Abstract

Provided are a multiple liquid chip detection method and a kit, using an HSV‑1 viral culture and BV gD, SIV p27, SRV p27 and HTLV‑1envelope recombinant proteins as antigens of BV, SIV, SRV and STLV respectively to coat magnetic beads with different numbers, using the magnetic beads coated with different antigens as carriers, to establish a multiple liquid chip detection method capable of simultaneously detecting four antibodies, namely, monkey BV and other three retroviruses.

IPC Classes  ?

• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 21/64 - FluorescencePhosphorescence
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

Disclosed are a multi-liquid phase gene chip detection primer, a kit and a method for rapidly distinguishing five pathogens in mouse respiratory tracts. The present invention obtains a target amplified fragment by PCR, and then hybridizes the amplified product, a fluorescence-encoded microsphere and streptavidin-phycoerythrin, and reads the MFI value via a detector to distinguish different types of viruses.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

Disclosed are a multiplex fluoroimmunoassay method for rapidly distinguishing the rabbit hemorrhagic disease virus, Sendai virus and lapine rotavirus, and a reagent. The fluoroimmunoassay method comprises: obtaining a target amplification fragment by means of PCR; then hybridizing an amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin; reading the MFI value through a detector; and distinguishing different types of viruses.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12R 1/93 - Animal viruses

Abstract

Disclosed is a PCR-HRM detection method for rapidly identifying a PRRSV vaccine strain, i.e. GDr180 strain, and other virulent strains. The method of the present invention is based on PCR in combination with HRM, comprising a first round of PCR conventional amplification and a second round of PCR-HRM, and the addition of a fluorescence-saturated dye before the reaction.

IPC Classes  ?

• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12R 1/93 - Animal viruses

Abstract

The present invention discloses an RT-PCR-HRM or PCR-HRM primer, reagent, and method for rapidly distinguishing four types of serum dengue virus.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12R 1/93 - Animal viruses