A portable diagnostic device has a lysate stage (167) with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages (161-164) are linked with the lysate vessel, each with a reservoir linked by channels. The final stage (164) has a sensor (169) for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.
An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
3.
RNA TARGET ENRICHMENT OR DEPLETION OF BIOLOGICAL SAMPLES
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
A portable diagnostic device has a lysate stage (167) with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages (161-164) are linked with the lysate vessel, each with a reservoir linked by channels. The final stage (164) has a sensor (169) for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.
An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
6.
SINGLE-BEAD CAPACITIVE DETECTOR FOR MICROBIOLOGICAL APPLICATIONS
The present invention provides an improved capacitive bead sensor for detection and/or quantification of target analytes in a sample, with a detection limit down to single-beads, which is re-usable for multiple bead tests, or for a continuous flow of beads, and which is easily manufacturable and automatable. It enables sensitivity down to single molecule detection without the need for enzymatic amplification such as PCR, by use of various structural advantages and electronic signal amplification techniques that further allow for multiplex target detection not only across various nucleic acid targets but across entire target classes allowing for simultaneous detection of viral nucleic acids and host antibodies to that virus for example.
C12Q 1/6837 - Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01N 33/543 - ImmunoassayBiospecific binding assayMaterials therefor with an insoluble carrier for immobilising immunochemicals
The present invention provides an improved capacitive bead sensor for detection and/or quantification of target analytes in a sample, with a detection limit down to single-beads, which is re-usable for multiple bead tests, or for a continuous flow of beads, and which is easily manufacturable and automatable. It enables sensitivity down to single molecule detection without the need for enzymatic amplification such as PCR, by use of various structural advantages and electronic signal amplification techniques that further allow for multiplex target detection not only across various nucleic acid targets but across entire target classes allowing for simultaneous detection of viral nucleic acids and host antibodies to that virus for example.
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES (USA)
Inventor
O'Farrell, Brian
O'Connell, Claire
Oshaben, Kaylyn
Appella, Daniel
Abstract
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES (USA)
Inventor
O'Farrell, Brian
O'Connell, Claire
Oshaben, Kaylyn
Appella, Daniel
Abstract
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
C07H 21/00 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
The United States of America, as represented by the Secretary, Department of Health and Human Services (USA)
Inventor
O'Farrell, Brian
O'Connell, Claire
Oshaben, Kaylyn
Appella, Daniel
Abstract
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or “tethers” the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.
An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK (Ireland)
ALTRATECH LIMITED (Ireland)
Inventor
O'Farrell, Brian
O'Connell, Ivan
Darby, Steven
Cummins, Timothy
Abstract
A diagnostic sensor device has a semiconductor chip having a distal end physically configured to fit into a power and data socket conforming to a non-proprietary standard, and having exposed pads for engagement with corresponding conductors of such a socket. At its proximal end the chip has at least one sensor for contact with an analyte. The device may be manufactured in a single integrated process to provide a wafer which is diced to provide the individual devices.
A portable diagnostic device has a lysate stage with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages are linked with the lysate vessel, each with a reservoir linked by channels. The final stage has a sensor for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.
UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK (Ireland)
ALTRATECH LIMITED (Ireland)
Inventor
Cummins, Timothy
O'Farrell, Brian
Darby, Steven
O'Connell, Ivan
Abstract
A diagnostic sensor device (1) has a semiconductor chip (2) having a distal end physically configured to fit into a power and data socket (10) conforming to a non-proprietary standard, and having exposed pads (3) for engagement with corresponding conductors of such a socket. At its proximal end the chip has at least one sensor (5) for contact with an analyte. The device may be manufactured in a single integrated process to provide a wafer (9) which is diced to provide the individual devices.
A portable diagnostic device has a lysate stage (167) with a port for receiving a sample and containing magnetic beads with a probe, and an outlet port. A series of assay stages (161-164) are linked with the lysate vessel, each with a reservoir linked by channels. The final stage (164) has a sensor (169) for detecting beads attached to analyte molecules which have been conveyed according to attachment to probes on beads. Larger transport beads cause reporter beads which are tethered by target NA and probes to be transported to the final sensor stage, where they are released and detected when the transport beads have been removed.
A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.
A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel, within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads which are initially embedded in a wax body and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket along with the wax, which is allowed to solidify before they are removed from the reaction vessel.
An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
G01N 27/74 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or “tethers” the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.
A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel (11), within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads (26) which are initially embedded in a wax body (17) and are released during the heating so that they are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket (16) along with the wax (17), which is allowed to solidify before they are removed from the reaction vessel.
A sampling apparatus (1) has a tubular stem (2) at the distal end of which are four axially- extending teeth (5) forming a distal tip. There is a recess (10) within the tip, within which there is an axial spike (6). There are two rectangular openings (7) in the wall of the stem at the recess (10). The apparatus (1) is moved to approach a bulk material (M) until the distal tip, including the spike (6) penetrates the material. The guide pushes some sample material across the recess towards the openings (7). There may be a rotating action to assist separation of a plug or sample from the bulk material. Upon withdrawal, the sample may then be inserted into a receptacle for analysis, preferably with being brought into contact with a lysis buffer or other liquid reagent.
Methods of detecting target nucleic acid is a sample are described. A first probe is attached to first beads, and the first beads are placed in the sample so that any target nucleic acid attaches to the first probe. A second probe also attaches to the target nucleic acid so that any of the target nucleic acid links or "tethers" the first and second probes. A capacitive sensor detects capacitance of the beads and processes capacitance data to quantify target nucleic acid presence in the sample. The second probe may be immobilised on the sensor surface. Alternatively the second beads are introduced into the sample with the second probe attached, and the extent of tethering of the first beads to the second beads is indicative of the extent of target NA present.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
G01R 27/26 - Measuring inductance or capacitanceMeasuring quality factor, e.g. by using the resonance methodMeasuring loss factorMeasuring dielectric constants
A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel (11), within which is added a chelating agent to a sample with heating to about 99° C to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads (26) which are initially embedded in a wax body (17) and are released during the heating so that the y are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket (16) along with the wax (17), which is allowed to solidify before they are removed from the reaction vessel.
An analyte in a liquid sample is detected using a capacitive sensor having electrodes and a sensor surface, and a signal processor. The sample is dried to reduce its liquid content, and capacitive measurements are made after the drying and preferably also before the drying. The sample may include particles, and the analyte is part of or attached to the particles, and the particles provide a major part of the capacitance change compared to absence of particles. In another example the particles are degenerative and form an integral mass upon application of heat, enhancing the extent of capacitance change.
C12Q 1/68 - Measuring or testing processes involving enzymes, nucleic acids or microorganismsCompositions thereforProcesses of preparing such compositions involving nucleic acids
G01N 27/22 - Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
01 - Chemical and biological materials for industrial, scientific and agricultural use
09 - Scientific and electric apparatus and instruments
10 - Medical apparatus and instruments
Goods & Services
Chemicals used in industry, science and agriculture; chemical preparations for analyses in laboratories, other than for medical or veterinary purposes; chemical reagents, other than for medical or veterinary purposes; chemical reagents for scientific and diagnostic purposes; assays for use in testing, identification and analysis; assays for scientific and diagnostic purposes; DNA testing kits and apparatus for scientific and diagnostic purposes; chemicals for use in DNA testing, identification and analysis; chemical reagents for use in DNA testing, identification and analysis; chemical testing kits for use in testing, identification and analysis; chemicals for use in genetic testing and genetic research; chemical reagents for use in genetic testing and genetic research; chemical testing kits for use in genetic testing and genetic research; reagents for use in scientific apparatus for chemical or biological analysis; reagents and assays for diagnostic use and for use in DNA testing, identification and analysis; diagnostic preparations for scientific purposes; diagnostic reagents for scientific use; diagnostic kits for sample testing purposes; chemical testing kits for scientific and research purposes; chemical testing kits for use in detection of viruses in hospitals, detection of toxins in food and perishable products, and monitoring of food and pharmaceutical manufacturing processes; catalysts for use in chemical and biochemical processes; biochemical catalysts. Scientific, surveying, optical, weighing, measuring, signalling and checking (supervision) apparatus and instruments; measuring apparatus; measuring instruments; measuring devices, electric; monitoring apparatus, electric; electronic and electric measuring devices and instruments not for medical purposes; testing apparatus not for medical purposes; diagnostic apparatus and instruments, other than for medical or veterinary use; scientific apparatus and instruments; chemistry apparatus and instruments; apparatus and instruments for scientific research in laboratories; laboratory apparatus and instruments; laboratory glassware; test tubes; laboratory plates and microplates; reaction cassettes for use in laboratory analysis; microfluidic devices and instruments; capacitive sensors; biosensing apparatus and instruments; semiconductors; semiconductor chips; semiconductor DNA detection chips; DNA chips; gene chips; biochips; apparatus and instruments for use in sample preparation; apparatus and instruments for use in food security applications; apparatus and instruments for use in DNA testing; apparatus and instruments for use in biological testing; apparatus and instruments for use in genetic testing; apparatus and instruments for use in molecular biology; apparatus and instruments for use in biological processing; apparatus and instruments for use in biological analysis; diagnostic and screening kits, apparatus and instruments for use in DNA testing, identification and analysis; diagnostic reagents and assays, other than for medical or veterinary use, for use in kit form; electronic publications, downloadable; publications in electronic format; computer software; application software; USB flash drives; magnetic data carriers, recording discs; compact discs, DVDs and other digital recording media; data processing equipment; computers; parts and fittings for the aforesaid goods. Surgical, medical and veterinary apparatus and instruments; medical apparatus and instruments; medical devices; testing apparatus and instruments for medical purposes; diagnostic apparatus and instruments for medical purposes; medical testing kits; apparatus for use in medical testing and analysis; blood testing apparatus; testing, measuring and detecting instruments for medical use; devices for sensing, detecting, measuring and monitoring for medical purposes; medical analysis instruments; detecting instruments for medical use; sensors for medical use; sensor apparatus for medical use in diagnosis; chemical testing kits for use in testing, identification and analysis for medical purposes; apparatus and instruments for use in DNA testing, identification and analysis for medical purposes; medical diagnostic and screening kits and apparatus; instruments for diagnostic use and for use in screening for medical, agricultural or veterinary purposes; biosensing instruments and apparatus for medical purposes; parts and fittings for the aforesaid goods.
