An object of the present invention is to provide a method for determining that a subject is at risk of suffering or is suffering from COVID-19. The present invention provides a method for determining whether a subject that is a mammal is at risk of suffering or suffering from COVID-19, the method including a step of detecting the amount of at least one modified nucleoside selected from the group consisting of 6-threonylcarbamoyl adenosine (t6A) and 2-thiomethyl,6-threonylcarbamoyl adenosine (ms2t6A) in a sample derived from the subject.
NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY (Japan)
AISTI SCIENCE CO., LTD. (Japan)
Inventor
Kunisawa, Akihiro
Hattori, Takanari
Watanabe, Jun
Tomizawa, Kazuhito
Nagayoshi, Yu
Sasano, Ryoichi
Asai, Tomoki
Abstract
A method for analyzing a modified nucleoside according to the present invention comprises: an elution step for introducing a sample containing a target component that is a modified nucleoside of which the hydrophobicity is increased by modification and a reference component that is a different component from the target component into a liquid chromatography column, and then separating the target component and the reference component from each other and eluting the target component and the reference component from the column by gradient elution such that the mixing ratio of a plurality of solvents constituting a mobile phase is changed over time; a step for detecting the target component and the reference component separately by mass spectrometry; and a step for calculating the ratio of a detected value for the target component to a detected value for the reference component. In the elution step, the mixing ratio of the solvents constituting the mobile phase to be introduced into the column is changed in such a manner that a third period in which the change ratio of the mixing ratio of the solvents constituting the mobile phase as measured at an outlet port of the column is larger than that in a first period in which the target component is eluted from the column and that in a second period in which the reference component is eluted from the column is provided between the first period and the second period.
C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/58 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving urea or urease
C12Q 1/62 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving uric acid
C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6872 - Methods for sequencing involving mass spectrometry
G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosolsInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
G01N 30/26 - Conditioning of the fluid carrierFlow patterns
G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
G01N 30/50 - Conditioning of the sorbent material or stationary liquid
NATIONAL UNIVERSITY CORPORATION KUMAMOTO UNIVERSITY (Japan)
SHIMADZU CORPORATION (Japan)
AISTI SCIENCE CO., LTD. (Japan)
Inventor
Tomizawa, Kazuhito
Nagayoshi, Yu
Abstract
The purpose of the present invention is to provide a method for identifying a subject who is or may be suffering from COVID-19. The present invention provides a method for identifying whether or not a mammal subject is or may be suffering from COVID-19, the method comprising detecting the level of at least one modified nucleoside selected from the group consisting of 6-threonylcarbamoyladenosine (t6A) and 2-thiomethyl,6-threonylcarbamoyladenosine (ms2t6A) in a sample derived from the subject.
C12Q 1/34 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving hydrolase
G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosolsInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
4.
GASEOUS ODOR COMPONENT COLLECTION NEEDLE, AND GASEOUS ODOR COMPONENT MEASURING GAS CHROMATOGRAPH USING SAME
This gaseous odor component measuring gas chromatograph comprises: a gaseous odor component collection needle including a hollow thin tube in which an adsorption layer capable of adsorbing the gaseous odor component is formed on an inner wall surface; a nozzle including a connection part capable of connecting to the needle in an airtight state; a pump capable of suctioning, through the nozzle and the needle connected to the nozzle, gas from a sealed container in which the gas containing the gaseous odor component is sealed to cause the gaseous odor component in the gas to be adsorbed by the adsorption layer, and also of sending gas for discharging the adsorbed gaseous odor component from a tip of the needle; a drive mechanism that drives the nozzle; and a predetermined control unit. Due to the foregoing, the invention enables provision of technology with which the suction resistance is reduced when suctioning a sample gas containing an odor component from a sealed container to collect the gaseous odor component in the sample gas into the needle, and the collected gaseous odor component can be easily analyzed by the gas chromatograph.
G01N 1/00 - SamplingPreparing specimens for investigation
G01N 1/22 - Devices for withdrawing samples in the gaseous state
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
A liquid chromatograph includes: an analysis column; a sample loop that temporarily contains a mixed liquid of a sample and a mixture solvent; and a passage switch valve capable of switching a passage between a load position where the mixed liquid is temporarily held in the sample loop and an injection position where the mixed liquid held in the sample loop is sent to the analysis column. In the liquid chromatograph, a specific passage is formed in each of the load position and the injection position.
A pre-analysis treatment method includes: a first retaining step of supplying, to a solid phase, each predetermined amount of the sample and a first solvent, and allowing a component at low concentration to be retained in the solid phase; a first washing step of supplying the first solvent to the solid phase and removing a component at high concentration from the solid phase; a second retaining step of supplying the sample in a smaller amount than the predetermined amount supplied in the first retaining step, and a second solvent to the solid phase, and allowing the component at high concentration to be retained in the solid phase; and an elution step of simultaneously extruding the components retained in the solid phase with an extrusion solvent.
B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
G01N 30/96 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography using ion-exchange
A pre-analysis treatment device usable for an amino acid, organic acid, and glucide includes an ion-exchange unit configured to load a test sample on a solid-phase cartridge S having a strong ion-exchange resin phase, to allow the strong ion-exchange resin phase to adsorb a predetermined organic compound, then supply a dehydration solvent to dehydrate the strong ion-exchange resin phase, and a derivatization unit configured to feed a predetermined amount of the derivatization reagent to the dehydrated strong ion-exchange resin phase to allow the derivatization reagent to retain for a predetermined time period, thereby trimethylsilylating the organic compound adsorbed on the strong ion-exchange resin phase, and simultaneously desorbing the trimethylsilylated organic compound from the strong ion-exchange resin phase, and then supply a push-out solvent to push the trimethylsilylated organic compound desorbed, out of the solid-phase cartridge S. The device enables at least one organic compound selected from amino acids, organic acids and glucides contained in a test sample to be derivatized and collected easily in a short period of time, and automation of the pre-analysis treatment.
G01N 30/96 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography using ion-exchange
An analysis preprocessing method with which it is possible to obtain an analysis sample in which the concentrations of multiple constituents, which are included in said sample in significantly different concentrations, have been adjusted to predetermined ranges, said method comprising: a first holding step in which predetermined amounts of a sample and a first solvent are supplied to a solid phase, and a low-concentration constituent is held in the solid phase; a first washing step in which the first solvent is supplied to the solid phase, and a high-concentration constituent is removed from the solid phase; a second holding step in which the sample and a second solvent are supplied to the solid phase in smaller amounts than at the first holding step, and the high-concentration constituent is held in the solid phase; and an eluting step in which the constituents held in the solid phase are collectively extracted by an extraction solvent. The first solvent can dissolve the low-concentration constituent and the high-concentration constituent, and has a property for holding the low-concentration constituent in the solid phase and not holding the high-concentration constituent. The second solvent has a property for holding the low-concentration constituent and the high-concentration constituent in the solid phase.
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
G01N 30/88 - Integrated analysis systems specially adapted therefor, not covered by a single one of groups
G01N 30/96 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography using ion-exchange
[Problem] To provide a solid-phase extraction cartridge whereby liquid pressure piping and gas pressure piping can be connected directly to a sample introduction part, and a solvent or a sample can be supplied to the sample introduction part of the solid-phase extraction cartridge using a chip or the like and mixed. [Solution] A solid-phase extraction cartridge 1 in which a lower frit 3 is mounted inside a cylindrical cartridge body 2, a solid-phase filler 4 is packed thereon, and an upper frit 5 is furthermore pressed thereon as a plug, wherein a tapered upper connecting part 6A and lower connecting part 6B decreasing in diameter progressively toward a discharge side are formed at the top and bottom of the inner surface of a sample introduction part 6 of the cartridge body 2, the minimum inside diameter DA of the upper connecting part 6A is formed so as to be larger than the maximum inside diameter DB of the lower connecting part 6B, gas pressure piping inserted in the sample introduction part 6 is connected to the upper connecting part 6A so as to be in close contact therewith, and liquid pressure piping inserted in the sample introduction part 6 is connected to the lower connecting part 6B so as to be in close contact therewith.
G01N 1/10 - Devices for withdrawing samples in the liquid or fluent state
G01N 30/00 - Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
[Problem] To provide a dry ice freezer and crusher from which homogenized pulverized samples can be obtained more efficiently. [Solution] A dry ice freezer and crusher formed from: a crushing container (1) which is provided with a container main body (3) constituted of a bottom plate (5) and a side plate (6) standing up from the peripheral edge of the bottom plate (5), having an upper surface opening (B), and provided internally with a pulverizing blade (C) and is provided with a cover body (4) which closes off the upper surface opening (B) and in which a gas release hole (7) is formed; and a crusher main body (2) which is provided with a drive device (D) for rotating the pulverizing blade (C). The side plate (6) of the container main body (3) is formed by a vacuum double-wall structure by vacuum evacuating an internal space (S1) formed by metal plates (P1A, P1B) that face each other. A radiant heat transfer prevention body (Q1) is provided that serves as a mirror in the internal space (S1). The insulating properties of the container main body (3) are extremely high; therefore, the inside surface temperature for the side plate (6) is not increased by the effects of ambient temperature when crushing a sample at room temperature, so no part of the sample adheres to the inside surface of the side plate (6) during crushing.
B02C 18/12 - Disintegrating by knives or other cutting or tearing members which chop material into fragmentsMincing machines or similar apparatus using worms or the like with rotating knives within vertical containers with drive arranged below container
B02C 18/16 - Disintegrating by knives or other cutting or tearing members which chop material into fragmentsMincing machines or similar apparatus using worms or the like with rotating knives Details
14.
AMINO ACID, ORGANIC ACID, AND SUGAR ANALYSIS PRETREATMENT DEVICE AND ANALYSIS PRETREATMENT METHOD
This invention makes it possible to easily and quickly derivatize and recover at least one organic compound selected from amino acids, organic acids, and sugars included in a sample to be analyzed, by means of an amino acid, organic acid, and sugar analysis pretreatment device comprising: an ion-exchange unit configured so as to load samples to be analyzed into a solid-phase cartridge S having a strong ion-exchange resin phase, adsorb a prescribed organic compound to the strong ion-exchange resin phase, and then supply a dehydrating solvent to dehydrate the strong ion-exchange resin phase; and a derivatization unit configured so as to supply a prescribed amount of a derivatization reagent to the dehydrated strong ion-exchange resin phase and retain the derivatization reagent for a prescribed period of time, desorb the organic compound adsorbed to the strong ion-exchange resin phase from the strong ion-exchange resin phase and at the same time trimethylsilylate the organic compound, and then supply an extrusion solvent to extrude the desorbed trimethylsilylated organic compound from the solid-phase cartridge S. This invention also makes automation possible.
[Problem] To provide a solid phase extraction cartridge that is provided with a discharge tip at a cartridge main body, is such that a tube can be connected without using a connection adaptor, and is favorable for using only a small amount of filling, thus having high analytic accuracy. [Solution] The solid phase extraction cartridge (1) results from a sample outflow-side frit (3) being mounted within a tubular cartridge main body (2), a solid phase filler (4) filling above same, a sample inflow-side frit (5) being abutted above same, and the result being stoppered, a tubular discharge tip (6) being provided below the sample outflow-side frit (3) in the cartridge main body (2). The inner surface (7B) of the discharge tip (6) is formed in a tapered shape that narrows in diameter in the upward direction, and a tube (PO) inserted from below into the discharge tip (6) makes tight contact at the top of the inner surface (7B). It is possible to easily and reliably connect the tube (PO) without using a connection adaptor, dead space is greatly reduced, and an eluate flows smoothly without waste, and so analytic accuracy increases.
A combination of a liquid chromatograph and a gas chromatograph was a possible measure for improving precision in analyzing organic chemical substances. However, because elutes from liquid chromatoghaphies contain water and a highly polar solvent, injection thereof into a gas chromatograph has been impossible. Consequently, to develop an analytical method which realizes that combination and an apparatus thereof has been a subject. The method of the quantitative analysis comprises subjecting a sample for analysis prepared beforehand by extracting organic chemical substances from an assay sample to fractionation by a liquid chromatograph, continuously adsorbing a fractionated elute containing a substance to be determined onto a solid-phase cartridge while conducting the fractionation, eluting this substance, which has been adsorbed on the solid-phase cartridge, with an eluent, and transferring the elute to a storage chamber of a gas chromatograph.
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