plate or a membrane; Reagents for the detection of proteins and protein compounds, in particular of antigens or antibodies, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or foods with animal, vegetable and/or non-animal components; Reagents for detecting carbohydrates and carbohydrate compounds, in particular of antigens, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or of foods with animal, vegetable and/or non-animal components; Reagents for the detection of lipids and lipid compounds, in particular of antigens, in animal samples, in particular for veterinary medicine, or in food samples, in particular in food samples of animal foods, non-animal foods, vegetable foods or foods with animal, vegetable and/or non-animal components.

Abstract

A primer pair that includes a forward primer and a reverse primer is used to amplify a nucleic acid from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22. A nucleic acid capable of hybridizing specifically to a nucleic acid sequence from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22 is provided. A carrier that includes the nucleic acid is provided. A method can be used to detect in a sample a nucleic acid sequence including SEQ ID NO: 22 from a pathogen associated with a skin, hair, and nail infection. The primer pair, the nucleic acid, or the carrier may be useful for the diagnosis of a disease. A kit including the primer pair, the nucleic acid, and/or the carrier may be useful for the diagnosis of a disease.

IPC Classes  ?

• C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12Q 1/6851 - Quantitative amplification

Abstract

The present invention relates to a diagnostically useful carrier coated with a recombinant polypeptide comprising SEQ ID N01, an isolated autoantibody binding specifically to a polypeptide having SEQ ID N01, a kit comprising the carrier, a method for the diagnosis of a membranous nephropathy (MN) comprising the step detecting the presence or absence of an autoantibody binding specifically to a polypeptide having SEQ ID NO1 in a liquid sample comprising antibodies from a subject, a use of an autoantibody binding specifically to a polypeptide having SEQ ID NO1 or a polypeptide comprising SEQ ID N01, and a aqueous solution comprising an autoantibody binding specifically to a polypeptide having SEQ ID N01.

IPC Classes  ?

• C07K 11/00 - Depsipeptides having up to 20 amino acids in a fully defined sequenceDerivatives thereof
• C07K 16/40 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against enzymes
• C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof
• C12N 9/10 - Transferases (2.)
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• C12Q 1/48 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving transferase
• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/567 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent utilising isolate of tissue or organ as binding agent
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Goods & Services

Software, in particular laboratory software; Integrated software packages for use in the automation of laboratories; Software for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases or cancer; Laboratory software for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases or cancer. Scientific services and research, Especially Scientific services for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer; Medical laboratory services.

Abstract

There is proposed a method for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid using Crithidia luciliae cells by means of fluorescence microscopy, including the steps of: provision of a substrate which has multiple Crithidia luciliae cells, incubation of the substrate with the patient sample which potentially has the autoantibodies, incubation of the substrate with secondary antibodies which have each been labelled with a preferably green fluorescent dye, acquisition of a fluorescence image of the substrate, identification by means of a first pretrained convolutional neural network of respective sub-images in the one fluorescence image that each represent a Crithidia luciliae cell, furthermore respective processing of at least one subset of the respective sub-images by means of a second pretrained convolutional neural network for determining respective binding measures which indicate a respective extent of a binding of autoantibodies in a respective kinetoplast region of a respective Crithidia luciliae cell of a respective sub-image, and determination of an overall binding measure with regard to a binding of autoantibodies from the patient sample to double-stranded deoxyribonucleic acid on the basis of the respective binding measures.

IPC Classes  ?

• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/00 - Microscopes
• G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• G06T 7/00 - Image analysis
• G06N 3/04 - Architecture, e.g. interconnection topology
• G06T 7/90 - Determination of colour characteristics

Abstract

A kit or set including a planar sample carrier having an absorbent membrane layer suitable for absorbing liquid blood components is provided, and wherein at least a portion of the blood components is able to dry in the membrane layer, wherein the sample carrier is additionally provided on an outer side with an optically readable code, additionally including a closable cuboidal box having a bottom surface that has a rectangular base surface and that additionally has a first opening, additionally including a planar receiving element having a base surface that corresponds to the base surface of the bottom surface, wherein the receiving element has a second opening and a holder that together are designed such that, on mounting the sample carrier in the holder and subsequently inserting the receiving element into the box on the bottom surface of the box, the optical code is visible through the first opening in the bottom surface and also through the second opening in the receiving element.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 9/00 - Supporting devicesHolding devices

Abstract

The invention relates to a method for determining the immune status of a subject, more specifically whether the subject has been vaccinated with a composition comprising SEQ ID NO1 or a variant thereof which composition triggers the production of antibodies in the subject or had no previous exposure to SARS-CoV-2 or any component of it, comprising the step determining in a sample the presence or absence of an IgA class antibody to SEQ ID NO1, wherein the sample comprises a set of representative antibodies from the subject. The present invention also relates to method for determining whether a subject produces antibodies as a result of administration of a vaccine for distinguishing whether a subject produces antibodies as a result of administration of a vaccine or as a result of a previous corona virus infection, comprising the step detecting in a sample comprising antibodies from said subject the absence or presence of an antibody to SEQ ID NO1 and the absence or presence of an antibody to SEQ ID NO2.

IPC Classes  ?

• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Goods & Services

Diagnostic preparations, other than for medical or veterinary use; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a test tube, biochip, microsphere, paper ring, test strip, blot, (ELISA-) microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical and veterinary purposes; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a test tube, biochip, microsphere, test strip, blot, (ELISA-) microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a test tube, biochip, microsphere, paper ring, test strip, blot, (ELISA-) microtiter plate or membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Abstract

An image processing method is provided for displaying cells from a plurality of pathology images or overall images. A respective overall image represents a respective patient tissue sample or a respective patient cell sample. The method includes the steps of: providing the overall images, detecting individual cell images in the overall images by means of a computer-assisted algorithm, determining classification data by means of the computer-assisted algorithm, wherein the classification data indicate a respective unique mapping of a respective detected cell image to one of a plurality of cell classes, and wherein the classification data further have a respective measure of confidence in respect of the respective unique mapping, generating respective class images for the respective cell classes, wherein a class image of a cell class reproduces the cell images mapped to the cell class in a regular arrangement and with a predetermined order, and wherein further the order of the mapped cell images is chosen on the basis of the measures of confidence of the mapped cell images, and further, displaying a portion of at least one class image.

IPC Classes  ?

• G06T 7/00 - Image analysis
• G16H 30/40 - ICT specially adapted for the handling or processing of medical images for processing medical images, e.g. editing
• G06F 18/2431 - Multiple classes

Abstract

The present invention relates to a method for diagnosing a SARS-CoV-2 infection comprising the step of detecting the presence or absence of an antibody to SEQ ID NO: 1, preferably IgA class antibody, in a sample from a subject, a method for the differential diagnosis of a coronavirus infection, a use of an antibody to SEQ ID NO: 1, preferably IgA class antibody for diagnosing a SARS-CoV-2 infection or for the differential diagnosis of a coronavirus infection, preferably for distinguishing between a SARS- CoV-2, MERS and NL63, 229E, OC43 and HKU1 infection, and a kit comprising a polypeptide comprising SEQ ID NO: 1 or a variant thereof, preferably coated to a diagnostically useful carrier and one or more, preferably all reagents from the group comprising an antibody to SEQ ID NO: 1, a washing buffer, a means for detecting the presence of an antibody, preferably IgA class antibody, preferably a secondary antibody binding specifically to IgA class antibodies, preferably comprising a detectable label, and a dilution buffer.

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• C07K 14/165 - Coronaviridae, e.g. avian infectious bronchitis virus

Abstract

A method is useful for automated detection of antibodies in a liquid biological sample with an antigen chip having antigen spots which are applied thereon and which have an identical, common dye. The antigen spots form respective antigen spot sets which form corresponding respective, regular antigen spot patterns. Furthermore, reference spots comprising the identical dye are applied, and form a reference spot set which forms a regular reference pattern. The reference pattern differs with respect to its regularity from the antigen spot patterns. Through a first image information item which represents a color of the reference spots and of the antigen spots due to the identical, first dye and through a second image information item which represents a potential color of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by image processing.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Goods & Services

UV-fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Combination apparatus for washing samples and the detection thereof, namely for indirect immunofluorescence assays. Medical apparatus and instruments for medical diagnosis; Devices for protein, antigen and antibody analysis for medical diagnoses, in particular medical IFT fully automatic machines.

Abstract

An apparatus is proposed for detecting, on a specimen slide comprising a plurality of optical identifiers, coverslip regions of coverslips each carrying a histological section. The apparatus comprises a planar light source, an image detection unit, a holding unit for positioning the specimen slide between the planar light source and the image detection unit, a slit aperture that is reversibly positionable between the planar light source and the specimen slide and has a plurality of aperture slits, and an illumination unit designed to illuminate precisely that surface of the specimen slide facing the image capturing unit. Further, the apparatus comprises a control unit that is designed to assign optical identifiers to respective histological sections on the basis of a completely illuminated transmitted light image, a reflected light image, and a partially darkened transmitted light image.

IPC Classes  ?

• G02B 21/08 - Condensers
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• G06K 9/46 - Extraction of features or characteristics of the image
• G06K 9/38 - Quantising the analogue image signal
• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints

Abstract

According to the invention, a method is proposed for the qualitative evaluation of real-time PCR data, wherein a time/PCR amplification plot of an associated sample is classified as a negative plot or as a positive plot. A real-time PCR amplification plot to be classified plotting at least 20 successive amplitude values of corresponding successive PCR cycle indices of an associated sample is provided. Furthermore, a quality metric is determined, preferably an individual quality metric which indicates a similarity of the real-time PCR amplification plot to be classified to a straight line, on the basis of the at least one amplitude value. In a further step, a first criterion is determined by means of a comparison of the quality metric with a first standard value. In a further step, a sequence of values is determined which indicates a gradient of the PCR amplification plot to be classified, and a second criterion as to whether the sequence of values exceeds a second standard value is determined. Finally, the real-time PCR amplification plot is classified as a positive plot in the event that all the criteria given above are satisfied.

IPC Classes  ?

• G16B 25/20 - Polymerase chain reaction [PCR]Primer or probe designProbe optimisation
• G16B 40/10 - Signal processing, e.g. from mass spectrometry [MS] or from PCR
• G16H 50/00 - ICT specially adapted for medical diagnosis, medical simulation or medical data miningICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
• C12Q 1/6851 - Quantitative amplification
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

A method for diagnosing a disease can include detecting, in a sample from a patient, an autoantibody binding to Septin-7. A polypeptide comprising Septin-7 or a variant thereof can be used for the diagnosis of a disease. Preferably, the polypeptide is used to detect an autoantibody binding to Septin-7 in a sample. A kit is useful for the diagnosis of a disease. The kit may include a polypeptide that includes Septin-7 or a variant thereof or a medical device that includes a polypeptide that includes Septin-7 or a variant thereof and an autoantibody to Septin-7.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans

Goods & Services

Diagnostic preparations, other than for medical or veterinary use; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-) microtiter plate or a membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical and veterinary purposes; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a test strip, a blot, an (ELISA-) microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-)microtiter plate or a membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Goods & Services

(1) Diagnostic preparations for clinical laboratory use; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-) microtiter plate or a membrane; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-) microtiter plate or a membrane. (2) Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical laboratory use; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a test strip, a blot, an (ELISA-) microtiter plate or membrane; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a test strip, a blot, an (ELISA-) microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-)microtiter plate or a membrane; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-)microtiter plate or a membrane. (3) Instruments, devices, and regulators, namely, laboratory incubators, spectrum analyzers, immunoassay analyzer, for measuring, detecting and monitoring for medical and clinical laboratory use for preparing, processing and evaluating diagnostic samples, for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular biochip sensors and UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software for preparing, processing and evaluating diagnostic samples for detecting neurological diseases, infectious diseases, autoimmune diseases, allergies, metabolic diseases, food intolerances and cancer.

Goods & Services

Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical and veterinary purposes; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, test strip, blot, microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue, sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring, devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Abstract

Summary The present invention relates to a method for diagnosing a SARS-CoV-2 infection comprising the step of detecting the presence or absence of an antibody to SEQ ID NO: 1, preferably IgA class antibody, in a sample from a subject, a method for the differential diagnosis of a coronavirus infection, a use of an antibody to SEQ ID NO: 1, preferably IgA class antibody for diagnosing a SARS-CoV-2 infection or for the differential diagnosis of a coronavirus infection, preferably for distinguishing between a SARS- CoV-2, MERS and NL63, 229E, 0C43 and HKU1 infection, and a kit comprising a polypeptide comprising SEQ ID NO: 1 or a variant thereof, preferably coated to a diagnostically useful carrier and one or more, preferably all reagents from the group comprising an antibody to SEQ ID NO: 1, a washing buffer, a means for detecting the presence of an antibody, preferably IgA class antibody, preferably a secondary antibody binding specifically to IgA class antibodies, preferably comprising a detectable label, and a dilution buffer. Date Recue/Date Received 2021-02-19

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Abstract

The present invention relates to a carrier for microscopic immunofluorescence analysis coated with a eukaryotic cell overexpressing a polypeptide comprising human NMDA receptor or a variant thereof, a carrier comprising the cell and a mock-transfected cell, wherein the cell and the mock-transfected cell are spatially separated, a kit comprising the cell or the carrier and a use of the cell or the carrier or a polypeptide comprising human NMDA receptor or a variant thereof for producing an artefact-free signal for the detection of an autoantibody to human NMDA receptor.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/94 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving narcotics

Abstract

The present invention relates to a carrier for microscopic immunofluorescence analysis coated with a eukaryotic cell overexpressing a polypeptide comprising human NMDA receptor or a variant thereof, a carrier comprising the cell and a mock-transfected cell, wherein the cell and the mock-transfected cell are spatially separated, a kit comprising the cell or the carrier and a use of the cell or the carrier or a polypeptide comprising human NMDA receptor or a variant thereof for producing an artefact-free signal for the detection of an autoantibody to human NMDA receptor.

IPC Classes  ?

• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• G01N 33/567 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent utilising isolate of tissue or organ as binding agent

Goods & Services

(1) Diagnostic preparations for clinical laboratory use; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-) microtiter plate or a membrane; diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-) microtiter plate or a membrane. (2) Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical laboratory use; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a test strip, a blot, an (ELISA-) microtiter plate or membrane; diagnostic preparations for medical laboratory use; diagnostic preparations for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a test strip, a blot, an (ELISA-) microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-)microtiter plate or a membrane; reagents for medical and veterinary diagnostic purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, a microsphere, a paper ring, a test strip, a blot, an (ELISA-)microtiter plate or a membrane. (3) Instruments, devices, and regulators, namely, laboratory incubators, spectrum analyzers, immunoassay analyzer, for measuring, detecting and monitoring for medical and clinical laboratory use for preparing, processing and evaluating diagnostic samples, for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular biochip sensors and UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software for preparing, processing and evaluating diagnostic samples for detecting neurological diseases, infectious diseases, autoimmune diseases, allergies, metabolic diseases, food intolerances and cancer.

Abstract

A diagnostically useful carrier includes a peptide including the amino acid sequence set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a variant thereof. A kit, a composition, a detection method, use for detecting a neurological disease, a human autoantibody specifically binding to Drebrin and a therapeutic compound or combination for use in the treatment of a neurological use are also useful.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals

Goods & Services

(1) Biological, biochemical, serological and immunological assays and kits for use in fields of biological, biochemical, serological, immunological and diagnostic testing for medical and veterinary use; Diagnostic agents and diagnostic substances, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. (2) Diagnostic agents, in particular diagnostic agents for medical and veterinary purposes for detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; Diagnostic agents for medical and veterinary diagnostic use; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/carrier material, in particular a biochip, microsphere, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. (3) Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmission or reproduction of diagnostic data, sound and images, in particular cameras, microscopes, photometers, microplate photometers, Enzyme Linked Immunosorbent Assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software for evaluation of results of biological, biochemical, serological, immunological and diagnostic tests, administration and electronic archiving of images and data, production of work protocols, in particular for biological, biochemical, serological, immunological and diagnostic tests, secure and convenient data communication and processing, management of test requests or data analysis.

Abstract

The present invention relates to a method comprising the step detecting an autoantibody to PLA2R in a sample comprising antibodies from a patient at a concentration range including 0.5 to 14 RU/ml, wherein the sample is contacted with a polypeptide comprising PLA2R or a variant thereof under conditions that allow for the formation of a complex comprising the autoantibody and PLA2r, wherein the complex is immobilized and detected; a diagnostically useful carrier comprising recombinant and/or purified human PLA2R or a variant thereof, wherein the carrier is capable of capturing an autoantibody to PLA2R for detection at a concentration below 14 RU/ml, and wherein the carrier selected from the group comprising a Western Blot membrane and a bead, preferably a magnetic bead; and a kit comprising the diagnostically useful carrier according to claim 13 and a means for detecting an autoantibody present in a sample at a concentration range below 14 RU/ml, which is a secondary antibody labeled with a detectable radioactive or chemiluminescent label.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Abstract

The present invention relates to the use of a secondary antibody comprising a chemiluminescent label for increasing the diagnostic reliability of an assay comprising the step detecting an autoantibody against phospholipase-A2-receptor and a A kit comprising a bead comprising a secondary antibody recognizing IgG class antibodies and/or phospholipase-A2-receptor or a variant thereof, wherein the antibody and/or the phospholipase-A2-receptor comprises a chemiluminescent label, and a calibrator defining a lower chemiluminescence detection limit of at least 4, preferably a lower limit of at least 3, more preferably a lower limit of at least 2 CU/ml, as well as one or more, preferably all from the group comprising one or more calibrator solutions, a sample dilution buffer, a washing solution, a positive control, a negative control and a chemiluminescence trigger solution.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Goods & Services

Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Sensors and detectors, in particular UV, fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmission or reproduction of diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers; Equipment for analyzing nucleic acids; Software, In particular laboratory software; Inserts for microplates. Medical apparatus and instruments for medical examinations and medical diagnosis; Inserts for medical apparatus and instruments for medical examination purposes.

Abstract

A recombinant polypeptide can be used in the diagnosis of the presence of a Zika virus in a patient. The recombinant polypeptide includes SEQ ID NO: 1 or a variant thereof. The recombinant peptide may be a monomer, a dimer, or a hexamer.

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• A61K 39/00 - Medicinal preparations containing antigens or antibodies

Goods & Services

(1) Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. (2) Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances, and cancer; diagnostic preparations for medical laboratory use; diagnostic preparations, for medical and veterinary purposes in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier, in particular a biochip, microsphere, test strip, blot, microtiter plate or membrane; diagnostic preparations, for medical and veterinary purposes in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier material, in particular a biochip, microsphere, test strip, blot, microtiter plate or membrane; reagents for medical and veterinary diagnostic purposes; reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue, sections on a carrier, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue, sections on a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. (3) Instruments, devices, and regulators, namely, laboratory incubators, spectrum analyzers, immunoassay analyzer, for measuring, detecting and monitoring for medical and clinical laboratory use for preparing, processing and evaluating diagnostic samples, for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases, food intolerances and cancer; sensors and detectors, in particular biochip sensors and UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software for preparing, processing and evaluating diagnostic samples for detecting neurological diseases, infectious diseases, autoimmune diseases, allergies, metabolic diseases, food intolerances and cancer.

Abstract

A method is for detecting respective potential presence of respective different antinuclear antibody fluorescence pattern types on a biological cell substrate including human epithelioma cells, including: acquiring a first image which represents staining of the cell substrate by a first fluorescent dye, and acquiring a second image which represents staining of the cell substrate by a second fluorescent dye, detecting, on the basis of the first image, respective image segments which in each case represent at least one mitotic cell, selecting, on the basis of the detected image segments, subimages of the first image and subimages corresponding thereto of the second image and detecting, on the basis of the selected subimages of the first image and of the selected subimages of the second image, respective actual presence of respective cellular fluorescence pattern types by means of a convolutional neural network.

IPC Classes  ?

• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G06T 7/00 - Image analysis
• G06T 7/11 - Region-based segmentation
• G06T 7/70 - Determining position or orientation of objects or cameras
• G01N 15/10 - Investigating individual particles
• G06N 3/04 - Architecture, e.g. interconnection topology

Abstract

Toxocara canis larvae. Further, a kit, use, methods, and compositions that include the diagnostically useful carrier are disclosed.

IPC Classes  ?

• C12N 11/00 - Carrier-bound or immobilised enzymesCarrier-bound or immobilised microbial cellsPreparation thereof
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/567 - ImmunoassayBiospecific binding assayMaterials therefor using specific carrier or receptor proteins as ligand binding reagent utilising isolate of tissue or organ as binding agent
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans

Abstract

The present invention relates to a diagnostically useful carrier comprising a means for specifically capturing an antibody to a polypeptide from the group comprising squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject, a method comprising the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2 and a use of the polypeptide or the carrier according to the present invention or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

IPC Classes  ?

• A61K 38/17 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
• C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof
• C07K 19/00 - Hybrid peptides
• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Abstract

The invention relates to a sample support. The sample support has an absorbent membrane layer which is based on a nonwoven polyolefin and which has at least one take- up region with dried blood constituents. It moreover has a film layer based on a plastic, where the film layer has received antistatic pretreatment. The membrane layer and the film layer have been connected to one another mechanically in one subregion of the sample support, but the layers have moreover not been connected to one another mechanically in the take-up region. It is preferable that, when the sample support is placed onto a uniformly level surface the membrane layer covers the film layer at least in the take-up region.

IPC Classes  ?

• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• G01N 1/28 - Preparing specimens for investigation

Abstract

A recombinant or chemically synthesized polypeptide includes SEQ ID NO: 1 or a variant thereof. A diagnostically useful carrier includes means for specifically capturing an antibody to SEQ ID NO: 1 in a sample from a subject. A kit includes the polypeptide or the diagnostically useful carrier. A method for diagnosing, prognosing, or monitoring the treatment of a virus, preferably Flavivirus, more preferably POWV infection includes detecting in the sample from a subject the presence or absence of an antibody to SEQ ID NO: 1 and/or an antibody to SEQ ID NO: 2. A polypeptide including SEQ ID NO: 1 and/or SEQ ID NO: 2, and/or a variant thereof is useful for the diagnosis. An IgM antibody to SEQ ID NO: 1 or means for specifically capturing an IgM class antibody to SEQ ID NO: 1 is useful for increasing the diagnostic reliability of an immunoassay for the diagnosis of a virus infection.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• A61K 39/12 - Viral antigens
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• A61K 39/295 - Polyvalent viral antigensMixtures of viral and bacterial antigens
• A61K 39/395 - AntibodiesImmunoglobulinsImmune serum, e.g. antilymphocytic serum
• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

A method is used for optical scanning of at least one object region placed on a transparent specimen holder. The method is as follows: for each sample lateral position of plural predefined sample lateral positions performing a focus determination by: performing laser reflection and using a first camera taking plural first images to determine a reference distance between the specimen holder and an objective lens; performing transmission flash illumination and using a second camera taking plural second images to define a focus distance taking into account the reference distance; after completing the focus determination, determining a focus distance topology across the object region based on the focus distances determined for ail sample lateral positions; and laterally moving the specimen holder and acquiring third images while focusing according to the focus distance topology.

IPC Classes  ?

• G02B 21/24 - Base structure
• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/00 - Microscopes
• G02B 21/26 - StagesAdjusting means therefor

Goods & Services

Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, food intolerances, autoimmune diseases and cancer; Sensors and detectors, in particular UV, fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmission and reproducing diagnostic data, sound and images, in particular cameras, Microscopes, Photometers, Microdisk photometers, Enzyme-linked immunosorbent assay readers, Photomultiplier tubes, Microdisk readers; Software, In particular laboratory software and Software for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, food intolerances, autoimmune diseases and cancer. Scientific services and research, In particular scientific services for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, food intolerances, autoimmune diseases and cancer; Industrial analysis and research services, In particular research services for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, Food intolerances, autoimmune disease and cancer; Design and development of computer hardware and software, In particular laboratory software and software for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, Food intolerances, autoimmune disease and cancer.

Abstract

The present invention relates to a method for diagnosing a disease comprising the step detecting in a sample comprising antibodies from a patient an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a polypeptide comprising a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, or a variant thereof, a use of said polypeptide for the diagnosis of a disease, an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a use of the autoantibody for the diagnosis of a disease, a method for isolating an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a pharmaceutical composition or medical device comprising said polypeptide according to the present invention, a kit for the diagnosis of a disease comprising said polypeptide or said medical device and a use of said polypeptide or autoantibody for the manufacture of a kit or medical device.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• C07K 1/14 - ExtractionSeparationPurification
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans

Goods & Services

Diagnostic preparations; Diagnostic agents and diagnostic substances for scientific purposes, In particular immobilised proteins, Nucleic acids, Allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, (ELISA) microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, food intolerances, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic preparations for medical and veterinary purposes, In particular immobilised proteins, Nucleic acids, Allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, test strip, blot, (ELISA) microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnostic purposes, In particular immobilised proteins, Nucleic acids, Allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, (ELISA) microtiter plate or membrane.

Goods & Services

Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, food intolerances, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples, for detecting allergies, Neurological disorders, metabolic disorders, infectious diseases, autoimmune diseases, food intolerances and cancer; Sensors and detectors, in particular UV, fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Abstract

Crithidia luciliae cells by fluorescence microscopy and by digital image processing.

IPC Classes  ?

• G06K 9/00 - Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G02B 21/00 - Microscopes
• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• G06T 7/00 - Image analysis

Goods & Services

Diagnostic preparations; Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, food intolerances, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane.

Abstract

Trichinella bound to a signal molecule, wherein the antigens of (b) (ii) are configured so as to dissolve binding of the antigens of (a) to the first antibody by competitive displacement.

IPC Classes  ?

• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C07K 16/18 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans
• C07K 14/435 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/487 - Physical analysis of biological material of liquid biological material
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 33/12 - MeatFish
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A method and microscopy system are useful for recording an image of a sample region. A laser beam is directed onto the sample region with interface(s). An objective lens facilitates images the laser beam on a focusing point which lies on the optical axis of the objective lens or an axis parallel thereto, and which lies in a focusing plane. The objective lens and the sample region are displaced with respect to one another in relative fashion along the optical axis of the objective lens to different relative displacement positions. Intensity values of the laser beam are captured for a respective relative displacement position. A respective highest intensity value for a respective displacement position, a curve of the highest intensity values, and a reference relative displacement position from at least one maximum of the curve, are determined. Image(s) of the sample region is/are captured at the reference relative displacement position.

IPC Classes  ?

• G02B 21/06 - Means for illuminating specimen
• G02B 21/00 - Microscopes
• G02B 21/02 - Objectives
• G02B 21/24 - Base structure
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes

Goods & Services

Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer, sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmission and reproducing diagnostic data, sound and images, in particular cameras, Microscopes, Photometers, Microdisk photometers, Enzyme-linked immunosorbent assay readers, Photomultiplier tubes, Microdisk readers; Software, In particular laboratory software, Software for creating, reading and allocating barcodes; All goods used for processing dried blood and not for creating genetic, epidemiological or neurological cards and not for creating medical images, cards or diagrams and including not being suitable for the aforesaid. Scientific services and research, Especially Scientific services for evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer; Industrial analysis and research services, in particular research for detecting allergies, neurological diseases, metabolic disorders, infectious diseases, autoimmune diseases and cancer; Design and development of computer hardware and software, Especially Laboratory software, software for creating, reading and allocating barcodes; All services being associated with goods used for processing dried blood and not for creating genetic, epidemiological or neurological cards and not for creating medical images, cards or diagrams and including not being suitable for the aforesaid.

Abstract

A method is useful for recording a microscopic fluorescence image of a sample region. An objective directs a laser beam on the sample region having boundary surface(s). A relative distance between the objective and the sample region is altered along an optical axis of the objective to effectuate respective, different relative distances. A respective set of pixel intensity values are effectuated on sensor pixels of an image sensor by the laser beam and transmitted back through the objective is captured for a respective relative distance. A respective focus metric is determined for a respective relative distance based on the respective set of pixel intensity values captured for the respective relative distance. A preferred relative distance is determined based on the determined focus metrics. The preferred relative distance is set, the sample region is illuminated with excitation radiation and the microscopic fluorescence image is captured via the image sensor.

IPC Classes  ?

• G01N 21/64 - FluorescencePhosphorescence
• G02B 21/16 - Microscopes adapted for ultraviolet illumination
• G01N 21/359 - Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light

Abstract

A pressure-tight storage vessel contains a liquid, has an elongated main body rotationally symmetrical with respect to an axis of symmetry, and forms, at least partially, a rotationally symmetrical hollow space in which the liquid is substantially received, wherein the main body is terminated at its bottom side by a base. Furthermore, at its top side, the storage vessel has an opening which is closed off in a pressure-tight manner by a closure, has a plurality of reinforcement elements, which bears against the main body at the outside, which extends parallel to the axis of symmetry of the main body, and arranged rotationally symmetrically about the axis of symmetry of the main body. In each case between adjacent reinforcement elements, respective externally exposed wall sections of the main body are formed, and the composition of the exposed wall sections permits a pressure-tight insertion by at least two hollow needles.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B65B 1/16 - Methods of, or means for, filling the material into the containers or receptacles by pneumatic means, e.g. by suction

Abstract

Disclosed herein is a pressure-tight storage vessel containing a liquid, having an elongate main body rotationally symmetrical with respect to an axis of symmetry and which forms a rotationally symmetrical hollow space in which the liquid is substantially received, wherein the main body is terminated at its bottom side by a base and has a top side opening closed off in a pressure-tight manner by a closure, having a plurality of reinforcement elements which bear against the main body at the outside and which extend parallel to the axis of symmetry and which are arranged rotationally symmetrically about the axis of symmetry such that, in each case between adjacent reinforcement elements, respective extemally exposed wall sections of the main body are formed, and wherein the composition of the exposed wall sections permits pressure-tight insertion by at least two hollow needles.

IPC Classes  ?

• B29C 45/00 - Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mouldApparatus therefor
• B29C 64/00 - Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
• B33Y 80/00 - Products made by additive manufacturing
• B65D 1/09 - Ampoules
• B65D 1/42 - Reinforcing or strengthening parts or members
• B65D 85/00 - Containers, packaging elements or packages, specially adapted for particular articles or materials

Abstract

A method is useful for diagnosing AIG, which includes the step of detecting whether autoantibodies against the beta-subunit of the gastric H+/K+-ATPase are present or not in a sample comprising antibodies. The method utilizes a diagnostically useful carrier that contains a solid phase on which an agent for the specific capture of the autoantibody is immobilized. Furthermore, the diagnostically useful carrier includes a solid phase on which an agent for the specific capture of an autoantibody against the beta-subunit of the gastric H+/K+-ATPase is immobilized.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease

Goods & Services

Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Sensors and detectors, in particular UV, fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmission or reproduction of diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers; Equipment for analyzing nucleic acids; Software, In particular laboratory software. Medical apparatus and instruments for medical examinations and medical diagnosis; Devices for protein, antigen and antibody analysis for medical examination purposes and medical diagnoses, in particular medical enzyme linked immunosorbent assay readers and chemiluminescent readers.

Abstract

A polypeptide contains laminin beta-4 and a carrier contains the polypeptide. An antibody, preferably autoantibody, is against laminin beta-4. Furthermore, use of the polypeptide, carrier or autoantibody for the diagnosis of a disease, and a method with the step of detecting an autoantibody against laminin beta-4 in a sample are described.

IPC Classes  ?

• C07K 14/78 - Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• C07K 16/28 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C07K 17/00 - Carrier-bound or immobilised peptidesPreparation thereof
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

A method is used for diagnosing a disease by detecting in a sample with antibodies from a patient an autoantibody binding to DAGLA.

IPC Classes  ?

• G01N 33/564 - ImmunoassayBiospecific binding assayMaterials therefor for pre-existing immune complex or autoimmune disease
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 17/02 - Peptides being immobilised on, or in, an organic carrier
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A method is used for acquiring and displaying an immunofluorescence image of a biological sample. During a first operating state, the sample is continuously illuminated using the excitation radiation. A relative position between the sample and an optical system, which guides the fluorescent radiation to an image sensor, is changed in dependence on a movement gesture of a user, and a corresponding digital image is displayed. Upon detection of a termination of the movement gesture, a change is made from the first operating state to a second operating state. In the second operating state, the sample is firstly illuminated using the excitation radiation to excite the fluorescent radiation of the fluorescent pigment. An acquisition of fluorescent radiation emitted by the sample then takes place and a corresponding, further digital image is generated. In the second operating state, the acquisition of the emitted fluorescent radiation and the illumination of the sample are terminated after lapse of the second acquisition duration.

IPC Classes  ?

• G01N 15/14 - Optical investigation techniques, e.g. flow cytometry
• G02B 21/36 - Microscopes arranged for photographic purposes or projection purposes
• H04N 5/232 - Devices for controlling television cameras, e.g. remote control
• G01N 21/64 - FluorescencePhosphorescence
• G01N 33/483 - Physical analysis of biological material
• G02B 21/16 - Microscopes adapted for ultraviolet illumination
• G02B 21/26 - StagesAdjusting means therefor
• H04N 5/225 - Television cameras

Abstract

What is proposed is a method for the automated detection of antibodies in a liquid biological sample by means of use of an antigen chip containing antigen spots applied thereto, which antigen spots comprise the same, common dye. The antigen spots form respective antigen spot quantities that form corresponding respective, regular antigen spot patterns. Furthermore, reference spots containing the same dye are applied, which reference spots form a reference spot quantity that forms a regular reference pattern. The reference pattern differs from the antigen spot patterns with respect to its regularity. On the basis of a first item of image information that represents a coloration of the reference spots and of the antigen spots due to the same, first dye and on the basis of a second item of image information that represents a potential coloration of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by means of image processing.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

What is proposed is a method for the automated detection of antibodies in a liquid biological sample by means of use of an antigen chip containing antigen spots applied thereto, which antigen spots comprise the same, common dye. The antigen spots form respective antigen spot quantities that form corresponding respective, regular antigen spot patterns. Furthermore, reference spots containing the same dye are applied, which reference spots form a reference spot quantity that forms a regular reference pattern. The reference pattern differs from the antigen spot patterns with respect to its regularity. On the basis of a first item of image information that represents a coloration of the reference spots and of the antigen spots due to the same, first dye and on the basis of a second item of image information that represents a potential coloration of the antigen spots due to a second dye after an incubation, binding of antibodies of the biological sample to respective antigen types is then determined by means of image processing.

IPC Classes  ?

• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids

Abstract

A recombinant polypeptide can be used in the diagnosis of the presence of a Zika virus in a patient. The recombinant polypeptide includes SEQ ID NO1 or a variant thereof, where the recombinant polypeptide is a monomer, a dimer, or a hexamer.

IPC Classes  ?

• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

Planar sample carriers having a liquid-adsorbing material layer in order to receive a liquid patient sample therein, such as a drop of blood, are known. Following application of the liquid patient sample to a segment of the sample carrier, the sample may then dry on this segment of the sample carrier, so that the sample carrier together with the sample can be transferred to a laboratory for biochemical analysis. At a later time, this segment then has to be removed or separated from the rest of the sample carrier in order to be able to feed the segment together with the patient sample contained therein to a biochemical analysis process, such as a PCR (polymerase chain reaction). In accordance with the invention it is proposed to separate the segment by cutting the segment from the rest of the sample carrier by means of a laser beam of a certain wavelength. This cutting process is preferably supported in that the sample carrier or the material layer has cut-outs, such that the segment to be separated is connected to the rest of the sample carrier only by one or more webs.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood

Goods & Services

Diagnostic preparations; Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane.

Abstract

An incubation device has multiple rocking platforms with each platform pivotally mounted about a respective axis. Each rocking platform has a respective receiving device for mechanically reversibly receiving a respective incubation channel. The device also has a common drive unit for common respective rocking movements of each platform from a zero position through with a specific angle range as a result of movement of the drive unit. Each rocking platform is coupled to the drive unit via a respective coupling mechanism, and each coupling mechanism elastically and resiliently couples the respective rocking platform to the drive unit, such that when the rocking platform is restrained, despite continuation of the drive movement, the rocking platform remains motionless in a fixed position. After release of the restraint on the rocking platform, the rocking platform resumes the rocking movement with the specific angle range.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• G01N 1/31 - Apparatus therefor
• B01L 9/00 - Supporting devicesHolding devices
• B01F 31/23 - Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 1/00 - EnclosuresChambers
• B01L 7/04 - Heat insulating devices, e.g. jackets for flasks
• B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Abstract

An elongated incubation trough has an indentation open toward a top end as well as a bottom. The indentation has a first receiving area to receive an elongated test strip as well as a second receiving area to receive an end section of a fluid line. The second receiving area is in fluidic communication with the first receiving area. A maximum width of the second receiving area at bottom height is greater than a maximum width of the first receiving area at bottom height.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• B01L 9/00 - Supporting devicesHolding devices
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 35/00 - Automatic analysis not limited to methods or materials provided for in any single one of groups Handling materials therefor

Abstract

The present invention relates to a recombinant or chemically synthesized polypeptide comprising SEQ ID NO1 or a variant thereof, a diagnostically useful carrier comprising in means for specifically capturing an antibody to SEQ ID NO1 in a sample from a subject, a kit comprising the polypeptide or a diagnostically useful carrier, and a method for diagnosing, prognosing or monitoring the treatment of a virus, preferably Flavivirus, more preferably POWV infection comprising the step detecting in the sample from a subject the presence or absence of an antibody to SEQ ID NO1 and/or an antibody to SEQ ID NO2, and a use of a polypeptide comprising SEQ ID NO1 and/or a SEQ ID NO2 or a variant thereof for the diagnosis and a use of an IgM antibody to SEQ ID NO1 or in means for specifically capturing an IgM class antibody to SEQ ID NO1 for increasing the diagnostic reliability of an immuno-assay for the diagnosis of a virus infection.

IPC Classes  ?

• C07K 14/005 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from viruses
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses

Goods & Services

Diagnostic preparations; Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Goods & Services

Diagnostic preparations; Diagnostic preparations and diagnostic substances for scientific purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Diagnostic preparations, in particular diagnostic preparations for medical and veterinary purposes for the detection of allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Diagnostic preparations for medical and veterinary purposes; Diagnostic agents for medical and veterinary purposes, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane; Reagents for medical and veterinary diagnostic purposes; Reagents for medical and veterinary diagnosis, in particular immobilised proteins, nucleic acids, allergens and tissue sections on a carrier/a carrier material, in particular a biochip, microsphere, paper ring, test strip, blot, microtiter plate or membrane. Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Sensors and detectors, in particular UV fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmitting or reproducing diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers, software, in particular laboratory software.

Goods & Services

Measuring, detecting and monitoring instruments, measuring, detecting and monitoring devices, and measuring, detecting and monitoring regulators for preparing, processing and evaluating diagnostic samples for detecting allergies, neurological diseases, metabolic diseases, infectious diseases, autoimmune diseases and cancer; Sensors and detectors, in particular UV, fluorescence and chemiluminescence detectors for preparing, processing and evaluating diagnostic samples; Apparatus for recording, transmission or reproduction of diagnostic data, sound or images, in particular cameras, microscopes, photometers, microplate photometers, enzyme-linked immunosorbent assay readers, chemiluminescence readers, photomultipliers, microplate readers; Equipment for analyzing nucleic acids; Software, In particular laboratory software. Medical apparatus and instruments for medical examinations and medical diagnosis; Nucleic acid analysis apparatus for medical examination purposes and medical diagnoses.