Abstract

Shewanella decolorationis S3-4, is deposited in the China General Microbiological Culture Collection Center (CGMCC) on Mar. 28, 2022, with a deposit number of CGMCC No. 24602. The strain can secrete a same substance as tetrodotoxin from Tetraodontidae.

IPC Classes  ?

• C12N 1/20 - BacteriaCulture media therefor
• A61K 39/00 - Medicinal preparations containing antigens or antibodies

Abstract

The present invention provides a genomic selection method for Plectropomus leopardus disease-resistant improved variety breeding. According to the method, a disease-resistant reference population is constructed by using Plectropomus leopardus juveniles of non-family populations from different geographical origins, the Plectropomus leopardus juveniles used to construct the disease-resistant reference population being obtained after disease resistance screening; individuals which have disease resistance phenotypes but are not incorporated into the reference population are used as a verification population, to verify the effect of calculating genomic estimated breeding values using SNP loci provided in the present invention, the SNP loci comprising SNP loci related to Plectropomus leopardus disease resistance; genomic estimated breeding values of a candidate population are calculated by using the SNP loci provided in the present invention, the individual having a high genomic estimated breeding value having strong disease resistance, and said individual being capable of being used for Plectropomus leopardus disease-resistant improved variety breeding. The Plectropomus leopardus disease-resistant improved variety breeding method based on the genomic selection technology provided in the present invention can be used for screening for a parent having strong disease resistance, the selected parent can be used for Plectropomus leopardus disease-resistant selective breeding, and a technical means is provided for improving the survival rate of the Plectropomus leopardus breeding population.

IPC Classes  ?

• C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

Abstract

Stichopus japonicus, the use of the pesticides and fishery drugs in the culture and pond-cleaning process is reduced, and green and healthy culture, energy saving and environmental protection are realized.

IPC Classes  ?

• A01K 61/00 - Culture of aquatic animals
• A01G 33/00 - Cultivation of seaweed
• A01K 61/30 - Culture of aquatic animals of sponges, sea urchins or sea cucumbers
• A01K 61/59 - Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps

Abstract

Disclosed is a method for green and pollution-free breeding of Apostichopus japonicus, Marsupenaeus japonicus, Portunus trituberculatus, and Ulva lactuca, belonging to the technical field of aquaculture, said method comprising the following steps: in early March, cleaning up a pond and laying an Apostichopus japonicus attachment base; in early April, dispensing Apostichopus japonicus seeds, and subsequently cultivating Ulva lactuca seedlings; in mid-April, dispensing Marsupenaeus japonicus seeds; in early May, dispensing juvenile Portunus trituberculatus crabs; in mid-July, harvesting the Marsupenaeus japonicus and dispensing Marsupenaeus japonicus seeds again; in early November, harvesting Apostichopus japonicus, Marsupenaeus japonicus, Portunus trituberculatus, and Ulva lactuca; The present invention involves cultivation of sea cucumber, prawn, crab, and algae together, making effective use of the space of a body of water, reducing cultivation production costs, and increasing cultivated output; a method for biological control of organisms hostile to Apostichopus japonicus is used, reducing the use of pesticides and fishery drugs during the cultivation process and during the pond cleaning process, achieving green and healthy cultivation and energy conservation and environmental protection.

IPC Classes  ?

• A01K 61/59 - Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
• A01G 33/00 - Cultivation of seaweed

Abstract

An ecological conservation system and method for an artificial coastal wetland are provided. The system can include a fish and shrimp ecological breeding area, an artemia breeding area and a bromine extraction and salt production area, where a water inlet channel is arranged on a side of the fish and shrimp ecological breeding area; a sedimentation and biological purification channel is arranged between the fish and shrimp ecological breeding area and the artemia breeding area; a drainage channel is arranged between the artemia breeding area and the bromine extraction and salt production area; and waterfowl habitat islands are built in the fish and shrimp ecological breeding area and the artemia breeding area.

IPC Classes  ?

• A01K 61/59 - Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
• A01K 61/10 - Culture of aquatic animals of fish

Abstract

M. japonicus. Therefore, it has a broad application prospect.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

Abstract

An antibiotic susceptibility testing device of gram-negative bacteria, as well as a corresponding method, are discussed. The device has a temperature control unit (including a constant temperature chamber) and a contactless conductivity-based measurement system. Disposable glassy or PVC tubes are used as test vessels for AST. In the performance of AST assay, appropriate kind of liquid medium containing identical amount of target bacterial cells and target antibiotics at different concentrations are loaded into test tubes, following by incubation in the device at a setup temperature. The bacterial growth profile is monitored by collecting the differential values (ΔC) of conductivity of liquid medium, which depend on the proliferation of viable cells. Outcome of ΔC indicates whether the target bacterial cells are completely inhibited by the test antibiotic or not, enabling the user to judge the value of the minimal inhibitory concentration (MIC) simply.

IPC Classes  ?

• C12Q 1/18 - Testing for antimicrobial activity of a material
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/00 - Apparatus for enzymology or microbiology
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• G01N 15/06 - Investigating concentration of particle suspensions

Abstract

Provided is a gene chip for the selective breeding of disease-resistant fine breeds of olive flounder, which solves the problem of lack of a gene chip in breeding of fine fish species, overcomes the defects of the traditional breeding technology, provides a new molecular breeding method for the breeding of disease-resistant, high-yield, and high-quality fine fish species, provides a technical means for the selective breeding of disease-resistant fine breeds for fish farming industry, updates the fish breeding technology, and promotes the rapid development of the fish seed industry. The provided gene chip based on an SNP site related to disease resistance of olive flounder can be used for the selective breeding of disease-resistant individuals of olive flounder, and the actual selection accuracy is proximate to the theoretical value. Therefore, the accuracy of the selection of the disease-resistant fine breeds of olive flounder can be improved, the breeding cycle can be shortened, the gene chip technology is provided for the selective breeding of the disease-resistant fine breeds of olive flounder, and a new way of gene chip breeding is opened up for the selective breeding of disease-resistant fine fish species.

IPC Classes  ?

• C12Q 1/6888 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
• C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

A method of using EA-IRMS to analyze particulate organic carbon and nitrogen stable isotopes in seawater. The method comprises: filtering an acquired water sample using a glass fiber filter, and then filtering 100 ml of distilled water; and acidifying a filter sample, and placing same in a machine for EA-IRMS analysis, wherein the oxygen content of an elemental analyzer is configured to 80s, the temperature of a combustion tube is configured to 700℃, and the filament current of an isotope ratio mass spectrometer is configured to 300-400 μA. The method can reduce testing costs, improve data accuracy, and improve operational efficiency.

IPC Classes  ?

• G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosolsInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
• G01N 31/12 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using combustion
• G01N 1/34 - PurifyingCleaning

Abstract

The present invention provides an automatic drug sensitivity test apparatus of gram-negative bacteria, comprising: a temperature control apparatus having a constant temperature cavity in which a plurality of test working channels is arranged, each test working channel comprising a receiving electrode L, a receiving electrode S, and an exciting electrode A which are coaxially arranged successively, and the three electrodes being perpendicularly fixed in the constant temperature cavity by means of a fixed plate; a disposable detection tube provided in the test working channel; and a disposable bacteria filter detachably connected to the top end of the disposable detection tube. The present invention also provides an application method for the automatic drug sensitivity test apparatus of gram-negative bacteria.

IPC Classes  ?

• C12Q 1/04 - Determining presence or kind of microorganismUse of selective media for testing antibiotics or bacteriocidesCompositions containing a chemical indicator therefor
• C12M 1/38 - Temperature-responsive control
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means

Abstract

The present invention relates to the field of bioengineering, and relates specifically to a contactless gene amplification electrochemical rapid detection method, applicable in gene amplification process monitoring and result analysis. A change in the ionic strength of an amplification reaction solution is measured by coupling a contactless electrical conductivity sensor via a capacitor, combined with a reaction time factor, and qualitative and quantitative information of target gene fragment in a sample DNA or RNA is produced in a simple, rapid, and automated manner at reduced costs.

IPC Classes  ?

• G01N 27/06 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
• G01N 27/02 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance

Abstract

An expression vector for increasing protein expression efficiency is provided. A specific nucleotide sequence is introduced into the downstream of the initiation codon ATG of the expression vector, and encodes at least 3 amino acid residues. These amino acid residues process 4 or more codons. A corresponding method for increasing protein expression efficiency is also provided. The method of the present invention can be used in modification of any gene for increasing its expression efficiency. As compared to prior art, the expression vector or method of the present invention can increase the target protein expression efficiency by 2-5 times.

IPC Classes  ?

• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/67 - General methods for enhancing the expression

Abstract

Cynoglossus semilaevis gender-specific microsatellite markers and an application thereof in super-female fish identification, wherein the nucleotide sequences of the gender-specific microsatellite markers are SEQIDNO:1 and SEQIDNO:2 respectively. A pair of primers for expanding the gender-specific microsatellite markers, an upstream primer and a downstream primer thereof have sequences SEQIDNO:3 and SEQIDNO:4 respectively. A method using the primers to identify the gender of a cynoglossus semilaevis: the amplified product segment of a super-female being 218 bp; the amplified product of females being two DNA segments, 216 – 220 bp and 204 – 208 bp in size respectively; the amplified product for males being one DNA segment, of 204 – 208 bp in size. Employment of the microsatellite markers enables rapid identification of cynoglossus semilaevis ZE female, WW super-female, and ZZ male, thereby solving a difficulty found in gender control of cynoglossus semilaevis, and in WW super-female and ZW female identification in a same-sex culture seeding.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Abstract

An endogenous antiviral complex-probiotic-preparation for prawn. The preparation consists of 5-20% by weight of Bacillus firmus SSL065 powder, 5-10% by weight of Vibrio alginolyticus SSL073 powder and 70-90% by weight of carriers. The two bacteria components have synergistic effect. The present preparation has multiple biological functions such as obvious improvement of cultured prawn's nonspecific immunity level, great reinforcement of cultured prawn's resistance to pathogenic bacteria infection, and especially significant improvement cultured prawn's resistance to viral pathogen infection.

IPC Classes  ?

• A61K 39/07 - Bacillus
• A61K 39/106 - VibrioCampylobacter
• A61P 31/12 - Antivirals

Abstract

The invention provides gene chips for detecting multiple pathogenic bacteria in animals cultivated in sea water and uses thereof. The pathogenic bacteria include: Vibrio anguillarum, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio vulnificus, Non-O1 Vibrio cholerae, Vibrio fluvialis, Vibrio fischeri, Vibrio splendidus, Vibrio mimicus, Vibrio salmonicida, Aeromonas hydrophila, Aeromonas sobria, Aeromonas salmonicida, Edwardsiella tarda, Edwardsiella ictaluri, Renibacterium salmoninarum, Mycobacterium marinum, Pseudomonas, and Streptococcus.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C40B 40/06 - Libraries containing nucleotides or polynucleotides, or derivatives thereof
• C12R 1/63 - Vibrio
• C12R 1/32 - Mycobacterium
• C12R 1/38 - Pseudomonas
• C12R 1/46 - Streptococcus

Abstract

The invention provides a multi-component adjuvant of immersion vaccine for fish, comprising N-acetylcysteine, Triton X-100, saponin and raceanisodamine. The working concentrations of said components are 0.1-10 mg/l, 0.1-10 mg/l, 0.1-50 mg/l and 1-60 mg/l respectively. The application of said adjuvant in immersion immunization for fish and the using method thereof are also provided.

IPC Classes  ?

• A61K 39/39 - Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
• A61P 31/04 - Antibacterial agents
• A61P 37/04 - Immunostimulants