The present invention relates to the technical field of veterinary biological products, and in particular to a positive serum for a lumpy skin disease virus, and a preparation method therefor and a use thereof. Healthy negative cattle are inoculated with an inactivated vaccine, the number of inoculations is greater than or equal to 2, and the dose of the inactivated vaccine inoculated each time is larger than or equal to 6 mL, so that a positive serum for a lumpy skin disease virus is finally prepared. The positive serum for a lumpy skin disease virus has a neutralizing antibody titer of ≥1:32, and can be fully applicable to each test item involved in the vaccine development process, so that the present invention solves the bottleneck problem of quality control in the development process of inactivated vaccines for lumpy skin diseases, fills the gap in the preparation process of positive serums for a lumpy skin disease virus, and has important significance for long-term development of veterinary biological product industry.
The present invention relates to the technical field of veterinary vaccines, and in particular to an immunopotentiator, a preparation method therefor, and a use thereof. The immunopotentiator of the present invention comprises liposomes and CpG nucleotides in a specific ratio. Furthermore, the immunopotentiator of the present invention preferably further comprises astragalus polysaccharide and angelica polysaccharide in a specific ratio.
A heat-resistant protective agent for a swine erysipelas live vaccine, a preparation method therefor, and a use thereof. The heat-resistant protective agent comprises the following components in mass/volume percent: 8% to 12% of maltodextrin, 3% to 5% of gelatin, 1% to 2% of L-arginine hydrochloride, 1% to 3% of polyvinylpyrrolidone, 1% to 3% of L-histidine hydrochloride, 6% to 8% of enzyme-hydrolyzed casein, 3% to 5% of D-sorbitol, 1% to 2% of sodium thiosulfate, 3% to 6% of tert-butyl alcohol, and 1% to 3% of polyethyleneimine, the remainder being water for injection. According to the heat-resistant protective agent, a swine erysipelas live vaccine can be stably stored for a long time, the decrease in the titer of live bacteria in the swine erysipelas live vaccine is inhibited, immune efficacy is ensured, and the swine erysipelas live vaccine also has a good freeze-dried appearance, a relatively low water content and good dissolution characteristics.
Provided are a Feline calicivirus cultivation method and a virus fluid. The Feline calicivirus cultivation method comprises: cultivating host cells by using a microcarrier, performing virus inoculation, and continuing cultivation to obtain a virus fluid, wherein the cell density of a host cell suspension is 1.8x106cells/mL to 2.2x106cells/mL; the addition amount of the microcarrier in the host cell suspension is 2.5-3.5 g/L; and the microcarrier uses cross-linked glucan as a matrix, and has a density of 1.01-1.05 g/mL, a particle size of 185-195 μm, and a surface area of greater than or equal to 4200 cm2. The titer of the Feline calicivirus fluid prepared by the method of the present invention is greater than or equal to 1010.55050.
Provided are an Akabane disease virus strain and a use thereof. The Akabane disease virus strain is Akabane disease virus AKAV/JL/2022, and the accession number thereof is CGMCC No. 45375. The Akabane disease virus strain has excellent passage stability, high pathogenicity and excellent immunogenicity, can induce a body to generate high-titer neutralizing antibodies, can target epidemic Akabane disease after being prepared into a vaccine, and can be used in the control and treatment of Akabane disease in a pasturing region.
A heat-resistant protective agent for a live vaccine, a preparation method therefor, and use thereof. The protective agent for a live vaccine comprises: an inulin, gelatin, glycine, polyvinylpyrrolidone, bovine serum albumin, proteolyzed casein, D-sorbitol, a water-soluble phospholipid, pollen pini, and a tocopherol, wherein the mass ratio of pollen pini to the tocopherol is (1-2):1. The components of the protective agent are simple and effective, the raw materials are safe and readily available, and the preparation method is convenient and fast. When the protective agent is used to prepare a live Mycoplasma bovis vaccine, the protective agent can effectively reduce the loss rate of live mycoplasma bacteria during lyophilization and extend the shelf life, and meanwhile the vaccine can induce a long-lasting, efficient immune response in an organism.
Provided is a virus maintenance liquid for ruminant poxvirus culture and use thereof in full-suspension culture of a ruminant poxvirus. The provided virus maintenance liquid for ruminant poxvirus culture is prepared from a dry powder composition of a plurality of virus maintenance liquids, and comprises 5%-20% of a dry powder of maintenance liquid-1 and 5%-20% of a dry powder of maintenance liquid-2, and further comprises 50%-90% of one of a dry powder of maintenance liquid-3 and a dry powder of maintenance liquid-4 or a mixture of the two dry powders in any ratio. When the virus maintenance liquid is used for full-suspension culture of a ruminant poxvirus, the virus titer in a harvested virus liquid can be significantly improved, and the cost of vaccine production can be further reduced.
A method for inactivating viruses and the use are provided. The method uses formaldehyde as an inactivation agent; and the method further comprises a formaldehyde blocking step after virus inactivating with formaldehyde, active ingredients of a blocking agent used in the formaldehyde blocking step being sodium bisulfite and EDTA-2Na, and the mass ratio of the sodium bisulfite to the EDTA-2Na being (3.8-4.2):1. The method can effectively reduce the toxicity problem of formaldehyde when formaldehyde is used as an inactivation agent, reduce the residual amount of formaldehyde, and is particularly suitable for lumpy skin disease virus inactivation and formaldehyde blocking.
The present invention relates to the technical field of veterinary biological products. Disclosed are a composition for removing endotoxin from bovine serum and use. The composition for removing endotoxin from bovine serum comprises a removal agent and an adsorbent, wherein the removal agent comprises Triton X-114, and the adsorbent comprises activated carbon. The removal agent can effectively remove endotoxin from bovine serum, and the adsorbent can effectively remove the adverse effect of the removal agent on bovine serum.
The present invention relates to the technical field of veterinary biological products, and specifically, to a lumpy skin disease virus strain, an inactivated vaccine prepared from the strain, and a method for preparing the vaccine. The lumpy skin disease virus strain LSDV/CH/JY/2021 features high virulence, good immunogenicity, high homology with existing strains, and suitability for producing broad-spectrum inactivated vaccines. The inactivated LSDV vaccine features high safety, high immune potency, and suitability for industrial massive production, and is favorable for the prevention and control of lumpy skin disease.
The present application relates to an attenuated African swine fever virus strain. Compared with a wild type genotype II African swine fever virus, the present attenuated African swine fever virus strain has the following gene fragments deleted in the genome: the CD2V and I177L gene fragments.
HEAT-RESISTANT PROTECTIVE AGENT FOR PORCINE EPIDEMIC DIARRHEA AND SWINE TRANSMISSIBLE GASTROENTERITIS COMBINED LIVE VACCINE, PREPARATION METHOD THEREFOR, AND USE THEREOF
Disclosed are a heat-resistant protective agent for porcine epidemic diarrhea and swine transmissible gastroenteritis combined live vaccine, a preparation method therefor, and use thereof. The heat-resistant protective agent comprises 5-10 parts by weight of sucrose, 5-10 parts by weight of maltodextrin, 0.5-1 part by weight of ascorbic acid, 1-3 parts by weight of polyvinylpyrrolidone, 1-3 parts by weight of glycine, 1-2 parts by weight of carboxymethyl cellulose, and 3-5 parts by weight of tryptone. When the heat-resistant protective agent is used for preparing the porcine epidemic diarrhea and swine transmissible gastroenteritis combined live vaccine, the loss of each viral antigen in the freeze-drying process can be effectively reduced, and the immune efficacy and long-term stability of the porcine epidemic diarrhea and swine transmissible gastroenteritis combined live vaccine formulation can be kept.
Provided are a water-in-oil adjuvant for a poultry animal vaccine, a preparation method therefor and use thereof, which belong to the technical field of animal vaccines in the category of biological products. Raw materials of the water-in-oil adjuvant provided herein comprise, by weight in percentage: 85 wt%-90 wt% of oil for injection, 5 wt%-10 wt% of refined Span 80 and 1 wt%-5 wt% of refined Tween 80, and can further comprise 0.1 wt%-1 wt % of an immunostimulatory complex. A vaccine prepared by using the water-in-oil adjuvant provided herein is stable in quality and high in safety, and can induce a body to generate a longer-duration and more efficient immunoreaction, so that the water-in-oil adjuvant can be used as a safe and effective adjuvant for a poultry animal vaccine and the like.
Disclosed are a bovine Pasteurella multocida capsular type A capsular polysaccharide vaccine and a preparation method therefor, and belongs to the technical field of veterinary vaccines. The capsular polysaccharide vaccine provided is prepared from bovine Pasteurella multocida capsular type A capsular polysaccharide and a vaccine adjuvant made from raw materials including refined Span-80 and refined Tween-80. The capsular polysaccharide vaccine can effectively prevent and control bovine cellulose suppurative pneumonia caused by A-type Pm, has good safety, and can be used for providing a safe and effective protection effect for healthy and susceptible calves, healthy pregnant cows and healthy milk cows.
A whole genome sequence of the lumpy skin disease virus LSDV/CH/JY/2021 and an amplification primer thereof. The primer provided for PCR amplification of the whole genome sequence of the lumpy skin disease virus consists of 30 pairs of primers, and 30 nucleotide sequence fragments obtained by amplifying these primer pairs are sequentially spliced, edited and corrected, and are used to complete a nucleotide sequence fragment of the lumpy skin disease virus obtained using the next-generation sequencing method to obtain the whole genome sequence of the lumpy skin disease virus. The provided whole genome sequence of the lumpy skin disease LSDV/CH/JY/2021 is beneficial to the research into the pathogenesis of the virus and the like, and is beneficial to the development of diagnostic reagents and vaccines for the virus and the like.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
16.
GENE-DELETED ATTENUATED AFRICAN SWINE FEVER VIRUS STRAIN, AND CONSTRUCTION METHOD THEREFOR AND USE THEREOF
SHANGHAI VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES (China)
Inventor
Song, Qingqing
Ye, Zhengqin
Chen, Hongjun
Wang, Heng
Di, Dongdong
Wu, Jinxian
Gong, Lang
Tu, Jie
Xie, Zhenhua
Chen, Jian
Liu, Jianqi
Xu, Liyuan
Zhao, Lixia
Liu, Yingnan
Zhang, Chongyu
Abstract
The present invention belongs to the technical field of biological vaccine products. Disclosed are a gene-deleted attenuated African swine fever virus strain, and a construction method therefor and the use thereof. In the present invention, the gene-deleted attenuated African swine fever virus strain constructed by means of a homologous recombination method is a gene-deleted virus strain in which CD2v, MGF (12L, 13L, 14L) and I177L gene segments are simultaneously deleted on the basis of the type II African swine fever virus genome, and is obviously attenuated relative to a parental strain, and the stable replication and immunogenicity of the gene-deleted virus strain is not affected. After vaccinating laboratory pigs with the gene-deleted attenuated African swine fever virus strain, the phenomena of a significant rise in body temperature, joint swelling, pathogenesis or death in the laboratory pigs do not appear, and viremia does not occur, and thus, the gene-deleted attenuated African swine fever virus strain shows good safety and a good protection effect against immunity challenge. Therefore, the gene-deleted attenuated African swine fever virus strain provided by the present invention can be used as a candidate vaccine strain with good safety and a good immune protection effect.
Provided is a dual real-time fluorescent quantitative PCR detection reagent kit for identifying Seneca virus A and a foot-and-mouth disease virus. The reagent kit comprises two pairs of primers and two probes; the primer and the probe are respectively designed for the 3C region genes of Seneca virus A and the foot-and-mouth disease virus.
C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage
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