Abstract

The present invention is applicable to the field of biological technology, and provides a B cell linear epitope, an antibody, an identification method, and an application of a novel coronavirus S protein. The amino acid sequence of the B cell linear epitope of the S protein is as shown in SEQ ID NO. 104 or SEQ ID NO. 82. The present invention provides a novel B cell linear epitope of the novel coronavirus S protein, which can be used to detect specific antibodies in the blood of COVID-19 patients, and can also be used to inoculate healthy people to induce the production of specific antibodies, thereby providing important antigen targets for SARS-CoV-2 vaccine design, monoclonal antibody development, and antibody detection kit development.

IPC Classes  ?

• C07K 14/165 - Coronaviridae, e.g. avian infectious bronchitis virus
• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
• G01N 33/569 - ImmunoassayBiospecific binding assayMaterials therefor for microorganisms, e.g. protozoa, bacteria, viruses
• A61K 39/215 - Coronaviridae, e.g. avian infectious bronchitis virus
• A61P 31/14 - Antivirals for RNA viruses

Abstract

The present invention relates to a capsaicin hapten and artificial antigen for detecting illegal cooking oil, a preparation method therefor and an application thereof. The structure of the capsaicin hapten is shown as formula I or formula II; the capsaicin artificial antigen is obtained by coupling the hapten shown in formula I or formula II with carrier protein. By immunizing animals with the capsaicin artificial antigen, specific antibodies with high titer and high sensitivity can be obtained. The capsaicin hapten and the antibodies prepared using the same provided by the present invention provide a new means for establishing a rapid, simple, cheap, sensitive and specific capsaicin detection method.

IPC Classes  ?

• C07C 231/12 - Preparation of carboxylic acid amides by reactions not involving the formation of carboxamide groups
• C07C 233/18 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
• C07C 233/20 - Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a carbon atom of an acyclic unsaturated carbon skeleton
• C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor

Abstract

Disclosed are a toxoflavin hapten, artificial antigen and antibody, synthesis methods therefor and applications thereof. An aliphatic chain connecting arm is derived on a nitrogen atom at position 1 of toxoflavin, and the terminal of the derived aliphatic chain connecting arm is provided with carboxyl. The toxoflavin hapten maximally retains a feature structure of the toxoflavin, and an antigenic determinant of the hapten can be exposed better, so that the immunogenicity of the toxoflavin hapten is obviously enhanced, and the toxoflavin hapten has the carboxyl which can be coupled with a carrier protein. The toxoflavin hapten is coupled with the carrier protein to obtain a toxoflavin immunizing antigen for immunizing an animal, which is more beneficial for stimulating immune response of the animal to generate an antibody having stronger specificity and higher sensitivity. An immunological method using the antibody can detect 0.02 ng/ml toxoflavin contamination, and provides a simple, convenient and rapid solution for bongkrek acid intoxication. The whole detection process only requires over ten minutes, and actual detection requirements can be completely met.

IPC Classes  ?

• C07D 487/04 - Ortho-condensed systems
• C07K 14/765 - Serum albumin, e.g. HSA
• C07K 14/77 - Ovalbumin
• C07K 14/79 - Transferrins, e.g. lactoferrins, ovotransferrins
• C07K 16/44 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere
• G01N 33/577 - ImmunoassayBiospecific binding assayMaterials therefor involving monoclonal antibodies

Abstract

Disclosed are methods for preparing a hapten of toxoflavin and an artificial antigen of toxoflavin, wherein a highly specific and sensitive antibody can be obtained from an animal immunized with the prepared artificial antigen. The antigen and antibody prepared and the treatment of a sample by means of this method can provide a simple and quick technical approach for the highly sensitive detection of toxoflavin.

IPC Classes  ?

• C07D 487/04 - Ortho-condensed systems
• C07K 14/765 - Serum albumin, e.g. HSA
• C07K 14/77 - Ovalbumin
• C07K 14/795 - Porphyrin- or corrin-ring-containing peptides
• C07K 16/44 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• G01N 33/532 - Production of labelled immunochemicals
• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody
• G01N 33/58 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving labelled substances

Abstract

Disclosed are a kit for detecting an MCR gene, a detection method and the use thereof. The kit comprises PCR group A primers and PCR group B primers, wherein the PCR group A primers comprise: a primer pair shown as SEQ ID NO: 1 and SEQ ID NO: 2, a primer pair shown as SEQ ID NO: 3 and SEQ ID NO: 4, a primer pair shown as SEQ ID NO: 5 and SEQ ID NO: 6, a primer pair shown as SEQ ID NO: 7 and SEQ ID NO: 8 and a primer pair shown as SEQ ID NO: 9 and SEQ ID NO: 10; the PCR group B primers comprise: a primer pair shown as SEQ ID NO: 11 and SEQ ID NO: 12, and a primer pair shown as SEQ ID NO: 13 and SEQ ID NO: 14.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids

Abstract

A method for detecting capsaicin in oil The method comprises the following steps: using immunoaffinity to separate and extract capsaicin from oil in advance; and using immunochromatography to perform detection on the separated and extracted capsaicin molecules. Using immunoaffinity to separate and extract the capsaicin molecules from oil comprises diluting an oil sample with a solution containing methanol and nano titanium dioxide and adding immunomagnetic beads which can specifically bind to capsaicin molecules to capture capsaicin molecules therein. In the method, combination of immunochromatography and immunoaffinity greatly reduces workload associated with detection of capsaicin in oil, and increases detection sensitivity.

IPC Classes  ?

• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

A detection device for quantitatively detecting the content of a target substance in a sample. By means of comparing the signal intensity in a detection area (11) and the signal intensity in a reference area (12), target molecules in a sample can be quantitatively detected. The detection device has low production costs, and accurate and reliable detection results.

IPC Classes  ?

• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody

Abstract

A disinfectant for killing bacteria, fungi and mycoplasmas in cells. Specifically involved are a liquid for removing microbial contamination based on povidone-iodine and a use method therefor, wherein the liquid contains povidone-iodine and PEG8000. The method has the advantages of being simple to operate and thorough sterilization.

IPC Classes  ?

• A01N 59/12 - Iodine, e.g. iodophorsCompounds thereof
• A01N 25/02 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
• A01N 25/22 - Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of applicationSubstances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
• A01N 59/06 - AluminiumCalciumMagnesiumCompounds thereof
• A01P 1/00 - DisinfectantsAntimicrobial compounds or mixtures thereof
• A01P 3/00 - Fungicides

Abstract

The present invention falls within the technical field of biological preparations, and particularly relates to a RXRα gene stably transfected cell line for RXRα protein stable high expression and a preparation method therefor. The cell line is a cell clone where the RXRα gene is randomly integrated into the chromosomes of an SK-N-SH cell, and the genotype for the cell line is stable and can be stably inherited with cell proliferation, and the progeny cells stably and highly express the RXRα protein, thereby greatly improving the stability of the high expression of the RXRα protein to ensure the functionality of the RXRα gene and the reliability of the tested materials. Provided is a method for preparing the cell line, comprising the following steps: designing and preparing a eukaryotic expression vector, transfecting SK-N-SH cells with the eukaryotic expression vector by means of the nuclear transfection technique, obtaining the cell clones stably transfected with the RXRα gene by means of G418 drug screening, identifying whether the cell clone is a positive cell clone that is stably transfected with the RXRα gene by means of PCR and gene sequencing, identifying the expression level of the RXRα protein in positive cloned cells by using Western blot, and then obtaining the cell line for RXRα protein stable high expression in which genotypes can be stably passaged.

IPC Classes  ?

• C12N 15/09 - Recombinant DNA-technology
• C12N 5/079 - Neural cells
• C12N 5/0797 - Stem cellsProgenitor cells
• C12N 5/09 - Tumour cells
• C12N 5/22 - Human cells

Abstract

An RXRα gene knockout cell system with stable and low expression of RXRα protein. The cell system is an RXRα gene knockout cell system; the number of knockout bases is an integral multiple of a number except 3; RXRα protein expression in a cell is remarkably reduced, and the genotype is stable and can be passed to next generations. Also provided is a preparation method for the cell system: according to RXRα gene sequence information, designing a CRISPR knockout gRNA sequence, establishing a gRNA expression vector, and carrying out in-vitro cell level detection on gRNA shearing activity; then, using a nuclear transferring method to carry out cotransfection on an immortalized cell of human neuroblastoma, i.e., an SK-N-SH cell, by using cas9 and the gRNA expression vector; carrying out G418 medicine screening to obtain stable cell clone, carrying out a PCR and gene sequencing to identify that the cell colon is an RXRα gene knockout cell colon for knocking out an integral multiple of a number except 3 of gene, so that the RXRα gene knockout cell system with stable and low expression of the RXRα protein is obtained.

IPC Classes  ?

• C12N 5/10 - Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
• C12N 15/90 - Stable introduction of foreign DNA into chromosome