Compositions for expression of milk proteins in plants and co-expression of milk proteins and kinases in plants are disclosed herein. With respect to the disclosed composition herein, nucleic acids, which encode for the milk proteins and kinases, can be isolated. The milk proteins, which are expressed or co-expressed with kinases, in the plants, include: alpha S1 casein, alpha S2 casein, beta casein, and kappa casein. The kinases, which are co-expressed with the milk proteins, include: FAM20C and FAM20A.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
Compositions, methods and systems for phosphorylation of proteins in plants. One or more vectors are used to express at least one kinase and at least one payload protein in a plant cell, wherein the at least one payload protein is phosphorylated by the at least one kinase in the plant cell. For example, one or more vectors are used to co-express a recombinant kinase, a recombinant β-casein, and at least one of a recombinant κ-casein, recombinant αS1-casein, or a recombinant αS2-casein, wherein at least one of the recombinant caseins (e.g., the recombinant β-casein) is phosphorylated by the recombinant kinase in the plant cell.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
A23C 11/08 - Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing caseinates but no other milk proteins nor milk fats
A23J 1/00 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites
A23J 1/14 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seedsObtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from press-cake or oil-bearing seeds
Casein micelles can be extracted from transgenic soybeans by: breaking down plant seed containing casein micelles; producing a white flake; extracting casein micelles by mixing the flake with a liquid, thereby producing a supernatant; and separating the supernatant from the undissolved portions of the mixture of the flake and liquid.
A23J 1/20 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from milk, e.g. caseinObtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from whey
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
4.
METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION OF EXOGENOUS CASEINS IN TRANSGENIC PLANTS
The present disclosure provides methods and plants by genetically modifying plants to enhance their nutritional quality. In some aspects, the present disclosure provides methods for reducing activity or expression of one or more proteases in a plant (e.g., soybean), leading to increased levels of casein proteins and casein micelles, resulting in plants with enhanced levels of essential amino acids and improved digestibility.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
C12Q 1/6895 - Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
G01N 33/68 - Chemical analysis of biological material, e.g. blood, urineTesting involving biospecific ligand binding methodsImmunological testing involving proteins, peptides or amino acids
5.
GLYCELLES: METHODS AND COMPOSITIONS FOR CASEIN MICELLES COMPRISING NON-BIOACTIVE HYDROPHILIC COMPOUNDS
By integrating casein proteins with non-milk particles (for example, soy proteins), Glycelles achieve enhanced nutritional value, improved digestibility, and superior sensory attributes. This innovation addresses challenges associated with non-milk particles such as soy proteins, offering a sustainable, versatile, and healthful protein source with broad applications in the food, beverage, nutraceutical, sports nutrition, and dietary supplement sectors.
By integrating casein proteins with non-milk particles (for example, soy proteins), Glycelles achieve enhanced nutritional value, improved digestibility, and superior sensory attributes. This innovation addresses challenges associated with non-milk particles such as soy proteins, offering a sustainable, versatile, and healthful protein source with broad applications in the food, beverage, nutraceutical, sports nutrition, and dietary supplement sectors.
01 - Chemical and biological materials for industrial, scientific and agricultural use
42 - Scientific, technological and industrial services, research and design
Goods & Services
Proteins for use in the manufacture of food products; proteins for use in the manufacture of beverage products; casein to be used in the manufacture of food products; casein to be used in the manufacture of beverage products; biological preparations for use in the production of agricultural seeds Product research and development for the food ingredient and food industry; product development consultation and product research and development services for others in the field of food and beverage ingredients; engineering services and technical consultancy related to the production of proteins using plants; scientific consultancy and advisory services relating to the scientific analysis and development of non-animal based proteins for use in food and beverages
8.
COMPOSITIONS, METHODS AND SYSTEMS FOR PRODUCTION OF EGG WHITE PROTEINS IN PLANTS
Compositions, methods and systems for producing recombinant egg white proteins, as well as transgenic plants and food compositions comprising recombinant egg white proteins, including tagged proteins comprising egg white proteins with additional amino acids. Egg white proteins can be mixed with plant proteins to produce food products.
Compositions, methods and systems for producing recombinant egg white proteins, as well as transgenic plants and food compositions comprising recombinant egg white proteins, including tagged proteins comprising egg white proteins with additional amino acids. Egg white proteins can be mixed with plant proteins to produce food products.
A23L 15/00 - Egg productsPreparation or treatment thereof
A23J 1/14 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seedsObtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from press-cake or oil-bearing seeds
C07K 14/465 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from birds
Compositions, methods and systems for producing functional non-glycosylated, under- glycosylated, or differentially-glycosylated recombinant casein proteins in plants which allow for the safe, sustainable, and humane production of milk proteins for commercial use, such as in food compositions. The disclosure provides modified non-glycosylated, under-glycosylated, or differentially-glycosylated recombinant casein protein, or fragments thereof. The disclosure also provides methods for producing modified non-glycosylated, under-glycosylated, or differentially- glycosylated recombinant proteins, and food compositions comprising the same.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
C12P 21/02 - Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Compositions, methods and systems for making cheese using a soy ingredient having one or more soy proteins. In some instances, the soy protein is a storage protein comprising at least one of 7s (e.g., 0-conglycinin) or I ls (e.g., glycinin). In some cases, the ratio of 7s/l Is is lower than a naturally occurring ratio. In some aspects, current disclosure provides a new composition of milk augmented with soy proteins that minimizes the negative effects of soy on coagulation of casein micelles. In some instances, the soy ingredient is modified to decrease the ratio of 7s (e.g., 0- conglycinin) to 1 Is (e.g., glycinin), resulting in improved coagulation of casein micelles and cheese production.
A23J 1/20 - Obtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from milk, e.g. caseinObtaining protein compositions for foodstuffsBulk opening of eggs and separation of yolks from whites from whey
A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a κ-casein and at least one of an αS1-casein, an αS2-casein, a β-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the κ-casein, an inner matrix of the recombinant micelle include at least one of the αS1-casein, the αS2-casein, the β-casein.
C07K 14/47 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from animalsPeptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof from humans from vertebrates from mammals
C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
A plant cell co-expressing at least one casein protein and at least one kinase. The at least one casein protein is phosphorylated by the at least one kinase in vivo. Casein micelles comprising phosphorylated κ-casein and at least one of αS1-casein, αS2-casein, and β-casein can be made in vivo and/or in vitro. The casein micelles can be used to make food products including milk and cheese.
A method of in vivo assembly of a recombinant micelle including: introducing a plasmid into a plant cell, wherein: the plasmid includes a segment of deoxyribonucleic acid (DNA) for encoding a ribonucleic acid (RNA) for a protein in a casein micelle, the segment of DNA is transcribed and translated; forming recombinant casein proteins in the plant cell, wherein: the recombinant casein proteins include a ?-casein and at least one of an aS1-casein, an aS2-casein, a ß-casein; and assembling in vivo a recombinant micelle within the plant cell, wherein: an outer layer of the recombinant micelle is enriched with the ?-casein, an inner matrix of the recombinant micelle include at least one of the aS1-casein, the aS2-casein, the ß-casein.
