Abstract

The technical field of active substance preparations is belonged to, and provided are astaxanthin pickering emulsion with colon targeted delivery function, and a preparation method and application method thereof. The preparation method includes the following steps: S1, reparation of deacetylated-oxidized chitin nanofiber (DE-TO-ChNF)/carboxymethyl konjac glucomannan (CMKGM) nanogel; S2, preparation of astaxanthin oil phase; and S3, preparation astaxanthin pickering emulsion. Natural polysaccharide derivatives DE-TO-ChNF and CMKGM are used as raw materials, and stable crosslinking between covalent bonds and non-covalent bonds are formed between molecules in a composite system through the NHS/EDC, so as to form a stable nanogel. Then the nanogel is used as emulsifier and flaxseed oil rich in astaxanthin is used as oil phase to prepare the astaxanthin pickering emulsion by homogenization.

IPC Classes  ?

• A61K 9/107 - Emulsions
• A61K 31/122 - Ketones having the oxygen atom directly attached to a ring, e.g. quinones, vitamin K1, anthralin
• A61K 47/18 - AminesAmidesUreasQuaternary ammonium compoundsAmino acidsOligopeptides having up to five amino acids
• A61K 47/36 - PolysaccharidesDerivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• A61K 47/46 - Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts

Abstract

A SEC12-like protein gene CPU1 and application thereof in improving soybean phosphorus efficiency are disclosed. Through genome-wide association studies, a major genetic locus affecting soybean phosphorus efficiency is identified, and the candidate gene CPU1 is discovered and validated. There are natural variations in gene CPU1 in soybean population, including two alleles, phosphorus-inefficient allele CPU1-H1 and phosphorus-efficient allele CPU1-H2. Studies based on CPU1-transformation plants shows that inhibiting the expression of the allele CPU1-H2 significantly reduces soybean phosphorus efficiency, and ultimately reduces the biomass and yield of transgenic plants. The present disclosure provides new scientific insights into genetic bases underlying natural phenotypic variation in crops, and provides novel allele resources for molecular breeding of phosphorus efficiency.

IPC Classes  ?

• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells

Abstract

Provided is a method for regulating differentiation of multicellular epidermal hairs of a plant. The differentiation of different types of multicellular epidermal hair cells of the plant is regulated by mutating/silencing/overexpressing one or more epidermal hair development related genes. The formation of shield-like secretory epidermal hairs of the plant can be promoted by overexpressing LFS; or knocking out/slicing one or two of Slwox3b and MX1 genes; or simultaneously knocking out/slicing Slwox3b, MX1, MTR1, MTR2, and MTR3 genes; or overexpressing WoP635R and/or WoI692RD695Y genes while knocking out/slicing Slwox3b and MX1 genes. The differentiation of finger-like multicellular epidermal hairs of the plant is promoted by knocking out/slicing LFS gene or one or more of MTR1, MTR2, MTR3 genes. The method has important significance for enhancing the stress resistance of the plant and enhancing the capability of synthesizing and secreting metabolites of the plant.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy
• A01H 6/82 - Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
• A01H 6/28 - Cannabaceae, e.g. cannabis

Abstract

A phosphorus-dissolving bacterium RBC25 regulated by Phosphate deficiency and application thereof are provided. The phosphorus-dissolving bacterium RBC25 is obtained by isolating from roots of soybean planted in acidic soil. The phosphorus-dissolving bacterium RBC25 belongs to Burkholderia sp., which has a dissolving capacity for insoluble inorganic phosphate and organic phosphate. The phosphorus-dissolving bacterium RBC25 is regulated by phosphate deficiency, which can effectively colonize on soybean roots under phosphate-deficient conditions, and thus promote soybean growth under low-phosphate conditions. The phosphorus-dissolving bacterium RBC25 releases dissolved phosphate by dissolving insoluble inorganic phosphate and organic phosphate, so that it helps plants to absorb insoluble phosphate. Compared with the non-inoculated control, inoculating RBC25 in greenhouses can increase the biomass and phosphorus content of soybean by 39.6% and 35.4%, respectively, and inoculating RBC25 in fields can increase the biomass and phosphorus content of soybean by 12.5% and 16.9%, respectively.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor

Abstract

A leather tanning method using chrome-free and formaldehyde-free adhesive pertains to the technical field of leather tanning, in which the quaternary ammonium salt cationic resin adhesive is used for leather pretanning and retanning. After drying, the leather has excellent performance, which is comparable to traditional chrome and formaldehyde tanned leather. The method solves the drawbacks of using chromium and formaldehyde tanning agents in the leather tanning industry, which pollutes the environment and raises health concerns. The present disclosure has obvious advantages in environmental protection, huge industrial application value, and has broad application prospects.

IPC Classes  ?

• C14C 3/28 - Multi-step processes
• C14C 3/18 - Chemical tanning by organic agents using polycondensation products or precursors thereof

Abstract

The present disclosure discloses a method for tomato soilless cultivation with suspended trough, including four aspects: setting of soilless cultivation substrate trough, preparation of soilless cultivation substrate, efficient and economical grafting and plant management. The efficient and economical grafting includes seedling raising, preparation of new scion, grafting and healing culture. The plant management includes planting, pruning and vine raising, flower and fruit management, and plant type maintenance. the present disclosure utilizes a soilless cultivation substrate trough, which can overcome the shortcomings of poor water retention, difficult control of temperature and water and fertilizer application in substrate bag cultivation and give full play to the characteristics of the cultivation substrate. The use of efficient and economical grafting and supporting plant management methods can optimize plants configuration, simplifying the work content of plant management, and improving the utilization rate of cultivation space.

IPC Classes  ?

• A01G 22/05 - Fruit crops, e.g. strawberries, tomatoes or cucumbers
• A01G 24/25 - Dry fruit hulls or husks, e.g. chaff or coir
• A01G 2/30 - Grafting

Abstract

Disclosed are an apparatus and method for electrochemically-assisted aerobic composting of organic solid waste. The apparatus for aerobic composting comprises a composting reactor, a working electrode, an auxiliary electrode, and a reference electrode; the working electrode is provided on the inner wall of the composting reactor; the reference electrode is provided between the working electrode and the auxiliary electrode; the bottom of the composting reactor is provided with an aeration conduit having an aeration head. Also disclosed is a method for performing electrochemically-assisted aerobic composting of organic solid waste by using the apparatus. The implementation of electrochemically-assisted composting by means of the composting apparatus of the present invention can enrich and utilize electric microorganisms, promote redox reaction in a compost body, rapidly increase a compost temperature, shorten a composting cycle, and simultaneously increase the compost maturity.

IPC Classes  ?

• C05F 17/02 - Apparatus therefor

Abstract

Disclosed in the present invention is a method for rapidly decreasing antibiotics and resistance genes in organic solid waste, which belongs to the field of organic solid waste treatment. The present invention utilizes aerobic fermentation bacteria capable of withstanding a temperature of at least 80 °C and controls the pile temperature of no lower than 80 °C for fermentation for at least 5 to 7 days so as to carry out ultra-high temperature aerobic fermentation of organic solid waste, being capable of obtaining effects of rapidly and stably decreasing antibiotics and antibiotic resistance genes in organic solid waste The ultra-high temperature aerobic fermentation not only can rapidly degrade antibiotics and ARGs, but also can change the whole microbial colony structure in the pile, kill 90% of the ARGs-bearing microorganisms (mainly, Proteobacteria and Bacteroidetes), reduce the risk of gene transfer of ARGs, and control the diffusion thereof from the source, thereby ensuring that ARGs will not rebound. Not only the treatment of organic solid waste is achieved, but also the dual effects of efficiently removing antibiotic residues and resistance gene contamination are obtained. The method of the present invention does not need external heating, and only relies on the metabolic energy of the thermophilic microorganism to reach the high temperature of fermentation, having low energy consumption and being environmentally friendly.

IPC Classes  ?

• C05F 17/00 - Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
• C12N 1/20 - BacteriaCulture media therefor

Abstract

A method for inducing multiple layers of Casparian strips in a plant root. The method induces a Casparian strip in a plant root using a plant transcription factor SHORT-ROOT (SHR) or MYB36 in combination with plant-derived small peptides CIF1 and CIF2. The constitutive (using a 35S promoter) or inductive (using an estrogen-inducible expression system) expression of SHR or MYB36 enables a plurality of genes involved in Casparian strip synthesis to be induced in cortical cells and other essential tissue cells in plant roots.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

Disclosed are an ultra-high temperature aerobic fermentation bacteria preparation prepared by using municipal sludge, and a preparation method therefor, involving: carrying out fermentation after the activation of ultra-high-temperature aerobic bacteria, removing a supernatant from a fermentation broth, and adding a protective agent and stirring same until uniform, drying same, pulverizing same by means of a pulverizer, and sieving and packaging same. A medium used fermentation is prepared using municipal sludge.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/01 - Bacteria or actinomycetales
• C05F 7/00 - Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses

Abstract

A comb foundation for reducing pesticide and insecticide residues, which is prepared, after adding an adsorption material such as activated carbon to beeswax, according to a traditional method of making a beeswax comb foundation. The obtained comb foundation can effectively adsorb pesticides and insecticides in beeswax, reducing the residual amount of pesticides and insecticides so as to maintain the normal development of larvae, and can also avoid the accumulation of residual pesticides and insecticides during the recycling of beeswax, thereby insuring and enhancing the quality of bee products

IPC Classes  ?

• A01K 47/04 - Artificial honeycombs
• A01K 47/02 - Construction or arrangement of frames for honeycombs
• A01K 47/06 - Other details of beehives, e.g. ventilating devices, entrances to hives, guards, partitions or bee escapes
• A01K 47/00 - Beehives

Abstract

Triticum aestivumTriticum aestivumTriticum aestivumTriticum aestivum resistance breeding.

IPC Classes  ?

• C12N 15/29 - Genes encoding plant proteins, e.g. thaumatin
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

The invention relates to a method of increasing yield in plants comprising increasing the expression of a nucleic acid encoding a phosphate transporter (PT7) polypeptide. The invention also relates to methods of making such plants and genetically altered plants that display an increased yield.

IPC Classes  ?

• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C07K 14/415 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from plants
• C12N 15/90 - Stable introduction of foreign DNA into chromosome
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• A01H 5/00 - Angiosperms, i.e. flowering plants, characterised by their plant partsAngiosperms characterised otherwise than by their botanic taxonomy

Abstract

A preparation method of core-shell type granular active carbon with a powdery core comprises the following steps: (1) preliminary sizing: mixing an organic binding agent aqueous solution and powder active carbon and performing extrusion molding to obtain a granular material; (2) coating: forming a uniform inorganic coating layer on the outer layer of the granular material; (3) heat treatment: causing the organic binding agent in the material to undergo thermal decomposition so that the structure of the granular material of the core is damaged to form powder and the inorganic coating layer at the outer layer forms a hard coating layer to protect the powder from leakage; and (4) screening to obtain granular active carbon products with different specifications and having a powdery core. The prepared formed active carbon integrates the advantages of powdery active carbon and granular active carbon and thus achieves relatively high adsorption performance and excellent strength.

IPC Classes  ?

• B01J 20/20 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising free carbonSolid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising carbon obtained by carbonising processes
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• B01J 20/30 - Processes for preparing, regenerating or reactivating

Abstract

The present application provides a method for breeding tea plants capable of inhibiting tumor. With the content of basic active components of the tea plants, the tea plants capable of inhibiting tumor are selected using distant hybridization according to the content of total catechins≥22%, wherein the content of EGCG≥12%, the content of catechins C≥2%, the content of ECG≥3.5%, and the content of caffeine≥4.5%. The tea produced can inhibit the growth of several kinds of human tumor cells in vitro.

IPC Classes  ?

• A01H 1/02 - Methods or apparatus for hybridisationArtificial pollination
• A01H 1/04 - Processes of selection
• A23F 3/00 - TeaTea substitutesPreparations thereof
• A61K 36/82 - Theaceae (Tea family), e.g. camellia
• A61P 35/00 - Antineoplastic agents

Abstract

An energy grass comprehensive recycling method, consisting of five steps: preparing methane with energy grass, producing edible fungi with the methane residue, producing fungi residue fodder with the fungi residue, feeding livestock and poultry with the fungi residue fodder, producing organic fertilizer with the manure of the livestock and poultry. The method produces five types of commodities, methane, edible fungi, fodder, livestock and poultry and organic fertilizer, realizing efficient utilization of resources and building an efficient agricultural comprehensive recycling system.

IPC Classes  ?

• A01G 1/04 - Cultivation of mushrooms (composts or fertilisers for cultivating mushrooms C05)
• B09B 3/00 - Destroying solid waste or transforming solid waste into something useful or harmless
• C05F 3/00 - Fertilisers from human or animal excrements, e.g. manure
• C12P 5/02 - Preparation of hydrocarbons acyclic
• A23K 1/00 - Animal feeding-stuffs
• A01K 67/02 - Breeding vertebrates

Abstract

The invention relates to a mosquito-killing Bacillus thuringiensis suspended slow release formulation and a preparation method thereof. According to the preparation method of the present invention, using sodium alginate as a carrier and calcium chloride as a crosslinking agent, a mosquito-killing Bacillus thuringiensis raw powder is added as an effective active ingredient, and the slow release formulation has a high-efficiency mosquito-killing activity; the formulation has a suspended slow release effect via hollow glass beads, thus resisting ultraviolet radiation and prolonging the longevity of residues. The formulation has a reasonable formula, high insecticidal toxicity, good control effect, strong ultraviolet radiation resistance and long longevity of residues in the wild. The experiment indicates that the product of the invention has a good effect on killing mosquito larvae in water, and as the treatment time is prolonged, the average relative death rate of test insects is gradually increased until the relative death rate of test insects within 192 hours reaches 100%; and the product of the invention has a high mechanical property and long-acting suspension property, and the suspension begins to precipitate at 144 hours, and the suspension rate is kept to be 58.3% for 192 hours.

IPC Classes  ?

• A01N 65/12 - Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
• A01N 25/22 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
• A01N 25/04 - Dispersions or gels
• A01N 25/28 - Microcapsules
• A01P 7/04 - Insecticides
• A01N 63/00 - Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates

Abstract

Provided in the present invention are Bt toxin Cry1Ab-loop2-P2S having high toxicity against Nilaparvata lungens and an engineered bacterium. With Bravo model, a Bt Cry toxin effect model, serving as the theoretical basis, the present invention utilizes a bacteriophage peptide library display technology to screen peptide P2S that is capable of bonding with the midgut of Nilaparvata lungens; furthermore, a loop2 sequence of Domain II of Cry molecule is replaced in the sequence of the peptide by utilizing a molecular fragment replacement method, the cry1Ab-loop2-p2s toxin gene is constructed, the gene and universal expression vector pGEX-KG are connected and transformed into Escherichia coli BL21-DE2, and, after stability measurement, bonding activity measurement, and toxicity measurement, engineered bacteria 1Ab-P2S (CGMCC No: 6426) that is capable of expressing the Cry1Ab-loop2-P2S toxin having high toxic effects against Nilaparvata lungens is acquired.

IPC Classes  ?

• C12N 15/32 - Bacillus crystal proteins
• C07K 7/06 - Linear peptides containing only normal peptide links having 5 to 11 amino acids
• C12N 15/62 - DNA sequences coding for fusion proteins
• C07K 19/00 - Hybrid peptides
• A01P 7/04 - Insecticides
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12R 1/19 - Escherichia coli

Abstract

Disclosed is a stevia tea rich in steviol glycosides and a processing method thereof. The method comprises the following steps: the fresh stevia leaves are spread out for airing, subjected to a deactivation of enzymes, rolled and baked to dry. The stevia tea made by the method has a pleasant aroma, mellow taste, high steviol glycoside content, and the stevioside, rebaudioside A and total glycosides thereof is respectively 42.40%, 31.21% and 32.71% higher than that obtained by air drying when compared with the commercially available stevia leaves that have been dried in the sun.

IPC Classes  ?

• A23F 3/34 - Tea substitutes, e.g. mateExtracts or infusions thereof

Abstract

Disclosed in the present invention are a method for releasing large amount of negative ions by indoor plant and device thereof. The method utilizes a high-voltage pulse electric field to stimulate the soil around roots of crassulacean acid metabolism plant to promote the releasing of negative ions quickly and efficiently and enhance the concentration of negative ions released by indoor plants. The method can regulate human physiology, and improve metabolism, immunity and health.

IPC Classes  ?

• A61L 9/22 - Ionisation
• A01G 7/04 - Electric or magnetic treatment of plants for promoting growth

Abstract

An ultra low density composite prepared by a novel liquid foaming process using natural fibres is described. The said process results in a three-dimensional reticulated structure with natural fibres using the hydrogen bond of the hydroxyl group generated within the fibres and/or introducing external copolymers to the bonded "knot" or "joint" at the end of the fibres. The degree of branched end in the fibres and their aspect ratio define the density of the composite. The ultra low density composite has a density of 10-120 kg/m3 and can be used as impact resistant materials for packaging, wall panels and insulation materials for buildings and industrial heat conservation materials.

IPC Classes  ?

• C08J 9/35 - Composite foams, i.e. continuous macromolecular foams containing discontinuous cellular particles or fragments