Abstract

Reagents and methods are provided that allow for art Improved expression of a recombinant protein. More specifically, the Introduction of recombinant DNA elements into a host coll allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in cell viability and proliferation of the host cell. These recombinant DNA elements can be introduced into host cells, for example, via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.

IPC Classes  ?

• C12N 7/01 - Viruses, e.g. bacteriophages, modified by introduction of foreign genetic material
• C12N 15/63 - Introduction of foreign genetic material using vectorsVectorsUse of hosts thereforRegulation of expression
• C12N 15/33 - Genes encoding viral proteins
• C12N 15/10 - Processes for the isolation, preparation or purification of DNA or RNA
• C12P 21/00 - Preparation of peptides or proteins
• C07K 14/01 - DNA viruses

Abstract

Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and lE-1, transcriptional enhancer elements, such as the homologous region (hr) and promoters.

IPC Classes  ?

• A01K 67/033 - Rearing or breeding invertebrates; New breeds of invertebrates
• C12N 15/33 - Genes encoding viral proteins
• C12P 21/00 - Preparation of peptides or proteins
• C07K 14/01 - DNA viruses

Abstract

New antiviral peptides interfering the binding of the virus to DLC8 are provided. A high number of pathogenic agents of viral origin use the dynein based intracellular transport machinery at some point of their infective cycle. The present invention consists of a new antiviral therapy consisting in the inhibition of viral infections produced by those virus that use the dynein system by mechanisms of interference mainly by preventing the interaction between the viral protein and the cellular DLC8 protein. The present invention discloses for the first time the blocking of the function of this interaction by peptides whose sequence comprises or consists of the totality or a partial sequence of the viral protein corresponding to the binding domain with DLC8.

IPC Classes  ?

• C07K 14/00 - Peptides having more than 20 amino acidsGastrinsSomatostatinsMelanotropinsDerivatives thereof
• A61K 38/00 - Medicinal preparations containing peptides

Abstract

Human papillomavirus like structures produced in a non-fermentative system based on insect larva. Sexually transmitted human papillomavirus (HPV) infections cause most cervical cancers. The inability to propagate HPV in vitro has led to the development of alternative approaches to antigen production for vaccine development. In the present invention we describe a low-cost efficient production of HPV pentamers or virus-like particles (VLPs) inanon-fermentative system based on baculovirus and Tήchoplusia ni (T. ni) insects as living biofactories. Th e L1 protein from HPV 16 was expressed by a recombinant baculovirus in larva at levels that reached 18 to 21 mg per g of fresh insect biomass. It represents about 2.5 times the L1 production yields obtained per g of insect cells using the same recombinant baculovirus. Under electron microscopy, purified VLPs produced in larvae were indistinguishable from those produced in Sf-21 insect cell cultures, presenting identical shape and size. Immunization of mice with the insect-derived VLPs induced a potent immune response similar to that obtained with insect cells-derived VLPs.

IPC Classes  ?

• A01K 67/033 - Rearing or breeding invertebrates; New breeds of invertebrates
• A61K 39/295 - Polyvalent viral antigensMixtures of viral and bacterial antigens
• C12N 15/866 - Baculoviral vectors

Abstract

Insect-derived promoters for foreign proteins expression in insect cells. Regulatory polynucleotide sequences which drive the expression of major insect proteins (hexamerins) at specific evolution stages of larva have been isolated from insects (Trichoplusia ni) in the present invention. Said regulatory polynucleotide sequences promote stronger foreign gene expression in the baculovirus system than the conventional polyhedrin promoter. Additionally, the combination of the new larva-derived promoters of the invention with the pL promoter increased baculovirus expression levels up to 61%-375% (depending on the time of infection) with respect to conventional baculo viruses used in the biotechnology industry. Promoter pB2 also drives gene expression at earlier times than polyhedrin promoter, being this feature a great advantage for correct protein folding and posttranslational modification.

IPC Classes  ?

• C12N 15/85 - Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
• C12N 15/866 - Baculoviral vectors
• A01K 67/033 - Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

Monomeric VHH domain derived from anti-VP6 camelid antibodies, dimeric domain, immunisation method, rotavirus detection method, vaccine composition, prevention and treatment methods for rotavirus infections, wherein said domain may be any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 or SEQ ID No. 4, and wherein said domains bind to protein VP6 of Group A rotavirus.

IPC Classes  ?

• C07K 16/10 - Immunoglobulins, e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
• A61P 1/06 - Anti-spasmodics, e.g. drugs for colics, esophagic dyskinesia
• A61P 31/14 - Antivirals for RNA viruses