Abstract

A method for controlled production of glyceric acid includes, during the glyceric acid fermentation process, real-time online monitoring of at least one of the respiratory quotient and redox potential in the fermentation liquid as well as the fermentation process specific growth rate, and controlling their values within the following ranges: 0.1˜1.5 for respiratory quotient, −300˜50 mV for redox potential, 0.05˜0.8 for specific growth rate. Process parameters for this method are controlled within predetermined ranges by online monitoring at least one of respiratory quotient (RQ) and redox potential as well as the specific growth rate in the fermentation process, thereby meeting the growth requirements of bacteria, effectively promoting the glycerol metabolism and synthesis and improving the conversion rate.

IPC Classes  ?

• C12P 7/42 - Hydroxy carboxylic acids
• C12N 1/20 - BacteriaCulture media therefor

Abstract

The present invention pertains to the field of nutrition and medicine, and relates to a drug combination for anti-aging and its use. Specifically, the present invention relates to a drug combination comprising: 0.5 to 30 parts by weight of NR or its pharmaceutically acceptable salt, 0.5 to 30 parts by weight of NMN, and 0.01 to 35 parts by weight of mineral. The drug combination of the present invention has good anti-oxidative or anti-aging effects, and has excellent stability.

IPC Classes  ?

• A61K 31/706 - Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
• A61K 9/50 - Microcapsules
• A61K 33/04 - Sulfur, selenium or telluriumCompounds thereof
• A61K 33/08 - OxidesHydroxides
• A61K 33/30 - ZincCompounds thereof
• A61K 36/06 - Fungi, e.g. yeasts
• A61K 45/06 - Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca

Abstract

The invention belongs to the field of fermentation engineering, and relates to a method for producing arachidonic acid. The method comprises: successively subjecting Mortierella alpine or mutant strains thereof to strain activation, seed amplification culture and fermentation culture, wherein OUR is monitored online, and the OUR value is controlled at 10-100 mmol/L.h during the fermentation culture, and/or after 100 h of fermentation culture, ORP is monitored in fermentation liquid online, and the ORP value is controlled to be within the range of 50-150 mv. By adopting the method, the fermentation production level can be stably improved, such that the content of the arachidonic acid in the obtained fermentation solution is obviously improved.

IPC Classes  ?

• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12N 1/14 - Fungi Culture media therefor
• C12Q 3/00 - Condition-responsive control processes
• C12R 1/645 - Fungi

Abstract

A method for preparing vitamin K2 by microbial fermentation, comprising: controlling, in stages, at least one among oxygen uptake rate, oxidation-reduction potential in the fermentation liquid, and lactic acid concentration within a predetermined range; and/or, after 5 to 20 hours of fermentation, adding auxiliary materials into the fermentation liquid, said auxiliary materials being selected from at least one among nicotinamide, vitamin B12 and methionine. Preparation of vitamin K2 by means of the described method satisfies different requirements for the culturing conditions for vitamin K2 fermentation in the stages of growth, synthesis etc., promoting bacterial growth and product synthesis, efficiently improving the fermentation level of vitamin K2, and achieving high vitamin K2 content in the fermentation product.

IPC Classes  ?

• C12P 7/66 - Preparation of oxygen-containing organic compounds containing the quinoid structure
• C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound
• C12N 1/20 - BacteriaCulture media therefor
• C12R 1/125 - Bacillus subtilis
• C12R 1/20 - Flavobacterium
• C12R 1/10 - Bacillus licheniformis
• C12R 1/07 - Bacillus

Abstract

Disclosed is a method for separating coenzyme Q10 by means of using a supercritical fluid chromatography system, the method comprising: (1) starting a supercritical fluid chromatography system and setting system parameters; (2) once the system is operating stably, a chromatographic column is sufficiently balanced and a baseline is stable, injecting a coenzyme Q10 feeding solution and collecting a coenzyme Q10 component according to an ultraviolet peak signal at an output peak; and (3) removing a solvent from the coenzyme Q10 component to obtain high-purity coenzyme Q10. By means of the method, a supercritical fluid chromatography system-based separation method is used to treat a crude coenzyme Q10 extract for the first time and the coenzyme Q10 can be successfully separated from homologs thereof; the obtained coenzyme Q10 has relatively high purity and yield; and the method has low organic waste liquid emissions and a low amount of pollution, consumes a short amount of time, and has high separation efficiency, and has the characteristics of being economical and highly efficient, having easy operations, and being environmentally friendly.

IPC Classes  ?

• C07C 46/10 - SeparationPurificationStabilisationUse of additives
• C07C 50/06 - Quinones with monocyclic quinoid structure with unsaturation outside the quinoid structure
• G01N 30/02 - Column chromatography

Abstract

A simulated moving bed continuous chromatography system and an application thereof, and a method for purifying coenzyme Q10. The simulated moving bed continuous chromatography system comprises at least four chromatographic columns sequentially communicated end to end. A feed liquid inlet, an eluent inlet 1#, an eluent inlet 2# and an eluent inlet 3# are sequentially arranged in the direction of chromatographic column arrangement. The simulated moving bed continuous chromatography system is divided into a feed zone, an elution zone, a desorption zone and a regeneration zone by means of the four material inlets, and by switching a porous distribution valve, the chromatographic columns are sequentially and cyclically switched to the feed zone, the elution zone, the desorption zone and the regeneration zone.

IPC Classes  ?

• B01D 15/18 - Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
• B01D 15/42 - Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
• C07C 46/10 - SeparationPurificationStabilisationUse of additives
• C07C 50/28 - Quinones containing groups having oxygen atoms singly bound to carbon atoms with monocyclic quinoid structure

Abstract

The present invention relates to the field of polyunsaturated fatty acid treatment, and disclosed thereby are a hybrid adsorbent and an application thereof, and a method and equipment for treating polyunsaturated fatty acid. Said hybrid adsorbent contains aluminum oxide and copper salt modified zeolite molecular sieves, and preferably further contains at least one of argil, attapulgite, silicon dioxide and activated carbon. The aluminum oxide is neural aluminum oxide and/or acidic aluminum oxide. The hybrid adsorbent provided by the present invention is supplemented by ultrasonic activation, and accordingly is comparable to silica gel in the aspects of decolorization and aldehyde ketone quinone removal, and basically will not result in loss of other nutritional components in grease. Meanwhile, the merits of low cost, easy regeneration and good product stability are still retained.

IPC Classes  ?

• B01J 20/16 - Alumino-silicates
• B01J 20/34 - Regenerating or reactivating
• C11C 1/08 - Refining
• C11B 3/10 - Refining fats or fatty oils by adsorption

Abstract

A method for producing an arachidonic acid-containing mixed grease by utilizing industrialized fermentation of Mortierella alpina strain: starting from 100-140 hours into culturing in a fermentation tank, the fermentation temperature is controlled at 20-25 °C; and/or from the 8-10-th day of culturing in the fermentation tank, ventilation is stopped or the volume of ventilation is reduced by 50% or more.

IPC Classes  ?

• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12R 1/645 - Fungi
• C11B 1/02 - Pretreatment
• C11B 1/06 - Production of fats or fatty oils from raw materials by pressing

Abstract

The present invention falls within the field of fermentation engineering. Disclosed is a method for producing docosahexaenoic acid (DHA) by microbial fermentation. In particular, disclosed is a method for producing DHA-containing mixed grease by industrial fermentation of Schizochytrium limacinum strains.

IPC Classes  ?

• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C11B 1/06 - Production of fats or fatty oils from raw materials by pressing
• C11B 3/10 - Refining fats or fatty oils by adsorption
• C11B 3/12 - Refining fats or fatty oils by distillation
• C12R 1/645 - Fungi

Abstract

Schizochytrium. The method comprises steps of activation of a strain, preparation of a suspension of the strain, preparation of a primary seed, preparation of a secondary solid seed, enlarging cultivation of the secondary solid seed in a fermentor, collection of cells after fermentation, and extraction of DHA oil. With the fermentation method, the seed preparation method is improved, the seed enlarging technology is simplified, the period of seed culture and fermentation is shortened, and therefore the investment is reduced and the cost is saved; in addition, the solid culture medium is of eutrophy and rich in a plurality of growth factors, and is more beneficial to the growth of the seed; cells are strong in viability and good in synchronism in solid culture medium.

IPC Classes  ?

• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12R 1/89 - Algae

Abstract

Provided is a fermentation production method of docosahexenoic acid (DHA), in particular a liquid fermentation production method of DHA through solid material cultivation of the Schizochytrium. The method comprises steps of activation of the bacterium strain, preparation of bacterium suspension, preparation of primary seed, preparation of secondary solid material seed, enlarging cultivation of the secondary solid material seed in a fermentation tank, collection of thallus cells after fermentation, and extraction of DHA oil. In the fermentation method of the invention, the seed preparation method is improved; the seed enlarging technology is simplified; the period of seed culture and fermentation is shortened; and therefore the investment is reduced and cost is saved. In addition, the solid material culture medium is of eutrophy and rich in a plurality of growth factors and is more beneficial to the growth of the seed. Bacterium cells are strong in viability and good in synchronism. Besides, there are less waste water, waste residue and environmental pollution which are generated in the stage of solid material seed culture.

IPC Classes  ?

• C12P 7/64 - FatsFatty oilsEster-type waxesHigher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl groupOxidised oils or fats
• C12N 1/14 - Fungi Culture media therefor
• C12R 1/645 - Fungi

Abstract

A fermentation method for producing coenzyme Q10 is provided, including stepwisly culturing of microbial strains capable of producing coenzyme Q10, wherein key promoting factors are added in each stage of culture, and in the stage of culture in a fermentor, dissolved oxygen feedback-fed batch culture technique is adopted to realize the feedback regulation of the production of coenzyme Q10, so as to improve the yield of coenzyme Q10.

IPC Classes  ?

• C12P 7/66 - Preparation of oxygen-containing organic compounds containing the quinoid structure
• C12N 1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factorsStimulation of growth by removal of a chemical compound

Abstract

A fermentation method for producing co-enzyme Q10 is provided, comprising culturing strains capable of producing co-enzyme Q10 by fermentation stage by stage, wherein key promoters are added in each stage of fermentation and culture process, and in the fermentation period, dissolved oxygen feedback-fed batch culture is adopted to realize the feedback regulation of the production of co-enzyme Q10, so as to improve the yield of co-enzyme Q10.

IPC Classes  ?

• C12P 7/66 - Preparation of oxygen-containing organic compounds containing the quinoid structure
• C12R 1/01 - Bacteria or actinomycetales

Abstract

A regeneration method of chromatographic silica gel, especially a regeneration method of silica gel for chromatographing coenzyme Q10 is provided, wherein a high polar solvent is fed from the bottom of the chromatographic column to wash the column in the reverse direction. The treated chromatographic column can be repeatedly used to separate and purify coenzyme Q10. The regeneration method allows the repeated use of the chromatographic column for over 20 times, even over 100 times, while maintaining a good chromatographic effect. The method also has decreased production cost and labor intensity, and is safe and environment-friendly.

IPC Classes  ?

• B01J 20/283 - Porous sorbents based on silica
• B01J 20/34 - Regenerating or reactivating
• C07C 50/28 - Quinones containing groups having oxygen atoms singly bound to carbon atoms with monocyclic quinoid structure
• C07C 46/10 - SeparationPurificationStabilisationUse of additives