Abstract

in vitro in vitro cell-free biosynthesis of the cofactor, coenzyme A ("CoA") from lower-cost substrates, such as D-pantothenate, L-cysteine, and ATP, and the use of these pathways in CoA-dependent cell-free systems for the biobased production of chemicals, such as acetyl-CoA, hexanoyl-CoA, malonyl-CoA, and cannabigerolic acid (CBGA).

IPC Classes  ?

• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 13/12 - MethionineCysteineCystine

Abstract

The application relates to recombinant polypeptides having berberine bridge enzyme (BBE) activity that are derived from microorganisms, such as Phytohabitans sufuscus, Streptomyces sp. AJS327, Streptomyces varsoviensis, Actinomadura pelletieri, Streptomyces flaveolus, Streptomyces sp. Ru71. or Streptomyces sp. CNH287, and the use of these recombinant polypeptides in compositions and methods for the oxidative cyclization of prenylated compounds, such as CBGA, in carrying out the biosynthesis, including cell-free biosynthesis, of cyclized cannabinoid compounds, such as CBCA, THCA, and CBDA.

IPC Classes  ?

• C07D 311/70 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulfur atoms in position 2 or 4 with two hydrocarbon radicals attached in position 2 and elements other than carbon and hydrogen in position 6
• C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/10 - Transferases (2.)
• C12N 9/88 - Lyases (4.)
• C12P 17/06 - Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Abstract

The present disclosure relates to compositions comprising cyclolavandulyl derivatives of aromatic compounds, such as cannabinoids, flavonoids, and alkaloids, and methods for preparing and using the compositions.

IPC Classes  ?

• C12P 7/22 - Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
• C12N 9/10 - Transferases (2.)
• C12P 17/06 - Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein

Abstract

The present disclosure relates to multi-enzyme pathways for the in situ generation of the cofactor NADP from lower-cost reagents, such as nicotinate, NMN (nicotinamide mononucleotide), and NAD (nicotinamide adenine dinucleotide), and the use of these pathways in NADP-dependent cell-free systems for the biobased production of chemicals, such as isobutanol.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12N 9/12 - Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
• C12P 13/06 - AlanineLeucineIsoleucineSerineHomoserine
• C12P 13/08 - LysineDiaminopimelic acidThreonineValine
• C12P 19/36 - Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
• C12P 7/16 - Butanols

Abstract

Systems and methods for prenylation and recombinant pathways for the production of compounds, cannabinoids, cannabinoid precursors, and other prenylated chemicals in a cell free system. The present invention further includes enzymes and recombinant microorganisms that catalyze the reaction. The compounds, cannabinoids, cannabinoid precursors, and other prenylated chemicals are operable to be substituted with at least one deuterium, at least one tritium, at least one halogen, at least one hydroxyl group, and/or at least one additional isotope.

IPC Classes  ?

• A61K 31/00 - Medicinal preparations containing organic active ingredients
• C12P 17/18 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin

Abstract

The present disclosure relates to compositions comprising cyclolavandulyl derivatives of aromatic compounds, such as cannabinoids, flavonoids, and alkaloids, and methods for preparing and using the compositions.

IPC Classes  ?

• C07C 13/20 - Monocyclic hydrocarbons or acyclic hydrocarbon derivatives thereof with a six-membered ring with a cyclohexene ring
• C07C 13/18 - Monocyclic hydrocarbons or acyclic hydrocarbon derivatives thereof with a six-membered ring with a cyclohexane ring
• A61K 31/015 - Hydrocarbons carbocyclic
• C07C 5/00 - Preparation of hydrocarbons from hydrocarbons containing the same number of carbon atoms

Abstract

, Phytohabitans suffuscusStreptomyces sp.Streptomyces varsoviensisActinomadura pelletieriStreptomyces flaveolusStreptomyces sp.Streptomyces sp.Streptomyces sp. CNH287, and the use of these recombinant polypeptides in compositions and methods for the oxidative cyclization of prenylated compounds, such as CBGA, in carrying out the biosynthesis, including cell-free biosynthesis, of cyclized cannabinoid compounds, such as CBCA, THCA, and CBDA.

IPC Classes  ?

• C12N 9/02 - Oxidoreductases (1.), e.g. luciferase
• C12Q 1/26 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving oxidoreductase
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C07D 311/76 - Benzo [c] pyrans
• C07D 311/58 - Benzo [b] pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulfur atoms in position 2 or 4
• C07C 39/19 - Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring monocyclic with unsaturation outside the aromatic ring containing carbon-to-carbon double bonds but no carbon-to-carbon triple bonds

Abstract

The present disclosure relates to recombinant prenyltransferase enzymes with increased thermostability and activity and the use of these enzymes in compositions and methods for biosynthesis involving prenylation reactions, including compositions and methods for the preparation of cannabinoids.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12N 15/52 - Genes encoding for enzymes or proenzymes
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12P 7/42 - Hydroxy carboxylic acids

Abstract

The present disclosure relates to recombinant prenyltransferase enzymes with increased thermostability and activity and the use of these enzymes in compositions and methods for biosynthesis involving prenylation reactions, including compositions and methods for the preparation of cannabinoids.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12P 7/42 - Hydroxy carboxylic acids
• C12N 15/82 - Vectors or expression systems specially adapted for eukaryotic hosts for plant cells
• C12N 15/52 - Genes encoding for enzymes or proenzymes

Abstract

The present disclosure relates to recombinant prenyltransferase enzymes with increased thermostability and activity and the use of these enzymes in compositions and methods for biosynthesis involving prenylation reactions, including compositions and methods for the preparation of cannabinoids.

IPC Classes  ?

• C12N 9/10 - Transferases (2.)
• C12P 5/00 - Preparation of hydrocarbons
• C12P 7/00 - Preparation of oxygen-containing organic compounds
• C12P 17/00 - Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms

Goods & Services

Fragrances; terpenes for use in essential oils Biofuels; fuels, biofuels and biodiesels based on energy-producing organic compounds, namely, terpenes, isoprenoids, alcohols, fatty acids, esters, alkanes, and alkenes Pharmaceuticals, namely, alcohols, esters, alkaloids, polyketides, steroids, isoprenoids, and non-ribosomal peptides, for use the treatment of pain, neuropathic pain, cancer pain, oncology, cancer symptoms, psychiatric illnesses, neurodegenerative diseases, multiple sclerosis, spinal cord injury, bladder disfunction, peripheral neuropathy, spasticity, perioperative pain, rheumatoid arthritis, inflammatory bowel disease, ulcerative colitis, hypoxic-ischaemic encephalopathy, symptoms of neurogenic disorders, movement disorders, essential tremors, psychotic disorders, diseases of the central nervous system, stroke and head injuries, motion sickness and chemically induced sickness, post-traumatic stress disorder, obesity, obesity associated with Type II Diabetes, bulimia, schizophrenia, Crohn's disease, Alzheimer's disease, bone disorders, inflammatory disorders, sleep and sleep disorders, and drug, alcohol, and nicotine abuse, epilepsy, convulsions, seizures, Dravet syndrome, Lennox-Gastaut syndrome, intractable childhood epilepsy with generalized tonic-clonic seizures, generalized epilepsy with febrile seizures plus, Doose syndrome, chromosome disorders, and metabolic disorders; anti-inflammatories; analgesics; nutraceuticals for use as a dietary supplement; dietary and nutritional supplements; none of the foregoing containing CBD, cannabinoids or tryptamines Flavor enhancers for use in food and beverage products

Goods & Services

Organic compounds for use in biotechnological and manufacturing processes and product development; organic compounds for use in the biochemical and chemical industries; organic compounds, namely, alcohols, fatty acids, esters, alkanes, alkenes, organic acids, indoles, flavonoids, flavonoids, polyketides, carotenoids, non-ribosomal peptides, lactones, aldehydes, alkaloids, isoprenoids, insect hormones, and plant hormones, for use in biotechnological manufacturing processes; organic compounds for use in biochemical and chemical industries, namely, pharmaceutical, energy, biofuel, food, beverage, dietary, nutritional, nutraceutical, flavor, fragrance, insect control, and agrochemical industries; organic compounds for use in the manufacture of biofuels; organic compounds for use in the manufacture of dietary and nutritional supplements; organic compounds for use in the manufacture of food and beverage products; none of the foregoing containing CBD, cannabinoids or tryptamines