The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.
C12P 7/18 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
C07H 21/04 - Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
2.
Methods and genetically engineered micro-organisms for the combined production of PDO, BDO and PHP by fermentation
The present invention relates to genetically engineered micro-organisms for the combined production of 1,3-propanediol (PDO), 2,3-butanediol (BDO), and polyhydroxypropionic acid (PHP) by fermentation. In particular, the invention relates to a genetically engineered micro-organism suitable for combined production of PDO, BDO and PHP by fermentation, characterized in that: compared with corresponding wild-type starting micro-organism, the D-lactate dehydrogenase gene in the genetically engineered micro-organism is deleted or functionally inactivated, and the genetically engineered micro-organism comprises a heterogenous polynucleotide encoding the Coenzyme A-dependent Aldehyde dehydrogenase and a heterogenous polynucleotide encoding the Polyhydroxyalkanoate synthase. Methods for the construction of such micro-organisms, and methods for combined production of PDO, BDO and PHP by fermentation of a genetically engineered bacterium are also taught.
C12P 7/18 - Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
C12N 9/00 - Enzymes, e.g. ligases (6.)ProenzymesCompositions thereofProcesses for preparing, activating, inhibiting, separating, or purifying enzymes
C12N 15/00 - Mutation or genetic engineeringDNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purificationUse of hosts therefor
3.
A METHOD FOR CO-PRODUCTION OF 1, 3-PROPANEDIOL, 2,3-BUTANEDIOL AND POLYHYDROXYPROPIONIC ACID BY FERMENTATION OF CONSTRUCTED GENETIC ENGINEERING BACTERIA
A method for co-production of 1,3-propanediol (PDO), 2,3-butanediol (BDO) and polyhydroxypropionic acid (PHP) via fermentation by genetic engineering bacteria comprises the following steps of: knocking out D type lactate dehydrogenase from wild PDO producing bacteria, introducing coenzyme A depending aldehyde dehydrogenase and polyhydroxy fatty acid synthase genes, constructing genetic engineering bacteria for producing PDO, BDO and PHP; fermenting aerobically and adjusting fermentation by adding mixture flow of glycerin and base solution; filtering fermentation broth by membrane, treating it by electrodialysis, concentrating, rectifying which separates PDO, BDO and PHP. The said genetic engineering bacteria can produce PDO, BDO and PHP at the same time, which increases utilization ratio of raw material, reduces cost, decreases generation of byproduct lactate, simplifies post extraction technique, lowers extraction cost, and increases generation of nicotinamide adenine dinucleotide 2 (NADH2) while PHP is introduced.
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