Abstract

A method is provided for resuming a bioprocessing system that is configured to manufacture cells for a cellular therapy. The method, performed by one or more processors and using the bioprocessing system, comprises: executing one or more first sub-state machines, of a plurality of sub-state machines for manufacturing the cells; storing, in an external system, a plurality of states; based on detecting an event, pausing the manufacturing of the cells; based on receiving user input indicating to resume the manufacturing of the cells, retrieving a saved state from the external system; comparing the saved state with the state machine to determine a sub-state machine, from the one or more first sub-state machines, that was most recently executed by the bioprocessing system prior to pausing the manufacturing of the cells; and resuming the manufacturing of the cells based on the determined sub-state machine.

IPC Classes  ?

• G16H 10/00 - ICT specially adapted for the handling or processing of patient-related medical or healthcare data
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

Methods and apparatus for mixer characterization are disclosed. An example apparatus includes interface circuitry, machine readable instructions, and programmable circuitry to at least one of instantiate or execute the machine readable instructions to identify a computational parameter to match a first set of input parameters to a first mixing time of the mixer, determine, based on the computational parameter, a second mixing time of the mixer based on a second set of input parameters, generate, based on the second mixing time, at least one of a time-associated recommendation or a location-associated recommendation, the time- associated recommendation or the location-associated recommendation including at least one of a time to reach homogeneity, a sequence of feed addition to the mixer, or a location of the feed addition to the mixer, and control at least one of the sequence of feed addition to the mixer or the location of the feed addition to the mixer.

IPC Classes  ?

• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Abstract

Provided herein is a mixer base assembly (200) comprising:(a) a body (501) having: an upper end (202) including a mating face (203) for mixing vessel (240) connection; a lower end (204) including a cavity; a fluid mixing chamber (212) having a bottom wall (205); a rounded side wall (220) and a flat side wall (210), wherein the rounded side wall (220) at a first part is the only wall encasing the fluid mixing chamber (212) and at a second part joins with the flat side wall (210) to encase the fluid mixing chamber (212); an inlet port (208, 207, 408, 411) arranged in one of the side walls (210, 401); an outlet port (207, 208, 406, 413) arranged in one of the side walls (210, 401), and; at least one probe port (209) arranged in one of side walls (210, 401); an impeller seat (230) arranged in the cavity (214, 235, 404, 412) in the lower end (204) of the body (120, 501); and, an impeller (236) arranged in the impeller seat (230).

IPC Classes  ?

• B01F 27/808 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with stirrers driven from the bottom of the receptacle
• B01F 27/81 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis the stirrers having central axial inflow and substantially radial outflow
• B01F 27/91 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with propellers
• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/42 - Clamping or holding arrangements for mounting receptacles on mixing devices
• B01F 35/43 - Supporting receptacles on frames or stands
• B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
• B01F 35/90 - Heating or cooling systems
• B01F 35/92 - Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners

Abstract

Provided herein is a jacketed mixing vessel housing, comprising: (a) three jacketed side walls (401, 402, 403), each of the three jacketed side walls connected to at least one of the other jacketed side walls, each of the jacketed side walls defining an interior cavity, wherein each of the interior cavities is in fluid connection with the others; (b) a base (410) supporting the three jacketed side walls at a bottom side of the three jacketed side walls; (c) an inlet port (408) on the bottom side of one of the jacketed side walls in fluid connection with the interior cavity; and (d) an outlet port (406) on one of the jacketed side walls in fluid connection with the interior cavity at a top side of the interior cavity; wherein the bottom side of the jacketed wall side wall containing the inlet slopes downwardly in a direction towards the inlet port, and wherein the three interior cavities of the three jacketed side walls are configured such that the three interior cavities are substantially filled by a fluid entering the jacketed mixing vessel housing before the fluid reaches the outlet port.

IPC Classes  ?

• B01F 27/808 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with stirrers driven from the bottom of the receptacle
• B01F 27/91 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with propellers
• B01F 35/43 - Supporting receptacles on frames or stands
• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/90 - Heating or cooling systems
• B01F 35/92 - Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means
• F28D 1/06 - Heat-exchange apparatus having stationary conduit assemblies for one heat-exchange medium only, the media being in contact with different sides of the conduit wall, in which the other heat-exchange medium is a large body of fluid, e.g. domestic or motor car radiators with the heat-exchange conduits forming part of, or being attached to, the tank containing the body of fluid

Abstract

A bioprocess mixer system (100), comprising: a mixer base assembly (200) comprising: a body having: an upper end (202) including a mating face (203) for mixing vessel (240) connection; a lower end (204) including a cavity (214); a plurality of side walls (210), the plurality of side walls (210 comprising a rounded side wall (220) and a flat side wall (210); an inlet port (208) arranged in one of the one or more side walls (210) an outlet port (207) arranged in one of the one or more side walls (210); at least one probe port (209) arranged in one of the one or more side walls (210); and, a fluid mixing chamber (212) having a bottom wall (205); an impeller seat (230) arranged in the cavity (214) in the lower end (204) of the body; and, an impeller (236) arranged in the impeller seat (230); and a mixer drive system (140) comprising: a drive system (140) configured to drive the impeller (236); and a housing (400) containing the drive system (140), the housing (400) comprising a locking mechanism configured to lock the mixer base assembly (200) into an aligned position when connected to the housing (400).

IPC Classes  ?

• B01F 27/808 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with stirrers driven from the bottom of the receptacle
• B01F 27/81 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis the stirrers having central axial inflow and substantially radial outflow
• B01F 27/91 - Mixers with rotary stirring devices in fixed receptaclesKneaders with stirrers rotating about a substantially vertical axis with propellers
• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/42 - Clamping or holding arrangements for mounting receptacles on mixing devices
• B01F 35/43 - Supporting receptacles on frames or stands
• B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
• B01F 35/90 - Heating or cooling systems
• B01F 35/92 - Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners

Abstract

The invention provides stabilizing agents, compositions, methods of manufacturing, and uses thereof. In one aspect, a lipid nanoparticle composition includes: (a) an ionizable lipid; (b) two or more lipids; and (c) a stabilizing agent of formula (I) or formula (II) and methods for preparing the stabilizing agents and the lipid nanoparticle are provided.

IPC Classes  ?

• A61K 9/51 - Nanocapsules
• A61K 38/00 - Medicinal preparations containing peptides
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• C07C 15/00 - Cyclic hydrocarbons containing only six-membered aromatic rings as cyclic part

Abstract

The invention provides stabilizing agents, compositions, methods of manufacturing, and uses thereof. In one aspect, a lipid nanoparticle composition includes: (a) an ionizable lipid; (b) two or more lipids; and (c) a stabilizing agent of formula (I) and a method for preparing the stabilizing agent and the lipid nanoparticle are provided.

IPC Classes  ?

• A61K 9/51 - Nanocapsules
• A61K 38/00 - Medicinal preparations containing peptides
• A61K 39/00 - Medicinal preparations containing antigens or antibodies
• C07C 15/00 - Cyclic hydrocarbons containing only six-membered aromatic rings as cyclic part

Abstract

A method for preparing nucleic acid-containing lipid nanoparticles (NALNP) from a nucleic acid-containing neutral pH ionic salt solution and a lipid solution is provided. The method includes combining the nucleic acid-containing neutral pH ionic salt solution and the lipid solution and forming a combined mixture. The method further includes introducing an aqueous solution of lower ionic concentration than that of the neutral pH ionic salt solution into the combined mixture, thereby creating an ionic flux and forming the nucleic acid-containing lipid nanoparticles (NALNP).

IPC Classes  ?

• A61K 9/51 - Nanocapsules
• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy

Abstract

The present invention relates to methods and compositions for the dissociation of biological tissue with the aim of obtaining single cells for analysis. More particularly, the compositions of the present invention are enzyme compositions comprising proteinase K. The inventive compositions can be used in methods that effectively dissociate tissue at lower temperatures for application in high-throughput single cell omics research.

IPC Classes  ?

• C12N 9/22 - Ribonucleases
• C12N 9/50 - Proteinases

Abstract

The present invention involves a stoppering apparatus for closing a plurality of pharmaceutical containers held in a container nest with a corresponding plurality of closures held in a closure nest. The apparatus comprises a controlled environment enclosure capable of maintaining an aseptic condition. Within the enclosure, an actuated ram comprising an upward facing horizontal ram surface is configured to move along a vertical path. A fixed plate is disposed within the controlled environment enclosure above the ram and comprises a lower horizontal surface parallel to and facing the horizontal ram surface. Elastically compliant elements may be attached to the upward facing horizontal ram surface to be compressible between the containers and the horizontal ram surface. Elastically compliant vertical pins may be attached to the fixed plate to engage with the closure tops under the action of the actuated ram. The elastically compliant elements compensate for the variation in height of the containers.

IPC Classes  ?

• B65B 3/00 - Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans or jars
• B65B 7/28 - Closing semi-rigid or rigid containers or receptacles not deformed by, or not taking-up shape of, contents, e.g. boxes or cartons by applying separate preformed closures, e.g. lids, covers

Abstract

The present invention provides for simple and rapid filtering of biological samples, whereby a sample can be analyzed in the same device or a different device. In one preferred example, a membrane filter 12 that is particularly useful for the filtration of samples comprising viruses along with other biological materials that need be separated from the viruses is used. A lateral flow device 10 incorporating a filter membrane/membrane filter 12 is also disclosed.

IPC Classes  ?

• B01D 63/08 - Flat membrane modules
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 69/12 - Composite membranesUltra-thin membranes
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

Porous liquid-filtering membranes are provided having a boundary region substantially surrounding the pore region and having greater tear resistance than the pore region.

IPC Classes  ?

• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 71/64 - PolyimidesPolyamide-imidesPolyester-imidesPolyamide acids or similar polyimide precursors

Abstract

In an embodiment, a bioprocessing method includes securing a vessel containing a fluid to a platform capable of pivoting about a first axis, heating the fluid in the vessel while the platform is in a static, substantially upright position to facilitate a first bioprocessing procedure within the vessel. The method further includes pivoting the platform and vessel about the first axis to create a rocking motion to facilitate a second bioprocessing procedure within the vessel.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• B01F 31/23 - Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 3/06 - Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12M 1/02 - Apparatus for enzymology or microbiology with agitation meansApparatus for enzymology or microbiology with heat exchange means

Abstract

A sparger assembly (100) for a bioprocessing system (10) includes a base (126) and a plurality of spargers (130) connected to the base, each sparger including a plurality of pores, the plurality of spargers each have a generally cylindrical shape. Each of the plurality of spargers includes a sidewall and a top, which define the cylindrical shape, the sidewall and the top each include a plurality of pores. The pores of the sidewall can be arranged around a circumference of the sidewall at an array of heights. Ridges may also be located on the sidewall above a respective array of pores.

IPC Classes  ?

• B01F 23/231 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids by bubbling
• B01F 23/233 - Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
• C12M 1/00 - Apparatus for enzymology or microbiology
• B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Abstract

An apparatus (30) for reducing a minimum working volume of a stirred tank (12) includes a body (32) having upper (46) and lower (48) surfaces, and an exterior profile shaped to closely fit within an interior of the stirred tank. The body including an interior portion (34) extending through the body (32) from the upper surface to the lower surface, the interior portion configured to receive and partially surround an impeller (200) within the stirred tank, while allowing rotation of the impeller to agitate a fluid in the vessel when in use. The body further including an opening in a side surface of the body, the opening extending into the interior portion and configured to facilitate removal of fluid (60) from the vessel when the stirred tank is in use, and to be removed from the stirred tank via a drain.

IPC Classes  ?

• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

A vessel (24) includes an interior volume (25) containing a liquid, a spiral shaft (28) located within the interior volume, and a rotatable impeller (30). The impeller has an aperture (38) that receives the spiral shaft and allows the impeller to travel bidirectionally along the spiral shaft. When the impeller travels along the spiral shaft, the impeller rotates axially about the spiral shaft to agitate a liquid in the interior volume.

IPC Classes  ?

• B01F 27/07 - Stirrers characterised by their mounting on the shaft
• B01F 31/40 - Mixers with shaking, oscillating, or vibrating mechanisms with an axially oscillating rotary stirrer
• B01F 33/453 - Magnetic mixersMixers with magnetically driven stirrers using supported or suspended stirring elements
• B01F 35/513 - Flexible receptacles, e.g. bags supported by rigid containers
• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
• B01F 101/44 - Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation

Goods & Services

Enzyme-linked immunosorbent assay kit, used to quantify and characterize proteins in process-derived samples harvested from host cells; chemical preparations for scientific purposes, other than for medical or veterinary use; chemical preparations for analyses in laboratories, other than for medical or veterinary purposes; chemical substances for analyses in laboratories, other than for medical or veterinary purposes; chemical reagents, other than for medical or veterinary purposes; diagnostic reagents and preparations, except for medical or veterinary use.

Goods & Services

Enzyme-linked immunosorbent assay kit comprised of assays for research purposes used to quantify and characterize proteins in process-derived samples harvested from host cells; chemical preparations for scientific purposes, other than for medical or veterinary use; chemical preparations for analyses in laboratories, other than for medical or veterinary purposes; chemical substances for analyses in laboratories, other than for medical or veterinary purposes; chemical reagents, other than for medical or veterinary purposes; diagnostic reagents and preparations, except for medical or veterinary use

Goods & Services

Enzyme-linked immunosorbent assay kit, used to quantify and characterize proteins in process-derived samples harvested from host cells; chemical preparations for scientific purposes, other than for medical or veterinary use; chemical preparations for analyses in laboratories, other than for medical or veterinary purposes; chemical substances for analyses in laboratories, other than for medical or veterinary purposes; chemical reagents, other than for medical or veterinary purposes; diagnostic reagents and preparations, except for medical or veterinary use.

Goods & Services

Laboratory scientific apparatus, including holder and rack systems.

Goods & Services

Chemical products, preparations and substances for use in science and industry; papers (chemically treated or containing chemicals); test papers; sensitised papers; indicator papers; modified and chemically treated glass in fibre form; silica (chemically treated); cellulose powders; chemically treated cellulose; ion-exchange materials; buffer solutions; test papers, impregnated with chemical or compounds; all for filtration, separation, analysis and testing in laboratories, industry, environmental and medical research and research. Sterilising preparations; filtering preparations, materials and media for pharmaceutical, medical and sanitary purposes; air purifying preparations; filter capsules for pharmaceutical purposes; diagnostic preparations for medical purposes, diagnostic test kits for medical purposes; filters for the treatment, separation, or decontamination of gas, air or liquids for pharmaceutical purposes; chemical reagents for medical purposes; bacterial and biological preparations and cultures for medical use; blood plasma separating filters; cellulose for pharmaceutical purposes; chemico-pharmaceutical preparations. Scientific and laboratory apparatus and instruments; measuring, weighing, monitoring and checking apparatus and instruments; apparatus for chemical separation; filters, filter units and filter tubes and cartridges; apparatus for chemical separation; chromatography columns and equipment; separating funnels; soxhlet thimbles made from organic or in organic fibres; extraction symbols for use in chemical analysis being laboratory apparatus and instruments; diagnostic testing apparatus (not for medical purposes); stills for laboratory experiments; membranes for gas separators; cellulose nitrate membranes; media and membranes for storage of samples; pipette controllers; hot plate stirrers; chromatography papers; all for use for laboratories, industry, research, education and environmental testing. Medical, dental and veterinary apparatus and instruments; filtering materials for medical use; filter membranes, pharmaceutical grade aqueous filters, sterile vent filters, filters for use in blood and plasma separation, inline gas or air filters used in respiratory filtration, filter capsules, molecular sieves, IV filters, encapsulated filter devices, pre-filters, multi layer filters, disposable filtration capsules, microporous membranes, hydrophilic membranes, hydrophobic membranes, hydrophobic filters, glass microfibre filters, filter cartridges, pleated filter cartridges, radiation sterilizable filtering materials, microaggregate transfusion filters, filter housings; tubing sets for insufflation; ribbed or non-ribbed tubing; drain assemblies (tubing); connectors for filters and tubing, luer locks, barbs, sealing rings, clamps; all the aforesaid for medical, dental, veterinary or scientific purposes; filter capsules for medical purposes; filters for the treatment, separation, or decontamination of gas, air or liquids for medical purposes; blood plasma separating filters. Paper and paper articles; filter papers, chemically treated papers for filtering purposes, polyethylene and polythene backed papers, lens cleaning papers, drawing papers, writing papers, printmaking papers, test papers, not impregnated with chemical or compounds, blotting papers, carbon paper, electrocardiograph paper, paper sheets, paper rolls; bags [envelopes, pouches] of paper or plastics, for packaging; packaging containers of paper or cardboard; blotters.

Abstract

Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/67 - General methods for enhancing the expression

Abstract

Disclosed is a method for monitoring cell density during cell expansion resulting from a cell culture process in a bioreactor comprising the steps of: a) cultivating cells in a bioreactor culture chamber according to a cell culture process having cell culture parameters; b) during said process, introducing cell culture fluid inputs and generating waste materials; c) determining the intensity of volatile organic compounds (VOCs) and their chemical species in the waste materials; and d) estimating the density or population of cells in the bioreactor based on said determination.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters
• C12N 5/075 - OocytesOogonia
• C12N 5/0783 - T cellsNK cellsProgenitors of T or NK cells
• G01N 27/62 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosolsInvestigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electric discharges, e.g. emission of cathode
• G01N 30/02 - Column chromatography
• G01N 33/00 - Investigating or analysing materials by specific methods not covered by groups
• G01N 33/483 - Physical analysis of biological material

Abstract

Provided is a membrane transfer cassette for electroblotting, the cassette comprising a first support panel and a second support panel, at least a region of one or each of the first and second support panels having an interlocking pattern of polygonal apertures. The membrane transfer cassette is particularly useful when electroblotting with large format gels as the design of the cassette increases the exposed surface area between the gel and the membrane while also enhancing the strength of the cassette to support large gels but is also advantageous for any size of gel.

IPC Classes  ?

• B01D 57/02 - Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. by electrophoresis
• G01N 27/447 - Systems using electrophoresis

Abstract

The present invention provides for simple and rapid filtering of biological samples, whereby a sample can be analyzed in the same device or a different device. In one preferred example, a membrane filter (12) that is particularly useful for the filtration of samples comprising viruses along with other biological materials that need be separated from the viruses is used. A lateral flow device (10) incorporating a filter membrane/membrane filter (12) is also disclosed.

IPC Classes  ?

• B01D 63/08 - Flat membrane modules
• B01D 69/10 - Supported membranesMembrane supports
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• G01N 33/558 - ImmunoassayBiospecific binding assayMaterials therefor using diffusion or migration of antigen or antibody

Abstract

Methods and apparatus for scaling in bioprocess systems are disclosed. An example apparatus for bioprocess scaling includes at least one memory to store instructions, and processor circuitry to execute the instructions to identify at least one operating parameter of a target bioreactor, determine an upper boundary and/or a lower boundary defining a design space for at least one bioreactor process parameter to match at least one target parameter range based on the at least one operating parameter, simulate changes in the at least one target parameter range based on an adjustment to the upper boundary and/or the lower boundary in the design space, and configure the target bioreactor using output obtained from the adjustment to the upper boundary and/or the lower boundary to identify a match between the at least one target parameter range and a user-based input of a target bioprocess parameter value.

IPC Classes  ?

• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

Porous liquid-filtering membranes (102) are provided having a boundary region (303) substantially surrounding a pore region (302) and having greater tear resistance than the pore region (302). In various aspects, a liquid-filtering tear-resistant porous polymeric membrane (102) comprises a pore region (302) comprising a plurality of pores having a minimum dimension of less than (100) microns and a ring-shaped tear- resistant boundary region (303).

IPC Classes  ?

• B01D 61/14 - UltrafiltrationMicrofiltration
• B01D 67/00 - Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 71/64 - PolyimidesPolyamide-imidesPolyester-imidesPolyamide acids or similar polyimide precursors

Goods & Services

(1) Chemical products, preparations and substances for use in science and industry; papers (chemically treated or containing chemicals); test papers; sensitised papers; indicator papers; modified and chemically treated glass in fibre form; silica (chemically treated); cellulose powders; chemically treated cellulose; ion-exchange materials; buffer solutions; test papers, impregnated with chemical or compounds; all for filtration, separation, analysis and testing in laboratories, industry, environmental and medical research and research. (2) Sterilising preparations; filtering preparations, materials and media for pharmaceutical, medical and sanitary purposes; air purifying preparations; filter capsules for pharmaceutical purposes; diagnostic preparations for medical purposes, diagnostic test kits for medical purposes; filters for the treatment, separation, or decontamination of gas, air or liquids for pharmaceutical purposes; chemical reagents for medical purposes; bacterial and biological preparations and cultures for medical use; blood plasma separating filters; cellulose for pharmaceutical purposes; chemico-pharmaceutical preparations. (3) Scientific and laboratory apparatus and instruments; measuring, weighing, monitoring and checking apparatus and instruments; apparatus for chemical separation; filters, filter units and filter tubes and cartridges; apparatus for chemical separation; chromatography columns and equipment; separating funnels; soxhlet thimbles made from organic or in organic fibres; extraction symbols for use in chemical analysis being laboratory apparatus and instruments; diagnostic testing apparatus (not for medical purposes); stills for laboratory experiments; membranes for gas separators; cellulose nitrate membranes; media and membranes for storage of samples; pipette controllers; hot plate stirrers; chromatography papers; all for use for laboratories, industry, research, education and environmental testing. (4) Medical, dental and veterinary apparatus and instruments; filtering materials for medical use; filter membranes, pharmaceutical grade aqueous filters, sterile vent filters, filters for use in blood and plasma separation, inline gas or air filters used in respiratory filtration, filter capsules, molecular sieves, IV filters, encapsulated filter devices, pre-filters, multi layer filters, disposable filtration capsules, microporous membranes, hydrophilic membranes, hydrophobic membranes, hydrophobic filters, glass microfibre filters, filter cartridges, pleated filter cartridges, radiation sterilizable filtering materials, microaggregate transfusion filters, filter housings; tubing sets for insufflation; ribbed or non-ribbed tubing; drain assemblies (tubing); connectors for filters and tubing, luer locks, barbs, sealing rings, clamps; all the aforesaid for medical, dental, veterinary or scientific purposes; filter capsules for medical purposes; filters for the treatment, separation, or decontamination of gas, air or liquids for medical purposes; blood plasma separating filters. (5) Paper and paper articles; filter papers, chemically treated papers for filtering purposes, polyethylene and polythene backed papers, lens cleaning papers, drawing papers, writing papers, printmaking papers, test papers, not impregnated with chemical or compounds, blotting papers, carbon paper, electrocardiograph paper, paper sheets, paper rolls; bags [envelopes, pouches] of paper or plastics, for packaging; packaging containers of paper or cardboard; blotters.

Goods & Services

Chemicals, chemical preparations and chemical substances for use in science and industry, other than for medical or veterinary purposes; test paper, chemical test papers; sensitised papers being chemical test paper; indicator papers being chemical test paper; modified and chemically treated glass in fibre form in the nature of glass staining chemicals; chemical preparation in the nature of silica based density centrifugation media to be used in biochemical and clinical research, silica gel; cellulose powders being cellulose; chemically treated cellulose; buffer solutions for use in analytical chemistry; chemical test papers, namely, test papers, impregnated with chemical or compounds; all for filtration, separation, analysis and testing in laboratories, industry, environmental and medical research and research Sterilising preparations; filtering aids, materials and media for pharmaceutical, medical and sanitary purposes in the nature of biochemical reagents for medical purposes and chemical reagents for medical use for medical purposes; air purifying preparations; membrane filter capsules, sold empty, for pharmaceutical purposes; diagnostic preparations for medical purposes, diagnostic test kits for medical purposes comprised of medical diagnostic reagents and assays for testing body fluids; chemical reagents for medical purposes; bacterial preparations for medical use and cell therapy; biological preparations for the treatment of cancer and biological tissue cultures for medical use; bacterial preparation for medical use; blood plasma separating filters being blood plasma; cellulose ethers for pharmaceutical purposes; chemicopharmaceutical preparations for medical use for treating non-infectious and infectious diseases Scientific and laboratory apparatus and instruments, namely, pre-packed columns for use in separating DNA and RNA and purification apparatus for use in chemical separation, all for use for laboratories, industry, research, education and environmental testing; laboratory filters, filter units and filter tubes and cartridges, all for use for laboratories, industry, research, education and environmental testing; chromatography columns for laboratory use; laboratory equipment, namely, separating funnels, all for use for laboratories, industry, research, education and environmental testing; scientific laboratory apparatus in the nature of extraction thimbles made from organic or in organic fibres, all for use for laboratories, industry, research, education and environmental testing; extraction symbols for use in chemical analysis being laboratory apparatus and instruments, all for use for laboratories, industry, research, education and environmental testing; wipes and swabs for use in diagnostic testing, not for medical purposes; stills for laboratory experiments, all for use for laboratories, industry, research, education and environmental testing; scientific apparatus in the nature of membranes for gas separators for use in science and research industry; cellulose nitrate membranes in the nature of cellular mixed ester membranes being laboratory apparatus, all for use for laboratories, industry, research, education and environmental testing; media and membranes for storage of samples in the nature of cellular mixed ester membranes, all for use for laboratories, industry, research, education and testing; pipette controllers being pipette racks for laboratory use; hot plate glass stirrers, all for use for laboratories, industry, research, education and environmental testing; chromatography papers being chromatography apparatus, all for use for laboratories, industry, research, education and environmental testing. Medical, dental and veterinary apparatus and instruments, namely, filtration devices being filters for blood and blood components for medical purposes, air filters for medical ventilators, all for use for laboratories, industry, research, education and testing; filtering materials for medical use, namely, air filters for medical ventilators, blood filters, filters for anesthesia for use in laboratories, industry, research, education and testing; filters for medical purposes, namely, pharmaceutical grade aqueous medical blood filters, sterile vent filters for anesthesia, filters for use in blood and plasma separation, inline gas or air medical filters used in respiratory filtration, intravenous (IV) filters being intravenous stands, encapsulated filter devices for use in anesthesia, pre-filters for use in anesthesia,, multi layer filters for use in anesthesia,, microporous membranes, hydrophilic membranes, hydrophobic membranes, hydrophobic medical filters for tanks and containers, for monitoring airborne contaminants, and to protect vacuum pumps from water aspiration, glass microfibre filters, filter cartridges, pleated filter cartridges, radiation sterilizable filtering materials, microaggregate transfusion filters, filter housings, all for use for laboratories, industry, research, education and testing; blood tubing sets for insufflation; ribbed or non-ribbed tubing being medical tubing for drainage or transfusion; drainage tubes for medical purposes, all for use for laboratories, industry, research, education and testing; connectors for filters and tubing, luer locks, barbs, sealing rings, clamps in the nature of oxygen nasal cannula clamps for securing medical tubing; filter capsules being capsule endoscopes for medical purposes; filters for the treatment, separation, or decontamination of gas, air or liquids for medical purposes; medical device, namely, blood plasma separating filters, all for use for laboratories, industry, research, education and testing; filters for the treatment, separation, or decontamination of gas, air or liquids for pharmaceutical purposes Paper; filter papers, filter papers in the nature of chemically treated papers for filtering purposes, paper in the nature of polyethylene and polythene backed papers, paper in the nature of lens cleaning papers, drawing papers, writing papers, printmaking papers, paper in the nature of test papers, not impregnated with chemical or compounds, blotting papers, carbon paper, electrocardiograph paper

Abstract

A sampling system includes a graduated sampling chamber configured for fluid connection to a sample source, a pump device configured for fluid connection with the sampling chamber, and a sterile air filter intermediate the pump device and the sampling chamber, wherein the pump device is selectively actuatable to draw a volume of fluid from the sample source into the sampling chamber.

IPC Classes  ?

• C12M 1/26 - Inoculator or sampler
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers

Goods & Services

Laboratory scientific apparatus, namely, tube holders and racks

Abstract

A sterile foam breaking system (14, 42, 52) includes a foam collector (20) having an opening (26), configured to be disposed in a source (12) which generates foam. The sterile foam breaking system (14, 42, 52) further includes a non-contact type suction unit (23) coupled to the foam collector (20). The non-contact type suction unit (23) is configured to transfer the foam via the opening (26) of the foam collector (20) and break a portion of the foam to generate a first quantity of liquid droplets. The sterile foam breaking system (14, 42, 52) additionally includes a foam breaking unit (28, 44, 54) coupled to the non-contact type suction unit (23). The foam breaking unit (28, 44, 54) is configured to receive remaining portion of the foam and the first quantity of liquid droplets and break the remaining portion of the foam to generate a second quantity of liquid droplets.

IPC Classes  ?

• B01D 19/02 - Foam dispersion or prevention
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

The present invention relates to a sample processing bag (10) for use in a tissue disaggregation apparatus (200) for disaggregation of tissue therein. A clamp assembly 60 comprising a base support (70) and a clamp mechanism (72) is configured to retain the sample processing bag (10) adjacent to the base support (70) during a tissue disaggregation operation such that action of the tissue disaggregation apparatus 200 acts on the sample processing bag (10) to disaggregate any tissue therein.

IPC Classes  ?

• C12M 1/33 - Disintegrators
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

An apparatus (100) for managing gas bubbles (50) in a bioprocessing system (10) includes a body portion (110) having a underside surface (112), and an opening (114) in the body portion (110). The body portion (110) is configured for placement within a bioreactor vessel (12) such that the underside surface (112) of the body portion (110) is disposed in a liquid within the bioreactor vessel (12). The underside surface (112) of the body portion (110) is configured to divert rising gas bubbles (50) in the liquid towards the opening (114).

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

A biocompatible polymeric membrane includes pores (106) defined between two material layers, where the first membrane material layer (101) includes strips, and the second membrane material (104) binds to each of the plurality of first membrane material layer strips (101) includes a plurality of windows (105) exposing each of the first membrane material strips (101). The biocompatible polymeric filtration membrane comprises pores (106) defined by uniform passages defined by the first membrane material layer strips (101) and the second membrane material layer (104) within each window (105).

IPC Classes  ?

• B01D 69/02 - Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or propertiesManufacturing processes specially adapted therefor characterised by their properties
• B01D 69/12 - Composite membranesUltra-thin membranes
• B01D 71/64 - PolyimidesPolyamide-imidesPolyester-imidesPolyamide acids or similar polyimide precursors

Abstract

Methods and apparatus for bioprocess monitoring are disclosed. An example apparatus includes a controller to monitor a first bioprocess instrument, a data logger to collect data for the first bioprocess instrument, the collected data including data collected while the first bioprocess instrument is transferred from a first location to a second location, a configurator to configure the first bioprocess instrument to operate in a first mode at the first location and in a second mode at the second location, the first or second mode determined based on a type of processing at the first or second location, and a user interface to display the collected data to a user, the collected data including real-time bioprocess monitoring data, the controller to adjust a setting of the first bioprocess instrument based on the monitoring data, the monitoring data used to maintain controlled environmental conditions within a vessel of the instrument.

IPC Classes  ?

• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• G05B 15/00 - Systems controlled by a computer
• G06N 3/00 - Computing arrangements based on biological models
• G16B 40/00 - ICT specially adapted for biostatisticsICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• G16C 20/10 - Analysis or design of chemical reactions, syntheses or processes

Abstract

A mixing system (10) includes a container (20) for containing a fluid, and an agitator assembly (100) disposable in the container (20) for mixing the fluid. The agitator (100) includes an extensible shaft (110) and at least one impeller (116, 118) connected to the extensible shaft (110).

IPC Classes  ?

• B01F 7/00 - Mixers with rotary stirring devices in fixed receptacles; Kneaders
• B01F 13/08 - Magnetic mixers
• B01F 15/00 - Accessories for mixers
• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller

Abstract

The invention relates to the field of microcarrier perfusion culture of adherent cells. Specifically, the present invention relates to a high-density microcarrier retention device for perfusion culture of adherent cells, a microcarrier perfusion culture system for adherent cells containing the device, and methods of use thereof. The retention device of the present invention includes a sedimentation chamber, a pipeline connected to a bioreactor, a microcarrier retention filter membrane, a liquid backflushing device, an air backflushing device, a peristaltic pump and a pipeline connected to a receiver. The device has high efficiency in promoting the separation of microcarriers from cell culture medium and is helpful for perfusion culture of adherent cells and microcarriers. The retention device makes the culture volume in the bioreactor more flexible, can perform perfusion culture of 20%-100% of the maximum culture volume of the bioreactor, and the retention device can be linearly amplified according to the amplification of the bioreactor volume.

IPC Classes  ?

• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

A component management apparatus for a bioprocessing system includes a frame having a plurality of segments, including at least a first segment and a second segment pivotably connected to the first segment such that at least the second segment is movable between a closed position and an open position, and at least one mounting bracket connected to the frame for connection of a bioprocess component.

IPC Classes  ?

• C12M 3/00 - Tissue, human, animal or plant cell, or virus culture apparatus
• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

The bioprocessing perfusion system (10) includes a bioreactor (12) and a feed flow path (14). A first tangential flow filter (16) is coupled to the bioreactor (12) via the feed flow path (14) and a second tangential flow filter (18) is coupled to the bioreactor (12) via the feed flow path (14). The first tangential flow filter (16) is a microfiltration-type filter and the second tangential flow filter (18) is an ultrafiltration-type filter. The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to a receiving unit (58) via the permeate flow path (60). The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to the bioreactor (12) via the retentate flow path (46). A control unit (82) is communicatively coupled to the first feed control device (42), the second feed control device (44), the feed drive unit (40), the first permeate control device (64), the second permeate control device (66), the first retentate control device (48), and the second retentate control device (50).

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/36 - Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
• B01D 61/14 - UltrafiltrationMicrofiltration

Goods & Services

Laboratory equipment, namely, syringe filters for use with disposable reusable dispenser syringes; laboratory filters.

Goods & Services

(1) Laboratory equipment, namely, syringe filters for use with disposable, reusable dispenser syringes for scientific research in laboratories; laboratory filters, namely, syringe filters for scientific research in laboratories.

Goods & Services

Laboratory equipment, namely, syringe filters for use with disposable dispenser syringes and reusable dispenser syringes.

Abstract

A biological sample collector 500 comprising a solid support 12 having plural discrete areas (22,24,26,28 FIG. 1) for accepting a biological sample, each area being chemically differentiated, for example by having a different chemical treatment sorbed onto the solid support. An envelope 530 encloses the solid support. A cover portion 520 can cover the biological sample after collection to prevent infection. UV light can be applied to the collected sample to reduce the risk of infection.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 1/44 - Sample treatment involving radiation, e.g. heat

Abstract

The present invention relates to means for collection of biological samples. Specifically, the present invention provides a solid support for collection, storage and subsequent analysis of a biological sample as well as methods for use of the solid support of the invention and a kit comprising the solid support of the invention.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• G01N 1/02 - Devices for withdrawing samples
• G01N 1/10 - Devices for withdrawing samples in the liquid or fluent state

Abstract

A device (30) includes a base connector (32) having an opening (33) and an impeller connector (64) coupled to the base connector (32). The impeller connector (64) has a through-passage (66) aligned with the opening (33) of the base connector (32). Further, the device (30) includes a flexible tube (34) having a first end (36) and a second end (40), where the first end (36) of the flexible tube (34) is coupled to the impeller connector (64). Furthermore, the device (30) includes a seal component (38) and an impeller (42) coupled to the second end (40) of the flexible tube (34). Additionally, the device (34) includes an enclosure (46) disposed enclosing the impeller (42), the flexible tube (34), the impeller connector (64), and the base connector (32).

IPC Classes  ?

• B01F 7/16 - Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a vertical axis
• B01F 15/00 - Accessories for mixers
• B01F 15/06 - Heating or cooling systems
• B01F 3/04 - Mixing, e.g. dispersing, emulsifying, according to the phases to be mixed gases or vapours with liquids
• B01F 7/00 - Mixers with rotary stirring devices in fixed receptacles; Kneaders

Goods & Services

Laboratory equipment, namely, syringe filters for use with disposable reusable dispenser syringes; laboratory filters

Goods & Services

Laboratory equipment, namely, syringe filters for use with disposable reusable dispenser syringes; laboratory filters

Goods & Services

Laboratory equipment, namely, syringe filters for use with disposable reusable dispenser syringes; laboratory filters

Abstract

The present invention relates to a bioprocess bag (118) comprising a bag wall defining an enclosed volume for holding biomaterials. The bag wall comprises at least one inlet port (142) and at least one outlet port (146). The bioprocess bag (118) also comprises a tube structure (400) comprising a first opened-end proximate the bag wall and a second distal end, the tube extending into the enclosed volume, and the tube structure (400) comprising a reinforced portion (402) proximate the first opened-end.

IPC Classes  ?

• B01L 3/02 - BurettesPipettes
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12M 1/00 - Apparatus for enzymology or microbiology
• C12M 1/06 - Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
• C12M 3/04 - Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers

Abstract

Disclosed is a method for monitoring cell density during cell expansion resulting from a cell culture process in a bioreactor comprising the steps of: a) cultivating cells in a bioreactor culture chamber according to a cell culture process having cell culture parameters; b) during said process, introducing cell culture fluid inputs and generating waste materials; c) determining the intensity of volatile organic compounds (VOCs) and their chemical species in the waste materials; and d) estimating the density or population of cells in the bioreactor based on said determination.

IPC Classes  ?

• C12M 1/34 - Measuring or testing with condition measuring or sensing means, e.g. colony counters

Abstract

The present invention relates to a device and method for sample preparation and collection. More closely the invention relates to a device to isolate DNA, RNA and proteins or other biomolecules in one single step from the same undivided sample.

IPC Classes  ?

• C07K 1/34 - ExtractionSeparationPurification by filtration, ultrafiltration or reverse osmosis
• B01D 69/14 - Dynamic membranes
• C07K 1/36 - ExtractionSeparationPurification by a combination of two or more processes of different types
• G01N 33/538 - ImmunoassayBiospecific binding assayMaterials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a polyol, a serum albumin, a non-ionic surfactant and a reducing agent.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

The present invention relates to a device (10) for obtaining a sample (30) from a biological material (40) in solid form, said device comprising an array of micro-needles (30) arranged on a base plate (20). It further relates to a method for obtaining a sample (50) from a biological material (40) in solid form, comprising pressing the micro-needles (30) of the device (10) into said biological material (40), and subsequently removing the device from the biological material (40), and to the use of the device (10) in such a method.

IPC Classes  ?

• A61B 10/02 - Instruments for taking cell samples or for biopsy
• G01N 1/08 - Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
• A61M 37/00 - Other apparatus for introducing media into the bodyPercutany, i.e. introducing medicines into the body by diffusion through the skin

Goods & Services

laboratory equipment, namely, syringe filters for use with disposable reusable dispenser syringes

Goods & Services

(1) Laboratory equipment, namely syringe filters

Abstract

An electrospinning approach is disclosed for generating a dissolvable formulation of a reagent of interest in a nanoscale fiber medium. In one embodiment, the nanoscale fibers can incorporate and stabilize biological agents of interest, such as for storage at room temperature for extended periods. In one implementation, the fibers can be produced in a continuous manner and dissolve rapidly.

IPC Classes  ?

• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

A method for in vivo RNA or protein expression is provided. The method includes introducing a double-stranded concatemeric DNA into a eukaryotic cell to generate a desired RNA or protein. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences, wherein each of the plurality of tandem repeat sequences comprises an expression sequence. The double-stranded concatemeric DNA comprises one or more phosphorothioated nucleotides, wherein a ratio of phosphorothioated nucleotides to total nucleotides in the double-stranded concatemeric DNA is at least 1:1600. A eukaryotic cell comprising an exogeneous, double-stranded concatemeric DNA comprising the plurality of tandem repeat sequences is also provided.

IPC Classes  ?

• A61K 48/00 - Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseasesGene therapy
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/67 - General methods for enhancing the expression
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a) a polar aprotic solvent; b) a serum albumin; and optionally c) a non-ionic surfactant and/or a betaine.

IPC Classes  ?

• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/67 - General methods for enhancing the expression

Abstract

Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/μL to about 35 ng/μL. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

The present invention provides an indicator useful in confirming successful sterilisation and/or nucleic acid decontamination by ethylene oxide (EtO) treatment. The invention also provides a label comprising said indicator and methods for use of the indicator and label in a method of EtO treatment.

IPC Classes  ?

• A61L 2/28 - Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
• A61L 2/20 - Gaseous substances, e.g. vapours
• G01N 31/22 - Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroupsApparatus specially adapted for such methods using chemical indicators

Abstract

The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.

IPC Classes  ?

• B01L 7/00 - Heating or cooling apparatusHeat insulating devices
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6844 - Nucleic acid amplification reactions
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.

IPC Classes  ?

• G01N 1/44 - Sample treatment involving radiation, e.g. heat
• C08F 2/48 - Polymerisation initiated by wave energy or particle radiation by ultraviolet or visible light
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• C08F 8/50 - Partial depolymerisation
• G01N 1/02 - Devices for withdrawing samples

Abstract

A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

IPC Classes  ?

• B01D 57/02 - Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. by electrophoresis
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Goods & Services

(1) Laboratory reagents, namely, silica beads for scientific and research use

Goods & Services

(1) Chemical reagents and cell culture reagents for DNA and RNA sequencing for use in genomic research.

Goods & Services

Reagent kits for DNA and RNA sequencing for use in genomic research.

Goods & Services

Laboratory reagents, namely, silica beads for scientific and research use.

Goods & Services

Laboratory reagents, namely, silica beads for scientific and research use

Abstract

A method comprises: sorbing a sample solution comprising nucleic acids to a sample receiving portion of a quartz fiber filter by contacting the sample solution with the sample receiving portion; and washing the sample receiving portion while keeping most of nucleic acids around the sample receiving portion by flowing a wash solution through the sample receiving portion under a wicking force directed away from the sample receiving portion. An associated apparatus is also provided.

IPC Classes  ?

• C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01J 20/10 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
• B01J 20/28 - Solid sorbent compositions or filter aid compositionsSorbents for chromatographyProcesses for preparing, regenerating or reactivating thereof characterised by their form or physical properties
• B01J 20/281 - Sorbents specially adapted for preparative, analytical or investigative chromatography
• C07H 1/06 - SeparationPurification
• C07H 1/08 - SeparationPurification from natural products
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Abstract

The present techniques provide techniques for determining gene expression distinguishers of biological samples using expression data that comprises signal intensity of signal generators with binding specificity to target molecules. Multiple samples may be analyzed to determine gene expression distinguishers that may be used for identifying cell types or understanding the mechanism of disease progression.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G06F 19/24 - for machine learning, data mining or biostatistics, e.g. pattern finding, knowledge discovery, rule extraction, correlation, clustering or classification
• G01N 33/48 - Biological material, e.g. blood, urineHaemocytometers
• G06F 19/20 - for hybridisation or gene expression, e.g. microarrays, sequencing by hybridisation, normalisation, profiling, noise correction models, expression ratio estimation, probe design or probe optimisation

Abstract

The present invention relates to a method and kit for analyte detection. More precisely the invention relates to a method of detecting an analyte, comprising storing short single stranded nucleic acids (10-100 nt), such as aptamers and micro RNA, on a solid support at ambient temperature; and subsequent amplification of said nucleic acids for detection of analyte(s).

IPC Classes  ?

• C12N 15/115 - Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12Q 1/6869 - Methods for sequencing

Abstract

An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface; and an outlet for driving out the sample fluid. The device further comprises a third layer comprising an adhesive material such as a patterned adhesive material and a flow path, wherein the third layer is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer and the outlet is in contact with the flow path of the third layer for allowing the sample fluid out from the integrated device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• B01L 3/02 - BurettesPipettes

Abstract

Disclosed is a biological sample collector 500 comprising a solid support 12 having plural discrete areas (22, 24, 26, 28 FIG. 1) for accepting a biological sample, each area being chemically differentiated, for example by having a different chemical treatment sorbed onto the solid support. An envelope 530 encloses the solid support. A cover portion 520 can cover the biological sample after collection to prevent infection. UV light can be applied to the collected sample to reduce the risk of infection.

IPC Classes  ?

• G01N 1/28 - Preparing specimens for investigation
• G01N 1/00 - SamplingPreparing specimens for investigation
• G01N 1/44 - Sample treatment involving radiation, e.g. heat
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

IPC Classes  ?

• B01D 21/00 - Separation of suspended solid particles from liquids by sedimentation
• B01D 21/24 - Feed or discharge mechanisms for settling tanks
• G01N 33/49 - Physical analysis of biological material of liquid biological material blood
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• G01N 1/40 - Concentrating samples
• C12M 1/12 - Apparatus for enzymology or microbiology with sterilisation, filtration, or dialysis means
• C12M 1/26 - Inoculator or sampler
• B01D 17/02 - Separation of non-miscible liquids
• B01D 21/02 - Settling tanks
• C12M 1/00 - Apparatus for enzymology or microbiology
• C02F 1/48 - Treatment of water, waste water, or sewage with magnetic or electric fields
• C02F 1/44 - Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
• C02F 101/32 - Hydrocarbons, e.g. oil

Abstract

The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.

IPC Classes  ?

• G01N 27/30 - Electrodes, e.g. test electrodesHalf-cells
• C12M 1/00 - Apparatus for enzymology or microbiology
• H01G 9/042 - Electrodes characterised by the material
• H01G 9/22 - Devices using combined reduction and oxidation, e.g. redox arrangement or solion
• H01G 11/02 - Hybrid capacitors, i.e. capacitors having different positive and negative electrodesElectric double-layer [EDL] capacitorsProcesses for the manufacture thereof or of parts thereof using combined reduction-oxidation reactions, e.g. redox arrangement or solion
• H01G 11/28 - Electrodes characterised by their structure, e.g. multi-layered, porosity or surface features arranged or disposed on a current collectorLayers or phases between electrodes and current collectors, e.g. adhesives
• H01G 11/48 - Conductive polymers
• H01G 11/86 - Processes for the manufacture of hybrid or EDL capacitors, or components thereof specially adapted for electrodes
• G01N 27/453 - Cells therefor
• H01M 4/60 - Selection of substances as active materials, active masses, active liquids of organic compounds

Abstract

The invention relates to devices, methods and systems for collecting and solidifying waste material from bioreactor cell cultures in order to facilitate waste disposal. The invention finds particular utility in mammalian cell culture applications. A transverse wall divides the interior of a waste bag into two chambers, the first chamber containing an inlet receiving waste liquid from a bioreactor, the second chamber containing an absorbent material to solidify the liquid into a gel. The transverse wall acts to direct the flow of waste media into the second chamber where it is converted into a gel and further prevents the inlet from being blocked by any gel or particulate materials. Methods and uses regarding this invention are described.

IPC Classes  ?

• C12M 1/00 - Apparatus for enzymology or microbiology

Abstract

The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a polar aprotic solvent; a serum albumin, and a polyol.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C07H 1/00 - Processes for the preparation of sugar derivatives
• C07H 21/02 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• C12Q 1/6848 - Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.

IPC Classes  ?

• C12P 21/00 - Preparation of peptides or proteins
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/67 - General methods for enhancing the expression

Abstract

An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12Q 1/6897 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Embodiments of the invention provides a method for detection of at least one analyte derived from a sample, comprising the steps of: a) depositing the sample on a surface of a solid support; b) transferring at least a portion of the solid support to a receptacle suitable for performing a specific binding assay for one or more analytes of interest; c) optionally washing the portion; d) adding a single specific binding partner for each analyte of interest to the receptacle, the binding partner being labelled with an oligonucleotide sequence; e) mixing the portion with nucleic acid amplification reagents; f) amplifying the oligonucleotide sequence; and g) detecting amplified nucleic acid. The invention also provides a kit for use with the method for detection of at least one analyte derived from a sample.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• G01N 33/50 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing
• G01N 33/53 - ImmunoassayBiospecific binding assayMaterials therefor
• C12Q 1/6804 - Nucleic acid analysis using immunogens
• G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12Q 1/6851 - Quantitative amplification

Abstract

The present invention relates to biomolecule stabilization to provide biomolecules, such as sensitive polymerases, in a convenient ready-to-go format. The invention provides a method and composition in which non-ionic surfactant or detergents of the polyoxyethylene cetyl ether family are used, preferably a Brij reagent or a combination of Brij reagents.

IPC Classes  ?

• C12N 9/96 - Stabilising an enzyme by forming an adduct or a compositionForming enzyme conjugates
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12Q 1/686 - Polymerase chain reaction [PCR]

Abstract

Disclosed is a method for detecting and quantifying gut flora derived from human faeces, comprising acquiring a solid support containing human faeces; optionally amplifying nucleic acid from the human faeces; and detecting and quantifying the presence of gut flora of interest. Also disclosed is a method for assessing whether a person has type-2 diabetes as well as a kit for detecting and quantifying gut flora from human faeces, comprising a solid support for collecting human faeces; and primer pairs for amplifying 16S rRNA sequence from bacteria of interest.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/00 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions
• C12Q 1/10 - Enterobacteria
• C12Q 1/689 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
• C12Q 1/6883 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
• C12Q 1/06 - Quantitative determination
• A61K 38/00 - Medicinal preparations containing peptides
• C12Q 1/6827 - Hybridisation assays for detection of mutation or polymorphism

Abstract

Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6844 - Nucleic acid amplification reactions
• B01L 7/00 - Heating or cooling apparatusHeat insulating devices

Abstract

An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.

IPC Classes  ?

• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12Q 1/6806 - Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
• A61B 5/15 - Devices for taking samples of blood
• G01N 1/14 - Suction devices, e.g. pumpsEjector devices
• B01L 3/02 - BurettesPipettes

Abstract

The present invention relates to compositions, methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. The dried reagent composition of the invention can be used for easy processing and amplification of nucleic acid samples.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

A data storage medium is disclosed comprising a solid support matrix including an optional stabilising reagent or reagents in a dry form, for use as a support for artificially synthesised oligonucleotide sequences encoded with data. Preferably the matrix is fibrous (for example cellulose, or glass, fibres) formed into a support of sufficient strength to hold the oligonucleotide sequences. The stabilising reagents are preferably a combination of a weak base, and a chelating agent, optionally, uric acid or a urate salt, and optionally an anionic surfactant.

IPC Classes  ?

• B01J 19/00 - Chemical, physical or physico-chemical processes in generalTheir relevant apparatus
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6874 - Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation [SBH]

Abstract

The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.

IPC Classes  ?

• C12N 7/00 - Viruses, e.g. bacteriophagesCompositions thereofPreparation or purification thereof
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/70 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving virus or bacteriophage

Abstract

m) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

IPC Classes  ?

• C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
• C12Q 1/6844 - Nucleic acid amplification reactions
• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

The present invention relates to the field of nucleic acid collection and storage, and in particular to the collection and long-term storage of nuclei. The invention provides a device and method which can be used to capture and store nuclei at ambient temperatures allowing the subsequent isolation of nucleic acids by passive washing with a wash buffer. The present invention also provides a kit including the device of the invention suitable for carrying out the method of the invention.

IPC Classes  ?

• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• B01L 3/00 - Containers or dishes for laboratory use, e.g. laboratory glasswareDroppers

Abstract

m) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.

IPC Classes  ?

• C12P 19/34 - Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• C12Q 1/6844 - Nucleic acid amplification reactions