Abstract

The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
• C12Q 1/6841 - In situ hybridisation

Abstract

The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
• C12Q 1/6841 - In situ hybridisation

Abstract

The present application relates to hybridization chain reaction (HCR). In particular, compositions and methods are presented for ultrasensitive molecular detection using HCR signal amplification. Some embodiments and methods involve cooperative probe junctions, reporter-labeled probes, and nonlinear HCR signal amplification.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6818 - Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
• C12Q 1/6841 - In situ hybridisation

Goods & Services

Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; kits comprised of reagents and assays for scientific and research use; kits comprised primarily of biological reagents for scientific and research purposes; diagnostic tests and test kits primarily composed of reagents for scientific or research purposes; reagents and assays for scientific and research use; diagnostic reagents and preparations, other than for veterinary use; protein assays and nucleotide assays for scientific and research use; nucleic acid sequences for scientific and research use; biological agents, reagents, assays, enzymes, nucleotides, buffers, chemical preparations, and biological preparations, all for scientific and research use. Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; kits comprised of diagnostic reagents and assays for medical and veterinary use; diagnostic test kits for medical use comprising reagents and assays; diagnostic kits comprised of reagents for medical purposes; reagents and assays for medical and veterinary use; diagnostic reagents and assays for medical and veterinary use; diagnostic protein assays and nucleotide assays for medical and veterinary use; diagnostic agents, reagents, assays, enzymes, nucleotides, and buffers, for medical and veterinary use; nucleic acid sequences for medical and veterinary use; medical preparations and substances. Retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits; online retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, and reagent and assay kits; administrative processing of subscription-based orders featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits. Scientific and technological services and research; scientific and medical research and development services; clinical research; laboratory research; laboratory testing services; research and development of new diagnostic tests, clinical products, diagnostic agents and assays; nucleic acid sequencing, screening, and analysis services for medical research purposes; design, custom design, and development of kits containing reagents commonly known as buffers, probes, and amplifiers, reagents, assays, and nucleic acid sequences, being chemical preparations for scientific research, medical diagnostics, and veterinary diagnostics use; product design services; information technology [IT] support services; technical support services, namely, providing technical advice and information relating to reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits (terms too vague in the opinion of the International Bureau – Rule 13 (2) (b) of the Regulations); nucleic acid sequence design and analysis services for scientific research purposes; medical research services. Medical services; medical diagnostic testing services; medical analysis services for diagnostic and treatment purposes; nucleic acid sequencing analysis services for medical diagnostic and treatment purposes.

Goods & Services

Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; Kits comprised of reagents and assays for scientific and research use; Kits comprised primarily of biological reagents and comprised secondarily of written protocol instructions for use thereof for scientific and research purposes; Diagnostic tests and test kits primarily composed of reagents for scientific or research purposes; Reagents and assays for scientific and research use; Diagnostic reagents and preparations, other than for veterinary use; Protein assays and nucleotide assays for scientific and research use; Nucleic acid sequences for scientific and research use; Biological agents, reagents, assays, enzymes, nucleotides, buffers, chemical preparations, and biological preparations, all for scientific and research use.. Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; Kits comprised of diagnostic reagents and assays for medical and veterinary use; Diagnostic test kits for medical use comprising reagents and assays; Diagnostic kits containing reagents and a written instructional protocol for use thereof, for medical purposes; Reagents and assays for medical and veterinary use; Diagnostic reagents and assays for medical and veterinary use; Diagnostic protein assays and nucleotide assays for medical and veterinary use; Diagnostic agents, reagents, assays, enzymes, nucleotides, and buffers, for medical and veterinary use; Nucleic acid sequences for medical and veterinary use; Medical preparations and substances.. Retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits; Online retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, and reagent and assay kits; Subscription-based order fulfillment services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits.. Scientific and technological services and research; Scientific and medical research and development services; Clinical research; Laboratory research; Laboratory testing services; Research and development of new diagnostic tests, clinical products, diagnostic agents and assays; Nucleic acid sequencing, screening, and analysis services for medical research purposes; Design, custom design, and development of kits containing reagents commonly known as buffers, probes, and amplifiers, reagents, assays, and nucleic acid sequences, being chemical preparations for scientific research, medical diagnostics, and veterinary diagnostics use; Product design services; Technical support services; Nucleic acid sequence design and analysis services for scientific research purposes; Medical research services; Nucleic acid sequencing design services for medical diagnostic and treatment purposes. Medical services; Medical diagnostic testing services; Medical analysis services for diagnostic and treatment purposes; Nucleic acid sequencing analysis services for medical diagnostic and treatment purposes.

Goods & Services

(1) Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; kits comprised of reagents and assays for scientific and research use; kits comprised primarily of biological reagents for scientific and research purposes; diagnostic tests and test kits primarily composed of reagents for scientific or research purposes; reagents and assays for scientific and research use; diagnostic reagents and preparations, other than for veterinary use; protein assays and nucleotide assays for scientific and research use; nucleic acid sequences for scientific and research use; biological agents, reagents, assays, enzymes, nucleotides, buffers, chemical preparations, and biological preparations, all for scientific and research use. (2) Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; kits comprised of diagnostic reagents and assays for medical and veterinary use; diagnostic test kits for medical use comprising reagents and assays; diagnostic kits comprised of reagents for medical purposes; reagents and assays for medical and veterinary use; diagnostic reagents and assays for medical and veterinary use; diagnostic protein assays and nucleotide assays for medical and veterinary use; diagnostic agents, reagents, assays, enzymes, nucleotides, and buffers, for medical and veterinary use; nucleic acid sequences for medical and veterinary use; medical preparations and substances. (1) Retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits; online retail store services featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, and reagent and assay kits; administrative processing of subscription-based orders featuring reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits. (2) Scientific and technological services and research; scientific and medical research and development services; clinical research; laboratory research; laboratory testing services; research and development of new diagnostic tests, clinical products, diagnostic agents and assays; nucleic acid sequencing, screening, and analysis services for medical research purposes; design, custom design, and development of kits containing reagents commonly known as buffers, probes, and amplifiers, reagents, assays, and nucleic acid sequences, being chemical preparations for scientific research, medical diagnostics, and veterinary diagnostics use; product design services; information technology [IT] support services; technical support services, namely, providing technical advice and information relating to reagents, assays, nucleotides, biological preparations, chemical preparations, diagnostic kits, reagent and assay kits (terms too vague in the opinion of the International Bureau - Rule 13 (2) (b) of the Regulations); nucleic acid sequence design and analysis services for scientific research purposes; medical research services. (3) Medical services; medical diagnostic testing services; medical analysis services for diagnostic and treatment purposes; nucleic acid sequencing analysis services for medical diagnostic and treatment purposes.

Abstract

Methods of analysis of a sample using hybridization chain reaction (HCR) are provided herein. Some embodiments involve one, two, or all three of the following aspects: 1) repeated signal detection, 2) overlapping binding sites, and 3) catalytic reporter deposition (CARD). Compositions and kits relating to these are also provided. Some embodiments encompass a method for repeated signal detection with reporter-labeled HCR hairpins involving providing a sample possibly containing one or more targets as well as possibly other molecules that are not targets, providing one or more probe sets each comprising either: a) one or more HCR initiator-labeled probes, or b) one or more probe units each comprising two or more HCR fractional initiator probes, providing one or more HCR amplifiers (each labeled with one or more reporters), detecting one or more signals from one or more reporters. In some embodiments, a probe unit comprises two or more HCR fractional initiator probes, wherein an HCR fractional initiator probe comprises a target-binding region and a fractional initiator, wherein the target-binding regions within a probe unit are configured to bind to overlapping or non-overlapping binding sites on the target, and wherein the fractional initiators on the probes within each probe unit are configured to bind to overlapping or non-overlapping binding sites on an HCR hairpin. Some embodiments encompass a method for HCR-mediated catalytic reporter deposition (CARD) for signal detection with hapten-labeled HCR hairpins involving providing a sample possibly containing one or more targets as well as possibly other molecules that are not targets, providing one or more probe sets each comprising either: a) one or more HCR initiator-labeled probes, or b) one or more probe units each comprising two or more HCR fractional initiator probes, providing one or more HCR amplifiers (each labeled with one or more haptens), providing one or more anti-haptens labeled with one or more reporter entities, wherein the reporter entity is an enzyme that mediates CARD, providing one or more CARD-substrates leading to deposition of one or more reporters, and detecting one or more signals from one or more reporters.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6825 - Nucleic acid detection involving sensors
• C12Q 1/6841 - In situ hybridisation

Goods & Services

Kits consisting primarily of reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; Kits comprised of reagents and assays for scientific and research use; Kits comprised primarily of biological reagents and also including written protocol instructions for scientific and research purposes; Diagnostic tests and test kits primarily composed of reagents for scientific or research purposes; Reagents and assays for scientific and research use; Diagnostic reagents and preparations, other than for medical or veterinary use; Protein assays and nucleotide assays for scientific and research use; Nucleic acid sequences for scientific and research use; Biological agents, reagents, assays, enzymes, nucleotides, buffers, chemical preparations, and biological preparations, all for scientific and research use Kits consisting primarily of diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; Kits comprised of diagnostic reagents and assays for medical and veterinary use, namely, reagents and assays for testing body fluids and tissues; Kits comprised of diagnostic reagents and assays for medical and veterinary use, namely, reagents and assays for testing body fluids and tissues for use in disease testing; Kits comprised of diagnostic reagents and assays for medical and veterinary use, namely, reagents and assays for detecting biomolecules, such as DNA, RNA, or protein, in body fluids and tissues; Diagnostic kits consisting primarily of reagents and also containing a written instructional protocol for medical purposes; Diagnostic protein assays and nucleotide assays for medical and veterinary use; Nucleic acid sequences for medical and veterinary use Retail store services featuring reagents, assays, nucleotides, biological preparations for use in industry and science, chemical preparations for scientific purposes, chemical preparations for pharmaceutical or medical purposes, namely, for testing body fluids and tissues, diagnostic kits, reagent and assay kits; Online retail store services featuring reagents, assays, nucleotides, biological preparations for use in industry and science, chemical preparations for scientific purposes, chemical preparations for pharmaceutical or medical purposes, namely, for testing body fluids and tissues, diagnostic kits, and reagent and assay kits; Subscription-based order fulfillment services featuring reagents, assays, nucleotides, biological preparations for use in industry and science, chemical preparations for scientific purposes, chemical preparations for pharmaceutical or medical purposes, namely, for testing body fluids and tissues, diagnostic kits, reagent and assay kits Scientific research; Scientific and technological services, namely, scientific research, analysis, and testing in the medical, veterinary, and agricultural fields; Scientific and technological services, namely, scientific research, analysis, and testing in the field of reagents, assays, and nucleotides for scientific, medical, and agricultural use; Scientific research and development; Medical research; Research and development in the medical, veterinary, and agricultural fields; Research and development of technology in the field of nucleotides for scientific, medical, veterinary, and agricultural purposes; Clinical research in the field of genetics and skin disease prevention; Providing laboratory research services for research validation in the field of gene expression; DNA, RNA, and protein testing services for scientific research purposes; Research and development of new products for others, namely, diagnostic tests, clinical products, diagnostic agents and assays; Nucleic acid sequencing, screening, and analysis services for medical research purposes; Design, custom design, and development of kits containing reagents commonly known as buffers, probes, and amplifiers, reagents, assays, and nucleic acid sequences, being chemical preparations for scientific research, medical diagnostics, and veterinary diagnostics use; New product design services; Nucleic acid sequence design and analysis services for scientific research purposes; Medical research services; none of the foregoing related to the field of robotics; Medical research services in the field of genetics and skin disease prevention Medical services; Medical diagnostic testing services; Medical analysis services for diagnostic purposes and treatment decision and selection; Nucleic acid sequencing analysis services for medical diagnostic purposes and treatment decision and selection

Abstract

Methods of analysis of a sample using hybridization chain reaction (HCR) are provided herein. Some embodiments involve one, two, or all three of the following aspects: 1) repeated signal detection, 2) overlapping binding sites, and 3) catalytic reporter deposition (CARD). Compositions and kits relating to these are also provided. Compositions and kits relating to these are also provided.

IPC Classes  ?

• C12Q 1/682 - Signal amplification
• C12Q 1/6816 - Hybridisation assays characterised by the detection means
• C12Q 1/686 - Polymerase chain reaction [PCR]
• C12N 15/11 - DNA or RNA fragmentsModified forms thereof

Goods & Services

Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; protein assays and nucleotide assays for scientific and research use; kits comprised of reagents and assays for scientific and research use; reagents and assays for scientific and research use; nucleic acid sequences for scientific and research use. Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; diagnostic protein assays and nucleotide assays for medical and veterinary use; kits comprised of diagnostic reagents and assays for medical and veterinary use; diagnostic reagents and assays for medical and veterinary use; nucleic acid sequences for medical and veterinary use.

Goods & Services

Scientific, medical and veterinary laboratory services, namely, nucleic acid sequence design and analysis services for agricultural, horticulture, veterinary, and husbandry purposes.

Goods & Services

Kits containing buffers, probes, and amplifiers for scientific and research use; Protein assays and nucleotide assays for scientific and research use; Kits containing reagents and assays for scientific and research use; Reagents and assays for scientific and research use; Nucleic acid sequences for scientific and research use; Diagnostic reagents and preparations, other than for medical or veterinary use; Chemical preparations for scientific and research use. Kits containing buffers, probes, and amplifiers for medical and veterinary use; Protein assays and nucleotide assays for medical and veterinary use; Kits containing reagents and assays for medical and veterinary use; Reagents and assays for medical and veterinary use; Nucleic acid sequences for medical and veterinary use; Diagnostic reagents and preparations for medical and veterinary use; Chemical preparations for medical and veterinary use; Diagnostic preparations for medical and veterinary purposes. Scientific and technological services and research; Medical research; DNA screening for scientific research purposes; Scientific research services for medical purposes; Scientific, medical, and veterinary laboratory services; Providing reagent sample testing services; Scientific consultancy, information and advisory services; Nucleic acid sequence design and analysis services. Medical services; Medical genetic design and analysis services for diagnostic, prognostic or treatment uses; Medical testing and analysis for diagnostic or treatment purposes; DNA, nucleic acid and genomic sequencing, screening, and analysis services for medical purposes; Genetic design and analysis services for medical purposes; Medical design and analysis services for diagnostic and treatment purposes; Medical diagnostic testing, monitoring and reporting service; Genome sequencing and analysis services for medical, agriculture, horticulture, veterinary, and husbandry purposes.

Goods & Services

(1) Kits containing reagents namely antibody buffers, probes, and amplifiers, being chemical preparations for use in the fields of biological and diagnostic testing for scientific and research use; laboratory reagents and assays kits for scientific and research use; diagnostic reagents and assays for medical-scientific research use; antibody reagents and assays for scientific and research use; chemical reagents and assays for scientific and research use, namely for bioluminescence; reagents and assays for scientific purposes for use in nucleic acid isolation and purification; naturally-occurring or synthetic nucleic acid sequences for medical-scientific research use. (2) Kits containing diagnostic chemical reagents namely buffers, probes, and amplifiers for medical and veterinary use; diagnostic protein assays and nucleotide assays for medical and veterinary use for diagnosing cancer; diagnostic protein assays and nucleotide assays for medical and veterinary use for detecting genetic predispositions; diagnostic protein assays and nucleotide assays for medical and veterinary use for testing of body fluids; diagnostic protein assays and nucleotide assays for medical and veterinary use for use in the diagnosis of pregnancy; diagnostic protein assays and nucleotide assays for medical and veterinary use for detecting COVID-19 and other coronavirus diseases; kits comprised of diagnostic reagents and assays for medical and veterinary use for diagnosing cancer; kits comprised of diagnostic reagents and assays for medical and veterinary use for detecting genetic predispositions; kits comprised of diagnostic reagents and assays for medical and veterinary use for testing of body fluids; kits comprised of diagnostic reagents and assays for medical and veterinary use for use in the diagnosis of pregnancy; kits comprised of diagnostic reagents and assays for medical and veterinary use for detecting COVID-19 and other coronavirus diseases; diagnostic reagents and assays for medical and veterinary use for diagnosing cancer; diagnostic reagents and assays for medical and veterinary use for detecting genetic predispositions; diagnostic reagents and assays for medical and veterinary use for testing of body fluids; diagnostic reagents and assays for medical and veterinary use for testing of body fluids; diagnostic reagents and assays for medical and veterinary use for use in the diagnosis of pregnancy; diagnostic reagents and assays for medical and veterinary use detecting COVID-19 and other coronavirus diseases; naturally-occurring or synthetic nucleic acid sequences for medical-scientific research use for medical and veterinary use for diagnosing cancer; naturally-occurring or synthetic nucleic acid sequences for medical-scientific and research use for medical and veterinary use for detecting genetic predispositions; naturally-occurring or synthetic nucleic acid sequences for medical-scientific and research use for medical and veterinary use for testing of body fluids; naturally-occurring or synthetic nucleic acid sequences for medical-scientific and research use for medical and veterinary use for use in the diagnosis of pregnancy; naturally-occurring or synthetic nucleic acid sequences for medical-scientific and research use for medical and veterinary use for detecting COVID-19 and other coronavirus diseases.

Goods & Services

(1) Scientific, medical and veterinary laboratory services, namely, nucleic acid sequence design and analysis services for agricultural, horticulture, veterinary, and husbandry purposes.

Goods & Services

Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; Protein assays and nucleotide assays for scientific and research use; Kits comprised of reagents and assays for scientific and research use; Reagents and assays for scientific and research use; Nucleic acid sequences for scientific and research use Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; Diagnostic protein assays and nucleotide assays for medical and veterinary use; Kits comprised of diagnostic reagents and assays for medical and veterinary use; Diagnostic reagents and assays for medical and veterinary use; Nucleic acid sequences for medical and veterinary use

Goods & Services

Kits containing reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for scientific and research use; Protein assays and nucleotide assays for scientific and research use; Kits comprised of reagents and assays for scientific and research use; Reagents and assays for scientific and research use; Nucleic acid sequences for scientific and research use Kits containing diagnostic reagents commonly known as buffers, probes, and amplifiers, being chemical preparations for medical and veterinary use; Diagnostic protein assays and nucleotide assays for medical and veterinary use; Kits comprised of diagnostic reagents and assays for medical and veterinary use; Diagnostic reagents and assays for medical and veterinary use; Nucleic acid sequences for medical and veterinary use

Goods & Services

Kits containing buffers, probes, and amplifiers, being chemical preparations for scientific and research use; Protein assays and nucleotide assays for scientific and research use; Kits comprised of reagents and assays for scientific and research use; Reagents and assays for scientific and research use; Nucleic acid sequences for scientific and research use Diagnostic kits containing buffers, probes, and amplifiers for medical and veterinary use; Protein assays and nucleotide assays for medical and veterinary use; Diagnostic kits comprised of reagents and assays for medical and veterinary use; Reagents and assays for medical and veterinary use; Nucleic acid sequences for medical and veterinary use

Goods & Services

Kits containing buffers, probes, and amplifiers, being chemical preparations for scientific and research use; protein assays and nucleotide assays for scientific and research use; kits comprised of reagents and assays for scientific and research use; reagents and assays for scientific and research use; nucleic acid sequences for scientific and research use Diagnostic kits containing buffers, probes, and amplifiers for medical and veterinary use; protein assays and nucleotide assays for medical and veterinary use; diagnostic kits comprised of reagents and assays for medical and veterinary use; reagents and assays for medical and veterinary use; nucleic acid sequences for medical and veterinary use

Goods & Services

Scientific, medical and veterinary laboratory services, namely, nucleic acid sequence design and analysis services for agricultural, horticulture, veterinary, and husbandry purposes

Abstract

Methods of analysis of a sample using hybridization chain reaction (HCR) are provided herein. Some embodiments involve one, two, or all three of the following aspects: 1) repeated signal detection, 2) overlapping binding sites, and 3) catalytic reporter deposition (CARD). Compositions and kits relating to these are also provided. Some embodiments encompass a method for repeated signal detection with reporter-labeled HCR hairpins involving providing a sample possibly containing one or more targets as well as possibly other molecules that are not targets, providing one or more probe sets each comprising either: a) one or more HCR initiator-labeled probes, or b) one or more probe units each comprising two or more HCR fractional initiator probes, providing one or more HCR amplifiers (each labeled with one or more reporters), detecting one or more signals from one or more reporters. In some embodiments, a probe unit comprises two or more HCR fractional initiator probes, wherein an HCR fractional initiator probe comprises a target-binding region and a fractional initiator, wherein the target-binding regions within a probe unit are configured to bind to overlapping or non-overlapping binding sites on the target, and wherein the fractional initiators on the probes within each probe unit are configured to bind to overlapping or non-overlapping binding sites on an HCR hairpin. Some embodiments encompass a method for HCR-mediated catalytic reporter deposition (CARD) for signal detection with hapten-labeled HCR hairpins involving providing a sample possibly containing one or more targets as well as possibly other molecules that are not targets, providing one or more probe sets each comprising either: a) one or more HCR initiator-labeled probes, or b) one or more probe units each comprising two or more HCR fractional initiator probes, providing one or more HCR amplifiers (each labeled with one or more haptens), providing one or more anti-haptens labeled with one or more reporter entities, wherein the reporter entity is an enzyme that mediates CARD, providing one or more CARD-substrates leading to deposition of one or more reporters, and detecting one or more signals from one or more reporters.

IPC Classes  ?

• C12N 15/11 - DNA or RNA fragmentsModified forms thereof
• C12Q 1/6816 - Hybridisation assays characterised by the detection means
• C12Q 1/682 - Signal amplification
• C12Q 1/686 - Polymerase chain reaction [PCR]