Abstract

Described is a method and component parts of a recombinant expression system for the expression of a biological molecule. In particular, an isolated vesicle nucleating polypeptide (VNp) comprising an amphipathic alpha helix polypeptide derived from the amino terminus of synuclein isoforms and variants thereof is used to promote, enhance or increase the formation of vesicles in the expression system to enable purification of the biological molecule.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Goods & Services

Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; process development (term considered too vague by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); development of processes for the use in the manufacture of biotechnology products; project studies (term considered too vague by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparations of biological samples for analysis in research laboratories (term considered linguistically incorrect by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparations of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries; biological preparations for use in science; biological preparations for use in cell cultures; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; custom processing and manufacturing services in the field of biopharmaceuticals; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research, development and analysis services; development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; biotechnology process development; development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; technical project studies; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparation of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Chemicals used in industry and science; cell cultures used in industry and science; biological preparations for use in science; biological preparations for use in cell cultures; proteins for use in industry, enzymes; microbial preparations being chemicals for industry; cultures of micro-organisms other than for medical or veterinary use; chemical reagents; organic chemicals for use in science, namely genes being DNA sequences; plasmids being small independently replicating pieces of DNA and vectors being small independently replicating pieces of DNA that can transfer DNA sequences, being chemicals for use in science; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of chemicals and proteins; custom manufacture of chemicals and proteins for use in biotechnology and pharmaceuticals; manufacture, processing and production of scientific and industrial products to the order and specification of others; custom processing and manufacturing services; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research; scientific analysis; technological analysis services; scientific research and development; industrial process development; industrial design services; provision of facilities for scientific, industrial and technical research and development; research and development of scientific, industrial and technical products; technical project studies; testing of scientific, industrial and technical products; scientific, industrial and technical research, development and analysis services in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries; biological preparations for use in science; biological preparations for use in cell cultures; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; custom processing and manufacturing services; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research, development and analysis services; development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; industrial process development; development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; technical project studies; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparation of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries; biological preparations for use in science; biological preparations for use in cell cultures; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; custom processing and manufacturing services; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research, development and analysis services; development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; industrial process development; development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; technical project studies; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Chemicals used in industry and science; cell cultures used in industry and science; biological preparations for use in science; biological preparations for use in cell cultures; proteins for use in industry, enzymes; microbial preparations being chemicals for industry; cultures of micro-organisms other than for medical or veterinary use; chemical reagents; organic chemicals for use in science, namely genes being DNA sequences; plasmids being small independently replicating pieces of DNA and vectors being small independently replicating pieces of DNA that can transfer DNA sequences, being chemicals for use in science; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of chemicals and proteins; custom manufacture of chemicals and proteins for use in biotechnology and pharmaceuticals; manufacture, processing and production of scientific and industrial products to the order and specification of others; custom processing and manufacturing services; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research; scientific analysis; technological analysis services; scientific research and development; industrial process development; industrial design services; provision of facilities for scientific, industrial and technical research and development; research and development of scientific, industrial and technical products; technical project studies; testing of scientific, industrial and technical products; scientific, industrial and technical research, development and analysis services in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparation of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries; biological preparations for use in science; biological preparations for use in cell cultures; cell culture reagents for scientific and research use; media for cell culture for use in the biotechnological industry; chemical products for biotechnological purposes; chemicals for use in biotechnological manufacturing processes. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; custom processing and manufacturing services in the field of biopharmaceuticals; custom processing and manufacturing of biological products; custom manufacture of biopharmaceuticals; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Scientific, industrial and technical research, development and analysis services; development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; biotechnology process development; development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; technical project studies; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; design and development of manufacturing processes in the field of biopharmaceuticals; development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; preparation of biological samples for analysis in research laboratories; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; process development (term considered too vague by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); development of processes for the use in the manufacture of biotechnology products; project studies (term considered too vague by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparations of biological samples for analysis in research laboratories (term considered linguistically incorrect by the International Bureau pursuant to Rule 13 (2) (b) of the Regulations); designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparation of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Goods & Services

Custom processing and manufacturing services; custom processing and manufacturing of pharmaceuticals; custom manufacture of pharmaceuticals; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others; cell culture manufacturing services for others; custom manufacture of biologics; information, advice and consultancy in relation to all the aforesaid. Construction services; building services; construction of pharmaceutical facilities; onsite management relating to the construction of pharmaceutical manufacturing facilities; building and construction of factories; custom construction of factories and pharmaceutical manufacturing facilities; information, advice and consultancy in relation to all the aforesaid. Scientific and technological services; research and development; scientific, industrial and technical research, development and analysis services; scientific, industrial and technical research, development and analysis services in the field of biotechnology; industrial process development; development of processes for the use in the manufacture of biotechnology products; technical project studies; biotechnology testing; research and consultancy in the field of biotechnology; research and development in the pharmaceutical and biotechnology industry; scientific, technological and pharmaceutical process development and design; biopharmaceutical product development; software development, programming and implementation; design and development of software; design and development of manufacturing processes in the field of biopharmaceuticals; design of manufacturing methods; design of industrial buildings; design of manufacturing plants; engineering services; research laboratories; preparations of biological samples for analysis in research laboratories; designs services relating to process systems for the biotechnology industry; design services relating to plant for the biotechnology industry; information, advice and consultancy in relation to all the aforesaid.

Abstract

Described is a method and component parts of a recombinant expression system for the expression of a biological molecule. In particular, an isolated vesicle nucleating polypeptide (VNp) comprising an amphipathic alpha helix polypeptide derived from the amino terminus of synuclein isoforms and variants thereof is used to promote, enhance or increase the formation of vesicles in the expression system to enable purification of the biological molecule.

IPC Classes  ?

• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• A61K 9/50 - Microcapsules
• C12N 9/50 - Proteinases
• C12N 15/67 - General methods for enhancing the expression
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

Described is a method and component parts of a recombinant expression system for the expression of a biological molecule. In particular, an isolated vesicle nucleating polypeptide (VNp) comprising an amphipathic alpha helix polypeptide derived from the amino terminus of synuclein isoforms and variants thereof is used to promote, enhance or increase the formation of vesicles in the expression system to enable purification of the biological molecule.

IPC Classes  ?

• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals

Abstract

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• B01D 61/14 - UltrafiltrationMicrofiltration
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
• C07K 1/16 - ExtractionSeparationPurification by chromatography

Abstract

A process for purifying a liquid feedstock comprising a monoclonal antibody and impurities, the process comprising passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified monoclonal antibody and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v) which include a multiple inlet flow-controller comprising two or more variable flow inlet valves for in situ production of a bioprocessing liquid by combining at least two liquids in a desired ratio. One of the units performs chromatography and another performs viral inactivation. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• C07K 1/22 - Affinity chromatography or related techniques based upon selective absorption processes
• C07K 1/18 - Ion-exchange chromatography
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• B01D 15/14 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• B01D 61/14 - UltrafiltrationMicrofiltration
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion

Abstract

A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Goods & Services

Continuous production, including continuous manufacture and purification, of biological products such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells, to the order and specification of others; continuous expression, isolation and purification, of biological products such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells, to the order and specification of others; continuous connected upstream and downstream production of biological products such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells, to the order and specification of others. Scientific, industrial and technical research, development and analysis services related to continuous production, including continuous manufacture and purification, of biological products such as such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells; process development related to the continuous production, including continuous manufacture and purification, of biological products such as such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells; industrial design services related to the continuous production, including continuous manufacture and purification, of biological products such as such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells; provision of facilities for scientific, industrial and technical research development related to the continuous production, including continuous manufacture and purification, of biological products such as such as cells, viruses and biomolecules, including genes and proteins, obtained from recombinant cells.

Abstract

A static mixing apparatus for mixing a fluid, preferably a liquid is provided. The mixer comprises a plurality of chambers (5, 8, 12, 15) in series, the first chamber (5) of the series comprising a fluid inlet (4), and the final chamber (15) of the series comprising a fluid outlet (16), each chamber other than the final chamber in the series being in fluid connection with a subsequent chamber, the fluid connection comprising a plurality of orifices (7, 10, 11, 14) dispersed along a direction of flow, the nearest point to the fluid inlet of each subsequent orifice to the inlet overlapping with the furthest point from the fluid inlet of the previous orifice, and being off-set along the direction of flow from the previous orifice.

IPC Classes  ?

• B01F 5/06 - Mixers in which the components are pressed together through slits, orifices, or screens

Abstract

A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.

IPC Classes  ?

• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• C07K 1/14 - ExtractionSeparationPurification
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

in situin situ production of a bioprocessing liquid by combining at least two liquids in a desired ratio. One of the units performs chromatography and another performs viral inactivation. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• B01D 15/36 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
• B01D 15/38 - Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups , e.g. affinity, ligand exchange or chiral chromatography
• C07K 1/16 - ExtractionSeparationPurification by chromatography
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C12N 7/04 - Inactivation or attenuationProducing viral sub-units
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/46 - Flow patterns using more than one column
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier

Abstract

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

IPC Classes  ?

• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/46 - Flow patterns using more than one column
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• C12M 1/00 - Apparatus for enzymology or microbiology
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier

Abstract

Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided. The apparatus comprises at least a first and a second means for carrying out a unit operation, each means for carrying out a unit operation comprising a feed for a liquid in fluid connection with the inlet of a flow-controller comprising a variable flow inlet valve and an outlet, wherein at least one of the means for carrying out a unit operation comprises feeds for at least two liquids, the feeds being in fluid connection with the inlets of a multiple inlet flow-controller comprising two or more variable flow inlet valves for dosing the at least two liquids, the flow-controller also comprising an outlet; a feed for a liquid feedstock comprising the target substance in fluid connection with the outlet from the flow-controller thereby to enable combination of the feed for a liquid comprising the target substance with the mixed bioprocessing liquids to produce a device feed; a device for achieving a processing operation comprising a device inlet and a device outlet, the device inlet being in fluid connection with the device feed; and a means for imparting flow through the flow controller and from the feed for the liquid feedstock through the processing device via the device inlet; and wherein the feed for a liquid feedstock comprising the target substance for the second means for carrying out a unit operation comprises the outlet from the first means for carrying out a unit operation.

IPC Classes  ?

• G01N 30/46 - Flow patterns using more than one column
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient

Goods & Services

Apparatus for use in separation and purification, including separation and purification of biological products such as cells, viruses and biomolecules, including genes and proteins; liquid chromatography apparatus for laboratory or scientific use; ion exchange apparatus for laboratory or scientific use; ultrafiltration apparatus for laboratory or scientific use; diafiltration apparatus for laboratory or scientific use; apparatus for diluting or concentrating biological products for laboratory or scientific use; apparatus for removal of viruses from solutions, including biological products, for laboratory or scientific use; apparatus for inactivation of viruses from solutions for laboratory or scientific use; apparatus for membrane filtration for laboratory or scientific use; apparatus for performing chromatographic separations and parts thereof for laboratory or scientific use; flowpaths, parts and fittings, including tubing, valves, in-line mixers, pressure sensors, temperature sensors and/or other sensors for all the aforesaid goods; computer software for control of the aforesaid goods; computer software for management of biological product purification processes. Apparatus for use in separation and purification for industrial purposes, including separation and purification of biological products such as cells, viruses and biomolecules, including genes and proteins; liquid chromatography apparatus for industrial purposes; ion exchange apparatus for industrial purposes; ultrafiltration apparatus for industrial purposes; diafiltration apparatus for industrial purposes; apparatus for diluting or concentrating biological products for industrial purposes; apparatus for removal of viruses from solutions, including biological products for industrial purposes; apparatus for inactivation of viruses from solutions for industrial purposes; apparatus for membrane filtration for industrial purposes; apparatus for performing chromatographic separations and parts thereof for industrial purposes; flowpaths, parts and fittings, including tubing, valves, in-line mixers, pressure sensors, temperature sensors and/or other sensors for all the aforesaid goods. Processing and manufacturing of biological products such as cells, viruses and biomolecules, including genes and proteins, including to the order and specification of others; manufacture, processing and production of biomolecules such as cells, viruses and biomolecules, including genes and proteins, to the order and specification of others. Scientific, industrial and technical research, development and analysis services related to biological products such as such as cells, viruses and biomolecules, including genes and proteins; process development related to the manufacture and purification of biological products such as such as cells, viruses and biomolecules, including genes and proteins; industrial design services related to the manufacture and purification of biological products such as such as cells, viruses and biomolecules, including genes and proteins; provision of facilities for scientific, industrial and technical research development related to the manufacture and purification of biological products such as such as cells, viruses and biomolecules, including genes and proteins.

Abstract

A protein expression system for use in a prokaryotic host is provided, the expression system comprising: a) an expression cassette comprising a nucleic acid sequence encoding a protein of interest operably linked to a T7 RNA polymerase-dependent promoter; and b) an expression cassette comprising a nucleic acid sequence encoding T7 RNA polymerase operably linked to a host polymerase-dependent λ phage promoter and a single perfect palindrome operator sequence; wherein the expression cassette for T7 RNA polymerase is located on the chromosome of a host cell.

IPC Classes  ?

• C12N 15/73 - Expression systems using phage lambda regulatory sequences
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon

Goods & Services

Continuous, custom production or manufacture, namely continuous extraction, and purification, of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells, to the order and specification of others; Continuous, custom manufacture, namely continuous expression isolation, and purification, of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells, to the order and specification of others; Continuous, custom production or manufacture, namely, continuous connected upstream and downstream manufacture of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells, to the order and specification of others Scientific, industrial and technical research, development and analysis services related to continuous production, continuous manufacture and purification, of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells; Scientific research and development, namely process development related to the continuous production, continuous manufacture and purification, of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells; Industrial design services related to the continuous production, continuous manufacture and purification, of biological in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells; Provision of facilities for scientific, industrial and technical research development related to the continuous production, continuous manufacture and purification, of biological products in the nature of cells, viruses and biomolecules, genes and proteins, obtained from recombinant cells

Abstract

Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means for mixing at least two liquids comprising a multiple inlet flow-controller, the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device configured in single-pass mode.

IPC Classes  ?

• B01D 61/18 - Apparatus therefor
• B01D 61/20 - AccessoriesAuxiliary operations
• C07K 1/14 - ExtractionSeparationPurification
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01D 61/14 - UltrafiltrationMicrofiltration
• B01D 61/16 - Feed pretreatment
• B01D 61/22 - Controlling or regulating

Abstract

A method for preparing an aqueous solution of a defined pH comprising an acid, a base and optionally one or more additives is provided. The method comprises the steps of: a) calculating the theoretical concentrations of acid and base for the solution to have the defined pH using the Henderson-Hasselbach equation in combination with the Debye Huckel theory for a range of different additive concentrations; b) preparing a sample of the buffer for the range of additive concentrations and measuring the actual pH for each additive concentration; c) calculating a value for delta pH, ΔpH, being the difference between the theoretical pH and the actual pH, for each additive concentration; d) generating a mathematical model describing the relationship of ΔpH with additive concentration; e) selecting the defined pH and additive concentrations; f) using the mathematical model generated in step d) to calculate ΔpH for the defined pH and additive concentration; g) calculating a ΔpH-corrected pH by summing the defined pH and delta pH; h) using the ΔpH-corrected pH to calculate the concentrations of acid and base using the Henderson-Hasselbach equation in combination with the Debye Huckel theory; i) preparing the solution using the concentrations calculated in step h).

IPC Classes  ?

• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G05D 21/02 - Control of chemical or physico-chemical variables, e.g. pH-value characterised by the use of electric means
• B01F 25/42 - Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
• B01F 35/221 - Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
• B01F 35/71 - Feed mechanisms

Goods & Services

Fluid processing system for use in the separation and purification of biological products such as cells, viruses and biomolecules, including nucleic acids and proteins, for bioprocessing and pharmaceutical research, comprised primarily of chromatography apparatus for laboratory use, laboratory filters, and fluid handling devices used for bioprocessing applications and component parts and fittings; Liquid chromatography apparatus for laboratory use; Ion exchange chromatography apparatus for the treatment of fluids for laboratory use; Ultrafiltration apparatus for laboratory or scientific research use, namely, ultrafiltration laboratory filters and laboratory ultrafiltration filter membranes as part of scientific apparatus; Diafiltration apparatus for laboratory or scientific research use, namely, laboratory diafiltration filters; Scientific, laboratory, and measuring apparatus and instruments for diluting or concentrating biological products for laboratory or scientific research use, namely, tangential flow filtration devices for preparation of biological samples for use in the biopharmaceutical industry; Laboratory filters in the nature of viral filtration apparatus for removal of viruses from solutions for laboratory or scientific research use; Membrane filtration apparatus in the nature of membrane laboratory filters for use in laboratory equipment in the biopharmaceutical industry for treating biological fluids and solutions; Chromatography apparatus and component parts thereof for laboratory or scientific research use; Parts and fittings, namely, tubing, valves, in-line mixers, pressure sensors, temperature sensors and electronic sensors specially adapted for use with all of the aforementioned goods; Computer software for control of the aforesaid goods, namely, downloadable and recorded software for operating a fluid processing apparatus for laboratory or scientific research use comprising chromatography, membrane filtration, tangential flow filtration, viral filtration, ultrafiltration, diafiltration, viral filtration, viral inactivation, in-line conditioning, and buffer management components and systems; Computer software for management of biological product purification processes, namely, downloadable and recorded software for managing the purification of cells, viruses, and biomolecules in the nature of nucleic acids and proteins Fluid processing system apparatus and system for use in the separation and purification of biological products such as cells, viruses and biomolecules, including nucleic acids and proteins, for the pharmaceutical and biomanufacturing industries, namely, chromatography apparatus for industrial use and apparatus and instruments for use in the purification, conditioning, separation and sterilization of liquids and gases used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Liquid chromatography apparatus for industrial purposes; Ion exchange apparatus for the treatment of fluids for industrial purposes; Ultrafiltration apparatus for industrial purposes, namely, apparatus and instruments for use in the purification, separation and sterilization of liquids and gases used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Diafiltration apparatus, namely, aqueous solution filtering systems and components and biological fluid filtering systems and components for industrial purposes being apparatus and instruments for use in the purification, separation and sterilization of liquids used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Apparatus for diluting or concentrating biological products for industrial purposes, namely, tangential flow filtration apparatus being apparatus for use in the purification, separation and sterilization of liquids and gases used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Viral filtration apparatus for removal of viruses from solutions for industrial purposes, namely, apparatus and instruments for use in the purification, separation and sterilization of liquids and gases used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Virus inactivation and disinfection apparatus for the treatment of biological products in the field of biomanufacturing and pharmaceuticals, all for industrial purposes in the nature of apparatus and instruments for use in the purification, separation and sterilization of liquids and gases used in the biotechnology, pharmaceutical, medical, and biopharmaceutical industries; Membrane filtration apparatus for industrial purposes in the nature of membrane filter cartridges for the separation of biomolecules in biopharmaceutical production; Chromatography apparatus for industrial purposes and component parts thereof; Parts and fittings, namely, tubing, valves, in-line mixers, pressure sensors, temperature sensors and electric sensors, specially adapted for use with the aforementioned Processing and manufacturing of biological products such as cells, viruses and biomolecules, including genes and proteins, namely, manufacturing of pharmaceuticals using biological organisms in the manufacturing process to the order and specification of others; Manufacture of biomolecules such as cells, viruses and biomolecules, including genes and proteins, to the order and specification of others Scientific research, industrial biomedical research and technical product research and development services in the field of biomanufacturing, bioprocessing and pharmaceuticals related to biological products such as cells, viruses and biomolecules, including nucleic acids and proteins; Process development services related to the manufacture and purification of biological products such as such as cells, viruses and biomolecules, including nucleic acids and proteins, namely, biomedical and pharmaceutical research; Industrial design services related to the manufacture and purification of biological products such as cells, viruses and biomolecules, including nucleic acids and proteins; Provision of facilities for scientific, industrial and technical research and development in the field of biomanufacturing, bioprocessing and pharmaceuticals, namely, scientific laboratory services

Abstract

A static mixing apparatus for mixing a fluid, preferably a liquid is provided. The mixer comprises a plurality of chambers (5, 8, 12, 15) in series, the first chamber (5) of the series comprising a fluid inlet (4), and the final chamber (15) of the series comprising a fluid outlet (16), each chamber other than the final chamber in the series being in fluid connection with a subsequent chamber, the fluid connection comprising a plurality of orifices (7, 10, 11, 14) dispersed along a direction of flow, the nearest point to the fluid inlet of each subsequent orifice to the inlet overlapping with the furthest point from the fluid inlet of the previous orifice, and being off-set along the direction of flow from the previous orifice.

IPC Classes  ?

• B01F 5/06 - Mixers in which the components are pressed together through slits, orifices, or screens

Abstract

Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided. The apparatus comprises at least a first and a second means for carrying out a unit operation, each means for carrying out a unit operation comprising a feed for a liquid in fluid connection with the inlet of a flow-controller comprising a variable flow inlet valve and an outlet, wherein at least one of the means for carrying out a unit operation comprises feeds for at least two liquids, the feeds being in fluid connection with the inlets of a multiple inlet flow-controller comprising two or more variable flow inlet valves for dosing the at least two liquids, the flow-controller also comprising an outlet; a feed for a liquid feedstock comprising the target substance in fluid connection with the outlet from the flow-controller thereby to enable combination of the feed for a liquid comprising the target substance with the mixed bioprocessing liquids to produce a device feed; a device for achieving a processing operation comprising a device inlet and a device outlet, the device inlet being in fluid connection with the device feed; and a means for imparting flow through the flow controller and from the feed for the liquid feedstock through the processing device via the device inlet; and wherein the feed for a liquid feedstock comprising the target substance for the second means for carrying out a unit operation comprises the outlet from the first means for carrying out a unit operation.

IPC Classes  ?

• C07K 1/16 - ExtractionSeparationPurification by chromatography
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G01N 30/46 - Flow patterns using more than one column
• B01D 61/14 - UltrafiltrationMicrofiltration
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

Apparatus for processing a liquid comprising a target substance, preferably a biomolecule, is provided. The apparatus comprises at least a first and a second means for carrying out a unit operation, each means for carrying out a unit operation comprising a feed for a liquid in fluid connection with the inlet of a flow-controller comprising a variable flow inlet valve and an outlet, wherein at least one of the means for carrying out a unit operation comprises feeds for at least two liquids, the feeds being in fluid connection with the inlets of a multiple inlet flow-controller comprising two or more variable flow inlet valves for dosing the at least two liquids, the flow-controller also comprising an outlet; a feed for a liquid feedstock comprising the target substance in fluid connection with the outlet from the flow-controller thereby to enable combination of the feed for a liquid comprising the target substance with the mixed bioprocessing liquids to produce a device feed; a device for achieving a processing operation comprising a device inlet and a device outlet, the device inlet being in fluid connection with the device feed; and a means for imparting flow through the flow controller and from the feed for the liquid feedstock through the processing device via the device inlet; and wherein the feed for a liquid feedstock comprising the target substance for the second means for carrying out a unit operation comprises the outlet from the first means for carrying out a unit operation.

IPC Classes  ?

• C07K 1/16 - ExtractionSeparationPurification by chromatography
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 30/46 - Flow patterns using more than one column
• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• B01D 61/14 - UltrafiltrationMicrofiltration

Abstract

A method for preparing an aqueous solution of a defined pH comprising an acid, a base and optionally one or more additives is provided. The method comprises the steps of: a) calculating the theoretical concentrations of acid and base for the solution to have the defined pH using the Henderson-Hasselbach equation in combination with the Debye Huckel theory for a range of different additive concentrations; b) preparing a sample of the buffer for the range of additive concentrations and measuring the actual pH for each additive concentration; c) calculating a value for delta pH, ?pH, being the difference between the theoretical pH and the actual pH, for each additive concentration; d) generating a mathematical model describing the relationship of ?pH with additive concentration; e) selecting the defined pH and additive concentrations;f) using the mathematical model generated in step d) to calculate ?pH for the defined pH and additive concentration; g) calculating a ?pH-corrected pH by summing the defined pH and delta pH; h) using the ?pH-corrected pH to calculate the concentrations of acid and base using the Henderson-Hasselbach equation in combination with the Debye Huckel theory; i) preparing the solution using the concentrations calculated in step h).

IPC Classes  ?

• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• B01J 47/15 - Controlling or regulating for obtaining a solution having a fixed pH
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
• G05D 21/02 - Control of chemical or physico-chemical variables, e.g. pH-value characterised by the use of electric means

Abstract

A method for preparing an aqueous solution of a defined pH comprising an acid, a base and optionally one or more additives is provided. The method comprises the steps of: a) calculating the theoretical concentrations of acid and base for the solution to have the defined pH using the Henderson-Hasselbach equation in combination with the Debye Huckel theory for a range of different additive concentrations; b) preparing a sample of the buffer for the range of additive concentrations and measuring the actual pH for each additive concentration; c) calculating a value for delta pH, ∆pH, being the difference between the theoretical pH and the actual pH, for each additive concentration; d) generating a mathematical model describing the relationship of ∆pH with additive concentration; e) selecting the defined pH and additive concentrations;f) using the mathematical model generated in step d) to calculate ∆pH for the defined pH and additive concentration; g) calculating a ∆pH-corrected pH by summing the defined pH and delta pH; h) using the ∆pH-corrected pH to calculate the concentrations of acid and base using the Henderson-Hasselbach equation in combination with the Debye Huckel theory; i) preparing the solution using the concentrations calculated in step h).

IPC Classes  ?

• G01N 30/34 - Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
• G05D 21/02 - Control of chemical or physico-chemical variables, e.g. pH-value characterised by the use of electric means
• B01J 47/15 - Controlling or regulating for obtaining a solution having a fixed pH
• B01D 15/16 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier

Abstract

A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.

IPC Classes  ?

• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means (3) for mixing at least two liquids comprising a multiple inlet flow-controller (2), the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device (1) configured in single-pass mode.

IPC Classes  ?

• B01D 61/18 - Apparatus therefor
• B01D 61/20 - AccessoriesAuxiliary operations
• C07K 1/14 - ExtractionSeparationPurification
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01D 61/14 - UltrafiltrationMicrofiltration
• B01D 61/16 - Feed pretreatment
• B01D 61/22 - Controlling or regulating

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others. Scientific, industrial and technical research, development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; process development, namely, development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology.

Abstract

A protein expression system for use in a prokaryotic host is provided, the expression system comprising: a) an expression cassette comprising a nucleic acid sequence encoding a protein of interest operably linked to a T7 RNA polymerase-dependent promoter; and b) an expression cassette comprising a nucleic acid sequence encoding T7 RNA polymerase operably linked to a host polymerase-dependent λ phage promoter and a single perfect palindrome operator sequence; wherein the expression cassette for T7 RNA polymerase is located on the chromosome of a host cell.

IPC Classes  ?

• C12N 15/67 - General methods for enhancing the expression
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/73 - Expression systems using phage lambda regulatory sequences

Abstract

A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Abstract

A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12N 15/73 - Expression systems using phage lambda regulatory sequences
• C12R 1/19 - Escherichia coli
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow- controller, such as a valve.

IPC Classes  ?

• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means (3) for mixing at least two liquids comprising a multiple inlet flow-controller (2), the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device (1) configured in single-pass mode.

IPC Classes  ?

• B01D 61/18 - Apparatus therefor
• B01D 61/20 - AccessoriesAuxiliary operations
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow- controller, such as a valve.

IPC Classes  ?

• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means (3) for mixing at least two liquids comprising a multiple inlet flow-controller (2), the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device (1) configured in single-pass mode.

IPC Classes  ?

• B01D 61/18 - Apparatus therefor
• B01D 61/20 - AccessoriesAuxiliary operations
• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis

Abstract

A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Abstract

An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.

IPC Classes  ?

• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/62 - DNA sequences coding for fusion proteins

Goods & Services

Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries. Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others. Scientific, industrial and technical research, development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; process development, namely, development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology.

Abstract

A process for the production of a target polypeptide is provided. The process comprises expressing an expression cassette for the target recombinant polypeptide comprised in a vector in a non-mammalian host, wherein the host is auxotrophic for methionine and lacks the ability to express functional chromosomal Vitamin B12 independent homocysteine methyltransferase. The vector comprises an expression cassette for functional Vitamin B12 independent homocysteine methyltransferase and the host is cultured in a growth medium lacking methionine.

IPC Classes  ?

• C12N 15/65 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression using markers

Abstract

A DNA construct comprising a hamster beta-actin promoter operably linked to a cytomegalovirus enhancer is provided. Preferably the promoter is Chinese hamster beta- actin promoter, and the enhancer is the hCMV Major Intermediate Early enhancer. The DNA constructs can be employed for the production of polypeptides of interest, especially antibodies and antibody fragments.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Abstract

A process for production of a target recombinant polypeptide in a prokaryotic cell is provided. The process comprises expressing a vector comprising a first expression cassette comprising a polynucleotide encoding the target polypeptide operably linked to an inducible promoter and comprising a ribosome binding site; and a second expression cassette comprising a polynucleotide encoding an sRNA which targets the ribosome binding region of mRNA which corresponds to the ribosome binding site of the first expression cassette, operably linked to a promoter and wherein the sRNA comprises a sequence of bases complementary to at least a part of the ribosome binding region of the mRNA, which sequence comprises at least 8 consecutive bases in which no more than 15% of the bases exhibit non-Watson-Crick nucleotide base pairing to the ribosome binding region of the mRNA.

IPC Classes  ?

• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression

Abstract

A process for the production of a target recombinant polypeptide is provided. The process comprises expressing an expression cassette encoding the target polypeptide and co-expressing an expression cassette encoding a mitochondrial foldase. Preferred mitochondrial foldases include Mia 40 and Erv1, and homologs thereof.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/90 - Isomerases (5.)
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Abstract

A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/30 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Goods & Services

Processing and manufacturing of vectors, cell cultures and recombinant proteins for use in biotechnology industries to the order and specification of others; manufacture, processing and production of recombinant proteins for use in the biotechnology industries to the order and specification of others [ Cell cultures used in industry and science; mammalian cell cultures and vectors, being independent replicating pieces of DNA that can transfer DNA sequences, each for manufacture and development in the biotechnology industries ] Scientific, industrial and technical research, development and analysis services relating to the use of mammalian cell cultures and vectors in the field of biotechnology; process development, namely, development of processes for the use of mammalian cell cultures and vectors in the manufacture of biotechnology products; project studies relating to the use of mammalian cell cultures and vectors in the field of biotechnology

Abstract

S. cerevisiae GAL1 or GAL10 promoter. Vectors and methods for producing recombinant polypeptides are also provided.

IPC Classes  ?

• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12P 21/00 - Preparation of peptides or proteins

Abstract

A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12N 15/78 - Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 15/73 - Expression systems using phage lambda regulatory sequences
• C12R 1/19 - Escherichia coli

Abstract

In one aspect, a process for the intracellular expression of CRM197 in an E coli host is provided. The process comprises expressing a vector comprising a gene encoding CRM 197 operably linked to a promoter and at least one perfect palindrome operator sequence. In a related aspect, a process for the periplasmic expression of CRM197 in an E coli host is provided, comprising expressing a vector comprising a gene encoding CRM197 and a secretion leader sequence operably linked to a weak promoter system comprising at least one perfect palindrome operator sequence.

IPC Classes  ?

• A61K 47/48 - Medicinal preparations characterised by the non-active ingredients used, e.g. carriers, inert additives the non-active ingredient being chemically bound to the active ingredient, e.g. polymer drug conjugates
• C07K 14/34 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from bacteria from Corynebacterium (G)
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12N 15/73 - Expression systems using phage lambda regulatory sequences

Abstract

A process for the production of a target recombinant polypeptide is provided. The process comprises expressing a vector comprising a promoter operatively linked to an expression cassette for the target recombinant polypeptide; and a perfect palindrome operator sequence in a culture medium, wherein the culture medium is substantially free from antibiotic.

IPC Classes  ?

• C12N 15/67 - General methods for enhancing the expression
• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

A process for the purification of a high-potency polypeptide is provided. The process comprises contacting a crude mixture comprising a high-potency polypeptide and one or more impurities with a purification medium using a supply line; and contacting one or more processing solutions with the purification medium using a supply line, whereby the concentration of one or more impurities relative to the high-potency polypeptide is reduced, characterised in that the supply line for the high potency polypeptide is a disposable supply line separate from the supply line for the processing solutions.

IPC Classes  ?

• C07K 1/14 - ExtractionSeparationPurification

Abstract

A recombinant yeast of the genus Komagataella which has been transformed with a galactose promoter is provided. The yeast is preferably Komagataella phaffii, and the promoter is preferably a S. cerevisiae GAL1 or GAL10 promoter. Vectors and methods for producing recombinant polypeptides are also provided.

IPC Classes  ?

• C12R 1/78 - Hansenula
• C12R 1/84 - Pichia
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Abstract

A process for cell lysis is provided. The process comprises passing a mixture comprising a suspension of cells and a lysis reagent through a flow-through reactor, wherein the mixture passes through the reactor with pulsed or superimposed oscillating flow. The process is preferably employed for the preparation of biological products, such as pDNA or inclusion bodies.

IPC Classes  ?

• C12N 1/06 - Lysis of microorganisms
• C12N 1/00 - Microorganisms, e.g. protozoaCompositions thereofProcesses of propagating, maintaining or preserving microorganisms or compositions thereofProcesses of preparing or isolating a composition containing a microorganismCulture media therefor

Abstract

A process for the production of a target recombinant polypeptide is provided. The process comprises expressing a vector comprising a promoter operatively linked to an expression cassette for the target recombinant polypeptide; and a perfect palindrome operator sequence in a culture medium, wherein the culture medium is substantially free from antibiotic.

IPC Classes  ?

• C12R 1/19 - Escherichia coli
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon
• C12N 15/67 - General methods for enhancing the expression

Abstract

A process for the extraction of pDNA from cells is provided. In one aspect, the process comprises heating a liquid comprising the cells to an average temperature of from 95° C. to about 120° C. for a time of less than 10 seconds. In certain preferred aspects, the pDNA is extracted by the use of flow-through apparatus.

IPC Classes  ?

• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

A process for the production of a target recombinant polypeptide is provided. The process comprises expressing an expression cassette encoding the target polypeptide and co- expressing an expression cassette encoding a mitochondrial foldase. Preferred mitochondrial foldases include Mia 40 and Erv1, and homologs thereof.

IPC Classes  ?

• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase

Abstract

A process for purification of a fragment antibody from a culture medium also comprising at least one impurity is provided. The purification is carried out after the pH of the culture medium has been reduced to a pH at which the fragment antibody is soluble, but one or more of the impurities are insoluble. A process for the preparation of a fragment antibody employing such a purification process is also provided.

IPC Classes  ?

• C07K 1/14 - ExtractionSeparationPurification
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• C07K 1/30 - ExtractionSeparationPurification by precipitation
• C07K 16/06 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies from serum
• C07K 16/24 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
• C07K 19/00 - Hybrid peptides
• C07K 16/00 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies
• C07K 16/40 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against enzymes

Abstract

An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.

IPC Classes  ?

• C12N 15/62 - DNA sequences coding for fusion proteins
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli

Abstract

A process for preparing a conditioned cell culture medium is provided. The process comprises a) culturing eukaryotic cells in a growth medium having a composition effective to support cell growth; b) separating the cultured cells from the growth medium; and c) maintaining the cultured cells in a basal medium having a composition suitable to maintain cell viability, but not to support substantial cell growth. The cells are preferably dermal sheath, dermal papilla or dermal fibroblast cells. The compositions are useful as pharmaceutical compositions, especially for wound healing.

IPC Classes  ?

• C12N 5/071 - Vertebrate cells or tissues, e.g. human cells or tissues
• A61K 35/36 - SkinHairNailsSebaceous glandsCerumenEpidermisEpithelial cellsKeratinocytesLangerhans cellsEctodermal cells
• A61P 17/02 - Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like

Abstract

A process for the extraction of pDNA from cells is provided. In one aspect, the process comprises heating a liquid comprising the cells to an average temperature of from 95°C to about 120°C for a time of less than 10 seconds. In certain preferred aspects, the pDNA is extracted by the use of flow-through apparatus.

IPC Classes  ?

• C12N 1/06 - Lysis of microorganisms
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

An expression system for the production of a target polypeptide is provided. The expression system comprises an expression cassette comprising an inducible promoter operably linked to DNA encoding the target polypeptide and an expression cassette for over-expression of DNA binding transcriptional regulator protein, comprising a promoter operably linked to DNA encoding the DNA binding transcriptional regulator protein. The expression cassettes are under the control of orthogonal promoters.

IPC Classes  ?

• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/72 - Expression systems using regulatory sequences derived from the lac-operon

Abstract

b) an expression cassette for T7 RNA polymerase operably linked to a λpL promoter and at least two perfect palindrome operator sequences.

IPC Classes  ?

• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material

Abstract

A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor

Goods & Services

(Based on 44(e)) [ Microbially produced chemicals used in industry and science; microbial preparations used in industry and science; proteins, enzymes, and cultures of micro organisms used in industry and science; chemical reagents for non-medical purposes; genes being DNA sequences for manufacture and development in the biotechnology and pharmaceutical industries; plasmids being small independently replicating pieces of DNA and vectors being small independent replicating pieces of DNA that can transfer DNA sequences all for manufacture and development in the biotechnology and pharmaceutical industries ] (Based on Use in Commerce) Processing and manufacturing of chemicals and proteins for use in biotechnology and pharmaceutical industries to the order and specification of others; manufacture, processing and production of scientific and industrial products, namely, chemicals and proteins for use in the biotechnology and pharmaceutical industries to the order and specification of others (Based on Use in Commerce) Scientific, industrial and technical research, development and analysis services in the field of biotechnology and pharmaceuticals; process development, namely, development of chemicals for use in the manufacture of biotechnology products and pharmaceuticals; industrial design services; provision of facilities for scientific, industrial and technical research development in the field of biotechnology and pharmaceuticals; research and development of scientific, industrial and technical products; projects studies in the field of biotechnology and pharmaceuticals; testing of scientific, industrial and technical products in the field of biotechnology and pharmaceuticals

Goods & Services

Chemicals used in industry and science; proteins, enzymes; microbial preparations; cultures of micro-organisms; chemical reagents; genes being DNA sequences; plasmids being small independently replicating pieces of DNA and vectors being small independently replicating pieces of DNA that can transfer DNA sequences. Processing and manufacturing of chemicals and proteins, including to the order and specification of others; manufacture, processing and production of scientific and industrial products to the order and specification of others. Scientific, industrial and technical research, development and analysis services; process development; industrial design services; provision of facilities for scientific, industrial and technical research development; research and development of scientific, industrial and technical products; project studies; testing of scientific, industrial and technical products.

Abstract

A protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence. In an embodiment, the promoter is T7A1, T7A2, T7A3, .lambda.pL, .lambda.pR, lacUV5, trp, trc, phoA and rrnB. In another embodiment, the promoter is an E. coli polymerase-dependent promoter, and the perfect palindrome operator sequence overlaps with the transcriptional start point. In another embodiment, there is proved a vector comprising a yeast promoter, and a perfect palindrome operator sequence. In another embodiment, there is provided a method for the production of a protein which comprises expressing, in a mammalian cell culture, an expression system comprising: a) a mammalian promoter; and b) two or more perfect palindrome operator sequences, at least one operator sequence being located downstream of the promoter, and at least one operator sequence being located upstream of the promoter; and c) an expression cassette for a recombinant protein.

IPC Classes  ?

• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 1/19 - YeastsCulture media therefor modified by introduction of foreign genetic material
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/81 - Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.

IPC Classes  ?

• C12N 15/70 - Vectors or expression systems specially adapted for E. coli
• C12N 15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
• C12N 1/21 - BacteriaCulture media therefor modified by introduction of foreign genetic material
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione